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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 2106 |
| Acc16I | TGCGCA | 1 cut(s) | 1969 |
| Acc36I | ACCTGC | 2 cut(s) | 737, 893 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 612 |
| AccII | CGCG | 2 cut(s) | 566, 801 |
| AccIII | TCCGGA | 1 cut(s) | 1210 |
| AciI | CCGC | 5 cut(s) | 566, 766, 799, 1550, 2004 |
| AclWI | GGATC | 5 cut(s) | 496, 781, 794, 1460, 1731 |
| AcsI | RAATTY | 6 cut(s) | 269, 318, 1715, 1906, 2056, 2115 |
| AcuI | CTGAAG | 1 cut(s) | 1792 |
| AcvI | CACGTG | 2 cut(s) | 751, 823 |
| AfaI | GTAC | 6 cut(s) | 244, 844, 870, 948, 1311, 2072 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 612, 1182, 1341, 1496, 1497, 1498, 1526, 1527, 1653 |
| AflIII | ACRYGT | 2 cut(s) | 1266, 1856 |
| AgsI | TTSAA | 5 cut(s) | 184, 281, 1048, 1394, 1994 |
| AjiI | CACGTC | 1 cut(s) | 1859 |
| AjnI | CCWGG | 3 cut(s) | 1520, 1732, 2082 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1937, 1969 |
| AluBI | AGCT | 6 cut(s) | 132, 721, 932, 1292, 1776, 1965 |
| AluI | AGCT | 6 cut(s) | 132, 721, 932, 1292, 1776, 1965 |
| Alw21I | GWGCWC | 2 cut(s) | 723, 1235 |
| Alw26I | GTCTC | 2 cut(s) | 538, 2084 |
| AlwI | GGATC | 5 cut(s) | 496, 781, 794, 1460, 1731 |
| Ama87I | CYCGRG | 3 cut(s) | 553, 573, 1543 |
| Aor13HI | TCCGGA | 1 cut(s) | 1210 |
| AoxI | GGCC | 4 cut(s) | 1414, 1500, 1517, 1524 |
| ApaI | GGGCCC | 1 cut(s) | 1504 |
| ApeKI | GCWGC | 1 cut(s) | 1965 |
| ApoI | RAATTY | 6 cut(s) | 269, 318, 1715, 1906, 2056, 2115 |
| AspLEI | GCGC | 3 cut(s) | 566, 1088, 1970 |
| AspS9I | GGNCC | 5 cut(s) | 1173, 1500, 1501, 1518, 1524 |
| AsuC2I | CCSGG | 3 cut(s) | 490, 1142, 1528 |
| AsuHPI | GGTGA | 4 cut(s) | 553, 751, 1154, 1636 |
| AsuII | TTCGAA | 2 cut(s) | 1374, 1686 |
| AvaI | CYCGRG | 3 cut(s) | 553, 573, 1543 |
| AvaII | GGWCC | 1 cut(s) | 1173 |
| BaeGI | GKGCMC | 1 cut(s) | 1504 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 2054, 2087 |
| BamHI | GGATCC | 1 cut(s) | 786 |
| BanII | GRGCYC | 3 cut(s) | 723, 1504, 1550 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1191, 1223 |
| BauI | CACGAG | 3 cut(s) | 895, 1418, 1623 |
| BbrPI | CACGTG | 2 cut(s) | 751, 823 |
| BbsI | GAAGAC | 2 cut(s) | 343, 1218 |
| Bbv12I | GWGCWC | 2 cut(s) | 723, 1235 |
| BbvI | GCAGC | 1 cut(s) | 1952 |
| BccI | CCATC | 3 cut(s) | 913, 1344, 1656 |
| BceAI | ACGGC | 1 cut(s) | 929 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 881, 915 |
| BciT130I | CCWGG | 3 cut(s) | 1522, 1734, 2084 |
| BcnI | CCSGG | 3 cut(s) | 490, 1142, 1528 |
| BcoDI | GTCTC | 2 cut(s) | 538, 2084 |
| BfmI | CTRYAG | 2 cut(s) | 1561, 2091 |
| BfuAI | ACCTGC | 2 cut(s) | 737, 893 |
| BisI | GCNGC | 1 cut(s) | 1966 |
| BlsI | GCNGC | 1 cut(s) | 1967 |
| Bme1390I | CCNGG | 6 cut(s) | 490, 1142, 1522, 1528, 1734, 2084 |
| Bme18I | GGWCC | 1 cut(s) | 1173 |
| BmeT110I | CYCGRG | 3 cut(s) | 553, 573, 1543 |
| BmgBI | CACGTC | 1 cut(s) | 1859 |
| BmgT120I | GGNCC | 5 cut(s) | 1173, 1500, 1501, 1518, 1524 |
| BmiI | GGNNCC | 6 cut(s) | 788, 889, 1125, 1501, 1502, 1701 |
| BmrFI | CCNGG | 6 cut(s) | 490, 1142, 1522, 1528, 1734, 2084 |
| BmrI | ACTGGG | 2 cut(s) | 852, 1630 |
| BmsI | GCATC | 6 cut(s) | 173, 204, 482, 699, 2041, 2188 |
| BmuI | ACTGGG | 2 cut(s) | 852, 1630 |
| BpiI | GAAGAC | 2 cut(s) | 343, 1218 |
| BpmI | CTGGAG | 1 cut(s) | 2066 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1777 |
| Bpu14I | TTCGAA | 2 cut(s) | 1374, 1686 |
| BpuEI | CTTGAG | 2 cut(s) | 401, 857 |
| BpuMI | CCSGG | 3 cut(s) | 490, 1142, 1528 |
| BsaAI | YACGTR | 3 cut(s) | 67, 751, 823 |
| BsaBI | GATNNNNATC | 2 cut(s) | 150, 472 |
| BsaI | GGTCTC | 1 cut(s) | 538 |
| BsaJI | CCNNGG | 9 cut(s) | 488, 554, 736, 1494, 1520, 1521, 1527, 1732, 1733 |
| BsaWI | WCCGGW | 1 cut(s) | 1210 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 612, 1182, 1341, 1496, 1497, 1498, 1526, 1527, 1653 |
| Bse118I | RCCGGY | 1 cut(s) | 2028 |
| Bse1I | ACTGG | 7 cut(s) | 39, 606, 858, 1043, 1398, 1636, 1710 |
| Bse8I | GATNNNNATC | 2 cut(s) | 150, 472 |
| BseAI | TCCGGA | 1 cut(s) | 1210 |
| BseBI | CCWGG | 3 cut(s) | 1522, 1734, 2084 |
| BseDI | CCNNGG | 9 cut(s) | 488, 554, 736, 1494, 1520, 1521, 1527, 1732, 1733 |
| BseGI | GGATG | 4 cut(s) | 473, 1438, 1481, 1752 |
| BseJI | GATNNNNATC | 2 cut(s) | 150, 472 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 612, 1182, 1341, 1496, 1497, 1498, 1526, 1527, 1653 |
| BseMII | CTCAG | 1 cut(s) | 984 |
| BseNI | ACTGG | 7 cut(s) | 39, 606, 858, 1043, 1398, 1636, 1710 |
| BseSI | GKGCMC | 1 cut(s) | 1504 |
| BseXI | GCAGC | 1 cut(s) | 1952 |
| BseYI | CCCAGC | 1 cut(s) | 812 |
| BsgI | GTGCAG | 2 cut(s) | 715, 921 |
| Bsh1236I | CGCG | 2 cut(s) | 566, 801 |
| BshFI | GGCC | 4 cut(s) | 1416, 1502, 1519, 1526 |
| BsiHKAI | GWGCWC | 2 cut(s) | 723, 1235 |
| BsiHKCI | CYCGRG | 3 cut(s) | 553, 573, 1543 |
| BsiSI | CCGG | 5 cut(s) | 490, 1142, 1211, 1527, 2029 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 612, 1182, 1341, 1496, 1497, 1498, 1526, 1527, 1653 |
| BsmAI | GTCTC | 2 cut(s) | 538, 2084 |
| BsnI | GGCC | 4 cut(s) | 1416, 1502, 1519, 1526 |
| Bso31I | GGTCTC | 1 cut(s) | 538 |
| BsoBI | CYCGRG | 3 cut(s) | 553, 573, 1543 |
| Bsp119I | TTCGAA | 2 cut(s) | 1374, 1686 |
| Bsp120I | GGGCCC | 1 cut(s) | 1500 |
| Bsp1286I | GDGCHC | 4 cut(s) | 723, 1235, 1504, 1550 |
| Bsp13I | TCCGGA | 1 cut(s) | 1210 |
| Bsp143I | GATC | 6 cut(s) | 501, 786, 825, 1402, 1452, 1723 |
| BspACI | CCGC | 5 cut(s) | 566, 766, 799, 1550, 2004 |
| BspANI | GGCC | 4 cut(s) | 1416, 1502, 1519, 1526 |
| BspCNI | CTCAG | 1 cut(s) | 985 |
| BspEI | TCCGGA | 1 cut(s) | 1210 |
| BspFNI | CGCG | 2 cut(s) | 566, 801 |
| BspLI | GGNNCC | 6 cut(s) | 788, 889, 1125, 1501, 1502, 1701 |
| BspMI | ACCTGC | 2 cut(s) | 737, 893 |
| BspPI | GGATC | 5 cut(s) | 496, 781, 794, 1460, 1731 |
| BspT104I | TTCGAA | 2 cut(s) | 1374, 1686 |
| BspTNI | GGTCTC | 1 cut(s) | 538 |
| BsrFI | RCCGGY | 1 cut(s) | 2028 |
| BsrI | ACTGG | 7 cut(s) | 39, 606, 858, 1043, 1398, 1636, 1710 |
| BssAI | RCCGGY | 1 cut(s) | 2028 |
| BssECI | CCNNGG | 9 cut(s) | 488, 554, 736, 1494, 1520, 1521, 1527, 1732, 1733 |
| BssMI | GATC | 6 cut(s) | 501, 786, 825, 1402, 1452, 1723 |
| BssSI | CACGAG | 3 cut(s) | 895, 1418, 1623 |
| BssT1I | CCWWGG | 1 cut(s) | 1494 |
| Bst2BI | CACGAG | 3 cut(s) | 895, 1418, 1623 |
| Bst2UI | CCWGG | 3 cut(s) | 1522, 1734, 2084 |
| Bst4CI | ACNGT | 2 cut(s) | 1562, 1604 |
| BstBAI | YACGTR | 3 cut(s) | 67, 751, 823 |
| BstBI | TTCGAA | 2 cut(s) | 1374, 1686 |
| BstC8I | GCNNGC | 5 cut(s) | 1110, 1262, 1550, 1916, 2155 |
| BstDEI | CTNAG | 5 cut(s) | 21, 296, 993, 1463, 1777 |
| BstEII | GGTNACC | 1 cut(s) | 1642 |
| BstF5I | GGATG | 4 cut(s) | 473, 1438, 1481, 1752 |
| BstFNI | CGCG | 2 cut(s) | 566, 801 |
| BstHHI | GCGC | 3 cut(s) | 566, 1088, 1970 |
| BstKTI | GATC | 6 cut(s) | 504, 789, 828, 1405, 1455, 1726 |
| BstMAI | GTCTC | 2 cut(s) | 538, 2084 |
| BstMBI | GATC | 6 cut(s) | 501, 786, 825, 1402, 1452, 1723 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 690, 718 |
| BstNI | CCWGG | 3 cut(s) | 1522, 1734, 2084 |
| BstNSI | RCATGY | 2 cut(s) | 1270, 2157 |
| BstPI | GGTNACC | 1 cut(s) | 1642 |
| BstSCI | CCNGG | 6 cut(s) | 488, 1140, 1520, 1526, 1732, 2082 |
| BstSFI | CTRYAG | 2 cut(s) | 1561, 2091 |
| BstSLI | GKGCMC | 1 cut(s) | 1504 |
| BstUI | CGCG | 2 cut(s) | 566, 801 |
| BstV1I | GCAGC | 1 cut(s) | 1952 |
| BstV2I | GAAGAC | 2 cut(s) | 343, 1218 |
| BstX2I | RGATCY | 1 cut(s) | 786 |
| BstYI | RGATCY | 1 cut(s) | 786 |
| BsuRI | GGCC | 4 cut(s) | 1416, 1502, 1519, 1526 |
| BtgZI | GCGATG | 2 cut(s) | 227, 1753 |
| BtrI | CACGTC | 1 cut(s) | 1859 |
| BtsCI | GGATG | 4 cut(s) | 473, 1438, 1481, 1752 |
| BtsIMutI | CAGTG | 5 cut(s) | 613, 1405, 1558, 1643, 1717 |
| BveI | ACCTGC | 2 cut(s) | 737, 893 |
| Cac8I | GCNNGC | 5 cut(s) | 1110, 1262, 1550, 1916, 2155 |
| CfoI | GCGC | 3 cut(s) | 566, 1088, 1970 |
| Cfr10I | RCCGGY | 1 cut(s) | 2028 |
| Cfr13I | GGNCC | 5 cut(s) | 1173, 1500, 1501, 1518, 1524 |
| CseI | GACGC | 1 cut(s) | 699 |
| Csp6I | GTAC | 6 cut(s) | 243, 843, 869, 947, 1310, 2071 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1476, 1511 |
| CviAII | CATG | 8 cut(s) | 339, 981, 998, 1267, 1340, 1652, 2016, 2154 |
| CviQI | GTAC | 6 cut(s) | 243, 843, 869, 947, 1310, 2071 |
| DdeI | CTNAG | 5 cut(s) | 21, 296, 993, 1463, 1777 |
| DpnI | GATC | 6 cut(s) | 503, 788, 827, 1404, 1454, 1725 |
| DpnII | GATC | 6 cut(s) | 501, 786, 825, 1402, 1452, 1723 |
| EciI | GGCGGA | 1 cut(s) | 2019 |
| Ecl136II | GAGCTC | 1 cut(s) | 721 |
| Eco130I | CCWWGG | 1 cut(s) | 1494 |
| Eco147I | AGGCCT | 1 cut(s) | 1416 |
| Eco24I | GRGCYC | 3 cut(s) | 723, 1504, 1550 |
| Eco31I | GGTCTC | 1 cut(s) | 538 |
| Eco32I | GATATC | 1 cut(s) | 2013 |
| Eco47I | GGWCC | 1 cut(s) | 1173 |
| Eco53kI | GAGCTC | 1 cut(s) | 721 |
| Eco57I | CTGAAG | 1 cut(s) | 1792 |
| Eco72I | CACGTG | 2 cut(s) | 751, 823 |
| Eco88I | CYCGRG | 3 cut(s) | 553, 573, 1543 |
| Eco91I | GGTNACC | 1 cut(s) | 1642 |
| EcoICRI | GAGCTC | 1 cut(s) | 721 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1500 |
| EcoO65I | GGTNACC | 1 cut(s) | 1642 |
| EcoRI | GAATTC | 1 cut(s) | 2056 |
| EcoRII | CCWGG | 3 cut(s) | 1520, 1732, 2082 |
| EcoRV | GATATC | 1 cut(s) | 2013 |
| EcoT14I | CCWWGG | 1 cut(s) | 1494 |
| EcoT22I | ATGCAT | 3 cut(s) | 166, 714, 2159 |
| EcoT38I | GRGCYC | 3 cut(s) | 723, 1504, 1550 |
| ErhI | CCWWGG | 1 cut(s) | 1494 |
| FaeI | CATG | 8 cut(s) | 342, 984, 1001, 1270, 1343, 1655, 2019, 2157 |
| FatI | CATG | 8 cut(s) | 338, 980, 997, 1266, 1339, 1651, 2015, 2153 |
| FauI | CCCGC | 3 cut(s) | 773, 806, 1557 |
| Fnu4HI | GCNGC | 1 cut(s) | 1966 |
| FokI | GGATG | 4 cut(s) | 460, 1445, 1488, 1759 |
| FriOI | GRGCYC | 3 cut(s) | 723, 1504, 1550 |
| Fsp4HI | GCNGC | 1 cut(s) | 1966 |
| FspI | TGCGCA | 1 cut(s) | 1969 |
| GlaI | GCGC | 3 cut(s) | 565, 1087, 1969 |
| GluI | GCNGC | 1 cut(s) | 1966 |
| GsaI | CCCAGC | 1 cut(s) | 816 |
| GsuI | CTGGAG | 1 cut(s) | 2066 |
| HaeIII | GGCC | 4 cut(s) | 1416, 1502, 1519, 1526 |
| HapII | CCGG | 5 cut(s) | 490, 1142, 1211, 1527, 2029 |
| HgaI | GACGC | 1 cut(s) | 699 |
| HhaI | GCGC | 3 cut(s) | 566, 1088, 1970 |
| Hin1II | CATG | 8 cut(s) | 342, 984, 1001, 1270, 1343, 1655, 2019, 2157 |
| Hin6I | GCGC | 3 cut(s) | 564, 1086, 1968 |
| HinP1I | GCGC | 3 cut(s) | 564, 1086, 1968 |
| HindIII | AAGCTT | 2 cut(s) | 130, 1774 |
| HinfI | GANTC | 9 cut(s) | 311, 362, 383, 392, 431, 452, 893, 938, 1835 |
| HpaII | CCGG | 5 cut(s) | 490, 1142, 1211, 1527, 2029 |
| HphI | GGTGA | 4 cut(s) | 553, 751, 1154, 1636 |
| Hpy166II | GTNNAC | 3 cut(s) | 689, 759, 2203 |
| Hpy188I | TCNGA | 6 cut(s) | 892, 994, 1152, 1237, 1811, 1977 |
| Hpy188III | TCNNGA | 8 cut(s) | 495, 553, 935, 1211, 1543, 1623, 1901, 2121 |
| Hpy8I | GTNNAC | 3 cut(s) | 689, 759, 2203 |
| HpyAV | CCTTC | 7 cut(s) | 605, 755, 827, 1369, 1681, 1816, 1849 |
| HpyCH4III | ACNGT | 2 cut(s) | 1562, 1604 |
| HpyCH4IV | ACGT | 6 cut(s) | 66, 750, 822, 1507, 1858, 2069 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 690, 718 |
| HpyF3I | CTNAG | 5 cut(s) | 21, 296, 993, 1463, 1777 |
| HpySE526I | ACGT | 6 cut(s) | 66, 750, 822, 1507, 1858, 2069 |
| Hsp92II | CATG | 8 cut(s) | 342, 984, 1001, 1270, 1343, 1655, 2019, 2157 |
| HspAI | GCGC | 3 cut(s) | 564, 1086, 1968 |
| Kpn2I | TCCGGA | 1 cut(s) | 1210 |
| Kzo9I | GATC | 6 cut(s) | 501, 786, 825, 1402, 1452, 1723 |
| LmnI | GCTCC | 2 cut(s) | 1129, 1733 |
| Lsp1109I | GCAGC | 1 cut(s) | 1952 |
| LweI | GCATC | 6 cut(s) | 173, 204, 482, 699, 2041, 2188 |
| MaeII | ACGT | 6 cut(s) | 66, 750, 822, 1507, 1858, 2069 |
| MaeIII | GTNAC | 9 cut(s) | 110, 529, 568, 586, 793, 1478, 1642, 1749, 2142 |
| MalI | GATC | 6 cut(s) | 503, 788, 827, 1404, 1454, 1725 |
| MboI | GATC | 6 cut(s) | 501, 786, 825, 1402, 1452, 1723 |
| MboII | GAAGA | 7 cut(s) | 343, 1045, 1199, 1218, 1889, 1941, 2158 |
| MfeI | CAATTG | 1 cut(s) | 525 |
| MflI | RGATCY | 1 cut(s) | 786 |
| MhlI | GDGCHC | 4 cut(s) | 723, 1235, 1504, 1550 |
| MlyI | GAGTC | 7 cut(s) | 320, 371, 392, 401, 440, 461, 887 |
| MmeI | TCCRAC | 1 cut(s) | 915 |
| Mph1103I | ATGCAT | 3 cut(s) | 166, 714, 2159 |
| MroI | TCCGGA | 1 cut(s) | 1210 |
| MslI | CAYNNNNRTG | 3 cut(s) | 606, 1269, 2184 |
| MspA1I | CMGCKG | 1 cut(s) | 1965 |
| MspI | CCGG | 5 cut(s) | 490, 1142, 1211, 1527, 2029 |
| MspR9I | CCNGG | 6 cut(s) | 490, 1142, 1522, 1528, 1734, 2084 |
| MunI | CAATTG | 1 cut(s) | 525 |
| MvaI | CCWGG | 3 cut(s) | 1522, 1734, 2084 |
| MvnI | CGCG | 2 cut(s) | 566, 801 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 690, 718 |
| NciI | CCSGG | 3 cut(s) | 490, 1142, 1528 |
| NdeII | GATC | 6 cut(s) | 501, 786, 825, 1402, 1452, 1723 |
| NlaIII | CATG | 8 cut(s) | 342, 984, 1001, 1270, 1343, 1655, 2019, 2157 |
| NlaIV | GGNNCC | 6 cut(s) | 788, 889, 1125, 1501, 1502, 1701 |
| NmuCI | GTSAC | 6 cut(s) | 110, 529, 568, 793, 1642, 2142 |
| NsbI | TGCGCA | 1 cut(s) | 1969 |
| NsiI | ATGCAT | 3 cut(s) | 166, 714, 2159 |
| NspI | RCATGY | 2 cut(s) | 1270, 2157 |
| NspV | TTCGAA | 2 cut(s) | 1374, 1686 |
| PaeI | GCATGC | 1 cut(s) | 2157 |
| PaeR7I | CTCGAG | 1 cut(s) | 573 |
| PasI | CCCWGGG | 2 cut(s) | 1521, 1733 |
| PceI | AGGCCT | 1 cut(s) | 1416 |
| PciI | ACATGT | 1 cut(s) | 1266 |
| PfeI | GAWTC | 2 cut(s) | 938, 1835 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 612 |
| PkrI | GCNGC | 1 cut(s) | 1967 |
| PleI | GAGTC | 7 cut(s) | 319, 370, 391, 400, 439, 460, 887 |
| PmaCI | CACGTG | 2 cut(s) | 751, 823 |
| PmlI | CACGTG | 2 cut(s) | 751, 823 |
| PpsI | GAGTC | 7 cut(s) | 319, 370, 391, 400, 439, 460, 887 |
| Ppu21I | YACGTR | 3 cut(s) | 67, 751, 823 |
| PscI | ACATGT | 1 cut(s) | 1266 |
| PsiI | TTATAA | 1 cut(s) | 2106 |
| Psp124BI | GAGCTC | 1 cut(s) | 723 |
| Psp6I | CCWGG | 3 cut(s) | 1520, 1732, 2082 |
| PspCI | CACGTG | 2 cut(s) | 751, 823 |
| PspEI | GGTNACC | 1 cut(s) | 1642 |
| PspFI | CCCAGC | 1 cut(s) | 812 |
| PspGI | CCWGG | 3 cut(s) | 1520, 1732, 2082 |
| PspN4I | GGNNCC | 6 cut(s) | 788, 889, 1125, 1501, 1502, 1701 |
| PspOMI | GGGCCC | 1 cut(s) | 1500 |
| PspPI | GGNCC | 5 cut(s) | 1173, 1500, 1501, 1518, 1524 |
| PspXI | VCTCGAGB | 1 cut(s) | 573 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 490, 522 |
| PsuI | RGATCY | 1 cut(s) | 786 |
| PvuII | CAGCTG | 1 cut(s) | 1965 |
| RsaI | GTAC | 6 cut(s) | 244, 844, 870, 948, 1311, 2072 |
| RsaNI | GTAC | 6 cut(s) | 243, 843, 869, 947, 1310, 2071 |
| RseI | CAYNNNNRTG | 3 cut(s) | 606, 1269, 2184 |
| SacI | GAGCTC | 1 cut(s) | 723 |
| SatI | GCNGC | 1 cut(s) | 1966 |
| Sau3AI | GATC | 6 cut(s) | 501, 786, 825, 1402, 1452, 1723 |
| Sau96I | GGNCC | 5 cut(s) | 1173, 1500, 1501, 1518, 1524 |
| SchI | GAGTC | 7 cut(s) | 320, 371, 392, 401, 440, 461, 887 |
| ScrFI | CCNGG | 6 cut(s) | 490, 1142, 1522, 1528, 1734, 2084 |
| SduI | GDGCHC | 4 cut(s) | 723, 1235, 1504, 1550 |
| SfaNI | GCATC | 6 cut(s) | 173, 204, 482, 699, 2041, 2188 |
| SfcI | CTRYAG | 2 cut(s) | 1561, 2091 |
| Sfr274I | CTCGAG | 1 cut(s) | 573 |
| SfuI | TTCGAA | 2 cut(s) | 1374, 1686 |
| SinI | GGWCC | 1 cut(s) | 1173 |
| SlaI | CTCGAG | 1 cut(s) | 573 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 606, 1269, 2184 |
| SmlI | CTYRAG | 3 cut(s) | 380, 573, 836 |
| SmoI | CTYRAG | 3 cut(s) | 380, 573, 836 |
| SphI | GCATGC | 1 cut(s) | 2157 |
| SseBI | AGGCCT | 1 cut(s) | 1416 |
| SsiI | CCGC | 5 cut(s) | 566, 766, 799, 1550, 2004 |
| SstI | GAGCTC | 1 cut(s) | 723 |
| StuI | AGGCCT | 1 cut(s) | 1416 |
| StyD4I | CCNGG | 6 cut(s) | 488, 1140, 1520, 1526, 1732, 2082 |
| StyI | CCWWGG | 1 cut(s) | 1494 |
| TaaI | ACNGT | 2 cut(s) | 1562, 1604 |
| TaiI | ACGT | 6 cut(s) | 69, 753, 825, 1510, 1861, 2072 |
| TaqI | TCGA | 6 cut(s) | 574, 968, 1374, 1382, 1686, 1889 |
| TfiI | GAWTC | 2 cut(s) | 938, 1835 |
| TscAI | CASTG | 5 cut(s) | 613, 1405, 1565, 1643, 1717 |
| TseFI | GTSAC | 6 cut(s) | 110, 529, 568, 793, 1642, 2142 |
| TseI | GCWGC | 1 cut(s) | 1965 |
| Tsp45I | GTSAC | 6 cut(s) | 110, 529, 568, 793, 1642, 2142 |
| TspDTI | ATGAA | 4 cut(s) | 17, 343, 1400, 1941 |
| TspGWI | ACGGA | 2 cut(s) | 618, 1712 |
| TspRI | CASTG | 5 cut(s) | 613, 1405, 1565, 1643, 1717 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 612 |
| VpaK11BI | GGWCC | 1 cut(s) | 1173 |
| XapI | RAATTY | 6 cut(s) | 269, 318, 1715, 1906, 2056, 2115 |
| XbaI | TCTAGA | 2 cut(s) | 494, 934 |
| XceI | RCATGY | 2 cut(s) | 1270, 2157 |
| XhoI | CTCGAG | 1 cut(s) | 573 |
| Zsp2I | ATGCAT | 3 cut(s) | 166, 714, 2159 |