MD05G1002100.v1.1

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000129
Physical Location & Seq
Forward (+)
951187 .. 952978
1792 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1002100.v1.1.491

Sequence Viewer

Length: 1287 bp
ATGGAAAAATCTCTACTAACTCTGTTCGGAAGAAAGTTGTGGATGCATGATTTGATACAAGAATTAGGCTGGGAGATTGTTCGTCGAGAATGCAGTGAAGAGCCGGGCAAACGTAGCAGGTTGTGGCTTCCCAAAGATATCATCCATGTACTTGTAAATGATGAGGGAACAATTGCGGTTGAAGGTATATTCTTGAATTTCCCAAAAGAAGAGGAGGTTTTCTGGAATGTTGATCCCTTCCCAAAGATGCCCAAGCTAAGATTGCTCAGAATTAGTAATGTGACATTTTCTGGGTGTGTCAAATATCTTTCAAATGAGTTACAGCTTTTGGAATGGCATGGATGTCCTTTAAGTTCTTTTCCATCAAACTTTCAATCAAATAATCTTGTTGAACTCATCATGCATTCGAGCTTTATTAAAGAACTATGGAGGGGAAATCAAAGTTGGAGAATGCTACAGTTTATTGATTTGAGTGGTTCCAAATACTTGATCAAGACCCCAAACTTCAGTGAGGCTCCAAATATTGAGACACTAGTGCTTCAAGATTGTACAAGATTGGTCGAGGTTCACCCCTCCATTGGAGTTCTCAAAAAACTTATTTTGTTGAATATGAGAAACTGCAAACGCGTTGAGAGCCTTCCACCTTCCATTAGCTTGGAATCTCTTGAGATTTTTATTCTTTCTGCCTGTTCGAGACTCAAGAAGTTTCCTGAAATTGGAGGATATATGGGAAACCTGTTGGAGCTTCATTTAGATGGAACTGCTATACAGGAATTACCTACGTCTATTGAGTGTTTGCTTGGCCTTACTTTGTTGAACTTACAAGACTGCAAAAACCTTTTGCATCTTCCAAGTACCATTCAGTATCTGACGTCTCTGAAATCTCTGAATCTATCAGGTTGCTCAGAGCTTGACGAGATGCCGGCAAATCTGAGTTCTGTGGAATGTCTGGAGAGGCTTGATATAAGTGGAACTGCAATACGAGAATCATCATTCATTCTAGATATGAAGAATCTACAGTTTCTTTCCTTGCAAGGATGTAAGGATCTTCCTCCAAAATCATGGCATTCACTCTTTAATTGTTGGTGGTGGGGTAAAAAGGTCCAAGGTCCGTTGAGTTTGTTGTTGCCGACTTCATTCTCGGGTTTAACTTCCTTAACAAAGCTAAACTTGAGTGATTGCAATCTGACAGAGGGAGAAGTTCCGGATGATCTTGGCAGCTTATTCTCCTTAGAAATCTTAAATTTAAGTGCTTCCTTTAAGTGTTCGACAAGTGAATGCGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

429

Amino Acids

48.41

Weight (kDa)

5.53

Isoelectric Point (pI)

59.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_13 PF23286 219 - 306 2.2e-12 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 245 - 342 1.4e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 875
Acc36I ACCTGC 1 cut(s) 108
AccII CGCG 1 cut(s) 627
AccIII TCCGGA 1 cut(s) 1204
AciI CCGC 2 cut(s) 176, 1281
AclWI GGATC 2 cut(s) 227, 1053
AcsI RAATTY 2 cut(s) 196, 1243
AcuI CTGAAG 1 cut(s) 490
AcyI GRCGYC 1 cut(s) 872
AfaI GTAC 3 cut(s) 150, 550, 856
AfiI CCNNNNNNNGG 2 cut(s) 578, 716
AflIII ACRYGT 1 cut(s) 625
AgsI TTSAA 8 cut(s) 182, 196, 312, 374, 392, 542, 607, 817
AhlI ACTAGT 1 cut(s) 532
AjuI GAANNNNNNNTTGG 2 cut(s) 427, 459
AluBI AGCT 8 cut(s) 256, 325, 411, 654, 745, 910, 1165, 1221
AluI AGCT 8 cut(s) 256, 325, 411, 654, 745, 910, 1165, 1221
Alw26I GTCTC 3 cut(s) 521, 688, 879
AlwI GGATC 2 cut(s) 227, 1053
AlwNI CAGNNNCTG 1 cut(s) 868
Ama87I CYCGRG 1 cut(s) 1141
Aor13HI TCCGGA 1 cut(s) 1204
AoxI GGCC 1 cut(s) 802
ApeKI GCWGC 1 cut(s) 1218
ApoI RAATTY 2 cut(s) 196, 1243
AspS9I GGNCC 2 cut(s) 1102, 1109
AsuC2I CCSGG 1 cut(s) 105
AsuHPI GGTGA 1 cut(s) 560
AvaI CYCGRG 1 cut(s) 1141
AvaII GGWCC 2 cut(s) 1102, 1109
BbvI GCAGC 1 cut(s) 1230
BccI CCATC 2 cut(s) 370, 749
BclI TGATCA 1 cut(s) 489
BcnI CCSGG 1 cut(s) 105
BcoDI GTCTC 3 cut(s) 521, 688, 879
BcuI ACTAGT 1 cut(s) 532
BfaI CTAG 2 cut(s) 533, 1001
BfmI CTRYAG 2 cut(s) 455, 1016
BfuAI ACCTGC 1 cut(s) 108
BisI GCNGC 1 cut(s) 1219
BlsI GCNGC 1 cut(s) 1220
Bme1390I CCNGG 1 cut(s) 105
Bme18I GGWCC 2 cut(s) 1102, 1109
BmeT110I CYCGRG 1 cut(s) 1141
BmgT120I GGNCC 2 cut(s) 1102, 1109
BmiI GGNNCC 2 cut(s) 478, 516
BmrFI CCNGG 1 cut(s) 105
BmsI GCATC 4 cut(s) 33, 237, 853, 909
BpmI CTGGAG 1 cut(s) 971
BpuEI CTTGAG 3 cut(s) 683, 686, 1192
BpuMI CCSGG 1 cut(s) 105
BsaBI GATNNNNATC 1 cut(s) 1182
BsaHI GRCGYC 1 cut(s) 872
BsaJI CCNNGG 1 cut(s) 1105
BsaWI WCCGGW 1 cut(s) 1204
Bsc4I CCNNNNNNNGG 2 cut(s) 578, 716
Bse118I RCCGGY 1 cut(s) 922
Bse8I GATNNNNATC 1 cut(s) 1182
BseAI TCCGGA 1 cut(s) 1204
BseDI CCNNGG 1 cut(s) 1105
BseGI GGATG 5 cut(s) 48, 141, 347, 1043, 1213
BseJI GATNNNNATC 1 cut(s) 1182
BseLI CCNNNNNNNGG 2 cut(s) 578, 716
BseMII CTCAG 3 cut(s) 280, 918, 923
BseRI GAGGAG 1 cut(s) 227
BseXI GCAGC 1 cut(s) 1230
BseYI CCCAGC 1 cut(s) 69
Bsh1236I CGCG 1 cut(s) 627
BshFI GGCC 1 cut(s) 804
BsiHKCI CYCGRG 1 cut(s) 1141
BsiSI CCGG 3 cut(s) 104, 923, 1205
BslI CCNNNNNNNGG 2 cut(s) 578, 716
BsmAI GTCTC 3 cut(s) 521, 688, 879
BsmBI CGTCTC 1 cut(s) 879
BsmI GAATGC 5 cut(s) 95, 403, 456, 1066, 1283
BsnI GGCC 1 cut(s) 804
BsoBI CYCGRG 1 cut(s) 1141
Bsp13I TCCGGA 1 cut(s) 1204
Bsp1407I TGTACA 1 cut(s) 548
Bsp143I GATC 4 cut(s) 232, 489, 1045, 1210
BspACI CCGC 2 cut(s) 176, 1281
BspANI GGCC 1 cut(s) 804
BspCNI CTCAG 3 cut(s) 279, 917, 924
BspEI TCCGGA 1 cut(s) 1204
BspFNI CGCG 1 cut(s) 627
BspLI GGNNCC 2 cut(s) 478, 516
BspMI ACCTGC 1 cut(s) 108
BspPI GGATC 2 cut(s) 227, 1053
BspQI GCTCTTC 1 cut(s) 93
BsrFI RCCGGY 1 cut(s) 922
BsrGI TGTACA 1 cut(s) 548
BssAI RCCGGY 1 cut(s) 922
BssECI CCNNGG 1 cut(s) 1105
BssMI GATC 4 cut(s) 232, 489, 1045, 1210
BssNI GRCGYC 1 cut(s) 872
BssT1I CCWWGG 1 cut(s) 1105
Bst4CI ACNGT 2 cut(s) 459, 1020
Bst6I CTCTTC 2 cut(s) 93, 204
BstACI GRCGYC 1 cut(s) 872
BstAUI TGTACA 1 cut(s) 548
BstC8I GCNNGC 1 cut(s) 924
BstDEI CTNAG 5 cut(s) 257, 266, 904, 932, 1231
BstF5I GGATG 5 cut(s) 48, 141, 347, 1043, 1213
BstFNI CGCG 1 cut(s) 627
BstKTI GATC 4 cut(s) 235, 492, 1048, 1213
BstMAI GTCTC 3 cut(s) 521, 688, 879
BstMBI GATC 4 cut(s) 232, 489, 1045, 1210
BstMWI GCNNNNNNNGC 3 cut(s) 114, 262, 633
BstSCI CCNGG 1 cut(s) 103
BstSFI CTRYAG 2 cut(s) 455, 1016
BstUI CGCG 1 cut(s) 627
BstV1I GCAGC 1 cut(s) 1230
BstX2I RGATCY 1 cut(s) 1045
BstXI CCANNNNNNTGG 2 cut(s) 655, 1062
BstYI RGATCY 1 cut(s) 1045
BsuRI GGCC 1 cut(s) 804
BtsCI GGATG 5 cut(s) 48, 141, 347, 1043, 1213
BtsI GCAGTG 1 cut(s) 100
BtsIMutI CAGTG 2 cut(s) 100, 514
BveI ACCTGC 1 cut(s) 108
Cac8I GCNNGC 1 cut(s) 924
CaiI CAGNNNCTG 1 cut(s) 868
Cfr10I RCCGGY 1 cut(s) 922
Cfr13I GGNCC 2 cut(s) 1102, 1109
Csp6I GTAC 3 cut(s) 149, 549, 855
CviAII CATG 5 cut(s) 47, 146, 338, 400, 1062
CviQI GTAC 3 cut(s) 149, 549, 855
DdeI CTNAG 5 cut(s) 257, 266, 904, 932, 1231
DpnI GATC 4 cut(s) 234, 491, 1047, 1212
DpnII GATC 4 cut(s) 232, 489, 1045, 1210
Eam1104I CTCTTC 2 cut(s) 93, 204
EarI CTCTTC 2 cut(s) 93, 204
Eco130I CCWWGG 1 cut(s) 1105
Eco32I GATATC 1 cut(s) 139
Eco47I GGWCC 2 cut(s) 1102, 1109
Eco57I CTGAAG 1 cut(s) 490
Eco88I CYCGRG 1 cut(s) 1141
EcoRV GATATC 1 cut(s) 139
EcoT14I CCWWGG 1 cut(s) 1105
EcoT22I ATGCAT 2 cut(s) 48, 405
ErhI CCWWGG 1 cut(s) 1105
Esp3I CGTCTC 1 cut(s) 879
FaeI CATG 5 cut(s) 50, 149, 341, 403, 1065
FalI AAGNNNNNCTT 2 cut(s) 1154, 1186
FatI CATG 5 cut(s) 46, 145, 337, 399, 1061
FbaI TGATCA 1 cut(s) 489
Fnu4HI GCNGC 1 cut(s) 1219
FokI GGATG 5 cut(s) 55, 128, 354, 1050, 1220
Fsp4HI GCNGC 1 cut(s) 1219
FspBI CTAG 2 cut(s) 533, 1001
GluI GCNGC 1 cut(s) 1219
GsaI CCCAGC 1 cut(s) 73
GsuI CTGGAG 1 cut(s) 971
HaeIII GGCC 1 cut(s) 804
HapII CCGG 3 cut(s) 104, 923, 1205
Hin1I GRCGYC 1 cut(s) 872
Hin1II CATG 5 cut(s) 50, 149, 341, 403, 1065
HinfI GANTC 5 cut(s) 659, 696, 889, 986, 1012
HpaII CCGG 3 cut(s) 104, 923, 1205
HphI GGTGA 1 cut(s) 560
Hpy166II GTNNAC 1 cut(s) 568
Hpy188I TCNGA 8 cut(s) 29, 269, 870, 879, 888, 907, 933, 1188
Hpy8I GTNNAC 1 cut(s) 568
Hpy99I CGWCG 1 cut(s) 87
HpyAV CCTTC 4 cut(s) 176, 247, 647, 654
HpyCH4III ACNGT 2 cut(s) 459, 1020
HpyCH4IV ACGT 3 cut(s) 112, 782, 872
HpyCH4V TGCA 9 cut(s) 46, 93, 403, 621, 831, 844, 977, 1033, 1182
HpyF10VI GCNNNNNNNGC 3 cut(s) 114, 262, 633
HpyF3I CTNAG 5 cut(s) 257, 266, 904, 932, 1231
HpySE526I ACGT 3 cut(s) 112, 782, 872
Hsp92I GRCGYC 1 cut(s) 872
Hsp92II CATG 5 cut(s) 50, 149, 341, 403, 1065
Kpn2I TCCGGA 1 cut(s) 1204
KroI GCCGGC 1 cut(s) 922
KroNI GCCGGC 1 cut(s) 924
Ksp22I TGATCA 1 cut(s) 489
Kzo9I GATC 4 cut(s) 232, 489, 1045, 1210
LguI GCTCTTC 1 cut(s) 93
LmnI GCTCC 2 cut(s) 520, 742
Lsp1109I GCAGC 1 cut(s) 1230
LweI GCATC 4 cut(s) 33, 237, 853, 909
MaeI CTAG 2 cut(s) 533, 1001
MaeII ACGT 3 cut(s) 112, 782, 872
MaeIII GTNAC 2 cut(s) 280, 318
MalI GATC 4 cut(s) 234, 491, 1047, 1212
MboI GATC 4 cut(s) 232, 489, 1045, 1210
MboII GAAGA 6 cut(s) 42, 110, 221, 839, 1021, 1040
MfeI CAATTG 1 cut(s) 171
MflI RGATCY 1 cut(s) 1045
MluCI AATT 8 cut(s) 62, 171, 196, 270, 714, 773, 1078, 1243
MluI ACGCGT 1 cut(s) 625
MlyI GAGTC 1 cut(s) 690
MmeI TCCRAC 2 cut(s) 425, 720
Mph1103I ATGCAT 2 cut(s) 48, 405
MroI TCCGGA 1 cut(s) 1204
MroNI GCCGGC 1 cut(s) 922
MseI TTAA 8 cut(s) 350, 417, 1077, 1148, 1157, 1241, 1247, 1260
MslI CAYNNNNRTG 1 cut(s) 753
MspI CCGG 3 cut(s) 104, 923, 1205
MspR9I CCNGG 1 cut(s) 105
MunI CAATTG 1 cut(s) 171
Mva1269I GAATGC 5 cut(s) 95, 403, 456, 1066, 1283
MvnI CGCG 1 cut(s) 627
MwoI GCNNNNNNNGC 3 cut(s) 114, 262, 633
NaeI GCCGGC 1 cut(s) 924
NciI CCSGG 1 cut(s) 105
NdeII GATC 4 cut(s) 232, 489, 1045, 1210
NgoMIV GCCGGC 1 cut(s) 922
NlaIII CATG 5 cut(s) 50, 149, 341, 403, 1065
NlaIV GGNNCC 2 cut(s) 478, 516
NmuCI GTSAC 1 cut(s) 280
NsiI ATGCAT 2 cut(s) 48, 405
PciSI GCTCTTC 1 cut(s) 93
PctI GAATGC 5 cut(s) 95, 403, 456, 1066, 1283
PdiI GCCGGC 1 cut(s) 924
PfeI GAWTC 4 cut(s) 659, 889, 986, 1012
PkrI GCNGC 1 cut(s) 1220
PleI GAGTC 1 cut(s) 690
PpsI GAGTC 1 cut(s) 690
PspFI CCCAGC 1 cut(s) 69
PspN4I GGNNCC 2 cut(s) 478, 516
PspPI GGNCC 2 cut(s) 1102, 1109
PstNI CAGNNNCTG 1 cut(s) 868
PsuI RGATCY 1 cut(s) 1045
RsaI GTAC 3 cut(s) 150, 550, 856
RsaNI GTAC 3 cut(s) 149, 549, 855
RseI CAYNNNNRTG 1 cut(s) 753
SapI GCTCTTC 1 cut(s) 93
SaqAI TTAA 8 cut(s) 350, 417, 1077, 1148, 1157, 1241, 1247, 1260
SatI GCNGC 1 cut(s) 1219
Sau3AI GATC 4 cut(s) 232, 489, 1045, 1210
Sau96I GGNCC 2 cut(s) 1102, 1109
SchI GAGTC 1 cut(s) 690
ScrFI CCNGG 1 cut(s) 105
SfaNI GCATC 4 cut(s) 33, 237, 853, 909
SfcI CTRYAG 2 cut(s) 455, 1016
SinI GGWCC 2 cut(s) 1102, 1109
SmiMI CAYNNNNRTG 1 cut(s) 753
SmlI CTYRAG 3 cut(s) 665, 698, 1171
SmoI CTYRAG 3 cut(s) 665, 698, 1171
SpeI ACTAGT 1 cut(s) 532
Sse9I AATT 8 cut(s) 62, 171, 196, 270, 714, 773, 1078, 1243
SsiI CCGC 2 cut(s) 176, 1281
SspI AATATT 1 cut(s) 523
SspMI CTAG 2 cut(s) 533, 1001
StyD4I CCNGG 1 cut(s) 103
StyI CCWWGG 1 cut(s) 1105
TaaI ACNGT 2 cut(s) 459, 1020
TaiI ACGT 3 cut(s) 115, 785, 875
TaqI TCGA 5 cut(s) 85, 407, 561, 692, 1268
TasI AATT 8 cut(s) 62, 171, 196, 270, 714, 773, 1078, 1243
TatI WGTACW 2 cut(s) 148, 548
TfiI GAWTC 4 cut(s) 659, 889, 986, 1012
Tru1I TTAA 8 cut(s) 350, 417, 1077, 1148, 1157, 1241, 1247, 1260
Tru9I TTAA 8 cut(s) 350, 417, 1077, 1148, 1157, 1241, 1247, 1260
TscAI CASTG 2 cut(s) 100, 514
TseFI GTSAC 1 cut(s) 280
TseI GCWGC 1 cut(s) 1218
Tsp45I GTSAC 1 cut(s) 280
TspDTI ATGAA 4 cut(s) 737, 985, 1022, 1125
TspGWI ACGGA 1 cut(s) 1101
TspRI CASTG 2 cut(s) 100, 514
VpaK11BI GGWCC 2 cut(s) 1102, 1109
XapI RAATTY 2 cut(s) 196, 1243
XbaI TCTAGA 1 cut(s) 1000
XspI CTAG 2 cut(s) 533, 1001
ZraI GACGTC 1 cut(s) 873
Zsp2I ATGCAT 2 cut(s) 48, 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.