RchiOBHm_Chr7g0223661

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000129
Physical Location & Seq
Forward (+)
45488771 .. 45496316
7546 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20025

Sequence Viewer

Length: 4905 bp
ATGATCACCCACACAGATATTCCTTCTTCTTCTTCCTCTCCTTCTCCGATCCCTCGATGGACGTACGACGTGTTCCTCAGTTTCAGGGGCGAAGATACCCGAAAAACTTTTACAGACCATCTCTACACCAGTCTTAAACAGAAAGGCATAATCACGTTCAGAGACGATGAGGGACTTGAGAGAGGAAAAGCCATTTCCCCAAAGCTGTTGAAAGCCATTGAAGAATCAAGATTCGTCATTGCCATCTTTTCAAGTAAGTACGCTGATTCAAGATGGTGCTTGGATGAGCTCGCAAAGGCTGTTGATTGTATGAACATGTTGGGACAAACGATACTCCCAGTCTTTTACCATGTGGATCCATCTGAGGTGAGAAAACAAACAGGAAAATTCGGCCAAGACTTGTGCATGCATCAACAACGCTTTAAAGACAAAATAGAGCAGGTGAATAGGTGGAGAGCTGCTCTTACGCAAGTCGCCAATCTCTCTGGATGGCATTTGCAGGATGGATCATATGAATCAAAAGTTATTCAAGAGATTGTAGGGCATATTTTCAACAAGTTGAACGAAACAATCTCAAGTGGATCCATGGATTTAGTTGGTATCGATTCTCGAATAAAACAAATCCTTTCATACTTAGACTTGGAGTTGAATGAAGTATGTATGATAGGTATTTCGGGAATGGGGGGAATCGGTAAGACAACTACTGCACAAGTAGTTTATGAAAGGATACGTGCTCAGTTTGAGGCTTACAGCTTTCTTGCAAATGTTAGAGAGACAACTGAGAAACAGGGCCTAGTTCACTTACAAAAACAACTTCTTTCAGACATTTTGCTGGAAAGTAATGTAAATATACAGAATATTGATATGGGAGTTAATATAATAAGCCAGAGACTACGAACTAAAATGGTTCTTATCATTCTTGATGATGTGAATGACTTGCAACAAATGAAAGCATTGTGTGGTCATAGTTGGTTTGGTTCTGGGAGCAGGATCATAATAACATCAAGGGATGAACAATTGCTTCGCACATATGGAGTGGAAAGAATATACAAGGTCAAGGCATTAAACGACCCGGAAGCTCTTCAGCTCTTTAGTTGGAAAGCATTCAGGAAAGATAAGGTTGGAGAAGATTTTCTCGTGCTATCAAAGAGTGTTTTAGAATATGCTAATGGGCTTCCATTAGCTATTGAAGTTTTGGGTTCCTTACTCTTTGGTAAAAGTGTAAAAGTATGGACAAGTGCATTGGATAGACTTAAAGTTAATCCTGACAAAAGAATAATTGATGTTCTTAAAGTAACTTTTGATGCATTAGAAGACACAGAAAAGGAAGTCTTTCTGGACATTGCATGTTTCTTTAAAGGGGAGGACAGAGATCGTGTAACAAGAATACTAGAAAGTAGTTGTGGCTACTCTCCAGACATTGATATAGAAGTTCTCATTGATAAGTCGATGGTAACTCTATTTGGAAGACAATTGTGGATGCATGACTTGATACAAGAATTGGGTTGGGAAATTGTTCGTCGGGAATGTCGTGATGAGCCGTGCAAACGTAGCAGGTTGTGGCTTCCAAAAGATATCATTCCTTTACTTGAACATAATCAGGGAACGAGTGCAGTGGAATCTATTTTGCTGAATTTGCCAAAACAAAAGGAGGTTCACTTGAATGTTGATCCCTTCTCAAAGATGTGCAAGTTAAGATTGCTCAAAATTAATAATGCACGCTTTTCTGGAGACATCAAATGGCTTTCTAATGAGTTACAAGTTCTTGAATGGCATGTTTGTCCTTTATATTCTTTGCCATCAAACTTTCAATCAGATAAGCTTGTTGAACTTAGCATGCATTCAAGCTGTATCAAACAGCTATGGAAGGGAAATAAGCGGTTGAGCATGCTAAAGTTTATCGATATGAGTGATTCTCAATACTTGATCAGGACCCCAAGCTTTGTTGAGGTTCCAAACATTGAGACTTTGGTGCTTGAAGGCTGTACAAGATTGAGCCTTCCACCTAACATTAGTTTGGAATCCCTTACAATCTTTAATCTCTCTGGCTGCTCAAGCTTGAAGAAGTTTCCGGAAATTGAAGGAAACATGCAGAGCTTGTTGGAGCTTCATCTAGATGGGACTGCCATAGAGGAATTACCTGCATCAATTGAAAGTTTAACTGGTCTCACTTCGTTGAATTTAGGAGACTGCACAAATCTGTTCCGGCTTCCAAGTACCATTCAATTCTTGACATCTCTGAAAAGTCTCATTCTTACAGGTTGCTCAGAACTTGGAAACATACCAGAGAATCTGAATTGTGTGGAATGTTTGGAGGATCTTGATATAAGTGGAACTGCTATAAGAGAGTTATCGTGCATTGCAGATATGAAAAATCTCAAGTCTCTTTGTTTCAGAGGAGGTAAAAATCTACCTCCGAAATCCTGGCATTCACTGTTCAATTGTTGGGGGATGTTTGGAAGGAAAGATGTTCCTGTGGGCTTGTTGCTGCCCGCTTCCTTATCAGGTTTGAGTTCTTTGACATATCTAAACTTCAGTGAGTGCAATCTGATGGATGGAGATATTCCAAATGATCTTGGGAGTTTAGTCTCTCTAAGAAAATTGATTTTAAGTGGTAATAAGTTTTCTTGCTTACCGGAAAGCATCTCTCAGCTCTCTAAGCTTCAAACTCTCGACTTGAACAATTGTAGCCGGCTTCAATCGCTGCCTAAGAAACTCCCATTAAGTGTTCGAGCTGTGAATGCAGATGACTGTACATCACTGATGGATTATCCAGATCAATTCTTAGTATGGACTTCATCTGTGTCAGGAATGACAACTATGAGTTCATTGATTTCATCCAAGCATAAAAAATATTCCACTTCCTCACCAAGACAAATCTCATATCATTCCAGAACATGGATTCAGTCAACTGTCCTTGGACGTGGTTTCTTTAGTTTTAGCCATCAACAAGAATGTAAACCAGTTGATGATTTATCAATGCCAAATCAAGTGCTTCAGAAAGACCTTGAAGCATTGGATTACAAGTCTTGTTCCCTGATTTCCGGCTGTGAAGAAAATGAAATTCCAGAGTGGTTTAGCAATATAGATACCGGCAGCTCCGTAGGAATCTCGATACCTGCAGGACTCAAAGATGATGAGCAATGGATGGGGGTTTCTGCTGTTTTCTTAATCAAGGGACATCCTCATTTCTCCGACATTGAATCAGAATCAGAAACACCTACTTACTTCTATAAATGCACGGTAGTAACTCAAGAATGTCAAGTGGAACCTGTTCTCCTTGAATGGAACCATCTCAACTGCATTCAATATAGTTCTCAGTTTCTTTGCTTTTTCTATGTACCTTGCCAATTCTTTCCCAAAAGGTTAAATGAATCAAGTGTAATGTGGGCTGTCTTTGAGACCAATAACCCATGCATGGAGGTCCAGAAATGTGGGATTCGTCTACTGTTCAAGCAAGGTGTTGCAGGGTTTATCCAGACATTCATGCCTCCCACTCTTGGTATTGGAGGTCGTACGCATGATCTTGACCCTCTGCTTGCAATAAATGCCAGCCGAATCGTCCCCCCTGACGACTGCATCAATCTGATTGAAGTTGACAAAGCTACAATTGATTCAGGATGGTTTAATCTAGTAGTGGATCGCACTTTGAGATGGGAGAAATTACTTCCAACTTATGAAGAAGGCTCCAAACTGCCTAGAATTAGGAACATGGAGTCATTTCTATCAAGATTCCTTGAGGGACTAAACCATAAGGCTCAACCCTATGATTTTCTTGTAAGTGGAAGCCCAGCGTGGTTCAAGACTGAAGTTGGGTCCACGGTGTCAATCCAGTTGCCCCCTCATGAGAATCTACACAAGAGTAAAAAGTGGATGGGATTTGCTCTATGCACATCACTCGTTGTGGACAAGCATACAGCAAAGGAGGATTATGTTGGTTCTTGCCGACTCCAAAGGGGCAGATCTCATGCCATTGGATTTAATCTTACCCTGGACTTTAGACCCTTCATGATAGCTGATGCACATCAACTTTTGGTTTTTTATTTACCGCGGGCAGTGATTCCCCAACTGTTCATTGAAGCATCATCAAGAGAAATATTCACTTCCTATGAAACCGGTAGCCCTGGTGTTAAGGTTCAAATTTGTGGACTTCGTATTTTGTACAAGCAAGATTTGCAAGGGTTTGTCCAAACAATCACCCACTGCATATTACGCAGCTCAGGTGCCCCCTTTGAAGTTTACAATAAACTGGTGGTAGAAGATTGGTTAAGTTTGGTAAGGTTGCCGAGTCATAAATTCAATGCTTCCCGAGATTGTGATTCCAGTACCCGCCATAAAAGACAGTTTGAGTTGATCTCTCAAAGCTATAGAAATATGGACTGGTCTGTGGAAAACCTGTCATGCACGTTCCCTGGAATTGAAATTCCTGAGTGGTTCATAAATGTGGAAAAAAGCGACTCCGCTAAAATGCAACTAAATGGTAATTGGATGGGAATTGCAGTGTGTGCTTCTCTATCGATCCACGAGCATCCGAATATCATTTTTGACAATCCGTATGCAGAGTCTACTCGTCACGACATTTTTTGTTATCTAAAGGGCAATATTGCTTCTGCTGGTTCTCTCCGTTATAAGTTTGATACAGACAAAGAAATGTGGATGCATCCATGCAATTTCATTTGCTTTGTCTATATGCCACGGGCTTATTTTCCTGATTCATGGAATCAATGTGATACAATCGAGGGAAGTATTGGGTCTGACAGCTCAGGCTTTGGAATTCACAAGTGTGCCCTTGGTCTAGTATTTGAGGAGGATGTGGAGGATCTTGTACGAACGTTGACTTACTGCCACTTATCAGCGCACTGTCGTCAGTGTGAAGAAGCACAATATCGTAGTCAAAAGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

1634

Amino Acids

185.07

Weight (kDa)

5.94

Isoelectric Point (pI)

53.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 22 - 195 3.4e-59 TIR domain
TIR_2 PF13676 24 - 121 4.5e-16 TIR domain
NB-ARC PF00931 205 - 372 3.7e-19 NB-ARC domain
NACHT PF05729 224 - 364 5.8e-06 NACHT domain
WHD_ROQ1 PF23282 439 - 508 1.9e-17 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 659 - 798 1.9e-11 Leucine-rich repeat region
LRR_13 PF23286 673 - 759 1.8e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 832 - 905 1.3e-08 Leucine-rich repeat region
LRR_13 PF23286 839 - 923 3.3e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4629
AarI CACCTGC 1 cut(s) 430
Acc36I ACCTGC 4 cut(s) 430, 1545, 2149, 3127
AccB1I GGYRCC 1 cut(s) 4223
AccB7I CCANNNNNTGG 1 cut(s) 2898
AccI GTMKAC 2 cut(s) 3445, 4565
AccII CGCG 1 cut(s) 4051
AccIII TCCGGA 1 cut(s) 2071
AciI CCGC 6 cut(s) 1879, 2493, 4049, 4051, 4330, 4461
AclI AACGTT 1 cut(s) 4832
AcoI YGGCCR 1 cut(s) 391
AcsI RAATTY 8 cut(s) 386, 1633, 2179, 3063, 4140, 4295, 4422, 4773
AcuI CTGAAG 4 cut(s) 1067, 2518, 2981, 3828
AfiI CCNNNNNNNGG 3 cut(s) 2898, 3418, 3500
AflIII ACRYGT 2 cut(s) 69, 315
AgeI ACCGGT 1 cut(s) 4115
AjiI CACGTC 2 cut(s) 70, 2924
AjnI CCWGG 4 cut(s) 2423, 3990, 4123, 4411
AleI CACNNNNGTG 1 cut(s) 4782
AloI GAACNNNNNNTCC 2 cut(s) 3261, 3293
Alw21I GWGCWC 2 cut(s) 291, 736
Ama87I CYCGRG 1 cut(s) 4308
Aor13HI TCCGGA 1 cut(s) 2071
AoxI GGCC 2 cut(s) 391, 790
ApeKI GCWGC 6 cut(s) 458, 2049, 2488, 2704, 3096, 4215
ApoI RAATTY 8 cut(s) 386, 1633, 2179, 3063, 4140, 4295, 4422, 4773
AseI ATTAAT 1 cut(s) 1710
AsiGI ACCGGT 1 cut(s) 4115
Asp700I GAANNNNTTC 6 cut(s) 1080, 1103, 1131, 1332, 1515, 3273
AspLEI GCGC 1 cut(s) 4858
AspS9I GGNCC 4 cut(s) 790, 1932, 3424, 3816
AsuC2I CCSGG 1 cut(s) 1073
AsuHPI GGTGA 4 cut(s) 379, 454, 2859, 4189
AvaI CYCGRG 1 cut(s) 4308
AvaII GGWCC 3 cut(s) 1932, 3424, 3816
BaeGI GKGCMC 2 cut(s) 4228, 4789
BamHI GGATCC 2 cut(s) 355, 581
BanI GGYRCC 1 cut(s) 4223
BanII GRGCYC 1 cut(s) 291
BauI CACGAG 2 cut(s) 1136, 4523
BbsI GAAGAC 2 cut(s) 1320, 1474
Bbv12I GWGCWC 2 cut(s) 291, 736
BbvI GCAGC 6 cut(s) 445, 2036, 2475, 2691, 3108, 4227
BceAI ACGGC 1 cut(s) 1525
BcgI CGANNNNNNTGC 2 cut(s) 3880, 3914
BciT130I CCWGG 4 cut(s) 2425, 3992, 4125, 4413
BciVI GTATCC 1 cut(s) 720
BclI TGATCA 2 cut(s) 3, 1926
BcnI CCSGG 1 cut(s) 1073
BfaI CTAG 6 cut(s) 794, 1391, 2114, 3632, 3699, 4796
BfmI CTRYAG 2 cut(s) 3120, 4366
BfuAI ACCTGC 4 cut(s) 430, 1545, 2149, 3127
BfuI GTATCC 1 cut(s) 720
BglII AGATCT 1 cut(s) 3962
BisI GCNGC 6 cut(s) 459, 2050, 2489, 2705, 3097, 4216
BlsI GCNGC 6 cut(s) 460, 2051, 2490, 2706, 3098, 4217
Bme1390I CCNGG 5 cut(s) 1073, 2425, 3992, 4125, 4413
Bme18I GGWCC 3 cut(s) 1932, 3424, 3816
BmeT110I CYCGRG 1 cut(s) 4308
BmgBI CACGTC 2 cut(s) 70, 2924
BmgT120I GGNCC 4 cut(s) 790, 1932, 3424, 3816
BmrFI CCNGG 5 cut(s) 1073, 2425, 3992, 4125, 4413
BmrI ACTGGG 1 cut(s) 332
BmuI ACTGGG 1 cut(s) 332
BpiI GAAGAC 2 cut(s) 1320, 1474
BplI GAGNNNNNCTC 6 cut(s) 445, 477, 1900, 1932, 4340, 4372
BpmI CTGGAG 2 cut(s) 1398, 1749
Bpu10I CCTNAGC 2 cut(s) 4219, 4762
BpuEI CTTGAG 6 cut(s) 197, 559, 2038, 2363, 3237, 3758
BpuMI CCSGG 1 cut(s) 1073
Bsa29I ATCGAT 3 cut(s) 603, 1902, 4517
BsaAI YACGTR 1 cut(s) 731
BsaBI GATNNNNATC 1 cut(s) 4023
BsaI GGTCTC 2 cut(s) 2171, 3395
BsaJI CCNNGG 9 cut(s) 585, 2917, 3819, 3990, 4049, 4123, 4411, 4694, 4789
BsaWI WCCGGW 3 cut(s) 2071, 2635, 4115
BsaXI ACNNNNNCTCC 2 cut(s) 3261, 3291
Bsc4I CCNNNNNNNGG 3 cut(s) 2898, 3418, 3500
Bse118I RCCGGY 3 cut(s) 2691, 3092, 4115
Bse1I ACTGG 8 cut(s) 129, 338, 2167, 2963, 3832, 4254, 4323, 4385
Bse3DI GCAATG 4 cut(s) 237, 1341, 2358, 3149
Bse8I GATNNNNATC 1 cut(s) 4023
BseAI TCCGGA 1 cut(s) 2071
BseBI CCWGG 4 cut(s) 2425, 3992, 4125, 4413
BseCI ATCGAT 3 cut(s) 603, 1902, 4517
BseDI CCNNGG 9 cut(s) 585, 2917, 3819, 3990, 4049, 4123, 4411, 4694, 4789
BseJI GATNNNNATC 1 cut(s) 4023
BseLI CCNNNNNNNGG 3 cut(s) 2898, 3418, 3500
BseMI GCAATG 4 cut(s) 237, 1341, 2358, 3149
BseNI ACTGG 8 cut(s) 129, 338, 2167, 2963, 3832, 4254, 4323, 4385
BseRI GAGGAG 2 cut(s) 2412, 4820
BseSI GKGCMC 2 cut(s) 4228, 4789
BseXI GCAGC 6 cut(s) 445, 2036, 2475, 2691, 3108, 4227
BseYI CCCAGC 1 cut(s) 3790
BsgI GTGCAG 3 cut(s) 690, 1632, 2176
Bsh1236I CGCG 1 cut(s) 4051
BshFI GGCC 2 cut(s) 393, 792
BshNI GGYRCC 1 cut(s) 4223
BshTI ACCGGT 1 cut(s) 4115
BshVI ATCGAT 3 cut(s) 603, 1902, 4517
BsiHKAI GWGCWC 2 cut(s) 291, 736
BsiHKCI CYCGRG 1 cut(s) 4308
BsiSI CCGG 8 cut(s) 1073, 2072, 2206, 2636, 2692, 3045, 3093, 4116
BsiWI CGTACG 2 cut(s) 63, 3515
BslFI GGGAC 6 cut(s) 186, 336, 2134, 3192, 3548, 3756
BslI CCNNNNNNNGG 3 cut(s) 2898, 3418, 3500
BsmBI CGTCTC 1 cut(s) 156
BsmFI GGGAC 6 cut(s) 186, 336, 2134, 3192, 3548, 3756
BsmI GAATGC 5 cut(s) 1103, 1840, 2428, 2746, 3303
BsnI GGCC 2 cut(s) 393, 792
Bso31I GGTCTC 2 cut(s) 2171, 3395
BsoBI CYCGRG 1 cut(s) 4308
Bsp1286I GDGCHC 4 cut(s) 291, 736, 4228, 4789
Bsp13I TCCGGA 1 cut(s) 2071
Bsp1407I TGTACA 3 cut(s) 1985, 2753, 4161
Bsp19I CCATGG 1 cut(s) 585
BspACI CCGC 6 cut(s) 1879, 2493, 4049, 4051, 4330, 4461
BspANI GGCC 2 cut(s) 393, 792
BspDI ATCGAT 3 cut(s) 603, 1902, 4517
BspEI TCCGGA 1 cut(s) 2071
BspFNI CGCG 1 cut(s) 4051
BspHI TCATGA 2 cut(s) 3844, 4008
BspMAI CTGCAG 1 cut(s) 3124
BspMI ACCTGC 4 cut(s) 430, 1545, 2149, 3127
BspQI GCTCTTC 1 cut(s) 1086
BspT107I GGYRCC 1 cut(s) 4223
BspTNI GGTCTC 2 cut(s) 2171, 3395
BsrDI GCAATG 4 cut(s) 237, 1341, 2358, 3149
BsrFI RCCGGY 3 cut(s) 2691, 3092, 4115
BsrGI TGTACA 3 cut(s) 1985, 2753, 4161
BsrI ACTGG 8 cut(s) 129, 338, 2167, 2963, 3832, 4254, 4323, 4385
BssAI RCCGGY 3 cut(s) 2691, 3092, 4115
BssECI CCNNGG 9 cut(s) 585, 2917, 3819, 3990, 4049, 4123, 4411, 4694, 4789
BssSI CACGAG 2 cut(s) 1136, 4523
BssT1I CCWWGG 3 cut(s) 585, 2917, 4789
Bst2BI CACGAG 2 cut(s) 1136, 4523
Bst2UI CCWGG 4 cut(s) 2425, 3992, 4125, 4413
Bst4CI ACNGT 9 cut(s) 2436, 2753, 2914, 3244, 3450, 3823, 4071, 4344, 4862
Bst6I CTCTTC 1 cut(s) 1086
BstAPI GCANNNNNTGC 3 cut(s) 3548, 4174, 4505
BstAUI TGTACA 3 cut(s) 1985, 2753, 4161
BstBAI YACGTR 1 cut(s) 731
BstDSI CCRYGG 4 cut(s) 585, 3819, 4049, 4694
BstFNI CGCG 1 cut(s) 4051
BstHHI GCGC 1 cut(s) 4858
BstNI CCWGG 4 cut(s) 2425, 3992, 4125, 4413
BstNSI RCATGY 7 cut(s) 319, 409, 1350, 1778, 1840, 1891, 2092
BstSCI CCNGG 5 cut(s) 1071, 2423, 3990, 4123, 4411
BstSFI CTRYAG 2 cut(s) 3120, 4366
BstSLI GKGCMC 2 cut(s) 4228, 4789
BstUI CGCG 1 cut(s) 4051
BstV1I GCAGC 6 cut(s) 445, 2036, 2475, 2691, 3108, 4227
BstV2I GAAGAC 2 cut(s) 1320, 1474
BstX2I RGATCY 5 cut(s) 355, 581, 2317, 3962, 4819
BstXI CCANNNNNNTGG 1 cut(s) 3434
BstYI RGATCY 5 cut(s) 355, 581, 2317, 3962, 4819
Bsu15I ATCGAT 3 cut(s) 603, 1902, 4517
BsuI GTATCC 1 cut(s) 720
BsuRI GGCC 2 cut(s) 393, 792
BsuTUI ATCGAT 3 cut(s) 603, 1902, 4517
BtgI CCRYGG 4 cut(s) 585, 3819, 4049, 4694
BtrI CACGTC 2 cut(s) 70, 2924
BtsI GCAGTG 4 cut(s) 1620, 4062, 4201, 4506
BtsIMutI CAGTG 9 cut(s) 1620, 2432, 2542, 2759, 4062, 4201, 4506, 4858, 4874
BveI ACCTGC 4 cut(s) 430, 1545, 2149, 3127
CciI TCATGA 2 cut(s) 3844, 4008
CfoI GCGC 1 cut(s) 4858
Cfr10I RCCGGY 3 cut(s) 2691, 3092, 4115
Cfr13I GGNCC 4 cut(s) 790, 1932, 3424, 3816
Cfr42I CCGCGG 1 cut(s) 4052
ClaI ATCGAT 3 cut(s) 603, 1902, 4517
CspAI ACCGGT 1 cut(s) 4115
DraI TTTAAA 2 cut(s) 424, 1357
EaeI YGGCCR 1 cut(s) 391
Eam1104I CTCTTC 1 cut(s) 1086
EarI CTCTTC 1 cut(s) 1086
Ecl136II GAGCTC 1 cut(s) 289
Eco130I CCWWGG 3 cut(s) 585, 2917, 4789
Eco24I GRGCYC 1 cut(s) 291
Eco31I GGTCTC 2 cut(s) 2171, 3395
Eco32I GATATC 1 cut(s) 1576
Eco47I GGWCC 3 cut(s) 1932, 3424, 3816
Eco53kI GAGCTC 1 cut(s) 289
Eco57I CTGAAG 4 cut(s) 1067, 2518, 2981, 3828
Eco88I CYCGRG 1 cut(s) 4308
EcoICRI GAGCTC 1 cut(s) 289
EcoO109I RGGNCCY 2 cut(s) 790, 1932
EcoRI GAATTC 1 cut(s) 4773
EcoRII CCWGG 4 cut(s) 2423, 3990, 4123, 4411
EcoRV GATATC 1 cut(s) 1576
EcoT14I CCWWGG 3 cut(s) 585, 2917, 4789
EcoT22I ATGCAT 6 cut(s) 411, 1309, 1485, 1842, 3419, 4662
EcoT38I GRGCYC 1 cut(s) 291
ErhI CCWWGG 3 cut(s) 585, 2917, 4789
Esp3I CGTCTC 1 cut(s) 156
FalI AAGNNNNNCTT 2 cut(s) 1316, 1348
FaqI GGGAC 6 cut(s) 186, 336, 2134, 3192, 3548, 3756
FauI CCCGC 3 cut(s) 2500, 4044, 4337
FauNDI CATATG 2 cut(s) 511, 1030
FbaI TGATCA 2 cut(s) 3, 1926
FblI GTMKAC 2 cut(s) 3445, 4565
Fnu4HI GCNGC 6 cut(s) 459, 2050, 2489, 2705, 3097, 4216
FriOI GRGCYC 1 cut(s) 291
Fsp4HI GCNGC 6 cut(s) 459, 2050, 2489, 2705, 3097, 4216
FspBI CTAG 6 cut(s) 794, 1391, 2114, 3632, 3699, 4796
GlaI GCGC 1 cut(s) 4857
GluI GCNGC 6 cut(s) 459, 2050, 2489, 2705, 3097, 4216
GsaI CCCAGC 1 cut(s) 3794
GsuI CTGGAG 2 cut(s) 1398, 1749
HaeIII GGCC 2 cut(s) 393, 792
HapII CCGG 8 cut(s) 1073, 2072, 2206, 2636, 2692, 3045, 3093, 4116
HhaI GCGC 1 cut(s) 4858
Hin6I GCGC 1 cut(s) 4856
HinP1I GCGC 1 cut(s) 4856
HincII GTYRAC 3 cut(s) 2910, 3598, 4836
HindII GTYRAC 3 cut(s) 2910, 3598, 4836
HindIII AAGCTT 4 cut(s) 1820, 1939, 2056, 2660
HpaII CCGG 8 cut(s) 1073, 2072, 2206, 2636, 2692, 3045, 3093, 4116
HphI GGTGA 4 cut(s) 379, 454, 2859, 4189
Hpy99I CGWCG 2 cut(s) 71, 1524
HpyCH4III ACNGT 9 cut(s) 2436, 2753, 2914, 3244, 3450, 3823, 4071, 4344, 4862
HpyCH4IV ACGT 8 cut(s) 62, 69, 155, 730, 1549, 2923, 4406, 4832
HpySE526I ACGT 8 cut(s) 62, 69, 155, 730, 1549, 2923, 4406, 4832
HspAI GCGC 1 cut(s) 4856
Kpn2I TCCGGA 1 cut(s) 2071
KroI GCCGGC 1 cut(s) 2691
KroNI GCCGGC 1 cut(s) 2693
Ksp22I TGATCA 2 cut(s) 3, 1926
KspI CCGCGG 1 cut(s) 4052
LguI GCTCTTC 1 cut(s) 1086
LmnI GCTCC 4 cut(s) 984, 2104, 3104, 3692
Lsp1109I GCAGC 6 cut(s) 445, 2036, 2475, 2691, 3108, 4227
MaeI CTAG 6 cut(s) 794, 1391, 2114, 3632, 3699, 4796
MaeII ACGT 8 cut(s) 62, 69, 155, 730, 1549, 2923, 4406, 4832
MaeIII GTNAC 6 cut(s) 1294, 1378, 1453, 1755, 3247, 4571
MfeI CAATTG 6 cut(s) 1016, 1472, 2148, 2440, 2683, 3609
MflI RGATCY 5 cut(s) 355, 581, 2317, 3962, 4819
MhlI GDGCHC 4 cut(s) 291, 736, 4228, 4789
MlyI GAGTC 6 cut(s) 3120, 3725, 3942, 4297, 4451, 4571
MmeI TCCRAC 5 cut(s) 1076, 1102, 2082, 3219, 3695
Mph1103I ATGCAT 6 cut(s) 411, 1309, 1485, 1842, 3419, 4662
MroI TCCGGA 1 cut(s) 2071
MroNI GCCGGC 1 cut(s) 2691
MroXI GAANNNNTTC 6 cut(s) 1080, 1103, 1131, 1332, 1515, 3273
MslI CAYNNNNRTG 4 cut(s) 1662, 2115, 4442, 4782
MspA1I CMGCKG 1 cut(s) 4051
MspI CCGG 8 cut(s) 1073, 2072, 2206, 2636, 2692, 3045, 3093, 4116
MspR9I CCNGG 5 cut(s) 1073, 2425, 3992, 4125, 4413
MunI CAATTG 6 cut(s) 1016, 1472, 2148, 2440, 2683, 3609
Mva1269I GAATGC 5 cut(s) 1103, 1840, 2428, 2746, 3303
MvaI CCWGG 4 cut(s) 2425, 3992, 4125, 4413
MvnI CGCG 1 cut(s) 4051
NaeI GCCGGC 1 cut(s) 2693
NciI CCSGG 1 cut(s) 1073
NcoI CCATGG 1 cut(s) 585
NdeI CATATG 2 cut(s) 511, 1030
NgoMIV GCCGGC 1 cut(s) 2691
NmeAIII GCCGAG 1 cut(s) 4311
NmuCI GTSAC 1 cut(s) 4571
NsiI ATGCAT 6 cut(s) 411, 1309, 1485, 1842, 3419, 4662
NspI RCATGY 7 cut(s) 319, 409, 1350, 1778, 1840, 1891, 2092
OliI CACNNNNGTG 1 cut(s) 4782
PaeI GCATGC 3 cut(s) 409, 1840, 1891
PagI TCATGA 2 cut(s) 3844, 4008
PaqCI CACCTGC 1 cut(s) 430
PciI ACATGT 1 cut(s) 315
PciSI GCTCTTC 1 cut(s) 1086
PcsI WCGNNNNNNNCGW 1 cut(s) 1528
PctI GAATGC 5 cut(s) 1103, 1840, 2428, 2746, 3303
PdiI GCCGGC 1 cut(s) 2693
PdmI GAANNNNTTC 6 cut(s) 1080, 1103, 1131, 1332, 1515, 3273
Pfl23II CGTACG 2 cut(s) 63, 3515
PflMI CCANNNNNTGG 1 cut(s) 2898
PinAI ACCGGT 1 cut(s) 4115
PkrI GCNGC 6 cut(s) 460, 2051, 2490, 2706, 3098, 4217
PleI GAGTC 6 cut(s) 3120, 3724, 3942, 4296, 4451, 4570
PpsI GAGTC 6 cut(s) 3120, 3724, 3942, 4296, 4451, 4570
Ppu21I YACGTR 1 cut(s) 731
PpuMI RGGWCCY 1 cut(s) 1932
PscI ACATGT 1 cut(s) 315
PshBI ATTAAT 1 cut(s) 1710
PsiI TTATAA 1 cut(s) 4629
Psp124BI GAGCTC 1 cut(s) 291
Psp1406I AACGTT 1 cut(s) 4832
Psp5II RGGWCCY 1 cut(s) 1932
Psp6I CCWGG 4 cut(s) 2423, 3990, 4123, 4411
PspFI CCCAGC 1 cut(s) 3790
PspGI CCWGG 4 cut(s) 2423, 3990, 4123, 4411
PspLI CGTACG 2 cut(s) 63, 3515
PspPI GGNCC 4 cut(s) 790, 1932, 3424, 3816
PspPPI RGGWCCY 1 cut(s) 1932
PsrI GAACNNNNNNTAC 2 cut(s) 56, 88
PstI CTGCAG 1 cut(s) 3124
PsuI RGATCY 5 cut(s) 355, 581, 2317, 3962, 4819
RseI CAYNNNNRTG 4 cut(s) 1662, 2115, 4442, 4782
SacI GAGCTC 1 cut(s) 291
SacII CCGCGG 1 cut(s) 4052
SapI GCTCTTC 1 cut(s) 1086
SatI GCNGC 6 cut(s) 459, 2050, 2489, 2705, 3097, 4216
Sau96I GGNCC 4 cut(s) 790, 1932, 3424, 3816
SbfI CCTGCAGG 1 cut(s) 3124
SchI GAGTC 6 cut(s) 3120, 3725, 3942, 4297, 4451, 4571
ScrFI CCNGG 5 cut(s) 1073, 2425, 3992, 4125, 4413
SdaI CCTGCAGG 1 cut(s) 3124
SduI GDGCHC 4 cut(s) 291, 736, 4228, 4789
SfcI CTRYAG 2 cut(s) 3120, 4366
Sfr303I CCGCGG 1 cut(s) 4052
SgrBI CCGCGG 1 cut(s) 4052
SinI GGWCC 3 cut(s) 1932, 3424, 3816
SmiMI CAYNNNNRTG 4 cut(s) 1662, 2115, 4442, 4782
SmlI CTYRAG 6 cut(s) 176, 574, 2053, 2378, 3252, 3737
SmoI CTYRAG 6 cut(s) 176, 574, 2053, 2378, 3252, 3737
SphI GCATGC 3 cut(s) 409, 1840, 1891
Sse8387I CCTGCAGG 1 cut(s) 3124
SsiI CCGC 6 cut(s) 1879, 2493, 4049, 4051, 4330, 4461
SspI AATATT 4 cut(s) 859, 2855, 4098, 4603
SspMI CTAG 6 cut(s) 794, 1391, 2114, 3632, 3699, 4796
SstI GAGCTC 1 cut(s) 291
StyD4I CCNGG 5 cut(s) 1071, 2423, 3990, 4123, 4411
StyI CCWWGG 3 cut(s) 585, 2917, 4789
TaaI ACNGT 9 cut(s) 2436, 2753, 2914, 3244, 3450, 3823, 4071, 4344, 4862
TaiI ACGT 8 cut(s) 65, 72, 158, 733, 1552, 2926, 4409, 4835
TatI WGTACW 3 cut(s) 1985, 2753, 4161
TscAI CASTG 9 cut(s) 1620, 2439, 2542, 2766, 4062, 4208, 4506, 4865, 4874
TseFI GTSAC 1 cut(s) 4571
TseI GCWGC 6 cut(s) 458, 2049, 2488, 2704, 3096, 4215
Tsp45I GTSAC 1 cut(s) 4571
TspGWI ACGGA 3 cut(s) 3091, 4542, 4613
TspRI CASTG 9 cut(s) 1620, 2439, 2542, 2766, 4062, 4208, 4506, 4865, 4874
Van91I CCANNNNNTGG 1 cut(s) 2898
VpaK11BI GGWCC 3 cut(s) 1932, 3424, 3816
VspI ATTAAT 1 cut(s) 1710
XapI RAATTY 8 cut(s) 386, 1633, 2179, 3063, 4140, 4295, 4422, 4773
XbaI TCTAGA 1 cut(s) 2113
XceI RCATGY 7 cut(s) 319, 409, 1350, 1778, 1840, 1891, 2092
XmiI GTMKAC 2 cut(s) 3445, 4565
XmnI GAANNNNTTC 6 cut(s) 1080, 1103, 1131, 1332, 1515, 3273
XspI CTAG 6 cut(s) 794, 1391, 2114, 3632, 3699, 4796
Zsp2I ATGCAT 6 cut(s) 411, 1309, 1485, 1842, 3419, 4662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.