Rw7G041410

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000129
Physical Location & Seq
Forward (+)
64600338 .. 64613064
12727 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G041410.1

Sequence Viewer

Length: 4863 bp
ATGACTACCAAAACAGATCTTCCTTCTTCTTCTTCTCCTCCTTCTCCTCCTCCATTTCGTAAATTGACATACGATGTGTTCCTCAGTTTCAGAGGTCAAGATACCCGTACAAATTTCACAGACCATCTTTATACCGCTCTCAAACAGAAAGGAATAAACACATTTAGAGATGATGAAGAACTTAACAAAGGGGAATCAATTGGTCCTAATCTCTTGAAAGCAATTGAAGAATCAAGATATGTAATTGCCGTTTTCTCACGAAATTACGCCAATTCTGCATGGTGTTTGGATGAGATTGCAAAGGTTGCTGAATGCATGAAAGAAATGGGACAAAAAGTCTTTCCAGTGTTCTACGATGTTGATCCATCTGAGGTACGAAGACAAACAGGGGAGCACTTTGGGAAAGCATGTGAGAAGCATCAAAAGCGGTTTAAAGCAGAACCAGATAAAGTAAAGAGGTGGAAAGACGCTCTTGCTGAAGTAAGCAATCTTTCTGGATGGCATCTGCAAAATGGGTCTGAAGCCAAAATTATTCAAGAAATTGTAGAAAAGATTTTCACTGAGTTGAATAAAATAATCTCCACATGTGAGGGCTTAGTTGGGATGGATTCTCACCTGAATGAACTGTTTTCCTACTTAGACATTGGGTGTCTTGATGTTCGTATAATAGGGATTTGTGGGATGGGTGGTATCGGAAAAACTACTATTGCACAAGTGCTTTTTGAAAGGGTAAAAGCTCAGTTTGCAGGTGATAGCTTTCTTGAGAATGTTAAAGAAGAAACTGAAAATAAGCAAGGTAGTACAATTCATTTACAAGAGAAACTTCTATTGAACTTGCTGAATAGTAATGTCAATGTACAGAACACTAAAACGGGAAAAGACATAATTAAGCATAGACTATCTGCTAAAAGAGTGCTTGTTGTTCTTGATGATGTGGATCAAGATAAACAATTGGAAACATTGTGTGATCGTACTTGGTTTGGTCCAGGGAGTAGAATCATCATAACATCCAGGGATGAACACTTGATCAGTGCATTTGGAGCAGATAAAGTATACAAGGTGAATGCATTAACTGATCCTGAAGCTTTTGAGCTCTTTAGTTTGAAAGCCTTTAAGGAAGACAAGGTTGGAGAAGATTTTCTCAAGCTATCCAAGGAATTTTTGAAATATGCCAATGGCCTTCCATTAGCTATTGAAGTTTTGGGATCGTCTGTTCGTGGTAGAAGTGTAAAGTTATGGTCAAGTGCATTGAAGAGACTTAAAAAGAATCCTCAAAAAGGGATTATTGATGTTCTTAAAGTTAGTTTTGATGAATTAGAAGAAAGAGAAAAGAAAACGTTTTTGGACATCGCGTGTTTCTTTAAAGGAGTGAACAAGGATCGTGTAATAAGAATACTAAATGGTTGTGACGATGACGGTGTGGACCTGGATGTACAAGTTCTTATGGACAAATCTTTGGTAACTCTATTTGGAACAAAATTGTGGATGCATGATTTGATACAAGAAATGGGTTGGGAAGTTGTTCGTCAAGAATGCCGTGAAGAACCTGGAAAACGTAGCAGGTTGTGGCTTCTCGATGAAATCATCCATGTACTTGGTCGACGTAAGGCGACGAGTGCAGTTCAAAGCATATTCCTCTCATGTCTAACAAAAGACGATGCTGTTCACTCAATTGATAATGCTTTCTCAGATATGGACAGACTGAGATTGCTCAAGATTTGGAATGTGAAGTTTTCCGGAAACATCAGATATCTTTCTAATGAGTTACAGTATCTTGATTGGCATGAGTGCCCTTTGGATTCTTTCCCATCAGATTTCCAACCAGATAAACTTGTTGAACTTTACATGTGTATTAGCAGCATCAAACAACTATGGAGGGGAAAAAAAGGATGGAGCATGCTACGGCTTATTAATCTGTGGGGTTCACAATACTTGATGTCAACTCCAGACTTCACTGAGGTTCCTGATCTTGAAGTGTTAGTGCTTCAAAATTGCACAAGCTTGGTGGAGGTTCACCCGTCTCTTGGATTTCTGAAGAAACTTCGCTATTTGGATATGATAAATTGCAAGTCTATTGAGAGCCTTCCACCTTTCACTAGCTTGGAATCCCTTGAAAAATTATGGCTTTCACTCTGTTCAGGACTGAAGAAGTTTCCAGAAATTGAAGGAAATATGAAAAGCTTGCTGGAGCTTCATTTGGATGGGACCAGCATTGAGGAATTGCCTCCATCGATTGAACGCTTGACTGGTCTAACCATGTTGAATCTGAAAGATTGTAAAAACCTTTTGCATCTTCCCAATACCATTGGATGCTTGACATCTTTGAAAAGTCTCTATCTAACAGGTTGCTCCAAAATTGATCAGATACCTGAGAATCTGAATGGTATAAAATGTCTGGAGAAGCTTTGCATTGGTGGAACTTCCATAAGGGAGTTATCTTTCATTGTAGGCATGAAGAATCTACAGTATCTATCCTGCCGAGGATGTAGATGTATAGTTTCAGAATCATGCAAAGGTCTGGCTTCGTTATCGCATTTGATAGAGCTAGACTTGAGTTATTGCAATCTGATGGATGGAGCAATTCTCAATGATTTTAGCAGCCTAATCTCCTTAAGAAGGCTAGATTTAAGTGGTAATTGCTTTGTGCGATTACCTGAAAGTATCTCTCAACTCTCAAAGCTCGAGGCTCTGTACTTGAATAATTGTAGACAGCTTCAATTGGTGCCCAAGAAGCTTCCGTTTAGTCTTCAAAACGTGTATGCAGAAGAATGTACTTCACTGACGGATTACCCAAATCAAATCATAGTATTGGTTTCACCGGAGTCAGGAGTGACTACTGTCGATTCCCTCAATTCTTCAGCACTTGGAGTCCCACAAAGTTCCAGAATGTCGGCTTTTTCAACAACTGAGGGGGGATCTTTAGAGCTGCATGTGGCATATAAGCATACAGAGGGGAAACATGTTGATCTTTGTCCAGCCGCAAATCGAGCGTTTGTAGAACAAACAACTTCCTGGTCAAAATTCGCTTCTGTACTTCCTAGAGATGAAATTCCAGAGTGGTTCAGCACTATATCTACCAGAAATCCCATATCAATCTCGATACCTCAAAATCTGGCCGATGATAACGAGTGGAGGGGATTTGCCGCGTGTGTTATATTCTCAGCCAAGGGGCATACTGATATCTCCCTTATTGAACCGGATCCTGATTTTCTTAATTACTCGTATCAAATCACATTGGAAACTGATGTTGTGCAACTGGAACCATTTGTGTTCGAGGGGTATGAAGCACTCCACCGCTTTGGATCAAGTTCTCATATGCTTATGATTTTCTATAAATCAAGTGGGGAGATTCCACAGTGGGGGTTAAATGAATCAACTGTGGTGAGAGCTATATTTGAAACCACCAATCCATCCATGGTGGTCCAGAAATGTGGAATCCGTCTAGTCTATGAGCAAGATGCTGGATGGTATCCCAGAATAATCTACGGGCATGGTCCGATTTGGCTGAATGATGTTGAGGACGACGCCTCTGATGTTGAGGACGATGTCTCTGATGTTGAGGACGACGCCTCCGTATTGGAGTCTGGCTGGCTTGGTCAGTCTTTGGAATGGGAGAAATTCATTCCACCCTTGGAAGAAGAATCCACACTCGTGCGGAGAAAGAACCTCGAGTCTGTTCTTCCAAGATACCTTGAGGCACTACACAGTGATTCTAAAATGTTCAAATTTAACCTCAAAGAGAGCCCAGGGTGGTTTGAGTTGTTTGTCCCTAATATGAGGTCAAATTTGATCGAAGAGTCAATCGAGCTGCCTCAAAATCTACACAAGAGCAAGAAGTGGATGGGATTTGCAATATGCGCATCACTTGTAGAGGAAGTGGGGCAGCAGACAATGAAAGAGGACAACTATTGGGTATCTGTTACACTATCAACAGGGGATAAATTTGGTATGGGTGTGTCCCGAGTACTTAAGCACGTCAGGCCATTGTCAGAAGAACATCATCAACTATTGGTTATTTACATACCGCGAGCACAGATTCCCGAAGTGTTGCTCACTCAGACATTAATAACTGCAATGGACTTTTGTATCATGATCAGTGATAGCCCTCATGTAAAGGTTCAAACATGTGGGTCACGTATTGTGTACCAGGAAGATATATTAGGGTTTGCCAATACAATTATCCGGTGTATCCAGAGGGAAGATTCTCTTGAATTTCATGATAAGTTAGTGATAGAAGAATGGATAAAGTTGATACGATTGCAAGGTCTCCATATAGAGGGAATGAAAGAACGGGATTCCAGAACCCCACGGGAAAAATACATTGATTTGTACTCACGAAAATATAGAAATTGGGAATGGTCTGACCCTCGTCCTGTTTGTTGTGCCTTCGAGAGCGTTGAAATCTCCAAGCTGAAATGGTTCATGCCTTTCATCAACCAAGGCAACTCTGTAGAAATTCAACTGCCTCTGAATGTGTTCGATGATGATAATTGGTTGGGGTTTGCCGTATGTTCTCAGTTGTCCCACGAGCAATATCCACACATTAGTCTTGGCAATAGTACTCTTGATTCAGAGAGAGTTGTGCTTAGTTATCGTCTGGACTCTGATGTTGCTTGGCACGGCTTATTTGATTTCCTGATGCATGAAGATATTAATGTACTCGAGCCATCCTATGCGACTCGGTTCCTTTATATACCGCGCACTGAAGTTCTTAGTAAGATGTGGAGGCAATGCAAATTGGCTAGGATAACTATGTGGTTCTCTAGCCCATGCTTAGCAGTGCATAGTTGTGCCCTTCGTCTACTATTTAAGGAGGACATTGAACATCTTGTAGAAACACTGACCCACGCCGAATTACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1620

Amino Acids

184.51

Weight (kDa)

5.46

Isoelectric Point (pI)

44.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 24 - 195 5.5e-55 TIR domain
TIR_2 PF13676 26 - 122 3.3e-17 TIR domain
NB-ARC PF00931 207 - 374 4.7e-22 NB-ARC domain
WHD_ROQ1 PF23282 440 - 510 1.1e-14 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 669 - 781 3e-09 Leucine-rich repeat region
LRR_13 PF23286 701 - 787 3.2e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 840 - 910 1.6e-06 Leucine-rich repeat region
C-JID PF20160 1012 - 1154 9.6e-10 C-JID domain
C-JID PF20160 1473 - 1608 5.4e-06 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 737
Acc16I TGCGCA 1 cut(s) 3859
Acc36I ACCTGC 2 cut(s) 737, 1551
AccB1I GGYRCC 1 cut(s) 2722
AccBSI CCGCTC 1 cut(s) 137
AccI GTMKAC 4 cut(s) 1053, 1600, 2707, 4801
AccII CGCG 4 cut(s) 1352, 3146, 4027, 4699
AccIII TCCGGA 1 cut(s) 1736
AciI CCGC 8 cut(s) 135, 427, 2979, 3144, 3295, 3655, 4025, 4697
AclI AACGTT 1 cut(s) 1337
AclWI GGATC 9 cut(s) 356, 945, 1070, 1213, 1386, 2923, 3194, 3207, 3310
AcoI YGGCCR 1 cut(s) 3114
AcuI CTGAAG 7 cut(s) 498, 540, 1101, 2054, 2165, 2841, 4725
AcyI GRCGYC 2 cut(s) 3525, 3567
AfiI CCNNNNNNNGG 6 cut(s) 1277, 2024, 2616, 2825, 3359, 4276
AflII CTTAAG 2 cut(s) 2611, 3968
AflIII ACRYGT 5 cut(s) 584, 1845, 2754, 2959, 4124
AhdI GACNNNNNGTC 1 cut(s) 335
AjiI CACGTC 1 cut(s) 3976
AjnI CCWGG 7 cut(s) 985, 1010, 1425, 1546, 3011, 3745, 4146
AjuI GAANNNNNNNTTGG 4 cut(s) 1110, 1142, 1325, 1357
AleI CACNNNNGTG 1 cut(s) 3650
AloI GAACNNNNNNTCC 2 cut(s) 1197, 1229
Alw21I GWGCWC 3 cut(s) 396, 1095, 4033
Alw26I GTCTC 5 cut(s) 1249, 2025, 2336, 3553, 4271
AlwI GGATC 9 cut(s) 356, 945, 1070, 1213, 1386, 2923, 3194, 3207, 3310
Ama87I CYCGRG 4 cut(s) 2681, 3668, 3960, 4661
Aor13HI TCCGGA 1 cut(s) 1736
AoxI GGCC 3 cut(s) 1177, 3114, 3980
ApeKI GCWGC 5 cut(s) 1857, 2598, 2926, 3808, 3883
AseI ATTAAT 3 cut(s) 1911, 4064, 4653
Asp700I GAANNNNTTC 4 cut(s) 1137, 1521, 4418, 4475
AspLEI GCGC 2 cut(s) 3860, 4701
AspS9I GGNCC 6 cut(s) 203, 983, 1423, 2205, 3421, 3494
AsuHPI GGTGA 6 cut(s) 605, 761, 1072, 2006, 2808, 3394
AvaI CYCGRG 4 cut(s) 2681, 3668, 3960, 4661
AvaII GGWCC 6 cut(s) 203, 983, 1423, 2205, 3421, 3494
BaeGI GKGCMC 3 cut(s) 1793, 2727, 4795
BaeI ACNNNNGTAYC 2 cut(s) 4069, 4102
BamHI GGATCC 1 cut(s) 3199
BanI GGYRCC 1 cut(s) 2722
BanII GRGCYC 2 cut(s) 1095, 3746
BauI CACGAG 2 cut(s) 3650, 4526
BbsI GAAGAC 3 cut(s) 385, 1125, 2738
Bbv12I GWGCWC 3 cut(s) 396, 1095, 4033
BbvI GCAGC 5 cut(s) 1869, 2610, 2913, 3795, 3895
BceAI ACGGC 5 cut(s) 233, 1521, 1919, 4490, 4636
BciT130I CCWGG 7 cut(s) 987, 1012, 1427, 1548, 3013, 3747, 4148
BciVI GTATCC 2 cut(s) 3480, 4199
BclI TGATCA 3 cut(s) 1026, 2359, 4092
BcoDI GTCTC 5 cut(s) 1249, 2025, 2336, 3553, 4271
BfaI CTAG 7 cut(s) 2097, 2546, 2621, 3039, 3443, 4743, 4764
BfmI CTRYAG 2 cut(s) 2462, 4449
BfrI CTTAAG 2 cut(s) 2611, 3968
BfuAI ACCTGC 2 cut(s) 737, 1551
BfuI GTATCC 2 cut(s) 3480, 4199
BglII AGATCT 1 cut(s) 16
BisI GCNGC 7 cut(s) 1858, 2599, 2927, 2979, 3144, 3809, 3884
BlpI GCTNAGC 1 cut(s) 4774
BlsI GCNGC 7 cut(s) 1859, 2600, 2928, 2980, 3145, 3810, 3885
BmcAI AGTACT 2 cut(s) 3966, 4561
Bme1390I CCNGG 7 cut(s) 987, 1012, 1427, 1548, 3013, 3747, 4148
Bme18I GGWCC 6 cut(s) 203, 983, 1423, 2205, 3421, 3494
BmeRI GACNNNNNGTC 1 cut(s) 335
BmeT110I CYCGRG 4 cut(s) 2681, 3668, 3960, 4661
BmgBI CACGTC 1 cut(s) 3976
BmgT120I GGNCC 6 cut(s) 203, 983, 1423, 2205, 3421, 3494
BmiI GGNNCC 6 cut(s) 1962, 2206, 2724, 3201, 3261, 4685
BmrFI CCNGG 7 cut(s) 987, 1012, 1427, 1548, 3013, 3747, 4148
BoxI GACNNNNGTC 2 cut(s) 2837, 4368
BpiI GAAGAC 3 cut(s) 385, 1125, 2738
BplI GAGNNNNNCTC 2 cut(s) 2568, 2600
BpmI CTGGAG 3 cut(s) 1929, 2207, 2417
Bpu1102I GCTNAGC 1 cut(s) 4774
BpuEI CTTGAG 5 cut(s) 782, 1127, 1697, 2572, 3713
Bsa29I ATCGAT 1 cut(s) 2231
BsaAI YACGTR 1 cut(s) 4136
BsaBI GATNNNNATC 2 cut(s) 360, 936
BsaHI GRCGYC 2 cut(s) 3525, 3567
BsaI GGTCTC 1 cut(s) 4271
BsaWI WCCGGW 4 cut(s) 1736, 2818, 3196, 4182
BsaXI ACNNNNNCTCC 2 cut(s) 3554, 3584
Bsc4I CCNNNNNNNGG 6 cut(s) 1277, 2024, 2616, 2825, 3359, 4276
Bse1I ACTGG 3 cut(s) 344, 2251, 3261
Bse3DI GCAATG 2 cut(s) 4080, 4736
Bse8I GATNNNNATC 2 cut(s) 360, 936
BseAI TCCGGA 1 cut(s) 1736
BseBI CCWGG 7 cut(s) 987, 1012, 1427, 1548, 3013, 3747, 4148
BseCI ATCGAT 1 cut(s) 2231
BseJI GATNNNNATC 2 cut(s) 360, 936
BseLI CCNNNNNNNGG 6 cut(s) 1277, 2024, 2616, 2825, 3359, 4276
BseMI GCAATG 2 cut(s) 4080, 4736
BseNI ACTGG 3 cut(s) 344, 2251, 3261
BseRI GAGGAG 3 cut(s) 27, 36, 39
BseSI GKGCMC 3 cut(s) 1793, 2727, 4795
BseXI GCAGC 5 cut(s) 1869, 2610, 2913, 3795, 3895
BsgI GTGCAG 1 cut(s) 1638
Bsh1236I CGCG 4 cut(s) 1352, 3146, 4027, 4699
BshFI GGCC 3 cut(s) 1179, 3116, 3982
BshNI GGYRCC 1 cut(s) 2722
BshVI ATCGAT 1 cut(s) 2231
BsiHKAI GWGCWC 3 cut(s) 396, 1095, 4033
BsiHKCI CYCGRG 4 cut(s) 2681, 3668, 3960, 4661
BsiSI CCGG 4 cut(s) 1737, 2819, 3197, 4183
BslFI GGGAC 6 cut(s) 342, 2218, 2855, 3752, 3943, 4507
BslI CCNNNNNNNGG 6 cut(s) 1277, 2024, 2616, 2825, 3359, 4276
BsmAI GTCTC 5 cut(s) 1249, 2025, 2336, 3553, 4271
BsmBI CGTCTC 1 cut(s) 2025
BsmFI GGGAC 6 cut(s) 342, 2218, 2855, 3752, 3943, 4507
BsmI GAATGC 3 cut(s) 317, 1069, 1538
BsnI GGCC 3 cut(s) 1179, 3116, 3982
Bso31I GGTCTC 1 cut(s) 4271
BsoBI CYCGRG 4 cut(s) 2681, 3668, 3960, 4661
Bsp1286I GDGCHC 7 cut(s) 396, 1095, 1793, 2727, 3746, 4033, 4795
Bsp13I TCCGGA 1 cut(s) 1736
Bsp1407I TGTACA 2 cut(s) 856, 1432
Bsp1720I GCTNAGC 1 cut(s) 4774
Bsp19I CCATGG 1 cut(s) 3414
BspACI CCGC 8 cut(s) 135, 427, 2979, 3144, 3295, 3655, 4025, 4697
BspANI GGCC 3 cut(s) 1179, 3116, 3982
BspDI ATCGAT 1 cut(s) 2231
BspEI TCCGGA 1 cut(s) 1736
BspFNI CGCG 4 cut(s) 1352, 3146, 4027, 4699
BspHI TCATGA 2 cut(s) 4089, 4216
BspLI GGNNCC 6 cut(s) 1962, 2206, 2724, 3201, 3261, 4685
BspMI ACCTGC 2 cut(s) 737, 1551
BspPI GGATC 9 cut(s) 356, 945, 1070, 1213, 1386, 2923, 3194, 3207, 3310
BspT107I GGYRCC 1 cut(s) 2722
BspTI CTTAAG 2 cut(s) 2611, 3968
BspTNI GGTCTC 1 cut(s) 4271
BsrBI CCGCTC 1 cut(s) 137
BsrDI GCAATG 2 cut(s) 4080, 4736
BsrGI TGTACA 2 cut(s) 856, 1432
BsrI ACTGG 3 cut(s) 344, 2251, 3261
BssNAI GTATAC 1 cut(s) 1054
BssNI GRCGYC 2 cut(s) 3525, 3567
BssSI CACGAG 2 cut(s) 3650, 4526
BssT1I CCWWGG 5 cut(s) 1152, 3165, 3414, 3630, 4438
Bst1107I GTATAC 1 cut(s) 1054
Bst2BI CACGAG 2 cut(s) 3650, 4526
Bst2UI CCWGG 7 cut(s) 987, 1012, 1427, 1548, 3013, 3747, 4148
Bst4CI ACNGT 8 cut(s) 627, 1418, 1770, 2466, 2839, 3357, 3379, 3707
Bst6I CTCTTC 2 cut(s) 1247, 3789
BstACI GRCGYC 2 cut(s) 3525, 3567
BstAFI CTTAAG 2 cut(s) 2611, 3968
BstAPI GCANNNNNTGC 1 cut(s) 305
BstAUI TGTACA 2 cut(s) 856, 1432
BstBAI YACGTR 1 cut(s) 4136
BstC8I GCNNGC 4 cut(s) 1898, 2183, 3590, 4029
BstDSI CCRYGG 2 cut(s) 3414, 4307
BstENI CCTNNNNNAGG 2 cut(s) 1275, 2614
BstFNI CGCG 4 cut(s) 1352, 3146, 4027, 4699
BstHHI GCGC 2 cut(s) 3860, 4701
BstMAI GTCTC 5 cut(s) 1249, 2025, 2336, 3553, 4271
BstNI CCWGG 7 cut(s) 987, 1012, 1427, 1548, 3013, 3747, 4148
BstNSI RCATGY 7 cut(s) 411, 588, 1849, 1900, 2933, 2963, 4128
BstPAI GACNNNNGTC 2 cut(s) 2837, 4368
BstSCI CCNGG 7 cut(s) 985, 1010, 1425, 1546, 3011, 3745, 4146
BstSFI CTRYAG 2 cut(s) 2462, 4449
BstSLI GKGCMC 3 cut(s) 1793, 2727, 4795
BstUI CGCG 4 cut(s) 1352, 3146, 4027, 4699
BstV1I GCAGC 5 cut(s) 1869, 2610, 2913, 3795, 3895
BstV2I GAAGAC 3 cut(s) 385, 1125, 2738
BstX2I RGATCY 3 cut(s) 16, 2915, 3199
BstXI CCANNNNNNTGG 3 cut(s) 1595, 3299, 3431
BstYI RGATCY 3 cut(s) 16, 2915, 3199
BstZ17I GTATAC 1 cut(s) 1054
Bsu15I ATCGAT 1 cut(s) 2231
BsuI GTATCC 2 cut(s) 3480, 4199
BsuRI GGCC 3 cut(s) 1179, 3116, 3982
BsuTUI ATCGAT 1 cut(s) 2231
BtgI CCRYGG 2 cut(s) 3414, 4307
BtgZI GCGATG 1 cut(s) 1333
BtrI CACGTC 1 cut(s) 3976
BtsI GCAGTG 1 cut(s) 4785
BveI ACCTGC 2 cut(s) 737, 1551
Cac8I GCNNGC 4 cut(s) 1898, 2183, 3590, 4029
CciI TCATGA 2 cut(s) 4089, 4216
CfoI GCGC 2 cut(s) 3860, 4701
Cfr13I GGNCC 6 cut(s) 203, 983, 1423, 2205, 3421, 3494
ClaI ATCGAT 1 cut(s) 2231
CseI GACGC 3 cut(s) 476, 3533, 3575
CspCI CAANNNNNGTGG 2 cut(s) 1986, 2021
DraI TTTAAA 2 cut(s) 433, 1363
DriI GACNNNNNGTC 1 cut(s) 335
EaeI YGGCCR 1 cut(s) 3114
Eam1104I CTCTTC 2 cut(s) 1247, 3789
Eam1105I GACNNNNNGTC 1 cut(s) 335
EarI CTCTTC 2 cut(s) 1247, 3789
Ecl136II GAGCTC 1 cut(s) 1093
Eco130I CCWWGG 5 cut(s) 1152, 3165, 3414, 3630, 4438
Eco24I GRGCYC 2 cut(s) 1095, 3746
Eco31I GGTCTC 1 cut(s) 4271
Eco32I GATATC 2 cut(s) 1751, 3181
Eco47I GGWCC 6 cut(s) 203, 983, 1423, 2205, 3421, 3494
Eco53kI GAGCTC 1 cut(s) 1093
Eco57I CTGAAG 7 cut(s) 498, 540, 1101, 2054, 2165, 2841, 4725
Eco88I CYCGRG 4 cut(s) 2681, 3668, 3960, 4661
EcoICRI GAGCTC 1 cut(s) 1093
EcoNI CCTNNNNNAGG 2 cut(s) 1275, 2614
EcoRII CCWGG 7 cut(s) 985, 1010, 1425, 1546, 3011, 3745, 4146
EcoRV GATATC 2 cut(s) 1751, 3181
EcoT14I CCWWGG 5 cut(s) 1152, 3165, 3414, 3630, 4438
EcoT22I ATGCAT 4 cut(s) 317, 1069, 1491, 4644
EcoT38I GRGCYC 2 cut(s) 1095, 3746
ErhI CCWWGG 5 cut(s) 1152, 3165, 3414, 3630, 4438
Esp3I CGTCTC 1 cut(s) 2025
FalI AAGNNNNNCTT 6 cut(s) 456, 488, 807, 839, 4191, 4223
FaqI GGGAC 6 cut(s) 342, 2218, 2855, 3752, 3943, 4507
FauNDI CATATG 1 cut(s) 3315
FbaI TGATCA 3 cut(s) 1026, 2359, 4092
FblI GTMKAC 4 cut(s) 1053, 1600, 2707, 4801
Fnu4HI GCNGC 7 cut(s) 1858, 2599, 2927, 2979, 3144, 3809, 3884
FriOI GRGCYC 2 cut(s) 1095, 3746
Fsp4HI GCNGC 7 cut(s) 1858, 2599, 2927, 2979, 3144, 3809, 3884
FspAI RTGCGCAY 1 cut(s) 3859
FspBI CTAG 7 cut(s) 2097, 2546, 2621, 3039, 3443, 4743, 4764
FspI TGCGCA 1 cut(s) 3859
GlaI GCGC 2 cut(s) 3859, 4700
GluI GCNGC 7 cut(s) 1858, 2599, 2927, 2979, 3144, 3809, 3884
GsuI CTGGAG 3 cut(s) 1929, 2207, 2417
HaeIII GGCC 3 cut(s) 1179, 3116, 3982
HapII CCGG 4 cut(s) 1737, 2819, 3197, 4183
HgaI GACGC 3 cut(s) 476, 3533, 3575
HhaI GCGC 2 cut(s) 3860, 4701
Hin1I GRCGYC 2 cut(s) 3525, 3567
Hin6I GCGC 2 cut(s) 3858, 4699
HinP1I GCGC 2 cut(s) 3858, 4699
HincII GTYRAC 2 cut(s) 1601, 1941
HindII GTYRAC 2 cut(s) 1601, 1941
HindIII AAGCTT 5 cut(s) 1083, 1999, 2179, 2402, 2732
HpaII CCGG 4 cut(s) 1737, 2819, 3197, 4183
HphI GGTGA 6 cut(s) 605, 761, 1072, 2006, 2808, 3394
Hpy99I CGWCG 4 cut(s) 1605, 1615, 3527, 3569
HpyAV CCTTC 8 cut(s) 33, 51, 1190, 2093, 2159, 2610, 4396, 4805
HpyCH4III ACNGT 8 cut(s) 627, 1418, 1770, 2466, 2839, 3357, 3379, 3707
HpyCH4IV ACGT 6 cut(s) 1337, 1555, 1603, 2754, 3975, 4135
HpySE526I ACGT 6 cut(s) 1337, 1555, 1603, 2754, 3975, 4135
Hsp92I GRCGYC 2 cut(s) 3525, 3567
HspAI GCGC 2 cut(s) 3858, 4699
Kpn2I TCCGGA 1 cut(s) 1736
Ksp22I TGATCA 3 cut(s) 1026, 2359, 4092
LmnI GCTCC 6 cut(s) 391, 1040, 1893, 2188, 2354, 2576
Lsp1109I GCAGC 5 cut(s) 1869, 2610, 2913, 3795, 3895
MaeI CTAG 7 cut(s) 2097, 2546, 2621, 3039, 3443, 4743, 4764
MaeII ACGT 6 cut(s) 1337, 1555, 1603, 2754, 3975, 4135
MaeIII GTNAC 6 cut(s) 1406, 1459, 1764, 2830, 3919, 4131
MbiI CCGCTC 1 cut(s) 137
MfeI CAATTG 5 cut(s) 198, 222, 950, 1671, 2717
MflI RGATCY 3 cut(s) 16, 2915, 3199
MhlI GDGCHC 7 cut(s) 396, 1095, 1793, 2727, 3746, 4033, 4795
MlyI GAGTC 7 cut(s) 2831, 2877, 3590, 3680, 3806, 4595, 4672
MmeI TCCRAC 2 cut(s) 1108, 1843
Mph1103I ATGCAT 4 cut(s) 317, 1069, 1491, 4644
MroI TCCGGA 1 cut(s) 1736
MroXI GAANNNNTTC 4 cut(s) 1137, 1521, 4418, 4475
MslI CAYNNNNRTG 4 cut(s) 618, 2199, 3650, 4788
MspCI CTTAAG 2 cut(s) 2611, 3968
MspI CCGG 4 cut(s) 1737, 2819, 3197, 4183
MspR9I CCNGG 7 cut(s) 987, 1012, 1427, 1548, 3013, 3747, 4148
MunI CAATTG 5 cut(s) 198, 222, 950, 1671, 2717
Mva1269I GAATGC 3 cut(s) 317, 1069, 1538
MvaI CCWGG 7 cut(s) 987, 1012, 1427, 1548, 3013, 3747, 4148
MvnI CGCG 4 cut(s) 1352, 3146, 4027, 4699
NcoI CCATGG 1 cut(s) 3414
NdeI CATATG 1 cut(s) 3315
NlaIV GGNNCC 6 cut(s) 1962, 2206, 2724, 3201, 3261, 4685
NmeAIII GCCGAG 1 cut(s) 2504
NmuCI GTSAC 3 cut(s) 1406, 2830, 4131
NsbI TGCGCA 1 cut(s) 3859
NsiI ATGCAT 4 cut(s) 317, 1069, 1491, 4644
NspI RCATGY 7 cut(s) 411, 588, 1849, 1900, 2933, 2963, 4128
OliI CACNNNNGTG 1 cut(s) 3650
PaeI GCATGC 1 cut(s) 1900
PaeR7I CTCGAG 3 cut(s) 2681, 3668, 4661
PagI TCATGA 2 cut(s) 4089, 4216
PaqCI CACCTGC 1 cut(s) 737
PasI CCCWGGG 1 cut(s) 3746
PciI ACATGT 4 cut(s) 584, 1845, 2959, 4124
PcsI WCGNNNNNNNCGW 1 cut(s) 3570
PctI GAATGC 3 cut(s) 317, 1069, 1538
PdmI GAANNNNTTC 4 cut(s) 1137, 1521, 4418, 4475
PflFI GACNNNGTC 1 cut(s) 3545
PkrI GCNGC 7 cut(s) 1859, 2600, 2928, 2980, 3145, 3810, 3885
PleI GAGTC 7 cut(s) 2830, 2876, 3589, 3679, 3805, 4595, 4672
PpsI GAGTC 7 cut(s) 2830, 2876, 3589, 3679, 3805, 4595, 4672
Ppu21I YACGTR 1 cut(s) 4136
PscI ACATGT 4 cut(s) 584, 1845, 2959, 4124
PshAI GACNNNNGTC 2 cut(s) 2837, 4368
PshBI ATTAAT 3 cut(s) 1911, 4064, 4653
Psp124BI GAGCTC 1 cut(s) 1095
Psp1406I AACGTT 1 cut(s) 1337
Psp6I CCWGG 7 cut(s) 985, 1010, 1425, 1546, 3011, 3745, 4146
PspGI CCWGG 7 cut(s) 985, 1010, 1425, 1546, 3011, 3745, 4146
PspN4I GGNNCC 6 cut(s) 1962, 2206, 2724, 3201, 3261, 4685
PspPI GGNCC 6 cut(s) 203, 983, 1423, 2205, 3421, 3494
PspXI VCTCGAGB 3 cut(s) 2681, 3668, 4661
PsuI RGATCY 3 cut(s) 16, 2915, 3199
PsyI GACNNNGTC 1 cut(s) 3545
RseI CAYNNNNRTG 4 cut(s) 618, 2199, 3650, 4788
SacI GAGCTC 1 cut(s) 1095
SalI GTCGAC 1 cut(s) 1599
SatI GCNGC 7 cut(s) 1858, 2599, 2927, 2979, 3144, 3809, 3884
Sau96I GGNCC 6 cut(s) 203, 983, 1423, 2205, 3421, 3494
ScaI AGTACT 2 cut(s) 3966, 4561
SchI GAGTC 7 cut(s) 2831, 2877, 3590, 3680, 3806, 4595, 4672
ScrFI CCNGG 7 cut(s) 987, 1012, 1427, 1548, 3013, 3747, 4148
SduI GDGCHC 7 cut(s) 396, 1095, 1793, 2727, 3746, 4033, 4795
SfcI CTRYAG 2 cut(s) 2462, 4449
Sfr274I CTCGAG 3 cut(s) 2681, 3668, 4661
SinI GGWCC 6 cut(s) 203, 983, 1423, 2205, 3421, 3494
SlaI CTCGAG 3 cut(s) 2681, 3668, 4661
SmiMI CAYNNNNRTG 4 cut(s) 618, 2199, 3650, 4788
SphI GCATGC 1 cut(s) 1900
SsiI CCGC 8 cut(s) 135, 427, 2979, 3144, 3295, 3655, 4025, 4697
SspMI CTAG 7 cut(s) 2097, 2546, 2621, 3039, 3443, 4743, 4764
SstI GAGCTC 1 cut(s) 1095
StyD4I CCNGG 7 cut(s) 985, 1010, 1425, 1546, 3011, 3745, 4146
StyI CCWWGG 5 cut(s) 1152, 3165, 3414, 3630, 4438
TaaI ACNGT 8 cut(s) 627, 1418, 1770, 2466, 2839, 3357, 3379, 3707
TaiI ACGT 6 cut(s) 1340, 1558, 1606, 2757, 3978, 4138
TauI GCSGC 2 cut(s) 2981, 3146
TseFI GTSAC 3 cut(s) 1406, 2830, 4131
TseI GCWGC 5 cut(s) 1857, 2598, 2926, 3808, 3883
Tsp45I GTSAC 3 cut(s) 1406, 2830, 4131
TspGWI ACGGA 4 cut(s) 2727, 2798, 3428, 3562
Tth111I GACNNNGTC 1 cut(s) 3545
Vha464I CTTAAG 2 cut(s) 2611, 3968
VpaK11BI GGWCC 6 cut(s) 203, 983, 1423, 2205, 3421, 3494
VspI ATTAAT 3 cut(s) 1911, 4064, 4653
XagI CCTNNNNNAGG 2 cut(s) 1275, 2614
XceI RCATGY 7 cut(s) 411, 588, 1849, 1900, 2933, 2963, 4128
XcmI CCANNNNNNNNNTGG 1 cut(s) 3412
XhoI CTCGAG 3 cut(s) 2681, 3668, 4661
XmiI GTMKAC 4 cut(s) 1053, 1600, 2707, 4801
XmnI GAANNNNTTC 4 cut(s) 1137, 1521, 4418, 4475
XspI CTAG 7 cut(s) 2097, 2546, 2621, 3039, 3443, 4743, 4764
ZrmI AGTACT 2 cut(s) 3966, 4561
Zsp2I ATGCAT 4 cut(s) 317, 1069, 1491, 4644
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.