pycom05g08080

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000158
Physical Location & Seq
Forward (+)
10821709 .. 10825246
3538 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g08080.1

Sequence Viewer

Length: 2547 bp
ATGTGGTCTGCACGACAAGGCTTGCACCTAACTTGGATCCAAGGCGAGCGTGTGGTGCTGGTGAACATACACGTGAAGGACTCTGCCCCTCTCTGGGCGGGAGCACTAACACCGGGGGTGCAGATTATGAGAATGCATAACCACATGAATACCGCTTACCTGGCACTTACCTGTGCGTCCACAGCACCAAATACACATTTATATATATGTATACCACTACTAATACATGCGATGATGCGTAAACAACATTCATACGATGCATGGCATAAAACAAATAACCTTTTTTATTCTATTTCTGGGAATTTAGATGTATATAGGTATATACGGAAAACAAAAGCCCACTCACTGATAATTAGAAGGGTCGTAGCCCCCCTGCCTCGAGTGTGTACGCTCGTCCTCGGACACCTAAGCAACTTCTCATACCAACTTTTGGACCACGCACGCGCCCCCACGCGCCTGAACAGGCACGGACCTACGCGCCCCCACGCGCGGCCACGTGCACTGCACACTGACGGTGTCAACAGACGCCGTCAGGAATATTCTGTCAAATTGACGGAATATTCCGTTAAATCTAACCAGATTCCGTCACTGCCGTTAGGAATATTCCTCTCCGGCGCCGGCGACGGCGCCGGAAAACTGGGAATTTTTCAAACTTTGTTTTCTCCTTCGTTTTTCAACCAAATTTCACGTTCTTTATACCAAAATGAACACACGATCATACCTGCAACAACGATCCAAGTTCGGCCAACTTTGAACAGGACGATCCCGACGTCCAAACTCACCCAACGAACCACTCCGAGGCTCCTTGGGACCTCACAAACACATCTACAATGTTCAAATCGGACCTCGTCGAAACGACGTGAAAACGTCAAAGTTCTTACCTGGAATTGGCACCACTGGACTCCTCTCAGCCTCACGATCCGTATGTTGAGAAGAGAAGGAGAAGAAGATGAACTCGGGGAGGAAGAGAGAGAGAGAGAGAGAGAGAAAAAAAGAAAGGGTATGAGTGTGGGTGGTCCTACACACCACACCATACAACACAAAACTACCCGTAAGTTTAACGAACTAAGGGGTAAAATCGTAAATTCACACGTACGTTTTAATAATCCCGGGACGGGATGTTACACCCTTAAAATTATGAGTATGAAGACGGAAATGATAATGGCATACCCATCACTGTTAACTATTATCCCCCTTATCCTCTTAATTGGTAAAGATACCTTGACTTTATACATTTGGTCAGGATACTTTTTTTTAGGAGAATATATGCATGATGCATATATACAATTTTGTTTTTATAATATAATAAGTTATTTTGGTATATGTCACTCCAAAAATCTCATTCTACACTCCAAACTTTCTATATTAAGAAAAAAAAATACATTTGTGAGGAGTAAGATATATTATCAAGATAGTATAGTGCTACTTTTTAATGGAACATTATTGTGCATTTTTTTTTCTCAGCAATTGAAAGTTTTATTTTATTTTATTTTATTTTTTTTAGAGGTCTTAGGTTCGGTTCTTACCAAAGGTAAATTTGAAACATATTATCACTACCTTATATGTATATTCCCATCTCTACCTAGAACGAGGCCTTTTGGGAGCTCACTGGCTTCAGGTTTCATGGGAACTCAGAAGTTAAGCGAGTGGCACGCGAGAGCATTTCCAGGATGGGTGACCCATCGGGAAGTTCTCCCCAAAGCGGACAATATCGTGCTACGGTGGAGTCGGGCTTGGGAAGTGGTCCCGCTCGGGCCGGGAAGTGACAATTTGGTATCAGAGCCTAACCTTGGCCTCGTGTGTGCCGACGAGGACGTCGGGCCCCTAAGGGGGTGGATTGTGACACCCTACATCCACGAGGCCTTTTGGGATCTCACTGGCTTCGGGTTCCATGGGAACTCCGAAGTTAAGCGAGTAGCATGCGAGAGCATTTCCAGGATGGGTGACCCATCGGGAAGTTCTCGTGTGAGTTCCCAGAAAGAAAACCATGAGGGTGTGCTGGGGGCCCAAGTGGTCCCACCCGGGCCGAGATGTGATAATTTGGTATCAGAGCCTAACCTTGGCCGCGTGTGTGTCGACAAGGACGTCGGGCCCCTAAGGGGGGTGGATTGTGACATCCCACATCGCCCAGGGGATCGATCCTTATATCTCACTGGCGTCGGGTTCCATGGGAACTCCGAAGTTAAGCGAGTAGCACCCGAGAGCATTTCTAGGATGGGTGACCCATCGGGAAGTTCTCGTGTGAGTTCCCAGAAAGAAAACCGTGAGGGTGTGCTAGGGGCCCAAAGTGGACAATATTGTGTTACGGTGGAGTCGGTCACTTTTCTTTCTTTTAGTGTAAATAATATCACAAAGGAGGTTCAAAAGTGGAAGAAAGGAGGGGGTGGTCACCTACCGCCACGCCTAAATGGCAAGTTCATCCCAATCTCACCAACTTATTTTTGGAGTTGGAATGATAGTGGTCCCCTTCTAAAAAAAAAGGTGGTGATGCATGATCATGTCCGATTTCTTTTGGTAAATAAAAATATACGGTTAAAATTACGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

849

Amino Acids

96.2

Weight (kDa)

9.83

Isoelectric Point (pI)

40.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000158)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10163
malus_domestica MD04G1068000.v1.1 MD05G1130900.v1.1 MD06G1153600.v1.1 MD10G1255900.v1.1 MD10G1282000.v1.1 MD13G1075400.v1.1 MD13G1192400.v1.1
pyrus_communis pycom01g00130 pycom01g00390 pycom01g00700 pycom01g00720 pycom01g00750 pycom01g00850 pycom01g00870 pycom01g00910 pycom01g01250 pycom01g01560 pycom01g01680 pycom01g01860 pycom01g01900 pycom01g01910 pycom01g02200 pycom01g02260 pycom01g02270 pycom01g02280 pycom01g02330 pycom01g02480 pycom01g02720 pycom01g02730 pycom01g02750 pycom01g02770 pycom01g02780 pycom01g02860 pycom01g02910 pycom01g03540 pycom01g04090 pycom01g04100 pycom01g04150 pycom01g04250 pycom01g04430 pycom01g11810 pycom01g11860 pycom01g11940 pycom01g21380 pycom02g06650 pycom02g08490 pycom02g08600 pycom02g18230 pycom02g18450 pycom02g18800 pycom02g19150 pycom02g20740 pycom02g21340 pycom02g26210 pycom03g12060 pycom03g19060 pycom03g19100 pycom03g19120 pycom04g06890 pycom04g08960 pycom05g01790 pycom05g07910 pycom05g08080 pycom05g08100 pycom05g08570 pycom05g21500 pycom05g22400 pycom05g22510 pycom05g22620 pycom05g23020 pycom05g23720 pycom05g23890 pycom05g24040 pycom07g12080 pycom08g07930 pycom08g11940 pycom09g12120 pycom10g03890 pycom10g23360 pycom10g28250 pycom11g02850 pycom11g14370 pycom11g14400 pycom11g21840 pycom11g21860 pycom11g21980 pycom11g23580 pycom11g23650 pycom11g24050 pycom11g25610 pycom12424g00060 pycom1256g00130 pycom12661g00030 pycom12g09520 pycom12g09530 pycom12g09660 pycom12g09730 pycom12g11210 pycom12g16710 pycom12g16740 pycom12g16750 pycom13g08800 pycom13g18580 pycom13g18670 pycom13g18770 pycom13g22900 pycom13g23050 pycom13g23160 pycom13g23480 pycom13g23610 pycom13g23660 pycom13g23790 pycom13g23840 pycom13g24010 pycom13g24140 pycom13g24210 pycom13g24300 pycom13g24310 pycom13g24480 pycom13g24520 pycom13g24570 pycom13g24730 pycom13g24760 pycom13g24820 pycom13g25010 pycom13g25020 pycom13g25270 pycom13g25620 pycom13g25670 pycom13g25760 pycom13g25800 pycom13g25900 pycom13g26620 pycom13g26640 pycom13g26670 pycom13g26940 pycom13g27040 pycom13g27130 pycom13g27530 pycom13g27770 pycom13g27830 pycom13g27920 pycom13g27940 pycom13g28100 pycom13g28420 pycom13g28430 pycom13g28460 pycom13g28520 pycom13g29160 pycom14g05380 pycom15g03480 pycom15g04370 pycom15g27500 pycom16g13590 pycom16g13610 pycom16g13630 pycom16g14390 pycom16g18310 pycom17g01940 pycom17g04000 pycom17g16100 pycom17g16220 pycom17g16230 pycom17g16340 pycom17g16430 pycom17g16460 pycom17g16740 pycom17g16960 pycom17g17020 pycom17g17090 pycom17g17100 pycom17g17170 pycom17g17430 pycom17g17850 pycom17g18230 pycom17g18250 pycom17g18380 pycom17g21170 pycom17g21330 pycom520g00150 pycom520g00160 pycom808g00030 pycom808g00160 pycom808g00250 pycom808g00290
rosa_chinensis RchiOBHm_Chr5g0054451

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1299
AasI GACNNNNNNGTC 2 cut(s) 1814, 2084
AatII GACGTC 3 cut(s) 773, 1818, 2088
Acc36I ACCTGC 1 cut(s) 730
AccB1I GGYRCC 3 cut(s) 614, 626, 891
AccB7I CCANNNNNTGG 1 cut(s) 430
AccBSI CCGCTC 1 cut(s) 1750
AccI GTMKAC 2 cut(s) 211, 2076
AccII CGCG 7 cut(s) 444, 454, 478, 488, 490, 1655, 2067
AciI CCGC 7 cut(s) 98, 153, 490, 1703, 1748, 2065, 2396
AclWI GGATC 8 cut(s) 31, 44, 727, 757, 913, 1878, 2133, 2142
AcoI YGGCCR 3 cut(s) 491, 743, 2062
AcsI RAATTY 5 cut(s) 301, 642, 681, 1084, 1535
AcuI CTGAAG 1 cut(s) 1599
AcvI CACGTG 2 cut(s) 73, 497
AcyI GRCGYC 7 cut(s) 526, 615, 627, 770, 1815, 2085, 2157
AfaI GTAC 2 cut(s) 388, 1095
AflIII ACRYGT 2 cut(s) 70, 1090
AgsI TTSAA 7 cut(s) 650, 676, 754, 837, 1471, 1541, 2363
AjiI CACGTC 1 cut(s) 860
AjnI CCWGG 5 cut(s) 159, 881, 1666, 1934, 2128
AluBI AGCT 1 cut(s) 1605
AluI AGCT 1 cut(s) 1605
Alw21I GWGCWC 3 cut(s) 106, 502, 1607
Alw44I GTGCAC 1 cut(s) 498
AlwI GGATC 8 cut(s) 31, 44, 727, 757, 913, 1878, 2133, 2142
Ama87I CYCGRG 6 cut(s) 378, 956, 1109, 1751, 2021, 2198
ApaI GGGCCC 4 cut(s) 1824, 2008, 2094, 2284
ApaLI GTGCAC 1 cut(s) 498
ApoI RAATTY 5 cut(s) 301, 642, 681, 1084, 1535
AspLEI GCGC 6 cut(s) 446, 456, 480, 490, 617, 629
AsuC2I CCSGG 6 cut(s) 114, 1110, 1111, 1758, 2022, 2023
AsuHPI GGTGA 8 cut(s) 73, 772, 1687, 1955, 2231, 2381, 2421, 2497
AvaI CYCGRG 6 cut(s) 378, 956, 1109, 1751, 2021, 2198
AvaII GGWCC 8 cut(s) 433, 470, 810, 843, 1016, 1744, 2014, 2462
AxyI CCTNAGG 2 cut(s) 1826, 2096
BaeGI GKGCMC 5 cut(s) 502, 1824, 2008, 2094, 2284
BamHI GGATCC 1 cut(s) 36
BanI GGYRCC 3 cut(s) 614, 626, 891
BanII GRGCYC 5 cut(s) 1607, 1824, 2008, 2094, 2284
BauI CACGAG 4 cut(s) 1796, 1856, 1962, 2238
BbrPI CACGTG 2 cut(s) 73, 497
BbsI GAAGAC 1 cut(s) 1154
Bbv12I GWGCWC 3 cut(s) 106, 502, 1607
BccI CCATC 8 cut(s) 1180, 1582, 1665, 1689, 1933, 1957, 2209, 2233
BceAI ACGGC 3 cut(s) 513, 577, 640
BcgI CGANNNNNNTGC 2 cut(s) 1902, 1936
BciT130I CCWGG 5 cut(s) 161, 883, 1668, 1936, 2130
BciVI GTATCC 1 cut(s) 1238
BclI TGATCA 1 cut(s) 2494
BcnI CCSGG 6 cut(s) 114, 1110, 1111, 1758, 2022, 2023
BfaI CTAG 3 cut(s) 1584, 2211, 2276
BfoI RGCGCY 2 cut(s) 618, 630
BfuAI ACCTGC 1 cut(s) 730
BfuI GTATCC 1 cut(s) 1238
BglI GCCNNNNNGGC 1 cut(s) 2409
BisI GCNGC 2 cut(s) 491, 2065
BlsI GCNGC 2 cut(s) 492, 2066
Bme18I GGWCC 8 cut(s) 433, 470, 810, 843, 1016, 1744, 2014, 2462
BmeT110I CYCGRG 6 cut(s) 378, 956, 1109, 1751, 2021, 2198
BmgBI CACGTC 1 cut(s) 860
BmrI ACTGGG 1 cut(s) 647
BmsI GCATC 4 cut(s) 225, 247, 1264, 2478
BmuI ACTGGG 1 cut(s) 647
BpiI GAAGAC 1 cut(s) 1154
Bpu10I CCTNAGC 1 cut(s) 407
BpuMI CCSGG 6 cut(s) 114, 1110, 1111, 1758, 2022, 2023
Bsa29I ATCGAT 1 cut(s) 2137
BsaAI YACGTR 3 cut(s) 73, 497, 1093
BsaHI GRCGYC 7 cut(s) 526, 615, 627, 770, 1815, 2085, 2157
Bse118I RCCGGY 1 cut(s) 617
Bse1I ACTGG 5 cut(s) 642, 902, 1614, 1882, 2158
Bse21I CCTNAGG 2 cut(s) 1826, 2096
BseBI CCWGG 5 cut(s) 161, 883, 1668, 1936, 2130
BseCI ATCGAT 1 cut(s) 2137
BseGI GGATG 7 cut(s) 1124, 1676, 1851, 1944, 2115, 2220, 2418
BseMII CTCAG 3 cut(s) 922, 1475, 1646
BseNI ACTGG 5 cut(s) 642, 902, 1614, 1882, 2158
BseRI GAGGAG 2 cut(s) 894, 1405
BseSI GKGCMC 5 cut(s) 502, 1824, 2008, 2094, 2284
BseYI CCCAGC 1 cut(s) 1999
BsgI GTGCAG 2 cut(s) 140, 488
Bsh1236I CGCG 7 cut(s) 444, 454, 478, 488, 490, 1655, 2067
BshNI GGYRCC 3 cut(s) 614, 626, 891
BshVI ATCGAT 1 cut(s) 2137
BsiHKAI GWGCWC 3 cut(s) 106, 502, 1607
BsiHKCI CYCGRG 6 cut(s) 378, 956, 1109, 1751, 2021, 2198
BsiSI CCGG 7 cut(s) 113, 612, 618, 630, 1110, 1757, 2022
BsiWI CGTACG 1 cut(s) 1093
BslFI GGGAC 5 cut(s) 823, 1126, 1730, 2000, 2448
BsmFI GGGAC 5 cut(s) 823, 1126, 1730, 2000, 2448
BsmI GAATGC 1 cut(s) 138
BsoBI CYCGRG 6 cut(s) 378, 956, 1109, 1751, 2021, 2198
Bsp120I GGGCCC 4 cut(s) 1820, 2004, 2090, 2280
Bsp1286I GDGCHC 7 cut(s) 106, 502, 1607, 1824, 2008, 2094, 2284
Bsp143I GATC 9 cut(s) 36, 714, 732, 762, 918, 1870, 2134, 2138, 2494
Bsp19I CCATGG 2 cut(s) 1891, 2167
BspACI CCGC 7 cut(s) 98, 153, 490, 1703, 1748, 2065, 2396
BspCNI CTCAG 3 cut(s) 921, 1474, 1645
BspDI ATCGAT 1 cut(s) 2137
BspFNI CGCG 7 cut(s) 444, 454, 478, 488, 490, 1655, 2067
BspMI ACCTGC 1 cut(s) 730
BspPI GGATC 8 cut(s) 31, 44, 727, 757, 913, 1878, 2133, 2142
BspT107I GGYRCC 3 cut(s) 614, 626, 891
BsrBI CCGCTC 1 cut(s) 1750
BsrFI RCCGGY 1 cut(s) 617
BsrI ACTGG 5 cut(s) 642, 902, 1614, 1882, 2158
BssAI RCCGGY 1 cut(s) 617
BssMI GATC 9 cut(s) 36, 714, 732, 762, 918, 1870, 2134, 2138, 2494
BssNAI GTATAC 1 cut(s) 212
BssNI GRCGYC 7 cut(s) 526, 615, 627, 770, 1815, 2085, 2157
BssSI CACGAG 4 cut(s) 1796, 1856, 1962, 2238
BssT1I CCWWGG 6 cut(s) 40, 805, 1789, 1891, 2059, 2167
Bst1107I GTATAC 1 cut(s) 212
Bst2BI CACGAG 4 cut(s) 1796, 1856, 1962, 2238
Bst2UI CCWGG 5 cut(s) 161, 883, 1668, 1936, 2130
Bst4CI ACNGT 6 cut(s) 515, 1179, 1722, 2264, 2308, 2532
Bst6I CTCTTC 2 cut(s) 928, 960
BstACI GRCGYC 7 cut(s) 526, 615, 627, 770, 1815, 2085, 2157
BstBAI YACGTR 3 cut(s) 73, 497, 1093
BstC8I GCNNGC 6 cut(s) 23, 47, 442, 619, 1653, 1921
BstDEI CTNAG 8 cut(s) 407, 908, 1067, 1461, 1510, 1632, 1826, 2096
BstDSI CCRYGG 2 cut(s) 1891, 2167
BstEII GGTNACC 4 cut(s) 1675, 1943, 2219, 2387
BstF5I GGATG 7 cut(s) 1124, 1676, 1851, 1944, 2115, 2220, 2418
BstFNI CGCG 7 cut(s) 444, 454, 478, 488, 490, 1655, 2067
BstH2I RGCGCY 2 cut(s) 618, 630
BstHHI GCGC 6 cut(s) 446, 456, 480, 490, 617, 629
BstKTI GATC 9 cut(s) 39, 717, 735, 765, 921, 1873, 2137, 2141, 2497
BstMBI GATC 9 cut(s) 36, 714, 732, 762, 918, 1870, 2134, 2138, 2494
BstMWI GCNNNNNNNGC 4 cut(s) 55, 161, 182, 2409
BstNI CCWGG 5 cut(s) 161, 883, 1668, 1936, 2130
BstNSI RCATGY 2 cut(s) 230, 1923
BstPI GGTNACC 4 cut(s) 1675, 1943, 2219, 2387
BstSLI GKGCMC 5 cut(s) 502, 1824, 2008, 2094, 2284
BstUI CGCG 7 cut(s) 444, 454, 478, 488, 490, 1655, 2067
BstV2I GAAGAC 1 cut(s) 1154
BstX2I RGATCY 2 cut(s) 36, 1870
BstYI RGATCY 2 cut(s) 36, 1870
BstZ17I GTATAC 1 cut(s) 212
Bsu15I ATCGAT 1 cut(s) 2137
Bsu36I CCTNAGG 2 cut(s) 1826, 2096
BsuI GTATCC 1 cut(s) 1238
BsuTUI ATCGAT 1 cut(s) 2137
BtgI CCRYGG 2 cut(s) 1891, 2167
BtgZI GCGATG 2 cut(s) 245, 2108
BtrI CACGTC 1 cut(s) 860
BtsCI GGATG 7 cut(s) 1124, 1676, 1851, 1944, 2115, 2220, 2418
BtsI GCAGTG 2 cut(s) 500, 587
BtsIMutI CAGTG 9 cut(s) 344, 500, 507, 587, 895, 1175, 1607, 1875, 2151
BveI ACCTGC 1 cut(s) 730
Cac8I GCNNGC 6 cut(s) 23, 47, 442, 619, 1653, 1921
CfoI GCGC 6 cut(s) 446, 456, 480, 490, 617, 629
Cfr10I RCCGGY 1 cut(s) 617
Cfr9I CCCGGG 2 cut(s) 1109, 2021
ClaI ATCGAT 1 cut(s) 2137
CseI GACGC 3 cut(s) 165, 534, 2146
Csp6I GTAC 2 cut(s) 387, 1094
CviQI GTAC 2 cut(s) 387, 1094
DdeI CTNAG 8 cut(s) 407, 908, 1067, 1461, 1510, 1632, 1826, 2096
DinI GGCGCC 2 cut(s) 616, 628
DpnI GATC 9 cut(s) 38, 716, 734, 764, 920, 1872, 2136, 2140, 2496
DpnII GATC 9 cut(s) 36, 714, 732, 762, 918, 1870, 2134, 2138, 2494
DrdI GACNNNNNNGTC 2 cut(s) 1814, 2084
DseDI GACNNNNNNGTC 2 cut(s) 1814, 2084
EaeI YGGCCR 3 cut(s) 491, 743, 2062
Eam1104I CTCTTC 2 cut(s) 928, 960
EarI CTCTTC 2 cut(s) 928, 960
Ecl136II GAGCTC 1 cut(s) 1605
Eco130I CCWWGG 6 cut(s) 40, 805, 1789, 1891, 2059, 2167
Eco147I AGGCCT 2 cut(s) 1594, 1862
Eco24I GRGCYC 5 cut(s) 1607, 1824, 2008, 2094, 2284
Eco47I GGWCC 8 cut(s) 433, 470, 810, 843, 1016, 1744, 2014, 2462
Eco53kI GAGCTC 1 cut(s) 1605
Eco57I CTGAAG 1 cut(s) 1599
Eco72I CACGTG 2 cut(s) 73, 497
Eco81I CCTNAGG 2 cut(s) 1826, 2096
Eco88I CYCGRG 6 cut(s) 378, 956, 1109, 1751, 2021, 2198
Eco91I GGTNACC 4 cut(s) 1675, 1943, 2219, 2387
EcoICRI GAGCTC 1 cut(s) 1605
EcoO109I RGGNCCY 5 cut(s) 810, 1821, 2004, 2091, 2280
EcoO65I GGTNACC 4 cut(s) 1675, 1943, 2219, 2387
EcoRII CCWGG 5 cut(s) 159, 881, 1666, 1934, 2128
EcoT14I CCWWGG 6 cut(s) 40, 805, 1789, 1891, 2059, 2167
EcoT22I ATGCAT 5 cut(s) 138, 262, 1272, 1279, 2493
EcoT38I GRGCYC 5 cut(s) 1607, 1824, 2008, 2094, 2284
EgeI GGCGCC 2 cut(s) 616, 628
EheI GGCGCC 2 cut(s) 616, 628
ErhI CCWWGG 6 cut(s) 40, 805, 1789, 1891, 2059, 2167
FaqI GGGAC 5 cut(s) 823, 1126, 1730, 2000, 2448
FauI CCCGC 2 cut(s) 91, 1755
FbaI TGATCA 1 cut(s) 2494
FblI GTMKAC 2 cut(s) 211, 2076
Fnu4HI GCNGC 2 cut(s) 491, 2065
FokI GGATG 7 cut(s) 1131, 1683, 1838, 1951, 2102, 2227, 2405
FriOI GRGCYC 5 cut(s) 1607, 1824, 2008, 2094, 2284
Fsp4HI GCNGC 2 cut(s) 491, 2065
FspBI CTAG 3 cut(s) 1584, 2211, 2276
GlaI GCGC 6 cut(s) 445, 455, 479, 489, 616, 628
GluI GCNGC 2 cut(s) 491, 2065
GsaI CCCAGC 1 cut(s) 2003
HaeII RGCGCY 2 cut(s) 618, 630
HapII CCGG 7 cut(s) 113, 612, 618, 630, 1110, 1757, 2022
HgaI GACGC 3 cut(s) 165, 534, 2146
HhaI GCGC 6 cut(s) 446, 456, 480, 490, 617, 629
Hin1I GRCGYC 7 cut(s) 526, 615, 627, 770, 1815, 2085, 2157
Hin6I GCGC 6 cut(s) 444, 454, 478, 488, 615, 627
HinP1I GCGC 6 cut(s) 444, 454, 478, 488, 615, 627
HincII GTYRAC 3 cut(s) 520, 1182, 2077
HindII GTYRAC 3 cut(s) 520, 1182, 2077
HinfI GANTC 5 cut(s) 80, 580, 901, 1726, 2312
HpaI GTTAAC 1 cut(s) 1182
HpaII CCGG 7 cut(s) 113, 612, 618, 630, 1110, 1757, 2022
HphI GGTGA 8 cut(s) 73, 772, 1687, 1955, 2231, 2381, 2421, 2497
Hpy188I TCNGA 9 cut(s) 401, 798, 843, 1635, 1780, 1903, 2050, 2179, 2504
Hpy188III TCNNGA 8 cut(s) 533, 766, 916, 1242, 1409, 1685, 1953, 2229
Hpy99I CGWCG 8 cut(s) 626, 772, 853, 861, 1811, 1820, 2090, 2162
HpyAV CCTTC 5 cut(s) 70, 351, 675, 932, 2477
HpyCH4III ACNGT 6 cut(s) 515, 1179, 1722, 2264, 2308, 2532
HpyF10VI GCNNNNNNNGC 4 cut(s) 55, 161, 182, 2409
HpyF3I CTNAG 8 cut(s) 407, 908, 1067, 1461, 1510, 1632, 1826, 2096
Hsp92I GRCGYC 7 cut(s) 526, 615, 627, 770, 1815, 2085, 2157
HspAI GCGC 6 cut(s) 444, 454, 478, 488, 615, 627
KasI GGCGCC 2 cut(s) 614, 626
KroI GCCGGC 1 cut(s) 617
KroNI GCCGGC 1 cut(s) 619
Ksp22I TGATCA 1 cut(s) 2494
KspAI GTTAAC 1 cut(s) 1182
Kzo9I GATC 9 cut(s) 36, 714, 732, 762, 918, 1870, 2134, 2138, 2494
LmnI GCTCC 3 cut(s) 101, 807, 1602
LweI GCATC 4 cut(s) 225, 247, 1264, 2478
MaeI CTAG 3 cut(s) 1584, 2211, 2276
MalI GATC 9 cut(s) 38, 716, 734, 764, 920, 1872, 2136, 2140, 2496
MbiI CCGCTC 1 cut(s) 1750
MboI GATC 9 cut(s) 36, 714, 732, 762, 918, 1870, 2134, 2138, 2494
MboII GAAGA 6 cut(s) 945, 956, 959, 977, 1159, 2383
MfeI CAATTG 1 cut(s) 1466
MflI RGATCY 2 cut(s) 36, 1870
MhlI GDGCHC 7 cut(s) 106, 502, 1607, 1824, 2008, 2094, 2284
Mly113I GGCGCC 2 cut(s) 615, 627
MlyI GAGTC 4 cut(s) 74, 895, 1735, 2321
MmeI TCCRAC 1 cut(s) 2429
Mph1103I ATGCAT 5 cut(s) 138, 262, 1272, 1279, 2493
MreI CGCCGGCG 1 cut(s) 617
MroNI GCCGGC 1 cut(s) 617
MslI CAYNNNNRTG 4 cut(s) 71, 1444, 1992, 2496
MspI CCGG 7 cut(s) 113, 612, 618, 630, 1110, 1757, 2022
MunI CAATTG 1 cut(s) 1466
Mva1269I GAATGC 1 cut(s) 138
MvaI CCWGG 5 cut(s) 161, 883, 1668, 1936, 2130
MvnI CGCG 7 cut(s) 444, 454, 478, 488, 490, 1655, 2067
MwoI GCNNNNNNNGC 4 cut(s) 55, 161, 182, 2409
NaeI GCCGGC 1 cut(s) 619
NarI GGCGCC 2 cut(s) 615, 627
NciI CCSGG 6 cut(s) 114, 1110, 1111, 1758, 2022, 2023
NcoI CCATGG 2 cut(s) 1891, 2167
NdeII GATC 9 cut(s) 36, 714, 732, 762, 918, 1870, 2134, 2138, 2494
NgoMIV GCCGGC 1 cut(s) 617
NmeAIII GCCGAG 1 cut(s) 2052
NsiI ATGCAT 5 cut(s) 138, 262, 1272, 1279, 2493
NspI RCATGY 2 cut(s) 230, 1923
PaeI GCATGC 1 cut(s) 1923
PaeR7I CTCGAG 1 cut(s) 378
PasI CCCWGGG 1 cut(s) 2129
PceI AGGCCT 2 cut(s) 1594, 1862
PcsI WCGNNNNNNNCGW 3 cut(s) 767, 1803, 2082
PctI GAATGC 1 cut(s) 138
PdiI GCCGGC 1 cut(s) 619
PfeI GAWTC 1 cut(s) 580
Pfl23II CGTACG 1 cut(s) 1093
PflFI GACNNNGTC 3 cut(s) 515, 528, 847
PflMI CCANNNNNTGG 1 cut(s) 430
PfoI TCCNGGA 2 cut(s) 1666, 1934
PkrI GCNGC 2 cut(s) 492, 2066
PleI GAGTC 4 cut(s) 74, 895, 1734, 2320
PluTI GGCGCC 2 cut(s) 618, 630
PmaCI CACGTG 2 cut(s) 73, 497
PmlI CACGTG 2 cut(s) 73, 497
PpsI GAGTC 4 cut(s) 74, 895, 1734, 2320
Ppu21I YACGTR 3 cut(s) 73, 497, 1093
PpuMI RGGWCCY 1 cut(s) 810
PsiI TTATAA 1 cut(s) 1299
Psp124BI GAGCTC 1 cut(s) 1607
Psp5II RGGWCCY 1 cut(s) 810
Psp6I CCWGG 5 cut(s) 159, 881, 1666, 1934, 2128
PspCI CACGTG 2 cut(s) 73, 497
PspEI GGTNACC 4 cut(s) 1675, 1943, 2219, 2387
PspFI CCCAGC 1 cut(s) 1999
PspGI CCWGG 5 cut(s) 159, 881, 1666, 1934, 2128
PspLI CGTACG 1 cut(s) 1093
PspOMI GGGCCC 4 cut(s) 1820, 2004, 2090, 2280
PspPPI RGGWCCY 1 cut(s) 810
PspXI VCTCGAGB 1 cut(s) 378
PsuI RGATCY 2 cut(s) 36, 1870
PsyI GACNNNGTC 3 cut(s) 515, 528, 847
RsaI GTAC 2 cut(s) 388, 1095
RsaNI GTAC 2 cut(s) 387, 1094
RseI CAYNNNNRTG 4 cut(s) 71, 1444, 1992, 2496
SacI GAGCTC 1 cut(s) 1607
SalI GTCGAC 1 cut(s) 2075
SatI GCNGC 2 cut(s) 491, 2065
Sau3AI GATC 9 cut(s) 36, 714, 732, 762, 918, 1870, 2134, 2138, 2494
SchI GAGTC 4 cut(s) 74, 895, 1735, 2321
SduI GDGCHC 7 cut(s) 106, 502, 1607, 1824, 2008, 2094, 2284
SfaNI GCATC 4 cut(s) 225, 247, 1264, 2478
SfoI GGCGCC 2 cut(s) 616, 628
Sfr274I CTCGAG 1 cut(s) 378
SgrAI CRCCGGYG 1 cut(s) 617
SinI GGWCC 8 cut(s) 433, 470, 810, 843, 1016, 1744, 2014, 2462
SlaI CTCGAG 1 cut(s) 378
SmaI CCCGGG 2 cut(s) 1111, 2023
SmiMI CAYNNNNRTG 4 cut(s) 71, 1444, 1992, 2496
SmlI CTYRAG 1 cut(s) 378
SmoI CTYRAG 1 cut(s) 378
SphI GCATGC 1 cut(s) 1923
SseBI AGGCCT 2 cut(s) 1594, 1862
SsiI CCGC 7 cut(s) 98, 153, 490, 1703, 1748, 2065, 2396
SspDI GGCGCC 2 cut(s) 614, 626
SspI AATATT 4 cut(s) 539, 560, 603, 2297
SspMI CTAG 3 cut(s) 1584, 2211, 2276
SstI GAGCTC 1 cut(s) 1607
StuI AGGCCT 2 cut(s) 1594, 1862
StyI CCWWGG 6 cut(s) 40, 805, 1789, 1891, 2059, 2167
TaaI ACNGT 6 cut(s) 515, 1179, 1722, 2264, 2308, 2532
TaqI TCGA 4 cut(s) 379, 851, 2076, 2137
TaqII GACCGA 1 cut(s) 2305
TauI GCSGC 2 cut(s) 493, 2067
TfiI GAWTC 1 cut(s) 580
TscAI CASTG 9 cut(s) 351, 507, 514, 594, 902, 1182, 1614, 1882, 2158
TspDTI ATGAA 7 cut(s) 161, 240, 720, 966, 1160, 1612, 2407
TspGWI ACGGA 7 cut(s) 340, 483, 553, 569, 573, 911, 1166
TspMI CCCGGG 2 cut(s) 1109, 2021
TspRI CASTG 9 cut(s) 351, 507, 514, 594, 902, 1182, 1614, 1882, 2158
Tth111I GACNNNGTC 3 cut(s) 515, 528, 847
Van91I CCANNNNNTGG 1 cut(s) 430
VneI GTGCAC 1 cut(s) 498
VpaK11BI GGWCC 8 cut(s) 433, 470, 810, 843, 1016, 1744, 2014, 2462
XapI RAATTY 5 cut(s) 301, 642, 681, 1084, 1535
XceI RCATGY 2 cut(s) 230, 1923
XcmI CCANNNNNNNNNTGG 1 cut(s) 2439
XhoI CTCGAG 1 cut(s) 378
XmaI CCCGGG 2 cut(s) 1109, 2021
XmiI GTMKAC 2 cut(s) 211, 2076
XspI CTAG 3 cut(s) 1584, 2211, 2276
ZraI GACGTC 3 cut(s) 771, 1816, 2086
Zsp2I ATGCAT 5 cut(s) 138, 262, 1272, 1279, 2493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.