Rw7G041460

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000129
Physical Location & Seq
Forward (+)
64740820 .. 64748026
7207 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G041460.1

Sequence Viewer

Length: 4998 bp
ATGGGCTTGTCGATCCGCCTCAGTGTCTTGTCTGCCACTTCCCTACTAGTTTTCTTCTCTGCTCTGTTTCTCTTTAACTTTCTCTCTTTATGGTTGTACTTCTTGTTCTTCTTCTCCACTGTTTGTATTTGCGGCTGCCTTTTGGCTTTCAAACTGAGACCAACAACACTCCCCAGTAGTTCTTCAGTCACTACCAAAGAAGATATTCCTTCTTCTTCTCCTCCATCCGGTAAATTGACGTACGATGTGTTCCTCAGTTTCAGCGGTAAAGACACCCGCAAAAATTTCACAGACCATCTTTACACTGCTCTCAAACAGAAAGGGATATACACATTTAGAGATGACGAAGAACTTGTGAGGGGGAAATCGATTGGTCCAAATCTCTTGAAAGGAATTGAAGAGTCAAGATATGTAATTGCTGTTTTCTCACGAAGTTACGCCGATTCTGCATGGTGTTTGGATGAGATTGCAAAGGTCGCTGAATGCATGAAACGGAAAGAAATGAAACAAATAGTCCTCCCAGTGTTCTATGATGTCGATCCATATGAGGTACGGAGACAAAAAGGCCAACACTTTGAGAAAGCATTTCAGAAGCATCAAAAGAGGTATAAAGGTGATCCAGACAAAGTGAAGAGGTGGAAGGATGCTCTTTTTGAAGTAGGCAATCTCTCTGGATGGCATCTGCAAGATGGGTATGAATCCAAAGTCATTCAAGAAATTGTTGAAAGGATTTTCACTGAGTTAAATCAATTAATCCCAATATCTGAGGGTTTAGTTGGGATGGATTCACACCTGAATGAAATGCTTTCATGCTTAGACATTGGGTGTCCTGATGTTCGTATCGTAGGGATTTGTGGTATGGGTGGTATCGGTAAGACAACTATTGCACAAGTGGTTTTTGAAAAGGTACAAGCTCAGTTTGAAGGTTGCAGCTTTCTCGAGAATGTTAGAGAGGTAATTGCAAAGCAAGGTGCAGTTCATTTACAAGAGCAACTTCTTTCAGACTTGATGAAATGTAATGTAAATGTACAGAACACTAAAATAGGAAAAAACATAATGAGGCAGAGACTACATGCTAAAATGGTTCTGATCATTCTTGATGACGTGGACCAAGAAGAACAATTGGAAGCACTGTGCGAGCGTGAATCGTTTGGTCCAGGGAGTAGAATCATTATAACTTCAAGAGATGAACATTTGCTCAGTGCAGTTGAAGGAGATAAAGTGTACAATGTGAATCCATTAACTGATGCTGAAGCTCTTCAGCTCTTTAGTATGAAAGCCTTTAAGAAAGACCAGCTGGTTGGAGAAGAAGTTCTCAAGCTATCCAAGGAATTTTTGAAATATGCTGATGGCCTTCCATTAGCTATTAAAGTTTTAGGTTCTTCTGTTAAGGGTAGAAGTGTAGAATTGTGGTCAAGTGCATTAGATAGACTAAAAAAAAGTCCTGATAAAAAAATTATAAATGTTCTCAAGGTTAGTTTTGATGGATTAGAACCAAGAGAACAGAAAATTTTTTTGGACATCGCATGTTTCCTTAAAGGGGTGAATATAGATCGTGTAAGAAGAATACTACAGGGTAGTGATGCCCACTGTCCAGACTTTGATATACAGGTTCTCCTGGACAAATCTATGGTAACTCTATTTGGAAAAAAAATGTGGATGCATGATTTGATACAAGAATTGGGTTGGGAAGTTGTTCGCCAAGAATGCCATGATGAACCTGGAAAACGTAGTAGATTGTGGCTTTCAAGTGAGATCATTGATGTACTTGATCAGAGTAAGGCAACGAGTGCAGTTCAAAGCATATTCCTCCAAGGTCCAACAAAAGATGATGTGGTTCACTCAGTTAACGAGTCTTTCTCAAATATGGACGGATTAAGATTGCTCAAAATTTGGAATGTGAAGTTTTCTGGAAACATCAAATATCTTTCTAAGAAGTTACAGTTTCTAGAATGGGATGAATGTCCTTTAGATTCCTTCCCTTCAGACTTTCAACCAGATTTCCTTGTCGAACTTCACATGCATTCAAGCCGCATCAAACAACTATGGATAGGACAAAGAGGATGGAGAATGCTACGGCTTATCGACATGAGTGGTGCACAATACTTGAAGTCAACTCCAGACTTCACTGAGGTTCCTGATCTTGAAACGTTGGTGCTTCAATATTGTACAAGCTTGTTGGAGGTTCACCCGTCTCTTGGATTTCTGAAGAAGCTTATTAATTTGAATATGACATGTTGCAAGTCTATTAAGAGCATTCCACCTTTCACTGCCTTGGAATCCCTTCAAATATTGAGGTTTTCATTCTGTTCAGGACTGAAGAAGTTTCCAGAAATTAAAGGAAATATGAAAAGCTTGCTGGAGCTTCATTTGGATGGGACCAACATTGAGGAATTGCCTCCATCAATTGAACGCTTGACTGGTCTTACCATGTTGAATCTAACAGACTGTAAAAACCTTTTGCATCTTCCCAATGACATTGGGTGCTTGACATCTTTGAAAAGTCTCTATCTTACAGGTTGCTCCAAAATTGATGAGATACCAGAGAATCTGAATGGTATGAAATGTCTGGAGAAGCTTGCAATTGGTGGGACTTCCATAAGGGAGTTATCTTTCATTCTAGGCATGAAGAATCTACAGTATCTATCCTGCCAAGGATGTAAATATCTAGTTCCAGAATCATGCAAAGGTCTGGCTTCATTGTCGAATTTGATACAGCTAGACTTAAGTTATTGCAATCTGATGGATGGAGCAATTCTCAATGATTTTAGCAGCCTATTCTACTTAACATTGTTAGATTTAAGTGGTAATTGCTTTGTGCGATTACCTGAAAGTATCTCTCAACTCTCAAAGCTCGAGACTCTGCGCTTGAGTAATTGCAGACAGCTTCAATTGCTGCCCAAGAAGCTTCCGTTAAGTCTTCAATACCTGTATGCAGAACAATGTACTTCACTGACAGATTACCCAAATCAAATCAAAGTATTGAATTCATCGGAGTCGGGAGTGACTATTGTGAATTCCCTCAATTCTTCAGCACCTGGAGACTTACAAAGTTCCACAATGTTTACTTTTCCAAGAACTGAGGGGGAATCTTTCCAACTGCATTTGACATATAAGCATACAGAGGGAAAACAAGTTGATCATTTGCCACTGAGAACTCTATTAACGATGCTTTTGTGTTCTACGTTGCAGATGCCTTTCGGGTCAAATTTCAGTAGCATATATCCTACAGATGAAATTCCAGAGTGGTTCAGCACTGTATCTACCAGAAATCCCATATCAATCTCGATACCTCAAAATCTGGCAGATGATGACAAGTGGAAGGGATTTGCCGCGTGTGCCATATTCTCAGTCAAGGGGCATACTGATCTCTCCCTTATTGAACCGGATCCTGATTTTCTTAATTACTCGTATCAAATCACATTTGAAACTGATGTTATGCGTCTGGAACCATATGTGTTGGAGTGGTATGAAGCACGCCGCCCCCTTGGATCAATTTCTCATCTGCTTATGATTTTCTATATATCTCGTTCGGAGGTTCCACAGAGGGGGTTTAATCAATCAACTGTGGTGAGGGCTATGTTTGAAACCACCAATGCATCCATGGTGGTCCAGAAATGTGGAATTCGTCTAGTCTATGAGCAAGATGCTGGATGGCATTACAGAATAGTCGGGGGCGATGATCCGATTTGTCTGAATAAAATTGAAGACGACGCCTCTCTATTGGAGTCTGGCTGGCTTAGTCAATATATGGAATGGGAGAAATTCATTCCACCCTTGGAAGAAGAATCCACACTTTTGCTGAGAAAGAACCTTCAGTCTGTTCTTCCAAGATACCTTGAGGCACTAAATCGTTATTCTGCAATGTACAAATTTAACCTCAAAGGAAGCCCAGGGTGGTTTCATAAGTCATTTGTCTCTAATACGAGGTTAGATTGGGCCAAAATGTCAATCGAGCTGCCTCAAAATCTACAAAAGAGCAAGAAGTGGATGGGATTTGCAATATACGCATCACTTGTACAGGAAGTGGGGCAGATAACGAAACAGAACAACTATCGGGTATATGTTACACTAAAAACGAGGGATAATTTACCTACATGGGTGGTTCGAGTACTTACCCTTAAGCACATCAGGCCATTGTCAGAAGAACATCATCAACTATTGGTTATTTACATACCACGGGCACAGATTCCTGAAGTGTTGTTCACTCAGACATTAACAACTGTAATGGACTTTTACATCAAGATCTGTGATAGCCCTCAAGTAAAGGCTCAAAGATGTGGGTTCCGCATTGTGTACCAAGAAGATATACAAGGGTTTGCTGAGACTGTTATCCGGTGCATGCAGAGGGAAGATTCTCTTAAATCTCATGATAAGTTGGTGGTTGAACAATGGATACAGTTGATACGATTGCAAGGTGCCCATATAGAGGGAATGAAAGAACGGGATTCCAAAACCCACAGGAAAACTACTTCGAATTGCGACTGGTCTGACCCTCGTCCTGTTTCTTGTCACTTCGAGGGAGCTGAAATTGTCAAGTCAAAATGGTTCATGCCTTTCATCAACCAAGGCAACTCTGCAGAAATCCAACTGCCTCTGAATGTGTTCGATGATGATAATTGGTTGGGATTTGCCGTATGTTCTCGCTTGTCCCACGACCAATTTACAAACATTAGTCCTGGCAGTACTGCTCCTGATCCAGAGGATGCTATACATACTTACCGTCTGGACTCTGATGTTGATTGGCACGGCTTATTTGATTTCACCATGCGTGCAGATATTAAGAAACATAAGCCATTGGAATACGATGCAACTCTGTTCCTTTATATACCGCGCACTAAGGTTCTTAGTAAGATGTGGAGACAATGCAAATTGGCCAGGATAACTATGTGGTTCTCTAGCCCATGCTTAGCGGTGCATAGTTGTGCCCTTCGTCTACTATTTAAGGAGGACGTTGAACATCTTGTAGAAACATTGACCCACGCCGAATTACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1665

Amino Acids

190.02

Weight (kDa)

6.39

Isoelectric Point (pI)

44.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 81 - 254 8.1e-51 TIR domain
TIR_2 PF13676 83 - 181 1.6e-16 TIR domain
NB-ARC PF00931 266 - 431 9.5e-24 NB-ARC domain
NACHT PF05729 281 - 428 9.3e-06 NACHT domain
WHD_ROQ1 PF23282 500 - 568 1.4e-14 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 759 - 846 9.9e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 762 - 839 1.2e-07 Leucine-rich repeat region
LRR_14 PF23598 826 - 904 3.2e-06 Leucine-rich repeat region
LRR_14 PF23598 907 - 997 9.1e-07 Leucine-rich repeat region
C-JID PF20160 1076 - 1217 6.3e-11 C-JID domain
C-JID PF20160 1516 - 1653 7.6e-06 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1175, 1460
AccB1I GGYRCC 1 cut(s) 4418
AccI GTMKAC 1 cut(s) 4936
AccII CGCG 2 cut(s) 3335, 4834
AclI AACGTT 1 cut(s) 2150
AclWI GGATC 8 cut(s) 7, 533, 611, 3383, 3396, 3499, 3677, 4691
AcoI YGGCCR 1 cut(s) 4875
AcuI CTGAAG 9 cut(s) 168, 1244, 1272, 1968, 2228, 2339, 3015, 3800, 4215
AcyI GRCGYC 1 cut(s) 3714
AfiI CCNNNNNNNGG 5 cut(s) 227, 1540, 2198, 3548, 4429
AflII CTTAAG 2 cut(s) 2725, 4121
AflIII ACRYGT 1 cut(s) 2234
AhlI ACTAGT 1 cut(s) 46
AjiI CACGTC 1 cut(s) 1105
AjnI CCWGG 7 cut(s) 1156, 1617, 1720, 3037, 3892, 4678, 4877
AjuI GAANNNNNNNTTGG 2 cut(s) 1499, 1531
AloI GAACNNNNNNTCC 2 cut(s) 1296, 1328
Alw21I GWGCWC 1 cut(s) 2101
Alw44I GTGCAC 1 cut(s) 2097
AlwI GGATC 8 cut(s) 7, 533, 611, 3383, 3396, 3499, 3677, 4691
AlwNI CAGNNNCTG 1 cut(s) 3038
Ama87I CYCGRG 2 cut(s) 938, 2855
AoxI GGCC 5 cut(s) 565, 1351, 3939, 4133, 4875
ApaLI GTGCAC 1 cut(s) 2097
ApeKI GCWGC 5 cut(s) 135, 930, 2772, 2896, 3958
AseI ATTAAT 2 cut(s) 752, 2220
Asp700I GAANNNNTTC 6 cut(s) 204, 1257, 1311, 1695, 2283, 4604
AspLEI GCGC 2 cut(s) 2868, 4836
AspS9I GGNCC 7 cut(s) 374, 1108, 1154, 1817, 2379, 3610, 3939
AsuHPI GGTGA 5 cut(s) 626, 1555, 2180, 3583, 4756
AsuII TTCGAA 1 cut(s) 4475
AvaI CYCGRG 2 cut(s) 938, 2855
AvaII GGWCC 6 cut(s) 374, 1108, 1154, 1817, 2379, 3610
BaeGI GKGCMC 4 cut(s) 2101, 4186, 4423, 4930
BalI TGGCCA 1 cut(s) 4877
BamHI GGATCC 1 cut(s) 3388
BanI GGYRCC 1 cut(s) 4418
BbsI GAAGAC 2 cut(s) 2912, 3714
Bbv12I GWGCWC 1 cut(s) 2101
BbvI GCAGC 5 cut(s) 122, 942, 2784, 2883, 3945
BceAI ACGGC 3 cut(s) 2093, 4619, 4765
BcgI CGANNNNNNTGC 2 cut(s) 919, 953
BciT130I CCWGG 7 cut(s) 1158, 1619, 1722, 3039, 3894, 4680, 4879
BciVI GTATCC 1 cut(s) 4389
BclI TGATCA 3 cut(s) 1089, 1771, 3139
BcuI ACTAGT 1 cut(s) 46
BfaI CTAG 7 cut(s) 47, 1949, 2621, 2669, 2720, 3632, 4899
BfmI CTRYAG 4 cut(s) 1571, 2636, 3228, 4578
BfrI CTTAAG 2 cut(s) 2725, 4121
BfuI GTATCC 1 cut(s) 4389
BglII AGATCT 1 cut(s) 4245
BisI GCNGC 9 cut(s) 133, 136, 931, 2032, 2773, 2897, 3333, 3481, 3959
BlpI GCTNAGC 1 cut(s) 4909
BlsI GCNGC 9 cut(s) 134, 137, 932, 2033, 2774, 2898, 3334, 3482, 3960
BmcAI AGTACT 2 cut(s) 4113, 4687
Bme1390I CCNGG 7 cut(s) 1158, 1619, 1722, 3039, 3894, 4680, 4879
Bme18I GGWCC 6 cut(s) 374, 1108, 1154, 1817, 2379, 3610
BmeT110I CYCGRG 2 cut(s) 938, 2855
BmgBI CACGTC 1 cut(s) 1105
BmgT120I GGNCC 7 cut(s) 374, 1108, 1154, 1817, 2379, 3610, 3939
BmiI GGNNCC 7 cut(s) 2136, 2380, 3390, 3450, 3540, 4286, 4420
BmrFI CCNGG 7 cut(s) 1158, 1619, 1722, 3039, 3894, 4680, 4879
BmrI ACTGGG 2 cut(s) 168, 515
BmuI ACTGGG 2 cut(s) 168, 515
BoxI GACNNNNGTC 1 cut(s) 4497
BpiI GAAGAC 2 cut(s) 2912, 3714
BplI GAGNNNNNCTC 4 cut(s) 1844, 1876, 2742, 2774
BpmI CTGGAG 4 cut(s) 2103, 2381, 2591, 3060
Bpu1102I GCTNAGC 1 cut(s) 4909
Bpu14I TTCGAA 1 cut(s) 4475
BpuEI CTTGAG 5 cut(s) 1301, 1454, 2890, 3860, 4245
Bsa29I ATCGAT 1 cut(s) 368
BsaBI GATNNNNATC 1 cut(s) 537
BsaHI GRCGYC 1 cut(s) 3714
BsaI GGTCTC 1 cut(s) 151
BsaWI WCCGGW 3 cut(s) 227, 3385, 4335
BsaXI ACNNNNNCTCC 6 cut(s) 1206, 1236, 1296, 1326, 1599, 1629
Bsc4I CCNNNNNNNGG 5 cut(s) 227, 1540, 2198, 3548, 4429
Bse1I ACTGG 4 cut(s) 174, 521, 2425, 4490
Bse3DI GCAATG 1 cut(s) 3870
Bse8I GATNNNNATC 1 cut(s) 537
BseBI CCWGG 7 cut(s) 1158, 1619, 1722, 3039, 3894, 4680, 4879
BseCI ATCGAT 1 cut(s) 368
BseJI GATNNNNATC 1 cut(s) 537
BseLI CCNNNNNNNGG 5 cut(s) 227, 1540, 2198, 3548, 4429
BseMI GCAATG 1 cut(s) 3870
BseNI ACTGG 4 cut(s) 174, 521, 2425, 4490
BseRI GAGGAG 1 cut(s) 210
BseSI GKGCMC 4 cut(s) 2101, 4186, 4423, 4930
BseXI GCAGC 5 cut(s) 122, 942, 2784, 2883, 3945
BsgI GTGCAG 4 cut(s) 993, 1224, 1812, 4794
Bsh1236I CGCG 2 cut(s) 3335, 4834
BshFI GGCC 5 cut(s) 567, 1353, 3941, 4135, 4877
BshNI GGYRCC 1 cut(s) 4418
BshVI ATCGAT 1 cut(s) 368
BsiHKAI GWGCWC 1 cut(s) 2101
BsiHKCI CYCGRG 2 cut(s) 938, 2855
BsiSI CCGG 3 cut(s) 228, 3386, 4336
BsiWI CGTACG 1 cut(s) 240
BslFI GGGAC 3 cut(s) 2392, 2605, 4636
BslI CCNNNNNNNGG 5 cut(s) 227, 1540, 2198, 3548, 4429
BsmBI CGTCTC 1 cut(s) 2199
BsmFI GGGAC 3 cut(s) 2392, 2605, 4636
BsmI GAATGC 5 cut(s) 488, 1712, 2023, 2076, 2256
BsnI GGCC 5 cut(s) 567, 1353, 3941, 4135, 4877
Bso31I GGTCTC 1 cut(s) 151
BsoBI CYCGRG 2 cut(s) 938, 2855
Bsp119I TTCGAA 1 cut(s) 4475
Bsp1286I GDGCHC 4 cut(s) 2101, 4186, 4423, 4930
Bsp1407I TGTACA 5 cut(s) 1027, 1224, 2168, 3867, 4018
Bsp1720I GCTNAGC 1 cut(s) 4909
Bsp19I CCATGG 1 cut(s) 3603
BspANI GGCC 5 cut(s) 567, 1353, 3941, 4135, 4877
BspDI ATCGAT 1 cut(s) 368
BspFNI CGCG 2 cut(s) 3335, 4834
BspHI TCATGA 1 cut(s) 4369
BspLI GGNNCC 7 cut(s) 2136, 2380, 3390, 3450, 3540, 4286, 4420
BspMAI CTGCAG 1 cut(s) 4582
BspPI GGATC 8 cut(s) 7, 533, 611, 3383, 3396, 3499, 3677, 4691
BspQI GCTCTTC 1 cut(s) 1263
BspT104I TTCGAA 1 cut(s) 4475
BspT107I GGYRCC 1 cut(s) 4418
BspTI CTTAAG 2 cut(s) 2725, 4121
BspTNI GGTCTC 1 cut(s) 151
BsrDI GCAATG 1 cut(s) 3870
BsrGI TGTACA 5 cut(s) 1027, 1224, 2168, 3867, 4018
BsrI ACTGG 4 cut(s) 174, 521, 2425, 4490
BssNI GRCGYC 1 cut(s) 3714
BssT1I CCWWGG 8 cut(s) 1326, 1813, 2274, 2653, 3487, 3603, 3777, 4567
Bst2UI CCWGG 7 cut(s) 1158, 1619, 1722, 3039, 3894, 4680, 4879
Bst6I CTCTTC 3 cut(s) 393, 626, 1263
BstACI GRCGYC 1 cut(s) 3714
BstAFI CTTAAG 2 cut(s) 2725, 4121
BstAPI GCANNNNNTGC 1 cut(s) 1790
BstAUI TGTACA 5 cut(s) 1027, 1224, 2168, 3867, 4018
BstBI TTCGAA 1 cut(s) 4475
BstC8I GCNNGC 7 cut(s) 1139, 2357, 2580, 3478, 3737, 4343, 4773
BstDSI CCRYGG 2 cut(s) 3603, 4178
BstFNI CGCG 2 cut(s) 3335, 4834
BstHHI GCGC 2 cut(s) 2868, 4836
BstMWI GCNNNNNNNGC 9 cut(s) 446, 476, 1707, 1790, 2893, 2905, 3338, 4007, 4132
BstNI CCWGG 7 cut(s) 1158, 1619, 1722, 3039, 3894, 4680, 4879
BstNSI RCATGY 5 cut(s) 1076, 1530, 2023, 2238, 4345
BstPAI GACNNNNGTC 1 cut(s) 4497
BstSCI CCNGG 7 cut(s) 1156, 1617, 1720, 3037, 3892, 4678, 4877
BstSFI CTRYAG 4 cut(s) 1571, 2636, 3228, 4578
BstSLI GKGCMC 4 cut(s) 2101, 4186, 4423, 4930
BstUI CGCG 2 cut(s) 3335, 4834
BstV1I GCAGC 5 cut(s) 122, 942, 2784, 2883, 3945
BstV2I GAAGAC 2 cut(s) 2912, 3714
BstX2I RGATCY 2 cut(s) 3388, 4245
BstXI CCANNNNNNTGG 2 cut(s) 1301, 3620
BstYI RGATCY 2 cut(s) 3388, 4245
Bsu15I ATCGAT 1 cut(s) 368
BsuI GTATCC 1 cut(s) 4389
BsuRI GGCC 5 cut(s) 567, 1353, 3941, 4135, 4877
BsuTUI ATCGAT 1 cut(s) 368
BtgI CCRYGG 2 cut(s) 3603, 4178
BtgZI GCGATG 2 cut(s) 1507, 3693
BtrI CACGTC 1 cut(s) 1105
BtsI GCAGTG 2 cut(s) 303, 2268
Cac8I GCNNGC 7 cut(s) 1139, 2357, 2580, 3478, 3737, 4343, 4773
CaiI CAGNNNCTG 1 cut(s) 3038
CciI TCATGA 1 cut(s) 4369
CfoI GCGC 2 cut(s) 2868, 4836
Cfr13I GGNCC 7 cut(s) 374, 1108, 1154, 1817, 2379, 3610, 3939
ClaI ATCGAT 1 cut(s) 368
CseI GACGC 2 cut(s) 3431, 3722
EaeI YGGCCR 1 cut(s) 4875
Eam1104I CTCTTC 3 cut(s) 393, 626, 1263
EarI CTCTTC 3 cut(s) 393, 626, 1263
EciI GGCGGA 1 cut(s) 5
Eco130I CCWWGG 8 cut(s) 1326, 1813, 2274, 2653, 3487, 3603, 3777, 4567
Eco31I GGTCTC 1 cut(s) 151
Eco47I GGWCC 6 cut(s) 374, 1108, 1154, 1817, 2379, 3610
Eco57I CTGAAG 9 cut(s) 168, 1244, 1272, 1968, 2228, 2339, 3015, 3800, 4215
Eco88I CYCGRG 2 cut(s) 938, 2855
EcoRI GAATTC 3 cut(s) 2986, 3016, 3624
EcoRII CCWGG 7 cut(s) 1156, 1617, 1720, 3037, 3892, 4678, 4877
EcoT14I CCWWGG 8 cut(s) 1326, 1813, 2274, 2653, 3487, 3603, 3777, 4567
EcoT22I ATGCAT 4 cut(s) 488, 1665, 2025, 3601
ErhI CCWWGG 8 cut(s) 1326, 1813, 2274, 2653, 3487, 3603, 3777, 4567
Esp3I CGTCTC 1 cut(s) 2199
FalI AAGNNNNNCTT 4 cut(s) 978, 1010, 4344, 4376
FaqI GGGAC 3 cut(s) 2392, 2605, 4636
FauI CCCGC 1 cut(s) 284
FauNDI CATATG 2 cut(s) 544, 3454
FbaI TGATCA 3 cut(s) 1089, 1771, 3139
FblI GTMKAC 1 cut(s) 4936
Fnu4HI GCNGC 9 cut(s) 133, 136, 931, 2032, 2773, 2897, 3333, 3481, 3959
Fsp4HI GCNGC 9 cut(s) 133, 136, 931, 2032, 2773, 2897, 3333, 3481, 3959
FspBI CTAG 7 cut(s) 47, 1949, 2621, 2669, 2720, 3632, 4899
GlaI GCGC 2 cut(s) 2867, 4835
GluI GCNGC 9 cut(s) 133, 136, 931, 2032, 2773, 2897, 3333, 3481, 3959
GsuI CTGGAG 4 cut(s) 2103, 2381, 2591, 3060
HaeIII GGCC 5 cut(s) 567, 1353, 3941, 4135, 4877
HapII CCGG 3 cut(s) 228, 3386, 4336
HgaI GACGC 2 cut(s) 3431, 3722
HhaI GCGC 2 cut(s) 2868, 4836
Hin1I GRCGYC 1 cut(s) 3714
Hin6I GCGC 2 cut(s) 2866, 4834
HinP1I GCGC 2 cut(s) 2866, 4834
HincII GTYRAC 2 cut(s) 1849, 2115
HindII GTYRAC 2 cut(s) 1849, 2115
HindIII AAGCTT 5 cut(s) 2173, 2213, 2353, 2576, 2906
HpaI GTTAAC 1 cut(s) 1849
HpaII CCGG 3 cut(s) 228, 3386, 4336
HphI GGTGA 5 cut(s) 626, 1555, 2180, 3583, 4756
Hpy99I CGWCG 1 cut(s) 3716
HpyCH4IV ACGT 6 cut(s) 239, 1104, 1729, 2150, 3185, 4953
HpyF10VI GCNNNNNNNGC 9 cut(s) 446, 476, 1707, 1790, 2893, 2905, 3338, 4007, 4132
HpySE526I ACGT 6 cut(s) 239, 1104, 1729, 2150, 3185, 4953
Hsp92I GRCGYC 1 cut(s) 3714
HspAI GCGC 2 cut(s) 2866, 4834
Ksp22I TGATCA 3 cut(s) 1089, 1771, 3139
KspAI GTTAAC 1 cut(s) 1849
LguI GCTCTTC 1 cut(s) 1263
LmnI GCTCC 5 cut(s) 2362, 2528, 2750, 4523, 4696
Lsp1109I GCAGC 5 cut(s) 122, 942, 2784, 2883, 3945
MaeI CTAG 7 cut(s) 47, 1949, 2621, 2669, 2720, 3632, 4899
MaeII ACGT 6 cut(s) 239, 1104, 1729, 2150, 3185, 4953
MaeIII GTNAC 7 cut(s) 187, 434, 1633, 1938, 3004, 4066, 4511
MfeI CAATTG 4 cut(s) 1121, 2406, 2583, 2891
MflI RGATCY 2 cut(s) 3388, 4245
MhlI GDGCHC 4 cut(s) 2101, 4186, 4423, 4930
MlsI TGGCCA 1 cut(s) 4877
MluNI TGGCCA 1 cut(s) 4877
MlyI GAGTC 6 cut(s) 410, 1862, 2854, 3005, 3737, 4724
MmeI TCCRAC 6 cut(s) 1282, 1844, 2160, 3121, 3441, 4612
Mox20I TGGCCA 1 cut(s) 4877
Mph1103I ATGCAT 4 cut(s) 488, 1665, 2025, 3601
MroXI GAANNNNTTC 6 cut(s) 204, 1257, 1311, 1695, 2283, 4604
MscI TGGCCA 1 cut(s) 4877
MslI CAYNNNNRTG 3 cut(s) 795, 2373, 4923
Msp20I TGGCCA 1 cut(s) 4877
MspA1I CMGCKG 2 cut(s) 264, 1297
MspCI CTTAAG 2 cut(s) 2725, 4121
MspI CCGG 3 cut(s) 228, 3386, 4336
MspR9I CCNGG 7 cut(s) 1158, 1619, 1722, 3039, 3894, 4680, 4879
MunI CAATTG 4 cut(s) 1121, 2406, 2583, 2891
Mva1269I GAATGC 5 cut(s) 488, 1712, 2023, 2076, 2256
MvaI CCWGG 7 cut(s) 1158, 1619, 1722, 3039, 3894, 4680, 4879
MvnI CGCG 2 cut(s) 3335, 4834
MwoI GCNNNNNNNGC 9 cut(s) 446, 476, 1707, 1790, 2893, 2905, 3338, 4007, 4132
NcoI CCATGG 1 cut(s) 3603
NdeI CATATG 2 cut(s) 544, 3454
NlaIV GGNNCC 7 cut(s) 2136, 2380, 3390, 3450, 3540, 4286, 4420
NmuCI GTSAC 3 cut(s) 187, 3004, 4511
NsiI ATGCAT 4 cut(s) 488, 1665, 2025, 3601
NspI RCATGY 5 cut(s) 1076, 1530, 2023, 2238, 4345
NspV TTCGAA 1 cut(s) 4475
PaeI GCATGC 1 cut(s) 4345
PaeR7I CTCGAG 2 cut(s) 938, 2855
PagI TCATGA 1 cut(s) 4369
PasI CCCWGGG 1 cut(s) 3893
PciI ACATGT 1 cut(s) 2234
PciSI GCTCTTC 1 cut(s) 1263
PctI GAATGC 5 cut(s) 488, 1712, 2023, 2076, 2256
PdmI GAANNNNTTC 6 cut(s) 204, 1257, 1311, 1695, 2283, 4604
Pfl23II CGTACG 1 cut(s) 240
PfoI TCCNGGA 1 cut(s) 1617
PkrI GCNGC 9 cut(s) 134, 137, 932, 2033, 2774, 2898, 3334, 3482, 3960
PleI GAGTC 6 cut(s) 409, 1861, 2854, 3004, 3736, 4724
PpsI GAGTC 6 cut(s) 409, 1861, 2854, 3004, 3736, 4724
PscI ACATGT 1 cut(s) 2234
PshAI GACNNNNGTC 1 cut(s) 4497
PshBI ATTAAT 2 cut(s) 752, 2220
PsiI TTATAA 2 cut(s) 1175, 1460
Psp1406I AACGTT 1 cut(s) 2150
Psp6I CCWGG 7 cut(s) 1156, 1617, 1720, 3037, 3892, 4678, 4877
PspGI CCWGG 7 cut(s) 1156, 1617, 1720, 3037, 3892, 4678, 4877
PspLI CGTACG 1 cut(s) 240
PspN4I GGNNCC 7 cut(s) 2136, 2380, 3390, 3450, 3540, 4286, 4420
PspPI GGNCC 7 cut(s) 374, 1108, 1154, 1817, 2379, 3610, 3939
PsrI GAACNNNNNNTAC 6 cut(s) 89, 121, 233, 265, 4380, 4412
PstI CTGCAG 1 cut(s) 4582
PstNI CAGNNNCTG 1 cut(s) 3038
PsuI RGATCY 2 cut(s) 3388, 4245
PvuII CAGCTG 1 cut(s) 1297
RseI CAYNNNNRTG 3 cut(s) 795, 2373, 4923
SapI GCTCTTC 1 cut(s) 1263
SatI GCNGC 9 cut(s) 133, 136, 931, 2032, 2773, 2897, 3333, 3481, 3959
Sau96I GGNCC 7 cut(s) 374, 1108, 1154, 1817, 2379, 3610, 3939
ScaI AGTACT 2 cut(s) 4113, 4687
SchI GAGTC 6 cut(s) 410, 1862, 2854, 3005, 3737, 4724
ScrFI CCNGG 7 cut(s) 1158, 1619, 1722, 3039, 3894, 4680, 4879
SduI GDGCHC 4 cut(s) 2101, 4186, 4423, 4930
SfcI CTRYAG 4 cut(s) 1571, 2636, 3228, 4578
Sfr274I CTCGAG 2 cut(s) 938, 2855
SfuI TTCGAA 1 cut(s) 4475
SinI GGWCC 6 cut(s) 374, 1108, 1154, 1817, 2379, 3610
SlaI CTCGAG 2 cut(s) 938, 2855
SmiMI CAYNNNNRTG 3 cut(s) 795, 2373, 4923
SmlI CTYRAG 9 cut(s) 938, 1316, 1469, 2725, 2855, 2869, 3839, 4121, 4260
SmoI CTYRAG 9 cut(s) 938, 1316, 1469, 2725, 2855, 2869, 3839, 4121, 4260
SpeI ACTAGT 1 cut(s) 46
SphI GCATGC 1 cut(s) 4345
SspI AATATT 2 cut(s) 2165, 2292
SspMI CTAG 7 cut(s) 47, 1949, 2621, 2669, 2720, 3632, 4899
StyD4I CCNGG 7 cut(s) 1156, 1617, 1720, 3037, 3892, 4678, 4877
StyI CCWWGG 8 cut(s) 1326, 1813, 2274, 2653, 3487, 3603, 3777, 4567
TaiI ACGT 6 cut(s) 242, 1107, 1732, 2153, 3188, 4956
TauI GCSGC 4 cut(s) 135, 2034, 3335, 3483
TseFI GTSAC 3 cut(s) 187, 3004, 4511
TseI GCWGC 5 cut(s) 135, 930, 2772, 2896, 3958
Tsp45I GTSAC 3 cut(s) 187, 3004, 4511
TspGWI ACGGA 4 cut(s) 508, 568, 1887, 2901
Vha464I CTTAAG 2 cut(s) 2725, 4121
VneI GTGCAC 1 cut(s) 2097
VpaK11BI GGWCC 6 cut(s) 374, 1108, 1154, 1817, 2379, 3610
VspI ATTAAT 2 cut(s) 752, 2220
XbaI TCTAGA 1 cut(s) 1948
XceI RCATGY 5 cut(s) 1076, 1530, 2023, 2238, 4345
XcmI CCANNNNNNNNNTGG 2 cut(s) 1718, 3601
XhoI CTCGAG 2 cut(s) 938, 2855
XmiI GTMKAC 1 cut(s) 4936
XmnI GAANNNNTTC 6 cut(s) 204, 1257, 1311, 1695, 2283, 4604
XspI CTAG 7 cut(s) 47, 1949, 2621, 2669, 2720, 3632, 4899
ZrmI AGTACT 2 cut(s) 4113, 4687
Zsp2I ATGCAT 4 cut(s) 488, 1665, 2025, 3601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.