FvH4_2g02050

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000129
Physical Location & Seq
Forward (+)
1722124 .. 1732362
10239 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g02050.t3

Sequence Viewer

Length: 3822 bp
ATGACCACCCAAACAGAGATTGCTTCTGCTTCTTCTTCTCCTCCTCCTCCGATCCCTCGATGGATATACGATGTCTTCCTAAGTTTCAGAGGCACAGACACCCGCAATAGTTTTACAGATTTTCTTTATAACACTCTGAAACAGAAAGGCATAAACACATTTAGGGATAATGAAGAACTTGAGAGGGGGAAAGAAATTGCTCCGGAACTCTTGAAGGCAATTGAAGAATCAAGATATGTAATTGTCGTTCTTTCAGAAAATTATGCCGATTCATCTTGGTGTTTGGATGAGGTTGCCAAGGTCGTGGAATGCATGAAACTGATGGGACAGACAGTAGTCCCAGTTTTCTATCATGTGGATCCGTTTGAGGTGCGAAGACAAAAAGGGAGTTTCGAGAAAGCTTTCGCCAAGCATGAAATACGATTGAAGGACAATCCAGAGAAGGTGAAAAGGTGGAGATCTGCTCTTACCGATGTAGCTAATCTCTCTGGATGGCATTTGAAAGATCGAGCTGAATCAGAAGTTGTGCAAGAGATTGTACGATACATTTTCAACAAACTAACTTATCAAACAATCTCAAGTCGTCCCTGCACGGAGTTAGTTGGCATAGATTCTCGAGTAAACGAACTCCTTGATTACTTGGATATGCATAACATGAATGGAGTATGTACCGTAGGCGTTTTGGGTATGGGGGGCATCGGTAAGACAACTATTGCCCAAGAAGTTTGTGACAGGATATACGGTCAGTTTGAGGCTTACAGCTTTGTTCCAAAAGTAAGAGAAGTATCTGGAAAAGAAGGGCTCGTTCATTTACAGAAGCAACTTATTTCCGACATCTTGATGGAATCTAATGTGAATATACAAAGTATTAAAAGGGGAATCGACATAATGAGCCAGAGATTTTGCACTAGGAGAGTTCTTATCATTCTTGATGACGTGGATGACTTGGAACAATTGAAAGCTCTGTGCGGTCATGGTTGGTTTGGCTCAGGGAGTAAAGTCATAATAACATCAAGGGATAGACAACTGCTGCTTACATATGGGATAAGAGAGGACCGTATATATAGTGTTAGGCTATTGAGTGATGTGGAAGCCCTTCAGCTGTTTAGTTGGAAATCCTTCAAGAAATATAAGGTCAAAGAAGATTTTATCCAGCTATCAAAGAAGGTTGTGGAATATGCTCATGGGCTTCCATTAGCTATTGAACTTCTGGGTTCCTTGCTCTTTGGTAAAGACATAAAAGTATGGACAAGTGCATTGGATAGACTAAAAGCAGGAAATCCTACCAAAAAAATTGTTAATGTGTATAAAATAAGTTTTGATGCCTTGGAAGACACAGAAAAGGAAGTATTTCTGGACATTGCATGTTTCTTTAAAGGAGAAGACAAAGATCGTGTAATAAGAATACTAGAAAGTAAGTCTGGCTACAGCCCAGACATAGATATAGAAGTTCTCATGGATAAATCTATAGTAAATCTCCTTGGAAGAAAAGTGGAGTTACATGATAGGATACAAGAACTGGGCTGGGACATTGTTCGTCGACAAAAGCCGTACAAAGGTAGTAGGTTGTGGCTTCTCAAGGATATCATTCATTTGCTCAAACATAACGAGGGCACGACTGCAATGGAAGCTATATTACTCGATTCGCCACAACCAGAGAATGTTCGCTTCAATGTTGATCCTTTCTCGAAGATGTCCAAGCTAAGATTGCTCAAAATTAAAAATGTTCACCTTTTTGGAGAGGTTAAATGTCTCTCTAATGAGTTGCAACTTCTCGAGTGGCATGTCTGTCCATTACGTTCTTTGCCGTCAGACTTCCAGTCTGATAAGCTTGTTGAACTTAGACTGCAGTCTAGCTCTATCAGAGTGCTATGGAAGGGGAATAAACAGTGGAGAATGCTAAAGTTCATTGATATGAGTTATTCCCAATACTTGATGAAGACCCCAGATTTCAGTGAAGTTCCAAATCTTGAGACGTTGATACTTGAAGGCTGTATAAGATTGGTTGAGGTTCACACATCCGTTGGAGTCCTTGAAAAACTTAGTTTATGGAATATGGGAGATTGCAAATCTGTTAAGAGCCTTCCACCATTCCTTCGGTTGGAGTCCCTTACAACTTTAAAGCTCTCCGCCTGCTCGAAGCTGAAGAAGTTTCCTAAAATCGAAGGATGTATGCAACGCTTGTCGGAGCTTCATCTAGATGGGACTGCCATAGAGGAATTACCTCAGTCGATTGATCGTCTGACTGGTCTTACTCTTTTGAATTTAGAAGACTGCAAAAATCTTTTTCTTATTCCTAGTACCATTCGATCCTTGAAATCTCTGAAACGTCTAATTCTTACAGGTTGCTCAGAACTTAGTGACATATCTGACGATCTGAGTTGTGTAGAATGTCTGGAGGAGCTTGATATCAGTGGAACTGCTATAAGAGATTTCACGGGCATCGAAGGAATGAAGAATCTGAAGTCCCTATCTCTAAGAGGGTCTAGAAACTCAACCCCAAAATCATGGCATTCGGCGGTCTTGGGTTGTTTGGGGATGGTTAAAAGCAGCGATGCTCCCTTGAGATTGTCACTGCCTACGTCCTTCTCAGGTTTATGTTCTTTGACAGAGCTAAACATAAATGATTGTAATCTGATGGATGGAGTGATACCCAATGATCTTGGCAATCTAGTCTTATTGAAGAGATTAAATCTGAGTGGTAATAACTTTGTTCATTTACCGGAAAGCATCTCTCAGCTCTCCAAGCTTGAATCTCTCTACCTGAGAAACTGTAGCAAGCTGCAATTACTGCCTAAGAACCTTCCATTGAGTCTTCGAGATGTGAATGCAGAAGATTGTCAGTCACTGACAGATTATCCAGATCAATTCAAAGTATGGACTTCCACTGTTTCAGGAATGACTACTATTAGTTCCATCATTTCATCCAAACATCAAACATATTCTGCTACTGGTCAGGCCGGGAAAATCTCACACACATGGATTGCATCTACTATCCCGGGACGTAGCATCTTCAGTTGTTGCACATCATCAGATAACCATCAACAACGGAAGCTAGTTGAGGATTTGTCGTTGCCAAATCAAGTGCTTCAGAAACAACTTGAGCTACTCGATTATAAGTCAAGCCCCATTAGTTCTGGTTGTCATGGAGATGAAATTCCCGAGTGGTTCAGCAATGTAGATTCAAACAGTTACATAGGACAACGCATACCTCCAGGACTGGAAGATGAGGAACAGTGGATGGGGGTTTCTGTTGTCATCTTAGTCAAGGGACATCCTCCTGTCTCGGAGATTGAACCGGAAACTTCTAACTATTTCTATAAATGCATAGTCGGAACTGAAGATTTTCAACTGAGTCCGATTCTCCTTGACTGGGACAAATATCTTGGCTCACTTCCATTCAGTTCTCATTTTCTTTTCTTTTTCTACGTACCTCGCCTCTACTTCTCCTCCAAGAATTTAAATCAATCACGTTTAATGTGCGCCTTCTTTTGGACCAATAACCCACGCATGGAGGTCCAGAAATGTGGGATTCGCCTACTATTTGAGCAAGGGATTCCAGAATTCATCCAAACATACATGCCACCTGCTTTTGGTCAAGACCGCAAACATAAGGTCATCTGCGAATTCCCTGCGTCTTCAGAAAGCGAAAATGTTGATGAAACTTCAATTGATTCTGGATGGTTAAGTCTACAAATTGGTGGCAATCTTAGATGGGAGAAACTAATTCCAACTTGTACAGAAGAAATTCAGTTGAGACGGAACATTGAGTCGGTTCTTTTAAGATATCTCCAGGGGATGCCCGGCGTGGTTCAACCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1274

Amino Acids

144.59

Weight (kDa)

6.35

Isoelectric Point (pI)

50.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 23 - 203 2.9e-58 TIR domain
TIR_2 PF13676 25 - 121 2.9e-16 TIR domain
NB-ARC PF00931 205 - 376 9e-19 NB-ARC domain
NACHT PF05729 224 - 368 6e-06 NACHT domain
WHD_ROQ1 PF23282 445 - 515 1.2e-15 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 691 - 847 2.3e-11 Leucine-rich repeat region
LRR_13 PF23286 701 - 788 3.8e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 863 - 934 2.1e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 129, 3117
AarI CACCTGC 1 cut(s) 3595
Acc36I ACCTGC 1 cut(s) 3595
AccI GTMKAC 2 cut(s) 1540, 3691
AccIII TCCGGA 1 cut(s) 202
AciI CCGC 5 cut(s) 103, 969, 2130, 2519, 3604
AclWI GGATC 5 cut(s) 46, 353, 366, 1673, 2304
AcsI RAATTY 6 cut(s) 2263, 3156, 3457, 3563, 3626, 3747
AcuI CTGAAG 7 cut(s) 1082, 2165, 2483, 2998, 3074, 3360, 3624
AfaI GTAC 6 cut(s) 540, 670, 1553, 2302, 3432, 3739
AfiI CCNNNNNNNGG 6 cut(s) 1556, 2101, 3373, 3492, 3511, 3593
AhdI GACNNNNNGTC 1 cut(s) 1819
AjiI CACGTC 1 cut(s) 937
AjnI CCWGG 2 cut(s) 3214, 3792
Alw26I GTCTC 4 cut(s) 1757, 1967, 3289, 3751
AlwI GGATC 5 cut(s) 46, 353, 366, 1673, 2304
AlwNI CAGNNNCTG 1 cut(s) 2848
Ama87I CYCGRG 4 cut(s) 615, 1775, 2998, 3161
Aor13HI TCCGGA 1 cut(s) 202
AoxI GGCC 1 cut(s) 2958
ApeKI GCWGC 3 cut(s) 1030, 2550, 2782
ApoI RAATTY 6 cut(s) 2263, 3156, 3457, 3563, 3626, 3747
ArsI GACNNNNNNTTYG 2 cut(s) 3600, 3632
Asp700I GAANNNNTTC 6 cut(s) 401, 1095, 1118, 1350, 3345, 3747
AspLEI GCGC 1 cut(s) 3485
AspS9I GGNCC 3 cut(s) 1054, 3495, 3517
AsuC2I CCSGG 4 cut(s) 2962, 2999, 3000, 3804
AsuHPI GGTGA 2 cut(s) 457, 1721
AvaI CYCGRG 4 cut(s) 615, 1775, 2998, 3161
AvaII GGWCC 3 cut(s) 1054, 3495, 3517
BaeGI GKGCMC 1 cut(s) 1616
BamHI GGATCC 1 cut(s) 358
BanII GRGCYC 1 cut(s) 804
BarI GAAGNNNNNNTAC 2 cut(s) 1620, 1652
BbsI GAAGAC 8 cut(s) 67, 382, 1338, 1389, 1946, 2277, 2807, 3630
BbvI GCAGC 3 cut(s) 1017, 2562, 2769
BceAI ACGGC 2 cut(s) 1534, 1792
BcgI CGANNNNNNTGC 2 cut(s) 3614, 3648
BciT130I CCWGG 2 cut(s) 3216, 3794
BciVI GTATCC 1 cut(s) 1503
BcnI CCSGG 4 cut(s) 2962, 2999, 3000, 3804
BcoDI GTCTC 4 cut(s) 1757, 1967, 3289, 3751
BfaI CTAG 8 cut(s) 909, 1409, 1854, 2198, 2298, 2487, 2672, 3056
BfmI CTRYAG 4 cut(s) 1426, 1467, 1847, 2773
BfuAI ACCTGC 1 cut(s) 3595
BfuI GTATCC 1 cut(s) 1503
BglII AGATCT 1 cut(s) 458
BisI GCNGC 3 cut(s) 1031, 2551, 2783
BlsI GCNGC 3 cut(s) 1032, 2552, 2784
Bme1390I CCNGG 6 cut(s) 2962, 2999, 3000, 3216, 3794, 3804
Bme18I GGWCC 3 cut(s) 1054, 3495, 3517
BmeRI GACNNNNNGTC 1 cut(s) 1819
BmeT110I CYCGRG 4 cut(s) 615, 1775, 2998, 3161
BmgBI CACGTC 1 cut(s) 937
BmgT120I GGNCC 3 cut(s) 1054, 3495, 3517
BmiI GGNNCC 2 cut(s) 360, 1216
BmrFI CCNGG 6 cut(s) 2962, 2999, 3000, 3216, 3794, 3804
BmrI ACTGGG 3 cut(s) 335, 1529, 3382
BmsI GCATC 8 cut(s) 705, 1312, 2451, 2545, 2739, 2996, 3018, 3789
BmuI ACTGGG 3 cut(s) 335, 1529, 3382
BoxI GACNNNNGTC 2 cut(s) 335, 1849
BpiI GAAGAC 8 cut(s) 67, 382, 1338, 1389, 1946, 2277, 2807, 3630
BplI GAGNNNNNCTC 2 cut(s) 448, 480
BpmI CTGGAG 3 cut(s) 2417, 3198, 3776
Bpu10I CCTNAGC 1 cut(s) 988
BpuEI CTTGAG 6 cut(s) 200, 562, 1562, 1991, 2584, 3122
BpuMI CCSGG 4 cut(s) 2962, 2999, 3000, 3804
BsaAI YACGTR 1 cut(s) 3430
BsaBI GATNNNNATC 2 cut(s) 2631, 3039
BsaJI CCNNGG 5 cut(s) 297, 1326, 1480, 2998, 3793
BsaWI WCCGGW 3 cut(s) 202, 2722, 3298
BsaXI ACNNNNNCTCC 2 cut(s) 3434, 3464
Bsc4I CCNNNNNNNGG 6 cut(s) 1556, 2101, 3373, 3492, 3511, 3593
Bse1I ACTGG 7 cut(s) 341, 1524, 1819, 2251, 2956, 3225, 3377
Bse3DI GCAATG 3 cut(s) 1359, 1629, 3181
Bse8I GATNNNNATC 2 cut(s) 2631, 3039
BseAI TCCGGA 1 cut(s) 202
BseBI CCWGG 2 cut(s) 3216, 3794
BseDI CCNNGG 5 cut(s) 297, 1326, 1480, 2998, 3793
BseJI GATNNNNATC 2 cut(s) 2631, 3039
BseLI CCNNNNNNNGG 6 cut(s) 1556, 2101, 3373, 3492, 3511, 3593
BseMI GCAATG 3 cut(s) 1359, 1629, 3181
BseMII CTCAG 9 cut(s) 1002, 2240, 2364, 2369, 2604, 2687, 2750, 2756, 3344
BseNI ACTGG 7 cut(s) 341, 1524, 1819, 2251, 2956, 3225, 3377
BseRI GAGGAG 5 cut(s) 30, 33, 36, 2414, 3439
BseSI GKGCMC 1 cut(s) 1616
BseXI GCAGC 3 cut(s) 1017, 2562, 2769
BseYI CCCAGC 1 cut(s) 1524
BsgI GTGCAG 1 cut(s) 574
BshFI GGCC 1 cut(s) 2960
BsiHKCI CYCGRG 4 cut(s) 615, 1775, 2998, 3161
BsiSI CCGG 6 cut(s) 203, 2723, 2961, 2999, 3299, 3804
BslI CCNNNNNNNGG 6 cut(s) 1556, 2101, 3373, 3492, 3511, 3593
BsmAI GTCTC 4 cut(s) 1757, 1967, 3289, 3751
BsmBI CGTCTC 2 cut(s) 1967, 3751
BsmI GAATGC 4 cut(s) 314, 1902, 2512, 2833
BsnI GGCC 1 cut(s) 2960
BsoBI CYCGRG 4 cut(s) 615, 1775, 2998, 3161
Bsp1286I GDGCHC 2 cut(s) 804, 1616
Bsp13I TCCGGA 1 cut(s) 202
Bsp1407I TGTACA 1 cut(s) 3737
BspACI CCGC 5 cut(s) 103, 969, 2130, 2519, 3604
BspANI GGCC 1 cut(s) 2960
BspCNI CTCAG 9 cut(s) 1001, 2239, 2363, 2370, 2603, 2688, 2749, 2757, 3345
BspEI TCCGGA 1 cut(s) 202
BspLI GGNNCC 2 cut(s) 360, 1216
BspMAI CTGCAG 1 cut(s) 1851
BspMI ACCTGC 1 cut(s) 3595
BspPI GGATC 5 cut(s) 46, 353, 366, 1673, 2304
BsrDI GCAATG 3 cut(s) 1359, 1629, 3181
BsrGI TGTACA 1 cut(s) 3737
BsrI ACTGG 7 cut(s) 341, 1524, 1819, 2251, 2956, 3225, 3377
BssECI CCNNGG 5 cut(s) 297, 1326, 1480, 2998, 3793
BssT1I CCWWGG 3 cut(s) 297, 1326, 1480
Bst2UI CCWGG 2 cut(s) 3216, 3794
Bst4CI ACNGT 9 cut(s) 334, 673, 743, 1058, 1890, 2774, 2890, 3191, 3237
Bst6I CTCTTC 1 cut(s) 2678
BstAPI GCANNNNNTGC 1 cut(s) 2791
BstAUI TGTACA 1 cut(s) 3737
BstBAI YACGTR 1 cut(s) 3430
BstC8I GCNNGC 2 cut(s) 2134, 2780
BstHHI GCGC 1 cut(s) 3485
BstMAI GTCTC 4 cut(s) 1757, 1967, 3289, 3751
BstMWI GCNNNNNNNGC 4 cut(s) 1628, 1708, 2746, 2791
BstNI CCWGG 2 cut(s) 3216, 3794
BstNSI RCATGY 3 cut(s) 1368, 1787, 3583
BstPAI GACNNNNGTC 2 cut(s) 335, 1849
BstSCI CCNGG 6 cut(s) 2960, 2997, 2998, 3214, 3792, 3802
BstSFI CTRYAG 4 cut(s) 1426, 1467, 1847, 2773
BstSLI GKGCMC 1 cut(s) 1616
BstSNI TACGTA 1 cut(s) 3430
BstV1I GCAGC 3 cut(s) 1017, 2562, 2769
BstV2I GAAGAC 8 cut(s) 67, 382, 1338, 1389, 1946, 2277, 2807, 3630
BstX2I RGATCY 2 cut(s) 358, 458
BstXI CCANNNNNNTGG 3 cut(s) 304, 2508, 3527
BstYI RGATCY 2 cut(s) 358, 458
BsuI GTATCC 1 cut(s) 1503
BsuRI GGCC 1 cut(s) 2960
BtgZI GCGATG 1 cut(s) 2568
BtrI CACGTC 1 cut(s) 937
BtsI GCAGTG 1 cut(s) 2573
BtsIMutI CAGTG 7 cut(s) 1895, 1960, 2419, 2573, 2846, 2886, 3242
BveI ACCTGC 1 cut(s) 3595
Cac8I GCNNGC 2 cut(s) 2134, 2780
CaiI CAGNNNCTG 1 cut(s) 2848
CfoI GCGC 1 cut(s) 3485
Cfr13I GGNCC 3 cut(s) 1054, 3495, 3517
Cfr9I CCCGGG 1 cut(s) 2998
CseI GACGC 1 cut(s) 3624
Csp6I GTAC 6 cut(s) 539, 669, 1552, 2301, 3431, 3738
CviQI GTAC 6 cut(s) 539, 669, 1552, 2301, 3431, 3738
DraI TTTAAA 3 cut(s) 1375, 2121, 3462
DriI GACNNNNNGTC 1 cut(s) 1819
Eam1104I CTCTTC 1 cut(s) 2678
Eam1105I GACNNNNNGTC 1 cut(s) 1819
EarI CTCTTC 1 cut(s) 2678
EciI GGCGGA 1 cut(s) 2119
Eco105I TACGTA 1 cut(s) 3430
Eco130I CCWWGG 3 cut(s) 297, 1326, 1480
Eco24I GRGCYC 1 cut(s) 804
Eco32I GATATC 3 cut(s) 1585, 2410, 3788
Eco47I GGWCC 3 cut(s) 1054, 3495, 3517
Eco57I CTGAAG 7 cut(s) 1082, 2165, 2483, 2998, 3074, 3360, 3624
Eco88I CYCGRG 4 cut(s) 615, 1775, 2998, 3161
EcoRI GAATTC 2 cut(s) 3563, 3626
EcoRII CCWGG 2 cut(s) 3214, 3792
EcoRV GATATC 3 cut(s) 1585, 2410, 3788
EcoT14I CCWWGG 3 cut(s) 297, 1326, 1480
EcoT22I ATGCAT 3 cut(s) 314, 651, 3329
EcoT38I GRGCYC 1 cut(s) 804
ErhI CCWWGG 3 cut(s) 297, 1326, 1480
Esp3I CGTCTC 2 cut(s) 1967, 3751
FauI CCCGC 1 cut(s) 110
FauNDI CATATG 1 cut(s) 1039
FblI GTMKAC 2 cut(s) 1540, 3691
Fnu4HI GCNGC 3 cut(s) 1031, 2551, 2783
FriOI GRGCYC 1 cut(s) 804
Fsp4HI GCNGC 3 cut(s) 1031, 2551, 2783
FspBI CTAG 8 cut(s) 909, 1409, 1854, 2198, 2298, 2487, 2672, 3056
GlaI GCGC 1 cut(s) 3484
GluI GCNGC 3 cut(s) 1031, 2551, 2783
GsaI CCCAGC 1 cut(s) 1528
GsuI CTGGAG 3 cut(s) 2417, 3198, 3776
HaeIII GGCC 1 cut(s) 2960
HapII CCGG 6 cut(s) 203, 2723, 2961, 2999, 3299, 3804
HgaI GACGC 1 cut(s) 3624
HhaI GCGC 1 cut(s) 3485
Hin6I GCGC 1 cut(s) 3483
HinP1I GCGC 1 cut(s) 3483
HincII GTYRAC 1 cut(s) 1541
HindII GTYRAC 1 cut(s) 1541
HindIII AAGCTT 3 cut(s) 399, 1829, 2747
HpaII CCGG 6 cut(s) 203, 2723, 2961, 2999, 3299, 3804
HphI GGTGA 2 cut(s) 457, 1721
Hpy166II GTNNAC 5 cut(s) 622, 1541, 1729, 2014, 3692
Hpy8I GTNNAC 5 cut(s) 622, 1541, 1729, 2014, 3692
Hpy99I CGWCG 1 cut(s) 1542
HpyCH4III ACNGT 9 cut(s) 334, 673, 743, 1058, 1890, 2774, 2890, 3191, 3237
HpyCH4IV ACGT 8 cut(s) 936, 1798, 1976, 2329, 2582, 3004, 3429, 3472
HpyF10VI GCNNNNNNNGC 4 cut(s) 1628, 1708, 2746, 2791
HpySE526I ACGT 8 cut(s) 936, 1798, 1976, 2329, 2582, 3004, 3429, 3472
HspAI GCGC 1 cut(s) 3483
Kpn2I TCCGGA 1 cut(s) 202
LmnI GCTCC 4 cut(s) 205, 2188, 2401, 2563
Lsp1109I GCAGC 3 cut(s) 1017, 2562, 2769
LweI GCATC 8 cut(s) 705, 1312, 2451, 2545, 2739, 2996, 3018, 3789
MaeI CTAG 8 cut(s) 909, 1409, 1854, 2198, 2298, 2487, 2672, 3056
MaeII ACGT 8 cut(s) 936, 1798, 1976, 2329, 2582, 3004, 3429, 3472
MaeIII GTNAC 6 cut(s) 728, 1497, 2360, 2571, 2844, 3191
MfeI CAATTG 3 cut(s) 219, 953, 3669
MflI RGATCY 2 cut(s) 358, 458
MhlI GDGCHC 2 cut(s) 804, 1616
MlyI GAGTC 5 cut(s) 2037, 2114, 2821, 3364, 3779
MmeI TCCRAC 7 cut(s) 855, 1091, 2005, 2082, 2166, 3313, 3755
Mph1103I ATGCAT 3 cut(s) 314, 651, 3329
MroI TCCGGA 1 cut(s) 202
MroXI GAANNNNTTC 6 cut(s) 401, 1095, 1118, 1350, 3345, 3747
MslI CAYNNNNRTG 6 cut(s) 277, 839, 1913, 2199, 2977, 3150
MspA1I CMGCKG 1 cut(s) 1102
MspI CCGG 6 cut(s) 203, 2723, 2961, 2999, 3299, 3804
MspR9I CCNGG 6 cut(s) 2962, 2999, 3000, 3216, 3794, 3804
MunI CAATTG 3 cut(s) 219, 953, 3669
Mva1269I GAATGC 4 cut(s) 314, 1902, 2512, 2833
MvaI CCWGG 2 cut(s) 3216, 3794
MwoI GCNNNNNNNGC 4 cut(s) 1628, 1708, 2746, 2791
NciI CCSGG 4 cut(s) 2962, 2999, 3000, 3804
NdeI CATATG 1 cut(s) 1039
NlaIV GGNNCC 2 cut(s) 360, 1216
NmuCI GTSAC 4 cut(s) 728, 2360, 2571, 2844
NsiI ATGCAT 3 cut(s) 314, 651, 3329
NspI RCATGY 3 cut(s) 1368, 1787, 3583
PaeR7I CTCGAG 2 cut(s) 615, 1775
PaqCI CACCTGC 1 cut(s) 3595
PctI GAATGC 4 cut(s) 314, 1902, 2512, 2833
PdmI GAANNNNTTC 6 cut(s) 401, 1095, 1118, 1350, 3345, 3747
PfoI TCCNGGA 1 cut(s) 3214
PkrI GCNGC 3 cut(s) 1032, 2552, 2784
PleI GAGTC 5 cut(s) 2036, 2113, 2820, 3363, 3778
PpsI GAGTC 5 cut(s) 2036, 2113, 2820, 3363, 3778
Ppu21I YACGTR 1 cut(s) 3430
PshAI GACNNNNGTC 2 cut(s) 335, 1849
PsiI TTATAA 2 cut(s) 129, 3117
Psp6I CCWGG 2 cut(s) 3214, 3792
PspFI CCCAGC 1 cut(s) 1524
PspGI CCWGG 2 cut(s) 3214, 3792
PspN4I GGNNCC 2 cut(s) 360, 1216
PspPI GGNCC 3 cut(s) 1054, 3495, 3517
PsrI GAACNNNNNNTAC 2 cut(s) 231, 263
PstI CTGCAG 1 cut(s) 1851
PstNI CAGNNNCTG 1 cut(s) 2848
PsuI RGATCY 2 cut(s) 358, 458
PvuII CAGCTG 1 cut(s) 1102
RsaI GTAC 6 cut(s) 540, 670, 1553, 2302, 3432, 3739
RsaNI GTAC 6 cut(s) 539, 669, 1552, 2301, 3431, 3738
RseI CAYNNNNRTG 6 cut(s) 277, 839, 1913, 2199, 2977, 3150
SalI GTCGAC 1 cut(s) 1539
SatI GCNGC 3 cut(s) 1031, 2551, 2783
Sau96I GGNCC 3 cut(s) 1054, 3495, 3517
SchI GAGTC 5 cut(s) 2037, 2114, 2821, 3364, 3779
ScrFI CCNGG 6 cut(s) 2962, 2999, 3000, 3216, 3794, 3804
SduI GDGCHC 2 cut(s) 804, 1616
SfaNI GCATC 8 cut(s) 705, 1312, 2451, 2545, 2739, 2996, 3018, 3789
SfcI CTRYAG 4 cut(s) 1426, 1467, 1847, 2773
Sfr274I CTCGAG 2 cut(s) 615, 1775
SinI GGWCC 3 cut(s) 1054, 3495, 3517
SlaI CTCGAG 2 cut(s) 615, 1775
SmaI CCCGGG 1 cut(s) 3000
SmiI ATTTAAAT 1 cut(s) 3462
SmiMI CAYNNNNRTG 6 cut(s) 277, 839, 1913, 2199, 2977, 3150
SmlI CTYRAG 8 cut(s) 179, 577, 615, 1577, 1775, 1970, 2563, 3101
SmoI CTYRAG 8 cut(s) 179, 577, 615, 1577, 1775, 1970, 2563, 3101
SnaBI TACGTA 1 cut(s) 3430
SsiI CCGC 5 cut(s) 103, 969, 2130, 2519, 3604
SspMI CTAG 8 cut(s) 909, 1409, 1854, 2198, 2298, 2487, 2672, 3056
StyD4I CCNGG 6 cut(s) 2960, 2997, 2998, 3214, 3792, 3802
StyI CCWWGG 3 cut(s) 297, 1326, 1480
SwaI ATTTAAAT 1 cut(s) 3462
TaaI ACNGT 9 cut(s) 334, 673, 743, 1058, 1890, 2774, 2890, 3191, 3237
TaiI ACGT 8 cut(s) 939, 1801, 1979, 2332, 2585, 3007, 3432, 3475
TatI WGTACW 1 cut(s) 3737
TscAI CASTG 7 cut(s) 1895, 1960, 2419, 2580, 2853, 2893, 3242
TseFI GTSAC 4 cut(s) 728, 2360, 2571, 2844
TseI GCWGC 3 cut(s) 1030, 2550, 2782
Tsp45I GTSAC 4 cut(s) 728, 2360, 2571, 2844
TspGWI ACGGA 5 cut(s) 351, 608, 2011, 3064, 3775
TspMI CCCGGG 1 cut(s) 2998
TspRI CASTG 7 cut(s) 1895, 1960, 2419, 2580, 2853, 2893, 3242
VpaK11BI GGWCC 3 cut(s) 1054, 3495, 3517
XapI RAATTY 6 cut(s) 2263, 3156, 3457, 3563, 3626, 3747
XbaI TCTAGA 2 cut(s) 2197, 2486
XceI RCATGY 3 cut(s) 1368, 1787, 3583
XhoI CTCGAG 2 cut(s) 615, 1775
XmaI CCCGGG 1 cut(s) 2998
XmiI GTMKAC 2 cut(s) 1540, 3691
XmnI GAANNNNTTC 6 cut(s) 401, 1095, 1118, 1350, 3345, 3747
XspI CTAG 8 cut(s) 909, 1409, 1854, 2198, 2298, 2487, 2672, 3056
Zsp2I ATGCAT 3 cut(s) 314, 651, 3329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.