Rroxscaffold_3G00219870

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000129
Physical Location & Seq
Reverse (-)
2070650 .. 2075699
5050 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00219870.1

Sequence Viewer

Length: 3711 bp
ATGACTACCAGAAGAGATCTTCCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTGTCCTCCTCCTCCATTTCGTAAATTGACATACGATGTGTTCCTCAGTTTCAGAGGTAAAGATACCCGCACAAATTTCACAGACCATCTTTATACCGCTCTCATACAGAAAGGAATAAACACATTTAGAGATGATGAAGAACTTGAGAGAGGGGGACCAATTGGTCCAAATCTCTTGACAGCAATTGAAGAATCAAGATATGCTATTGTGGTTTTTTCCCAGAACTACGCCGATTCTTCGTGGTGTTTGGACGAGCTTGCAAAAGTCGCTGAATGTAGCAGAGTCTCAGGACAAACAGTCCTCCCTGTGTTCTACCATGTTGAGCCGTTTGAGGTACGAAGACAAAAAGGCGATCGCTTTGAGAAAGCATTTGAGAAGCATCAACAGCGGCATCCAGACAAATTGCAGAGGTGGAAAGACGCTCTTGCTGAAGTAGGCAATATCTCTGGATGGCATCTGAAAGATGGGTACGAATCCAAAGTCATTCACGAAATTGTTGAAAAGATTTTCACCGAGTTAAATCAAACAATCACAATATCTGAGGATTTAGTTGGGATGGATTCTCACCTGAATGAAATGCTTTCGTACTTAGACATTGGATGTCCTGATGTTCGTATCATAGGGATTTGTGGTATGGGTGGTATCGGTAAGACAACTATTGCACAAGTGGTTTTTGAAAGGGTACAGGCTCAGTTTGAAGGTTGCAGCTTTCTTGAGAATGTTAGAGATGTAACTGAAAAGCAAGGTGCGGTTCATTTACAAGAGCAACTTCTTTTGAATTTGCTGAAATGTATTGTAAATGTACAGAACACTAGAATGGGAAAAAATATAATGAGGCAGAGACTAAGTACTAAAATGGTTCTTATCATTCTTGATGATGTGGACCAAGAAGAACAATTGGAAGCATTGTGTGATAGGGAATCGTTTGGTCCAGGGAGTAGAATCATCATAACTTCTAGAGATGAACATTTGCTCAATGCAGTTGAAGGAGATAAAGTGTACAAGGTGAATCCATTAAGTGATGCTGAAGCTCTTCAGCTTTTCAGTATGAAAGCCTTCAAGAAAGACCAGCAGGTTGGGAAAGAATTTCTCCAGCTATCCAAGGAATTTTTGAAATATGCTGATGGCCTTCCACTAGCTATTAAAGTTTTGGGTTCCTCAGTTCGTGGTAAAAATGTAAAATTATGGTCTAGTGCATTGAATAGACTTAATAAAAACCCTCAAAATCAAAAAAAGATTATTTATGTTCTTAAAGTCAGTTTTGATGGATTAGAAGAAACAGAAAAGAAAACGTTTTTGGACATCGCGTGTTTCTTTAAAGGAGAGAACAAGGATCGTGTAATAAGAATACTAAATGGTTGCGACGATGACGGTGTCGAGATGGATGTACAAGTTCTTATGGACAAATCTTTGGTAACTCTATTTGGAACAAAATTGTGGATGCATGATTTGATACAAGAAATGGGTCGGAATGTTGTTCTTCAAGAATCTCCTAATCCTGGAGAACGTAGCAGATTGTGGCTTCCAAATGAGATTATCCCTGTACTTGATCGGAGTAAGGCGACGAGTGAAGTTCAAAGCATATTCCTCCAATGCCCAACAGAAGACGATGTTGTTCACTCAATTGATAATGCCTTCTCAAATATGGACAGATTGAGATTGCTCAAGATTGTGAATGTGAACTTTTCCGGAAACATCACATATCTTTCTAATGAGTTACAATATCTTGAATGGAAAGACTGTCCTTTGGTTTCTTTCCCGTCAGACTTTCAACCGGAGAAACTCGTTGAAGTTTGCATGTATTTTAGCCGCATTGAACAACTATGGAAGGGGAAAAAAGGATGGAGCATGCTACGACATTTAGATCTGACTGGTTCACCATTCTTGACGTCGACTCCAGACTTCACTGAGGTTCCAGATCTTGAAGAGTTGGTGCTTCAATTTTGTAGAAACTTGGTGGAAGTTCACCCATCTCTTGGATTTCTGAAGAAACTTGTCTCTTTGAATATGAGAAATTGCGAGTCTATTGAGAGCATTCCACCTTTCACTGCCCTTGAATCCCTTCAAATAGTGTGTCTTTCATCCTGTTCAGGACTGAAGAAGTTTCCAGAAATTGAAGGAAATATGAAAAGCTTGCTGGAGCTTCATTTGGATGGGACCAGCATTGAGTCATTGCCCCCATCAATTGAACGCTTGACTGGTCTTACCATTTTGAATCTAGCAGACTGTAAAAACCTTTTGCATCTTCCCAATACCATTGGCTGCTTGACATCTTTGAAAAGTCTCCATCTAACTGGTTGCTACAAAATTGATGAGATACCTGAGAATCTGAATGGTATGAAATGTCTGGAGAGCCTTGCAATTGATGGAACTTCAATAAGGGAGTTATCTTTCATTCTAGGCATGAAGAATCTAAAGTATATATCCTGCGAAGGATGTAAATGTCTAGTTCCAGAATCATGCAAAGTGCTGGCTTCGTTACCGAATTTGATAGAGCTAGACTTAAGTTATTGCAATCTGATGGATGGAGCAATTCTCAATGATTTTAGCAGCCTAATCTCCTTAAGAAGGTTAGATTTAAATGGTAATTGCTTTGTGCGATTACCTGAAAGTATCTCTCATCTCTCAAAGCTCGACACTCTGGACTTGAGTAACTGCAGACAGCTTCAATTGCTGCCCAAGAAGCTTCCGTTAAGTCTTCAGTACGTGTATGCACAAGATTGTACTTCACTGACGGATTACCCAAATCAAATCAAAGTATTGAATTCATTGGAGTGGGGAGTGACTGTTGTCAATTCCCTCAATTCTTCAGCACTTGGAGTCCCACAAAGTTCCGAAATGGCTAATTTAAGAGATGAGTGGGAATCTACAGACCTGTGTTCCACAATGTTTACTAATCCAGAAAATGTGGGGGACTCTTTCCAACTGCGTGTGACATGTAAGCATACAGAGGGGAAACAAGTTGATCATTTGCCAGTGACAACTCTAGCGTCTATAAAACAAATGCCTTTCGAGTCAGATATAAGGATCGTACATCCTGGAGATGAAATTCCAAAGTGGTTCAGCACTATATCTACCAGAAATCCCATATCAATCTCGATACCTCAAAATCTGGCCGATGATAACATGTGGAGGGGATTTGCCGCGTGTGCTATATTCTCAGTCAGGGGGCATACTGCTCTCTCTGTTATTGAACCGGATCCTGATTTTCTTAATTACTCGTATCAAATCACATTGGAAACTGATGTTATGCATCTGGAACCATTTCGGGTGTTGGGAGTGGATTTAGGCGGCTGCCGTGGCTCTGAATCAAGTTCTCATCTGGTTGTGATTTTCGGTATATCTAGTTCGAAGTTTCCACAGAGGGGGTTAAATCAATCAACTGTGGTGAGGGCTATATTTGAAACTACCAATCCATCCATGGTGGTCCAGAAATGTGGAATTCGTCTACTCTATGAGCAAGATGCTGGATGGCATATCAAAATAGCCGGGGGCGAGGATCATCCGATTTGTCTGAATAAAGTTGAGGATGCCGCCTCTGTACCGCAGTCTGCCTGCTGGCTTAATCAATCTTTGGAATGGGAGAAATTCATACCACCCTTGGAAGAAGAATCCACACTTGTGCTGATAAAGGCTATGTTTGGTATGGCTGTTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1236

Amino Acids

138.98

Weight (kDa)

5.31

Isoelectric Point (pI)

46.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 28 - 197 7.6e-54 TIR domain
TIR_2 PF13676 30 - 126 1.9e-14 TIR domain
NB-ARC PF00931 207 - 374 1.1e-23 NB-ARC domain
WHD_ROQ1 PF23282 442 - 513 3e-15 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 702 - 789 1.1e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 715 - 782 1.1e-06 Leucine-rich repeat region
LRR_14 PF23598 841 - 942 2.4e-09 Leucine-rich repeat region
C-JID PF20160 1029 - 1173 4.5e-10 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 213
AatII GACGTC 1 cut(s) 1943
Acc36I ACCTGC 1 cut(s) 1114
AccB7I CCANNNNNTGG 1 cut(s) 2027
AccBSI CCGCTC 1 cut(s) 149
AccI GTMKAC 2 cut(s) 1943, 3499
AccII CGCG 2 cut(s) 1358, 3197
AccIII TCCGGA 1 cut(s) 1739
AciI CCGC 9 cut(s) 118, 147, 439, 800, 1861, 3195, 3342, 3585, 3596
AclI AACGTT 1 cut(s) 1343
AclWI GGATC 5 cut(s) 1392, 3086, 3245, 3258, 3558
AcoI YGGCCR 1 cut(s) 3165
AcsI RAATTY 9 cut(s) 124, 829, 1136, 1157, 2536, 2815, 3099, 3492, 3638
AcuI CTGAAG 7 cut(s) 501, 1070, 1098, 2057, 2168, 2735, 2844
AcyI GRCGYC 1 cut(s) 1940
AfiI CCNNNNNNNGG 4 cut(s) 1550, 2027, 2619, 3416
AflII CTTAAG 2 cut(s) 2554, 2614
AflIII ACRYGT 3 cut(s) 2757, 2987, 3177
AhdI GACNNNNNGTC 1 cut(s) 347
AjnI CCWGG 3 cut(s) 982, 1549, 3088
AjuI GAANNNNNNNTTGG 2 cut(s) 1331, 1363
AleI CACNNNNGTG 1 cut(s) 3671
Alw26I GTCTC 4 cut(s) 340, 886, 2053, 2339
AlwI GGATC 5 cut(s) 1392, 3086, 3245, 3258, 3558
Aor13HI TCCGGA 1 cut(s) 1739
AoxI GGCC 2 cut(s) 1177, 3165
ApeKI GCWGC 5 cut(s) 756, 2313, 2601, 2725, 3345
ApoI RAATTY 9 cut(s) 124, 829, 1136, 1157, 2536, 2815, 3099, 3492, 3638
ArsI GACNNNNNNTTYG 4 cut(s) 516, 548, 2110, 2142
AsiSI GCGATCGC 1 cut(s) 406
Asp700I GAANNNNTTC 4 cut(s) 1083, 1106, 2112, 3315
AspS9I GGNCC 6 cut(s) 206, 215, 934, 980, 2208, 3478
AsuC2I CCSGG 1 cut(s) 3541
AsuHPI GGTGA 6 cut(s) 553, 608, 1069, 1920, 2009, 3451
AsuII TTCGAA 1 cut(s) 3401
AvaII GGWCC 6 cut(s) 206, 215, 934, 980, 2208, 3478
BamHI GGATCC 1 cut(s) 3250
BbsI GAAGAC 3 cut(s) 397, 1662, 2741
BbvI GCAGC 5 cut(s) 768, 2300, 2613, 2712, 3332
BceAI ACGGC 2 cut(s) 361, 3333
BciT130I CCWGG 3 cut(s) 984, 1551, 3090
BclI TGATCA 1 cut(s) 3016
BcnI CCSGG 1 cut(s) 3541
BcoDI GTCTC 4 cut(s) 340, 886, 2053, 2339
BfmI CTRYAG 2 cut(s) 2707, 2919
BfrI CTTAAG 2 cut(s) 2554, 2614
BfuAI ACCTGC 1 cut(s) 1114
BglII AGATCT 3 cut(s) 16, 1915, 1969
BmcAI AGTACT 1 cut(s) 901
Bme1390I CCNGG 4 cut(s) 984, 1551, 3090, 3541
Bme18I GGWCC 6 cut(s) 206, 215, 934, 980, 2208, 3478
BmeRI GACNNNNNGTC 1 cut(s) 347
BmgT120I GGNCC 6 cut(s) 206, 215, 934, 980, 2208, 3478
BmiI GGNNCC 6 cut(s) 207, 1207, 1965, 2209, 3252, 3312
BmrFI CCNGG 4 cut(s) 984, 1551, 3090, 3541
BmsI GCATC 9 cut(s) 439, 451, 514, 1063, 1482, 2302, 3313, 3505, 3571
BoxI GACNNNNGTC 1 cut(s) 2840
BpiI GAAGAC 3 cut(s) 397, 1662, 2741
BplI GAGNNNNNCTC 2 cut(s) 2571, 2603
BpmI CTGGAG 6 cut(s) 1127, 1572, 1932, 2210, 2420, 3111
Bpu14I TTCGAA 1 cut(s) 3401
BpuEI CTTGAG 4 cut(s) 215, 785, 1700, 2719
BpuMI CCSGG 1 cut(s) 3541
BsaAI YACGTR 1 cut(s) 2758
BsaHI GRCGYC 1 cut(s) 1940
BsaJI CCNNGG 6 cut(s) 983, 1152, 3349, 3471, 3540, 3651
BsaWI WCCGGW 3 cut(s) 1739, 1825, 3247
BsaXI ACNNNNNCTCC 6 cut(s) 1032, 1062, 1930, 1960, 2823, 2853
Bsc4I CCNNNNNNNGG 4 cut(s) 1550, 2027, 2619, 3416
Bse1I ACTGG 4 cut(s) 1927, 2254, 2350, 3026
Bse3DI GCAATG 1 cut(s) 2222
BseAI TCCGGA 1 cut(s) 1739
BseBI CCWGG 3 cut(s) 984, 1551, 3090
BseDI CCNNGG 6 cut(s) 983, 1152, 3349, 3471, 3540, 3651
BseLI CCNNNNNNNGG 4 cut(s) 1550, 2027, 2619, 3416
BseMI GCAATG 1 cut(s) 2222
BseMII CTCAG 8 cut(s) 109, 351, 582, 755, 1224, 1950, 2364, 3225
BseNI ACTGG 4 cut(s) 1927, 2254, 2350, 3026
BseRI GAGGAG 2 cut(s) 48, 51
BseXI GCAGC 5 cut(s) 768, 2300, 2613, 2712, 3332
Bsh1236I CGCG 2 cut(s) 1358, 3197
Bsh1285I CGRYCG 1 cut(s) 406
BshFI GGCC 2 cut(s) 1179, 3167
BsiEI CGRYCG 1 cut(s) 406
BsiSI CCGG 4 cut(s) 1740, 1826, 3248, 3540
BslFI GGGAC 4 cut(s) 219, 2221, 2858, 2978
BslI CCNNNNNNNGG 4 cut(s) 1550, 2027, 2619, 3416
BsmAI GTCTC 4 cut(s) 340, 886, 2053, 2339
BsmFI GGGAC 4 cut(s) 219, 2221, 2858, 2978
BsmI GAATGC 1 cut(s) 2085
BsnI GGCC 2 cut(s) 1179, 3167
Bsp119I TTCGAA 1 cut(s) 3401
Bsp13I TCCGGA 1 cut(s) 1739
Bsp1407I TGTACA 3 cut(s) 853, 1050, 1438
Bsp19I CCATGG 1 cut(s) 3471
BspACI CCGC 9 cut(s) 118, 147, 439, 800, 1861, 3195, 3342, 3585, 3596
BspANI GGCC 2 cut(s) 1179, 3167
BspCNI CTCAG 8 cut(s) 108, 350, 583, 754, 1223, 1951, 2365, 3224
BspEI TCCGGA 1 cut(s) 1739
BspFNI CGCG 2 cut(s) 1358, 3197
BspLI GGNNCC 6 cut(s) 207, 1207, 1965, 2209, 3252, 3312
BspMAI CTGCAG 1 cut(s) 2711
BspMI ACCTGC 1 cut(s) 1114
BspPI GGATC 5 cut(s) 1392, 3086, 3245, 3258, 3558
BspQI GCTCTTC 1 cut(s) 1089
BspT104I TTCGAA 1 cut(s) 3401
BspTI CTTAAG 2 cut(s) 2554, 2614
BsrBI CCGCTC 1 cut(s) 149
BsrDI GCAATG 1 cut(s) 2222
BsrGI TGTACA 3 cut(s) 853, 1050, 1438
BsrI ACTGG 4 cut(s) 1927, 2254, 2350, 3026
BssECI CCNNGG 6 cut(s) 983, 1152, 3349, 3471, 3540, 3651
BssNI GRCGYC 1 cut(s) 1940
BssT1I CCWWGG 3 cut(s) 1152, 3471, 3651
Bst2UI CCWGG 3 cut(s) 984, 1551, 3090
Bst4CI ACNGT 6 cut(s) 349, 1424, 1793, 2279, 2839, 3436
Bst6I CTCTTC 3 cut(s) 7, 1089, 1971
BstACI GRCGYC 1 cut(s) 1940
BstAFI CTTAAG 2 cut(s) 2554, 2614
BstAUI TGTACA 3 cut(s) 853, 1050, 1438
BstBAI YACGTR 1 cut(s) 2758
BstBI TTCGAA 1 cut(s) 3401
BstC8I GCNNGC 6 cut(s) 309, 1901, 2186, 2523, 3607, 3611
BstDSI CCRYGG 2 cut(s) 3349, 3471
BstENI CCTNNNNNAGG 1 cut(s) 2617
BstFNI CGCG 2 cut(s) 1358, 3197
BstMAI GTCTC 4 cut(s) 340, 886, 2053, 2339
BstMCI CGRYCG 1 cut(s) 406
BstMWI GCNNNNNNNGC 6 cut(s) 317, 436, 2722, 2734, 3200, 3351
BstNI CCWGG 3 cut(s) 984, 1551, 3090
BstNSI RCATGY 4 cut(s) 1852, 1903, 2991, 3181
BstPAI GACNNNNGTC 1 cut(s) 2840
BstSCI CCNGG 4 cut(s) 982, 1549, 3088, 3539
BstSFI CTRYAG 2 cut(s) 2707, 2919
BstUI CGCG 2 cut(s) 1358, 3197
BstV1I GCAGC 5 cut(s) 768, 2300, 2613, 2712, 3332
BstV2I GAAGAC 3 cut(s) 397, 1662, 2741
BstX2I RGATCY 4 cut(s) 16, 1915, 1969, 3250
BstXI CCANNNNNNTGG 3 cut(s) 1127, 2345, 3488
BstYI RGATCY 4 cut(s) 16, 1915, 1969, 3250
BsuRI GGCC 2 cut(s) 1179, 3167
BtgI CCRYGG 2 cut(s) 3349, 3471
BtgZI GCGATG 1 cut(s) 1339
BtsI GCAGTG 1 cut(s) 2097
BtsIMutI CAGTG 4 cut(s) 1956, 2097, 2780, 3033
BveI ACCTGC 1 cut(s) 1114
Cac8I GCNNGC 6 cut(s) 309, 1901, 2186, 2523, 3607, 3611
Cfr13I GGNCC 6 cut(s) 206, 215, 934, 980, 2208, 3478
CseI GACGC 2 cut(s) 479, 3030
CviAII CATG 9 cut(s) 368, 1496, 1849, 1900, 2455, 2511, 2988, 3178, 3472
DraI TTTAAA 2 cut(s) 1369, 2631
DrdI GACNNNNNNGTC 1 cut(s) 213
DriI GACNNNNNGTC 1 cut(s) 347
DseDI GACNNNNNNGTC 1 cut(s) 213
EaeI YGGCCR 1 cut(s) 3165
Eam1104I CTCTTC 3 cut(s) 7, 1089, 1971
Eam1105I GACNNNNNGTC 1 cut(s) 347
EarI CTCTTC 3 cut(s) 7, 1089, 1971
Eco130I CCWWGG 3 cut(s) 1152, 3471, 3651
Eco47I GGWCC 6 cut(s) 206, 215, 934, 980, 2208, 3478
Eco57I CTGAAG 7 cut(s) 501, 1070, 1098, 2057, 2168, 2735, 2844
EcoNI CCTNNNNNAGG 1 cut(s) 2617
EcoRI GAATTC 2 cut(s) 2815, 3492
EcoRII CCWGG 3 cut(s) 982, 1549, 3088
EcoT14I CCWWGG 3 cut(s) 1152, 3471, 3651
EcoT22I ATGCAT 2 cut(s) 1497, 3306
ErhI CCWWGG 3 cut(s) 1152, 3471, 3651
FaeI CATG 9 cut(s) 371, 1499, 1852, 1903, 2458, 2514, 2991, 3181, 3475
FalI AAGNNNNNCTT 4 cut(s) 459, 491, 804, 836
FaqI GGGAC 4 cut(s) 219, 2221, 2858, 2978
FatI CATG 9 cut(s) 367, 1495, 1848, 1899, 2454, 2510, 2987, 3177, 3471
FauI CCCGC 1 cut(s) 125
FbaI TGATCA 1 cut(s) 3016
FblI GTMKAC 2 cut(s) 1943, 3499
GsuI CTGGAG 6 cut(s) 1127, 1572, 1932, 2210, 2420, 3111
HaeIII GGCC 2 cut(s) 1179, 3167
HapII CCGG 4 cut(s) 1740, 1826, 3248, 3540
HgaI GACGC 2 cut(s) 479, 3030
Hin1I GRCGYC 1 cut(s) 1940
Hin1II CATG 9 cut(s) 371, 1499, 1852, 1903, 2458, 2514, 2991, 3181, 3475
HincII GTYRAC 1 cut(s) 1944
HindII GTYRAC 1 cut(s) 1944
HindIII AAGCTT 2 cut(s) 2182, 2735
HpaII CCGG 4 cut(s) 1740, 1826, 3248, 3540
HphI GGTGA 6 cut(s) 553, 608, 1069, 1920, 2009, 3451
Hpy166II GTNNAC 9 cut(s) 934, 1051, 1669, 1732, 1928, 1944, 2017, 2943, 3500
Hpy8I GTNNAC 9 cut(s) 934, 1051, 1669, 1732, 1928, 1944, 2017, 2943, 3500
Hpy99I CGWCG 3 cut(s) 1418, 1618, 1945
HpyCH4III ACNGT 6 cut(s) 349, 1424, 1793, 2279, 2839, 3436
HpyCH4IV ACGT 4 cut(s) 1343, 1558, 1940, 2757
HpyF10VI GCNNNNNNNGC 6 cut(s) 317, 436, 2722, 2734, 3200, 3351
HpySE526I ACGT 4 cut(s) 1343, 1558, 1940, 2757
Hsp92I GRCGYC 1 cut(s) 1940
Hsp92II CATG 9 cut(s) 371, 1499, 1852, 1903, 2458, 2514, 2991, 3181, 3475
Kpn2I TCCGGA 1 cut(s) 1739
Ksp22I TGATCA 1 cut(s) 3016
LguI GCTCTTC 1 cut(s) 1089
LmnI GCTCC 3 cut(s) 1896, 2191, 2579
Lsp1109I GCAGC 5 cut(s) 768, 2300, 2613, 2712, 3332
LweI GCATC 9 cut(s) 439, 451, 514, 1063, 1482, 2302, 3313, 3505, 3571
MaeII ACGT 4 cut(s) 1343, 1558, 1940, 2757
MaeIII GTNAC 8 cut(s) 781, 1465, 1767, 2529, 2702, 2833, 2983, 3028
MbiI CCGCTC 1 cut(s) 149
MfeI CAATTG 7 cut(s) 210, 234, 947, 1674, 2235, 2412, 2720
MflI RGATCY 4 cut(s) 16, 1915, 1969, 3250
MlyI GAGTC 7 cut(s) 342, 1939, 2081, 2228, 2880, 2960, 3074
MmeI TCCRAC 2 cut(s) 1499, 2998
Mph1103I ATGCAT 2 cut(s) 1497, 3306
MroI TCCGGA 1 cut(s) 1739
MroXI GAANNNNTTC 4 cut(s) 1083, 1106, 2112, 3315
MslI CAYNNNNRTG 5 cut(s) 621, 866, 2202, 2824, 3671
MspA1I CMGCKG 1 cut(s) 439
MspCI CTTAAG 2 cut(s) 2554, 2614
MspI CCGG 4 cut(s) 1740, 1826, 3248, 3540
MspR9I CCNGG 4 cut(s) 984, 1551, 3090, 3541
MunI CAATTG 7 cut(s) 210, 234, 947, 1674, 2235, 2412, 2720
Mva1269I GAATGC 1 cut(s) 2085
MvaI CCWGG 3 cut(s) 984, 1551, 3090
MvnI CGCG 2 cut(s) 1358, 3197
MwoI GCNNNNNNNGC 6 cut(s) 317, 436, 2722, 2734, 3200, 3351
NciI CCSGG 1 cut(s) 3541
NcoI CCATGG 1 cut(s) 3471
NlaIII CATG 9 cut(s) 371, 1499, 1852, 1903, 2458, 2514, 2991, 3181, 3475
NlaIV GGNNCC 6 cut(s) 207, 1207, 1965, 2209, 3252, 3312
NmuCI GTSAC 3 cut(s) 2833, 2983, 3028
NsiI ATGCAT 2 cut(s) 1497, 3306
NspI RCATGY 4 cut(s) 1852, 1903, 2991, 3181
NspV TTCGAA 1 cut(s) 3401
OliI CACNNNNGTG 1 cut(s) 3671
PaeI GCATGC 1 cut(s) 1903
PciI ACATGT 2 cut(s) 2987, 3177
PciSI GCTCTTC 1 cut(s) 1089
PctI GAATGC 1 cut(s) 2085
PdmI GAANNNNTTC 4 cut(s) 1083, 1106, 2112, 3315
PflFI GACNNNGTC 1 cut(s) 1424
PflMI CCANNNNNTGG 1 cut(s) 2027
PfoI TCCNGGA 2 cut(s) 1549, 3088
Ple19I CGATCG 1 cut(s) 406
PleI GAGTC 7 cut(s) 341, 1939, 2080, 2227, 2879, 2960, 3073
PpsI GAGTC 7 cut(s) 341, 1939, 2080, 2227, 2879, 2960, 3073
Ppu21I YACGTR 1 cut(s) 2758
PscI ACATGT 2 cut(s) 2987, 3177
PshAI GACNNNNGTC 1 cut(s) 2840
Psp1406I AACGTT 1 cut(s) 1343
Psp6I CCWGG 3 cut(s) 982, 1549, 3088
PspGI CCWGG 3 cut(s) 982, 1549, 3088
PspN4I GGNNCC 6 cut(s) 207, 1207, 1965, 2209, 3252, 3312
PspPI GGNCC 6 cut(s) 206, 215, 934, 980, 2208, 3478
PsrI GAACNNNNNNTAC 2 cut(s) 3382, 3414
PstI CTGCAG 1 cut(s) 2711
PsuI RGATCY 4 cut(s) 16, 1915, 1969, 3250
PsyI GACNNNGTC 1 cut(s) 1424
PvuI CGATCG 1 cut(s) 406
RgaI GCGATCGC 1 cut(s) 406
RseI CAYNNNNRTG 5 cut(s) 621, 866, 2202, 2824, 3671
SalI GTCGAC 1 cut(s) 1942
SapI GCTCTTC 1 cut(s) 1089
Sau96I GGNCC 6 cut(s) 206, 215, 934, 980, 2208, 3478
ScaI AGTACT 1 cut(s) 901
SchI GAGTC 7 cut(s) 342, 1939, 2081, 2228, 2880, 2960, 3074
ScrFI CCNGG 4 cut(s) 984, 1551, 3090, 3541
SfaAI GCGATCGC 1 cut(s) 406
SfaNI GCATC 9 cut(s) 439, 451, 514, 1063, 1482, 2302, 3313, 3505, 3571
SfcI CTRYAG 2 cut(s) 2707, 2919
SfuI TTCGAA 1 cut(s) 3401
SgfI GCGATCGC 1 cut(s) 406
SinI GGWCC 6 cut(s) 206, 215, 934, 980, 2208, 3478
SmiI ATTTAAAT 1 cut(s) 2631
SmiMI CAYNNNNRTG 5 cut(s) 621, 866, 2202, 2824, 3671
SmlI CTYRAG 6 cut(s) 194, 764, 1715, 2554, 2614, 2698
SmoI CTYRAG 6 cut(s) 194, 764, 1715, 2554, 2614, 2698
SphI GCATGC 1 cut(s) 1903
SsiI CCGC 9 cut(s) 118, 147, 439, 800, 1861, 3195, 3342, 3585, 3596
StyD4I CCNGG 4 cut(s) 982, 1549, 3088, 3539
StyI CCWWGG 3 cut(s) 1152, 3471, 3651
SwaI ATTTAAAT 1 cut(s) 2631
TaaI ACNGT 6 cut(s) 349, 1424, 1793, 2279, 2839, 3436
TaiI ACGT 4 cut(s) 1346, 1561, 1943, 2760
TaqI TCGA 6 cut(s) 1428, 1943, 2685, 3063, 3149, 3401
TatI WGTACW 6 cut(s) 853, 899, 1050, 1438, 1594, 2774
TauI GCSGC 5 cut(s) 442, 1863, 3197, 3345, 3587
TscAI CASTG 4 cut(s) 1963, 2104, 2787, 3033
TseFI GTSAC 3 cut(s) 2833, 2983, 3028
TseI GCWGC 5 cut(s) 756, 2313, 2601, 2725, 3345
Tsp45I GTSAC 3 cut(s) 2833, 2983, 3028
TspGWI ACGGA 2 cut(s) 2730, 2801
TspRI CASTG 4 cut(s) 1963, 2104, 2787, 3033
Tth111I GACNNNGTC 1 cut(s) 1424
Van91I CCANNNNNTGG 1 cut(s) 2027
Vha464I CTTAAG 2 cut(s) 2554, 2614
VpaK11BI GGWCC 6 cut(s) 206, 215, 934, 980, 2208, 3478
XagI CCTNNNNNAGG 1 cut(s) 2617
XapI RAATTY 9 cut(s) 124, 829, 1136, 1157, 2536, 2815, 3099, 3492, 3638
XbaI TCTAGA 1 cut(s) 1007
XceI RCATGY 4 cut(s) 1852, 1903, 2991, 3181
XcmI CCANNNNNNNNNTGG 1 cut(s) 3469
XmiI GTMKAC 2 cut(s) 1943, 3499
XmnI GAANNNNTTC 4 cut(s) 1083, 1106, 2112, 3315
ZraI GACGTC 1 cut(s) 1941
ZrmI AGTACT 1 cut(s) 901
Zsp2I ATGCAT 2 cut(s) 1497, 3306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.