pycom17g16100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0000158
Physical Location & Seq
Forward (+)
14367758 .. 14372584
4827 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g16100.8

Sequence Viewer

Length: 3474 bp
ATGCTAGCTGCTAGTCGGGCGGCAGGTTGGGGCCTAGCGGATTTAAGTAAATCTGTTATATTTCATATAAATAAGTGTCACATCTCGGCCCGGGATTGCTCTAGCCCCTTCTCACTTAACTTCGGAGTTCCTACGGAACCCGAAACCAGTGAGCTCCCAAAAGGCCTCGTGCTAGGTAGGGATGGGAATATACATTTAAGGATCACTCCCTTAGGCGATGTGGGATGTCACAATCCACCCCCCTTAGGGGCCCAACGTCCTCGTCGGCACACCACAACCAGGCATTTCCCGGGCCCGCTCCACCACCGTAGCACGATATTGTCCGCTTTGGGCTTACCATTCCCTCACGGTTTTGTTTTTGGGAACTCACGAGCAACTTCCTGGTGGGTCACCCATCATGGGATTGCTCTAGCCCCCTTCTCGCTTAACTTCAGAGTTCCTACGGAACCCGAAGCCAGTGAGCTCCCAAAAGGCCTCGTGCTAGGTAGGGATGGGAATATACATTTAAGGATCACTCCCTTGGGTGATGTGGGATGTCACAATAAGTGTCTATATTATGAAGTATTGAAACATAATGAAAACATAGGGAACAAGCATATAATGTGTGTTCATTTAAGTATTCAACAAGTCTCTTACAATTTATTAAAAAACATAATGCAAAATGAAAGTTATCTATTTTCTGCGGGCGCCTCAGCAGGTCTAGGTACCCTTTGTAAATTTTCAAACGCAGGGAGTTTTAAAGCAGTGATGGTAATATTGCACTCTCGGGAATTTATTACTTGCTTATCGAGACAACAATGCATTTTGTTGGATTCGTTAAACAAGTGGACAAATAGGTCATTTTACGTTAGCATTTTAGTTATTATTTATTGTTTGGGCTTGACCCAAGCATACTACACATTTATCTTTGGAGTATGTGATGCAATGAGTGTTATTATATATATTTATTTTGACGTGATGTTATTATACATATATAAATATTTTCGACTTTATAAGGAAAGTATGAGTTTGGGGTCTCTTTTATGCAACCACACTAACTTGATTTCTTCTTTATATATATTTCTTTCTATCTTTGAGTATTTTATGTATAGCAATGTGATAACCATTGACGAAAACTCCCTAGGGGTGAAACTGTTGACTTTCGTTGACCATCATTTTGTGACGTGTACAAGCTACTATTTTACCGTATTCTCGAAGTACTCGGCGATACAAATGTGTAAGCGCAAGGCGTGTACCATGTGGGGCCCACACCTCACTCCTCTCCCTCACCCCGTGTCTCCCCTTCTTTTCCCCATTTTCAGGAAACACTTTCTCTCTCTCTTCTCACTATCTCCTCCAACACCTCTCCTTTTCTTCTTTTCCAACTCTCTCCCTCTGCAAAAATTGGCTAACACACCTCGCCGCTTTGGCGAGCTTCACCATACCATCACCATAACTCTCTATCCATCTCTGTTAACTCTCTTTAACTCTCCCAGTTCTCCCTTTCCCTCACTTTCTCCTTCGGAGTCGTTCCTCACTCGGCAACATTTATCAGCCGTGAGGCCGAGATCACCACCACTAACCTGCACACCTTGTCCCTTTCCAAGATTTAAATCAGAAGATAACAAACGAAGCTCCGATATCGAACACGTGTTCTTGGTTTGTATTTCTAGCTTTTCTAAGATTTTTGGTGGGGGTTTTGGCACTGAAACGGCATGGGGGAAATTTCTCCATATTTTCCAGCGAACCCGGGAAAGTTGCAAGTGTTTTTCCAGCCTACTCCGACCACGACCGGAAGTTGTAATGGTATACTTCGATTCCTCATCTTTTAAGCTTGAATTTGGTATATTAGGTGAACTTCTGGCTTTCTCCTTCCTAGAAACCACTGGTTGGTTTCCAAAAAAAATAAAAAAAAATTTAACTGTGATCCGGTTCCAAACCTGGGATCCAAGGGAATATTCTGTAAACCTTCCAGTGAACTTTAACAGAATATTTCATCTAAGAATGGAGTATTCCGTTAAAGTTAACTTTCGACATCTCGAGTCCATTTTTGACATGTTGACCGTTGACTTTTTCATTGAAAATTTACTGAGGACCCTCCTTAGCGTATTTCGACGCCCTGATTCCAAATCCTTATTCTGTTTCCCCAAATTTAATAATCTTAGTGAAGTTTTACTAATGGGTCCTAATATTATGCTTTGGTGCGATTACTATCGAAGACTCTGGCCATCCTATTTCTTTGATATATTTATTTATGCTGTAATTAAATTTACATTTATAGGAAATGACGCCTCAATTAGAATTACGGTTATGGATAATATTATAGTGATTAGAATTACGGTTATGGATAATATTATATTGACTAGAATTATCGAATCACTATGGCATGACTATATTTATGTTATCTGCTCATCGTTTGCTCCACCAGTGTTAATACTCGCTCGAGCCAGGGCCAGTCTTTCACGTGTATGTGCACATCGCAGCATACGCTCACTTTGGATCTATTGTAGGTGCTATACTAGAGCGTATATCATTGTTACACGTAGTCATGTTCATGTCAGAATATCTCTACATGAACTGGTGTGTCAGCATCTGTCGATGAGCACTCGATATGATACGTTTATTTATGTGAGAATATATGATTATAGACTTTATGTATTTATTTTCTCTGGTAAGTATTTTCATACTATACATAGTATGTATATTTTGGGAACTATACTTATTTTACGGCGAGGGGTTACTATGTTTTCGAAAAGGTTTTTACGAATCTTTATTTTCAGGCCCACTCACCTTGTTTTTCATCCCTCAAGGATTGAGTGGCTGAGTGATTACCTTCAATGGTATAATTCTCATCCTACTTTACTTTACTTATGCTCTGTCATCACGTGTGAAATGGGTTCATTTCCGCTCATAAGCGCACTCTTATACACTACACTTTATGGCTTCGTCTTCCTCTGGGTGTCGACCAGCATAGTTAGATTCGGAGTCCAGGTAGACATTCCGGGTCAGGGTGTGTCAATGTACCAGTCGAATGCAATGCCTTTAAGTGAGTGGATGAATTGCTTCTGTTTAGGGTTGCCTTTTCCATTGAATTGCAAGAATTTGGGAGGTTGGTACGCAGTCAGCATTCGGAGATTATCAAGCTTCTTCGTGTACGATTTGGACATCTCAGGAAGAACCTCCATATTGTGCTTTGGTAGTGTGGCGATTATGTTTTGGAGTTGTTGGATTGACAAAGACACCACTAAAGCGAAATTAGTCTTTGATTGTGCACCTTTGCTCGACTCCTCGTCATGCCTTTCTTCTAACTTGCTCATAACCGACGCTATATGTATGTCTTTCTCCTCAATGGTCTTTGTCACTAAAGTAACTCCTACCATCATCACTTGCATGGAATCTAGGCATAGGCGCGCTTTTAAGCCAACGCTAGATATATGCAATGGTGATTATTTAGCTGAATTCTTCGACTTTGGCAAGCATCTTTCATCGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1158

Amino Acids

132.88

Weight (kDa)

9.19

Isoelectric Point (pI)

51.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000158)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10163
malus_domestica MD04G1068000.v1.1 MD05G1130900.v1.1 MD06G1153600.v1.1 MD10G1255900.v1.1 MD10G1282000.v1.1 MD13G1075400.v1.1 MD13G1192400.v1.1
pyrus_communis pycom01g00130 pycom01g00390 pycom01g00700 pycom01g00720 pycom01g00750 pycom01g00850 pycom01g00870 pycom01g00910 pycom01g01250 pycom01g01560 pycom01g01680 pycom01g01860 pycom01g01900 pycom01g01910 pycom01g02200 pycom01g02260 pycom01g02270 pycom01g02280 pycom01g02330 pycom01g02480 pycom01g02720 pycom01g02730 pycom01g02750 pycom01g02770 pycom01g02780 pycom01g02860 pycom01g02910 pycom01g03540 pycom01g04090 pycom01g04100 pycom01g04150 pycom01g04250 pycom01g04430 pycom01g11810 pycom01g11860 pycom01g11940 pycom01g21380 pycom02g06650 pycom02g08490 pycom02g08600 pycom02g18230 pycom02g18450 pycom02g18800 pycom02g19150 pycom02g20740 pycom02g21340 pycom02g26210 pycom03g12060 pycom03g19060 pycom03g19100 pycom03g19120 pycom04g06890 pycom04g08960 pycom05g01790 pycom05g07910 pycom05g08080 pycom05g08100 pycom05g08570 pycom05g21500 pycom05g22400 pycom05g22510 pycom05g22620 pycom05g23020 pycom05g23720 pycom05g23890 pycom05g24040 pycom07g12080 pycom08g07930 pycom08g11940 pycom09g12120 pycom10g03890 pycom10g23360 pycom10g28250 pycom11g02850 pycom11g14370 pycom11g14400 pycom11g21840 pycom11g21860 pycom11g21980 pycom11g23580 pycom11g23650 pycom11g24050 pycom11g25610 pycom12424g00060 pycom1256g00130 pycom12661g00030 pycom12g09520 pycom12g09530 pycom12g09660 pycom12g09730 pycom12g11210 pycom12g16710 pycom12g16740 pycom12g16750 pycom13g08800 pycom13g18580 pycom13g18670 pycom13g18770 pycom13g22900 pycom13g23050 pycom13g23160 pycom13g23480 pycom13g23610 pycom13g23660 pycom13g23790 pycom13g23840 pycom13g24010 pycom13g24140 pycom13g24210 pycom13g24300 pycom13g24310 pycom13g24480 pycom13g24520 pycom13g24570 pycom13g24730 pycom13g24760 pycom13g24820 pycom13g25010 pycom13g25020 pycom13g25270 pycom13g25620 pycom13g25670 pycom13g25760 pycom13g25800 pycom13g25900 pycom13g26620 pycom13g26640 pycom13g26670 pycom13g26940 pycom13g27040 pycom13g27130 pycom13g27530 pycom13g27770 pycom13g27830 pycom13g27920 pycom13g27940 pycom13g28100 pycom13g28420 pycom13g28430 pycom13g28460 pycom13g28520 pycom13g29160 pycom14g05380 pycom15g03480 pycom15g04370 pycom15g27500 pycom16g13590 pycom16g13610 pycom16g13630 pycom16g14390 pycom16g18310 pycom17g01940 pycom17g04000 pycom17g16100 pycom17g16220 pycom17g16230 pycom17g16340 pycom17g16430 pycom17g16460 pycom17g16740 pycom17g16960 pycom17g17020 pycom17g17090 pycom17g17100 pycom17g17170 pycom17g17430 pycom17g17850 pycom17g18230 pycom17g18250 pycom17g18380 pycom17g21170 pycom17g21330 pycom520g00150 pycom520g00160 pycom808g00030 pycom808g00160 pycom808g00250 pycom808g00290
rosa_chinensis RchiOBHm_Chr5g0054451

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 993
AasI GACNNNNNNGTC 1 cut(s) 835
Acc36I ACCTGC 3 cut(s) 14, 686, 1571
Acc65I GGTACC 1 cut(s) 704
AccB1I GGYRCC 2 cut(s) 686, 704
AccB7I CCANNNNNTGG 1 cut(s) 1869
AccBSI CCGCTC 2 cut(s) 298, 2887
AccI GTMKAC 3 cut(s) 1788, 2942, 2973
AccII CGCG 1 cut(s) 3390
AciI CCGC 7 cut(s) 20, 38, 296, 324, 683, 1402, 2885
AclWI GGATC 6 cut(s) 209, 518, 1900, 1919, 1932, 2486
AcoI YGGCCR 1 cut(s) 2204
AcuI CTGAAG 1 cut(s) 415
AcvI CACGTG 3 cut(s) 1630, 2444, 2865
AcyI GRCGYC 3 cut(s) 687, 2095, 2268
AdeI CACNNNGTG 1 cut(s) 1273
AfaI GTAC 7 cut(s) 706, 1168, 1199, 1234, 3002, 3095, 3134
AfiI CCNNNNNNNGG 9 cut(s) 245, 246, 247, 279, 399, 1299, 1869, 1921, 2987
AflIII ACRYGT 7 cut(s) 1163, 1627, 1629, 2034, 2443, 2519, 2864
AgsI TTSAA 7 cut(s) 568, 623, 723, 1817, 2060, 2816, 3070
AhdI GACNNNNNGTC 1 cut(s) 3268
AjiI CACGTC 2 cut(s) 955, 1164
AjnI CCWGG 5 cut(s) 278, 380, 1919, 2426, 2967
AjuI GAANNNNNNNTTGG 4 cut(s) 1921, 1953, 2099, 2131
Alw21I GWGCWC 5 cut(s) 156, 465, 2455, 2585, 3253
Alw26I GTCTC 4 cut(s) 634, 784, 1020, 1281
Alw44I GTGCAC 2 cut(s) 2451, 3249
AlwI GGATC 6 cut(s) 209, 518, 1900, 1919, 1932, 2486
AlwNI CAGNNNCTG 1 cut(s) 2572
Ama87I CYCGRG 6 cut(s) 90, 289, 765, 1728, 2018, 2421
ApaI GGGCCC 3 cut(s) 253, 296, 1247
ApaLI GTGCAC 2 cut(s) 2451, 3249
ApeKI GCWGC 2 cut(s) 8, 2460
ArsI GACNNNNNNTTYG 2 cut(s) 1139, 1171
Asp700I GAANNNNTTC 1 cut(s) 3041
Asp718I GGTACC 1 cut(s) 704
AspA2I CCTAGG 1 cut(s) 1120
AspLEI GCGC 5 cut(s) 689, 1224, 2897, 3390, 3392
AsuC2I CCSGG 7 cut(s) 91, 92, 290, 291, 1729, 1730, 2982
AsuII TTCGAA 1 cut(s) 2729
AsuNHI GCTAGC 1 cut(s) 4
AvaI CYCGRG 6 cut(s) 90, 289, 765, 1728, 2018, 2421
AvaII GGWCC 2 cut(s) 2073, 2162
AvrII CCTAGG 1 cut(s) 1120
AxyI CCTNAGG 2 cut(s) 211, 244
BaeGI GKGCMC 5 cut(s) 253, 296, 1247, 2455, 3253
BaeI ACNNNNGTAYC 2 cut(s) 1199, 1232
BalI TGGCCA 1 cut(s) 2206
BamHI GGATCC 1 cut(s) 1924
BanI GGYRCC 2 cut(s) 686, 704
BanII GRGCYC 5 cut(s) 156, 253, 296, 465, 1247
BauI CACGAG 3 cut(s) 167, 369, 476
BbrPI CACGTG 3 cut(s) 1630, 2444, 2865
BbsI GAAGAC 2 cut(s) 2203, 2920
Bbv12I GWGCWC 5 cut(s) 156, 465, 2455, 2585, 3253
BbvCI CCTCAGC 1 cut(s) 691
BbvI GCAGC 1 cut(s) 2472
BccI CCATC 9 cut(s) 176, 402, 485, 742, 1158, 1433, 1453, 2215, 3365
BceAI ACGGC 3 cut(s) 1520, 1707, 2723
BcgI CGANNNNNNTGC 2 cut(s) 2998, 3032
BciT130I CCWGG 5 cut(s) 280, 382, 1921, 2428, 2969
BcnI CCSGG 7 cut(s) 91, 92, 290, 291, 1729, 1730, 2982
BcoDI GTCTC 4 cut(s) 634, 784, 1020, 1281
BfoI RGCGCY 1 cut(s) 690
BfuAI ACCTGC 3 cut(s) 14, 686, 1571
BglI GCCNNNNNGGC 1 cut(s) 1407
BisI GCNGC 4 cut(s) 9, 21, 1402, 2461
BlnI CCTAGG 1 cut(s) 1120
BlsI GCNGC 4 cut(s) 10, 22, 1403, 2462
BmcAI AGTACT 1 cut(s) 1199
Bme18I GGWCC 2 cut(s) 2073, 2162
BmeRI GACNNNNNGTC 1 cut(s) 3268
BmeT110I CYCGRG 6 cut(s) 90, 289, 765, 1728, 2018, 2421
BmgBI CACGTC 2 cut(s) 955, 1164
BmrI ACTGGG 1 cut(s) 1467
BmsI GCATC 3 cut(s) 910, 2578, 3466
BmtI GCTAGC 1 cut(s) 8
BmuI ACTGGG 1 cut(s) 1467
BpiI GAAGAC 2 cut(s) 2203, 2920
Bpu10I CCTNAGC 2 cut(s) 691, 2081
Bpu14I TTCGAA 1 cut(s) 2729
BpuEI CTTGAG 1 cut(s) 2770
BpuMI CCSGG 7 cut(s) 91, 92, 290, 291, 1729, 1730, 2982
BsaAI YACGTR 4 cut(s) 1630, 2444, 2522, 2865
BsaBI GATNNNNATC 2 cut(s) 1591, 2190
BsaHI GRCGYC 3 cut(s) 687, 2095, 2268
BsaI GGTCTC 1 cut(s) 1020
BsaJI CCNNGG 8 cut(s) 90, 289, 519, 1120, 1728, 1920, 1928, 2427
BsaWI WCCGGW 2 cut(s) 1771, 1908
BsaXI ACNNNNNCTCC 4 cut(s) 1100, 1130, 2955, 2985
Bsc4I CCNNNNNNNGG 9 cut(s) 245, 246, 247, 279, 399, 1299, 1869, 1921, 2987
Bse1I ACTGG 9 cut(s) 147, 456, 1473, 1870, 1952, 2405, 2433, 2562, 3004
Bse21I CCTNAGG 2 cut(s) 211, 244
Bse3DI GCAATG 4 cut(s) 930, 1099, 3021, 3424
Bse8I GATNNNNATC 2 cut(s) 1591, 2190
BseBI CCWGG 5 cut(s) 280, 382, 1921, 2428, 2969
BseDI CCNNGG 8 cut(s) 90, 289, 519, 1120, 1728, 1920, 1928, 2427
BseGI GGATG 8 cut(s) 187, 230, 496, 539, 2207, 2779, 2830, 3039
BseJI GATNNNNATC 2 cut(s) 1591, 2190
BseLI CCNNNNNNNGG 9 cut(s) 245, 246, 247, 279, 399, 1299, 1869, 1921, 2987
BseMI GCAATG 4 cut(s) 930, 1099, 3021, 3424
BseMII CTCAG 4 cut(s) 705, 2060, 2792, 3162
BseNI ACTGG 9 cut(s) 147, 456, 1473, 1870, 1952, 2405, 2433, 2562, 3004
BsePI GCGCGC 1 cut(s) 3388
BseRI GAGGAG 4 cut(s) 1248, 1323, 3256, 3313
BseSI GKGCMC 5 cut(s) 253, 296, 1247, 2455, 3253
BseXI GCAGC 1 cut(s) 2472
BsgI GTGCAG 1 cut(s) 1549
Bsh1236I CGCG 1 cut(s) 3390
Bsh1285I CGRYCG 1 cut(s) 1772
BshNI GGYRCC 2 cut(s) 686, 704
BsiEI CGRYCG 1 cut(s) 1772
BsiHKAI GWGCWC 5 cut(s) 156, 465, 2455, 2585, 3253
BsiHKCI CYCGRG 6 cut(s) 90, 289, 765, 1728, 2018, 2421
BsiSI CCGG 6 cut(s) 91, 290, 1729, 1772, 1909, 2981
BslFI GGGAC 1 cut(s) 1560
BslI CCNNNNNNNGG 9 cut(s) 245, 246, 247, 279, 399, 1299, 1869, 1921, 2987
BsmAI GTCTC 4 cut(s) 634, 784, 1020, 1281
BsmFI GGGAC 1 cut(s) 1560
BsmI GAATGC 2 cut(s) 3016, 3105
Bso31I GGTCTC 1 cut(s) 1020
BsoBI CYCGRG 6 cut(s) 90, 289, 765, 1728, 2018, 2421
Bsp119I TTCGAA 1 cut(s) 2729
Bsp120I GGGCCC 3 cut(s) 249, 292, 1243
Bsp1286I GDGCHC 8 cut(s) 156, 253, 296, 465, 1247, 2455, 2585, 3253
Bsp1407I TGTACA 1 cut(s) 1166
Bsp143I GATC 6 cut(s) 201, 510, 1547, 1905, 1924, 2478
BspACI CCGC 7 cut(s) 20, 38, 296, 324, 683, 1402, 2885
BspCNI CTCAG 4 cut(s) 704, 2061, 2793, 3161
BspFNI CGCG 1 cut(s) 3390
BspMI ACCTGC 3 cut(s) 14, 686, 1571
BspOI GCTAGC 1 cut(s) 8
BspPI GGATC 6 cut(s) 209, 518, 1900, 1919, 1932, 2486
BspT104I TTCGAA 1 cut(s) 2729
BspT107I GGYRCC 2 cut(s) 686, 704
BspTNI GGTCTC 1 cut(s) 1020
BsrBI CCGCTC 2 cut(s) 298, 2887
BsrDI GCAATG 4 cut(s) 930, 1099, 3021, 3424
BsrGI TGTACA 1 cut(s) 1166
BsrI ACTGG 9 cut(s) 147, 456, 1473, 1870, 1952, 2405, 2433, 2562, 3004
BssECI CCNNGG 8 cut(s) 90, 289, 519, 1120, 1728, 1920, 1928, 2427
BssHII GCGCGC 1 cut(s) 3388
BssMI GATC 6 cut(s) 201, 510, 1547, 1905, 1924, 2478
BssNAI GTATAC 1 cut(s) 1789
BssNI GRCGYC 3 cut(s) 687, 2095, 2268
BssSI CACGAG 3 cut(s) 167, 369, 476
BssT1I CCWWGG 3 cut(s) 519, 1120, 1928
Bst1107I GTATAC 1 cut(s) 1789
Bst2BI CACGAG 3 cut(s) 167, 369, 476
Bst2UI CCWGG 5 cut(s) 280, 382, 1921, 2428, 2969
Bst4CI ACNGT 8 cut(s) 308, 350, 1134, 1186, 1903, 2044, 2287, 2320
Bst6I CTCTTC 1 cut(s) 1325
BstACI GRCGYC 3 cut(s) 687, 2095, 2268
BstAUI TGTACA 1 cut(s) 1166
BstBAI YACGTR 4 cut(s) 1630, 2444, 2522, 2865
BstBI TTCGAA 1 cut(s) 2729
BstC8I GCNNGC 6 cut(s) 6, 296, 685, 1412, 3390, 3455
BstEII GGTNACC 1 cut(s) 388
BstF5I GGATG 8 cut(s) 187, 230, 496, 539, 2207, 2779, 2830, 3039
BstFNI CGCG 1 cut(s) 3390
BstH2I RGCGCY 1 cut(s) 690
BstHHI GCGC 5 cut(s) 689, 1224, 2897, 3390, 3392
BstKTI GATC 6 cut(s) 204, 513, 1550, 1908, 1927, 2481
BstMAI GTCTC 4 cut(s) 634, 784, 1020, 1281
BstMBI GATC 6 cut(s) 201, 510, 1547, 1905, 1924, 2478
BstMCI CGRYCG 1 cut(s) 1772
BstMWI GCNNNNNNNGC 3 cut(s) 17, 1407, 2466
BstNI CCWGG 5 cut(s) 280, 382, 1921, 2428, 2969
BstNSI RCATGY 1 cut(s) 2038
BstPI GGTNACC 1 cut(s) 388
BstSLI GKGCMC 5 cut(s) 253, 296, 1247, 2455, 3253
BstUI CGCG 1 cut(s) 3390
BstV1I GCAGC 1 cut(s) 2472
BstV2I GAAGAC 2 cut(s) 2203, 2920
BstX2I RGATCY 2 cut(s) 1924, 2478
BstYI RGATCY 2 cut(s) 1924, 2478
BstZ17I GTATAC 1 cut(s) 1789
Bsu36I CCTNAGG 2 cut(s) 211, 244
BtgZI GCGATG 3 cut(s) 231, 2441, 3450
BtrI CACGTC 2 cut(s) 955, 1164
BtsCI GGATG 8 cut(s) 187, 230, 496, 539, 2207, 2779, 2830, 3039
BtsI GCAGTG 1 cut(s) 750
BtsIMutI CAGTG 7 cut(s) 154, 463, 750, 1683, 1863, 1959, 2412
BveI ACCTGC 3 cut(s) 14, 686, 1571
Cac8I GCNNGC 6 cut(s) 6, 296, 685, 1412, 3390, 3455
CaiI CAGNNNCTG 1 cut(s) 2572
CfoI GCGC 5 cut(s) 689, 1224, 2897, 3390, 3392
Cfr9I CCCGGG 3 cut(s) 90, 289, 1728
CseI GACGC 3 cut(s) 2103, 2276, 3311
Csp6I GTAC 7 cut(s) 705, 1167, 1198, 1233, 3001, 3094, 3133
CspCI CAANNNNNGTGG 2 cut(s) 2752, 2787
CviQI GTAC 7 cut(s) 705, 1167, 1198, 1233, 3001, 3094, 3133
DinI GGCGCC 1 cut(s) 688
DpnI GATC 6 cut(s) 203, 512, 1549, 1907, 1926, 2480
DpnII GATC 6 cut(s) 201, 510, 1547, 1905, 1924, 2478
DraI TTTAAA 2 cut(s) 739, 1591
DraIII CACNNNGTG 1 cut(s) 1273
DrdI GACNNNNNNGTC 1 cut(s) 835
DriI GACNNNNNGTC 1 cut(s) 3268
DseDI GACNNNNNNGTC 1 cut(s) 835
EaeI YGGCCR 1 cut(s) 2204
Eam1104I CTCTTC 1 cut(s) 1325
Eam1105I GACNNNNNGTC 1 cut(s) 3268
EarI CTCTTC 1 cut(s) 1325
Ecl136II GAGCTC 2 cut(s) 154, 463
Eco130I CCWWGG 3 cut(s) 519, 1120, 1928
Eco147I AGGCCT 2 cut(s) 165, 474
Eco24I GRGCYC 5 cut(s) 156, 253, 296, 465, 1247
Eco31I GGTCTC 1 cut(s) 1020
Eco32I GATATC 1 cut(s) 1621
Eco47I GGWCC 2 cut(s) 2073, 2162
Eco53kI GAGCTC 2 cut(s) 154, 463
Eco57I CTGAAG 1 cut(s) 415
Eco72I CACGTG 3 cut(s) 1630, 2444, 2865
Eco81I CCTNAGG 2 cut(s) 211, 244
Eco88I CYCGRG 6 cut(s) 90, 289, 765, 1728, 2018, 2421
Eco91I GGTNACC 1 cut(s) 388
EcoICRI GAGCTC 2 cut(s) 154, 463
EcoO109I RGGNCCY 5 cut(s) 31, 249, 1243, 2073, 2162
EcoO65I GGTNACC 1 cut(s) 388
EcoRI GAATTC 1 cut(s) 3437
EcoRII CCWGG 5 cut(s) 278, 380, 1919, 2426, 2967
EcoRV GATATC 1 cut(s) 1621
EcoT14I CCWWGG 3 cut(s) 519, 1120, 1928
EcoT22I ATGCAT 1 cut(s) 803
EcoT38I GRGCYC 5 cut(s) 156, 253, 296, 465, 1247
EgeI GGCGCC 1 cut(s) 688
EheI GGCGCC 1 cut(s) 688
ErhI CCWWGG 3 cut(s) 519, 1120, 1928
FaqI GGGAC 1 cut(s) 1560
FauI CCCGC 2 cut(s) 303, 676
FblI GTMKAC 3 cut(s) 1788, 2942, 2973
Fnu4HI GCNGC 4 cut(s) 9, 21, 1402, 2461
FokI GGATG 8 cut(s) 194, 237, 503, 546, 2194, 2766, 2817, 3046
FriOI GRGCYC 5 cut(s) 156, 253, 296, 465, 1247
Fsp4HI GCNGC 4 cut(s) 9, 21, 1402, 2461
GlaI GCGC 5 cut(s) 688, 1223, 2896, 3389, 3391
GluI GCNGC 4 cut(s) 9, 21, 1402, 2461
HaeII RGCGCY 1 cut(s) 690
HapII CCGG 6 cut(s) 91, 290, 1729, 1772, 1909, 2981
HgaI GACGC 3 cut(s) 2103, 2276, 3311
HhaI GCGC 5 cut(s) 689, 1224, 2897, 3390, 3392
Hin1I GRCGYC 3 cut(s) 687, 2095, 2268
Hin6I GCGC 5 cut(s) 687, 1222, 2895, 3388, 3390
HinP1I GCGC 5 cut(s) 687, 1222, 2895, 3388, 3390
HincII GTYRAC 7 cut(s) 1137, 1147, 1455, 2005, 2040, 2047, 2943
HindII GTYRAC 7 cut(s) 1137, 1147, 1455, 2005, 2040, 2047, 2943
HindIII AAGCTT 2 cut(s) 1811, 3121
HpaI GTTAAC 2 cut(s) 1455, 2005
HpaII CCGG 6 cut(s) 91, 290, 1729, 1772, 1909, 2981
Hpy188I TCNGA 9 cut(s) 125, 434, 1504, 1597, 1618, 1763, 2540, 2963, 3111
Hpy188III TCNNGA 7 cut(s) 369, 767, 789, 1192, 1300, 2018, 3150
Hpy99I CGWCG 3 cut(s) 267, 2097, 3305
HpyAV CCTTC 7 cut(s) 118, 427, 1292, 1509, 1861, 1958, 2822
HpyCH4III ACNGT 8 cut(s) 308, 350, 1134, 1186, 1903, 2044, 2287, 2320
HpyCH4IV ACGT 9 cut(s) 256, 846, 954, 1163, 1629, 2443, 2521, 2597, 2864
HpyF10VI GCNNNNNNNGC 3 cut(s) 17, 1407, 2466
HpySE526I ACGT 9 cut(s) 256, 846, 954, 1163, 1629, 2443, 2521, 2597, 2864
Hsp92I GRCGYC 3 cut(s) 687, 2095, 2268
HspAI GCGC 5 cut(s) 687, 1222, 2895, 3388, 3390
KasI GGCGCC 1 cut(s) 686
KpnI GGTACC 1 cut(s) 708
KspAI GTTAAC 2 cut(s) 1455, 2005
Kzo9I GATC 6 cut(s) 201, 510, 1547, 1905, 1924, 2478
LmnI GCTCC 5 cut(s) 159, 303, 468, 1619, 2404
Lsp1109I GCAGC 1 cut(s) 2472
LweI GCATC 3 cut(s) 910, 2578, 3466
MaeII ACGT 9 cut(s) 256, 846, 954, 1163, 1629, 2443, 2521, 2597, 2864
MaeIII GTNAC 9 cut(s) 77, 227, 388, 536, 1159, 2515, 2716, 3337, 3346
MalI GATC 6 cut(s) 203, 512, 1549, 1907, 1926, 2480
MbiI CCGCTC 2 cut(s) 298, 2887
MboI GATC 6 cut(s) 201, 510, 1547, 1905, 1924, 2478
MflI RGATCY 2 cut(s) 1924, 2478
MhlI GDGCHC 8 cut(s) 156, 253, 296, 465, 1247, 2455, 2585, 3253
MlsI TGGCCA 1 cut(s) 2206
MluNI TGGCCA 1 cut(s) 2206
Mly113I GGCGCC 1 cut(s) 687
MlyI GAGTC 5 cut(s) 1514, 2030, 2193, 2973, 3257
MmeI TCCRAC 5 cut(s) 789, 1361, 1386, 1786, 3185
Mox20I TGGCCA 1 cut(s) 2206
Mph1103I ATGCAT 1 cut(s) 803
MroXI GAANNNNTTC 1 cut(s) 3041
MscI TGGCCA 1 cut(s) 2206
MslI CAYNNNNRTG 3 cut(s) 2446, 2532, 3368
Msp20I TGGCCA 1 cut(s) 2206
MspI CCGG 6 cut(s) 91, 290, 1729, 1772, 1909, 2981
Mva1269I GAATGC 2 cut(s) 3016, 3105
MvaI CCWGG 5 cut(s) 280, 382, 1921, 2428, 2969
MvnI CGCG 1 cut(s) 3390
MwoI GCNNNNNNNGC 3 cut(s) 17, 1407, 2466
NarI GGCGCC 1 cut(s) 687
NciI CCSGG 7 cut(s) 91, 92, 290, 291, 1729, 1730, 2982
NdeII GATC 6 cut(s) 201, 510, 1547, 1905, 1924, 2478
NheI GCTAGC 1 cut(s) 4
NmeAIII GCCGAG 4 cut(s) 65, 1181, 1498, 1569
NmuCI GTSAC 6 cut(s) 77, 227, 388, 536, 1159, 3337
NsiI ATGCAT 1 cut(s) 803
NspI RCATGY 1 cut(s) 2038
NspV TTCGAA 1 cut(s) 2729
PaeR7I CTCGAG 2 cut(s) 2018, 2421
PauI GCGCGC 1 cut(s) 3388
PceI AGGCCT 2 cut(s) 165, 474
PciI ACATGT 1 cut(s) 2034
PctI GAATGC 2 cut(s) 3016, 3105
PdmI GAANNNNTTC 1 cut(s) 3041
PfeI GAWTC 7 cut(s) 812, 1796, 2102, 2354, 2745, 2958, 3374
PflMI CCANNNNNTGG 1 cut(s) 1869
PkrI GCNGC 4 cut(s) 10, 22, 1403, 2462
PleI GAGTC 5 cut(s) 1513, 2029, 2193, 2972, 3257
PluTI GGCGCC 1 cut(s) 690
PmaCI CACGTG 3 cut(s) 1630, 2444, 2865
PmlI CACGTG 3 cut(s) 1630, 2444, 2865
PpsI GAGTC 5 cut(s) 1513, 2029, 2193, 2972, 3257
Ppu21I YACGTR 4 cut(s) 1630, 2444, 2522, 2865
PpuMI RGGWCCY 2 cut(s) 2073, 2162
PscI ACATGT 1 cut(s) 2034
PsiI TTATAA 1 cut(s) 993
Psp124BI GAGCTC 2 cut(s) 156, 465
Psp5II RGGWCCY 2 cut(s) 2073, 2162
Psp6I CCWGG 5 cut(s) 278, 380, 1919, 2426, 2967
PspCI CACGTG 3 cut(s) 1630, 2444, 2865
PspEI GGTNACC 1 cut(s) 388
PspGI CCWGG 5 cut(s) 278, 380, 1919, 2426, 2967
PspOMI GGGCCC 3 cut(s) 249, 292, 1243
PspPPI RGGWCCY 2 cut(s) 2073, 2162
PspXI VCTCGAGB 1 cut(s) 2421
PstNI CAGNNNCTG 1 cut(s) 2572
PsuI RGATCY 2 cut(s) 1924, 2478
PteI GCGCGC 1 cut(s) 3388
RsaI GTAC 7 cut(s) 706, 1168, 1199, 1234, 3002, 3095, 3134
RsaNI GTAC 7 cut(s) 705, 1167, 1198, 1233, 3001, 3094, 3133
RseI CAYNNNNRTG 3 cut(s) 2446, 2532, 3368
SacI GAGCTC 2 cut(s) 156, 465
SalI GTCGAC 1 cut(s) 2941
SatI GCNGC 4 cut(s) 9, 21, 1402, 2461
Sau3AI GATC 6 cut(s) 201, 510, 1547, 1905, 1924, 2478
ScaI AGTACT 1 cut(s) 1199
SchI GAGTC 5 cut(s) 1514, 2030, 2193, 2973, 3257
SduI GDGCHC 8 cut(s) 156, 253, 296, 465, 1247, 2455, 2585, 3253
SfaNI GCATC 3 cut(s) 910, 2578, 3466
SfoI GGCGCC 1 cut(s) 688
Sfr274I CTCGAG 2 cut(s) 2018, 2421
SfuI TTCGAA 1 cut(s) 2729
SinI GGWCC 2 cut(s) 2073, 2162
SlaI CTCGAG 2 cut(s) 2018, 2421
SmaI CCCGGG 3 cut(s) 92, 291, 1730
SmiI ATTTAAAT 1 cut(s) 1591
SmiMI CAYNNNNRTG 3 cut(s) 2446, 2532, 3368
SmlI CTYRAG 3 cut(s) 2018, 2421, 2785
SmoI CTYRAG 3 cut(s) 2018, 2421, 2785
SseBI AGGCCT 2 cut(s) 165, 474
SsiI CCGC 7 cut(s) 20, 38, 296, 324, 683, 1402, 2885
SspDI GGCGCC 1 cut(s) 686
SspI AATATT 7 cut(s) 756, 980, 1937, 1971, 2170, 2299, 2332
SstI GAGCTC 2 cut(s) 156, 465
StuI AGGCCT 2 cut(s) 165, 474
StyI CCWWGG 3 cut(s) 519, 1120, 1928
SwaI ATTTAAAT 1 cut(s) 1591
TaaI ACNGT 8 cut(s) 308, 350, 1134, 1186, 1903, 2044, 2287, 2320
TaiI ACGT 9 cut(s) 259, 849, 957, 1166, 1632, 2446, 2524, 2600, 2867
TatI WGTACW 2 cut(s) 1166, 1197
TauI GCSGC 2 cut(s) 23, 1404
TfiI GAWTC 7 cut(s) 812, 1796, 2102, 2354, 2745, 2958, 3374
TscAI CASTG 7 cut(s) 154, 463, 750, 1690, 1870, 1959, 2412
TseFI GTSAC 6 cut(s) 77, 227, 388, 536, 1159, 3337
TseI GCWGC 2 cut(s) 8, 2460
Tsp45I GTSAC 6 cut(s) 77, 227, 388, 536, 1159, 3337
TspGWI ACGGA 3 cut(s) 149, 458, 1984
TspMI CCCGGG 3 cut(s) 90, 289, 1728
TspRI CASTG 7 cut(s) 154, 463, 750, 1690, 1870, 1959, 2412
Van91I CCANNNNNTGG 1 cut(s) 1869
VneI GTGCAC 2 cut(s) 2451, 3249
VpaK11BI GGWCC 2 cut(s) 2073, 2162
XceI RCATGY 1 cut(s) 2038
XhoI CTCGAG 2 cut(s) 2018, 2421
XmaI CCCGGG 3 cut(s) 90, 289, 1728
XmaJI CCTAGG 1 cut(s) 1120
XmiI GTMKAC 3 cut(s) 1788, 2942, 2973
XmnI GAANNNNTTC 1 cut(s) 3041
ZrmI AGTACT 1 cut(s) 1199
Zsp2I ATGCAT 1 cut(s) 803
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.