Rh6BG254000

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000129
Physical Location & Seq
Reverse (-)
46619505 .. 46622072
2568 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG254000.1

Sequence Viewer

Length: 2130 bp
ATGAGTTCCTGCCAGGAAGGGGAATGGAAGCCAGCTACTGGGATTTCAATTGAAAGACAAGTACTTCGAAAACAGCTCGAGTTATTTGATTTTAGGTCATCAGACAGTAAGATACATGCAGTTGGTCGTGATCAAACTGTAATTCCAGAGTGGTTCAACATCATAGCTAGCAGATGTTCCATAGGACACCCGTTGATTCCAAATTTAAAAGATGATAAGCAGTGGATGGGGGTTGCCGTGTGTGCTATTTTCTCAGTCAAGGAATTACAACATCCTCCTGTCTCCGGCATTGAATTTGATCCAGAAACTTCTAACTACTTCTACCGGTGCACAGTGGGAACAGCTGAATTTGATCTAGAACCATGTATCCTGTACGACGAGCTACATCGCCATGTTGTTACATCTGGGTCTACTTTTGGTTGTAGTTTTTACGTCCCTTGGGTGCACTTTCCGGAGATGTTAAATGAATCATTTCTAATGGTGGCTTTTTTTGACACCAACAACCCACTCATGGAGGTCAAGAAATGTGGGATTCGTTTATTATATGAGCAAGACATTGATGGGTTTATTGAAACAATATTGCAGTTGGAGTTAGATGGTGGCCGTCAACATCTAATCCCTAATTTGCTTACTACCGGAGAGAAACCTGAAATTCCTTCCAACACCTGCGAATTAAATGAAGTTGATAAAGCTCTGTTTGAGTCGGGATGGTTTCACCTAGTAGACGATAATATTTTGAGATGGGAGAAAGTTATTCCAATTCGCGAAGAAGGTTCCATCATTATGCTAAGAAAGAACATCGAATCTGTTCTTCCAAGATACCTGGAGGGACTAAATAACGAGACGTTTCAATACTATCATTTTGATCTAAGTGGAAGCCCAGCATGGTTCAGTCCTCAAATTGGGTTCACCGCGACAATGGACCTGCGTGATAATCTACAAAAGAGTAAGAAGTGGATGGGATTTGCTCTATGCGCTTCACTTACAGTGAAGGAGGAGGATTACAGTGGTTCTTGTGGACTTCAAATTGGTATATTTTTTTTAAGGGAAGGCGGCATTTCTGGAAAGCTATTTTCCACCCAAAAGATATCTCATGGACTCGTCCTCATTTACATACCGAAAGCAAAATTTTTGGAAGATGAATTCTCAGAAATAGGTTCAGGTAGCAAGATTTGCATTGTTTGTAAAAGCGATAACCCTGATATCGAAGTTAAAACCTGTGGGTTCCGTATTTTGTACCAGCAAGATTTACAAGGTTTTGTTCAGAGAATTACCCACTGCATATTGCGTAGTTCAGATTTCGACCATTATAATAAATTGGTGGTAGAAGATTGGATAAGTTTGATAGGATTGCAAAGCCCTAAAGTCCATTTCGAGAAAACGATACAAGAAGCTACTCCCACCGGGACGGTCTCAAGTCAACAGAATGCAGAGAGCGTTGAAGAACTCCCTATTCGGCGACCAGAAGTTAAGGCTTTTCAGTTTTCTCAGCAACTTAGACATCAGGACTGGACTGTGTTTGGGCTTAACAGCCGTTTCCCTGGAATCAATATTCCCAAGTGGTTCACGAATTTGAACAAAGGCAGCTCTGCAGAAATCTCACTCCCTCCAAATTTGTTCGATGATGGTAACTGGTTGGGGATTTCTGTTTGCACTTATGTCTCAGTCCAGCAACATCCAACCAATATTATTGACATTAGTAATCCAGATTCTCAGAATAGTCGTGAGCTTATTTGTTATCTGGATTATACCGATGTTGTTAGGGTCATCTCTCTCCCTTTCCCAGCTGAGGCAGGCCGTGGCATGTGGTCGCATCCATGCGATTTTAGTTGGTTCATCTACATACCGCGAGTTCGGTTTTCTAGTTTCTTGAACGAATGCAATGCAGCCACAGCAACCATTGGGTCCAATAGCCTTGACTTGGGACTACAAGAATGTGCACTACGTCTTGTATTTAAGGAGGATGTAGAAGATCTTATACAGACCCTGTCCCTCTGTCACTCATCTTCAAGATGCCATCACTGTGAGCACGCTGCAGCTGGTAGTCATGATTCAAGAGATTTGGCTTCTGCATCTATGGACTCTACTGCAAGGGTATCACATGTTGGGCCATCCAATTCCAACCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

709

Amino Acids

80.14

Weight (kDa)

5.2

Isoelectric Point (pI)

50.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C-JID PF20160 46 - 186 2.2e-06 C-JID domain
C-JID PF20160 513 - 657 1.2e-09 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1311
AarI CACCTGC 1 cut(s) 674
Acc36I ACCTGC 2 cut(s) 674, 933
AccB7I CCANNNNNTGG 1 cut(s) 38
AccI GTMKAC 2 cut(s) 410, 723
AccII CGCG 3 cut(s) 765, 914, 1849
AccIII TCCGGA 1 cut(s) 451
AciI CCGC 3 cut(s) 912, 1053, 1847
AclWI GGATC 1 cut(s) 293
AcoI YGGCCR 1 cut(s) 601
AcsI RAATTY 8 cut(s) 202, 293, 347, 651, 1127, 1142, 1570, 1612
AfaI GTAC 3 cut(s) 63, 374, 1238
AfiI CCNNNNNNNGG 7 cut(s) 19, 38, 284, 511, 902, 1789, 1921
AflIII ACRYGT 1 cut(s) 2101
AgeI ACCGGT 1 cut(s) 324
AjnI CCWGG 3 cut(s) 12, 822, 1540
AloI GAACNNNNNNTCC 2 cut(s) 351, 383
Alw21I GWGCWC 4 cut(s) 332, 447, 1942, 2031
Alw26I GTCTC 4 cut(s) 286, 836, 1417, 1666
Alw44I GTGCAC 3 cut(s) 328, 443, 1938
AlwI GGATC 1 cut(s) 293
AlwNI CAGNNNCTG 2 cut(s) 38, 1987
Ama87I CYCGRG 1 cut(s) 77
Aor13HI TCCGGA 1 cut(s) 451
AoxI GGCC 3 cut(s) 601, 1795, 2108
ApaLI GTGCAC 3 cut(s) 328, 443, 1938
ApeKI GCWGC 4 cut(s) 1584, 1886, 2033, 2036
ApoI RAATTY 8 cut(s) 202, 293, 347, 651, 1127, 1142, 1570, 1612
ArsI GACNNNNNNTTYG 2 cut(s) 48, 80
AsiGI ACCGGT 1 cut(s) 324
Asp700I GAANNNNTTC 2 cut(s) 471, 807
AspLEI GCGC 1 cut(s) 977
AspS9I GGNCC 3 cut(s) 922, 1905, 2108
AsuC2I CCSGG 1 cut(s) 1405
AsuHPI GGTGA 2 cut(s) 707, 901
AsuII TTCGAA 1 cut(s) 67
AsuNHI GCTAGC 1 cut(s) 167
AvaI CYCGRG 1 cut(s) 77
AvaII GGWCC 2 cut(s) 922, 1905
BaeGI GKGCMC 3 cut(s) 332, 447, 1942
BarI GAAGNNNNNNTAC 2 cut(s) 1962, 1994
Bbv12I GWGCWC 4 cut(s) 332, 447, 1942, 2031
BbvCI CCTCAGC 1 cut(s) 1788
BbvI GCAGC 4 cut(s) 1596, 1898, 2020, 2048
BceAI ACGGC 4 cut(s) 221, 588, 1518, 1782
BcgI CGANNNNNNTGC 2 cut(s) 1865, 1899
BciT130I CCWGG 3 cut(s) 14, 824, 1542
BciVI GTATCC 1 cut(s) 377
BclI TGATCA 1 cut(s) 130
BcnI CCSGG 1 cut(s) 1405
BcoDI GTCTC 4 cut(s) 286, 836, 1417, 1666
BfaI CTAG 4 cut(s) 168, 356, 719, 1863
BfmI CTRYAG 2 cut(s) 1590, 2034
BfuAI ACCTGC 2 cut(s) 674, 933
BfuI GTATCC 1 cut(s) 377
BglII AGATCT 1 cut(s) 1972
BisI GCNGC 5 cut(s) 1054, 1585, 1887, 2034, 2037
BlsI GCNGC 5 cut(s) 1055, 1586, 1888, 2035, 2038
BmcAI AGTACT 1 cut(s) 63
Bme1390I CCNGG 4 cut(s) 14, 824, 1405, 1542
Bme18I GGWCC 2 cut(s) 922, 1905
BmeT110I CYCGRG 1 cut(s) 77
BmgT120I GGNCC 3 cut(s) 922, 1905, 2108
BmiI GGNNCC 3 cut(s) 775, 1226, 1906
BmrFI CCNGG 4 cut(s) 14, 824, 1405, 1542
BmrI ACTGGG 1 cut(s) 48
BmsI GCATC 3 cut(s) 1822, 2003, 2081
BmtI GCTAGC 1 cut(s) 171
BmuI ACTGGG 1 cut(s) 48
BpmI CTGGAG 1 cut(s) 845
Bpu10I CCTNAGC 1 cut(s) 1788
Bpu14I TTCGAA 1 cut(s) 67
BpuEI CTTGAG 1 cut(s) 1399
BpuMI CCSGG 1 cut(s) 1405
BsaI GGTCTC 1 cut(s) 1417
BsaJI CCNNGG 3 cut(s) 437, 1540, 1798
BsaWI WCCGGW 3 cut(s) 324, 451, 635
Bsc4I CCNNNNNNNGG 7 cut(s) 19, 38, 284, 511, 902, 1789, 1921
Bse118I RCCGGY 1 cut(s) 324
Bse1I ACTGG 3 cut(s) 43, 1514, 1637
Bse3DI GCAATG 1 cut(s) 1888
BseAI TCCGGA 1 cut(s) 451
BseBI CCWGG 3 cut(s) 14, 824, 1542
BseDI CCNNGG 3 cut(s) 437, 1540, 1798
BseGI GGATG 8 cut(s) 231, 271, 713, 963, 1675, 1813, 1969, 2111
BseLI CCNNNNNNNGG 7 cut(s) 19, 38, 284, 511, 902, 1789, 1921
BseMI GCAATG 1 cut(s) 1888
BseMII CTCAG 6 cut(s) 267, 1161, 1502, 1677, 1727, 1779
BseNI ACTGG 3 cut(s) 43, 1514, 1637
BseRI GAGGAG 1 cut(s) 1010
BseSI GKGCMC 3 cut(s) 332, 447, 1942
BseXI GCAGC 4 cut(s) 1596, 1898, 2020, 2048
BseYI CCCAGC 2 cut(s) 880, 1783
Bsh1236I CGCG 3 cut(s) 765, 914, 1849
BshFI GGCC 3 cut(s) 603, 1797, 2110
BshTI ACCGGT 1 cut(s) 324
BsiHKAI GWGCWC 4 cut(s) 332, 447, 1942, 2031
BsiHKCI CYCGRG 1 cut(s) 77
BsiSI CCGG 5 cut(s) 285, 325, 452, 636, 1404
BslFI GGGAC 5 cut(s) 419, 843, 1420, 1938, 1975
BslI CCNNNNNNNGG 7 cut(s) 19, 38, 284, 511, 902, 1789, 1921
BsmAI GTCTC 4 cut(s) 286, 836, 1417, 1666
BsmBI CGTCTC 1 cut(s) 836
BsmFI GGGAC 5 cut(s) 419, 843, 1420, 1938, 1975
BsmI GAATGC 2 cut(s) 1432, 1883
BsnI GGCC 3 cut(s) 603, 1797, 2110
Bso31I GGTCTC 1 cut(s) 1417
BsoBI CYCGRG 1 cut(s) 77
Bsp119I TTCGAA 1 cut(s) 67
Bsp1286I GDGCHC 4 cut(s) 332, 447, 1942, 2031
Bsp13I TCCGGA 1 cut(s) 451
Bsp143I GATC 5 cut(s) 130, 298, 352, 865, 1972
Bsp68I TCGCGA 1 cut(s) 765
BspACI CCGC 3 cut(s) 912, 1053, 1847
BspANI GGCC 3 cut(s) 603, 1797, 2110
BspCNI CTCAG 6 cut(s) 266, 1160, 1501, 1676, 1726, 1780
BspEI TCCGGA 1 cut(s) 451
BspFNI CGCG 3 cut(s) 765, 914, 1849
BspHI TCATGA 1 cut(s) 2047
BspLI GGNNCC 3 cut(s) 775, 1226, 1906
BspMAI CTGCAG 2 cut(s) 1594, 2038
BspMI ACCTGC 2 cut(s) 674, 933
BspOI GCTAGC 1 cut(s) 171
BspPI GGATC 1 cut(s) 293
BspT104I TTCGAA 1 cut(s) 67
BspTNI GGTCTC 1 cut(s) 1417
BsrDI GCAATG 1 cut(s) 1888
BsrFI RCCGGY 1 cut(s) 324
BsrI ACTGG 3 cut(s) 43, 1514, 1637
BssAI RCCGGY 1 cut(s) 324
BssECI CCNNGG 3 cut(s) 437, 1540, 1798
BssMI GATC 5 cut(s) 130, 298, 352, 865, 1972
BssT1I CCWWGG 1 cut(s) 437
Bst2UI CCWGG 3 cut(s) 14, 824, 1542
Bst4CI ACNGT 8 cut(s) 107, 139, 334, 988, 1007, 1411, 1516, 2024
BstAPI GCANNNNNTGC 1 cut(s) 1173
BstBI TTCGAA 1 cut(s) 67
BstC8I GCNNGC 4 cut(s) 33, 169, 1795, 2031
BstDEI CTNAG 9 cut(s) 253, 788, 869, 1147, 1488, 1496, 1663, 1713, 1788
BstDSI CCRYGG 1 cut(s) 1798
BstF5I GGATG 8 cut(s) 231, 271, 713, 963, 1675, 1813, 1969, 2111
BstFNI CGCG 3 cut(s) 765, 914, 1849
BstHHI GCGC 1 cut(s) 977
BstKTI GATC 5 cut(s) 133, 301, 355, 868, 1975
BstMAI GTCTC 4 cut(s) 286, 836, 1417, 1666
BstMBI GATC 5 cut(s) 130, 298, 352, 865, 1972
BstMWI GCNNNNNNNGC 4 cut(s) 242, 974, 1173, 1892
BstNI CCWGG 3 cut(s) 14, 824, 1542
BstNSI RCATGY 3 cut(s) 119, 1807, 2105
BstSCI CCNGG 4 cut(s) 12, 822, 1403, 1540
BstSFI CTRYAG 2 cut(s) 1590, 2034
BstSLI GKGCMC 3 cut(s) 332, 447, 1942
BstUI CGCG 3 cut(s) 765, 914, 1849
BstV1I GCAGC 4 cut(s) 1596, 1898, 2020, 2048
BstX2I RGATCY 1 cut(s) 1972
BstYI RGATCY 1 cut(s) 1972
BsuI GTATCC 1 cut(s) 377
BsuRI GGCC 3 cut(s) 603, 1797, 2110
BtgI CCRYGG 1 cut(s) 1798
BtgZI GCGATG 1 cut(s) 371
BtsCI GGATG 8 cut(s) 231, 271, 713, 963, 1675, 1813, 1969, 2111
BtsI GCAGTG 2 cut(s) 227, 1276
BtsIMutI CAGTG 6 cut(s) 227, 339, 993, 1012, 1276, 2020
BtuMI TCGCGA 1 cut(s) 765
BveI ACCTGC 2 cut(s) 674, 933
Cac8I GCNNGC 4 cut(s) 33, 169, 1795, 2031
CaiI CAGNNNCTG 2 cut(s) 38, 1987
CciI TCATGA 1 cut(s) 2047
CfoI GCGC 1 cut(s) 977
Cfr10I RCCGGY 1 cut(s) 324
Cfr13I GGNCC 3 cut(s) 922, 1905, 2108
Csp6I GTAC 3 cut(s) 62, 373, 1237
CspAI ACCGGT 1 cut(s) 324
CspCI CAANNNNNGTGG 2 cut(s) 508, 543
CviQI GTAC 3 cut(s) 62, 373, 1237
DdeI CTNAG 9 cut(s) 253, 788, 869, 1147, 1488, 1496, 1663, 1713, 1788
DpnI GATC 5 cut(s) 132, 300, 354, 867, 1974
DpnII GATC 5 cut(s) 130, 298, 352, 865, 1972
DraI TTTAAA 1 cut(s) 207
EaeI YGGCCR 1 cut(s) 601
Eco130I CCWWGG 1 cut(s) 437
Eco31I GGTCTC 1 cut(s) 1417
Eco32I GATATC 2 cut(s) 1089, 1204
Eco47I GGWCC 2 cut(s) 922, 1905
Eco88I CYCGRG 1 cut(s) 77
EcoRI GAATTC 1 cut(s) 1142
EcoRII CCWGG 3 cut(s) 12, 822, 1540
EcoRV GATATC 2 cut(s) 1089, 1204
EcoT14I CCWWGG 1 cut(s) 437
ErhI CCWWGG 1 cut(s) 437
Esp3I CGTCTC 1 cut(s) 836
FaqI GGGAC 5 cut(s) 419, 843, 1420, 1938, 1975
FbaI TGATCA 1 cut(s) 130
FblI GTMKAC 2 cut(s) 410, 723
Fnu4HI GCNGC 5 cut(s) 1054, 1585, 1887, 2034, 2037
FokI GGATG 8 cut(s) 238, 258, 720, 970, 1662, 1800, 1976, 2098
Fsp4HI GCNGC 5 cut(s) 1054, 1585, 1887, 2034, 2037
FspBI CTAG 4 cut(s) 168, 356, 719, 1863
GlaI GCGC 1 cut(s) 976
GluI GCNGC 5 cut(s) 1054, 1585, 1887, 2034, 2037
GsaI CCCAGC 2 cut(s) 884, 1787
GsuI CTGGAG 1 cut(s) 845
HaeIII GGCC 3 cut(s) 603, 1797, 2110
HapII CCGG 5 cut(s) 285, 325, 452, 636, 1404
HhaI GCGC 1 cut(s) 977
Hin6I GCGC 1 cut(s) 975
HinP1I GCGC 1 cut(s) 975
HincII GTYRAC 2 cut(s) 608, 1421
HindII GTYRAC 2 cut(s) 608, 1421
HpaII CCGG 5 cut(s) 285, 325, 452, 636, 1404
HphI GGTGA 2 cut(s) 707, 901
Hpy188I TCNGA 5 cut(s) 103, 1150, 1266, 1297, 1716
Hpy99I CGWCG 1 cut(s) 380
HpyAV CCTTC 5 cut(s) 11, 666, 764, 985, 1043
HpyCH4III ACNGT 8 cut(s) 107, 139, 334, 988, 1007, 1411, 1516, 2024
HpyCH4IV ACGT 3 cut(s) 432, 845, 1945
HpyF10VI GCNNNNNNNGC 4 cut(s) 242, 974, 1173, 1892
HpyF3I CTNAG 9 cut(s) 253, 788, 869, 1147, 1488, 1496, 1663, 1713, 1788
HpySE526I ACGT 3 cut(s) 432, 845, 1945
HspAI GCGC 1 cut(s) 975
Kpn2I TCCGGA 1 cut(s) 451
Ksp22I TGATCA 1 cut(s) 130
Kzo9I GATC 5 cut(s) 130, 298, 352, 865, 1972
Lsp1109I GCAGC 4 cut(s) 1596, 1898, 2020, 2048
LweI GCATC 3 cut(s) 1822, 2003, 2081
MaeI CTAG 4 cut(s) 168, 356, 719, 1863
MaeII ACGT 3 cut(s) 432, 845, 1945
MaeIII GTNAC 3 cut(s) 397, 1628, 1997
MalI GATC 5 cut(s) 132, 300, 354, 867, 1974
MboI GATC 5 cut(s) 130, 298, 352, 865, 1972
MboII GAAGA 7 cut(s) 779, 803, 1148, 1340, 1454, 1982, 1998
MfeI CAATTG 1 cut(s) 48
MflI RGATCY 1 cut(s) 1972
MhlI GDGCHC 4 cut(s) 332, 447, 1942, 2031
MlyI GAGTC 3 cut(s) 710, 1092, 2075
MmeI TCCRAC 3 cut(s) 567, 684, 1703
MroI TCCGGA 1 cut(s) 451
MroXI GAANNNNTTC 2 cut(s) 471, 807
MseI TTAA 8 cut(s) 206, 461, 674, 1043, 1212, 1470, 1527, 1956
MslI CAYNNNNRTG 3 cut(s) 390, 782, 2022
MspA1I CMGCKG 3 cut(s) 344, 1787, 2039
MspI CCGG 5 cut(s) 285, 325, 452, 636, 1404
MspR9I CCNGG 4 cut(s) 14, 824, 1405, 1542
MunI CAATTG 1 cut(s) 48
Mva1269I GAATGC 2 cut(s) 1432, 1883
MvaI CCWGG 3 cut(s) 14, 824, 1542
MvnI CGCG 3 cut(s) 765, 914, 1849
MwoI GCNNNNNNNGC 4 cut(s) 242, 974, 1173, 1892
NciI CCSGG 1 cut(s) 1405
NdeII GATC 5 cut(s) 130, 298, 352, 865, 1972
NheI GCTAGC 1 cut(s) 167
NlaIV GGNNCC 3 cut(s) 775, 1226, 1906
NmuCI GTSAC 1 cut(s) 1997
NruI TCGCGA 1 cut(s) 765
NspI RCATGY 3 cut(s) 119, 1807, 2105
NspV TTCGAA 1 cut(s) 67
PaeR7I CTCGAG 1 cut(s) 77
PagI TCATGA 1 cut(s) 2047
PaqCI CACCTGC 1 cut(s) 674
PciI ACATGT 1 cut(s) 2101
PctI GAATGC 2 cut(s) 1432, 1883
PdmI GAANNNNTTC 2 cut(s) 471, 807
PfeI GAWTC 7 cut(s) 196, 467, 532, 803, 1545, 1709, 2051
PflFI GACNNNGTC 1 cut(s) 1987
PflMI CCANNNNNTGG 1 cut(s) 38
PinAI ACCGGT 1 cut(s) 324
PkrI GCNGC 5 cut(s) 1055, 1586, 1888, 2035, 2038
PleI GAGTC 3 cut(s) 709, 1092, 2075
PpsI GAGTC 3 cut(s) 709, 1092, 2075
PscI ACATGT 1 cut(s) 2101
PsiI TTATAA 1 cut(s) 1311
Psp6I CCWGG 3 cut(s) 12, 822, 1540
PspFI CCCAGC 2 cut(s) 880, 1783
PspGI CCWGG 3 cut(s) 12, 822, 1540
PspN4I GGNNCC 3 cut(s) 775, 1226, 1906
PspPI GGNCC 3 cut(s) 922, 1905, 2108
PspXI VCTCGAGB 1 cut(s) 77
PstI CTGCAG 2 cut(s) 1594, 2038
PstNI CAGNNNCTG 2 cut(s) 38, 1987
PsuI RGATCY 1 cut(s) 1972
PsyI GACNNNGTC 1 cut(s) 1987
PvuII CAGCTG 3 cut(s) 344, 1787, 2039
RruI TCGCGA 1 cut(s) 765
RsaI GTAC 3 cut(s) 63, 374, 1238
RsaNI GTAC 3 cut(s) 62, 373, 1237
RseI CAYNNNNRTG 3 cut(s) 390, 782, 2022
SaqAI TTAA 8 cut(s) 206, 461, 674, 1043, 1212, 1470, 1527, 1956
SatI GCNGC 5 cut(s) 1054, 1585, 1887, 2034, 2037
Sau3AI GATC 5 cut(s) 130, 298, 352, 865, 1972
Sau96I GGNCC 3 cut(s) 922, 1905, 2108
ScaI AGTACT 1 cut(s) 63
SchI GAGTC 3 cut(s) 710, 1092, 2075
ScrFI CCNGG 4 cut(s) 14, 824, 1405, 1542
SduI GDGCHC 4 cut(s) 332, 447, 1942, 2031
SfaNI GCATC 3 cut(s) 1822, 2003, 2081
SfcI CTRYAG 2 cut(s) 1590, 2034
Sfr274I CTCGAG 1 cut(s) 77
SfuI TTCGAA 1 cut(s) 67
SinI GGWCC 2 cut(s) 922, 1905
SlaI CTCGAG 1 cut(s) 77
SmiMI CAYNNNNRTG 3 cut(s) 390, 782, 2022
SmlI CTYRAG 2 cut(s) 77, 1414
SmoI CTYRAG 2 cut(s) 77, 1414
SsiI CCGC 3 cut(s) 912, 1053, 1847
SspI AATATT 4 cut(s) 579, 733, 1552, 1687
SspMI CTAG 4 cut(s) 168, 356, 719, 1863
StyD4I CCNGG 4 cut(s) 12, 822, 1403, 1540
StyI CCWWGG 1 cut(s) 437
TaaI ACNGT 8 cut(s) 107, 139, 334, 988, 1007, 1411, 1516, 2024
TaiI ACGT 3 cut(s) 435, 848, 1948
TaqI TCGA 7 cut(s) 67, 78, 801, 1206, 1302, 1374, 1620
TatI WGTACW 1 cut(s) 61
TauI GCSGC 1 cut(s) 1056
TfiI GAWTC 7 cut(s) 196, 467, 532, 803, 1545, 1709, 2051
Tru1I TTAA 8 cut(s) 206, 461, 674, 1043, 1212, 1470, 1527, 1956
Tru9I TTAA 8 cut(s) 206, 461, 674, 1043, 1212, 1470, 1527, 1956
TscAI CASTG 6 cut(s) 227, 339, 993, 1012, 1283, 2027
TseFI GTSAC 1 cut(s) 1997
TseI GCWGC 4 cut(s) 1584, 1886, 2033, 2036
Tsp45I GTSAC 1 cut(s) 1997
TspDTI ATGAA 4 cut(s) 480, 693, 1155, 1825
TspGWI ACGGA 1 cut(s) 1217
TspRI CASTG 6 cut(s) 227, 339, 993, 1012, 1283, 2027
Tth111I GACNNNGTC 1 cut(s) 1987
Van91I CCANNNNNTGG 1 cut(s) 38
VneI GTGCAC 3 cut(s) 328, 443, 1938
VpaK11BI GGWCC 2 cut(s) 922, 1905
XapI RAATTY 8 cut(s) 202, 293, 347, 651, 1127, 1142, 1570, 1612
XbaI TCTAGA 1 cut(s) 355
XceI RCATGY 3 cut(s) 119, 1807, 2105
XhoI CTCGAG 1 cut(s) 77
XmiI GTMKAC 2 cut(s) 410, 723
XmnI GAANNNNTTC 2 cut(s) 471, 807
XspI CTAG 4 cut(s) 168, 356, 719, 1863
ZrmI AGTACT 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.