Rw7G041440

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000129
Physical Location & Seq
Forward (+)
64659011 .. 64681748
22738 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G041440.1

Sequence Viewer

Length: 4521 bp
ATGTTAAGCCGTTTGAGGTACGAAGACAAAAAGGTGATCACTTTGAGAAAGCATTTCAGAAGCATCGAGAGCGGTGTAAAGAAGAACCAGGCAAAGTGGAAAGACGCTCTTGCTGAAGTAGGCAATCTTTCTGGATGGCATTTGCAAAATGGGTCTGAAGCCAAAATTATTCAAGAAATTGTAGAAAAGGTTTTCACTGAGTTGAATAAAATAATATCCACATCTGAGGGCTTAGTTGGGATGGATTCTCACCTGAATGAACTGTTTTCCTACTTAGACATTGGGTGTCTCAAAGATGTTCGCATCATAGGGATTTGTGGGATGGGTGGTATCGGGAAAACTACTATTGCACAAGTGGTTTTTGAAAGGCTAAGAGCTCAGTTTGAAGGTGATAGCTTTCTTGAGAATGTTAAACAGGAAACTGAAATGAAGCAAGGTAGTACAATTCATTTACAAGAGAAACTTCTCTTGAACTTGCTGAATAGTAATGTCAATGTACAGAACACTAAAATGGGAAAAGACATAATTAAGCATAGACTATCTACTAAAAGGGTGCTTATTGTTCTTGATGATGTGGATCAAGATAAACAATTGGAAACATTGTGTGGTCGTACTTGGTTTGGTCCAGGGAGTAGAATCATCATAACTTCCAGAGATGAACACCTGATTAGTGCATTTGGAGCAGATAAAGTATACAAGGTGAATGCATTAACTGATCCTGAAGCTTTTGAGCTCTTTCATTTGAAAGCCTTCAATGAAGACGAGGTTGGAGAAGATTTTCTCAAGCTATCCAAGGAATTTTTGAAATATGCTAATGGCCTTCCATTAGCTATTAAAGTTTTGGGATCTTCTGTTTGTGGTAGAAAAGTAAAAATATGGTCAAGTGCATTGAAGAGACTTAAAAAAAATCCTCAGAAAGGGATTATTGATGTTCTTAAAGTTAGTTTTGATGGATTAGAAGAATCAGAAAAGAAAACCTTTTTGGACATCGCGTGTTTCTTTAAAGGAGAGAACAAGGATCGTGTAATAAGAATACTAAATGGTTGTGACGATGACGGTGTGGACATGGATGTGCAAGTTCTTTTGGAAAAATCTTTGGTAACTCTATTTGGAACAAAATTGTGGATGCATGATTTGATACAAGAAATGGGTTGGGAAGTTGTTCTTCAAAAAAATCGTGAAGAGCCTGGAAAACGTAGCAGATTGTGGCTTCCCGATGAAATCATCCAATTACTTGGTCGGAGTAAGGTGACGAGTGAAGTTCAAAGCATATTCCTCCAATGTCCAACAGAAGACGATGTTGTTCACTCAATTGATAATGCTTTCTCAATTATGGACAGATTGAGATTGCTCAAGATTTGTAATGTGAAGTTTTCCGGAAACATCACATATCTTTCTAATGAGTTACAGTATCTTGAATGGCATGAGTGTCCTCTAGATTCTTTCCCGTCAGACTTTCAACCAAATAAACTTGTTGAAGTTCGCATGCATTTTAGCCGCATCAAACAATTATGGAGGGAAAAAAAAGGATGGAGCATGCTACGGCATATAGATCTGAGTGGTTCACTATACTTGATGTCCACTCCAGACTTCACTGAGGTTCCAGATCTTGAAAAGTTGGTGCTTCAATTTTGTATAAGCTTGGTGGAGGTTCACCCGTCTCTTGGATCTCTGAAGAATCTTGTCTCTTTGGATATGACATATTGCCAGTCTATTGAGAGCATTCCACCTTTCACTGCCCTTGAATCCCTTCAAATATTGAGTCTTTCATCCTGTTCAGGACTGAAGAAGTTTCCAGAAATCGAAGGAAATAATCGAAATATGAAAAGCTTGCTGGAGCTTCATTTGGATGGGACCAGCATTGAGGAATTGCCTCCATCAATTGAACGCTTGACTGGTCTAACCATGTTGGATCTGACAGATTGTAAAAACCTTTTGCATCTTCCCAACACCATTGGGTGCTTGACATCTTTGAAAAGTCTCTATCTAACAGATTGCTCCAAACTTGATGAGATACCTGAGAATCTGAATGGTATGAAATGTCTGGAGAAGCTTTTAATTGGTGGAACTTCCATAAGGGAGTTATCTTTCATTTTAGGCATGAAGAATCTACAGTATCTGTCCTGCCGAGGATGTAAATGTCTAGTTTCAGAATCATGCAAAGGTCTGTCTTCGTTATCAAGTTTGATGGAGCTAGACTTGAGTTATTGCAATCTGATGGATGGAGCAATTCTCAATGATTTTAGCAGCCTAATCTCCTTAAGAAGGTTAGATTTAAGTGGTAATTTCTTTGTGCGATTACCTGAAAGTATCACTCATTTCTCAAAGCTCGAGGCTCTGCAGTTGAGGAATTGTAGACAGCTCCAATTGCTGCCCAAGAAGCTTCCATCAAGTCTTCTAAACGTGTATGCAGAAGGATGTACTTCACTGACGGATTACCCAAATCAAATGAAAGTATTGACTTCACCGGAGTCGGGAGTGACTATTGTCGATTCCATCAATTCTTCAGCACTAGGACTCTCACAAAGTTTTGCAATGTTGGATTTTCCAAGAAATGAGGGGGAATCTCTAGAGCTGCGTGTGACATATGACCATACAGAGGGGCAACAAGTTGATCATTTGCCACTAGCAATTCCCGCGTTTACAAAACAACTGCTTTCGGGGTCAAAATTCAGTACCATACTTCCTCTAGATGAAATTCCAGAGTGGTTCATCACTACATCTACCAGAAATCCCATATCAATCTCGATACCTCCAAATCTGGCCGATGATAACACGTGGAATGGATTTGTCGCGTGTGCTATATTCTCAGTCAAGGGGCATACTGCTCTCTCCCTTATTGAACCGGATCCTGATTTTTCTAATTACTCATATCAAATCACATTGGAAACTGATGTTGTGCGTCTGGAGCCATATGTGTTGGGACTGGATTCAGTGGTATTAGGCCGCTTTAGATCAATTTCTCATCTGCTTGCGATTTTCTTTATATCTAGTTTGAAGTTTCCACAGAGGGGATTAAATCAATCAACTGTGGTAAGTGCTATGTTTGAAACCACCAATCCATCCATGGTGGTCCAGAAATGTGGAATTCGTCCAGTCTATGAGCAAGATGCTGGATGGTTCTCCAGAATAATCGGGCGGAATGATTTGATTTGTCTGAATAAAGTTGATGACACCACCTCTATATGGCTTGAACAATACACCGCCTGTGTATTGGAGTCTGGCTGGCTTACTCAATCTTTGGAATGGGAGGAATTCATTCCACCCTTGGAAGAAGAATCCACACTTGTGCTGAGAAAGAACCTTGAGTATGTTCTTCCGAGATACCTTGAGGCACTAAATCATTACTGTGCAACTTTTGAATTAAACATCAGAGGAAGCCCAGAGTGGTTTCAGTCGTTTGTCCCTAATACGAGGTTAAATTTCACCATAGGGTCAATCAAGCTGCCTCAAAATCTACATAAGAGCAAGAAATGGAAGGGATTAGCAATATACGCATCACTTGTAGAGGAAGTGGGACAGACAATGAAAGAGGACAACTATCGGATATATGTTACACTAACAACTGGGGATATATCTGGCAAGATGATGTCCCTAGAACTTAAGCATGTCAGGCCATTGTCAGAAGAACATCATCAACTATTGGTTATTTACATACCGCGAGCACAGATTCCCGAAGCGTTGTTCACTCAGACATTAACAACTGCAATGGACTTTTGCATACATATCAGTGATAGTCCTCATGTAAAGGTTCAAAGATGTGGGTTTCGTATTGTGTACCAGGAAGATATACAAGGGTTTGCTGATACAATTATCCGGTGCATGCAGAGGGAAGATTCTCTTGAATATTACAATAAGTTGGTGGTAGAACAATGGATAAAGTTGATACGATTGCAAGGTGCACATATAGAAGGAATGAAGGAATGGGATTTCAGAACCCCACGGGAAAAATGCTTTGAATTGCAGTCACGAAAATATAGAAATTGGGAATGGTCTGGCCCTCATCTTGCTTATTGTGTCTTTGACATGGTTGAAATCTCCAAGTTGAAATGGTTCATGCCTTACATCAACCAAGGCAACTTTGCAGAAATCCAACTACCTCTGAATGTGTTCGATGATGGTAATTGGTTGGGGTTTGCCGCGTGTTTTCAGTTGTCCGACAACCAATATCCAAACGTTAGTCTTGGCAGCACTGCTCCTGATCCAGAGGATGTTAATGCTATTTGCCGTCTGGACTCTGATGTTGCTTGGCAGTACTTATTTGATGTCGAGATGCATTTAAGTTTAAAGAGTCAAGAGCTATTGAAATGTGATACGACTTGGTTCTTTTATATACCGCGAACTGGAGTTCGTAGTAAGGTTTGGAGGCAATGCAAATTGGCAAGGGTAACTTTGTGGTTCTCTAGGCCATGGTTTGCAGTGCATAGTTGTGCCCTTCGTCTACTATTTAAGGAGGATGTTGAACATCTTGTAGAAACGCTGACCCATGCCGAATTACGTACCGTGAAGATCCTCCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1506

Amino Acids

171.77

Weight (kDa)

5.8

Isoelectric Point (pI)

41.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 98 - 254 2e-23 NB-ARC domain
WHD_ROQ1 PF23282 320 - 389 1.9e-12 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 568 - 707 4.8e-12 Leucine-rich repeat region
LRR_13 PF23286 581 - 671 2.5e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 722 - 821 1.7e-06 Leucine-rich repeat region
C-JID PF20160 895 - 1038 1e-07 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 72
AccI GTMKAC 3 cut(s) 693, 2356, 4441
AccII CGCG 6 cut(s) 992, 2639, 2795, 3661, 4142, 4339
AccIII TCCGGA 1 cut(s) 1376
AciI CCGC 9 cut(s) 72, 1498, 2637, 2947, 3139, 3204, 3659, 4140, 4337
AclI AACGTT 1 cut(s) 4176
AcoI YGGCCR 1 cut(s) 2763
AcsI RAATTY 6 cut(s) 797, 2669, 2697, 3087, 3254, 3421
AcuI CTGAAG 6 cut(s) 135, 177, 741, 1694, 1805, 2490
AcvI CACGTG 1 cut(s) 2778
AfaI GTAC 9 cut(s) 20, 442, 498, 613, 2422, 2677, 3779, 4256, 4501
AfiI CCNNNNNNNGG 8 cut(s) 917, 1664, 2265, 2474, 2599, 3011, 3186, 4343
AflII CTTAAG 2 cut(s) 2260, 3602
AflIII ACRYGT 2 cut(s) 2403, 2775
AjnI CCWGG 4 cut(s) 87, 625, 1186, 3780
AjuI GAANNNNNNNTTGG 4 cut(s) 750, 782, 965, 997
AleI CACNNNNGTG 1 cut(s) 3287
Alw21I GWGCWC 4 cut(s) 379, 735, 3667, 3904
Alw26I GTCTC 5 cut(s) 293, 889, 1665, 1690, 1985
Alw44I GTGCAC 1 cut(s) 3900
AlwNI CAGNNNCTG 1 cut(s) 2119
Ama87I CYCGRG 1 cut(s) 2330
Aor13HI TCCGGA 1 cut(s) 1376
AoxI GGCC 6 cut(s) 817, 2763, 2944, 3614, 3997, 4406
ApaLI GTGCAC 1 cut(s) 3900
ApeKI GCWGC 5 cut(s) 2247, 2371, 2575, 3445, 4188
ApoI RAATTY 6 cut(s) 797, 2669, 2697, 3087, 3254, 3421
ArsI GACNNNNNNTTYG 2 cut(s) 1747, 1779
Asp700I GAANNNNTTC 7 cut(s) 749, 777, 1161, 1749, 3258, 4052, 4109
AspS9I GGNCC 4 cut(s) 623, 1854, 3073, 3998
AsuHPI GGTGA 8 cut(s) 46, 242, 401, 712, 1261, 1646, 2457, 3418
AvaI CYCGRG 1 cut(s) 2330
AvaII GGWCC 3 cut(s) 623, 1854, 3073
BaeGI GKGCMC 2 cut(s) 3904, 4435
BamHI GGATCC 1 cut(s) 2848
BanII GRGCYC 2 cut(s) 379, 735
BbrPI CACGTG 1 cut(s) 2778
BbsI GAAGAC 5 cut(s) 30, 765, 1299, 2163, 2387
Bbv12I GWGCWC 4 cut(s) 379, 735, 3667, 3904
BbvI GCAGC 5 cut(s) 2259, 2358, 2562, 3432, 4200
BceAI ACGGC 2 cut(s) 1559, 4212
BciT130I CCWGG 4 cut(s) 89, 627, 1188, 3782
BclI TGATCA 2 cut(s) 36, 2614
BcoDI GTCTC 5 cut(s) 293, 889, 1665, 1690, 1985
BfmI CTRYAG 2 cut(s) 2111, 2339
BfrI CTTAAG 2 cut(s) 2260, 3602
BglII AGATCT 2 cut(s) 1552, 1606
BisI GCNGC 8 cut(s) 1498, 2248, 2372, 2576, 2947, 3446, 4140, 4189
BlsI GCNGC 8 cut(s) 1499, 2249, 2373, 2577, 2948, 3447, 4141, 4190
BmcAI AGTACT 1 cut(s) 4256
Bme1390I CCNGG 4 cut(s) 89, 627, 1188, 3782
Bme18I GGWCC 3 cut(s) 623, 1854, 3073
BmeT110I CYCGRG 1 cut(s) 2330
BmgT120I GGNCC 4 cut(s) 623, 1854, 3073, 3998
BmiI GGNNCC 4 cut(s) 1602, 1855, 2850, 2910
BmrFI CCNGG 4 cut(s) 89, 627, 1188, 3782
BmrI ACTGGG 1 cut(s) 3576
BmsI GCATC 8 cut(s) 72, 312, 1116, 1509, 1948, 3100, 3506, 4263
BmuI ACTGGG 1 cut(s) 3576
BoxI GACNNNNGTC 1 cut(s) 2486
BpiI GAAGAC 5 cut(s) 30, 765, 1299, 2163, 2387
BplI GAGNNNNNCTC 2 cut(s) 2217, 2249
BpmI CTGGAG 7 cut(s) 1569, 1856, 2066, 2927, 3109, 4365, 4499
BpuEI CTTGAG 6 cut(s) 422, 767, 1337, 2221, 3326, 3350
BsaAI YACGTR 2 cut(s) 2778, 4499
BsaBI GATNNNNATC 1 cut(s) 576
BsaJI CCNNGG 8 cut(s) 626, 792, 2128, 3066, 3267, 3941, 4072, 4409
BsaWI WCCGGW 4 cut(s) 1376, 2467, 2845, 3816
Bsc4I CCNNNNNNNGG 8 cut(s) 917, 1664, 2265, 2474, 2599, 3011, 3186, 4343
Bse1I ACTGG 7 cut(s) 1708, 1900, 2931, 3095, 3571, 4348, 4516
Bse3DI GCAATG 3 cut(s) 2541, 3714, 4376
Bse8I GATNNNNATC 1 cut(s) 576
BseAI TCCGGA 1 cut(s) 1376
BseBI CCWGG 4 cut(s) 89, 627, 1188, 3782
BseDI CCNNGG 8 cut(s) 626, 792, 2128, 3066, 3267, 3941, 4072, 4409
BseJI GATNNNNATC 1 cut(s) 576
BseLI CCNNNNNNNGG 8 cut(s) 917, 1664, 2265, 2474, 2599, 3011, 3186, 4343
BseMI GCAATG 3 cut(s) 2541, 3714, 4376
BseNI ACTGG 7 cut(s) 1708, 1900, 2931, 3095, 3571, 4348, 4516
BseSI GKGCMC 2 cut(s) 3904, 4435
BseXI GCAGC 5 cut(s) 2259, 2358, 2562, 3432, 4200
Bsh1236I CGCG 6 cut(s) 992, 2639, 2795, 3661, 4142, 4339
BshFI GGCC 6 cut(s) 819, 2765, 2946, 3616, 3999, 4408
BsiHKAI GWGCWC 4 cut(s) 379, 735, 3667, 3904
BsiHKCI CYCGRG 1 cut(s) 2330
BsiSI CCGG 4 cut(s) 1377, 2468, 2846, 3817
BslFI GGGAC 5 cut(s) 1867, 2937, 3389, 3531, 3577
BslI CCNNNNNNNGG 8 cut(s) 917, 1664, 2265, 2474, 2599, 3011, 3186, 4343
BsmAI GTCTC 5 cut(s) 293, 889, 1665, 1690, 1985
BsmBI CGTCTC 1 cut(s) 1665
BsmFI GGGAC 5 cut(s) 1867, 2937, 3389, 3531, 3577
BsmI GAATGC 2 cut(s) 709, 1722
BsnI GGCC 6 cut(s) 819, 2765, 2946, 3616, 3999, 4408
BsoBI CYCGRG 1 cut(s) 2330
Bsp1286I GDGCHC 5 cut(s) 379, 735, 3667, 3904, 4435
Bsp13I TCCGGA 1 cut(s) 1376
Bsp1407I TGTACA 1 cut(s) 496
Bsp19I CCATGG 2 cut(s) 3066, 4409
BspACI CCGC 9 cut(s) 72, 1498, 2637, 2947, 3139, 3204, 3659, 4140, 4337
BspANI GGCC 6 cut(s) 819, 2765, 2946, 3616, 3999, 4408
BspEI TCCGGA 1 cut(s) 1376
BspFNI CGCG 6 cut(s) 992, 2639, 2795, 3661, 4142, 4339
BspLI GGNNCC 4 cut(s) 1602, 1855, 2850, 2910
BspMAI CTGCAG 1 cut(s) 2343
BspQI GCTCTTC 1 cut(s) 1176
BspTI CTTAAG 2 cut(s) 2260, 3602
BsrBI CCGCTC 1 cut(s) 72
BsrDI GCAATG 3 cut(s) 2541, 3714, 4376
BsrGI TGTACA 1 cut(s) 496
BsrI ACTGG 7 cut(s) 1708, 1900, 2931, 3095, 3571, 4348, 4516
BssECI CCNNGG 8 cut(s) 626, 792, 2128, 3066, 3267, 3941, 4072, 4409
BssNAI GTATAC 1 cut(s) 694
BssT1I CCWWGG 5 cut(s) 792, 3066, 3267, 4072, 4409
Bst1107I GTATAC 1 cut(s) 694
Bst2UI CCWGG 4 cut(s) 89, 627, 1188, 3782
Bst4CI ACNGT 7 cut(s) 264, 1058, 1410, 2115, 3031, 3350, 4504
Bst6I CTCTTC 2 cut(s) 887, 1176
BstAFI CTTAAG 2 cut(s) 2260, 3602
BstAUI TGTACA 1 cut(s) 496
BstBAI YACGTR 2 cut(s) 2778, 4499
BstC8I GCNNGC 7 cut(s) 1487, 1538, 1832, 2973, 3227, 3663, 3824
BstDSI CCRYGG 3 cut(s) 3066, 3941, 4409
BstENI CCTNNNNNAGG 2 cut(s) 915, 2263
BstFNI CGCG 6 cut(s) 992, 2639, 2795, 3661, 4142, 4339
BstMAI GTCTC 5 cut(s) 293, 889, 1665, 1690, 1985
BstMWI GCNNNNNNNGC 8 cut(s) 69, 680, 2368, 2380, 2636, 2908, 3494, 3613
BstNI CCWGG 4 cut(s) 89, 627, 1188, 3782
BstNSI RCATGY 4 cut(s) 1489, 1540, 3611, 3826
BstPAI GACNNNNGTC 1 cut(s) 2486
BstSCI CCNGG 4 cut(s) 87, 625, 1186, 3780
BstSFI CTRYAG 2 cut(s) 2111, 2339
BstSLI GKGCMC 2 cut(s) 3904, 4435
BstSNI TACGTA 1 cut(s) 4499
BstUI CGCG 6 cut(s) 992, 2639, 2795, 3661, 4142, 4339
BstV1I GCAGC 5 cut(s) 2259, 2358, 2562, 3432, 4200
BstV2I GAAGAC 5 cut(s) 30, 765, 1299, 2163, 2387
BstX2I RGATCY 7 cut(s) 845, 1552, 1606, 1667, 1912, 2848, 4509
BstXI CCANNNNNNTGG 2 cut(s) 1235, 3083
BstYI RGATCY 7 cut(s) 845, 1552, 1606, 1667, 1912, 2848, 4509
BstZ17I GTATAC 1 cut(s) 694
BsuRI GGCC 6 cut(s) 819, 2765, 2946, 3616, 3999, 4408
BtgI CCRYGG 3 cut(s) 3066, 3941, 4409
BtgZI GCGATG 1 cut(s) 973
BtsI GCAGTG 3 cut(s) 1734, 4191, 4425
BtsIMutI CAGTG 8 cut(s) 195, 1593, 1734, 2426, 2940, 3736, 4191, 4425
Cac8I GCNNGC 7 cut(s) 1487, 1538, 1832, 2973, 3227, 3663, 3824
CaiI CAGNNNCTG 1 cut(s) 2119
Cfr13I GGNCC 4 cut(s) 623, 1854, 3073, 3998
CseI GACGC 2 cut(s) 113, 2891
Csp6I GTAC 9 cut(s) 19, 441, 497, 612, 2421, 2676, 3778, 4255, 4500
CviQI GTAC 9 cut(s) 19, 441, 497, 612, 2421, 2676, 3778, 4255, 4500
DraI TTTAAA 2 cut(s) 1003, 4287
EaeI YGGCCR 1 cut(s) 2763
Eam1104I CTCTTC 2 cut(s) 887, 1176
EarI CTCTTC 2 cut(s) 887, 1176
EciI GGCGGA 1 cut(s) 3154
Ecl136II GAGCTC 2 cut(s) 377, 733
Eco105I TACGTA 1 cut(s) 4499
Eco130I CCWWGG 5 cut(s) 792, 3066, 3267, 4072, 4409
Eco24I GRGCYC 2 cut(s) 379, 735
Eco47I GGWCC 3 cut(s) 623, 1854, 3073
Eco53kI GAGCTC 2 cut(s) 377, 733
Eco57I CTGAAG 6 cut(s) 135, 177, 741, 1694, 1805, 2490
Eco72I CACGTG 1 cut(s) 2778
Eco88I CYCGRG 1 cut(s) 2330
EcoICRI GAGCTC 2 cut(s) 377, 733
EcoNI CCTNNNNNAGG 2 cut(s) 915, 2263
EcoRI GAATTC 2 cut(s) 3087, 3254
EcoRII CCWGG 4 cut(s) 87, 625, 1186, 3780
EcoT14I CCWWGG 5 cut(s) 792, 3066, 3267, 4072, 4409
EcoT22I ATGCAT 4 cut(s) 709, 1131, 1491, 4278
EcoT38I GRGCYC 2 cut(s) 379, 735
ErhI CCWWGG 5 cut(s) 792, 3066, 3267, 4072, 4409
Esp3I CGTCTC 1 cut(s) 1665
FaqI GGGAC 5 cut(s) 1867, 2937, 3389, 3531, 3577
FauI CCCGC 1 cut(s) 2644
FauNDI CATATG 2 cut(s) 2587, 2914
FbaI TGATCA 2 cut(s) 36, 2614
FblI GTMKAC 3 cut(s) 693, 2356, 4441
Fnu4HI GCNGC 8 cut(s) 1498, 2248, 2372, 2576, 2947, 3446, 4140, 4189
FriOI GRGCYC 2 cut(s) 379, 735
Fsp4HI GCNGC 8 cut(s) 1498, 2248, 2372, 2576, 2947, 3446, 4140, 4189
GluI GCNGC 8 cut(s) 1498, 2248, 2372, 2576, 2947, 3446, 4140, 4189
GsuI CTGGAG 7 cut(s) 1569, 1856, 2066, 2927, 3109, 4365, 4499
HaeIII GGCC 6 cut(s) 819, 2765, 2946, 3616, 3999, 4408
HapII CCGG 4 cut(s) 1377, 2468, 2846, 3817
HgaI GACGC 2 cut(s) 113, 2891
HindIII AAGCTT 5 cut(s) 723, 1639, 1828, 2051, 2381
HpaII CCGG 4 cut(s) 1377, 2468, 2846, 3817
HphI GGTGA 8 cut(s) 46, 242, 401, 712, 1261, 1646, 2457, 3418
HpyCH4III ACNGT 7 cut(s) 264, 1058, 1410, 2115, 3031, 3350, 4504
HpyCH4IV ACGT 5 cut(s) 1195, 2403, 2777, 4176, 4498
HpyF10VI GCNNNNNNNGC 8 cut(s) 69, 680, 2368, 2380, 2636, 2908, 3494, 3613
HpySE526I ACGT 5 cut(s) 1195, 2403, 2777, 4176, 4498
Kpn2I TCCGGA 1 cut(s) 1376
Ksp22I TGATCA 2 cut(s) 36, 2614
LguI GCTCTTC 1 cut(s) 1176
LmnI GCTCC 9 cut(s) 680, 1533, 1837, 2003, 2191, 2225, 2367, 2908, 4201
Lsp1109I GCAGC 5 cut(s) 2259, 2358, 2562, 3432, 4200
LweI GCATC 8 cut(s) 72, 312, 1116, 1509, 1948, 3100, 3506, 4263
MaeII ACGT 5 cut(s) 1195, 2403, 2777, 4176, 4498
MaeIII GTNAC 9 cut(s) 1046, 1099, 1249, 1404, 2479, 2581, 3553, 3966, 4387
MbiI CCGCTC 1 cut(s) 72
MfeI CAATTG 4 cut(s) 590, 1311, 1881, 2366
MflI RGATCY 7 cut(s) 845, 1552, 1606, 1667, 1912, 2848, 4509
MhlI GDGCHC 5 cut(s) 379, 735, 3667, 3904, 4435
MlyI GAGTC 6 cut(s) 1771, 2480, 2511, 3227, 4229, 4300
MmeI TCCRAC 7 cut(s) 748, 1220, 1310, 1890, 2520, 4117, 4182
Mph1103I ATGCAT 4 cut(s) 709, 1131, 1491, 4278
MroI TCCGGA 1 cut(s) 1376
MroXI GAANNNNTTC 7 cut(s) 749, 777, 1161, 1749, 3258, 4052, 4109
MslI CAYNNNNRTG 8 cut(s) 255, 509, 1070, 1848, 3287, 3348, 3729, 4428
MspCI CTTAAG 2 cut(s) 2260, 3602
MspI CCGG 4 cut(s) 1377, 2468, 2846, 3817
MspR9I CCNGG 4 cut(s) 89, 627, 1188, 3782
MunI CAATTG 4 cut(s) 590, 1311, 1881, 2366
Mva1269I GAATGC 2 cut(s) 709, 1722
MvaI CCWGG 4 cut(s) 89, 627, 1188, 3782
MvnI CGCG 6 cut(s) 992, 2639, 2795, 3661, 4142, 4339
MwoI GCNNNNNNNGC 8 cut(s) 69, 680, 2368, 2380, 2636, 2908, 3494, 3613
NcoI CCATGG 2 cut(s) 3066, 4409
NdeI CATATG 2 cut(s) 2587, 2914
NlaIV GGNNCC 4 cut(s) 1602, 1855, 2850, 2910
NmeAIII GCCGAG 1 cut(s) 2153
NmuCI GTSAC 5 cut(s) 1046, 1249, 2479, 2581, 3966
NsiI ATGCAT 4 cut(s) 709, 1131, 1491, 4278
NspI RCATGY 4 cut(s) 1489, 1540, 3611, 3826
OliI CACNNNNGTG 1 cut(s) 3287
PaeI GCATGC 3 cut(s) 1489, 1540, 3826
PaeR7I CTCGAG 1 cut(s) 2330
PciSI GCTCTTC 1 cut(s) 1176
PctI GAATGC 2 cut(s) 709, 1722
PdmI GAANNNNTTC 7 cut(s) 749, 777, 1161, 1749, 3258, 4052, 4109
PkrI GCNGC 8 cut(s) 1499, 2249, 2373, 2577, 2948, 3447, 4141, 4190
PleI GAGTC 6 cut(s) 1770, 2479, 2511, 3226, 4229, 4299
PmaCI CACGTG 1 cut(s) 2778
PmlI CACGTG 1 cut(s) 2778
PpsI GAGTC 6 cut(s) 1770, 2479, 2511, 3226, 4229, 4299
Ppu21I YACGTR 2 cut(s) 2778, 4499
PshAI GACNNNNGTC 1 cut(s) 2486
Psp124BI GAGCTC 2 cut(s) 379, 735
Psp1406I AACGTT 1 cut(s) 4176
Psp6I CCWGG 4 cut(s) 87, 625, 1186, 3780
PspCI CACGTG 1 cut(s) 2778
PspGI CCWGG 4 cut(s) 87, 625, 1186, 3780
PspN4I GGNNCC 4 cut(s) 1602, 1855, 2850, 2910
PspPI GGNCC 4 cut(s) 623, 1854, 3073, 3998
PspXI VCTCGAGB 1 cut(s) 2330
PstI CTGCAG 1 cut(s) 2343
PstNI CAGNNNCTG 1 cut(s) 2119
PsuI RGATCY 7 cut(s) 845, 1552, 1606, 1667, 1912, 2848, 4509
RsaI GTAC 9 cut(s) 20, 442, 498, 613, 2422, 2677, 3779, 4256, 4501
RsaNI GTAC 9 cut(s) 19, 441, 497, 612, 2421, 2676, 3778, 4255, 4500
RseI CAYNNNNRTG 8 cut(s) 255, 509, 1070, 1848, 3287, 3348, 3729, 4428
SacI GAGCTC 2 cut(s) 379, 735
SapI GCTCTTC 1 cut(s) 1176
SatI GCNGC 8 cut(s) 1498, 2248, 2372, 2576, 2947, 3446, 4140, 4189
Sau96I GGNCC 4 cut(s) 623, 1854, 3073, 3998
ScaI AGTACT 1 cut(s) 4256
SchI GAGTC 6 cut(s) 1771, 2480, 2511, 3227, 4229, 4300
ScrFI CCNGG 4 cut(s) 89, 627, 1188, 3782
SduI GDGCHC 5 cut(s) 379, 735, 3667, 3904, 4435
SfaNI GCATC 8 cut(s) 72, 312, 1116, 1509, 1948, 3100, 3506, 4263
SfcI CTRYAG 2 cut(s) 2111, 2339
Sfr274I CTCGAG 1 cut(s) 2330
SinI GGWCC 3 cut(s) 623, 1854, 3073
SlaI CTCGAG 1 cut(s) 2330
SmiMI CAYNNNNRTG 8 cut(s) 255, 509, 1070, 1848, 3287, 3348, 3729, 4428
SmlI CTYRAG 9 cut(s) 401, 782, 1352, 2200, 2260, 2330, 3305, 3329, 3602
SmoI CTYRAG 9 cut(s) 401, 782, 1352, 2200, 2260, 2330, 3305, 3329, 3602
SnaBI TACGTA 1 cut(s) 4499
SphI GCATGC 3 cut(s) 1489, 1540, 3826
SsiI CCGC 9 cut(s) 72, 1498, 2637, 2947, 3139, 3204, 3659, 4140, 4337
SspI AATATT 2 cut(s) 1758, 3848
SstI GAGCTC 2 cut(s) 379, 735
StyD4I CCNGG 4 cut(s) 87, 625, 1186, 3780
StyI CCWWGG 5 cut(s) 792, 3066, 3267, 4072, 4409
TaaI ACNGT 7 cut(s) 264, 1058, 1410, 2115, 3031, 3350, 4504
TaiI ACGT 5 cut(s) 1198, 2406, 2780, 4179, 4501
TaqI TCGA 8 cut(s) 66, 1803, 1816, 2331, 2490, 2747, 4113, 4269
TatI WGTACW 4 cut(s) 440, 496, 2420, 4254
TauI GCSGC 3 cut(s) 1500, 2949, 4142
TscAI CASTG 8 cut(s) 202, 1600, 1741, 2433, 2940, 3736, 4198, 4425
TseFI GTSAC 5 cut(s) 1046, 1249, 2479, 2581, 3966
TseI GCWGC 5 cut(s) 2247, 2371, 2575, 3445, 4188
Tsp45I GTSAC 5 cut(s) 1046, 1249, 2479, 2581, 3966
TspGWI ACGGA 1 cut(s) 2447
TspRI CASTG 8 cut(s) 202, 1600, 1741, 2433, 2940, 3736, 4198, 4425
Vha464I CTTAAG 2 cut(s) 2260, 3602
VneI GTGCAC 1 cut(s) 3900
VpaK11BI GGWCC 3 cut(s) 623, 1854, 3073
XagI CCTNNNNNAGG 2 cut(s) 915, 2263
XapI RAATTY 6 cut(s) 797, 2669, 2697, 3087, 3254, 3421
XbaI TCTAGA 3 cut(s) 1435, 2569, 2689
XceI RCATGY 4 cut(s) 1489, 1540, 3611, 3826
XcmI CCANNNNNNNNNTGG 1 cut(s) 3064
XhoI CTCGAG 1 cut(s) 2330
XmiI GTMKAC 3 cut(s) 693, 2356, 4441
XmnI GAANNNNTTC 7 cut(s) 749, 777, 1161, 1749, 3258, 4052, 4109
ZrmI AGTACT 1 cut(s) 4256
Zsp2I ATGCAT 4 cut(s) 709, 1131, 1491, 4278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.