Rroxscaffold_3G00240810

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000129
Physical Location & Seq
Reverse (-)
32620387 .. 32630369
9983 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00240810.1

Sequence Viewer

Length: 3324 bp
ATGACAACCAAAATAGATCTTCCTTCTTCTTCTTCTTCTTCTTATCCTCCTCTTTCTCCATTTCGTAAGTTAACATACGATGTGTTCCTCAGTTTCAGAGGTAAAGATACCCGCAACAATTTCACAGACCATCTTTATACCGCTCTCAAACAGAGAGGAATAAACACATTTAGAGATGATGAAGAACTTAAGAGTGGGGAGTCAATTGATCTAAATCTCTTGAGAGCAATTGAAGAGTCAAGATACGTTATTGTGGTTTTTTCTCAAAACTACGCCAATTCTGCGTGGTGTTTGAATGAGCTTGCAAAAGTCGCTGAATGCAGGAGAGTCATGGGACAAACAGTGCTCCCTGTGTTCTACCTTGTTGAGCCGTTTGAGGTACGAAGACAAAAAGGCGGTCACTTTGAGAAAGCATTTGAGAAGCATCAAGAGCGGTTTGGAGGCAATCCAGATAAATTGCAGAGGTGGAAAGACGCTCTTGCTGAAGTAGGCAATCTCTCTGGATGGCATCTGCAAGACGGGTACGAATCCATAGTTATTCAAGAAATTGTTGAAAAGATTTTCACCGAATTAAATCAAACAATCTCAATATCTGAGGGTTTAGTTGGGATGGATTCTCACCTGAATGAAATGCTTTCGTACTTAGACATTGGGTGTCCCGATGTTCGCATCGTAGGGATTTGTGGGATGGGCGGTATCGGTAAGACAACCATTGCACATGTGGTTTTTGAGACGGTAAAAGCTCGTTTTGAAGGTTGCAGCTTTCTTGAGAATGTGAGAGAAGTATCTGAAAAACATGGTGCAGTTCATTTACAGCAGCACCTTCTTTCGAATTTGCTGAAATGTAGTGTAAATGTAGAGAACACTAAAATGGGAAGAAGTATAATGAGGCAGAGACTACGTACTAAAATGGTTCTTATCATTCTTGATGATGTGGACCAAGAAGAACAATTGGAAGCGTTGTGTGATAGGGAATCGTTTGGTCCAGGGAGTAGAATCATCATAACTTGTAGAGATGAACATTTGCTCAGTGCAGTTGAAGGAGATGAAGTATATAAGGTGAATCAATTAACCGATGCTGAAGCTCTTCAGCTTTTTAGTATGAAAGCCTTCAAGAAAGGCCAGCTGGTTGGAGAAGAAATTCTCAAGCTATCGAAGGAATTTTTGAAATATGCTAATGGCCTTCCACTAGCTATTACAGTTTTGGGTTCTTCTGTTAAGGGCAGAAGAGTAGAGTTATGGTTAAGTGCATTGGATAGACTAAAAAACAATCCTCAAAAAAAAATTATCAATGTTCTTAAAGTTAGTTTTGATGGATTAGAAGAAACCGAAAAAAAAATGTTTTTGGACATCGCATGTTTCTTTAAAGGAGCGTATATAGATCATGTAACAAGAATACTACTAGGTAGTGACCACAGTCCGGACATTGATATAGAAGTTCTCATTGAGAAATCTTTGGTAACTCTGTTTGGAAGAAGATTAGGGATGCATGACTTGGTACAAGAATTGGGTTATGAAATTGTTCGTCAAGAATGCCGTGAAGAGCCTGGAAAACGTAGCAGGTTGTGGCTTTCTGATGAAATCATTCATGTACTTGGTCGGAGTAAGGCGACGAGTGCAGTTCAAAGCATATTCCTCCGATGTCGAACAGAAGACGATGTTGTCCACACAAGTGATGATGTCTTTTCGAATATGGACAGACTGAGATTGCTCAAGATCTGTAATTTAAAGTTCTCTGAAAACATCAGATATCTTTCTAATGAGTTACAGTATCTTGAATGGCATAAATGTCCTTTGGATTCTTTCCCATCAGACTTTCAACCGGATAAACTTACTGAAGTTCGCATGTACCGTAGCTGCATCAAACAACTATGGAGGGGGAAAAAAGGATGGAGCATGCTTCGGGTCATTAATCTGAGTGGTTCAAAATACTTGATGTCGACTCCGGACTTCACTGAGGTTCCTGATCTTGAAGAGTTGGTGCTTCAAAATTGCACAAGCTTGGTGGAGGTTCACCCGTCTCTCGGATTTCTGAAGAAACTTGGCTATTTGGATATGAAAAATTGCGAGTCTATTGAGAGCATTCCACCTTTCACCACCTTGGAATCCCTTCAAATATTGAGGCTTTCACTCTGTTCAGGACTGAAGAAGTTTCCAGAAATTGAAGGAAATATGAAAAGCTTGCTGGAGCTTTATTTGGATGGGACCAGCATTGAGGAATTGCCTCCATCAATTGAACGCTTTACTGATCTTACCGTGTTGAATCTAACAGACTGTAAAAACCTGTTGCATCTTCCAAATACCATTGGGTGCTTGACATCTCTGAAAAGTCTCCGTCTAACAGGTTGCTCCAAAATTGATGGGATACCTGAGAATCTGAATGATATGAAATGTCTGGAGATGCTTACAATTGGTGGAACTTCCATAAGGGAGTTATCTTTCATCGAAGGCATGAAGAATCTACAGTATCTATGCTTCCGAGGATGTAAATGTCTAGTTTTAGAATCATTTAAAGTTCTGGCTTCGTTATCGAATTTGACAGAGCTAGACTTGAGTTATTGCAATCTGATGGATGGAGCAATTCTCAATGATCTTAGCAGCCTAATCTCCTTAAGAATGTTAAATTTAAGTGGTAATTGCTTTGTATGGTTACCTGAAAGTATCTCTAAACTCTCAAAGCTCAACACTCTGACCTTGAGTAATTGCAGACAGCTTCAATTGCTGCCCAAGAAGCTTCCGTTAAGTCTTCGAAACGTGGATGCACAAGATTGTACTTCACTGACGGATTACCCAAATCAAATCAAAGTATTGAATTCATCGGAGTCGGGAGTGACTATTGTCAATTCCCTTAATTCTTCAGCACTTGAAGTCTTAGAATCCAGAGCGGTCATTTATCCAAGAAATGAAGGGGAATCCACAGAGCTGCTGTTGACACATCAGAATTCAGAAGGGAAACAAGTGGATCATTATTTGCCACTGAGAAGTGTAGCGCATCAAAGAGAAGAAGTGTCTTCCTGGTCAAATTTCTCTATCGTACTTCCTACAGATGAAATTCCAGAGTGGTTCAGCACTTCTACCAGAAATCCCATATCAATCTCGATGCCTCAAAATCTGACCGATGATAACAAGTGGAACGGATTTGCCGCGTGTGTTATATTCTCAGTCAAGGGGCATACTGCTCTCTCTATTATTGAACCGGATCCTGAATTTCCTAATTACTCATATCAAATGACATTGGAAACCGATGCTGGGCGTCTGAAACCATATGTGTTGGATAGGAAAGACCTTCTTCCCCCCTTGGATCAAGTTCTCATCTGCTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1107

Amino Acids

125.39

Weight (kDa)

5.63

Isoelectric Point (pI)

45.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 26 - 197 3.2e-50 TIR domain
TIR_2 PF13676 28 - 121 1.4e-16 TIR domain
NB-ARC PF00931 208 - 373 1.6e-23 NB-ARC domain
NACHT PF05729 224 - 368 2.2e-06 NACHT domain
WHD_ROQ1 PF23282 441 - 510 1.6e-16 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 669 - 783 3.3e-09 Leucine-rich repeat region
LRR_13 PF23286 701 - 786 5.7e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 739 - 847 8.2e-11 Leucine-rich repeat region
LRR_14 PF23598 840 - 938 2.5e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1661
Acc36I ACCTGC 1 cut(s) 1551
AccBSI CCGCTC 3 cut(s) 143, 433, 2885
AccI GTMKAC 1 cut(s) 1940
AccII CGCG 1 cut(s) 3146
AccIII TCCGGA 2 cut(s) 1420, 1945
AciI CCGC 7 cut(s) 112, 141, 396, 433, 693, 2885, 3144
AclWI GGATC 4 cut(s) 2970, 3194, 3207, 3309
AcuI CTGAAG 7 cut(s) 504, 1073, 1101, 1857, 2054, 2165, 2841
AcyI GRCGYC 1 cut(s) 3253
AfaI GTAC 9 cut(s) 381, 524, 641, 904, 1500, 1593, 1850, 2773, 3036
AfiI CCNNNNNNNGG 3 cut(s) 1420, 2024, 3249
AflII CTTAAG 2 cut(s) 188, 2611
AflIII ACRYGT 1 cut(s) 718
AjnI CCWGG 3 cut(s) 985, 1546, 3014
AleI CACNNNNGTG 1 cut(s) 1671
Alw21I GWGCWC 1 cut(s) 348
Alw26I GTCTC 4 cut(s) 725, 889, 2025, 2336
AlwI GGATC 4 cut(s) 2970, 3194, 3207, 3309
Aor13HI TCCGGA 2 cut(s) 1420, 1945
AoxI GGCC 2 cut(s) 1120, 1180
ApeKI GCWGC 6 cut(s) 759, 817, 1857, 2598, 2722, 2923
ArsI GACNNNNNNTTYG 2 cut(s) 382, 414
AseI ATTAAT 1 cut(s) 1911
Asp700I GAANNNNTTC 6 cut(s) 1086, 1109, 1140, 1521, 1584, 2109
AspLEI GCGC 1 cut(s) 2992
AspS9I GGNCC 3 cut(s) 937, 983, 2205
AsuHPI GGTGA 5 cut(s) 556, 611, 1072, 2006, 2086
AsuII TTCGAA 3 cut(s) 830, 1688, 2749
AvaII GGWCC 3 cut(s) 937, 983, 2205
BamHI GGATCC 1 cut(s) 3199
BarI GAAGNNNNNNTAC 2 cut(s) 2459, 2491
BbsI GAAGAC 4 cut(s) 391, 1659, 2738, 3003
Bbv12I GWGCWC 1 cut(s) 348
BbvI GCAGC 6 cut(s) 771, 829, 1844, 2610, 2709, 2910
BceAI ACGGC 2 cut(s) 355, 1521
BciT130I CCWGG 3 cut(s) 987, 1548, 3016
BciVI GTATCC 1 cut(s) 2358
BcoDI GTCTC 4 cut(s) 725, 889, 2025, 2336
BfaI CTAG 4 cut(s) 1190, 1403, 2495, 2546
BfmI CTRYAG 2 cut(s) 2462, 3042
BfrI CTTAAG 2 cut(s) 188, 2611
BfuAI ACCTGC 1 cut(s) 1551
BfuI GTATCC 1 cut(s) 2358
BglII AGATCT 2 cut(s) 16, 1716
BisI GCNGC 7 cut(s) 760, 818, 1858, 2599, 2723, 2924, 3144
BlsI GCNGC 7 cut(s) 761, 819, 1859, 2600, 2724, 2925, 3145
Bme1390I CCNGG 3 cut(s) 987, 1548, 3016
Bme18I GGWCC 3 cut(s) 937, 983, 2205
BmgT120I GGNCC 3 cut(s) 937, 983, 2205
BmiI GGNNCC 3 cut(s) 1962, 2206, 3201
BmrFI CCNGG 3 cut(s) 987, 1548, 3016
BoxI GACNNNNGTC 2 cut(s) 1416, 2837
BpiI GAAGAC 4 cut(s) 391, 1659, 2738, 3003
BplI GAGNNNNNCTC 2 cut(s) 2568, 2600
BpmI CTGGAG 2 cut(s) 2207, 2417
Bpu14I TTCGAA 3 cut(s) 830, 1688, 2749
BpuEI CTTGAG 6 cut(s) 241, 788, 1130, 1697, 2572, 2716
BsaAI YACGTR 1 cut(s) 902
BsaBI GATNNNNATC 1 cut(s) 213
BsaHI GRCGYC 1 cut(s) 3253
BsaJI CCNNGG 4 cut(s) 986, 2100, 2479, 3297
BsaWI WCCGGW 4 cut(s) 1420, 1822, 1945, 3196
BsaXI ACNNNNNCTCC 2 cut(s) 1035, 1065
Bsc4I CCNNNNNNNGG 3 cut(s) 1420, 2024, 3249
Bse3DI GCAATG 1 cut(s) 711
Bse8I GATNNNNATC 1 cut(s) 213
BseAI TCCGGA 2 cut(s) 1420, 1945
BseBI CCWGG 3 cut(s) 987, 1548, 3016
BseDI CCNNGG 4 cut(s) 986, 2100, 2479, 3297
BseGI GGATG 9 cut(s) 509, 615, 693, 1491, 1895, 2206, 2489, 2578, 2764
BseJI GATNNNNATC 1 cut(s) 213
BseLI CCNNNNNNNGG 3 cut(s) 1420, 2024, 3249
BseMI GCAATG 1 cut(s) 711
BseMII CTCAG 9 cut(s) 103, 585, 1042, 1694, 1907, 1947, 2361, 2969, 3174
BseRI GAGGAG 1 cut(s) 39
BseXI GCAGC 6 cut(s) 771, 829, 1844, 2610, 2709, 2910
BseYI CCCAGC 1 cut(s) 3248
BsgI GTGCAG 3 cut(s) 822, 1053, 1638
Bsh1236I CGCG 1 cut(s) 3146
BshFI GGCC 2 cut(s) 1122, 1182
BsiHKAI GWGCWC 1 cut(s) 348
BsiSI CCGG 4 cut(s) 1421, 1823, 1946, 3197
BslFI GGGAC 3 cut(s) 348, 642, 2218
BslI CCNNNNNNNGG 3 cut(s) 1420, 2024, 3249
BsmAI GTCTC 4 cut(s) 725, 889, 2025, 2336
BsmBI CGTCTC 2 cut(s) 725, 2025
BsmFI GGGAC 3 cut(s) 348, 642, 2218
BsmI GAATGC 3 cut(s) 323, 1538, 2082
BsnI GGCC 2 cut(s) 1122, 1182
Bsp119I TTCGAA 3 cut(s) 830, 1688, 2749
Bsp1286I GDGCHC 1 cut(s) 348
Bsp13I TCCGGA 2 cut(s) 1420, 1945
BspACI CCGC 7 cut(s) 112, 141, 396, 433, 693, 2885, 3144
BspANI GGCC 2 cut(s) 1122, 1182
BspCNI CTCAG 9 cut(s) 102, 586, 1041, 1695, 1908, 1948, 2362, 2970, 3173
BspEI TCCGGA 2 cut(s) 1420, 1945
BspFNI CGCG 1 cut(s) 3146
BspLI GGNNCC 3 cut(s) 1962, 2206, 3201
BspMI ACCTGC 1 cut(s) 1551
BspPI GGATC 4 cut(s) 2970, 3194, 3207, 3309
BspQI GCTCTTC 2 cut(s) 1092, 1536
BspT104I TTCGAA 3 cut(s) 830, 1688, 2749
BspTI CTTAAG 2 cut(s) 188, 2611
BsrBI CCGCTC 3 cut(s) 143, 433, 2885
BsrDI GCAATG 1 cut(s) 711
BssECI CCNNGG 4 cut(s) 986, 2100, 2479, 3297
BssNI GRCGYC 1 cut(s) 3253
BssT1I CCWWGG 2 cut(s) 2100, 3297
Bst2UI CCWGG 3 cut(s) 987, 1548, 3016
Bst4CI ACNGT 9 cut(s) 343, 736, 1201, 1418, 1770, 1853, 2257, 2276, 2466
Bst6I CTCTTC 5 cut(s) 228, 1092, 1222, 1536, 1968
BstACI GRCGYC 1 cut(s) 3253
BstAFI CTTAAG 2 cut(s) 188, 2611
BstBAI YACGTR 1 cut(s) 902
BstBI TTCGAA 3 cut(s) 830, 1688, 2749
BstC8I GCNNGC 4 cut(s) 303, 1124, 1898, 2183
BstEII GGTNACC 1 cut(s) 2649
BstF5I GGATG 9 cut(s) 509, 615, 693, 1491, 1895, 2206, 2489, 2578, 2764
BstFNI CGCG 1 cut(s) 3146
BstHHI GCGC 1 cut(s) 2992
BstMAI GTCTC 4 cut(s) 725, 889, 2025, 2336
BstMWI GCNNNNNNNGC 6 cut(s) 281, 311, 430, 1616, 2719, 2731
BstNI CCWGG 3 cut(s) 987, 1548, 3016
BstNSI RCATGY 4 cut(s) 722, 1359, 1849, 1900
BstPAI GACNNNNGTC 2 cut(s) 1416, 2837
BstPI GGTNACC 1 cut(s) 2649
BstSCI CCNGG 3 cut(s) 985, 1546, 3014
BstSFI CTRYAG 2 cut(s) 2462, 3042
BstSNI TACGTA 1 cut(s) 902
BstUI CGCG 1 cut(s) 3146
BstV1I GCAGC 6 cut(s) 771, 829, 1844, 2610, 2709, 2910
BstV2I GAAGAC 4 cut(s) 391, 1659, 2738, 3003
BstX2I RGATCY 3 cut(s) 16, 1716, 3199
BstXI CCANNNNNNTGG 1 cut(s) 1130
BstYI RGATCY 3 cut(s) 16, 1716, 3199
BsuI GTATCC 1 cut(s) 2358
BsuRI GGCC 2 cut(s) 1122, 1182
BtgZI GCGATG 1 cut(s) 1336
BtsCI GGATG 9 cut(s) 509, 615, 693, 1491, 1895, 2206, 2489, 2578, 2764
BtsIMutI CAGTG 5 cut(s) 348, 1036, 1953, 2777, 2975
BveI ACCTGC 1 cut(s) 1551
Cac8I GCNNGC 4 cut(s) 303, 1124, 1898, 2183
CfoI GCGC 1 cut(s) 2992
Cfr13I GGNCC 3 cut(s) 937, 983, 2205
CseI GACGC 2 cut(s) 482, 3242
Csp6I GTAC 9 cut(s) 380, 523, 640, 903, 1499, 1592, 1849, 2772, 3035
CspCI CAANNNNNGTGG 2 cut(s) 1986, 2021
CviQI GTAC 9 cut(s) 380, 523, 640, 903, 1499, 1592, 1849, 2772, 3035
DraI TTTAAA 3 cut(s) 1366, 1728, 2512
DrdI GACNNNNNNGTC 1 cut(s) 1661
DseDI GACNNNNNNGTC 1 cut(s) 1661
Eam1104I CTCTTC 5 cut(s) 228, 1092, 1222, 1536, 1968
EarI CTCTTC 5 cut(s) 228, 1092, 1222, 1536, 1968
Eco105I TACGTA 1 cut(s) 902
Eco130I CCWWGG 2 cut(s) 2100, 3297
Eco32I GATATC 1 cut(s) 1751
Eco47I GGWCC 3 cut(s) 937, 983, 2205
Eco57I CTGAAG 7 cut(s) 504, 1073, 1101, 1857, 2054, 2165, 2841
Eco91I GGTNACC 1 cut(s) 2649
EcoO65I GGTNACC 1 cut(s) 2649
EcoRI GAATTC 2 cut(s) 2812, 2941
EcoRII CCWGG 3 cut(s) 985, 1546, 3014
EcoRV GATATC 1 cut(s) 1751
EcoT14I CCWWGG 2 cut(s) 2100, 3297
EcoT22I ATGCAT 1 cut(s) 1491
ErhI CCWWGG 2 cut(s) 2100, 3297
Esp3I CGTCTC 2 cut(s) 725, 2025
FalI AAGNNNNNCTT 4 cut(s) 462, 494, 3273, 3305
FaqI GGGAC 3 cut(s) 348, 642, 2218
FauI CCCGC 1 cut(s) 119
FauNDI CATATG 1 cut(s) 3265
FblI GTMKAC 1 cut(s) 1940
Fnu4HI GCNGC 7 cut(s) 760, 818, 1858, 2599, 2723, 2924, 3144
FokI GGATG 9 cut(s) 516, 622, 700, 1498, 1902, 2213, 2496, 2585, 2771
Fsp4HI GCNGC 7 cut(s) 760, 818, 1858, 2599, 2723, 2924, 3144
FspBI CTAG 4 cut(s) 1190, 1403, 2495, 2546
GlaI GCGC 1 cut(s) 2991
GluI GCNGC 7 cut(s) 760, 818, 1858, 2599, 2723, 2924, 3144
GsaI CCCAGC 1 cut(s) 3252
GsuI CTGGAG 2 cut(s) 2207, 2417
HaeIII GGCC 2 cut(s) 1122, 1182
HapII CCGG 4 cut(s) 1421, 1823, 1946, 3197
HgaI GACGC 2 cut(s) 482, 3242
HhaI GCGC 1 cut(s) 2992
Hin1I GRCGYC 1 cut(s) 3253
Hin6I GCGC 1 cut(s) 2990
HinP1I GCGC 1 cut(s) 2990
HincII GTYRAC 3 cut(s) 72, 1941, 2931
HindII GTYRAC 3 cut(s) 72, 1941, 2931
HindIII AAGCTT 3 cut(s) 1999, 2179, 2732
HpaI GTTAAC 1 cut(s) 72
HpaII CCGG 4 cut(s) 1421, 1823, 1946, 3197
HphI GGTGA 5 cut(s) 556, 611, 1072, 2006, 2086
Hpy166II GTNNAC 6 cut(s) 72, 937, 1666, 1941, 2014, 2931
Hpy8I GTNNAC 6 cut(s) 72, 937, 1666, 1941, 2014, 2931
Hpy99I CGWCG 1 cut(s) 1615
HpyCH4III ACNGT 9 cut(s) 343, 736, 1201, 1418, 1770, 1853, 2257, 2276, 2466
HpyCH4IV ACGT 4 cut(s) 246, 901, 1555, 2754
HpyF10VI GCNNNNNNNGC 6 cut(s) 281, 311, 430, 1616, 2719, 2731
HpySE526I ACGT 4 cut(s) 246, 901, 1555, 2754
Hsp92I GRCGYC 1 cut(s) 3253
HspAI GCGC 1 cut(s) 2990
Kpn2I TCCGGA 2 cut(s) 1420, 1945
KspAI GTTAAC 1 cut(s) 72
LguI GCTCTTC 2 cut(s) 1092, 1536
LmnI GCTCC 6 cut(s) 351, 1370, 1893, 2188, 2354, 2576
Lsp1109I GCAGC 6 cut(s) 771, 829, 1844, 2610, 2709, 2910
MaeI CTAG 4 cut(s) 1190, 1403, 2495, 2546
MaeII ACGT 4 cut(s) 246, 901, 1555, 2754
MaeIII GTNAC 7 cut(s) 398, 1387, 1409, 1459, 1764, 2649, 2830
MbiI CCGCTC 3 cut(s) 143, 433, 2885
MfeI CAATTG 6 cut(s) 204, 228, 950, 2232, 2409, 2717
MflI RGATCY 3 cut(s) 16, 1716, 3199
MhlI GDGCHC 1 cut(s) 348
MlyI GAGTC 6 cut(s) 209, 245, 336, 1936, 2078, 2831
MmeI TCCRAC 3 cut(s) 1111, 1580, 3252
Mph1103I ATGCAT 1 cut(s) 1491
MroI TCCGGA 2 cut(s) 1420, 1945
MroXI GAANNNNTTC 6 cut(s) 1086, 1109, 1140, 1521, 1584, 2109
MslI CAYNNNNRTG 3 cut(s) 624, 869, 1671
MspA1I CMGCKG 1 cut(s) 1126
MspCI CTTAAG 2 cut(s) 188, 2611
MspI CCGG 4 cut(s) 1421, 1823, 1946, 3197
MspR9I CCNGG 3 cut(s) 987, 1548, 3016
MunI CAATTG 6 cut(s) 204, 228, 950, 2232, 2409, 2717
Mva1269I GAATGC 3 cut(s) 323, 1538, 2082
MvaI CCWGG 3 cut(s) 987, 1548, 3016
MvnI CGCG 1 cut(s) 3146
MwoI GCNNNNNNNGC 6 cut(s) 281, 311, 430, 1616, 2719, 2731
NdeI CATATG 1 cut(s) 3265
NlaIV GGNNCC 3 cut(s) 1962, 2206, 3201
NmuCI GTSAC 3 cut(s) 398, 1409, 2830
NsiI ATGCAT 1 cut(s) 1491
NspI RCATGY 4 cut(s) 722, 1359, 1849, 1900
NspV TTCGAA 3 cut(s) 830, 1688, 2749
OliI CACNNNNGTG 1 cut(s) 1671
PaeI GCATGC 1 cut(s) 1900
PciI ACATGT 1 cut(s) 718
PciSI GCTCTTC 2 cut(s) 1092, 1536
PcsI WCGNNNNNNNCGW 1 cut(s) 1849
PctI GAATGC 3 cut(s) 323, 1538, 2082
PdmI GAANNNNTTC 6 cut(s) 1086, 1109, 1140, 1521, 1584, 2109
PkrI GCNGC 7 cut(s) 761, 819, 1859, 2600, 2724, 2925, 3145
PleI GAGTC 6 cut(s) 208, 244, 335, 1936, 2077, 2830
PpsI GAGTC 6 cut(s) 208, 244, 335, 1936, 2077, 2830
Ppu21I YACGTR 1 cut(s) 902
PscI ACATGT 1 cut(s) 718
PshAI GACNNNNGTC 2 cut(s) 1416, 2837
PshBI ATTAAT 1 cut(s) 1911
Psp6I CCWGG 3 cut(s) 985, 1546, 3014
PspEI GGTNACC 1 cut(s) 2649
PspFI CCCAGC 1 cut(s) 3248
PspGI CCWGG 3 cut(s) 985, 1546, 3014
PspN4I GGNNCC 3 cut(s) 1962, 2206, 3201
PspPI GGNCC 3 cut(s) 937, 983, 2205
PsuI RGATCY 3 cut(s) 16, 1716, 3199
PvuII CAGCTG 1 cut(s) 1126
RsaI GTAC 9 cut(s) 381, 524, 641, 904, 1500, 1593, 1850, 2773, 3036
RsaNI GTAC 9 cut(s) 380, 523, 640, 903, 1499, 1592, 1849, 2772, 3035
RseI CAYNNNNRTG 3 cut(s) 624, 869, 1671
SalI GTCGAC 1 cut(s) 1939
SapI GCTCTTC 2 cut(s) 1092, 1536
SatI GCNGC 7 cut(s) 760, 818, 1858, 2599, 2723, 2924, 3144
Sau96I GGNCC 3 cut(s) 937, 983, 2205
SchI GAGTC 6 cut(s) 209, 245, 336, 1936, 2078, 2831
ScrFI CCNGG 3 cut(s) 987, 1548, 3016
SduI GDGCHC 1 cut(s) 348
SfcI CTRYAG 2 cut(s) 2462, 3042
SfuI TTCGAA 3 cut(s) 830, 1688, 2749
SinI GGWCC 3 cut(s) 937, 983, 2205
SmiMI CAYNNNNRTG 3 cut(s) 624, 869, 1671
SmlI CTYRAG 8 cut(s) 188, 220, 767, 1145, 1712, 2551, 2611, 2695
SmoI CTYRAG 8 cut(s) 188, 220, 767, 1145, 1712, 2551, 2611, 2695
SnaBI TACGTA 1 cut(s) 902
SphI GCATGC 1 cut(s) 1900
SsiI CCGC 7 cut(s) 112, 141, 396, 433, 693, 2885, 3144
SspI AATATT 1 cut(s) 2118
SspMI CTAG 4 cut(s) 1190, 1403, 2495, 2546
StyD4I CCNGG 3 cut(s) 985, 1546, 3014
StyI CCWWGG 2 cut(s) 2100, 3297
TaaI ACNGT 9 cut(s) 343, 736, 1201, 1418, 1770, 1853, 2257, 2276, 2466
TaiI ACGT 4 cut(s) 249, 904, 1558, 2757
TaqI TCGA 9 cut(s) 830, 1154, 1645, 1688, 1940, 2445, 2531, 2749, 3098
TaqII GACCGA 1 cut(s) 3131
TatI WGTACW 2 cut(s) 1591, 2771
TauI GCSGC 1 cut(s) 3146
TscAI CASTG 5 cut(s) 348, 1036, 1960, 2784, 2982
TseFI GTSAC 3 cut(s) 398, 1409, 2830
TseI GCWGC 6 cut(s) 759, 817, 1857, 2598, 2722, 2923
Tsp45I GTSAC 3 cut(s) 398, 1409, 2830
TspGWI ACGGA 4 cut(s) 2324, 2727, 2798, 3150
TspRI CASTG 5 cut(s) 348, 1036, 1960, 2784, 2982
Vha464I CTTAAG 2 cut(s) 188, 2611
VpaK11BI GGWCC 3 cut(s) 937, 983, 2205
VspI ATTAAT 1 cut(s) 1911
XceI RCATGY 4 cut(s) 722, 1359, 1849, 1900
XcmI CCANNNNNNNNNTGG 1 cut(s) 718
XmiI GTMKAC 1 cut(s) 1940
XmnI GAANNNNTTC 6 cut(s) 1086, 1109, 1140, 1521, 1584, 2109
XspI CTAG 4 cut(s) 1190, 1403, 2495, 2546
Zsp2I ATGCAT 1 cut(s) 1491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.