pycom01g02200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0000158
Physical Location & Seq
Forward (+)
1834258 .. 1838155
3898 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g02200.11

Sequence Viewer

Length: 2847 bp
ATGTTATATGTCACATCCCGGCCTGGGGCGGATCACTTCCCGGACCCGCTCCATCACCGTAGCACGATATTGTCCGCTTTGGGCTTACCATTCCCTCACGGTTTTGTTTTTGGGAACTCACAAGCAACTTCCCAGTGGGTCACCCATCATGGGATTGCTCTAGCCCTCTTCTCGCTTAACTTCGGAGTTCCTACGGAACCCGAAACCAGTGAGTTCCCAAAAAGCCTCGTGCTAGGTAGGGATGGGAATATACATTTAAGGATCACTTCCCTGGGCGATCCATGTGCACGTGCGGAAGCTAATAGAACAGTAATAAAAAAGGTACGAATAATTAAAAACTATCAGAGCAAGATTATAGCAGTCCAAAAGAAAAGTTGCAACAAAAGTACACCCGAAGGTGACCGTACGCTGGTGAGTATCTGTCAGAAAAGTCGGGAGAATCCTCTGGGAGACCACCGAAACTGCTACTCAACTAAAACCTGGAGGGGCACAAAACAAAAGCGTGAGTGGGCAAAAACAAATCTTTCGAAAACCATTCAAAATAAACATATACACATATGTATAGATATGCTAATCATGCTCAATTATATGCCATGCCGATGTCTCATAATAATATGCAAGTGTCCACATATAAACATATCAATAATATGTAATCTGAGAGCTCAAATCTCAATCTCAAAATCTAAACCTGCCCACGAGTCGGAACCACCTAAAGTGGTCTGTACGACAGGCCTGGGTGTAATCAAGTGCTACGATCACGTGAAGACTGGGCGAATAATCGCGGGTGCGGGAGGTGAACATCACGTGAAGGACTGTGCCCTGGCCACGGGCGGGAGCACTAACACTGGGGGTGCAGGTTATGAGCTCTCTAACTATGTCAAACCATTACTGAAACATAAACGTGAATACGCTTACCTGGCACTTACCTGTGCGTCCACAGCACCCAATATTCGACGATGCACGAATAACGATAAGGTAATGCATGGCATAACGAATAAACATTTATTCAATTTCTGGGAAAATATAGTATATAGAAGGGTCGTAGCCCCCCTGCCTCGCGTGTGCACGCTCGTCCTCGGCGTACGTGTCACCTATATGCGAAATAGCTATAAAAACGTTAACTTTAAAGCACATAACCTAACTTTCACCAACAATTTTTGGACCTCTCACGCGCCTCCACGCGCCAAAATAGGCACGGACCTACGCGCCCCCACGCGCGGCCACGTGCCATGCACACTGACAGTGTCAATTGACGCCGTCAGGAATATTCCGTTAGAAATCCAGAATATTCCGTTAAACTTAACCCCTGCCGTTAGGAATATTCCGTTAAACTTAACGGAATATTCCTGTTTCTTCTCCGGCCTGCCCTCGCCGGACTCCGGTCACCGGAAAACTGGCCGGAAAACTGGAAAACTTCAAACTCCCTATTCTCCTTCGTTTTTCATCCATTTTTCACGTAATTTATACCAAAATGAAGCCCTAAACATAATCTACCACGTTGGACAAGTTTTAGGGCCTAAAAACCACGGAATCAAACTTTCCAAAAATATGAAAATTCGGCCAAACTTACAGTTCGTGGTTCCGACGTCCAAATCCGTCCAACGATGCACTCCCAAGCTCCTAGGGACCTCACCAACACATTTACAAGCTTCAAAAGTTCAAAAACTAACTAGATTAAACTTGCATGAACAGTGCCCAAAACTGCCACTGTCGAAACGACGTGAAAACGTTAAATTCCCTCGATCTGTGAGGTTTTGGTGGGTTATGGGTTTGTCCGTACGTTTTAGGAAGAAGAAGAAGGATAGAGGACTCGGGGGAGAGAGAGACAGGGAGAAGACAGAGAGAGATGGCAGTGTGTTTGTGGGTGTGGCCCAACACCTGGGCAAAAACGTAATTTCGCACGTACGTTTCGATAATGTCGGGACGGGATGTCACATGGGATTTCACAATCCACCCCCCTTAGGGGCCCAGAAATGTGCCGACGATCACTTCCGGCCAGGGATTGGCTCTAATACCAATTTGTCACATCCCGGCCCGGGATTGTCCGCTTTGGGCTTACTATTCCCTCACGGTTTTGTTTTTGGGAACTCACGAGCAACTTCCCAGTGGGTCACCCATCATGGGATTGCTCTAACCCTCTTCTCGCTTAACTTCGGAGTTCCTACGGAACCCGAAACCAGTGAGCTCCCAAAAGGCCTCGTGCTAGGTAGGGATGGGAATATTCATTTAAGGATCACTTCCTTGGGCGATGTGGGATGTCACATTATATGCTTAAACGATAAGTCTAAGGAATATACCATTCTCTTTCGTATACATATCCTAAACGTTCCTGATCATAGGACTGCCAATTGGGCATTGACAACAGTGACTGGTCTCGAGTCATTGAATGAGTCCACTGAACGCGATCGACAAGGAGTTCTCATACTCATGTCACATGAAAACTCATGTTGTCTTATGGTTAGGTCCTTGATCTTTGACTATGTCAAAGTCACTCTATCAAAGGGTGTCCATGACATAGTTGGGGTTAAACCACTTAGCCATGGAGGGAGAATACTCTATGATATGATTAAGAATCTCTATCAAGGTAATCATATAAGTAAGAGAATCACATTGGATAAGTATTGTATTAGAGAAAGACCGTATTTAGCTTGGGTAGCCATGACATACTGCTACGTGTCACTCATGGTTTGTGGAAGCCCTAAAGTCACAATTGATTTGAAGATTTCTAATCGCCCACTGCCTCTCTTAAAGGAACCTAATGGATTGTACACTGTTAATATGTTAATTAATGAAACGACTAAGTTCGTTTTAGACCTCGGGTTAGTTTTGGGCCTCAAGGCCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

949

Amino Acids

105.91

Weight (kDa)

9.81

Isoelectric Point (pI)

36.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000158)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10163
malus_domestica MD04G1068000.v1.1 MD05G1130900.v1.1 MD06G1153600.v1.1 MD10G1255900.v1.1 MD10G1282000.v1.1 MD13G1075400.v1.1 MD13G1192400.v1.1
pyrus_communis pycom01g00130 pycom01g00390 pycom01g00700 pycom01g00720 pycom01g00750 pycom01g00850 pycom01g00870 pycom01g00910 pycom01g01250 pycom01g01560 pycom01g01680 pycom01g01860 pycom01g01900 pycom01g01910 pycom01g02200 pycom01g02260 pycom01g02270 pycom01g02280 pycom01g02330 pycom01g02480 pycom01g02720 pycom01g02730 pycom01g02750 pycom01g02770 pycom01g02780 pycom01g02860 pycom01g02910 pycom01g03540 pycom01g04090 pycom01g04100 pycom01g04150 pycom01g04250 pycom01g04430 pycom01g11810 pycom01g11860 pycom01g11940 pycom01g21380 pycom02g06650 pycom02g08490 pycom02g08600 pycom02g18230 pycom02g18450 pycom02g18800 pycom02g19150 pycom02g20740 pycom02g21340 pycom02g26210 pycom03g12060 pycom03g19060 pycom03g19100 pycom03g19120 pycom04g06890 pycom04g08960 pycom05g01790 pycom05g07910 pycom05g08080 pycom05g08100 pycom05g08570 pycom05g21500 pycom05g22400 pycom05g22510 pycom05g22620 pycom05g23020 pycom05g23720 pycom05g23890 pycom05g24040 pycom07g12080 pycom08g07930 pycom08g11940 pycom09g12120 pycom10g03890 pycom10g23360 pycom10g28250 pycom11g02850 pycom11g14370 pycom11g14400 pycom11g21840 pycom11g21860 pycom11g21980 pycom11g23580 pycom11g23650 pycom11g24050 pycom11g25610 pycom12424g00060 pycom1256g00130 pycom12661g00030 pycom12g09520 pycom12g09530 pycom12g09660 pycom12g09730 pycom12g11210 pycom12g16710 pycom12g16740 pycom12g16750 pycom13g08800 pycom13g18580 pycom13g18670 pycom13g18770 pycom13g22900 pycom13g23050 pycom13g23160 pycom13g23480 pycom13g23610 pycom13g23660 pycom13g23790 pycom13g23840 pycom13g24010 pycom13g24140 pycom13g24210 pycom13g24300 pycom13g24310 pycom13g24480 pycom13g24520 pycom13g24570 pycom13g24730 pycom13g24760 pycom13g24820 pycom13g25010 pycom13g25020 pycom13g25270 pycom13g25620 pycom13g25670 pycom13g25760 pycom13g25800 pycom13g25900 pycom13g26620 pycom13g26640 pycom13g26670 pycom13g26940 pycom13g27040 pycom13g27130 pycom13g27530 pycom13g27770 pycom13g27830 pycom13g27920 pycom13g27940 pycom13g28100 pycom13g28420 pycom13g28430 pycom13g28460 pycom13g28520 pycom13g29160 pycom14g05380 pycom15g03480 pycom15g04370 pycom15g27500 pycom16g13590 pycom16g13610 pycom16g13630 pycom16g14390 pycom16g18310 pycom17g01940 pycom17g04000 pycom17g16100 pycom17g16220 pycom17g16230 pycom17g16340 pycom17g16430 pycom17g16460 pycom17g16740 pycom17g16960 pycom17g17020 pycom17g17090 pycom17g17100 pycom17g17170 pycom17g17430 pycom17g17850 pycom17g18230 pycom17g18250 pycom17g18380 pycom17g21170 pycom17g21330 pycom520g00150 pycom520g00160 pycom808g00030 pycom808g00160 pycom808g00250 pycom808g00290
rosa_chinensis RchiOBHm_Chr5g0054451

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1589
Acc36I ACCTGC 2 cut(s) 697, 845
AccB7I CCANNNNNTGG 2 cut(s) 1879, 2003
AccBSI CCGCTC 1 cut(s) 49
AccI GTMKAC 1 cut(s) 2311
AccII CGCG 8 cut(s) 782, 1059, 1172, 1182, 1206, 1216, 1218, 2403
AciI CCGC 9 cut(s) 29, 47, 75, 293, 782, 788, 831, 1218, 2046
AclI AACGTT 3 cut(s) 1116, 1728, 2325
AclWI GGATC 4 cut(s) 39, 269, 272, 2240
AcoI YGGCCR 5 cut(s) 822, 1219, 1396, 1559, 1994
AcsI RAATTY 2 cut(s) 1554, 1733
AcvI CACGTG 4 cut(s) 290, 760, 805, 1225
AcyI GRCGYC 2 cut(s) 1254, 1586
AfaI GTAC 8 cut(s) 324, 388, 406, 724, 1083, 1779, 1905, 2768
AflIII ACRYGT 2 cut(s) 1084, 2673
AgsI TTSAA 7 cut(s) 539, 1009, 1418, 1653, 1661, 2386, 2719
AhdI GACNNNNNGTC 1 cut(s) 1929
AjiI CACGTC 1 cut(s) 1721
AjnI CCWGG 8 cut(s) 22, 270, 479, 732, 819, 915, 1878, 1996
AluBI AGCT 8 cut(s) 299, 662, 865, 1107, 1618, 1649, 2185, 2648
AluI AGCT 8 cut(s) 299, 662, 865, 1107, 1618, 1649, 2185, 2648
Alw21I GWGCWC 6 cut(s) 289, 664, 839, 867, 1067, 2187
Alw26I GTCTC 4 cut(s) 444, 608, 1818, 2378
Alw44I GTGCAC 2 cut(s) 285, 1063
AlwI GGATC 4 cut(s) 39, 269, 272, 2240
AlwNI CAGNNNCTG 1 cut(s) 2369
Ama87I CYCGRG 4 cut(s) 1811, 2035, 2375, 2816
ApaI GGGCCC 1 cut(s) 1969
ApaLI GTGCAC 2 cut(s) 285, 1063
ApoI RAATTY 2 cut(s) 1554, 1733
AseI ATTAAT 1 cut(s) 2787
AspA2I CCTAGG 1 cut(s) 1621
AspLEI GCGC 4 cut(s) 1174, 1184, 1208, 1218
AsuC2I CCSGG 5 cut(s) 19, 41, 2031, 2036, 2037
AsuII TTCGAA 1 cut(s) 527
AvaI CYCGRG 4 cut(s) 1811, 2035, 2375, 2816
AvaII GGWCC 5 cut(s) 43, 1161, 1198, 1626, 2463
AvrII CCTAGG 1 cut(s) 1621
AxyI CCTNAGG 1 cut(s) 1960
BaeGI GKGCMC 6 cut(s) 289, 491, 820, 1067, 1697, 1969
BalI TGGCCA 1 cut(s) 824
BanII GRGCYC 4 cut(s) 664, 867, 1969, 2187
BauI CACGAG 4 cut(s) 227, 695, 2091, 2198
BbrPI CACGTG 4 cut(s) 290, 760, 805, 1225
BbsI GAAGAC 2 cut(s) 770, 1841
Bbv12I GWGCWC 6 cut(s) 289, 664, 839, 867, 1067, 2187
BccI CCATC 6 cut(s) 60, 153, 236, 1841, 2124, 2207
BceAI ACGGC 2 cut(s) 1241, 1295
BcgI CGANNNNNNTGC 2 cut(s) 266, 300
BciT130I CCWGG 8 cut(s) 24, 272, 481, 734, 821, 917, 1880, 1998
BclI TGATCA 1 cut(s) 2332
BcnI CCSGG 5 cut(s) 19, 41, 2031, 2036, 2037
BcoDI GTCTC 4 cut(s) 444, 608, 1818, 2378
BfaI CTAG 5 cut(s) 161, 233, 1622, 1671, 2204
BfuAI ACCTGC 2 cut(s) 697, 845
BglI GCCNNNNNGGC 1 cut(s) 2351
BisI GCNGC 1 cut(s) 1219
BlnI CCTAGG 1 cut(s) 1621
BlsI GCNGC 1 cut(s) 1220
Bme18I GGWCC 5 cut(s) 43, 1161, 1198, 1626, 2463
BmeRI GACNNNNNGTC 1 cut(s) 1929
BmeT110I CYCGRG 4 cut(s) 1811, 2035, 2375, 2816
BmgBI CACGTC 1 cut(s) 1721
BmiI GGNNCC 9 cut(s) 45, 198, 705, 1581, 1627, 1966, 1967, 2169, 2754
BmrI ACTGGG 4 cut(s) 127, 777, 855, 2098
BmsI GCATC 2 cut(s) 947, 1595
BmuI ACTGGG 4 cut(s) 127, 777, 855, 2098
BoxI GACNNNNGTC 1 cut(s) 1380
BpiI GAAGAC 2 cut(s) 770, 1841
BpmI CTGGAG 1 cut(s) 502
Bpu14I TTCGAA 1 cut(s) 527
BpuEI CTTGAG 1 cut(s) 2819
BpuMI CCSGG 5 cut(s) 19, 41, 2031, 2036, 2037
BsaAI YACGTR 8 cut(s) 290, 760, 805, 1085, 1225, 1457, 1903, 2674
BsaHI GRCGYC 2 cut(s) 1254, 1586
BsaI GGTCTC 2 cut(s) 444, 2378
BsaWI WCCGGW 2 cut(s) 1379, 1386
Bse1I ACTGG 9 cut(s) 133, 207, 772, 850, 1399, 1411, 2104, 2178, 2374
Bse21I CCTNAGG 1 cut(s) 1960
BseBI CCWGG 8 cut(s) 24, 272, 481, 734, 821, 917, 1880, 1998
BseGI GGATG 7 cut(s) 14, 247, 1443, 1934, 2026, 2218, 2261
BseMII CTCAG 1 cut(s) 647
BseNI ACTGG 9 cut(s) 133, 207, 772, 850, 1399, 1411, 2104, 2178, 2374
BseSI GKGCMC 6 cut(s) 289, 491, 820, 1067, 1697, 1969
BsgI GTGCAG 1 cut(s) 873
Bsh1236I CGCG 8 cut(s) 782, 1059, 1172, 1182, 1206, 1216, 1218, 2403
Bsh1285I CGRYCG 1 cut(s) 2407
BsiEI CGRYCG 1 cut(s) 2407
BsiHKAI GWGCWC 6 cut(s) 289, 664, 839, 867, 1067, 2187
BsiHKCI CYCGRG 4 cut(s) 1811, 2035, 2375, 2816
BsiWI CGTACG 4 cut(s) 404, 1081, 1777, 1903
BslFI GGGAC 2 cut(s) 1639, 1936
BsmAI GTCTC 4 cut(s) 444, 608, 1818, 2378
BsmFI GGGAC 2 cut(s) 1639, 1936
Bso31I GGTCTC 2 cut(s) 444, 2378
BsoBI CYCGRG 4 cut(s) 1811, 2035, 2375, 2816
Bsp119I TTCGAA 1 cut(s) 527
Bsp120I GGGCCC 1 cut(s) 1965
Bsp1407I TGTACA 1 cut(s) 2766
Bsp19I CCATGG 1 cut(s) 2539
BspACI CCGC 9 cut(s) 29, 47, 75, 293, 782, 788, 831, 1218, 2046
BspCNI CTCAG 1 cut(s) 648
BspFNI CGCG 8 cut(s) 782, 1059, 1172, 1182, 1206, 1216, 1218, 2403
BspLI GGNNCC 9 cut(s) 45, 198, 705, 1581, 1627, 1966, 1967, 2169, 2754
BspMI ACCTGC 2 cut(s) 697, 845
BspPI GGATC 4 cut(s) 39, 269, 272, 2240
BspT104I TTCGAA 1 cut(s) 527
BspTNI GGTCTC 2 cut(s) 444, 2378
BsrBI CCGCTC 1 cut(s) 49
BsrGI TGTACA 1 cut(s) 2766
BsrI ACTGG 9 cut(s) 133, 207, 772, 850, 1399, 1411, 2104, 2178, 2374
BssNAI GTATAC 1 cut(s) 2312
BssNI GRCGYC 2 cut(s) 1254, 1586
BssSI CACGAG 4 cut(s) 227, 695, 2091, 2198
BssT1I CCWWGG 3 cut(s) 1621, 2241, 2539
Bst1107I GTATAC 1 cut(s) 2312
Bst2BI CACGAG 4 cut(s) 227, 695, 2091, 2198
Bst2UI CCWGG 8 cut(s) 24, 272, 481, 734, 821, 917, 1880, 1998
Bst6I CTCTTC 2 cut(s) 173, 2144
BstACI GRCGYC 2 cut(s) 1254, 1586
BstAUI TGTACA 1 cut(s) 2766
BstBAI YACGTR 8 cut(s) 290, 760, 805, 1085, 1225, 1457, 1903, 2674
BstBI TTCGAA 1 cut(s) 527
BstC8I GCNNGC 2 cut(s) 1067, 1364
BstDEI CTNAG 5 cut(s) 656, 1960, 2286, 2534, 2799
BstDSI CCRYGG 3 cut(s) 825, 1525, 2539
BstEII GGTNACC 4 cut(s) 139, 398, 1382, 2110
BstF5I GGATG 7 cut(s) 14, 247, 1443, 1934, 2026, 2218, 2261
BstFNI CGCG 8 cut(s) 782, 1059, 1172, 1182, 1206, 1216, 1218, 2403
BstHHI GCGC 4 cut(s) 1174, 1184, 1208, 1218
BstMAI GTCTC 4 cut(s) 444, 608, 1818, 2378
BstMCI CGRYCG 1 cut(s) 2407
BstMWI GCNNNNNNNGC 5 cut(s) 577, 917, 938, 2351, 2654
BstNI CCWGG 8 cut(s) 24, 272, 481, 734, 821, 917, 1880, 1998
BstPAI GACNNNNGTC 1 cut(s) 1380
BstPI GGTNACC 4 cut(s) 139, 398, 1382, 2110
BstSLI GKGCMC 6 cut(s) 289, 491, 820, 1067, 1697, 1969
BstUI CGCG 8 cut(s) 782, 1059, 1172, 1182, 1206, 1216, 1218, 2403
BstV2I GAAGAC 2 cut(s) 770, 1841
BstZ17I GTATAC 1 cut(s) 2312
Bsu36I CCTNAGG 1 cut(s) 1960
BtgI CCRYGG 3 cut(s) 825, 1525, 2539
BtgZI GCGATG 1 cut(s) 2262
BtrI CACGTC 1 cut(s) 1721
BtsCI GGATG 7 cut(s) 14, 247, 1443, 1934, 2026, 2218, 2261
BtsI GCAGTG 2 cut(s) 1858, 2735
BveI ACCTGC 2 cut(s) 697, 845
Cac8I GCNNGC 2 cut(s) 1067, 1364
CaiI CAGNNNCTG 1 cut(s) 2369
CfoI GCGC 4 cut(s) 1174, 1184, 1208, 1218
Cfr9I CCCGGG 1 cut(s) 2035
CseI GACGC 2 cut(s) 921, 1262
Csp6I GTAC 8 cut(s) 323, 387, 405, 723, 1082, 1778, 1904, 2767
CviQI GTAC 8 cut(s) 323, 387, 405, 723, 1082, 1778, 1904, 2767
DdeI CTNAG 5 cut(s) 656, 1960, 2286, 2534, 2799
DraI TTTAAA 1 cut(s) 1126
DriI GACNNNNNGTC 1 cut(s) 1929
EaeI YGGCCR 5 cut(s) 822, 1219, 1396, 1559, 1994
Eam1104I CTCTTC 2 cut(s) 173, 2144
Eam1105I GACNNNNNGTC 1 cut(s) 1929
EarI CTCTTC 2 cut(s) 173, 2144
EciI GGCGGA 1 cut(s) 44
Ecl136II GAGCTC 3 cut(s) 662, 865, 2185
Eco130I CCWWGG 3 cut(s) 1621, 2241, 2539
Eco147I AGGCCT 2 cut(s) 732, 2196
Eco24I GRGCYC 4 cut(s) 664, 867, 1969, 2187
Eco31I GGTCTC 2 cut(s) 444, 2378
Eco47I GGWCC 5 cut(s) 43, 1161, 1198, 1626, 2463
Eco53kI GAGCTC 3 cut(s) 662, 865, 2185
Eco72I CACGTG 4 cut(s) 290, 760, 805, 1225
Eco81I CCTNAGG 1 cut(s) 1960
Eco88I CYCGRG 4 cut(s) 1811, 2035, 2375, 2816
Eco91I GGTNACC 4 cut(s) 139, 398, 1382, 2110
EcoICRI GAGCTC 3 cut(s) 662, 865, 2185
EcoO109I RGGNCCY 4 cut(s) 1514, 1626, 1965, 2463
EcoO65I GGTNACC 4 cut(s) 139, 398, 1382, 2110
EcoRII CCWGG 8 cut(s) 22, 270, 479, 732, 819, 915, 1878, 1996
EcoT14I CCWWGG 3 cut(s) 1621, 2241, 2539
EcoT22I ATGCAT 1 cut(s) 984
EcoT38I GRGCYC 4 cut(s) 664, 867, 1969, 2187
ErhI CCWWGG 3 cut(s) 1621, 2241, 2539
FalI AAGNNNNNCTT 4 cut(s) 250, 282, 2221, 2253
FaqI GGGAC 2 cut(s) 1639, 1936
FauI CCCGC 4 cut(s) 54, 775, 781, 824
FauNDI CATATG 1 cut(s) 557
FbaI TGATCA 1 cut(s) 2332
FblI GTMKAC 1 cut(s) 2311
Fnu4HI GCNGC 1 cut(s) 1219
FokI GGATG 6 cut(s) 254, 1430, 1941, 2013, 2225, 2268
FriOI GRGCYC 4 cut(s) 664, 867, 1969, 2187
Fsp4HI GCNGC 1 cut(s) 1219
FspBI CTAG 5 cut(s) 161, 233, 1622, 1671, 2204
GlaI GCGC 4 cut(s) 1173, 1183, 1207, 1217
GluI GCNGC 1 cut(s) 1219
GsuI CTGGAG 1 cut(s) 502
HgaI GACGC 2 cut(s) 921, 1262
HhaI GCGC 4 cut(s) 1174, 1184, 1208, 1218
Hin1I GRCGYC 2 cut(s) 1254, 1586
Hin6I GCGC 4 cut(s) 1172, 1182, 1206, 1216
HinP1I GCGC 4 cut(s) 1172, 1182, 1206, 1216
HincII GTYRAC 1 cut(s) 1120
HindII GTYRAC 1 cut(s) 1120
HindIII AAGCTT 1 cut(s) 1647
HinfI GANTC 9 cut(s) 439, 698, 1376, 1530, 1809, 2378, 2390, 2572, 2604
HpaI GTTAAC 1 cut(s) 1120
Hpy188I TCNGA 7 cut(s) 185, 345, 426, 657, 703, 1584, 2156
Hpy188III TCNNGA 7 cut(s) 434, 1261, 1282, 1921, 2091, 2330, 2375
Hpy99I CGWCG 4 cut(s) 957, 1588, 1722, 1985
HpyAV CCTTC 5 cut(s) 389, 802, 1029, 1443, 1792
HpyF10VI GCNNNNNNNGC 5 cut(s) 577, 917, 938, 2351, 2654
HpyF3I CTNAG 5 cut(s) 656, 1960, 2286, 2534, 2799
Hsp92I GRCGYC 2 cut(s) 1254, 1586
HspAI GCGC 4 cut(s) 1172, 1182, 1206, 1216
Ksp22I TGATCA 1 cut(s) 2332
KspAI GTTAAC 1 cut(s) 1120
LmnI GCTCC 4 cut(s) 54, 834, 1623, 2190
LweI GCATC 2 cut(s) 947, 1595
MaeI CTAG 5 cut(s) 161, 233, 1622, 1671, 2204
MbiI CCGCTC 1 cut(s) 49
MboII GAAGA 9 cut(s) 160, 775, 1345, 1801, 1804, 1807, 1846, 2131, 2731
MfeI CAATTG 3 cut(s) 1248, 2347, 2709
MlsI TGGCCA 1 cut(s) 824
MluNI TGGCCA 1 cut(s) 824
MlyI GAGTC 5 cut(s) 707, 1370, 1803, 2387, 2399
MmeI TCCRAC 4 cut(s) 681, 1480, 1607, 1624
Mox20I TGGCCA 1 cut(s) 824
Mph1103I ATGCAT 1 cut(s) 984
MscI TGGCCA 1 cut(s) 824
MslI CAYNNNNRTG 4 cut(s) 598, 900, 1094, 2426
Msp20I TGGCCA 1 cut(s) 824
MunI CAATTG 3 cut(s) 1248, 2347, 2709
MvaI CCWGG 8 cut(s) 24, 272, 481, 734, 821, 917, 1880, 1998
MvnI CGCG 8 cut(s) 782, 1059, 1172, 1182, 1206, 1216, 1218, 2403
MwoI GCNNNNNNNGC 5 cut(s) 577, 917, 938, 2351, 2654
NciI CCSGG 5 cut(s) 19, 41, 2031, 2036, 2037
NcoI CCATGG 1 cut(s) 2539
NdeI CATATG 1 cut(s) 557
NlaIV GGNNCC 9 cut(s) 45, 198, 705, 1581, 1627, 1966, 1967, 2169, 2754
NmeAIII GCCGAG 1 cut(s) 1056
NsiI ATGCAT 1 cut(s) 984
NspV TTCGAA 1 cut(s) 527
PacI TTAATTAA 1 cut(s) 2787
PaeR7I CTCGAG 1 cut(s) 2375
PasI CCCWGGG 1 cut(s) 271
PceI AGGCCT 2 cut(s) 732, 2196
PcsI WCGNNNNNNNCGW 2 cut(s) 1077, 1908
PfeI GAWTC 4 cut(s) 439, 1530, 2572, 2604
Pfl23II CGTACG 4 cut(s) 404, 1081, 1777, 1903
PflFI GACNNNGTC 3 cut(s) 1243, 1256, 2480
PflMI CCANNNNNTGG 2 cut(s) 1879, 2003
PfoI TCCNGGA 1 cut(s) 39
PkrI GCNGC 1 cut(s) 1220
Ple19I CGATCG 1 cut(s) 2407
PleI GAGTC 5 cut(s) 706, 1370, 1803, 2386, 2398
PmaCI CACGTG 4 cut(s) 290, 760, 805, 1225
PmlI CACGTG 4 cut(s) 290, 760, 805, 1225
PpsI GAGTC 5 cut(s) 706, 1370, 1803, 2386, 2398
Ppu21I YACGTR 8 cut(s) 290, 760, 805, 1085, 1225, 1457, 1903, 2674
PpuMI RGGWCCY 2 cut(s) 1626, 2463
PshAI GACNNNNGTC 1 cut(s) 1380
PshBI ATTAAT 1 cut(s) 2787
Psp124BI GAGCTC 3 cut(s) 664, 867, 2187
Psp1406I AACGTT 3 cut(s) 1116, 1728, 2325
Psp5II RGGWCCY 2 cut(s) 1626, 2463
Psp6I CCWGG 8 cut(s) 22, 270, 479, 732, 819, 915, 1878, 1996
PspCI CACGTG 4 cut(s) 290, 760, 805, 1225
PspEI GGTNACC 4 cut(s) 139, 398, 1382, 2110
PspGI CCWGG 8 cut(s) 22, 270, 479, 732, 819, 915, 1878, 1996
PspLI CGTACG 4 cut(s) 404, 1081, 1777, 1903
PspN4I GGNNCC 9 cut(s) 45, 198, 705, 1581, 1627, 1966, 1967, 2169, 2754
PspOMI GGGCCC 1 cut(s) 1965
PspPPI RGGWCCY 2 cut(s) 1626, 2463
PstNI CAGNNNCTG 1 cut(s) 2369
PsyI GACNNNGTC 3 cut(s) 1243, 1256, 2480
PvuI CGATCG 1 cut(s) 2407
RsaI GTAC 8 cut(s) 324, 388, 406, 724, 1083, 1779, 1905, 2768
RsaNI GTAC 8 cut(s) 323, 387, 405, 723, 1082, 1778, 1904, 2767
RseI CAYNNNNRTG 4 cut(s) 598, 900, 1094, 2426
SacI GAGCTC 3 cut(s) 664, 867, 2187
SatI GCNGC 1 cut(s) 1219
SchI GAGTC 5 cut(s) 707, 1370, 1803, 2387, 2399
SfaNI GCATC 2 cut(s) 947, 1595
Sfr274I CTCGAG 1 cut(s) 2375
SfuI TTCGAA 1 cut(s) 527
SinI GGWCC 5 cut(s) 43, 1161, 1198, 1626, 2463
SlaI CTCGAG 1 cut(s) 2375
SmaI CCCGGG 1 cut(s) 2037
SmiMI CAYNNNNRTG 4 cut(s) 598, 900, 1094, 2426
SmlI CTYRAG 2 cut(s) 2375, 2834
SmoI CTYRAG 2 cut(s) 2375, 2834
SseBI AGGCCT 2 cut(s) 732, 2196
SsiI CCGC 9 cut(s) 29, 47, 75, 293, 782, 788, 831, 1218, 2046
SspI AATATT 6 cut(s) 949, 1267, 1288, 1321, 1343, 2221
SspMI CTAG 5 cut(s) 161, 233, 1622, 1671, 2204
SstI GAGCTC 3 cut(s) 664, 867, 2187
StuI AGGCCT 2 cut(s) 732, 2196
StyI CCWWGG 3 cut(s) 1621, 2241, 2539
TaqI TCGA 7 cut(s) 527, 952, 1712, 1741, 1911, 2376, 2407
TatI WGTACW 2 cut(s) 386, 2766
TauI GCSGC 1 cut(s) 1221
TfiI GAWTC 4 cut(s) 439, 1530, 2572, 2604
TspDTI ATGAA 7 cut(s) 1432, 1488, 1565, 1701, 2213, 2451, 2805
TspMI CCCGGG 1 cut(s) 2035
Tth111I GACNNNGTC 3 cut(s) 1243, 1256, 2480
Van91I CCANNNNNTGG 2 cut(s) 1879, 2003
VneI GTGCAC 2 cut(s) 285, 1063
VpaK11BI GGWCC 5 cut(s) 43, 1161, 1198, 1626, 2463
VspI ATTAAT 1 cut(s) 2787
XapI RAATTY 2 cut(s) 1554, 1733
XcmI CCANNNNNNNNNTGG 1 cut(s) 2516
XhoI CTCGAG 1 cut(s) 2375
XmaI CCCGGG 1 cut(s) 2035
XmaJI CCTAGG 1 cut(s) 1621
XmiI GTMKAC 1 cut(s) 2311
XspI CTAG 5 cut(s) 161, 233, 1622, 1671, 2204
ZraI GACGTC 1 cut(s) 1587
Zsp2I ATGCAT 1 cut(s) 984
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.