Rh5BG508500

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000129
Physical Location & Seq
Forward (+)
80803876 .. 80828985
25110 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG508500.1

Sequence Viewer

Length: 4713 bp
ATGAACCCACCAACCCCGCCGCCGCCGCCAGCAACGTCCGGAGGCCAGGATCCACCCCCTCCCCCTCTCAAACTTGGAAAAGAATCCGTGAGACCCGGACCAAAGAGCTTCAAAACTGATGTTATTCCTCCTTCTTCCCATACTCCCGCAACTCGATGGAAATATGATGTCTTCCTCAGTTTCAGAGGTGAAGACACCCGGAAGAATTTTACAGAGTGTCTGTATCGTGCTTTGGTTAAGAGACGAATATCGACATTTCGAGATGATGAAGAACTTCGGAGGGGGAACGACATCTTCCCAGGACTGTTGAAAGCAGTTGAAGAATCACAGATTGTAATTGTCATCCTTTCAACCAACTATGCCAATTCAACTTGGTGTTTGGATGAGCTTGCAAAGGCCGTTGAATGCCGGAAAGTGCTGGGGCATAAAGTATTCCCAGTTTTCTATGATGTATATCCTTATGAGGTGCGAACACAAACGGGCTATTTTGGGAAAGCTTTCTCCCAGCATGAACAACACTTCAAAGACGATAGGGAAAAGATCGGAAAGTGGAGAGCTGCACTTACAGAGGTAGCCAATCTCTCTGGGTGGGATTTACGACATGGAAATGACGCCAAAGGTATTCAAGATATTGTTGAAAGGGTTTTCATTGAATTAAATTCAACGGTCTTAAGCCAAGCCAAGGGTTTGGTTGGGATGGAATATCGTGTAGAACAAGTGCTTTCGTGCTTAGGTATGAGCAGGTTTCTTGGTGTTCGTGTCATAGGGATTTGCGGATTGGGGGGAATTGGTAAGACAACTATTGCACAAGTGGTTTTTGAAAGAATATCTTCTCAGTTTGATGCTTGCAGCTTTCTTGCCAATGTTAGAGAGGTATCTGAGGGACAAGGTCTAGTTAATTTACAACAGAAACTTCTTTCGGACACCATGCCGCAAACTAATGCAAATATACGTGATATTGATATGGGGATCAAGAAAATAAAGCAGCGTCTATGTGCAAAAAAGGTTCTTGTCATTGTTGATGACGTGGATCGGCTGGACACATTGAAAGCCTTGTGCGGTAGTCATGACTGGTTCGGTCCAGGGAGTAGAATAATCGTAACATCAAGGGATAGAAGTTTGCTGTGCGCATATAAACCAGATGAAATGTATGAGGTTGAGAGATTAAATGAAGCTGAAGCTTTTCAGCTCTTTGGTTGGAGATGCTTCAAGAAATACCCAATTGAAGAAGATTTTCTGCAGCTATCAGAGCATGTTTTAAAGCATGCTAATGGCCTTCCATTGGCTATTAAAGTTTTGGGTTCCTTCCTCTACGATAAGAACGTGGAAGAATGGTCAACTGCATTGAAAGGACTAGAAAAAAATTCTGAGGAATTTTTTAAAGTTTGTTATGATGGATTATCAGAAACAGATAAAGATGTATTTTTGGACATTGCATGTTTCCATAAGGGTGAGGACGTAGATCGTGTAACAAGAATACTAGAGAGTTCTATCGGAGTCCTCTTGGATAAATCCCTAGTAAATCTAATTTCCAGCAGAAAGTTGTGGATGCCTGATTTCATTCAAGAAATGGGTCGGAAAATTGTTAGACATGAATGTCTTGAAGATCCCGGAAAACGTAGTAGGTTGTGGCTTCCTGATGATATCATCCCTGTACTTACAACTAATAAGGGAACAGCTACAGTCGAAGGTATATTCCTGAATTTTCCAGAACAAACAGAGGTTGAGTTGACTATTGATCCCTTCTTAAAGATGCGAAACCTTAAATATCTTAAAGTTTGTAATGTGAACTTTTCTGGAAACCTTGAATACCTTTGTGATGAGTTGCGACTTTTGGATTGGCATGCATGTCCTTTGAAATATCTGCCATCAGACTATCAGCCGGATAAGCTTGTTGAACTCGTCATGCAATCTAGCAATATCAAACAATTGTGGAATGGAGTTAAACGCTGCAGCATGCTAAAGCTCATTGATCTGAGCGATTCCCAATACTTGATCGAGACACCAGACTTCACCGAAGTTCCAAATATTGAGACTCTGGTGCTTGAAGGATGTACAAGTTTGGCTAAGGTCCACCCATCCATTGGATGTCTCAAAAAACTTGCCTCGTTGAATATGAGAAACTGCATATCTGTTCGGAGCCTTCTGCCTTCCATTGGTTTGGAATCTCTTGAAGTTTTTAATCTCTCTGGTTGTTCAAGACTGACGAAGTTTCCCGAAATTGAAGGAAATATGGAAAAGTTATTGGAGCTTCATCTAGATGGGACTGCTATAGTGGAATTACCTCAATCAATAAAACGTTTGACTGGTCTGTCTTTGTTGAATTTAGAAGACTGCAAAAACCTTCAGCATCTTCCAAGAGGCATCAGAAGCTCTATTCCATCTGTAAAAATCATTCTAACAGGTTGCTCTAATTTTGTTATACCTGATGATCTGGGTCCTGTGGAACGTTTGGAGGAAATAAGTGGAACTGCTAAGGGGAATTTAAAGTCTGAAGAACGTATGTCCCCTCCACCTCTACTACCACGGCGTCAGCGCTTCTGGAATAGGGTCTTGTCTTGGTTTTATCCAAACTTACGTGATTCAAATCTGATAGAGAGAGTAATTCCCAATGATGGCCGTCTATGCTCTGAAGTCTTAACTTTAATTTATAACAGTGAGTCAATTGGCACTCCCATCGGCATTCGATTCCTCGAGTTTCTTAGTTGGTTGTTGCATTCACTGTTAGATTGCCAAGATCAATCAAAGGAATGCACTTCAGGTATCTCAGCAACGGCAAGTTGCCATTCCCTCATTAAGTCACTTCCTGTCACTCATCATATAGAGAAAATTGTGGAACCCGACGAAGAAAGGCGAGCAAAAGAAACAGAGCGGGAATCTTCGGAGCTGTACTCAACCATTGTACCAGTGCAGAGGGCACAAAAAGGGAAACAAAAGCATATGAGCATCTCTCAACATCCGTTAAATAGTCACACAGGAAAAGAAGTAGCAGATACAGTGCTGTGCCCTCCCACTGAACACAAGCAAATGGCACAAGAAAAGGAAGAAACAGGAATGAGCGGTGTGAATTTGGAAGAAAACATGGGGCTTGCAGATGCAGTCAAGGTTGCTGTTGAGAAAGACTTAATTCAATATTGTTGCCTCTTTCCATCTGGTTATGAATTTAGAGAAGATGAGTTGGTTCAACTATGGATAGCTGAAGGCCTTATTAAGGAAGAGAGCAATGAAAAGAGAATGGAGGATACTGGGATTGACCATTTCAATTCATTGGTGCGTAAAGGTTTTCTAGTGCGCTCAAGATGTGATTTTAAAGTGGATTTTGATTCGGTGATCTCAGTGCCCATGTATAGTCCCAGCACCTTTTTCTACGAAGTAAATCCGGTTGAGCTTTCACCGTCTGCAAACAAAAGTTCTCCTGCTAGATATTGCAGAGCCATAGATGGTAAGTTTGATGGAGCCACCGAATCAGTGACTCATTTGTCTTTGGTTGATGAGGACATTGATGATATGACTTTTGGGGTTCTCCAAAAATTCAAGAATCTGCGCACACTGCTTTTGCTCTCTGGTCGTGGATCCTCCATCAAACGTATTCCTGGTGAGTTGTTTTCGAGCTTAAACTTCTTGACAACATTGAATTTGAGTCGAACACTCATCTCTGGATTGCCAAGTTCCGTTGGAGATGTAAAGCCGTTACGATATCTTGATGTCTCCTATACACTGATTACACGATTGCCTGAATCAATTGGTACTCTTGAAAACTTAGAGACCTTAAAACTCAGAGGTTGTAAAGATTTGTTTCATTTACCAAGGAGTGTGCACAACCTCACTGCTCTACGACATCTCGATTTTGATATCATTAGACAACTGGATATCATGCCTGCTCAGTTGGGAAAATTGAGTAACCTACAGAGTTTGTCTGCATTTCTTGTGGGTAGAGATGATGGGCGCCGTATCCGAGAGCTGAAGAATTTAAATGATCTTGGAGGAGCACTTCGCATTTTTAGGCTTGAAAATGTGTCGAGTCGGGCAGATGCTGTGGAAGTGGATTTGGAAAACAAGAAAAGACTGCAGAGATTAGATTTCCGATGGACTAAACTAATGGGTGAGAACGAAAAGAAGATGCCAGAAGAGATCCTCGACTCTCTGCGACCCCCTACGGGTCTTAAAGAGTTGCAGATATTGTTCTACAGTGGAACAAAATTCCCATCTTGGTTGAGCGATACATCTATTACTGACCTTGTTGTTATCACTCTCTACAAATGTGGAAACTGTGAGCATCTCCCATCTCTCGGTCGATTACCAAACCTTAAGTTTCTTTCAATTGTAGAGATGAATGAGGTGAAGGAAATCGCTCCGACATTTTTCAGAAGTCTTGATCACCCAAAATCCTTGAAGGCGTTTCCAAAACTTGAGATACTAGAGGTCGACAGCATGCTCAATTTGAAAGACTGGAAGGAAGTACAAGAAGGTGACTTGGATTCCCTCATTAAACTCACAATGGAGTCTTGCCCTGAACTTGTTACTGTTCCATCATTTTCAGGCCTCAAAACCCTCAAGTATTTAGAGTTTAACCACTGCCCAGAGCTTCTTTCATTGGACTGCAATGCAGTACCAACTTCACTCGAGTCTTTAATAATACGAGACTGCCCGAAACTGAAAGAATGGAGCCACGAGGGACATCAAGATTTGAGTTTCCTCAAGTCACGTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

1570

Amino Acids

177.63

Weight (kDa)

6.0

Isoelectric Point (pI)

47.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 54 - 231 2e-49 TIR domain
TIR_2 PF13676 57 - 153 2.6e-14 TIR domain
NB-ARC PF00931 234 - 403 9.9e-18 NB-ARC domain
NACHT PF05729 254 - 398 3.4e-06 NACHT domain
WHD_ROQ1 PF23282 469 - 531 2e-11 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 721 - 805 2.1e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
WHD_DRP PF23559 1048 - 1097 4.4e-14 Disease resistance protein Winged helix domain
LRR_14 PF23598 1230 - 1476 5.4e-27 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 1318 - 1444 3.3e-39 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2652
AasI GACNNNNNNGTC 3 cut(s) 1596, 2311, 3478
Acc16I TGCGCA 2 cut(s) 1129, 3545
Acc36I ACCTGC 1 cut(s) 732
AccB1I GGYRCC 1 cut(s) 3945
AccB7I CCANNNNNTGG 1 cut(s) 2084
AccBSI CCGCTC 2 cut(s) 2872, 3060
AccI GTMKAC 1 cut(s) 4425
AccIII TCCGGA 1 cut(s) 38
AclI AACGTT 2 cut(s) 2299, 2449
AclWI GGATC 9 cut(s) 44, 57, 977, 1038, 1601, 1733, 3567, 3580, 4126
AcoI YGGCCR 1 cut(s) 2617
AcuI CTGAAG 7 cut(s) 1197, 2330, 2514, 2652, 2742, 3219, 3983
AcyI GRCGYC 3 cut(s) 612, 2530, 3946
AfaI GTAC 7 cut(s) 1656, 2056, 2891, 2904, 3748, 4461, 4611
AfeI AGCGCT 1 cut(s) 2537
AfiI CCNNNNNNNGG 8 cut(s) 682, 1282, 2084, 2156, 2615, 3211, 4157, 4289
AflII CTTAAG 2 cut(s) 670, 4307
AjiI CACGTC 1 cut(s) 1027
AjnI CCWGG 4 cut(s) 45, 298, 1081, 3592
Alw21I GWGCWC 2 cut(s) 3819, 3991
Alw26I GTCTC 8 cut(s) 85, 235, 1994, 2027, 2096, 3712, 3758, 4635
Alw44I GTGCAC 1 cut(s) 3815
AlwI GGATC 9 cut(s) 44, 57, 977, 1038, 1601, 1733, 3567, 3580, 4126
AlwNI CAGNNNCTG 1 cut(s) 4034
Ama87I CYCGRG 2 cut(s) 2693, 4622
Aor13HI TCCGGA 1 cut(s) 38
Aor51HI AGCGCT 1 cut(s) 2537
AoxI GGCC 6 cut(s) 43, 396, 1273, 2617, 3202, 4540
ApaLI GTGCAC 1 cut(s) 3815
ApeKI GCWGC 6 cut(s) 557, 849, 985, 1240, 1950, 1953
Asp700I GAANNNNTTC 5 cut(s) 273, 497, 829, 1182, 1233
AspLEI GCGC 5 cut(s) 1130, 2538, 3294, 3546, 3948
AspS9I GGNCC 4 cut(s) 98, 1079, 2071, 2438
AsuC2I CCSGG 3 cut(s) 96, 199, 1611
AvaI CYCGRG 2 cut(s) 2693, 4622
AvaII GGWCC 4 cut(s) 98, 1079, 2071, 2438
BaeGI GKGCMC 4 cut(s) 2920, 3008, 3342, 3819
BaeI ACNNNNGTAYC 2 cut(s) 2886, 2919
BamHI GGATCC 2 cut(s) 49, 3572
BanI GGYRCC 1 cut(s) 3945
BauI CACGAG 1 cut(s) 4670
BbsI GAAGAC 3 cut(s) 163, 198, 2337
Bbv12I GWGCWC 2 cut(s) 3819, 3991
BbvI GCAGC 6 cut(s) 544, 861, 997, 1252, 1937, 1965
BceAI ACGGC 6 cut(s) 383, 2543, 2604, 2790, 3673, 3933
BcgI CGANNNNNNTGC 2 cut(s) 2659, 2693
BciT130I CCWGG 4 cut(s) 47, 300, 1083, 3594
BciVI GTATCC 2 cut(s) 3235, 3962
BclI TGATCA 1 cut(s) 4375
BcnI CCSGG 3 cut(s) 96, 199, 1611
BcoDI GTCTC 8 cut(s) 85, 235, 1994, 2027, 2096, 3712, 3758, 4635
BfaI CTAG 9 cut(s) 893, 1355, 1481, 1517, 1914, 2258, 3287, 3420, 4418
BfmI CTRYAG 7 cut(s) 1238, 1680, 1951, 2271, 3905, 4067, 4186
BfoI RGCGCY 2 cut(s) 2539, 3949
BfrI CTTAAG 2 cut(s) 670, 4307
BfuAI ACCTGC 1 cut(s) 732
BfuI GTATCC 2 cut(s) 3235, 3962
Bme1390I CCNGG 7 cut(s) 47, 96, 199, 300, 1083, 1611, 3594
Bme18I GGWCC 4 cut(s) 98, 1079, 2071, 2438
BmeT110I CYCGRG 2 cut(s) 2693, 4622
BmgBI CACGTC 1 cut(s) 1027
BmgT120I GGNCC 4 cut(s) 98, 1079, 2071, 2438
BmiI GGNNCC 9 cut(s) 51, 1303, 2141, 2439, 2838, 3457, 3574, 3947, 4667
BmrFI CCNGG 7 cut(s) 47, 96, 199, 300, 1083, 1611, 3594
BmrI ACTGGG 2 cut(s) 431, 3255
BmuI ACTGGG 2 cut(s) 431, 3255
BpiI GAAGAC 3 cut(s) 163, 198, 2337
BplI GAGNNNNNCTC 4 cut(s) 2876, 2908, 2935, 2967
Bpu10I CCTNAGC 3 cut(s) 730, 2067, 2475
BpuEI CTTGAG 4 cut(s) 3280, 4430, 4538, 4682
BpuMI CCSGG 3 cut(s) 96, 199, 1611
BsaAI YACGTR 2 cut(s) 953, 2579
BsaBI GATNNNNATC 2 cut(s) 453, 2586
BsaHI GRCGYC 3 cut(s) 612, 2530, 3946
BsaI GGTCTC 2 cut(s) 85, 3758
BsaJI CCNNGG 5 cut(s) 298, 681, 1082, 2525, 3806
BsaWI WCCGGW 2 cut(s) 38, 3379
Bsc4I CCNNNNNNNGG 8 cut(s) 682, 1282, 2084, 2156, 2615, 3211, 4157, 4289
Bse1I ACTGG 7 cut(s) 437, 1076, 2311, 2906, 3250, 3870, 4454
Bse3DI GCAATG 3 cut(s) 1431, 3229, 4609
Bse8I GATNNNNATC 2 cut(s) 453, 2586
BseAI TCCGGA 1 cut(s) 38
BseBI CCWGG 4 cut(s) 47, 300, 1083, 3594
BseDI CCNNGG 5 cut(s) 298, 681, 1082, 2525, 3806
BseGI GGATG 9 cut(s) 342, 388, 702, 1554, 1647, 2057, 2078, 2093, 2956
BseJI GATNNNNATC 2 cut(s) 453, 2586
BseLI CCNNNNNNNGG 8 cut(s) 682, 1282, 2084, 2156, 2615, 3211, 4157, 4289
BseMI GCAATG 3 cut(s) 1431, 3229, 4609
BseMII CTCAG 9 cut(s) 190, 848, 870, 1359, 1967, 2781, 3348, 3790, 3896
BseNI ACTGG 7 cut(s) 437, 1076, 2311, 2906, 3250, 3870, 4454
BseRI GAGGAG 1 cut(s) 3999
BseSI GKGCMC 4 cut(s) 2920, 3008, 3342, 3819
BseXI GCAGC 6 cut(s) 544, 861, 997, 1252, 1937, 1965
BseYI CCCAGC 3 cut(s) 418, 504, 3353
BsgI GTGCAG 2 cut(s) 543, 2930
Bsh1285I CGRYCG 1 cut(s) 4294
BshFI GGCC 6 cut(s) 45, 398, 1275, 2619, 3204, 4542
BshNI GGYRCC 1 cut(s) 3945
BsiEI CGRYCG 1 cut(s) 4294
BsiHKAI GWGCWC 2 cut(s) 3819, 3991
BsiHKCI CYCGRG 2 cut(s) 2693, 4622
BsiSI CCGG 7 cut(s) 39, 96, 199, 409, 1611, 1883, 3380
BslFI GGGAC 5 cut(s) 897, 2278, 2491, 3336, 4689
BslI CCNNNNNNNGG 8 cut(s) 682, 1282, 2084, 2156, 2615, 3211, 4157, 4289
BsmAI GTCTC 8 cut(s) 85, 235, 1994, 2027, 2096, 3712, 3758, 4635
BsmBI CGTCTC 1 cut(s) 235
BsmFI GGGAC 5 cut(s) 897, 2278, 2491, 3336, 4689
BsmI GAATGC 4 cut(s) 410, 2682, 2716, 2756
BsnI GGCC 6 cut(s) 45, 398, 1275, 2619, 3204, 4542
Bso31I GGTCTC 2 cut(s) 85, 3758
BsoBI CYCGRG 2 cut(s) 2693, 4622
Bsp1286I GDGCHC 5 cut(s) 2920, 3008, 3342, 3819, 3991
Bsp13I TCCGGA 1 cut(s) 38
Bsp1407I TGTACA 1 cut(s) 2054
BspANI GGCC 6 cut(s) 45, 398, 1275, 2619, 3204, 4542
BspCNI CTCAG 9 cut(s) 189, 847, 871, 1360, 1968, 2780, 3347, 3789, 3895
BspEI TCCGGA 1 cut(s) 38
BspHI TCATGA 1 cut(s) 1066
BspLI GGNNCC 9 cut(s) 51, 1303, 2141, 2439, 2838, 3457, 3574, 3947, 4667
BspMAI CTGCAG 3 cut(s) 1242, 1955, 4071
BspMI ACCTGC 1 cut(s) 732
BspPI GGATC 9 cut(s) 44, 57, 977, 1038, 1601, 1733, 3567, 3580, 4126
BspT107I GGYRCC 1 cut(s) 3945
BspTI CTTAAG 2 cut(s) 670, 4307
BspTNI GGTCTC 2 cut(s) 85, 3758
BsrBI CCGCTC 2 cut(s) 2872, 3060
BsrDI GCAATG 3 cut(s) 1431, 3229, 4609
BsrGI TGTACA 1 cut(s) 2054
BsrI ACTGG 7 cut(s) 437, 1076, 2311, 2906, 3250, 3870, 4454
BssECI CCNNGG 5 cut(s) 298, 681, 1082, 2525, 3806
BssNI GRCGYC 3 cut(s) 612, 2530, 3946
BssSI CACGAG 1 cut(s) 4670
BssT1I CCWWGG 2 cut(s) 681, 3806
Bst2BI CACGAG 1 cut(s) 4670
Bst2UI CCWGG 4 cut(s) 47, 300, 1083, 3594
Bst6I CTCTTC 2 cut(s) 3210, 4122
BstACI GRCGYC 3 cut(s) 612, 2530, 3946
BstAFI CTTAAG 2 cut(s) 670, 4307
BstAUI TGTACA 1 cut(s) 2054
BstBAI YACGTR 2 cut(s) 953, 2579
BstDSI CCRYGG 1 cut(s) 2525
BstENI CCTNNNNNAGG 1 cut(s) 3209
BstF5I GGATG 9 cut(s) 342, 388, 702, 1554, 1647, 2057, 2078, 2093, 2956
BstH2I RGCGCY 2 cut(s) 2539, 3949
BstHHI GCGC 5 cut(s) 1130, 2538, 3294, 3546, 3948
BstMAI GTCTC 8 cut(s) 85, 235, 1994, 2027, 2096, 3712, 3758, 4635
BstMCI CGRYCG 1 cut(s) 4294
BstMWI GCNNNNNNNGC 6 cut(s) 25, 1249, 1888, 2370, 2625, 3550
BstNI CCWGG 4 cut(s) 47, 300, 1083, 3594
BstNSI RCATGY 7 cut(s) 1256, 1268, 1440, 1847, 1851, 1960, 4435
BstSCI CCNGG 7 cut(s) 45, 94, 197, 298, 1081, 1609, 3592
BstSFI CTRYAG 7 cut(s) 1238, 1680, 1951, 2271, 3905, 4067, 4186
BstSLI GKGCMC 4 cut(s) 2920, 3008, 3342, 3819
BstV1I GCAGC 6 cut(s) 544, 861, 997, 1252, 1937, 1965
BstV2I GAAGAC 3 cut(s) 163, 198, 2337
BstX2I RGATCY 4 cut(s) 49, 1606, 3572, 4131
BstXI CCANNNNNNTGG 2 cut(s) 688, 2161
BstYI RGATCY 4 cut(s) 49, 1606, 3572, 4131
BsuI GTATCC 2 cut(s) 3235, 3962
BsuRI GGCC 6 cut(s) 45, 398, 1275, 2619, 3204, 4542
BtgI CCRYGG 1 cut(s) 2525
BtrI CACGTC 1 cut(s) 1027
BtsCI GGATG 9 cut(s) 342, 388, 702, 1554, 1647, 2057, 2078, 2093, 2956
BtsI GCAGTG 3 cut(s) 3548, 3825, 4573
BveI ACCTGC 1 cut(s) 732
CaiI CAGNNNCTG 1 cut(s) 4034
CciI TCATGA 1 cut(s) 1066
CfoI GCGC 5 cut(s) 1130, 2538, 3294, 3546, 3948
Cfr13I GGNCC 4 cut(s) 98, 1079, 2071, 2438
CseI GACGC 3 cut(s) 620, 977, 2519
Csp6I GTAC 7 cut(s) 1655, 2055, 2890, 2903, 3747, 4460, 4610
CviQI GTAC 7 cut(s) 1655, 2055, 2890, 2903, 3747, 4460, 4610
DinI GGCGCC 1 cut(s) 3947
DraI TTTAAA 5 cut(s) 1260, 1381, 2487, 3310, 3972
DrdI GACNNNNNNGTC 3 cut(s) 1596, 2311, 3478
DseDI GACNNNNNNGTC 3 cut(s) 1596, 2311, 3478
EaeI YGGCCR 1 cut(s) 2617
Eam1104I CTCTTC 2 cut(s) 3210, 4122
EarI CTCTTC 2 cut(s) 3210, 4122
Eco130I CCWWGG 2 cut(s) 681, 3806
Eco147I AGGCCT 2 cut(s) 3204, 4542
Eco31I GGTCTC 2 cut(s) 85, 3758
Eco32I GATATC 4 cut(s) 1645, 3698, 3853, 3871
Eco47I GGWCC 4 cut(s) 98, 1079, 2071, 2438
Eco47III AGCGCT 1 cut(s) 2537
Eco57I CTGAAG 7 cut(s) 1197, 2330, 2514, 2652, 2742, 3219, 3983
Eco88I CYCGRG 2 cut(s) 2693, 4622
EcoNI CCTNNNNNAGG 1 cut(s) 3209
EcoO109I RGGNCCY 1 cut(s) 2438
EcoRII CCWGG 4 cut(s) 45, 298, 1081, 3592
EcoRV GATATC 4 cut(s) 1645, 3698, 3853, 3871
EcoT14I CCWWGG 2 cut(s) 681, 3806
EcoT22I ATGCAT 1 cut(s) 1849
EgeI GGCGCC 1 cut(s) 3947
EheI GGCGCC 1 cut(s) 3947
ErhI CCWWGG 2 cut(s) 681, 3806
Esp3I CGTCTC 1 cut(s) 235
FalI AAGNNNNNCTT 2 cut(s) 814, 846
FaqI GGGAC 5 cut(s) 897, 2278, 2491, 3336, 4689
FauI CCCGC 3 cut(s) 24, 154, 2865
FauNDI CATATG 1 cut(s) 2940
FbaI TGATCA 1 cut(s) 4375
FblI GTMKAC 1 cut(s) 4425
FokI GGATG 9 cut(s) 329, 395, 709, 1561, 1634, 2064, 2065, 2100, 2943
FspAI RTGCGCAY 1 cut(s) 1129
FspBI CTAG 9 cut(s) 893, 1355, 1481, 1517, 1914, 2258, 3287, 3420, 4418
FspI TGCGCA 2 cut(s) 1129, 3545
GlaI GCGC 5 cut(s) 1129, 2537, 3293, 3545, 3947
GsaI CCCAGC 3 cut(s) 422, 508, 3357
HaeII RGCGCY 2 cut(s) 2539, 3949
HaeIII GGCC 6 cut(s) 45, 398, 1275, 2619, 3204, 4542
HapII CCGG 7 cut(s) 39, 96, 199, 409, 1611, 1883, 3380
HgaI GACGC 3 cut(s) 620, 977, 2519
HhaI GCGC 5 cut(s) 1130, 2538, 3294, 3546, 3948
Hin1I GRCGYC 3 cut(s) 612, 2530, 3946
Hin6I GCGC 5 cut(s) 1128, 2536, 3292, 3544, 3946
HinP1I GCGC 5 cut(s) 1128, 2536, 3292, 3544, 3946
HincII GTYRAC 3 cut(s) 1338, 1731, 4426
HindII GTYRAC 3 cut(s) 1338, 1731, 4426
HindIII AAGCTT 3 cut(s) 495, 1179, 1889
HpaII CCGG 7 cut(s) 39, 96, 199, 409, 1611, 1883, 3380
Hpy166II GTNNAC 6 cut(s) 1338, 1731, 1789, 2074, 3817, 4426
Hpy8I GTNNAC 6 cut(s) 1338, 1731, 1789, 2074, 3817, 4426
Hpy99I CGWCG 1 cut(s) 2846
HpyF10VI GCNNNNNNNGC 6 cut(s) 25, 1249, 1888, 2370, 2625, 3550
Hsp92I GRCGYC 3 cut(s) 612, 2530, 3946
HspAI GCGC 5 cut(s) 1128, 2536, 3292, 3544, 3946
KasI GGCGCC 1 cut(s) 3945
Kpn2I TCCGGA 1 cut(s) 38
Ksp22I TGATCA 1 cut(s) 4375
LmnI GCTCC 7 cut(s) 2139, 2248, 2884, 3455, 3986, 4357, 4665
Lsp1109I GCAGC 6 cut(s) 544, 861, 997, 1252, 1937, 1965
MaeI CTAG 9 cut(s) 893, 1355, 1481, 1517, 1914, 2258, 3287, 3420, 4418
MbiI CCGCTC 2 cut(s) 2872, 3060
MfeI CAATTG 5 cut(s) 1221, 1928, 2664, 3741, 4320
MflI RGATCY 4 cut(s) 49, 1606, 3572, 4131
MhlI GDGCHC 5 cut(s) 2920, 3008, 3342, 3819, 3991
Mly113I GGCGCC 1 cut(s) 3946
MlyI GAGTC 9 cut(s) 1506, 2029, 2669, 3466, 3649, 4030, 4133, 4511, 4634
MmeI TCCRAC 4 cut(s) 1178, 1556, 3655, 4379
Mph1103I ATGCAT 1 cut(s) 1849
MroI TCCGGA 1 cut(s) 38
MroXI GAANNNNTTC 5 cut(s) 273, 497, 829, 1182, 1233
MslI CAYNNNNRTG 4 cut(s) 606, 1269, 1449, 2259
MspCI CTTAAG 2 cut(s) 670, 4307
MspI CCGG 7 cut(s) 39, 96, 199, 409, 1611, 1883, 3380
MspR9I CCNGG 7 cut(s) 47, 96, 199, 300, 1083, 1611, 3594
MunI CAATTG 5 cut(s) 1221, 1928, 2664, 3741, 4320
Mva1269I GAATGC 4 cut(s) 410, 2682, 2716, 2756
MvaI CCWGG 4 cut(s) 47, 300, 1083, 3594
MwoI GCNNNNNNNGC 6 cut(s) 25, 1249, 1888, 2370, 2625, 3550
NarI GGCGCC 1 cut(s) 3946
NciI CCSGG 3 cut(s) 96, 199, 1611
NdeI CATATG 1 cut(s) 2940
NlaIV GGNNCC 9 cut(s) 51, 1303, 2141, 2439, 2838, 3457, 3574, 3947, 4667
NmuCI GTSAC 6 cut(s) 2799, 2809, 2969, 3469, 4469, 4701
NsbI TGCGCA 2 cut(s) 1129, 3545
NsiI ATGCAT 1 cut(s) 1849
NspI RCATGY 7 cut(s) 1256, 1268, 1440, 1847, 1851, 1960, 4435
PaeI GCATGC 4 cut(s) 1268, 1847, 1960, 4435
PaeR7I CTCGAG 2 cut(s) 2693, 4622
PagI TCATGA 1 cut(s) 1066
PceI AGGCCT 2 cut(s) 3204, 4542
PcsI WCGNNNNNNNCGW 1 cut(s) 1320
PctI GAATGC 4 cut(s) 410, 2682, 2716, 2756
PdmI GAANNNNTTC 5 cut(s) 273, 497, 829, 1182, 1233
PflFI GACNNNGTC 1 cut(s) 888
PflMI CCANNNNNTGG 1 cut(s) 2084
PfoI TCCNGGA 1 cut(s) 1609
PleI GAGTC 9 cut(s) 1505, 2029, 2668, 3466, 3648, 4029, 4133, 4510, 4633
PluTI GGCGCC 1 cut(s) 3949
PpsI GAGTC 9 cut(s) 1505, 2029, 2668, 3466, 3648, 4029, 4133, 4510, 4633
Ppu21I YACGTR 2 cut(s) 953, 2579
PpuMI RGGWCCY 1 cut(s) 2438
PsiI TTATAA 1 cut(s) 2652
Psp1406I AACGTT 2 cut(s) 2299, 2449
Psp5II RGGWCCY 1 cut(s) 2438
Psp6I CCWGG 4 cut(s) 45, 298, 1081, 3592
PspFI CCCAGC 3 cut(s) 418, 504, 3353
PspGI CCWGG 4 cut(s) 45, 298, 1081, 3592
PspN4I GGNNCC 9 cut(s) 51, 1303, 2141, 2439, 2838, 3457, 3574, 3947, 4667
PspPI GGNCC 4 cut(s) 98, 1079, 2071, 2438
PspPPI RGGWCCY 1 cut(s) 2438
PspXI VCTCGAGB 2 cut(s) 2693, 4622
PstI CTGCAG 3 cut(s) 1242, 1955, 4071
PstNI CAGNNNCTG 1 cut(s) 4034
PsuI RGATCY 4 cut(s) 49, 1606, 3572, 4131
PsyI GACNNNGTC 1 cut(s) 888
RsaI GTAC 7 cut(s) 1656, 2056, 2891, 2904, 3748, 4461, 4611
RsaNI GTAC 7 cut(s) 1655, 2055, 2890, 2903, 3747, 4460, 4610
RseI CAYNNNNRTG 4 cut(s) 606, 1269, 1449, 2259
SalI GTCGAC 1 cut(s) 4424
Sau96I GGNCC 4 cut(s) 98, 1079, 2071, 2438
SchI GAGTC 9 cut(s) 1506, 2029, 2669, 3466, 3649, 4030, 4133, 4511, 4634
ScrFI CCNGG 7 cut(s) 47, 96, 199, 300, 1083, 1611, 3594
SduI GDGCHC 5 cut(s) 2920, 3008, 3342, 3819, 3991
SfcI CTRYAG 7 cut(s) 1238, 1680, 1951, 2271, 3905, 4067, 4186
SfoI GGCGCC 1 cut(s) 3947
Sfr274I CTCGAG 2 cut(s) 2693, 4622
SinI GGWCC 4 cut(s) 98, 1079, 2071, 2438
SlaI CTCGAG 2 cut(s) 2693, 4622
SmiI ATTTAAAT 1 cut(s) 3972
SmiMI CAYNNNNRTG 4 cut(s) 606, 1269, 1449, 2259
SmlI CTYRAG 8 cut(s) 670, 2693, 3295, 4307, 4409, 4553, 4622, 4697
SmoI CTYRAG 8 cut(s) 670, 2693, 3295, 4307, 4409, 4553, 4622, 4697
SphI GCATGC 4 cut(s) 1268, 1847, 1960, 4435
SseBI AGGCCT 2 cut(s) 3204, 4542
SspDI GGCGCC 1 cut(s) 3945
SspI AATATT 2 cut(s) 2029, 3134
SspMI CTAG 9 cut(s) 893, 1355, 1481, 1517, 1914, 2258, 3287, 3420, 4418
StuI AGGCCT 2 cut(s) 3204, 4542
StyD4I CCNGG 7 cut(s) 45, 94, 197, 298, 1081, 1609, 3592
StyI CCWWGG 2 cut(s) 681, 3806
SwaI ATTTAAAT 1 cut(s) 3972
TaqII GACCGA 2 cut(s) 1067, 4280
TatI WGTACW 4 cut(s) 1654, 2054, 2889, 4459
TauI GCSGC 4 cut(s) 22, 25, 28, 934
TseFI GTSAC 6 cut(s) 2799, 2809, 2969, 3469, 4469, 4701
TseI GCWGC 6 cut(s) 557, 849, 985, 1240, 1950, 1953
Tsp45I GTSAC 6 cut(s) 2799, 2809, 2969, 3469, 4469, 4701
TspGWI ACGGA 3 cut(s) 76, 2949, 3661
Tth111I GACNNNGTC 1 cut(s) 888
Van91I CCANNNNNTGG 1 cut(s) 2084
Vha464I CTTAAG 2 cut(s) 670, 4307
VneI GTGCAC 1 cut(s) 3815
VpaK11BI GGWCC 4 cut(s) 98, 1079, 2071, 2438
XagI CCTNNNNNAGG 1 cut(s) 3209
XbaI TCTAGA 1 cut(s) 2257
XceI RCATGY 7 cut(s) 1256, 1268, 1440, 1847, 1851, 1960, 4435
XcmI CCANNNNNNNNNTGG 1 cut(s) 2081
XhoI CTCGAG 2 cut(s) 2693, 4622
XmiI GTMKAC 1 cut(s) 4425
XmnI GAANNNNTTC 5 cut(s) 273, 497, 829, 1182, 1233
XspI CTAG 9 cut(s) 893, 1355, 1481, 1517, 1914, 2258, 3287, 3420, 4418
Zsp2I ATGCAT 1 cut(s) 1849
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.