Rw7G041600

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000129
Physical Location & Seq
Forward (+)
65014536 .. 65020535
6000 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G041600.1

Sequence Viewer

Length: 4839 bp
ATGACTACCAAAATAGATCTTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTGTCCTCCTCTTTCTCTATTTCGTAAATTGACATACGATGTGTTCCTCAGTTTCAGAGGTAAAGATACCCGCAAAAATTTCACAGACCATCTTTATACCGCTCTCAAACAGAGAGGAATAAACACATTTAGAGATAATGAAGAACTTAAGAGTGGGGAGTCAATTGATCTAAATCTCTTGAAAGCAATTGAAGGATCAAGATATGTTATTGTGGTGTTTTCTCAAAACTACGCCGATTCTTCGTGGTGTTTGGATGAGCTTGCAAAAGTCGCTGAATGCAGGAAAGCCATGGGAAAAACAGTCCTCCCTGTGTTCTACGATGTTGATCCAAATGAGGTGAGACGACAACAGGGCAATCACTTTGAGAAAGCATTTGAGAAGCATCAAGAGCGGTTTGGAGACAATCCAGATAAATTGCAGAGGTGGAAAGACGCTCTTGCTGAAGTAGGCAATCTCTCTGGATGGCATCTGCAAGATATGTACGAATCCAAAGTTATTCAAGAAATTGTTGAAAAGATTTTCACCGAATTAAATCAAACAATCTCAATATCTGAGGGTTTAATTGGGATGGATTCTCACCTGAATGAAATGCTTTCGTACTTAGACATTGGGTGTCCCGATGTTCGTATCATAGGGATTTGTGGGATGGGCGGTATTGGTAAGACAACTATTGCACATGTGGTTTTTGAATCGGTAAAAGCTCATTTTGAAGGTTGCAGCTTTCTTGAGAATGTGAGAGAAGTAACTGAAAAGCACGGTGCAGTTCATTTACAACAGCACCTTCTTTCGAATTTGCTGAAATGTAGTGTAAATGTAGAGAACACTAAAATGGGAAAAAGTATAATGAGGCAAAGACTACGCACTAAAATGGTTCTTATCATTCTTGATGATGTGGACCAAGAAGAACAATTGGAAGCATTGTGTGATAGGGAATCGTTTGGTCCAGGGAGTAGAATCATCATAACTTCTAGAGATGAACATTTGCTCAGTGCAGTTGAAGGAGATAAAGTATATAAGGTGAATTCATTAACTGATGCTGAAGCTCTTGAGCTCTTCCGAATGAAAGCCTTTAAGAAAGACCAGCTGGTTGGGGAAGATTTTCTCAAGCTATCCAAGGAATTTTTGAAATATGCTAATGGCCTTCCACTAGCTATTACAGTTTTGGGTTCTTCTGTTAAGGGTAGAAGAGTAGAGTTATGGTTAAGTGCATTGGATAGACTAAAAAACAATCCTAAAAAAAAAATTATCAATGTTCTTAAAGTTAGTTTTGATGGATTAGAAGAAACAGAAAAAAAAATGTTTTTGGACATCGCATGTTTCTTTAAAGGAGCGTATAGAGATCATGTTACAAGAATACTACTAGGTAGTGACCACAGTCCGGACATTGATATAGAAGTTCTCATAGAGAAATCTTTGGTAACTTTGTTTGGAAGAAGATTAGGGATGCATGACTTGGTACAAGAATTGGGTTGGGAAATTGTTCGTCAAGAATGTCGTGAAGAGCCTGGAAAACGTAGTAGGTTGTGGCTTTCTGATGAAATCATTCATGTACTTGGTCGGAGTAAGGCGACGAGTGCAGTTCAAAGCATATCCCTCCAATGTCGAAGAGAAGACGATGTTGTCCACTCAAGTGATGATGCCTTTTCAAATATGGACAGATTGAGATTGCTCAAGATTTGTAATTTGAAGTTCTCTGAAAACATCAGATATCTTTCTAATGAGTTACAGTATCTTGAATGGCATAAATGTCCTTTGGATTTTTTCCCGTCAGACTTTCAACCGGATAAACTTACCGTGGTTCTTATGTTTCATAGCCGCATCAAACAACTATGGAGGGGAAAAAAAGGATGGAGCATGCTACGGGTTATTAATCTGAGTGGTTCAAAATACTTGATGTCGACTCCAGACTTCACTGAGGTTCCAGATCTTGAAGAGTTGGTGCTTCAAAATTGCACAAGCTTGGTGGAGGTTCACCCATCTCTCGGATTTCTGAAGAAACTTGGCTATTTGGATATGAAAAATTGCGAGTCTATTGAGAGCATTCCACCTTTCACTACCTTGGAATCCCTTCAAATATTGAGGCTTTCACTCTGTTCAGGACTGAAGAAGGTTCCAGAAATTGAAGGAAATATGAAAAGCTTGCTGGAGCTTTATTTGAATGGGACCAGCATTGAGGAATTACCTCCATCAATTGACCGTTTGACTGGTCTTACCACTTTGGATCTGACAGATTGTAAAAACCTTTTGCATCTTCCCAATACCATTGGGTGCTTGACATCTTTAAAAAGTCTCCGTCTAGCAGGTTGCTCCAAAATTGATGAGGTTCCTAAGAATCTGAATGGTATGAAACGTCTGGAGATGCTTACAATTGGTGGAACTTCCATAAGGGAGTTATCATTCATCGTAGGCATGAAGAATCTACAGTTACTATGCTGCGAAGGATGTAAATGCCTAGTTTCAGGATCATGTAAAGTCCTGGCTTCCTTATCGAGTTTGATAGAGCTACACTTGAATGATTGCAATCTGATGGATGGAGAAATTCTCAATGATCTTAGCAGCCTAATCTCCTTAAGAATGTTAGATTTAAGTGGTAATTTCTTTGTACGATTACCTGAAAGTATCTCTCAACTCTCAAAGCTCAATACTCTGCGCTTGACGAATTGTAGACAACTTCAATTGCTGCCCAAGAAGCTTCCGTTAAGTCTTCGAAACGTGTATGCACAAGATTGTACTTCACTGACGGATTACCCAAATCAAATCAAAGTATTGAATTCATCGGAGTCGGGAGTGACTATTGTCAACTCCCTCAATTCTTCAGCACTTGAAGTCTCAGAATCCAGAGCGGTCATTTATCCACGAAATGAGGGGGAATCTACAGAGCTGCTTTTGACACATGAGAATACAGAGGGGAAACAAGTTGATCATTATTTGCCACTGAGAAGTGTAGCGCATCAAAGAGAAGAAGTGTCTTCCTGGTCAAATTTCGCTATCGTAATTCCTACAGATGAAATTCCAGAGTGTTTCAGCACTATATCTACCAGAAATCCCATATCAATCCCGATGCCTCAAAATCTGACCGATGATAACAAGTGGAAGGGACTTGCCGCGTGTGCTATATTCTCAGTCAAGGGGCATACTGCTCTCTCTGTTATTGAACCGGATCCTGAATTTTCTAATTACTCGTATCAAATGACATTGGAAACCGATGCTGGGCGTCTGAAACCATATGTGTTGGATAGGAAAGACCTTCTTCCCCCTCTTGGATCAAGTTCTCATCTGCTTATGATTTTCTATATATCTAGTTCAAAGAAGTACTATCCACAATGGGAATTAGACATTTCAACTGCGGTGAGTGCTACGTTTGAAACCACCAATTCATCCATGGTGGTCCAGAAATGTGGAATTGGTCTAGTCTATGAGCAAGATGCTGGAATGTATTCCAGGATAGTCGATGGGGATCAACCGATTTGTCTGAATGAAGTTGAGGACGCCGCCTCTATACGGGAGTCTAGCTGGCTTAGTCAATCATTAAAATGGGAGAAATTCATTCCACCCTTGGAAGAACAATCCAGACTTGTGCTGAGAAAGAACCTTGAGTCTGTTCTTCCAAGATACCTTGAGGCGCTAAACCGTTCTTCTATAACGTACAAATTTAACCTCAGAGGAAGCCCAGGGTGGTTTCATAAGTCATTTTCCCCTGGCACGAGGTCAGATTTTATCGTAGTGTCAATCAAGCTGCCCCAAAATCTGCACACGAGCAAGAAGTGGATGGGATTTGCAATATACGCATCACTTGTAGAGGAAGTGGGGCAGCAGACAATGAAAGAGGACAACTATTGGGTATCTGTTACACTATCAACAGGGGATAAATTTGGTACGGGTGTGTCTCGAGTACTTAAGTACATCAGGCCATTGTCAGAAGAACATCATCAACTATTGGTTATTTACATACCGCGGGCAGAGATTCCCGAAGGGTTGTTCACTCAGACATTAACAACTGCAATGGACTTTTGTATCATGATCAGTGATAGCCCTCATGTAAAGGTTCAAAGATGTGGGTCACGTATTGTGTACCAGGAAGATATACAAGGTTTTTCTGATACAATTATCCGGTGCATGCAGAGGGATGATTCTCATAAATCTCACGATAAGTTGGTGGTAGAAGCATGGTTACAGTTGATACAATTGCAAGGTGCCCATATAGAGAGAATGAAAGAACGGGATTCCAGAACCCCACTGGAAAAATACTTTGAATTGCACTCACGAAAATATCGAAATTGGGGAGCTGAAATTTTCAAGTCAAAATGGTTCATGCCTTTCATCAACCAAGGCAACTCTGCAGAAATCCAATTGCCTCTGAATGTGTTCGATGATAATAATTGGTTGGGGTTTGCCGTATGTTCTGGGTTGTCCCATGACCAATATCCTAACATTAGTCTTGGCAGTACTGCCCCTGATTTAGAGAATGTTATGCATAGTCTTCGTTTGCACTCTGATGCTGCTTGGCACGGCTTATTTGATATCACCATGAAGGCATATTTTAGGATACTCGAGCCATTGGAATACGATGCAACTTTGTTCTTTTATATACCGCGCACTAATTGTCTCAGTAAGATGTGGAGGCAATGCAAATTGGCCAGGGTAACTATATGGTGCTCTAGCCCATGCGTAGTTGTGCATAGTTGTGTCCTTCGTCTAGTATTTAAGGAGGACGTTGAACATCTTGTAGAAACATTGACCCTCGCCGAATTACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1612

Amino Acids

182.76

Weight (kDa)

6.13

Isoelectric Point (pI)

44.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 39 - 210 1e-51 TIR domain
TIR_2 PF13676 41 - 143 2.8e-18 TIR domain
NB-ARC PF00931 221 - 387 9.2e-25 NB-ARC domain
NACHT PF05729 238 - 380 3.6e-06 NACHT domain
WHD_ROQ1 PF23282 454 - 523 1.8e-15 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 682 - 794 1.6e-09 Leucine-rich repeat region
LRR_13 PF23286 714 - 802 3.5e-12 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 853 - 952 6.6e-07 Leucine-rich repeat region
C-JID PF20160 1027 - 1170 8.6e-07 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1700
Acc36I ACCTGC 1 cut(s) 2372
AccB1I GGYRCC 1 cut(s) 4274
AccBSI CCGCTC 3 cut(s) 182, 472, 2924
AccI GTMKAC 2 cut(s) 1979, 2746
AccII CGCG 3 cut(s) 3188, 4036, 4675
AccIII TCCGGA 1 cut(s) 1459
AclWI GGATC 8 cut(s) 283, 401, 2310, 2551, 3236, 3249, 3352, 3546
AcoI YGGCCR 1 cut(s) 4716
AcuI CTGAAG 5 cut(s) 543, 1140, 2093, 2204, 2880
AcyI GRCGYC 2 cut(s) 3295, 3570
AfiI CCNNNNNNNGG 5 cut(s) 1459, 2063, 3291, 3341, 3582
AflII CTTAAG 3 cut(s) 227, 2650, 3977
AflIII ACRYGT 2 cut(s) 757, 2793
AjnI CCWGG 9 cut(s) 1024, 1585, 2556, 3053, 3521, 3751, 3778, 4155, 4718
AleI CACNNNNGTG 1 cut(s) 1710
Alw21I GWGCWC 2 cut(s) 1134, 4739
Alw26I GTCTC 6 cut(s) 415, 474, 2375, 2914, 3972, 4691
AlwI GGATC 8 cut(s) 283, 401, 2310, 2551, 3236, 3249, 3352, 3546
Ama87I CYCGRG 2 cut(s) 3969, 4631
Aor13HI TCCGGA 1 cut(s) 1459
AoxI GGCC 3 cut(s) 1219, 3989, 4716
ApeKI GCWGC 8 cut(s) 798, 2514, 2637, 2761, 2962, 3817, 3892, 4580
AseI ATTAAT 1 cut(s) 1950
Asp700I GAANNNNTTC 6 cut(s) 1179, 1560, 1623, 2148, 3517, 4445
AspLEI GCGC 4 cut(s) 2733, 3031, 3706, 4677
AspS9I GGNCC 4 cut(s) 976, 1022, 2244, 3469
AsuHPI GGTGA 7 cut(s) 430, 595, 650, 1111, 2045, 3442, 4597
AsuII TTCGAA 2 cut(s) 869, 2788
AvaI CYCGRG 2 cut(s) 3969, 4631
AvaII GGWCC 4 cut(s) 976, 1022, 2244, 3469
BaeGI GKGCMC 1 cut(s) 4279
BaeI ACNNNNGTAYC 4 cut(s) 3948, 3981, 4078, 4111
BalI TGGCCA 1 cut(s) 4718
BamHI GGATCC 1 cut(s) 3241
BanI GGYRCC 1 cut(s) 4274
BanII GRGCYC 1 cut(s) 1134
BarI GAAGNNNNNNTAC 2 cut(s) 4236, 4268
BauI CACGAG 2 cut(s) 3784, 3835
BbsI GAAGAC 4 cut(s) 1698, 2777, 3042, 4553
Bbv12I GWGCWC 2 cut(s) 1134, 4739
BbvI GCAGC 8 cut(s) 810, 2501, 2649, 2748, 2949, 3804, 3904, 4567
BceAI ACGGC 2 cut(s) 4460, 4606
BcgI CGANNNNNNTGC 2 cut(s) 4544, 4578
BciT130I CCWGG 9 cut(s) 1026, 1587, 2558, 3055, 3523, 3753, 3780, 4157, 4720
BciVI GTATCC 1 cut(s) 4620
BclI TGATCA 2 cut(s) 3001, 4101
BcoDI GTCTC 6 cut(s) 415, 474, 2375, 2914, 3972, 4691
BfmI CTRYAG 4 cut(s) 2501, 2955, 3081, 4419
BfoI RGCGCY 1 cut(s) 3707
BfrI CTTAAG 3 cut(s) 227, 2650, 3977
BfuAI ACCTGC 1 cut(s) 2372
BfuI GTATCC 1 cut(s) 4620
BglII AGATCT 2 cut(s) 16, 2005
BmcAI AGTACT 3 cut(s) 3395, 3975, 4528
Bme1390I CCNGG 9 cut(s) 1026, 1587, 2558, 3055, 3523, 3753, 3780, 4157, 4720
Bme18I GGWCC 4 cut(s) 976, 1022, 2244, 3469
BmeT110I CYCGRG 2 cut(s) 3969, 4631
BmgT120I GGNCC 4 cut(s) 976, 1022, 2244, 3469
BmiI GGNNCC 6 cut(s) 2001, 2193, 2245, 2406, 3243, 4276
BmrFI CCNGG 9 cut(s) 1026, 1587, 2558, 3055, 3523, 3753, 3780, 4157, 4720
BoxI GACNNNNGTC 2 cut(s) 1455, 2876
BpiI GAAGAC 4 cut(s) 1698, 2777, 3042, 4553
BplI GAGNNNNNCTC 2 cut(s) 2607, 2639
BpmI CTGGAG 3 cut(s) 1968, 2246, 2456
Bpu14I TTCGAA 2 cut(s) 869, 2788
BpuEI CTTGAG 7 cut(s) 827, 1148, 1169, 1693, 1736, 3695, 3719
BsaAI YACGTR 1 cut(s) 4145
BsaBI GATNNNNATC 4 cut(s) 252, 405, 2601, 3537
BsaHI GRCGYC 2 cut(s) 3295, 3570
BsaWI WCCGGW 4 cut(s) 1459, 1861, 3238, 4191
BsaXI ACNNNNNCTCC 2 cut(s) 1074, 1104
Bsc4I CCNNNNNNNGG 5 cut(s) 1459, 2063, 3291, 3341, 3582
Bse1I ACTGG 2 cut(s) 2290, 4323
Bse3DI GCAATG 2 cut(s) 4089, 4712
Bse8I GATNNNNATC 4 cut(s) 252, 405, 2601, 3537
BseAI TCCGGA 1 cut(s) 1459
BseBI CCWGG 9 cut(s) 1026, 1587, 2558, 3055, 3523, 3753, 3780, 4157, 4720
BseJI GATNNNNATC 4 cut(s) 252, 405, 2601, 3537
BseLI CCNNNNNNNGG 5 cut(s) 1459, 2063, 3291, 3341, 3582
BseMI GCAATG 2 cut(s) 4089, 4712
BseNI ACTGG 2 cut(s) 2290, 4323
BseRI GAGGAG 1 cut(s) 78
BseSI GKGCMC 1 cut(s) 4279
BseXI GCAGC 8 cut(s) 810, 2501, 2649, 2748, 2949, 3804, 3904, 4567
BseYI CCCAGC 1 cut(s) 3290
BsgI GTGCAG 4 cut(s) 861, 1092, 1677, 3815
Bsh1236I CGCG 3 cut(s) 3188, 4036, 4675
BshFI GGCC 3 cut(s) 1221, 3991, 4718
BshNI GGYRCC 1 cut(s) 4274
BsiHKAI GWGCWC 2 cut(s) 1134, 4739
BsiHKCI CYCGRG 2 cut(s) 3969, 4631
BsiSI CCGG 4 cut(s) 1460, 1862, 3239, 4192
BslFI GGGAC 4 cut(s) 681, 2257, 3192, 4477
BslI CCNNNNNNNGG 5 cut(s) 1459, 2063, 3291, 3341, 3582
BsmAI GTCTC 6 cut(s) 415, 474, 2375, 2914, 3972, 4691
BsmBI CGTCTC 1 cut(s) 415
BsmFI GGGAC 4 cut(s) 681, 2257, 3192, 4477
BsmI GAATGC 2 cut(s) 362, 2121
BsnI GGCC 3 cut(s) 1221, 3991, 4718
BsoBI CYCGRG 2 cut(s) 3969, 4631
Bsp119I TTCGAA 2 cut(s) 869, 2788
Bsp1286I GDGCHC 3 cut(s) 1134, 4279, 4739
Bsp13I TCCGGA 1 cut(s) 1459
Bsp19I CCATGG 2 cut(s) 369, 3462
BspANI GGCC 3 cut(s) 1221, 3991, 4718
BspEI TCCGGA 1 cut(s) 1459
BspFNI CGCG 3 cut(s) 3188, 4036, 4675
BspHI TCATGA 1 cut(s) 4098
BspLI GGNNCC 6 cut(s) 2001, 2193, 2245, 2406, 3243, 4276
BspMAI CTGCAG 1 cut(s) 4423
BspMI ACCTGC 1 cut(s) 2372
BspPI GGATC 8 cut(s) 283, 401, 2310, 2551, 3236, 3249, 3352, 3546
BspQI GCTCTTC 2 cut(s) 1139, 1575
BspT104I TTCGAA 2 cut(s) 869, 2788
BspT107I GGYRCC 1 cut(s) 4274
BspTI CTTAAG 3 cut(s) 227, 2650, 3977
BsrBI CCGCTC 3 cut(s) 182, 472, 2924
BsrDI GCAATG 2 cut(s) 4089, 4712
BsrI ACTGG 2 cut(s) 2290, 4323
BssNI GRCGYC 2 cut(s) 3295, 3570
BssSI CACGAG 2 cut(s) 3784, 3835
BssT1I CCWWGG 6 cut(s) 369, 1194, 2139, 3462, 3636, 4408
Bst2BI CACGAG 2 cut(s) 3784, 3835
Bst2UI CCWGG 9 cut(s) 1026, 1587, 2558, 3055, 3523, 3753, 3780, 4157, 4720
Bst6I CTCTTC 5 cut(s) 1139, 1261, 1575, 1681, 2007
BstACI GRCGYC 2 cut(s) 3295, 3570
BstAFI CTTAAG 3 cut(s) 227, 2650, 3977
BstBAI YACGTR 1 cut(s) 4145
BstBI TTCGAA 2 cut(s) 869, 2788
BstC8I GCNNGC 6 cut(s) 342, 1937, 2222, 3596, 4038, 4199
BstDSI CCRYGG 4 cut(s) 369, 1875, 3462, 4034
BstFNI CGCG 3 cut(s) 3188, 4036, 4675
BstH2I RGCGCY 1 cut(s) 3707
BstHHI GCGC 4 cut(s) 2733, 3031, 3706, 4677
BstMAI GTCTC 6 cut(s) 415, 474, 2375, 2914, 3972, 4691
BstMWI GCNNNNNNNGC 7 cut(s) 350, 469, 1655, 2770, 3191, 3434, 3866
BstNI CCWGG 9 cut(s) 1026, 1587, 2558, 3055, 3523, 3753, 3780, 4157, 4720
BstNSI RCATGY 4 cut(s) 761, 1398, 1939, 4201
BstPAI GACNNNNGTC 2 cut(s) 1455, 2876
BstSCI CCNGG 9 cut(s) 1024, 1585, 2556, 3053, 3521, 3751, 3778, 4155, 4718
BstSFI CTRYAG 4 cut(s) 2501, 2955, 3081, 4419
BstSLI GKGCMC 1 cut(s) 4279
BstUI CGCG 3 cut(s) 3188, 4036, 4675
BstV1I GCAGC 8 cut(s) 810, 2501, 2649, 2748, 2949, 3804, 3904, 4567
BstV2I GAAGAC 4 cut(s) 1698, 2777, 3042, 4553
BstX2I RGATCY 4 cut(s) 16, 2005, 2302, 3241
BstXI CCANNNNNNTGG 2 cut(s) 1169, 3479
BstYI RGATCY 4 cut(s) 16, 2005, 2302, 3241
BsuI GTATCC 1 cut(s) 4620
BsuRI GGCC 3 cut(s) 1221, 3991, 4718
BtgI CCRYGG 4 cut(s) 369, 1875, 3462, 4034
BtgZI GCGATG 1 cut(s) 1375
BtsIMutI CAGTG 6 cut(s) 1075, 1992, 2816, 3014, 4111, 4316
BveI ACCTGC 1 cut(s) 2372
Cac8I GCNNGC 6 cut(s) 342, 1937, 2222, 3596, 4038, 4199
CciI TCATGA 1 cut(s) 4098
CfoI GCGC 4 cut(s) 2733, 3031, 3706, 4677
Cfr13I GGNCC 4 cut(s) 976, 1022, 2244, 3469
Cfr42I CCGCGG 1 cut(s) 4037
CseI GACGC 3 cut(s) 521, 3284, 3578
CspCI CAANNNNNGTGG 2 cut(s) 2025, 2060
DraI TTTAAA 2 cut(s) 1405, 2364
DrdI GACNNNNNNGTC 1 cut(s) 1700
DseDI GACNNNNNNGTC 1 cut(s) 1700
EaeI YGGCCR 1 cut(s) 4716
Eam1104I CTCTTC 5 cut(s) 1139, 1261, 1575, 1681, 2007
EarI CTCTTC 5 cut(s) 1139, 1261, 1575, 1681, 2007
Ecl136II GAGCTC 1 cut(s) 1132
Eco130I CCWWGG 6 cut(s) 369, 1194, 2139, 3462, 3636, 4408
Eco24I GRGCYC 1 cut(s) 1134
Eco32I GATATC 2 cut(s) 1790, 4603
Eco47I GGWCC 4 cut(s) 976, 1022, 2244, 3469
Eco53kI GAGCTC 1 cut(s) 1132
Eco57I CTGAAG 5 cut(s) 543, 1140, 2093, 2204, 2880
Eco88I CYCGRG 2 cut(s) 3969, 4631
EcoICRI GAGCTC 1 cut(s) 1132
EcoRI GAATTC 2 cut(s) 1102, 2851
EcoRII CCWGG 9 cut(s) 1024, 1585, 2556, 3053, 3521, 3751, 3778, 4155, 4718
EcoRV GATATC 2 cut(s) 1790, 4603
EcoT14I CCWWGG 6 cut(s) 369, 1194, 2139, 3462, 3636, 4408
EcoT22I ATGCAT 2 cut(s) 1530, 4557
EcoT38I GRGCYC 1 cut(s) 1134
ErhI CCWWGG 6 cut(s) 369, 1194, 2139, 3462, 3636, 4408
Esp3I CGTCTC 1 cut(s) 415
FalI AAGNNNNNCTT 4 cut(s) 501, 533, 3315, 3347
FaqI GGGAC 4 cut(s) 681, 2257, 3192, 4477
FauI CCCGC 2 cut(s) 158, 4029
FauNDI CATATG 1 cut(s) 3307
FbaI TGATCA 2 cut(s) 3001, 4101
FblI GTMKAC 2 cut(s) 1979, 2746
FriOI GRGCYC 1 cut(s) 1134
GlaI GCGC 4 cut(s) 2732, 3030, 3705, 4676
GsaI CCCAGC 1 cut(s) 3294
GsuI CTGGAG 3 cut(s) 1968, 2246, 2456
HaeII RGCGCY 1 cut(s) 3707
HaeIII GGCC 3 cut(s) 1221, 3991, 4718
HapII CCGG 4 cut(s) 1460, 1862, 3239, 4192
HgaI GACGC 3 cut(s) 521, 3284, 3578
HhaI GCGC 4 cut(s) 2733, 3031, 3706, 4677
Hin1I GRCGYC 2 cut(s) 3295, 3570
Hin6I GCGC 4 cut(s) 2731, 3029, 3704, 4675
HinP1I GCGC 4 cut(s) 2731, 3029, 3704, 4675
HincII GTYRAC 2 cut(s) 1980, 2881
HindII GTYRAC 2 cut(s) 1980, 2881
HindIII AAGCTT 3 cut(s) 2038, 2218, 2771
HpaII CCGG 4 cut(s) 1460, 1862, 3239, 4192
HphI GGTGA 7 cut(s) 430, 595, 650, 1111, 2045, 3442, 4597
Hpy166II GTNNAC 8 cut(s) 976, 1705, 1980, 2053, 2747, 2881, 4062, 4153
Hpy8I GTNNAC 8 cut(s) 976, 1705, 1980, 2053, 2747, 2881, 4062, 4153
Hpy99I CGWCG 1 cut(s) 1654
HpyCH4IV ACGT 7 cut(s) 1594, 2431, 2793, 3440, 3725, 4144, 4794
HpyF10VI GCNNNNNNNGC 7 cut(s) 350, 469, 1655, 2770, 3191, 3434, 3866
HpySE526I ACGT 7 cut(s) 1594, 2431, 2793, 3440, 3725, 4144, 4794
Hsp92I GRCGYC 2 cut(s) 3295, 3570
HspAI GCGC 4 cut(s) 2731, 3029, 3704, 4675
Kpn2I TCCGGA 1 cut(s) 1459
Ksp22I TGATCA 2 cut(s) 3001, 4101
KspI CCGCGG 1 cut(s) 4037
LguI GCTCTTC 2 cut(s) 1139, 1575
LmnI GCTCC 5 cut(s) 1409, 1932, 2227, 2393, 4364
Lsp1109I GCAGC 8 cut(s) 810, 2501, 2649, 2748, 2949, 3804, 3904, 4567
MaeII ACGT 7 cut(s) 1594, 2431, 2793, 3440, 3725, 4144, 4794
MbiI CCGCTC 3 cut(s) 182, 472, 2924
MfeI CAATTG 8 cut(s) 243, 267, 989, 2271, 2448, 2756, 4265, 4430
MflI RGATCY 4 cut(s) 16, 2005, 2302, 3241
MhlI GDGCHC 3 cut(s) 1134, 4279, 4739
MlsI TGGCCA 1 cut(s) 4718
MluNI TGGCCA 1 cut(s) 4718
MlyI GAGTC 6 cut(s) 248, 1975, 2117, 2870, 3596, 3686
MmeI TCCRAC 2 cut(s) 1619, 3294
Mox20I TGGCCA 1 cut(s) 4718
Mph1103I ATGCAT 2 cut(s) 1530, 4557
MroI TCCGGA 1 cut(s) 1459
MroXI GAANNNNTTC 6 cut(s) 1179, 1560, 1623, 2148, 3517, 4445
MscI TGGCCA 1 cut(s) 4718
MslI CAYNNNNRTG 8 cut(s) 663, 908, 947, 1710, 2592, 3613, 4575, 4764
Msp20I TGGCCA 1 cut(s) 4718
MspA1I CMGCKG 2 cut(s) 1165, 4036
MspCI CTTAAG 3 cut(s) 227, 2650, 3977
MspI CCGG 4 cut(s) 1460, 1862, 3239, 4192
MspR9I CCNGG 9 cut(s) 1026, 1587, 2558, 3055, 3523, 3753, 3780, 4157, 4720
MunI CAATTG 8 cut(s) 243, 267, 989, 2271, 2448, 2756, 4265, 4430
Mva1269I GAATGC 2 cut(s) 362, 2121
MvaI CCWGG 9 cut(s) 1026, 1587, 2558, 3055, 3523, 3753, 3780, 4157, 4720
MvnI CGCG 3 cut(s) 3188, 4036, 4675
MwoI GCNNNNNNNGC 7 cut(s) 350, 469, 1655, 2770, 3191, 3434, 3866
NcoI CCATGG 2 cut(s) 369, 3462
NdeI CATATG 1 cut(s) 3307
NlaIV GGNNCC 6 cut(s) 2001, 2193, 2245, 2406, 3243, 4276
NmuCI GTSAC 3 cut(s) 1448, 2869, 4140
NsiI ATGCAT 2 cut(s) 1530, 4557
NspI RCATGY 4 cut(s) 761, 1398, 1939, 4201
NspV TTCGAA 2 cut(s) 869, 2788
OliI CACNNNNGTG 1 cut(s) 1710
PaeI GCATGC 2 cut(s) 1939, 4201
PaeR7I CTCGAG 2 cut(s) 3969, 4631
PagI TCATGA 1 cut(s) 4098
PasI CCCWGGG 1 cut(s) 3752
PciI ACATGT 1 cut(s) 757
PciSI GCTCTTC 2 cut(s) 1139, 1575
PcsI WCGNNNNNNNCGW 1 cut(s) 4351
PctI GAATGC 2 cut(s) 362, 2121
PdmI GAANNNNTTC 6 cut(s) 1179, 1560, 1623, 2148, 3517, 4445
PfoI TCCNGGA 1 cut(s) 3521
PleI GAGTC 6 cut(s) 247, 1975, 2116, 2869, 3595, 3685
PpsI GAGTC 6 cut(s) 247, 1975, 2116, 2869, 3595, 3685
Ppu21I YACGTR 1 cut(s) 4145
PscI ACATGT 1 cut(s) 757
PshAI GACNNNNGTC 2 cut(s) 1455, 2876
PshBI ATTAAT 1 cut(s) 1950
Psp124BI GAGCTC 1 cut(s) 1134
Psp6I CCWGG 9 cut(s) 1024, 1585, 2556, 3053, 3521, 3751, 3778, 4155, 4718
PspFI CCCAGC 1 cut(s) 3290
PspGI CCWGG 9 cut(s) 1024, 1585, 2556, 3053, 3521, 3751, 3778, 4155, 4718
PspN4I GGNNCC 6 cut(s) 2001, 2193, 2245, 2406, 3243, 4276
PspPI GGNCC 4 cut(s) 976, 1022, 2244, 3469
PspXI VCTCGAGB 1 cut(s) 4631
PstI CTGCAG 1 cut(s) 4423
PsuI RGATCY 4 cut(s) 16, 2005, 2302, 3241
PvuII CAGCTG 1 cut(s) 1165
RseI CAYNNNNRTG 8 cut(s) 663, 908, 947, 1710, 2592, 3613, 4575, 4764
SacI GAGCTC 1 cut(s) 1134
SacII CCGCGG 1 cut(s) 4037
SalI GTCGAC 1 cut(s) 1978
SapI GCTCTTC 2 cut(s) 1139, 1575
Sau96I GGNCC 4 cut(s) 976, 1022, 2244, 3469
ScaI AGTACT 3 cut(s) 3395, 3975, 4528
SchI GAGTC 6 cut(s) 248, 1975, 2117, 2870, 3596, 3686
ScrFI CCNGG 9 cut(s) 1026, 1587, 2558, 3055, 3523, 3753, 3780, 4157, 4720
SduI GDGCHC 3 cut(s) 1134, 4279, 4739
SfcI CTRYAG 4 cut(s) 2501, 2955, 3081, 4419
Sfr274I CTCGAG 2 cut(s) 3969, 4631
Sfr303I CCGCGG 1 cut(s) 4037
SfuI TTCGAA 2 cut(s) 869, 2788
SgrBI CCGCGG 1 cut(s) 4037
SinI GGWCC 4 cut(s) 976, 1022, 2244, 3469
SlaI CTCGAG 2 cut(s) 3969, 4631
SmiMI CAYNNNNRTG 8 cut(s) 663, 908, 947, 1710, 2592, 3613, 4575, 4764
SphI GCATGC 2 cut(s) 1939, 4201
SspI AATATT 1 cut(s) 2157
SstI GAGCTC 1 cut(s) 1134
StyD4I CCNGG 9 cut(s) 1024, 1585, 2556, 3053, 3521, 3751, 3778, 4155, 4718
StyI CCWWGG 6 cut(s) 369, 1194, 2139, 3462, 3636, 4408
TaiI ACGT 7 cut(s) 1597, 2434, 2796, 3443, 3728, 4147, 4797
TaqII GACCGA 1 cut(s) 3173
TatI WGTACW 6 cut(s) 1630, 2810, 3393, 3973, 3981, 4526
TauI GCSGC 3 cut(s) 1899, 3188, 3575
TscAI CASTG 6 cut(s) 1075, 1999, 2823, 3021, 4111, 4323
TseFI GTSAC 3 cut(s) 1448, 2869, 4140
TseI GCWGC 8 cut(s) 798, 2514, 2637, 2761, 2962, 3817, 3892, 4580
Tsp45I GTSAC 3 cut(s) 1448, 2869, 4140
TspGWI ACGGA 3 cut(s) 2363, 2766, 2837
TspRI CASTG 6 cut(s) 1075, 1999, 2823, 3021, 4111, 4323
Vha464I CTTAAG 3 cut(s) 227, 2650, 3977
VpaK11BI GGWCC 4 cut(s) 976, 1022, 2244, 3469
VspI ATTAAT 1 cut(s) 1950
XbaI TCTAGA 1 cut(s) 1049
XceI RCATGY 4 cut(s) 761, 1398, 1939, 4201
XcmI CCANNNNNNNNNTGG 2 cut(s) 3460, 4315
XhoI CTCGAG 2 cut(s) 3969, 4631
XmiI GTMKAC 2 cut(s) 1979, 2746
XmnI GAANNNNTTC 6 cut(s) 1179, 1560, 1623, 2148, 3517, 4445
ZrmI AGTACT 3 cut(s) 3395, 3975, 4528
Zsp2I ATGCAT 2 cut(s) 1530, 4557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.