Rroxscaffold_3G00235440

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000129
Physical Location & Seq
Reverse (-)
22412080 .. 22426654
14575 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00235440.1

Sequence Viewer

Length: 3666 bp
ATGATCACCCACACAGATATTCCTTCTTCTTCTTCCTCTCCTCCTCCGATCCCTCGATGGACGTACGACGTGTTCCTCAGTTTCAGGGGCGAAGATACCCGAAAAACTTTTACAGACCATCTCTACACCAGTCTTAAACAGAAAGGCATAATCACGTTCAGAGACGATGAGGGACTTGAGAGAGGAAAAGCCATTTCCCCAAAGCTGTTGAAAGCCATTGAAGAATCAAGATTCGTCATTGCCATCTTTTCAAGTAAGTACGCCGATTCAAGATGGTGCTTGGATGAGCTCGCAAAGGCTGTTGATTGTATGAACATGTTGGGACAAACGATACTCCCAGTCTTTTACCATGTGGATCCATCTGAGGTGAGAAAACAAACAGGAAAATTCGGCCAAGACTTGTGCATGCATCAACAACGCTTTAAAGACAAAATAGAGCAGGTGAATAGGTGGAGAGCTGCTCTTACTCAAGTCGCCAATCTCTCTGGATGGCATTTGCAGGATGGATCATATGAATCAAAAGTTATTCAAGAGATTGTAGGGCATATTTTCAACAAGTTGAACGAAACAATCTCAAGTGGATCCATGGATTTAGTTGGTATCGATTGTCGAATAAAACAAATCCTTTCATACTTAGACTTGGAGTTGAATGAAGTATGTATGGTAGGTATTTCGGGAATGGGGGGAATCGGTAAGACAACTATTGCACAAGTAGTTTATGAAAGGATACGTGCTCAGTTTGAGGCTTACAGCTTTCTTGCAAATGTTAGAGAGACAACTGAGAAACAGGGCCTAGTTCACTTACAAAAACAACTTCTTTCAGACATTTTGCTGGAAAGTAATGTAAATATACAGAATATTGATATGGGAGTTAATATAATAAGCCAGAGACTACGAACTAAAATGGTTCTTATCATTCTTGATGATGTGAATGACTTGCAACAACTGAAAGCATTGTGTGGTCATAGTTGGTTTGGTTCTGGGAGCAGGATCATAATAACATCAAGGGATGAACAATTGCTTCGCACATATGGAGTGGAAAGCATATACAAGGTCAAGGCATTAAACGACGCGGAAGCTCTTCAGCTCTTTAGTTGGAAAGCATTCAGGAAAGATAAGGTTGGTGAAGATTTTCTCGAGCTATCAAAGAGTGTTTTAGAATATGCTAATGGGCTTCCATTAGCTATTGAGGTTTTGGGTTCCTTACTCTTTGGTAAAAGTGTAAAAGTATGGACAAGTGCATTGGATAGACTTAAAGTTAATCCAGACAAAAGAATAATTGATGTTCTTAAAGTAACTTTTGATGCATTAGAAGACACAGAAAACGAAGTCTTTCTGGACATTGCATGTTTCTTTAAAGGGGAGGACAGAGATCGCGTAACAAGAATACTAGAAAGTAGTTGTGGCTACTCTCCAGACATTGATATAGAAGTTCTCATTGATAAGTCGATGGTAACTCTATTTGGAAGACAATTGTGGATGCATGACTTGATACAAGAATTGGGTTGGGAAATTGTTCGTCGGGAATGTCGTGATGAGCCGTGCAAACGTAGCAGGTTGTGGCTTCCAAAAGATATCATTCCTTTACTTGAACATAATAAGGGGACGAGTGCAGTGGAATCTATTTTACTGAATTTGCCAAAACAAAAGGAGGTTCACTTGAATGTTGATCCCTTCTCAAAGATGTGCAAGTTAAGATTGCTCAAAATTAATAATGCCCGCTTTTCTGGAGACATCAAATGGCTTTCTAATGAGTTACAAGTTCTCGAATGGCATGTTTGTCCTTTATATTCTTTGCCATCAAACTTTCAACCAGATAAGCTTGTTGAACTTAGCATGCATTCAAGCTGTATCAAACAGCTATGGAAGGGAAATAAGCGGTTGAGCATGCTAAAGTTCATTGATATGAGTGATTCTCAATACTTGATCAGGACCCCAAGCTTTGTTGAGGTTCCAAACATTGAAACTTTGGTGCTTGAAGGCTGTACAAGATTGGTTGAGGTTGACCCATCCATTGGAGTTCTCAAAAGACTTAATTTGTTGAATATGAGAAATTGCAAATCCCTTAAGAGCCTTCCACTTAACATTAGTTTGGAATCCCTTACAATCTTTAATCTCTCTGGCTGCTCAAGCTTGAAGAAGTTTCCGGAAATTGAAGGAAACATGCAGAGCTTGTTGGAGCTTCATCTAGATGGGACTGCCATAGAGGAATTACCTGCATCAATTGAAAGTTTAACTGGTCTCACTTTGTTGAATTTAGGAGACTGCAAAAATCTGTTCCGGCTTCCAAGTACCATTCAATTCTTGACATCTCTGAAAAGTCTCATTCTTACAGGTTGCTCAGAACTTGGAGACATACCAGAGAATCTGAATTGTGTGGAATGTTTGGAGGATCTTGATATAAGTGGAACTGCTATAAGAGAGTTATCATACATTGCAGATATGAAGAATCTTAAGTCTCTATGTTTCAGAGGAGGTAAAAATCTACCTCCGAAATCATGGCATTCACTGTTCAATTGTTGGGGGATGTTTGGAAGGGAAGGTGTTCCTGTGGGCTTGTTGCTGCCCGCTTCCTTCTCAGGTTTGAGTTCTTTGACATATCTAAACTTCAGTGAGTGCAATCTGATGGATGGAGATATTCCAAATGATCTTGGGAGTTTAGTCTCTCTAAGAAAATTGATTTTAAGTGGTAATAAGTTTTCTTGCTTACCGGAAAGCATATCTCAGCTCTCTAAGCTTCAAACTCTCTACTTGAACAATTGTAGCCGGCTTCAATCGCTGCCTAAGAAACTCCCATTATGTGTTCGCACTGTGAATGCAGATGACTGTACATCACTGATGGATTATCCAGATCAATTCTTAGTATGGACTTCATCTGTGTCAGGAATGACAACTATGAGTTCATTGATTTCATCCAAGCATAAAAAACATTCCACTTCCTCACCGAGACAAACCTCATATCATTCCAGAACATGGATTCAGTCAACTGTCCTTGGACGTGGTTTTTTTAGTTTTAGCCATAAACCTCAAGAATATAAACCAGTTGATGATTTATCAATGCCAAATCAAGTGCTTCAGAAAGACCTTGAAGTATTGGATTACAAGTCTAGTTCCCTCATTTCCGGCTGTGAAGAAAATGAAATTCCAGAGTGGTTTGGCAATATAGATACCGGCAGCTCCATAGGAATCTCGATACCAGCAGGACTCAAAGATGACGAGCAATGGATGGGGGTTTCTGCTGTTTTCTTAATCAAGGGACATCCTCATTTCTCCGACATCAAATCAGAATCAGAAACACCTAATTACTTCTATAAATGCACAGTAGTAACTCAAGAATTTCAAGTGGAACCTGTTCTCCTTGAATGGAACCATCTCAACTGCATTCGATATAGTTCTCAGTTTCTTTGCTTTTTCTATGTACCTTGCCAATTCTTTCCCAAAAGGTTAAATGAATCAAGTGTAATGTGGGCTGTCTTTGAGACCAACAACCCATGCATGGAGGTCCAGAAATGTGGGATTCGTCTACTATTCGAGCAAGGTGTTGCAGGGTTTATCCAGACATTCATGCCTCCCACTCTTGGTATTGGAGGTCGTACGCATGATCATGGCCCTCTGCTTGCAATAAATGATCGCCGAATCATCCCCCTGACGACTGCATCAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

1221

Amino Acids

137.96

Weight (kDa)

6.07

Isoelectric Point (pI)

48.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 22 - 195 2.3e-59 TIR domain
TIR_2 PF13676 24 - 121 3.1e-16 TIR domain
NB-ARC PF00931 208 - 372 1e-20 NB-ARC domain
NACHT PF05729 223 - 364 7.4e-07 NACHT domain
WHD_ROQ1 PF23282 439 - 508 4.6e-17 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 670 - 777 9.5e-07 Leucine-rich repeat region
LRR_14 PF23598 693 - 822 3.9e-10 Leucine-rich repeat region
LRR_13 PF23286 698 - 784 7e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 859 - 930 1.4e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 430
Acc36I ACCTGC 3 cut(s) 430, 1545, 2224
AccB7I CCANNNNNTGG 2 cut(s) 2015, 2973
AccI GTMKAC 1 cut(s) 3523
AccII CGCG 2 cut(s) 1073, 1377
AccIII TCCGGA 1 cut(s) 2146
AciI CCGC 4 cut(s) 1073, 1720, 1879, 2568
AclWI GGATC 9 cut(s) 43, 350, 363, 514, 576, 589, 998, 1664, 2400
AcoI YGGCCR 1 cut(s) 391
AcsI RAATTY 5 cut(s) 386, 1633, 2254, 3141, 3335
AcuI CTGAAG 3 cut(s) 1067, 2593, 3059
AfaI GTAC 7 cut(s) 65, 260, 1987, 2293, 2830, 3420, 3595
AfiI CCNNNNNNNGG 4 cut(s) 2015, 2973, 3496, 3578
AflII CTTAAG 2 cut(s) 2066, 2453
AflIII ACRYGT 2 cut(s) 69, 315
AjiI CACGTC 2 cut(s) 70, 2999
AloI GAACNNNNNNTCC 2 cut(s) 3339, 3371
Alw21I GWGCWC 2 cut(s) 291, 736
AlwI GGATC 9 cut(s) 43, 350, 363, 514, 576, 589, 998, 1664, 2400
Ama87I CYCGRG 1 cut(s) 1136
Aor13HI TCCGGA 1 cut(s) 2146
AoxI GGCC 3 cut(s) 391, 790, 3607
ApeKI GCWGC 5 cut(s) 458, 2124, 2563, 2779, 3174
ApoI RAATTY 5 cut(s) 386, 1633, 2254, 3141, 3335
AseI ATTAAT 1 cut(s) 1710
Asp700I GAANNNNTTC 7 cut(s) 1080, 1103, 1131, 1332, 1515, 2544, 3351
AspS9I GGNCC 4 cut(s) 790, 1932, 3502, 3608
AsuHPI GGTGA 4 cut(s) 379, 454, 1136, 2934
AvaI CYCGRG 1 cut(s) 1136
AvaII GGWCC 2 cut(s) 1932, 3502
BamHI GGATCC 2 cut(s) 355, 581
BanII GRGCYC 1 cut(s) 291
BbsI GAAGAC 2 cut(s) 1320, 1474
Bbv12I GWGCWC 2 cut(s) 291, 736
BbvI GCAGC 5 cut(s) 445, 2111, 2550, 2766, 3186
BceAI ACGGC 1 cut(s) 1525
BciVI GTATCC 1 cut(s) 720
BclI TGATCA 3 cut(s) 3, 1926, 3601
BfaI CTAG 4 cut(s) 794, 1391, 2189, 3108
BfrI CTTAAG 2 cut(s) 2066, 2453
BfuAI ACCTGC 3 cut(s) 430, 1545, 2224
BfuI GTATCC 1 cut(s) 720
BisI GCNGC 5 cut(s) 459, 2125, 2564, 2780, 3175
BlsI GCNGC 5 cut(s) 460, 2126, 2565, 2781, 3176
Bme18I GGWCC 2 cut(s) 1932, 3502
BmeT110I CYCGRG 1 cut(s) 1136
BmgBI CACGTC 2 cut(s) 70, 2999
BmgT120I GGNCC 4 cut(s) 790, 1932, 3502, 3608
BmiI GGNNCC 7 cut(s) 357, 583, 1201, 1934, 1953, 3348, 3368
BmrI ACTGGG 1 cut(s) 332
BmsI GCATC 4 cut(s) 418, 1294, 1470, 2228
BmuI ACTGGG 1 cut(s) 332
BpiI GAAGAC 2 cut(s) 1320, 1474
BplI GAGNNNNNCTC 4 cut(s) 445, 477, 1900, 1932
BpmI CTGGAG 2 cut(s) 1398, 1749
BpuEI CTTGAG 6 cut(s) 197, 453, 559, 2113, 3012, 3315
Bsa29I ATCGAT 1 cut(s) 603
BsaAI YACGTR 1 cut(s) 731
BsaI GGTCTC 2 cut(s) 2246, 3473
BsaJI CCNNGG 2 cut(s) 585, 2992
BsaWI WCCGGW 2 cut(s) 2146, 2710
BsaXI ACNNNNNCTCC 2 cut(s) 3339, 3369
Bsc4I CCNNNNNNNGG 4 cut(s) 2015, 2973, 3496, 3578
Bse118I RCCGGY 2 cut(s) 2766, 3170
Bse1I ACTGG 4 cut(s) 129, 338, 2242, 3041
Bse3DI GCAATG 4 cut(s) 237, 1341, 2433, 3227
BseAI TCCGGA 1 cut(s) 2146
BseCI ATCGAT 1 cut(s) 603
BseDI CCNNGG 2 cut(s) 585, 2992
BseLI CCNNNNNNNGG 4 cut(s) 2015, 2973, 3496, 3578
BseMI GCAATG 4 cut(s) 237, 1341, 2433, 3227
BseMII CTCAG 8 cut(s) 91, 354, 749, 771, 2355, 2592, 2738, 3410
BseNI ACTGG 4 cut(s) 129, 338, 2242, 3041
BseRI GAGGAG 3 cut(s) 30, 33, 2487
BseXI GCAGC 5 cut(s) 445, 2111, 2550, 2766, 3186
BsgI GTGCAG 1 cut(s) 1632
Bsh1236I CGCG 2 cut(s) 1073, 1377
BshFI GGCC 3 cut(s) 393, 792, 3609
BshVI ATCGAT 1 cut(s) 603
BsiHKAI GWGCWC 2 cut(s) 291, 736
BsiHKCI CYCGRG 1 cut(s) 1136
BsiSI CCGG 6 cut(s) 2147, 2281, 2711, 2767, 3123, 3171
BsiWI CGTACG 2 cut(s) 63, 3593
BslFI GGGAC 5 cut(s) 186, 336, 1618, 2209, 3270
BslI CCNNNNNNNGG 4 cut(s) 2015, 2973, 3496, 3578
BsmBI CGTCTC 1 cut(s) 156
BsmFI GGGAC 5 cut(s) 186, 336, 1618, 2209, 3270
BsmI GAATGC 5 cut(s) 1103, 1840, 2503, 2821, 3381
BsnI GGCC 3 cut(s) 393, 792, 3609
Bso31I GGTCTC 2 cut(s) 2246, 3473
BsoBI CYCGRG 1 cut(s) 1136
Bsp1286I GDGCHC 2 cut(s) 291, 736
Bsp13I TCCGGA 1 cut(s) 2146
Bsp1407I TGTACA 2 cut(s) 1985, 2828
Bsp19I CCATGG 1 cut(s) 585
BspACI CCGC 4 cut(s) 1073, 1720, 1879, 2568
BspANI GGCC 3 cut(s) 393, 792, 3609
BspCNI CTCAG 8 cut(s) 90, 355, 748, 772, 2354, 2591, 2737, 3409
BspDI ATCGAT 1 cut(s) 603
BspEI TCCGGA 1 cut(s) 2146
BspFNI CGCG 2 cut(s) 1073, 1377
BspLI GGNNCC 7 cut(s) 357, 583, 1201, 1934, 1953, 3348, 3368
BspMI ACCTGC 3 cut(s) 430, 1545, 2224
BspPI GGATC 9 cut(s) 43, 350, 363, 514, 576, 589, 998, 1664, 2400
BspQI GCTCTTC 1 cut(s) 1086
BspTI CTTAAG 2 cut(s) 2066, 2453
BspTNI GGTCTC 2 cut(s) 2246, 3473
BsrDI GCAATG 4 cut(s) 237, 1341, 2433, 3227
BsrFI RCCGGY 2 cut(s) 2766, 3170
BsrGI TGTACA 2 cut(s) 1985, 2828
BsrI ACTGG 4 cut(s) 129, 338, 2242, 3041
BssAI RCCGGY 2 cut(s) 2766, 3170
BssECI CCNNGG 2 cut(s) 585, 2992
BssT1I CCWWGG 2 cut(s) 585, 2992
Bst4CI ACNGT 5 cut(s) 2511, 2812, 2828, 2989, 3322
Bst6I CTCTTC 1 cut(s) 1086
BstAFI CTTAAG 2 cut(s) 2066, 2453
BstAUI TGTACA 2 cut(s) 1985, 2828
BstBAI YACGTR 1 cut(s) 731
BstC8I GCNNGC 8 cut(s) 291, 407, 1720, 1838, 1889, 2568, 2768, 3618
BstDSI CCRYGG 1 cut(s) 585
BstFNI CGCG 2 cut(s) 1073, 1377
BstMWI GCNNNNNNNGC 4 cut(s) 1551, 2130, 2734, 2776
BstNSI RCATGY 7 cut(s) 319, 409, 1350, 1778, 1840, 1891, 2167
BstUI CGCG 2 cut(s) 1073, 1377
BstV1I GCAGC 5 cut(s) 445, 2111, 2550, 2766, 3186
BstV2I GAAGAC 2 cut(s) 1320, 1474
BstX2I RGATCY 3 cut(s) 355, 581, 2392
BstXI CCANNNNNNTGG 1 cut(s) 3512
BstYI RGATCY 3 cut(s) 355, 581, 2392
Bsu15I ATCGAT 1 cut(s) 603
BsuI GTATCC 1 cut(s) 720
BsuRI GGCC 3 cut(s) 393, 792, 3609
BsuTUI ATCGAT 1 cut(s) 603
BtgI CCRYGG 1 cut(s) 585
BtrI CACGTC 2 cut(s) 70, 2999
BtsI GCAGTG 1 cut(s) 1620
BtsIMutI CAGTG 5 cut(s) 1620, 2507, 2617, 2808, 2834
BveI ACCTGC 3 cut(s) 430, 1545, 2224
Cac8I GCNNGC 8 cut(s) 291, 407, 1720, 1838, 1889, 2568, 2768, 3618
Cfr10I RCCGGY 2 cut(s) 2766, 3170
Cfr13I GGNCC 4 cut(s) 790, 1932, 3502, 3608
ClaI ATCGAT 1 cut(s) 603
CseI GACGC 1 cut(s) 1079
Csp6I GTAC 7 cut(s) 64, 259, 1986, 2292, 2829, 3419, 3594
CviQI GTAC 7 cut(s) 64, 259, 1986, 2292, 2829, 3419, 3594
DraI TTTAAA 2 cut(s) 424, 1357
EaeI YGGCCR 1 cut(s) 391
Eam1104I CTCTTC 1 cut(s) 1086
EarI CTCTTC 1 cut(s) 1086
Ecl136II GAGCTC 1 cut(s) 289
Eco130I CCWWGG 2 cut(s) 585, 2992
Eco24I GRGCYC 1 cut(s) 291
Eco31I GGTCTC 2 cut(s) 2246, 3473
Eco32I GATATC 1 cut(s) 1576
Eco47I GGWCC 2 cut(s) 1932, 3502
Eco53kI GAGCTC 1 cut(s) 289
Eco57I CTGAAG 3 cut(s) 1067, 2593, 3059
Eco88I CYCGRG 1 cut(s) 1136
EcoICRI GAGCTC 1 cut(s) 289
EcoO109I RGGNCCY 2 cut(s) 790, 1932
EcoRV GATATC 1 cut(s) 1576
EcoT14I CCWWGG 2 cut(s) 585, 2992
EcoT22I ATGCAT 5 cut(s) 411, 1309, 1485, 1842, 3497
EcoT38I GRGCYC 1 cut(s) 291
ErhI CCWWGG 2 cut(s) 585, 2992
Esp3I CGTCTC 1 cut(s) 156
FaqI GGGAC 5 cut(s) 186, 336, 1618, 2209, 3270
FauI CCCGC 2 cut(s) 1727, 2575
FauNDI CATATG 2 cut(s) 511, 1030
FbaI TGATCA 3 cut(s) 3, 1926, 3601
FblI GTMKAC 1 cut(s) 3523
Fnu4HI GCNGC 5 cut(s) 459, 2125, 2564, 2780, 3175
FriOI GRGCYC 1 cut(s) 291
Fsp4HI GCNGC 5 cut(s) 459, 2125, 2564, 2780, 3175
FspBI CTAG 4 cut(s) 794, 1391, 2189, 3108
GluI GCNGC 5 cut(s) 459, 2125, 2564, 2780, 3175
GsuI CTGGAG 2 cut(s) 1398, 1749
HaeIII GGCC 3 cut(s) 393, 792, 3609
HapII CCGG 6 cut(s) 2147, 2281, 2711, 2767, 3123, 3171
HgaI GACGC 1 cut(s) 1079
HincII GTYRAC 2 cut(s) 2005, 2985
HindII GTYRAC 2 cut(s) 2005, 2985
HindIII AAGCTT 4 cut(s) 1820, 1939, 2131, 2735
HpaII CCGG 6 cut(s) 2147, 2281, 2711, 2767, 3123, 3171
HphI GGTGA 4 cut(s) 379, 454, 1136, 2934
Hpy166II GTNNAC 5 cut(s) 799, 1657, 2005, 2985, 3524
Hpy8I GTNNAC 5 cut(s) 799, 1657, 2005, 2985, 3524
Hpy99I CGWCG 3 cut(s) 71, 1073, 1524
HpyAV CCTTC 9 cut(s) 33, 1684, 1861, 1973, 2084, 2150, 2529, 2534, 2584
HpyCH4III ACNGT 5 cut(s) 2511, 2812, 2828, 2989, 3322
HpyCH4IV ACGT 6 cut(s) 62, 69, 155, 730, 1549, 2998
HpyF10VI GCNNNNNNNGC 4 cut(s) 1551, 2130, 2734, 2776
HpySE526I ACGT 6 cut(s) 62, 69, 155, 730, 1549, 2998
Kpn2I TCCGGA 1 cut(s) 2146
KroI GCCGGC 1 cut(s) 2766
KroNI GCCGGC 1 cut(s) 2768
Ksp22I TGATCA 3 cut(s) 3, 1926, 3601
LguI GCTCTTC 1 cut(s) 1086
LmnI GCTCC 3 cut(s) 984, 2179, 3182
Lsp1109I GCAGC 5 cut(s) 445, 2111, 2550, 2766, 3186
LweI GCATC 4 cut(s) 418, 1294, 1470, 2228
MaeI CTAG 4 cut(s) 794, 1391, 2189, 3108
MaeII ACGT 6 cut(s) 62, 69, 155, 730, 1549, 2998
MaeIII GTNAC 5 cut(s) 1294, 1378, 1453, 1755, 3325
MfeI CAATTG 5 cut(s) 1016, 1472, 2223, 2515, 2758
MflI RGATCY 3 cut(s) 355, 581, 2392
MhlI GDGCHC 2 cut(s) 291, 736
MlyI GAGTC 1 cut(s) 3198
MmeI TCCRAC 3 cut(s) 1076, 2157, 3297
Mph1103I ATGCAT 5 cut(s) 411, 1309, 1485, 1842, 3497
MroI TCCGGA 1 cut(s) 2146
MroNI GCCGGC 1 cut(s) 2766
MroXI GAANNNNTTC 7 cut(s) 1080, 1103, 1131, 1332, 1515, 2544, 3351
MslI CAYNNNNRTG 4 cut(s) 1662, 1904, 2190, 3603
MspCI CTTAAG 2 cut(s) 2066, 2453
MspI CCGG 6 cut(s) 2147, 2281, 2711, 2767, 3123, 3171
MunI CAATTG 5 cut(s) 1016, 1472, 2223, 2515, 2758
Mva1269I GAATGC 5 cut(s) 1103, 1840, 2503, 2821, 3381
MvnI CGCG 2 cut(s) 1073, 1377
MwoI GCNNNNNNNGC 4 cut(s) 1551, 2130, 2734, 2776
NaeI GCCGGC 1 cut(s) 2768
NcoI CCATGG 1 cut(s) 585
NdeI CATATG 2 cut(s) 511, 1030
NgoMIV GCCGGC 1 cut(s) 2766
NlaIV GGNNCC 7 cut(s) 357, 583, 1201, 1934, 1953, 3348, 3368
NsiI ATGCAT 5 cut(s) 411, 1309, 1485, 1842, 3497
NspI RCATGY 7 cut(s) 319, 409, 1350, 1778, 1840, 1891, 2167
PaeI GCATGC 3 cut(s) 409, 1840, 1891
PaeR7I CTCGAG 1 cut(s) 1136
PaqCI CACCTGC 1 cut(s) 430
PciI ACATGT 1 cut(s) 315
PciSI GCTCTTC 1 cut(s) 1086
PcsI WCGNNNNNNNCGW 1 cut(s) 1528
PctI GAATGC 5 cut(s) 1103, 1840, 2503, 2821, 3381
PdiI GCCGGC 1 cut(s) 2768
PdmI GAANNNNTTC 7 cut(s) 1080, 1103, 1131, 1332, 1515, 2544, 3351
Pfl23II CGTACG 2 cut(s) 63, 3593
PflMI CCANNNNNTGG 2 cut(s) 2015, 2973
PkrI GCNGC 5 cut(s) 460, 2126, 2565, 2781, 3176
PleI GAGTC 1 cut(s) 3198
PpsI GAGTC 1 cut(s) 3198
Ppu21I YACGTR 1 cut(s) 731
PpuMI RGGWCCY 1 cut(s) 1932
PscI ACATGT 1 cut(s) 315
PshBI ATTAAT 1 cut(s) 1710
Psp124BI GAGCTC 1 cut(s) 291
Psp5II RGGWCCY 1 cut(s) 1932
PspLI CGTACG 2 cut(s) 63, 3593
PspN4I GGNNCC 7 cut(s) 357, 583, 1201, 1934, 1953, 3348, 3368
PspPI GGNCC 4 cut(s) 790, 1932, 3502, 3608
PspPPI RGGWCCY 1 cut(s) 1932
PsrI GAACNNNNNNTAC 2 cut(s) 56, 88
PsuI RGATCY 3 cut(s) 355, 581, 2392
RsaI GTAC 7 cut(s) 65, 260, 1987, 2293, 2830, 3420, 3595
RsaNI GTAC 7 cut(s) 64, 259, 1986, 2292, 2829, 3419, 3594
RseI CAYNNNNRTG 4 cut(s) 1662, 1904, 2190, 3603
SacI GAGCTC 1 cut(s) 291
SapI GCTCTTC 1 cut(s) 1086
SatI GCNGC 5 cut(s) 459, 2125, 2564, 2780, 3175
Sau96I GGNCC 4 cut(s) 790, 1932, 3502, 3608
SchI GAGTC 1 cut(s) 3198
SduI GDGCHC 2 cut(s) 291, 736
SfaNI GCATC 4 cut(s) 418, 1294, 1470, 2228
Sfr274I CTCGAG 1 cut(s) 1136
SinI GGWCC 2 cut(s) 1932, 3502
SlaI CTCGAG 1 cut(s) 1136
SmiMI CAYNNNNRTG 4 cut(s) 1662, 1904, 2190, 3603
SmlI CTYRAG 9 cut(s) 176, 468, 574, 1136, 2066, 2128, 2453, 3027, 3330
SmoI CTYRAG 9 cut(s) 176, 468, 574, 1136, 2066, 2128, 2453, 3027, 3330
SphI GCATGC 3 cut(s) 409, 1840, 1891
SsiI CCGC 4 cut(s) 1073, 1720, 1879, 2568
SspI AATATT 1 cut(s) 859
SspMI CTAG 4 cut(s) 794, 1391, 2189, 3108
SstI GAGCTC 1 cut(s) 291
StyI CCWWGG 2 cut(s) 585, 2992
TaaI ACNGT 5 cut(s) 2511, 2812, 2828, 2989, 3322
TaiI ACGT 6 cut(s) 65, 72, 158, 733, 1552, 3001
TaqI TCGA 9 cut(s) 55, 603, 610, 1137, 1448, 1767, 3191, 3385, 3531
TatI WGTACW 2 cut(s) 1985, 2828
TscAI CASTG 5 cut(s) 1620, 2514, 2617, 2815, 2841
TseI GCWGC 5 cut(s) 458, 2124, 2563, 2779, 3174
TspRI CASTG 5 cut(s) 1620, 2514, 2617, 2815, 2841
Van91I CCANNNNNTGG 2 cut(s) 2015, 2973
Vha464I CTTAAG 2 cut(s) 2066, 2453
VpaK11BI GGWCC 2 cut(s) 1932, 3502
VspI ATTAAT 1 cut(s) 1710
XapI RAATTY 5 cut(s) 386, 1633, 2254, 3141, 3335
XbaI TCTAGA 1 cut(s) 2188
XceI RCATGY 7 cut(s) 319, 409, 1350, 1778, 1840, 1891, 2167
XhoI CTCGAG 1 cut(s) 1136
XmiI GTMKAC 1 cut(s) 3523
XmnI GAANNNNTTC 7 cut(s) 1080, 1103, 1131, 1332, 1515, 2544, 3351
XspI CTAG 4 cut(s) 794, 1391, 2189, 3108
Zsp2I ATGCAT 5 cut(s) 411, 1309, 1485, 1842, 3497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.