RLG00000000666

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000129
Physical Location & Seq
Reverse (-)
3421158 .. 3424428
3271 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000666

Sequence Viewer

Length: 2505 bp
ATGTCGACTCCAGACTTCACGGAGGTTCCTGATCTTGAAAAGTTAGTGCTTCAAGATTGCACAAGGTTGGTGAAGATTCACCCATCTCTTGGATTTCTGAAGAAACTTATCTCTTTGAACATGAGAAATTGCAAGTCTATTGAGAGCATTCCACCTTTCACTACCTTGGAATCCCTTCAAAAATTGAGGCTTTCATTCTGTTCAGGACTGAAGAAGTTTCCAGAAATTGAAGGAAATATGAAAAGCTTGCTGAAGCTTCATTTGGATGGGACCAGCATTGAGGAATTGTCTCCATCAATTGAACGCTTGACTGGTCTTGCCCTGTTGAATCTGACAGATTGTAAAAACCTTTTGCATCTTCCCCATACCATTGGGTGCTTGACATCTTTGAAAAGTCTCTTTCTAACTGGTTGCTCCAAAATTGATGAGATACCCGAGAATCTGAATGGTATGAAATGTCTGGAGACGCTTGCAATTGGTGGAACTTCCATAAGGGAGTTATCTTTCATTCTAGGCATGAAGAATCTGAAGTATATATCCTGCCAAGGATGTACATGTCTAGCTCCAGAATCATGCAAAGTTCTGGCTTCGTTATCGAATTTGATAGAGCTAGACTTGAGTTATTGCAATCTAATGGATGGAGCATTTCTCAATGAGTCTAGCAGCCTAATCTCCTTAACATTGTTAGATTTAAGTGGTAATTGCTTTGAGCGTTTCCGGTCAGATTTCAGTGGCGTAGATCCTAGAGATGAAATTCCAGAGTGGTTCGGCACCATATCCACCAGAAATGAAATATCAATCCCGATACCTCAAAATCTGGCCGATGACAACGAGTGGGTGGGATTAGCCGCGTGTGCTATATTCTCAGTCAAGGGGCATACTGCTCTCTCCCTTATTGAACCGGATCCTGATTTTCTTAATTACTTGTATCAAATAACATTGGAAACTGATGTTATGCGTCTGAAACCATATGTGTTCCGCGTCTCAGCGCGCCCCCTAGGATCAAGTTCTCATCTGCTTGTGATTTTCCATATACCTCATCGGCAGGTTCCATACGGGGAGTTAAATAAATCGACTGTGGTGAAGGCTATATTTGAAACCACCAATCCATCCATGGTGGTCCAGAAATGTGGAATTCGTCTACTCTATGAGCAAGATGCTAAATTGTTTTCCAGCATAGTAGGGGTGGATCATCCGACTGGGCTGACAGAAGTTGAGGACGCCGCCTCTGCGGAGTCTGGCTGGCTTAGTCAATCTTTGGAACGGGAGAAATTCATTCCACCCTTGGAAGAAGAATCCACACTTGTGCTGAGAAAGAACCTTGAGTCTGTTCTTCCAAGATTCCTTGAGGCACTAAACAGTTATTCTAAAATATTCAAATTTAACCTCAAAGGAAGCCCAGGGTGGTTTGAGTTGTTTCACCTCGATGCGGGGTCAAAATTGAACCTAATTCCAATCAAGCTGCCTCAGAATCCACATAAGAGCAAGAAGTGGATGGGATTTGCAATGTGCGCATCGCTTGTAGAGGAAGTGGGGCAGACAATGAAAGAGGACAACTATTGGGTAATTGTTAAACTATTTGGTACGGGAATGGCCCAAGTACTTAAGCACGTCAGGCCATTGTCAGAAGAACATCATCAACTATTGGTTATTTACATACCGCGGGCACAGATTCCTGAAGTGTCGTTCACTCAGACATTAAAAACTGAAATGGACATTGGCATTGTGGTCCATAGCCCTCATGTAAAGGTTCAAAGAAGTGGGCTCCGTAGTGTGTACCAGGAAGATATACAAGGGTTTGCTGATACAATTATCCGGTGCATGCAAAGGGAAGATTCTCTTAAATCTTACGATAAGTTGGTGGTAAAAGAATGGATAAATTTGATACAATTGCAAGGTGCCCTTTCATTAGAGAGAGTGACAGAACGGGATTCCAGAACCCCATGGGAAAAATACTTTGAATTGCACTCACGAAAATATAGAAACTGGGACTGGTCTGACCCTCGTCCAGTAATTTGTTGTGGCTTCAAGAGAGCTGAAATTTACAAGTGGAAATGGTTCATGCCTTTCATCAACCAAGGCAACTCTGCAGAAATCCAACTGCCTCTGAATCTGTTCGATGATGATAATTGGTTGGGGTTTGCCGTATGTTTTCAGTTGTCCCACGACCAATATCCAAACATTAGTCATGGCAATATCGCTCTCGATTCAGAGAATGTTGTGCTTAATTATCGTCTGGACTCTGATGTTGCTTGGCACGGCTTATTTGATTTCATGATGCATGCAGATATTAAGATACCCGAGCAATCGGAATACTACGCGACTCATTTCCTTTATATACCGCGCACTAGAGTTAGCAAGATGTGGAGGCAATGCAAACTGGCCAGGATAACTTTGTGGTTCTCTAGCTCATGCTTAGCAGTGCATAGTTGTGCCCTTGGTCTACTATTTAAAGACGACGTTGAACATCTTGTAGAAACACTGGTCCTCGCCGAATTACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

835

Amino Acids

94.78

Weight (kDa)

5.93

Isoelectric Point (pI)

37.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 27 - 135 1.2e-06 Leucine-rich repeat region
LRR_13 PF23286 56 - 143 8.4e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 139 - 231 9.2e-06 Leucine-rich repeat region
C-JID PF20160 248 - 387 1.3e-08 C-JID domain
C-JID PF20160 484 - 597 4.5e-07 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1513
Acc36I ACCTGC 1 cut(s) 1036
AccB1I GGYRCC 2 cut(s) 770, 1898
AccB7I CCANNNNNTGG 1 cut(s) 89
AccI GTMKAC 3 cut(s) 5, 1141, 2443
AccII CGCG 6 cut(s) 851, 981, 991, 1663, 2321, 2344
AciI CCGC 8 cut(s) 849, 979, 1224, 1232, 1430, 1661, 1663, 2342
AclWI GGATC 5 cut(s) 734, 899, 912, 1009, 1197
AcoI YGGCCR 2 cut(s) 819, 2382
AcsI RAATTY 7 cut(s) 598, 753, 1134, 1271, 1379, 1879, 2040
AcuI CTGAAG 5 cut(s) 119, 230, 272, 548, 1698
AcyI GRCGYC 1 cut(s) 1221
AfaI GTAC 4 cut(s) 553, 1585, 1602, 1778
AfiI CCNNNNNNNGG 2 cut(s) 89, 1429
AflII CTTAAG 1 cut(s) 1604
AflIII ACRYGT 1 cut(s) 554
AjiI CACGTC 1 cut(s) 1612
AjnI CCWGG 3 cut(s) 1399, 1779, 2384
AjuI GAANNNNNNNTTGG 4 cut(s) 245, 277, 1701, 1733
AleI CACNNNNGTG 1 cut(s) 1304
AluBI AGCT 7 cut(s) 246, 256, 563, 610, 1462, 2036, 2409
AluI AGCT 7 cut(s) 246, 256, 563, 610, 1462, 2036, 2409
Alw26I GTCTC 4 cut(s) 294, 401, 458, 988
AlwI GGATC 5 cut(s) 734, 899, 912, 1009, 1197
Ama87I CYCGRG 2 cut(s) 434, 2300
AoxI GGCC 4 cut(s) 819, 1593, 1616, 2382
ApeKI GCWGC 2 cut(s) 663, 1462
ApoI RAATTY 7 cut(s) 598, 753, 1134, 1271, 1379, 1879, 2040
Asp700I GAANNNNTTC 3 cut(s) 174, 2057, 2114
AspA2I CCTAGG 1 cut(s) 997
AspLEI GCGC 4 cut(s) 991, 993, 1514, 2346
AspS9I GGNCC 5 cut(s) 270, 1120, 1594, 1729, 2485
AsuHPI GGTGA 4 cut(s) 71, 82, 1093, 1412
AvaI CYCGRG 2 cut(s) 434, 2300
AvaII GGWCC 4 cut(s) 270, 1120, 1729, 2485
AvrII CCTAGG 1 cut(s) 997
BaeGI GKGCMC 3 cut(s) 1669, 1903, 2437
BalI TGGCCA 1 cut(s) 2384
BamHI GGATCC 1 cut(s) 904
BanI GGYRCC 2 cut(s) 770, 1898
BanII GRGCYC 1 cut(s) 1767
BbvI GCAGC 2 cut(s) 675, 1449
BccI CCATC 6 cut(s) 91, 260, 301, 632, 1117, 1489
BceAI ACGGC 2 cut(s) 2129, 2275
BciT130I CCWGG 3 cut(s) 1401, 1781, 2386
BcoDI GTCTC 4 cut(s) 294, 401, 458, 988
BfaI CTAG 8 cut(s) 512, 560, 611, 660, 744, 998, 2349, 2406
BfmI CTRYAG 1 cut(s) 2088
BfrI CTTAAG 1 cut(s) 1604
BfuAI ACCTGC 1 cut(s) 1036
BisI GCNGC 4 cut(s) 664, 849, 1224, 1463
BlnI CCTAGG 1 cut(s) 997
BlpI GCTNAGC 1 cut(s) 2416
BlsI GCNGC 4 cut(s) 665, 850, 1225, 1464
BmcAI AGTACT 1 cut(s) 1602
Bme1390I CCNGG 3 cut(s) 1401, 1781, 2386
Bme18I GGWCC 4 cut(s) 270, 1120, 1729, 2485
BmeT110I CYCGRG 2 cut(s) 434, 2300
BmgBI CACGTC 1 cut(s) 1612
BmgT120I GGNCC 5 cut(s) 270, 1120, 1594, 1729, 2485
BmiI GGNNCC 7 cut(s) 27, 271, 772, 906, 1050, 1766, 1900
BmrFI CCNGG 3 cut(s) 1401, 1781, 2386
BmrI ACTGGG 2 cut(s) 1209, 1996
BmsI GCATC 5 cut(s) 364, 1147, 1417, 1523, 2268
BmuI ACTGGG 2 cut(s) 1209, 1996
BoxI GACNNNNGTC 1 cut(s) 2004
BplI GAGNNNNNCTC 2 cut(s) 633, 665
BpmI CTGGAG 2 cut(s) 482, 549
Bpu1102I GCTNAGC 1 cut(s) 2416
BpuEI CTTGAG 3 cut(s) 637, 1343, 1367
BsaHI GRCGYC 1 cut(s) 1221
BsaWI WCCGGW 3 cut(s) 717, 901, 1815
Bsc4I CCNNNNNNNGG 2 cut(s) 89, 1429
Bse1I ACTGG 8 cut(s) 316, 412, 1204, 1991, 1997, 2009, 2385, 2487
Bse3DI GCAATG 2 cut(s) 1512, 2378
BseBI CCWGG 3 cut(s) 1401, 1781, 2386
BseGI GGATG 6 cut(s) 271, 554, 643, 1109, 1192, 1500
BseLI CCNNNNNNNGG 2 cut(s) 89, 1429
BseMI GCAATG 2 cut(s) 1512, 2378
BseMII CTCAG 5 cut(s) 879, 999, 1301, 1481, 1706
BseNI ACTGG 8 cut(s) 316, 412, 1204, 1991, 1997, 2009, 2385, 2487
BsePI GCGCGC 1 cut(s) 989
BseSI GKGCMC 3 cut(s) 1669, 1903, 2437
BseXI GCAGC 2 cut(s) 675, 1449
Bsh1236I CGCG 6 cut(s) 851, 981, 991, 1663, 2321, 2344
BshFI GGCC 4 cut(s) 821, 1595, 1618, 2384
BshNI GGYRCC 2 cut(s) 770, 1898
BsiHKCI CYCGRG 2 cut(s) 434, 2300
BsiSI CCGG 3 cut(s) 718, 902, 1816
BslFI GGGAC 3 cut(s) 283, 2003, 2146
BslI CCNNNNNNNGG 2 cut(s) 89, 1429
BsmAI GTCTC 4 cut(s) 294, 401, 458, 988
BsmBI CGTCTC 2 cut(s) 458, 988
BsmFI GGGAC 3 cut(s) 283, 2003, 2146
BsmI GAATGC 1 cut(s) 147
BsnI GGCC 4 cut(s) 821, 1595, 1618, 2384
BsoBI CYCGRG 2 cut(s) 434, 2300
Bsp1286I GDGCHC 4 cut(s) 1669, 1767, 1903, 2437
Bsp1407I TGTACA 1 cut(s) 551
Bsp143I GATC 5 cut(s) 31, 739, 904, 1001, 1189
Bsp1720I GCTNAGC 1 cut(s) 2416
Bsp19I CCATGG 2 cut(s) 1113, 1943
BspACI CCGC 8 cut(s) 849, 979, 1224, 1232, 1430, 1661, 1663, 2342
BspANI GGCC 4 cut(s) 821, 1595, 1618, 2384
BspCNI CTCAG 5 cut(s) 878, 998, 1302, 1480, 1705
BspFNI CGCG 6 cut(s) 851, 981, 991, 1663, 2321, 2344
BspHI TCATGA 1 cut(s) 2274
BspLI GGNNCC 7 cut(s) 27, 271, 772, 906, 1050, 1766, 1900
BspMAI CTGCAG 1 cut(s) 2092
BspMI ACCTGC 1 cut(s) 1036
BspPI GGATC 5 cut(s) 734, 899, 912, 1009, 1197
BspT107I GGYRCC 2 cut(s) 770, 1898
BspTI CTTAAG 1 cut(s) 1604
BsrDI GCAATG 2 cut(s) 1512, 2378
BsrGI TGTACA 1 cut(s) 551
BsrI ACTGG 8 cut(s) 316, 412, 1204, 1991, 1997, 2009, 2385, 2487
BssHII GCGCGC 1 cut(s) 989
BssMI GATC 5 cut(s) 31, 739, 904, 1001, 1189
BssNI GRCGYC 1 cut(s) 1221
BssT1I CCWWGG 8 cut(s) 165, 544, 997, 1113, 1284, 1943, 2077, 2437
Bst2UI CCWGG 3 cut(s) 1401, 1781, 2386
Bst4CI ACNGT 2 cut(s) 1078, 1361
BstACI GRCGYC 1 cut(s) 1221
BstAFI CTTAAG 1 cut(s) 1604
BstAUI TGTACA 1 cut(s) 551
BstC8I GCNNGC 7 cut(s) 248, 471, 991, 1244, 1665, 1823, 2283
BstDEI CTNAG 7 cut(s) 865, 985, 1247, 1310, 1467, 1692, 2416
BstDSI CCRYGG 3 cut(s) 1113, 1661, 1943
BstF5I GGATG 6 cut(s) 271, 554, 643, 1109, 1192, 1500
BstFNI CGCG 6 cut(s) 851, 981, 991, 1663, 2321, 2344
BstHHI GCGC 4 cut(s) 991, 993, 1514, 2346
BstKTI GATC 5 cut(s) 34, 742, 907, 1004, 1192
BstMAI GTCTC 4 cut(s) 294, 401, 458, 988
BstMBI GATC 5 cut(s) 31, 739, 904, 1001, 1189
BstMWI GCNNNNNNNGC 4 cut(s) 854, 1229, 1511, 1615
BstNI CCWGG 3 cut(s) 1401, 1781, 2386
BstNSI RCATGY 3 cut(s) 558, 1825, 2285
BstPAI GACNNNNGTC 1 cut(s) 2004
BstSCI CCNGG 3 cut(s) 1399, 1779, 2384
BstSFI CTRYAG 1 cut(s) 2088
BstSLI GKGCMC 3 cut(s) 1669, 1903, 2437
BstUI CGCG 6 cut(s) 851, 981, 991, 1663, 2321, 2344
BstV1I GCAGC 2 cut(s) 675, 1449
BstX2I RGATCY 2 cut(s) 739, 904
BstXI CCANNNNNNTGG 2 cut(s) 371, 1130
BstYI RGATCY 2 cut(s) 739, 904
BsuRI GGCC 4 cut(s) 821, 1595, 1618, 2384
BtgI CCRYGG 3 cut(s) 1113, 1661, 1943
BtgZI GCGATG 1 cut(s) 1500
BtrI CACGTC 1 cut(s) 1612
BtsCI GGATG 6 cut(s) 271, 554, 643, 1109, 1192, 1500
BtsI GCAGTG 1 cut(s) 2427
BtsIMutI CAGTG 3 cut(s) 736, 2427, 2480
BveI ACCTGC 1 cut(s) 1036
Cac8I GCNNGC 7 cut(s) 248, 471, 991, 1244, 1665, 1823, 2283
CciI TCATGA 1 cut(s) 2274
CfoI GCGC 4 cut(s) 991, 993, 1514, 2346
Cfr13I GGNCC 5 cut(s) 270, 1120, 1594, 1729, 2485
Cfr42I CCGCGG 1 cut(s) 1664
CseI GACGC 4 cut(s) 475, 947, 970, 1229
Csp6I GTAC 4 cut(s) 552, 1584, 1601, 1777
CviQI GTAC 4 cut(s) 552, 1584, 1601, 1777
DdeI CTNAG 7 cut(s) 865, 985, 1247, 1310, 1467, 1692, 2416
DpnI GATC 5 cut(s) 33, 741, 906, 1003, 1191
DpnII GATC 5 cut(s) 31, 739, 904, 1001, 1189
DraI TTTAAA 1 cut(s) 2452
EaeI YGGCCR 2 cut(s) 819, 2382
Eco130I CCWWGG 8 cut(s) 165, 544, 997, 1113, 1284, 1943, 2077, 2437
Eco24I GRGCYC 1 cut(s) 1767
Eco47I GGWCC 4 cut(s) 270, 1120, 1729, 2485
Eco57I CTGAAG 5 cut(s) 119, 230, 272, 548, 1698
Eco88I CYCGRG 2 cut(s) 434, 2300
EcoRI GAATTC 1 cut(s) 1134
EcoRII CCWGG 3 cut(s) 1399, 1779, 2384
EcoT14I CCWWGG 8 cut(s) 165, 544, 997, 1113, 1284, 1943, 2077, 2437
EcoT22I ATGCAT 1 cut(s) 2283
EcoT38I GRGCYC 1 cut(s) 1767
ErhI CCWWGG 8 cut(s) 165, 544, 997, 1113, 1284, 1943, 2077, 2437
Esp3I CGTCTC 2 cut(s) 458, 988
FalI AAGNNNNNCTT 4 cut(s) 1824, 1856, 1887, 1919
FaqI GGGAC 3 cut(s) 283, 2003, 2146
FauI CCCGC 2 cut(s) 1423, 1656
FauNDI CATATG 1 cut(s) 970
FblI GTMKAC 3 cut(s) 5, 1141, 2443
Fnu4HI GCNGC 4 cut(s) 664, 849, 1224, 1463
FokI GGATG 6 cut(s) 278, 561, 650, 1096, 1179, 1507
FriOI GRGCYC 1 cut(s) 1767
Fsp4HI GCNGC 4 cut(s) 664, 849, 1224, 1463
FspAI RTGCGCAY 1 cut(s) 1513
FspBI CTAG 8 cut(s) 512, 560, 611, 660, 744, 998, 2349, 2406
FspI TGCGCA 1 cut(s) 1513
GlaI GCGC 4 cut(s) 990, 992, 1513, 2345
GluI GCNGC 4 cut(s) 664, 849, 1224, 1463
GsuI CTGGAG 2 cut(s) 482, 549
HaeIII GGCC 4 cut(s) 821, 1595, 1618, 2384
HapII CCGG 3 cut(s) 718, 902, 1816
HgaI GACGC 4 cut(s) 475, 947, 970, 1229
HhaI GCGC 4 cut(s) 991, 993, 1514, 2346
Hin1I GRCGYC 1 cut(s) 1221
Hin6I GCGC 4 cut(s) 989, 991, 1512, 2344
HinP1I GCGC 4 cut(s) 989, 991, 1512, 2344
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HindIII AAGCTT 2 cut(s) 244, 254
HpaII CCGG 3 cut(s) 718, 902, 1816
HphI GGTGA 4 cut(s) 71, 82, 1093, 1412
Hpy166II GTNNAC 5 cut(s) 6, 1142, 1689, 1777, 2444
Hpy8I GTNNAC 5 cut(s) 6, 1142, 1689, 1777, 2444
Hpy99I CGWCG 1 cut(s) 2462
HpyAV CCTTC 3 cut(s) 185, 224, 1078
HpyCH4III ACNGT 2 cut(s) 1078, 1361
HpyCH4IV ACGT 2 cut(s) 1611, 2460
HpyF10VI GCNNNNNNNGC 4 cut(s) 854, 1229, 1511, 1615
HpyF3I CTNAG 7 cut(s) 865, 985, 1247, 1310, 1467, 1692, 2416
HpySE526I ACGT 2 cut(s) 1611, 2460
Hsp92I GRCGYC 1 cut(s) 1221
HspAI GCGC 4 cut(s) 989, 991, 1512, 2344
KspI CCGCGG 1 cut(s) 1664
Kzo9I GATC 5 cut(s) 31, 739, 904, 1001, 1189
LmnI GCTCC 4 cut(s) 419, 568, 641, 1770
Lsp1109I GCAGC 2 cut(s) 675, 1449
LweI GCATC 5 cut(s) 364, 1147, 1417, 1523, 2268
MaeI CTAG 8 cut(s) 512, 560, 611, 660, 744, 998, 2349, 2406
MaeII ACGT 2 cut(s) 1611, 2460
MaeIII GTNAC 1 cut(s) 1918
MalI GATC 5 cut(s) 33, 741, 906, 1003, 1191
MboI GATC 5 cut(s) 31, 739, 904, 1001, 1189
MfeI CAATTG 3 cut(s) 297, 474, 1889
MflI RGATCY 2 cut(s) 739, 904
MhlI GDGCHC 4 cut(s) 1669, 1767, 1903, 2437
MlsI TGGCCA 1 cut(s) 2384
MluNI TGGCCA 1 cut(s) 2384
MlyI GAGTC 5 cut(s) 665, 1244, 1334, 2234, 2317
MmeI TCCRAC 2 cut(s) 1220, 2122
Mox20I TGGCCA 1 cut(s) 2384
Mph1103I ATGCAT 1 cut(s) 2283
MroXI GAANNNNTTC 3 cut(s) 174, 2057, 2114
MscI TGGCCA 1 cut(s) 2384
MslI CAYNNNNRTG 4 cut(s) 264, 1304, 1425, 2430
Msp20I TGGCCA 1 cut(s) 2384
MspA1I CMGCKG 1 cut(s) 1663
MspCI CTTAAG 1 cut(s) 1604
MspI CCGG 3 cut(s) 718, 902, 1816
MspR9I CCNGG 3 cut(s) 1401, 1781, 2386
MunI CAATTG 3 cut(s) 297, 474, 1889
Mva1269I GAATGC 1 cut(s) 147
MvaI CCWGG 3 cut(s) 1401, 1781, 2386
MvnI CGCG 6 cut(s) 851, 981, 991, 1663, 2321, 2344
MwoI GCNNNNNNNGC 4 cut(s) 854, 1229, 1511, 1615
NcoI CCATGG 2 cut(s) 1113, 1943
NdeI CATATG 1 cut(s) 970
NdeII GATC 5 cut(s) 31, 739, 904, 1001, 1189
NlaIV GGNNCC 7 cut(s) 27, 271, 772, 906, 1050, 1766, 1900
NmuCI GTSAC 1 cut(s) 1918
NsbI TGCGCA 1 cut(s) 1513
NsiI ATGCAT 1 cut(s) 2283
NspI RCATGY 3 cut(s) 558, 1825, 2285
OliI CACNNNNGTG 1 cut(s) 1304
PaeI GCATGC 2 cut(s) 1825, 2285
PagI TCATGA 1 cut(s) 2274
PasI CCCWGGG 1 cut(s) 1400
PauI GCGCGC 1 cut(s) 989
PciI ACATGT 1 cut(s) 554
PctI GAATGC 1 cut(s) 147
PdmI GAANNNNTTC 3 cut(s) 174, 2057, 2114
PflMI CCANNNNNTGG 1 cut(s) 89
PkrI GCNGC 4 cut(s) 665, 850, 1225, 1464
PleI GAGTC 5 cut(s) 664, 1243, 1333, 2234, 2317
PpsI GAGTC 5 cut(s) 664, 1243, 1333, 2234, 2317
PscI ACATGT 1 cut(s) 554
PshAI GACNNNNGTC 1 cut(s) 2004
Psp6I CCWGG 3 cut(s) 1399, 1779, 2384
PspGI CCWGG 3 cut(s) 1399, 1779, 2384
PspN4I GGNNCC 7 cut(s) 27, 271, 772, 906, 1050, 1766, 1900
PspPI GGNCC 5 cut(s) 270, 1120, 1594, 1729, 2485
PstI CTGCAG 1 cut(s) 2092
PsuI RGATCY 2 cut(s) 739, 904
PteI GCGCGC 1 cut(s) 989
RsaI GTAC 4 cut(s) 553, 1585, 1602, 1778
RsaNI GTAC 4 cut(s) 552, 1584, 1601, 1777
RseI CAYNNNNRTG 4 cut(s) 264, 1304, 1425, 2430
SacII CCGCGG 1 cut(s) 1664
SalI GTCGAC 1 cut(s) 4
SatI GCNGC 4 cut(s) 664, 849, 1224, 1463
Sau3AI GATC 5 cut(s) 31, 739, 904, 1001, 1189
Sau96I GGNCC 5 cut(s) 270, 1120, 1594, 1729, 2485
ScaI AGTACT 1 cut(s) 1602
SchI GAGTC 5 cut(s) 665, 1244, 1334, 2234, 2317
ScrFI CCNGG 3 cut(s) 1401, 1781, 2386
SduI GDGCHC 4 cut(s) 1669, 1767, 1903, 2437
SfaNI GCATC 5 cut(s) 364, 1147, 1417, 1523, 2268
SfcI CTRYAG 1 cut(s) 2088
Sfr303I CCGCGG 1 cut(s) 1664
SgrBI CCGCGG 1 cut(s) 1664
SinI GGWCC 4 cut(s) 270, 1120, 1729, 2485
SmiMI CAYNNNNRTG 4 cut(s) 264, 1304, 1425, 2430
SmlI CTYRAG 4 cut(s) 616, 1322, 1346, 1604
SmoI CTYRAG 4 cut(s) 616, 1322, 1346, 1604
SphI GCATGC 2 cut(s) 1825, 2285
SsiI CCGC 8 cut(s) 849, 979, 1224, 1232, 1430, 1661, 1663, 2342
SspI AATATT 1 cut(s) 1374
SspMI CTAG 8 cut(s) 512, 560, 611, 660, 744, 998, 2349, 2406
StyD4I CCNGG 3 cut(s) 1399, 1779, 2384
StyI CCWWGG 8 cut(s) 165, 544, 997, 1113, 1284, 1943, 2077, 2437
TaaI ACNGT 2 cut(s) 1078, 1361
TaiI ACGT 2 cut(s) 1614, 2463
TaqI TCGA 6 cut(s) 5, 596, 1073, 1425, 2118, 2205
TatI WGTACW 2 cut(s) 551, 1600
TauI GCSGC 2 cut(s) 851, 1226
TscAI CASTG 3 cut(s) 736, 2427, 2487
TseFI GTSAC 1 cut(s) 1918
TseI GCWGC 2 cut(s) 663, 1462
Tsp45I GTSAC 1 cut(s) 1918
TspGWI ACGGA 2 cut(s) 35, 1757
TspRI CASTG 3 cut(s) 736, 2427, 2487
Van91I CCANNNNNTGG 1 cut(s) 89
Vha464I CTTAAG 1 cut(s) 1604
VpaK11BI GGWCC 4 cut(s) 270, 1120, 1729, 2485
XapI RAATTY 7 cut(s) 598, 753, 1134, 1271, 1379, 1879, 2040
XceI RCATGY 3 cut(s) 558, 1825, 2285
XcmI CCANNNNNNNNNTGG 1 cut(s) 1111
XmaJI CCTAGG 1 cut(s) 997
XmiI GTMKAC 3 cut(s) 5, 1141, 2443
XmnI GAANNNNTTC 3 cut(s) 174, 2057, 2114
XspI CTAG 8 cut(s) 512, 560, 611, 660, 744, 998, 2349, 2406
ZrmI AGTACT 1 cut(s) 1602
Zsp2I ATGCAT 1 cut(s) 2283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.