FvH4_5g38760

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000129
Physical Location & Seq
Forward (+)
28666764 .. 28672191
5428 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g38760.t1

Sequence Viewer

Length: 4356 bp
ATGTTGCACTATCTTTTATCTATACATATAATTTATGTAAGAATATTTTCATTATTTTACAGGTACGAATCCGAAGTCATTAAAATAATTGTTGAAAAGATATTTACCGACTTGAATCAACTAATCTCGAAATCTGAGGGTATAGTTGGGATAGACTCTCACCTGAATGAAATGCTTTCATACTTAGACATTGGTTGTACTGATGTTCGGATCATAGGCATTTGTGGGATGGCTGGTATCGGGAAGACCACTATTGCACAAGTGGTTTCCAAAAGGGTAAAATCTCAGTTTGAAGGTGACAGCTTCCTGGAGAATGTTAGAGAGGTAACTGAAAAGCAAGGTGCAGTTCATTTACAACAGCAACTTCTTTTGAACTTGCTGAAAAGTAATGTACATGTACAAACCAGCGACATGGGAAAAGATATAATTAGCCATAGGCTACGTACTAAAAGGGTTCTTCTCATTCTTGATGATGTGGATGAAGAAGCACAATTGGAAGCATTGTGTGATCGCACTTGGTTTGGTCCAGGGAGTAGGATCATACTTACATCAAGAGATGAGCATTTGCTCATTGCATTTGGAGTGGATAAAGTATACAAGGTGGATCCATTAAGTGATGCTGAAGCACTTCAGCTTTTCTGTATGAAAGCCTTCACGAAAGACCAGTTGGTAGGAGATGATTTTCTCAAGCTATCCAAGGAGTTCTTGAAATATGCGGATGGCAATCCATTAGCTATTAAAATTTTAGGTTCTTCTGTAAAGTGTAGAAAGGTAGAGTTATGGTTAAGTACATTGGATAAGCTAAAAAAAAGTCCAGGAAACAAAATTTTTAATGTGCTCAAAGTTTGTTTTGATGGATTAGATGAAACCCAAAAGGAAAAATTTTTGGACATTGCATGTTTCTTTAGAGGAGGGTACATAGATCAGGTAACAAGAATACTACAAGGTAGTGACGGTCCGGACATTGATATAGAAGTTCTCGTCGATAGATCTTTGGTAACTCTGTTTGGAAAAAAATTGGGGATGCATGACTTGTTAAAAGATTTGGGTTATGAAATTGTTCGTCAAGAATGCCGTGAAGAGCCTGGGAAACGTAGCAGGTTGTGGCTTGCCAATGAAATCATCAATGTACTTGGTCAGAGTAGGGCGACGAGTGCAATTCAAAGCATATACCTCCAATGTCCAACAGGAGACGATGTTGTTCACTCCATTGGTAATGCCTTCTCAAATATGGACAGATTGAGATTGCTCAAGATATCGAACGCGAATTTTTCCGGAAATATCAGTTATCTTTCTAATAAGTTGCAGTATCTTGAGTGGCATGGGTGTCCTTTGGATTCTTTCCCGTCTGACTTTCAACCGGAGGAACTTGTTGAAGTTCACATGCCTTTTAGCCGCATCAAACAACTATGGACGGGGCGAAAAGGATGGAGCATGCTCCGGCATTTGGATCTGAGTGCTTCACCATACTTGATGTCGACTCCAGACTTCACTGATGTTCCAGATCTTGAAAAGTTGGTGCTTCAATTTTGTACAAGCTTGGTGGAGATTCACCCGTCTCTTGGATTTCTGAAGAAACTTTTCATTTTGGATATGACATACTGCAAGTCCATTGAGAGCCTTCCGCCTTTCACTGCACTAGAATCCCTTCAAATATTGATGCTTTCACAATGTTCAGGACTGAAGAGGTTTCCAGAAATTGGAGGAAATATGAAAAGCTTGCTGGAGCTTTATTTGGATGGGACCAGCATTGAGGAATTGCCTTCATCCATTGAACGCTTCACTGGTCTTACCATGTTGAATCTAACAGACTGTAAAAACCTTTTGCATCTTCCCAATACCATTGGGTGCTTGACATCTTTGAAATGTCTCTATTTACAAGGTTGCTCCAAACTTGATAAGATACCGGAGAATCTGAATGGTATTGAATGTATGGAGAAGCTTTTGATTGGTGGAACTTCCATAAGGGATCTATCTATCATCGGAGGAATGAAGAATCTACAGTATCTATCCTGCCGAGGATGTAAATGTCTAGTTTCAGCATCATGTAAAGCTCTGGCTACGTTATCGAATTTGGCGCTGCTAGACTTGAGTGATTGCAGTCTGATGGATGGAGAATTTCTCAATGATTTTAGCACCATGATCTCCTTAACATTGTTAGATTTAAGTTGTAATTGTTTTGTGCGATTACCTGAAAGTCTCTCTCAATTCTCGAAGCTCCAGACTCTGCACTTGGGTAATTGCAAACAGCTTCAATTGCTGCCCGAGAAGCTTCCGTCAAGTCTTCAATGCGTGTGGGCAAAAGATTGTACGTCACTGACGGATTACCCAAATCAAATCAAAGAACTGAATTCATCGGAGTCGGGAGTGACTACTGTCATTTCCCTCAATTCTTCAGCACTTGGAATCTCAGGAATTTCCAGAAAATTGATAGTTGAGGGGGAATCTACGAAGCTGTTTTTGACACATCACCACGGAGACGGCCAAAAATTTGATCATGATAAATTCATCCCAAGCGGAGCGTATATAAAAGAAAAGATGCCTTCCTCGGCAGAATTCAGTAACGTACATGCTGGAGATGACATTCCAGAGTGGTTCAGCACTGCATCTACCAGAAATCCCATATCAATTCCATTACCTCAAAATCTGGCCGATGATAACAAGTGGAAGGGATTTGCCGCGTGCGCTGTATTCTCATTCAAGTGGCACACTGCTCTCTCCTTTGTTGAACCGGATAATAAAATTTCGAATTACTCGTACCAAATCACATGGGAAACTGATTTTGGGTGTCTCAAATCAAATGTGTTGGAGTTGAATGAACGAATTTATTTCCTTCGATCAAGTTCTCATCTGCGTATGATTTTCTATATATCTCGTTCGAAGTTTCCAGATAAGATGTTAAATCACTCAACTGTGTTAAGGGCTAAGTTTGAAACCACCGATCCATCCATGGTGGTCCAGAAATGTGGAATACGCCTGGTCTATGAGGAAGATGCTGAATGGTTTTCCAGTATGACCAACTGGGTTGAATCGGTTTGTATGAGTGAACTTGGGGATGAAGTCTTTGTACCGCAGTCTGCCTGCTGGTTTAATCCATCTTTGAAATGGGAGAGATGCATTCCACCCTTGGAAGACGAATCCACACGTGTGGTGCGAGAGAACGTTGAATCTCTTCTTCCAAGATACCTTGAGGGACTCAACAGTCGGTCTAAAATGTTCGAATTTAACCTCAGAGGAAGCCCAGGGTGGTTTGAGTTGTCTCACTGTCTCCCGGATAGTAGGTCAAATTCTGCCGCAGTGCCAATTGAGATGCCTCAAAATCTACAGAATAACAAGAAGTGGATGGGAATGGCAATACACGCTTCACTTGTGGAGGAAGTGGGACGTCAGGAGGACAGCTATTGGGTATTTGTTGGACTCAAAGCAGGTGACGGATTTGCTGACGTTTTGTTCCCAAAACCTATGCACATCAGGCCATCGTCAGAAGAACGGCATCAACTTTTGGTTTGTTATATACCGCGGGAACAGATTCCAGAAGCGGCGCCATCTCAGACATTAACAACTGCTTGTGGGTTTTCCATCAGAGTCAAAAGCCCTTGTGTAAAAGTTCAAACATGTGGATTCCGTATTGTGTACCAGGAAGATATACAAGGGTTTGCTGATACTATTCTCCAGTGCATTCAGAGGGAAGGTTCTCTTAAAGCTTACGATAAGTGGCTGGAGGAGAGAATGGCAGAGCGGGATGACGCCCATTTAGAGAGAATGACAGAACGGGATTCCAGAACCCCACGGGAGAAGTACTTTGACTTGCACTCGCAAAAATATAGAAATTGGGACTGGTGTGACCCTCGTCCTCTTTCTCGTTGCTTCGAGAGAGGTGAAATTTTCAGGTCGAAATGGTTCATGCCTTTCATCAACCAAGGAAACTCTGCAGAAATCCAGCTGCCTCCGAATCTCTTCGATGATGATAATTGGTTGGGGTTTGCCGCATGTTCTGGGTTGTCCAACAACCAATATCCAAACATTGGTCTTGGCAGCAGTAGCAGTTCTGGTCCTGATCCAGAGGAGTATGTTAGACTTACTTACCGTCTGGACTCTGATGTTGCATGGCACGGGTTATTTGATATCATGATGAAGGAAGATATTAAGTATCACAAGCTATGTGAATATGCGACTGATTTCATTTATATTCCGCGCAAAGTTCTTAATAAGGTCTGGAGGCAAAGCAAACTGGCCAGGATAACTATCTCGTTCTCTAGCCCATGCTTAGCAGTGCAAAGCTGTGCCCTTCGTCTACTATTTAAGGAGGACGTTGAACATCTGGTAGAAACATTGACCCTTGCCGAATTACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1452

Amino Acids

164.62

Weight (kDa)

5.76

Isoelectric Point (pI)

46.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 55 - 221 1.6e-22 NB-ARC domain
NACHT PF05729 72 - 216 2.3e-09 NACHT domain
WHD_ROQ1 PF23282 289 - 356 1.2e-14 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 536 - 689 2.2e-12 Leucine-rich repeat region
LRR_13 PF23286 547 - 633 2e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 858 - 1000 2.9e-13 C-JID domain
C-JID PF20160 1300 - 1439 8.3e-06 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3416
AasI GACNNNNNNGTC 1 cut(s) 3201
AatII GACGTC 1 cut(s) 3388
Acc36I ACCTGC 2 cut(s) 1089, 3416
AccB1I GGYRCC 1 cut(s) 3541
AccB7I CCANNNNNTGG 2 cut(s) 1700, 4025
AccBSI CCGCTC 1 cut(s) 3739
AccI GTMKAC 3 cut(s) 594, 1478, 4294
AccII CGCG 4 cut(s) 1265, 2681, 3520, 4195
AccIII TCCGGA 2 cut(s) 958, 1274
AclI AACGTT 1 cut(s) 3162
AclWI GGATC 8 cut(s) 218, 545, 599, 612, 1458, 1977, 2936, 4052
AcoI YGGCCR 3 cut(s) 2482, 2649, 4233
AcuI CTGAAG 5 cut(s) 614, 642, 1592, 1703, 2379
AcvI CACGTG 1 cut(s) 3146
AcyI GRCGYC 3 cut(s) 3385, 3542, 3747
AfiI CCNNNNNNNGG 4 cut(s) 1447, 1562, 1700, 4025
AflIII ACRYGT 4 cut(s) 394, 3143, 3145, 3614
AjnI CCWGG 8 cut(s) 306, 526, 814, 1084, 2976, 3241, 3636, 4235
AjuI GAANNNNNNNTTGG 4 cut(s) 650, 682, 2766, 2798
AleI CACNNNNGTG 1 cut(s) 3146
Alw21I GWGCWC 1 cut(s) 840
Alw26I GTCTC 8 cut(s) 1185, 1563, 1874, 2204, 2472, 2795, 3264, 3272
AlwI GGATC 8 cut(s) 218, 545, 599, 612, 1458, 1977, 2936, 4052
AlwNI CAGNNNCTG 1 cut(s) 2227
Ama87I CYCGRG 1 cut(s) 2264
Aor13HI TCCGGA 2 cut(s) 958, 1274
AoxI GGCC 4 cut(s) 2482, 2649, 3473, 4233
ApeKI GCWGC 4 cut(s) 2080, 2260, 3943, 4035
ArsI GACNNNNNNTTYG 2 cut(s) 60, 92
AspLEI GCGC 4 cut(s) 2080, 2687, 3544, 4197
AspS9I GGNCC 5 cut(s) 524, 956, 1743, 2956, 4052
AsuC2I CCSGG 1 cut(s) 3272
AsuHPI GGTGA 7 cut(s) 152, 308, 1455, 1544, 2462, 3440, 3890
AsuII TTCGAA 3 cut(s) 2747, 2879, 3219
AvaI CYCGRG 1 cut(s) 2264
AvaII GGWCC 5 cut(s) 524, 956, 1743, 2956, 4052
BaeGI GKGCMC 1 cut(s) 4288
BalI TGGCCA 1 cut(s) 4235
BamHI GGATCC 1 cut(s) 604
BanI GGYRCC 1 cut(s) 3541
BarI GAAGNNNNNNTAC 2 cut(s) 3051, 3083
BbrPI CACGTG 1 cut(s) 3146
BbsI GAAGAC 3 cut(s) 251, 2276, 3138
Bbv12I GWGCWC 1 cut(s) 840
BbvI GCAGC 4 cut(s) 2067, 2247, 3930, 4047
BceAI ACGGC 3 cut(s) 1059, 2497, 3506
BciT130I CCWGG 8 cut(s) 308, 528, 816, 1086, 2978, 3243, 3638, 4237
BclI TGATCA 1 cut(s) 2494
BcnI CCSGG 1 cut(s) 3272
BcoDI GTCTC 8 cut(s) 1185, 1563, 1874, 2204, 2472, 2795, 3264, 3272
BfaI CTAG 4 cut(s) 1640, 2033, 2084, 4257
BfmI CTRYAG 3 cut(s) 2000, 3323, 3930
BfoI RGCGCY 2 cut(s) 2081, 3545
BfuAI ACCTGC 2 cut(s) 1089, 3416
BglII AGATCT 2 cut(s) 989, 1504
BisI GCNGC 9 cut(s) 1396, 2081, 2261, 2679, 3294, 3540, 3944, 3987, 4036
BlpI GCTNAGC 1 cut(s) 4267
BlsI GCNGC 9 cut(s) 1397, 2082, 2262, 2680, 3295, 3541, 3945, 3988, 4037
BmcAI AGTACT 1 cut(s) 3800
Bme1390I CCNGG 9 cut(s) 308, 528, 816, 1086, 2978, 3243, 3272, 3638, 4237
Bme18I GGWCC 5 cut(s) 524, 956, 1743, 2956, 4052
BmeT110I CYCGRG 1 cut(s) 2264
BmgT120I GGNCC 5 cut(s) 524, 956, 1743, 2956, 4052
BmiI GGNNCC 3 cut(s) 606, 1744, 3543
BmrFI CCNGG 9 cut(s) 308, 528, 816, 1086, 2978, 3243, 3272, 3638, 4237
BmrI ACTGGG 1 cut(s) 3031
BmuI ACTGGG 1 cut(s) 3031
BoxI GACNNNNGTC 2 cut(s) 2375, 3849
BpiI GAAGAC 3 cut(s) 251, 2276, 3138
BplI GAGNNNNNCTC 2 cut(s) 2106, 2138
BpmI CTGGAG 8 cut(s) 329, 1467, 1745, 2204, 2595, 3656, 3740, 4237
Bpu1102I GCTNAGC 1 cut(s) 4267
Bpu14I TTCGAA 3 cut(s) 2747, 2879, 3219
BpuEI CTTGAG 5 cut(s) 671, 1235, 1334, 2110, 3209
BpuMI CCSGG 1 cut(s) 3272
BsaAI YACGTR 2 cut(s) 443, 3146
BsaBI GATNNNNATC 2 cut(s) 723, 4244
BsaHI GRCGYC 3 cut(s) 3385, 3542, 3747
BsaWI WCCGGW 5 cut(s) 958, 1274, 1360, 1906, 2731
BsaXI ACNNNNNCTCC 2 cut(s) 1182, 1212
Bsc4I CCNNNNNNNGG 4 cut(s) 1447, 1562, 1700, 4025
Bse1I ACTGG 7 cut(s) 664, 1789, 3009, 3026, 3673, 3842, 4236
Bse3DI GCAATG 2 cut(s) 570, 891
Bse8I GATNNNNATC 2 cut(s) 723, 4244
BseAI TCCGGA 2 cut(s) 958, 1274
BseBI CCWGG 8 cut(s) 308, 528, 816, 1086, 2978, 3243, 3638, 4237
BseJI GATNNNNATC 2 cut(s) 723, 4244
BseLI CCNNNNNNNGG 4 cut(s) 1447, 1562, 1700, 4025
BseMI GCAATG 2 cut(s) 570, 891
BseMII CTCAG 6 cut(s) 126, 299, 1445, 2424, 3244, 3563
BseNI ACTGG 7 cut(s) 664, 1789, 3009, 3026, 3673, 3842, 4236
BseRI GAGGAG 3 cut(s) 924, 3737, 4079
BseSI GKGCMC 1 cut(s) 4288
BseXI GCAGC 4 cut(s) 2067, 2247, 3930, 4047
BsgI GTGCAG 3 cut(s) 363, 1620, 2213
Bsh1236I CGCG 4 cut(s) 1265, 2681, 3520, 4195
BshFI GGCC 4 cut(s) 2484, 2651, 3475, 4235
BshNI GGYRCC 1 cut(s) 3541
BsiHKAI GWGCWC 1 cut(s) 840
BsiHKCI CYCGRG 1 cut(s) 2264
BsiSI CCGG 7 cut(s) 959, 1275, 1361, 1441, 1907, 2732, 3272
BslFI GGGAC 4 cut(s) 1756, 3207, 3396, 3848
BslI CCNNNNNNNGG 4 cut(s) 1447, 1562, 1700, 4025
BsmAI GTCTC 8 cut(s) 1185, 1563, 1874, 2204, 2472, 2795, 3264, 3272
BsmBI CGTCTC 3 cut(s) 1185, 1563, 2472
BsmFI GGGAC 4 cut(s) 1756, 3207, 3396, 3848
BsmI GAATGC 3 cut(s) 1076, 3117, 3678
BsnI GGCC 4 cut(s) 2484, 2651, 3475, 4235
BsoBI CYCGRG 1 cut(s) 2264
Bsp119I TTCGAA 3 cut(s) 2747, 2879, 3219
Bsp1286I GDGCHC 2 cut(s) 840, 4288
Bsp13I TCCGGA 2 cut(s) 958, 1274
Bsp1407I TGTACA 3 cut(s) 391, 397, 1532
Bsp1720I GCTNAGC 1 cut(s) 4267
Bsp19I CCATGG 1 cut(s) 2949
BspANI GGCC 4 cut(s) 2484, 2651, 3475, 4235
BspCNI CTCAG 6 cut(s) 127, 298, 1446, 2423, 3243, 3562
BspEI TCCGGA 2 cut(s) 958, 1274
BspFNI CGCG 4 cut(s) 1265, 2681, 3520, 4195
BspHI TCATGA 2 cut(s) 2497, 4128
BspLI GGNNCC 3 cut(s) 606, 1744, 3543
BspMAI CTGCAG 1 cut(s) 3934
BspMI ACCTGC 2 cut(s) 1089, 3416
BspPI GGATC 8 cut(s) 218, 545, 599, 612, 1458, 1977, 2936, 4052
BspQI GCTCTTC 1 cut(s) 1074
BspT104I TTCGAA 3 cut(s) 2747, 2879, 3219
BspT107I GGYRCC 1 cut(s) 3541
BsrBI CCGCTC 1 cut(s) 3739
BsrDI GCAATG 2 cut(s) 570, 891
BsrGI TGTACA 3 cut(s) 391, 397, 1532
BsrI ACTGG 7 cut(s) 664, 1789, 3009, 3026, 3673, 3842, 4236
BssNAI GTATAC 1 cut(s) 595
BssNI GRCGYC 3 cut(s) 3385, 3542, 3747
BssT1I CCWWGG 4 cut(s) 696, 2949, 3126, 3919
Bst1107I GTATAC 1 cut(s) 595
Bst2UI CCWGG 8 cut(s) 308, 528, 816, 1086, 2978, 3243, 3638, 4237
Bst4CI ACNGT 8 cut(s) 956, 1814, 2004, 2377, 2914, 3203, 3266, 4088
Bst6I CTCTTC 4 cut(s) 1074, 1679, 3177, 3962
BstACI GRCGYC 3 cut(s) 3385, 3542, 3747
BstAUI TGTACA 3 cut(s) 391, 397, 1532
BstBAI YACGTR 2 cut(s) 443, 3146
BstBI TTCGAA 3 cut(s) 2747, 2879, 3219
BstC8I GCNNGC 5 cut(s) 1110, 1436, 1721, 2683, 3082
BstDEI CTNAG 9 cut(s) 135, 184, 285, 1454, 2410, 2925, 3230, 3549, 4267
BstDSI CCRYGG 4 cut(s) 2473, 2949, 3518, 3788
BstFNI CGCG 4 cut(s) 1265, 2681, 3520, 4195
BstH2I RGCGCY 2 cut(s) 2081, 3545
BstHHI GCGC 4 cut(s) 2080, 2687, 3544, 4197
BstMAI GTCTC 8 cut(s) 1185, 1563, 1874, 2204, 2472, 2795, 3264, 3272
BstMWI GCNNNNNNNGC 7 cut(s) 1154, 2257, 2269, 2684, 3359, 3472, 4041
BstNI CCWGG 8 cut(s) 308, 528, 816, 1086, 2978, 3243, 3638, 4237
BstNSI RCATGY 7 cut(s) 398, 900, 1387, 1438, 2573, 3618, 3993
BstPAI GACNNNNGTC 2 cut(s) 2375, 3849
BstSCI CCNGG 9 cut(s) 306, 526, 814, 1084, 2976, 3241, 3270, 3636, 4235
BstSFI CTRYAG 3 cut(s) 2000, 3323, 3930
BstSLI GKGCMC 1 cut(s) 4288
BstSNI TACGTA 1 cut(s) 443
BstUI CGCG 4 cut(s) 1265, 2681, 3520, 4195
BstV1I GCAGC 4 cut(s) 2067, 2247, 3930, 4047
BstV2I GAAGAC 3 cut(s) 251, 2276, 3138
BstX2I RGATCY 5 cut(s) 604, 989, 1450, 1504, 1969
BstXI CCANNNNNNTGG 3 cut(s) 412, 2966, 3148
BstYI RGATCY 5 cut(s) 604, 989, 1450, 1504, 1969
BstZ17I GTATAC 1 cut(s) 595
BsuRI GGCC 4 cut(s) 2484, 2651, 3475, 4235
BtgI CCRYGG 4 cut(s) 2473, 2949, 3518, 3788
BtsI GCAGTG 5 cut(s) 1632, 2601, 2709, 3303, 4278
BveI ACCTGC 2 cut(s) 1089, 3416
Cac8I GCNNGC 5 cut(s) 1110, 1436, 1721, 2683, 3082
CaiI CAGNNNCTG 1 cut(s) 2227
CciI TCATGA 2 cut(s) 2497, 4128
CfoI GCGC 4 cut(s) 2080, 2687, 3544, 4197
Cfr13I GGNCC 5 cut(s) 524, 956, 1743, 2956, 4052
Cfr42I CCGCGG 1 cut(s) 3521
CpoI CGGWCCG 1 cut(s) 956
CseI GACGC 1 cut(s) 3755
CspCI CAANNNNNGTGG 4 cut(s) 1524, 1559, 2276, 2311
CspI CGGWCCG 1 cut(s) 956
DdeI CTNAG 9 cut(s) 135, 184, 285, 1454, 2410, 2925, 3230, 3549, 4267
DinI GGCGCC 1 cut(s) 3543
DrdI GACNNNNNNGTC 1 cut(s) 3201
DseDI GACNNNNNNGTC 1 cut(s) 3201
EaeI YGGCCR 3 cut(s) 2482, 2649, 4233
Eam1104I CTCTTC 4 cut(s) 1074, 1679, 3177, 3962
EarI CTCTTC 4 cut(s) 1074, 1679, 3177, 3962
EciI GGCGGA 1 cut(s) 1614
Eco105I TACGTA 1 cut(s) 443
Eco130I CCWWGG 4 cut(s) 696, 2949, 3126, 3919
Eco32I GATATC 2 cut(s) 1257, 4126
Eco47I GGWCC 5 cut(s) 524, 956, 1743, 2956, 4052
Eco57I CTGAAG 5 cut(s) 614, 642, 1592, 1703, 2379
Eco72I CACGTG 1 cut(s) 3146
Eco88I CYCGRG 1 cut(s) 2264
EcoRI GAATTC 2 cut(s) 2350, 2555
EcoRII CCWGG 8 cut(s) 306, 526, 814, 1084, 2976, 3241, 3636, 4235
EcoRV GATATC 2 cut(s) 1257, 4126
EcoT14I CCWWGG 4 cut(s) 696, 2949, 3126, 3919
EcoT22I ATGCAT 2 cut(s) 1029, 3119
EgeI GGCGCC 1 cut(s) 3543
EheI GGCGCC 1 cut(s) 3543
ErhI CCWWGG 4 cut(s) 696, 2949, 3126, 3919
Esp3I CGTCTC 3 cut(s) 1185, 1563, 2472
FalI AAGNNNNNCTT 4 cut(s) 689, 721, 3681, 3713
FaqI GGGAC 4 cut(s) 1756, 3207, 3396, 3848
FauI CCCGC 2 cut(s) 3513, 3732
FbaI TGATCA 1 cut(s) 2494
FblI GTMKAC 3 cut(s) 594, 1478, 4294
Fnu4HI GCNGC 9 cut(s) 1396, 2081, 2261, 2679, 3294, 3540, 3944, 3987, 4036
Fsp4HI GCNGC 9 cut(s) 1396, 2081, 2261, 2679, 3294, 3540, 3944, 3987, 4036
FspBI CTAG 4 cut(s) 1640, 2033, 2084, 4257
GlaI GCGC 4 cut(s) 2079, 2686, 3543, 4196
GluI GCNGC 9 cut(s) 1396, 2081, 2261, 2679, 3294, 3540, 3944, 3987, 4036
GsuI CTGGAG 8 cut(s) 329, 1467, 1745, 2204, 2595, 3656, 3740, 4237
HaeII RGCGCY 2 cut(s) 2081, 3545
HaeIII GGCC 4 cut(s) 2484, 2651, 3475, 4235
HapII CCGG 7 cut(s) 959, 1275, 1361, 1441, 1907, 2732, 3272
HgaI GACGC 1 cut(s) 3755
HhaI GCGC 4 cut(s) 2080, 2687, 3544, 4197
Hin1I GRCGYC 3 cut(s) 3385, 3542, 3747
Hin6I GCGC 4 cut(s) 2078, 2685, 3542, 4195
HinP1I GCGC 4 cut(s) 2078, 2685, 3542, 4195
HincII GTYRAC 1 cut(s) 1479
HindII GTYRAC 1 cut(s) 1479
HindIII AAGCTT 5 cut(s) 1537, 1717, 1940, 2270, 3702
HpaII CCGG 7 cut(s) 959, 1275, 1361, 1441, 1907, 2732, 3272
HphI GGTGA 7 cut(s) 152, 308, 1455, 1544, 2462, 3440, 3890
Hpy166II GTNNAC 7 cut(s) 595, 1204, 1381, 1479, 3047, 3634, 4295
Hpy8I GTNNAC 7 cut(s) 595, 1204, 1381, 1479, 3047, 3634, 4295
Hpy99I CGWCG 2 cut(s) 986, 1153
HpyCH4III ACNGT 8 cut(s) 956, 1814, 2004, 2377, 2914, 3203, 3266, 4088
HpyF10VI GCNNNNNNNGC 7 cut(s) 1154, 2257, 2269, 2684, 3359, 3472, 4041
HpyF3I CTNAG 9 cut(s) 135, 184, 285, 1454, 2410, 2925, 3230, 3549, 4267
Hsp92I GRCGYC 3 cut(s) 3385, 3542, 3747
HspAI GCGC 4 cut(s) 2078, 2685, 3542, 4195
KasI GGCGCC 1 cut(s) 3541
Kpn2I TCCGGA 2 cut(s) 958, 1274
Ksp22I TGATCA 1 cut(s) 2494
KspI CCGCGG 1 cut(s) 3521
LguI GCTCTTC 1 cut(s) 1074
LmnI GCTCC 6 cut(s) 1431, 1443, 1726, 1892, 2223, 2519
Lsp1109I GCAGC 4 cut(s) 2067, 2247, 3930, 4047
MaeI CTAG 4 cut(s) 1640, 2033, 2084, 4257
MbiI CCGCTC 1 cut(s) 3739
MfeI CAATTG 3 cut(s) 491, 2255, 3303
MflI RGATCY 5 cut(s) 604, 989, 1450, 1504, 1969
MhlI GDGCHC 2 cut(s) 840, 4288
MlsI TGGCCA 1 cut(s) 4235
MluNI TGGCCA 1 cut(s) 4235
Mly113I GGCGCC 1 cut(s) 3542
MlyI GAGTC 8 cut(s) 149, 1474, 2218, 2369, 3189, 3411, 3594, 4088
MmeI TCCRAC 4 cut(s) 1208, 2787, 3394, 4029
Mox20I TGGCCA 1 cut(s) 4235
Mph1103I ATGCAT 2 cut(s) 1029, 3119
MroI TCCGGA 2 cut(s) 958, 1274
MscI TGGCCA 1 cut(s) 4235
MslI CAYNNNNRTG 3 cut(s) 165, 2701, 3146
Msp20I TGGCCA 1 cut(s) 4235
MspA1I CMGCKG 2 cut(s) 3520, 3943
MspI CCGG 7 cut(s) 959, 1275, 1361, 1441, 1907, 2732, 3272
MspR9I CCNGG 9 cut(s) 308, 528, 816, 1086, 2978, 3243, 3272, 3638, 4237
MunI CAATTG 3 cut(s) 491, 2255, 3303
Mva1269I GAATGC 3 cut(s) 1076, 3117, 3678
MvaI CCWGG 8 cut(s) 308, 528, 816, 1086, 2978, 3243, 3638, 4237
MvnI CGCG 4 cut(s) 1265, 2681, 3520, 4195
MwoI GCNNNNNNNGC 7 cut(s) 1154, 2257, 2269, 2684, 3359, 3472, 4041
NarI GGCGCC 1 cut(s) 3542
NciI CCSGG 1 cut(s) 3272
NcoI CCATGG 1 cut(s) 2949
NlaIV GGNNCC 3 cut(s) 606, 1744, 3543
NmeAIII GCCGAG 2 cut(s) 2042, 2528
NmuCI GTSAC 6 cut(s) 296, 950, 2313, 2368, 3428, 3842
NsiI ATGCAT 2 cut(s) 1029, 3119
NspI RCATGY 7 cut(s) 398, 900, 1387, 1438, 2573, 3618, 3993
NspV TTCGAA 3 cut(s) 2747, 2879, 3219
OliI CACNNNNGTG 1 cut(s) 3146
PaeI GCATGC 1 cut(s) 1438
PagI TCATGA 2 cut(s) 2497, 4128
PaqCI CACCTGC 1 cut(s) 3416
PasI CCCWGGG 1 cut(s) 3242
PciI ACATGT 2 cut(s) 394, 3614
PciSI GCTCTTC 1 cut(s) 1074
PcsI WCGNNNNNNNCGW 2 cut(s) 2753, 3151
PctI GAATGC 3 cut(s) 1076, 3117, 3678
PflMI CCANNNNNTGG 2 cut(s) 1700, 4025
PfoI TCCNGGA 3 cut(s) 306, 814, 3270
PkrI GCNGC 9 cut(s) 1397, 2082, 2262, 2680, 3295, 3541, 3945, 3988, 4037
PleI GAGTC 8 cut(s) 149, 1474, 2218, 2368, 3189, 3411, 3593, 4088
PluTI GGCGCC 1 cut(s) 3545
PmaCI CACGTG 1 cut(s) 3146
PmlI CACGTG 1 cut(s) 3146
PpsI GAGTC 8 cut(s) 149, 1474, 2218, 2368, 3189, 3411, 3593, 4088
Ppu21I YACGTR 2 cut(s) 443, 3146
PscI ACATGT 2 cut(s) 394, 3614
PshAI GACNNNNGTC 2 cut(s) 2375, 3849
Psp1406I AACGTT 1 cut(s) 3162
Psp6I CCWGG 8 cut(s) 306, 526, 814, 1084, 2976, 3241, 3636, 4235
PspCI CACGTG 1 cut(s) 3146
PspGI CCWGG 8 cut(s) 306, 526, 814, 1084, 2976, 3241, 3636, 4235
PspN4I GGNNCC 3 cut(s) 606, 1744, 3543
PspPI GGNCC 5 cut(s) 524, 956, 1743, 2956, 4052
PstI CTGCAG 1 cut(s) 3934
PstNI CAGNNNCTG 1 cut(s) 2227
PsuI RGATCY 5 cut(s) 604, 989, 1450, 1504, 1969
PvuII CAGCTG 1 cut(s) 3943
RseI CAYNNNNRTG 3 cut(s) 165, 2701, 3146
Rsr2I CGGWCCG 1 cut(s) 956
RsrII CGGWCCG 1 cut(s) 956
SacII CCGCGG 1 cut(s) 3521
SalI GTCGAC 1 cut(s) 1477
SapI GCTCTTC 1 cut(s) 1074
SatI GCNGC 9 cut(s) 1396, 2081, 2261, 2679, 3294, 3540, 3944, 3987, 4036
Sau96I GGNCC 5 cut(s) 524, 956, 1743, 2956, 4052
ScaI AGTACT 1 cut(s) 3800
SchI GAGTC 8 cut(s) 149, 1474, 2218, 2369, 3189, 3411, 3594, 4088
ScrFI CCNGG 9 cut(s) 308, 528, 816, 1086, 2978, 3243, 3272, 3638, 4237
SduI GDGCHC 2 cut(s) 840, 4288
SfcI CTRYAG 3 cut(s) 2000, 3323, 3930
SfoI GGCGCC 1 cut(s) 3543
Sfr303I CCGCGG 1 cut(s) 3521
SfuI TTCGAA 3 cut(s) 2747, 2879, 3219
SgrBI CCGCGG 1 cut(s) 3521
SinI GGWCC 5 cut(s) 524, 956, 1743, 2956, 4052
SmiMI CAYNNNNRTG 3 cut(s) 165, 2701, 3146
SmlI CTYRAG 5 cut(s) 686, 1250, 1313, 2089, 3188
SmoI CTYRAG 5 cut(s) 686, 1250, 1313, 2089, 3188
SnaBI TACGTA 1 cut(s) 443
SphI GCATGC 1 cut(s) 1438
SspDI GGCGCC 1 cut(s) 3541
SspI AATATT 2 cut(s) 45, 1656
SspMI CTAG 4 cut(s) 1640, 2033, 2084, 4257
StyD4I CCNGG 9 cut(s) 306, 526, 814, 1084, 2976, 3241, 3270, 3636, 4235
StyI CCWWGG 4 cut(s) 696, 2949, 3126, 3919
TaaI ACNGT 8 cut(s) 956, 1814, 2004, 2377, 2914, 3203, 3266, 4088
TaqII GACCGA 1 cut(s) 3195
TatI WGTACW 7 cut(s) 197, 391, 397, 788, 1129, 1532, 3798
TauI GCSGC 5 cut(s) 1398, 2681, 3296, 3542, 3989
TseFI GTSAC 6 cut(s) 296, 950, 2313, 2368, 3428, 3842
TseI GCWGC 4 cut(s) 2080, 2260, 3943, 4035
Tsp45I GTSAC 6 cut(s) 296, 950, 2313, 2368, 3428, 3842
TspGWI ACGGA 5 cut(s) 2265, 2336, 2490, 3447, 3614
Van91I CCANNNNNTGG 2 cut(s) 1700, 4025
VpaK11BI GGWCC 5 cut(s) 524, 956, 1743, 2956, 4052
XceI RCATGY 7 cut(s) 398, 900, 1387, 1438, 2573, 3618, 3993
XcmI CCANNNNNNNNNTGG 1 cut(s) 3102
XmiI GTMKAC 3 cut(s) 594, 1478, 4294
XspI CTAG 4 cut(s) 1640, 2033, 2084, 4257
ZraI GACGTC 1 cut(s) 3386
ZrmI AGTACT 1 cut(s) 3800
Zsp2I ATGCAT 2 cut(s) 1029, 3119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.