resistance protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 3 cut(s) | 579, 2558, 2790 |
| Acc36I | ACCTGC | 2 cut(s) | 753, 3174 |
| AccB1I | GGYRCC | 2 cut(s) | 2025, 4454 |
| AccBSI | CCGCTC | 1 cut(s) | 152 |
| AccI | GTMKAC | 3 cut(s) | 579, 2177, 5017 |
| AccII | CGCG | 5 cut(s) | 737, 1589, 3743, 4152, 4915 |
| AccIII | TCCGGA | 1 cut(s) | 1510 |
| AciI | CCGC | 7 cut(s) | 121, 150, 442, 719, 758, 3538, 4913 |
| AclI | AACGTT | 1 cut(s) | 1574 |
| AclWI | GGATC | 8 cut(s) | 371, 1182, 1450, 1623, 2272, 3443, 3456, 3556 |
| AcoI | YGGCCR | 3 cut(s) | 3363, 3535, 4956 |
| AcuI | CTGAAG | 6 cut(s) | 513, 792, 1338, 2291, 2402, 3869 |
| AcvI | CACGTG | 1 cut(s) | 2992 |
| AcyI | GRCGYC | 4 cut(s) | 576, 680, 2555, 2787 |
| AdeI | CACNNNGTG | 1 cut(s) | 3605 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 620, 1109, 1514, 2261, 2853, 3509, 4465 |
| AflII | CTTAAG | 1 cut(s) | 2848 |
| AflIII | ACRYGT | 1 cut(s) | 2991 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 350 |
| AhlI | ACTAGT | 1 cut(s) | 3841 |
| AjiI | CACGTC | 2 cut(s) | 3522, 4165 |
| AjnI | CCWGG | 3 cut(s) | 1783, 3934, 4958 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 1347, 1379, 1562, 1594 |
| AleI | CACNNNNGTG | 1 cut(s) | 3842 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1434, 1466 |
| Alw21I | GWGCWC | 4 cut(s) | 312, 411, 1332, 2919 |
| AlwI | GGATC | 8 cut(s) | 371, 1182, 1450, 1623, 2272, 3443, 3456, 3556 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 2707 |
| Ama87I | CYCGRG | 5 cut(s) | 2606, 2918, 3061, 4215, 4877 |
| Aor13HI | TCCGGA | 1 cut(s) | 1510 |
| AoxI | GGCC | 5 cut(s) | 1414, 3363, 3535, 4169, 4956 |
| ApeKI | GCWGC | 6 cut(s) | 2094, 2835, 2947, 2959, 3497, 4000 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 572, 604 |
| Asp700I | GAANNNNTTC | 5 cut(s) | 1346, 1758, 2346, 4613, 4670 |
| AspLEI | GCGC | 8 cut(s) | 733, 3232, 3401, 3502, 4075, 4114, 4521, 4917 |
| AspS9I | GGNCC | 6 cut(s) | 218, 611, 742, 2442, 3163, 3667 |
| AsuC2I | CCSGG | 1 cut(s) | 4337 |
| AsuHPI | GGTGA | 5 cut(s) | 857, 1309, 2243, 3640, 5064 |
| AsuNHI | GCTAGC | 1 cut(s) | 3287 |
| AvaI | CYCGRG | 5 cut(s) | 2606, 2918, 3061, 4215, 4877 |
| AvaII | GGWCC | 6 cut(s) | 218, 611, 742, 2442, 3163, 3667 |
| BaeGI | GKGCMC | 4 cut(s) | 2030, 4164, 4459, 5011 |
| BalI | TGGCCA | 1 cut(s) | 4958 |
| BamHI | GGATCC | 1 cut(s) | 3448 |
| BanI | GGYRCC | 2 cut(s) | 2025, 4454 |
| BanII | GRGCYC | 3 cut(s) | 312, 1332, 2919 |
| BbrPI | CACGTG | 1 cut(s) | 2992 |
| BbsI | GAAGAC | 3 cut(s) | 1362, 1896, 2975 |
| Bbv12I | GWGCWC | 4 cut(s) | 312, 411, 1332, 2919 |
| BbvI | GCAGC | 6 cut(s) | 2106, 2847, 2946, 2959, 3484, 3987 |
| BceAI | ACGGC | 2 cut(s) | 2156, 4831 |
| BcgI | CGANNNNNNTGC | 8 cut(s) | 273, 307, 727, 761, 3686, 3720, 4712, 4746 |
| BciT130I | CCWGG | 3 cut(s) | 1785, 3936, 4960 |
| BclI | TGATCA | 1 cut(s) | 3205 |
| BcnI | CCSGG | 1 cut(s) | 4337 |
| BcuI | ACTAGT | 1 cut(s) | 3841 |
| BfmI | CTRYAG | 4 cut(s) | 2699, 3072, 3279, 4644 |
| BfrI | CTTAAG | 1 cut(s) | 2848 |
| BfuAI | ACCTGC | 2 cut(s) | 753, 3174 |
| BglII | AGATCT | 6 cut(s) | 16, 2149, 2203, 3320, 3899, 5041 |
| BisI | GCNGC | 7 cut(s) | 2095, 2836, 2948, 2960, 3498, 3538, 4001 |
| BlpI | GCTNAGC | 1 cut(s) | 4990 |
| BlsI | GCNGC | 7 cut(s) | 2096, 2837, 2949, 2961, 3499, 3539, 4002 |
| BmcAI | AGTACT | 1 cut(s) | 4753 |
| Bme1390I | CCNGG | 4 cut(s) | 1785, 3936, 4337, 4960 |
| Bme18I | GGWCC | 6 cut(s) | 218, 611, 742, 2442, 3163, 3667 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 350 |
| BmeT110I | CYCGRG | 5 cut(s) | 2606, 2918, 3061, 4215, 4877 |
| BmgBI | CACGTC | 2 cut(s) | 3522, 4165 |
| BmgT120I | GGNCC | 6 cut(s) | 218, 611, 742, 2442, 3163, 3667 |
| BmiI | GGNNCC | 8 cut(s) | 456, 613, 2027, 2199, 2443, 3450, 4322, 4456 |
| BmrFI | CCNGG | 4 cut(s) | 1785, 3936, 4337, 4960 |
| BmrI | ACTGGG | 2 cut(s) | 774, 4131 |
| BmtI | GCTAGC | 1 cut(s) | 3291 |
| BmuI | ACTGGG | 2 cut(s) | 774, 4131 |
| BoxI | GACNNNNGTC | 2 cut(s) | 3074, 4563 |
| BpiI | GAAGAC | 3 cut(s) | 1362, 1896, 2975 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 2805, 2837 |
| BpmI | CTGGAG | 5 cut(s) | 1806, 2166, 2444, 2654, 3078 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 4990 |
| BpuEI | CTTGAG | 5 cut(s) | 140, 218, 1934, 2953, 3881 |
| BpuMI | CCSGG | 1 cut(s) | 4337 |
| BsaAI | YACGTR | 2 cut(s) | 2992, 4856 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1173 |
| BsaHI | GRCGYC | 4 cut(s) | 576, 680, 2555, 2787 |
| BsaWI | WCCGGW | 3 cut(s) | 1510, 2059, 3445 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 752, 782, 1460, 1490 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 620, 1109, 1514, 2261, 2853, 3509, 4465 |
| Bse1I | ACTGG | 5 cut(s) | 769, 2488, 4126, 4372, 4556 |
| Bse3DI | GCAATG | 2 cut(s) | 700, 4952 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1173 |
| BseAI | TCCGGA | 1 cut(s) | 1510 |
| BseBI | CCWGG | 3 cut(s) | 1785, 3936, 4960 |
| BseJI | GATNNNNATC | 1 cut(s) | 1173 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 620, 1109, 1514, 2261, 2853, 3509, 4465 |
| BseMI | GCAATG | 2 cut(s) | 700, 4952 |
| BseNI | ACTGG | 5 cut(s) | 769, 2488, 4126, 4372, 4556 |
| BseRI | GAGGAG | 2 cut(s) | 51, 54 |
| BseSI | GKGCMC | 4 cut(s) | 2030, 4164, 4459, 5011 |
| BseXI | GCAGC | 6 cut(s) | 2106, 2847, 2946, 2959, 3484, 3987 |
| BsgI | GTGCAG | 2 cut(s) | 731, 1875 |
| Bsh1236I | CGCG | 5 cut(s) | 737, 1589, 3743, 4152, 4915 |
| Bsh1285I | CGRYCG | 1 cut(s) | 4448 |
| BshFI | GGCC | 5 cut(s) | 1416, 3365, 3537, 4171, 4958 |
| BshNI | GGYRCC | 2 cut(s) | 2025, 4454 |
| BsiEI | CGRYCG | 1 cut(s) | 4448 |
| BsiHKAI | GWGCWC | 4 cut(s) | 312, 411, 1332, 2919 |
| BsiHKCI | CYCGRG | 5 cut(s) | 2606, 2918, 3061, 4215, 4877 |
| BsiSI | CCGG | 4 cut(s) | 1511, 2060, 3446, 4336 |
| BslFI | GGGAC | 2 cut(s) | 2455, 4702 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 620, 1109, 1514, 2261, 2853, 3509, 4465 |
| BsmBI | CGTCTC | 2 cut(s) | 2262, 2630 |
| BsmFI | GGGAC | 2 cut(s) | 2455, 4702 |
| BsmI | GAATGC | 1 cut(s) | 1306 |
| BsnI | GGCC | 5 cut(s) | 1416, 3365, 3537, 4171, 4958 |
| BsoBI | CYCGRG | 5 cut(s) | 2606, 2918, 3061, 4215, 4877 |
| Bsp1286I | GDGCHC | 8 cut(s) | 312, 411, 1332, 2030, 2919, 4164, 4459, 5011 |
| Bsp13I | TCCGGA | 1 cut(s) | 1510 |
| Bsp1407I | TGTACA | 3 cut(s) | 796, 1093, 1290 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 4990 |
| Bsp19I | CCATGG | 2 cut(s) | 3660, 4263 |
| Bsp68I | TCGCGA | 1 cut(s) | 737 |
| BspACI | CCGC | 7 cut(s) | 121, 150, 442, 719, 758, 3538, 4913 |
| BspANI | GGCC | 5 cut(s) | 1416, 3365, 3537, 4171, 4958 |
| BspEI | TCCGGA | 1 cut(s) | 1510 |
| BspFNI | CGCG | 5 cut(s) | 737, 1589, 3743, 4152, 4915 |
| BspHI | TCATGA | 1 cut(s) | 4830 |
| BspLI | GGNNCC | 8 cut(s) | 456, 613, 2027, 2199, 2443, 3450, 4322, 4456 |
| BspMAI | CTGCAG | 1 cut(s) | 4648 |
| BspMI | ACCTGC | 2 cut(s) | 753, 3174 |
| BspOI | GCTAGC | 1 cut(s) | 3291 |
| BspPI | GGATC | 8 cut(s) | 371, 1182, 1450, 1623, 2272, 3443, 3456, 3556 |
| BspT107I | GGYRCC | 2 cut(s) | 2025, 4454 |
| BspTI | CTTAAG | 1 cut(s) | 2848 |
| BsrBI | CCGCTC | 1 cut(s) | 152 |
| BsrDI | GCAATG | 2 cut(s) | 700, 4952 |
| BsrGI | TGTACA | 3 cut(s) | 796, 1093, 1290 |
| BsrI | ACTGG | 5 cut(s) | 769, 2488, 4126, 4372, 4556 |
| BssNI | GRCGYC | 4 cut(s) | 576, 680, 2555, 2787 |
| BssT1I | CCWWGG | 6 cut(s) | 1389, 3544, 3660, 3822, 4263, 4633 |
| Bst2UI | CCWGG | 3 cut(s) | 1785, 3936, 4960 |
| Bst6I | CTCTTC | 3 cut(s) | 34, 444, 1484 |
| BstACI | GRCGYC | 4 cut(s) | 576, 680, 2555, 2787 |
| BstAFI | CTTAAG | 1 cut(s) | 2848 |
| BstAUI | TGTACA | 3 cut(s) | 796, 1093, 1290 |
| BstBAI | YACGTR | 2 cut(s) | 2992, 4856 |
| BstDSI | CCRYGG | 3 cut(s) | 3660, 4263, 4496 |
| BstENI | CCTNNNNNAGG | 3 cut(s) | 1512, 2851, 3507 |
| BstFNI | CGCG | 5 cut(s) | 737, 1589, 3743, 4152, 4915 |
| BstHHI | GCGC | 8 cut(s) | 733, 3232, 3401, 3502, 4075, 4114, 4521, 4917 |
| BstMCI | CGRYCG | 1 cut(s) | 4448 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 439, 529, 1277, 1853, 2956, 4049, 4168, 4879 |
| BstNI | CCWGG | 3 cut(s) | 1785, 3936, 4960 |
| BstNSI | RCATGY | 4 cut(s) | 1275, 2137, 3173, 4381 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 3074, 4563 |
| BstSCI | CCNGG | 4 cut(s) | 1783, 3934, 4335, 4958 |
| BstSFI | CTRYAG | 4 cut(s) | 2699, 3072, 3279, 4644 |
| BstSLI | GKGCMC | 4 cut(s) | 2030, 4164, 4459, 5011 |
| BstSNI | TACGTA | 1 cut(s) | 4856 |
| BstUI | CGCG | 5 cut(s) | 737, 1589, 3743, 4152, 4915 |
| BstV1I | GCAGC | 6 cut(s) | 2106, 2847, 2946, 2959, 3484, 3987 |
| BstV2I | GAAGAC | 3 cut(s) | 1362, 1896, 2975 |
| BstX2I | RGATCY | 8 cut(s) | 16, 2149, 2203, 2264, 3320, 3448, 3899, 5041 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 3677 |
| BstYI | RGATCY | 8 cut(s) | 16, 2149, 2203, 2264, 3320, 3448, 3899, 5041 |
| BsuRI | GGCC | 5 cut(s) | 1416, 3365, 3537, 4171, 4958 |
| BtgI | CCRYGG | 3 cut(s) | 3660, 4263, 4496 |
| BtgZI | GCGATG | 3 cut(s) | 752, 1570, 3306 |
| BtrI | CACGTC | 2 cut(s) | 3522, 4165 |
| BtsI | GCAGTG | 2 cut(s) | 2331, 5001 |
| BtuMI | TCGCGA | 1 cut(s) | 737 |
| BveI | ACCTGC | 2 cut(s) | 753, 3174 |
| CaiI | CAGNNNCTG | 1 cut(s) | 2707 |
| CciI | TCATGA | 1 cut(s) | 4830 |
| CfoI | GCGC | 8 cut(s) | 733, 3232, 3401, 3502, 4075, 4114, 4521, 4917 |
| Cfr13I | GGNCC | 6 cut(s) | 218, 611, 742, 2442, 3163, 3667 |
| CseI | GACGC | 2 cut(s) | 669, 2647 |
| DraI | TTTAAA | 1 cut(s) | 1600 |
| DraIII | CACNNNGTG | 1 cut(s) | 3605 |
| DriI | GACNNNNNGTC | 1 cut(s) | 350 |
| EaeI | YGGCCR | 3 cut(s) | 3363, 3535, 4956 |
| Eam1104I | CTCTTC | 3 cut(s) | 34, 444, 1484 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 350 |
| EarI | CTCTTC | 3 cut(s) | 34, 444, 1484 |
| EciI | GGCGGA | 1 cut(s) | 773 |
| Ecl136II | GAGCTC | 3 cut(s) | 310, 1330, 2917 |
| Eco105I | TACGTA | 1 cut(s) | 4856 |
| Eco130I | CCWWGG | 6 cut(s) | 1389, 3544, 3660, 3822, 4263, 4633 |
| Eco24I | GRGCYC | 3 cut(s) | 312, 1332, 2919 |
| Eco32I | GATATC | 1 cut(s) | 3430 |
| Eco47I | GGWCC | 6 cut(s) | 218, 611, 742, 2442, 3163, 3667 |
| Eco53kI | GAGCTC | 3 cut(s) | 310, 1330, 2917 |
| Eco57I | CTGAAG | 6 cut(s) | 513, 792, 1338, 2291, 2402, 3869 |
| Eco72I | CACGTG | 1 cut(s) | 2992 |
| Eco88I | CYCGRG | 5 cut(s) | 2606, 2918, 3061, 4215, 4877 |
| EcoICRI | GAGCTC | 3 cut(s) | 310, 1330, 2917 |
| EcoNI | CCTNNNNNAGG | 3 cut(s) | 1512, 2851, 3507 |
| EcoO109I | RGGNCCY | 1 cut(s) | 611 |
| EcoRI | GAATTC | 3 cut(s) | 2319, 3049, 3681 |
| EcoRII | CCWGG | 3 cut(s) | 1783, 3934, 4958 |
| EcoRV | GATATC | 1 cut(s) | 3430 |
| EcoT14I | CCWWGG | 6 cut(s) | 1389, 3544, 3660, 3822, 4263, 4633 |
| EcoT22I | ATGCAT | 2 cut(s) | 1306, 1728 |
| EcoT38I | GRGCYC | 3 cut(s) | 312, 1332, 2919 |
| ErhI | CCWWGG | 6 cut(s) | 1389, 3544, 3660, 3822, 4263, 4633 |
| Esp3I | CGTCTC | 2 cut(s) | 2262, 2630 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1758, 1790, 4380, 4412 |
| FaqI | GGGAC | 2 cut(s) | 2455, 4702 |
| FauI | CCCGC | 1 cut(s) | 128 |
| FbaI | TGATCA | 1 cut(s) | 3205 |
| FblI | GTMKAC | 3 cut(s) | 579, 2177, 5017 |
| Fnu4HI | GCNGC | 7 cut(s) | 2095, 2836, 2948, 2960, 3498, 3538, 4001 |
| FriOI | GRGCYC | 3 cut(s) | 312, 1332, 2919 |
| Fsp4HI | GCNGC | 7 cut(s) | 2095, 2836, 2948, 2960, 3498, 3538, 4001 |
| GlaI | GCGC | 8 cut(s) | 732, 3231, 3400, 3501, 4074, 4113, 4520, 4916 |
| GluI | GCNGC | 7 cut(s) | 2095, 2836, 2948, 2960, 3498, 3538, 4001 |
| GsuI | CTGGAG | 5 cut(s) | 1806, 2166, 2444, 2654, 3078 |
| HaeIII | GGCC | 5 cut(s) | 1416, 3365, 3537, 4171, 4958 |
| HapII | CCGG | 4 cut(s) | 1511, 2060, 3446, 4336 |
| HgaI | GACGC | 2 cut(s) | 669, 2647 |
| HhaI | GCGC | 8 cut(s) | 733, 3232, 3401, 3502, 4075, 4114, 4521, 4917 |
| Hin1I | GRCGYC | 4 cut(s) | 576, 680, 2555, 2787 |
| Hin6I | GCGC | 8 cut(s) | 731, 3230, 3399, 3500, 4073, 4112, 4519, 4915 |
| HinP1I | GCGC | 8 cut(s) | 731, 3230, 3399, 3500, 4073, 4112, 4519, 4915 |
| HincII | GTYRAC | 3 cut(s) | 561, 580, 2178 |
| HindII | GTYRAC | 3 cut(s) | 561, 580, 2178 |
| HindIII | AAGCTT | 2 cut(s) | 1320, 2360 |
| HpaII | CCGG | 4 cut(s) | 1511, 2060, 3446, 4336 |
| HphI | GGTGA | 5 cut(s) | 857, 1309, 2243, 3640, 5064 |
| Hpy99I | CGWCG | 4 cut(s) | 581, 911, 1852, 3523 |
| HpyCH4IV | ACGT | 9 cut(s) | 576, 1574, 1792, 2555, 2787, 2991, 3521, 4164, 4855 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 439, 529, 1277, 1853, 2956, 4049, 4168, 4879 |
| HpySE526I | ACGT | 9 cut(s) | 576, 1574, 1792, 2555, 2787, 2991, 3521, 4164, 4855 |
| Hsp92I | GRCGYC | 4 cut(s) | 576, 680, 2555, 2787 |
| HspAI | GCGC | 8 cut(s) | 731, 3230, 3399, 3500, 4073, 4112, 4519, 4915 |
| Kpn2I | TCCGGA | 1 cut(s) | 1510 |
| Ksp22I | TGATCA | 1 cut(s) | 3205 |
| LmnI | GCTCC | 5 cut(s) | 2425, 2591, 2813, 4589, 4762 |
| Lsp1109I | GCAGC | 6 cut(s) | 2106, 2847, 2946, 2959, 3484, 3987 |
| MaeII | ACGT | 9 cut(s) | 576, 1574, 1792, 2555, 2787, 2991, 3521, 4164, 4855 |
| MaeIII | GTNAC | 5 cut(s) | 319, 2001, 3067, 3172, 4577 |
| MbiI | CCGCTC | 1 cut(s) | 152 |
| MfeI | CAATTG | 6 cut(s) | 213, 1041, 1187, 1908, 2469, 2954 |
| MflI | RGATCY | 8 cut(s) | 16, 2149, 2203, 2264, 3320, 3448, 3899, 5041 |
| MhlI | GDGCHC | 8 cut(s) | 312, 411, 1332, 2030, 2919, 4164, 4459, 5011 |
| MlsI | TGGCCA | 1 cut(s) | 4958 |
| MluNI | TGGCCA | 1 cut(s) | 4958 |
| MlyI | GAGTC | 8 cut(s) | 613, 2173, 2917, 3053, 3104, 3872, 3959, 4790 |
| MmeI | TCCRAC | 4 cut(s) | 1345, 2080, 2838, 4678 |
| Mox20I | TGGCCA | 1 cut(s) | 4958 |
| Mph1103I | ATGCAT | 2 cut(s) | 1306, 1728 |
| MroI | TCCGGA | 1 cut(s) | 1510 |
| MroXI | GAANNNNTTC | 5 cut(s) | 1346, 1758, 2346, 4613, 4670 |
| MscI | TGGCCA | 1 cut(s) | 4958 |
| MslI | CAYNNNNRTG | 6 cut(s) | 870, 2436, 2994, 3842, 4842, 5004 |
| Msp20I | TGGCCA | 1 cut(s) | 4958 |
| MspA1I | CMGCKG | 1 cut(s) | 4792 |
| MspCI | CTTAAG | 1 cut(s) | 2848 |
| MspI | CCGG | 4 cut(s) | 1511, 2060, 3446, 4336 |
| MspR9I | CCNGG | 4 cut(s) | 1785, 3936, 4337, 4960 |
| MunI | CAATTG | 6 cut(s) | 213, 1041, 1187, 1908, 2469, 2954 |
| Mva1269I | GAATGC | 1 cut(s) | 1306 |
| MvaI | CCWGG | 3 cut(s) | 1785, 3936, 4960 |
| MvnI | CGCG | 5 cut(s) | 737, 1589, 3743, 4152, 4915 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 439, 529, 1277, 1853, 2956, 4049, 4168, 4879 |
| NciI | CCSGG | 1 cut(s) | 4337 |
| NcoI | CCATGG | 2 cut(s) | 3660, 4263 |
| NheI | GCTAGC | 1 cut(s) | 3287 |
| NlaIV | GGNNCC | 8 cut(s) | 456, 613, 2027, 2199, 2443, 3450, 4322, 4456 |
| NmeAIII | GCCGAG | 1 cut(s) | 2741 |
| NmuCI | GTSAC | 3 cut(s) | 319, 3067, 3172 |
| NruI | TCGCGA | 1 cut(s) | 737 |
| NsiI | ATGCAT | 2 cut(s) | 1306, 1728 |
| NspI | RCATGY | 4 cut(s) | 1275, 2137, 3173, 4381 |
| OliI | CACNNNNGTG | 1 cut(s) | 3842 |
| PaeI | GCATGC | 2 cut(s) | 2137, 4381 |
| PaeR7I | CTCGAG | 2 cut(s) | 2918, 4877 |
| PagI | TCATGA | 1 cut(s) | 4830 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 4056 |
| PctI | GAATGC | 1 cut(s) | 1306 |
| PdmI | GAANNNNTTC | 5 cut(s) | 1346, 1758, 2346, 4613, 4670 |
| PflFI | GACNNNGTC | 1 cut(s) | 766 |
| PfoI | TCCNGGA | 1 cut(s) | 1783 |
| PkrI | GCNGC | 7 cut(s) | 2096, 2837, 2949, 2961, 3499, 3539, 4002 |
| Ple19I | CGATCG | 1 cut(s) | 4448 |
| PleI | GAGTC | 8 cut(s) | 612, 2173, 2917, 3053, 3104, 3871, 3958, 4790 |
| PmaCI | CACGTG | 1 cut(s) | 2992 |
| PmlI | CACGTG | 1 cut(s) | 2992 |
| PpsI | GAGTC | 8 cut(s) | 612, 2173, 2917, 3053, 3104, 3871, 3958, 4790 |
| Ppu21I | YACGTR | 2 cut(s) | 2992, 4856 |
| PpuMI | RGGWCCY | 1 cut(s) | 611 |
| PshAI | GACNNNNGTC | 2 cut(s) | 3074, 4563 |
| Psp124BI | GAGCTC | 3 cut(s) | 312, 1332, 2919 |
| Psp1406I | AACGTT | 1 cut(s) | 1574 |
| Psp5II | RGGWCCY | 1 cut(s) | 611 |
| Psp6I | CCWGG | 3 cut(s) | 1783, 3934, 4958 |
| PspCI | CACGTG | 1 cut(s) | 2992 |
| PspGI | CCWGG | 3 cut(s) | 1783, 3934, 4958 |
| PspN4I | GGNNCC | 8 cut(s) | 456, 613, 2027, 2199, 2443, 3450, 4322, 4456 |
| PspPI | GGNCC | 6 cut(s) | 218, 611, 742, 2442, 3163, 3667 |
| PspPPI | RGGWCCY | 1 cut(s) | 611 |
| PspXI | VCTCGAGB | 1 cut(s) | 4877 |
| PstI | CTGCAG | 1 cut(s) | 4648 |
| PstNI | CAGNNNCTG | 1 cut(s) | 2707 |
| PsuI | RGATCY | 8 cut(s) | 16, 2149, 2203, 2264, 3320, 3448, 3899, 5041 |
| PsyI | GACNNNGTC | 1 cut(s) | 766 |
| PvuI | CGATCG | 1 cut(s) | 4448 |
| PvuII | CAGCTG | 1 cut(s) | 4792 |
| RruI | TCGCGA | 1 cut(s) | 737 |
| RseI | CAYNNNNRTG | 6 cut(s) | 870, 2436, 2994, 3842, 4842, 5004 |
| SacI | GAGCTC | 3 cut(s) | 312, 1332, 2919 |
| SalI | GTCGAC | 2 cut(s) | 578, 2176 |
| SatI | GCNGC | 7 cut(s) | 2095, 2836, 2948, 2960, 3498, 3538, 4001 |
| Sau96I | GGNCC | 6 cut(s) | 218, 611, 742, 2442, 3163, 3667 |
| ScaI | AGTACT | 1 cut(s) | 4753 |
| SchI | GAGTC | 8 cut(s) | 613, 2173, 2917, 3053, 3104, 3872, 3959, 4790 |
| ScrFI | CCNGG | 4 cut(s) | 1785, 3936, 4337, 4960 |
| SduI | GDGCHC | 8 cut(s) | 312, 411, 1332, 2030, 2919, 4164, 4459, 5011 |
| SfcI | CTRYAG | 4 cut(s) | 2699, 3072, 3279, 4644 |
| Sfr274I | CTCGAG | 2 cut(s) | 2918, 4877 |
| SinI | GGWCC | 6 cut(s) | 218, 611, 742, 2442, 3163, 3667 |
| SlaI | CTCGAG | 2 cut(s) | 2918, 4877 |
| SmiMI | CAYNNNNRTG | 6 cut(s) | 870, 2436, 2994, 3842, 4842, 5004 |
| SmlI | CTYRAG | 8 cut(s) | 155, 197, 1949, 2848, 2918, 2932, 3860, 4877 |
| SmoI | CTYRAG | 8 cut(s) | 155, 197, 1949, 2848, 2918, 2932, 3860, 4877 |
| SnaBI | TACGTA | 1 cut(s) | 4856 |
| SpeI | ACTAGT | 1 cut(s) | 3841 |
| SphI | GCATGC | 2 cut(s) | 2137, 4381 |
| SsiI | CCGC | 7 cut(s) | 121, 150, 442, 719, 758, 3538, 4913 |
| SspI | AATATT | 2 cut(s) | 2355, 3909 |
| SstI | GAGCTC | 3 cut(s) | 312, 1332, 2919 |
| StyD4I | CCNGG | 4 cut(s) | 1783, 3934, 4335, 4958 |
| StyI | CCWWGG | 6 cut(s) | 1389, 3544, 3660, 3822, 4263, 4633 |
| TaiI | ACGT | 9 cut(s) | 579, 1577, 1795, 2558, 2790, 2994, 3524, 4167, 4858 |
| TatI | WGTACW | 9 cut(s) | 796, 1037, 1093, 1290, 1608, 1828, 3008, 3275, 4751 |
| TauI | GCSGC | 1 cut(s) | 3540 |
| TseFI | GTSAC | 3 cut(s) | 319, 3067, 3172 |
| TseI | GCWGC | 6 cut(s) | 2094, 2835, 2947, 2959, 3497, 4000 |
| Tsp45I | GTSAC | 3 cut(s) | 319, 3067, 3172 |
| TspGWI | ACGGA | 2 cut(s) | 2964, 3035 |
| Tth111I | GACNNNGTC | 1 cut(s) | 766 |
| Vha464I | CTTAAG | 1 cut(s) | 2848 |
| VpaK11BI | GGWCC | 6 cut(s) | 218, 611, 742, 2442, 3163, 3667 |
| XagI | CCTNNNNNAGG | 3 cut(s) | 1512, 2851, 3507 |
| XceI | RCATGY | 4 cut(s) | 1275, 2137, 3173, 4381 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 3658 |
| XhoI | CTCGAG | 2 cut(s) | 2918, 4877 |
| XmiI | GTMKAC | 3 cut(s) | 579, 2177, 5017 |
| XmnI | GAANNNNTTC | 5 cut(s) | 1346, 1758, 2346, 4613, 4670 |
| ZraI | GACGTC | 3 cut(s) | 577, 2556, 2788 |
| ZrmI | AGTACT | 1 cut(s) | 4753 |
| Zsp2I | ATGCAT | 2 cut(s) | 1306, 1728 |