RLG00000000639

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000129
Physical Location & Seq
Reverse (-)
2942053 .. 2948943
6891 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000639

Sequence Viewer

Length: 1287 bp
ATGAAACAAATAGTCCTCCCAGTGTTCTATGATGTCGATCCATATGAGATGGTAATAGTTTTGTCCGATTACCTGAAATTGAATCAATCAACTGTGGTGGGGGCTAGGTTTGAAACCAACCACCCATTCATGGACGTCCAGGAATGTGGAATTCGTCTAGTCTATGAGCAAGATGATGGATGGTATACCAAACTGATCCCTGGTGATACTGGGACTTGTCTAAATGAAGGAGAAATTTATTCTACCATTGGAACAAGACTCCAGAATTCTGCTGAGAAAGAATATCAAGCGTTAACACATAATTCTGCAACGTATAGATTTAACCTCAGTGGAAGCCCAGCATGGTTTGACTACTCATGTGGTCTTAACACTGATACAGGGCCATGGCCCTCTGTGTCAATCGAGATTCCTCAGAATCTACACAAGAGTAAGAAGTGGATGGGATTTGCAATATGTGCATCAATTGCAGAGGAACAGCAGCATATGACACAAGAGGACTATTTTGTTTACTTTCAATTCATAATGGGTGAATCTAATGAGGATATTCCCCGAGAACTCGAGTATGTCGTGGACATGTGGTCAGAAGAACATAATCGACTTTTGGTTGTTTACATACCGCGAGCAGAGTTGCCCGAAGATTTTATGCATCCATCACCAACTGTAATGAACATTTTCTTCGGAACCAATTGCCCTTATGTAGAGGTTCGAAGAAGTGGTATCCGTATTGTGTACCAGGAAGATTTCCAAGGCTTTGTTGAGACAATCATCCAGTGCATGCAAAGAGAAGATACCCTTCAGCTTTATGATAAATGGGTGGTAGAAGATTGGATAGACTTGATAGGATTGCAAGGTGGTCATTTATATACTTCAAACAAACTTGATTCCATAACCCGACGAGCAAGAAAGTTCGAATTACTCTCTCAAAAATATAGAAATCAGGACTGGACTGCTCCATCACTGTATTTTTGTGTATTCTATGGAACTGAAATTTCCAAGTGGAAATGGTTCATGCATTTCAACGAAGGCAACTCAGCAGAAATCCAACTGCCCCCGAATTTGTTTGATGATGCTAATTGGTTAGGCATTGCTGTATGTGCTTTTTTGTGGGTATCCCACGAAGATCCAAACATGATTCTTGGCACTCCTGATTCAGAGTTTAATGTGTTCTGGAGGCAATGCAATCTGGCCAGGATCTTGTACAGACACTTACACTCTGTGAATTACCTTGAAGTGAGTACCCATATTTGCATTGACAAGAAGCGGAGTCTCCAAGCAAGGGCTTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

429

Amino Acids

50.0

Weight (kDa)

4.93

Isoelectric Point (pI)

45.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C-JID PF20160 113 - 247 2.7e-06 C-JID domain
C-JID PF20160 334 - 385 5.2e-06 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 138
AccI GTMKAC 1 cut(s) 185
AccII CGCG 1 cut(s) 619
AciI CCGC 2 cut(s) 617, 1261
AclWI GGATC 4 cut(s) 32, 190, 1115, 1199
AcoI YGGCCR 1 cut(s) 1185
AcsI RAATTY 5 cut(s) 150, 234, 265, 987, 1054
AcuI CTGAAG 1 cut(s) 779
AcyI GRCGYC 1 cut(s) 135
AdeI CACNNNGTG 1 cut(s) 1216
AfaI GTAC 3 cut(s) 731, 1199, 1237
AfiI CCNNNNNNNGG 2 cut(s) 130, 1276
AflIII ACRYGT 1 cut(s) 573
AgsI TTSAA 6 cut(s) 82, 113, 515, 870, 1018, 1229
AhdI GACNNNNNGTC 1 cut(s) 577
AjnI CCWGG 4 cut(s) 138, 199, 732, 1187
AjuI GAANNNNNNNTTGG 2 cut(s) 110, 142
AluBI AGCT 1 cut(s) 799
AluI AGCT 1 cut(s) 799
Alw26I GTCTC 2 cut(s) 752, 1271
AlwI GGATC 4 cut(s) 32, 190, 1115, 1199
Ama87I CYCGRG 2 cut(s) 549, 557
AoxI GGCC 3 cut(s) 380, 386, 1185
ApeKI GCWGC 1 cut(s) 478
ApoI RAATTY 5 cut(s) 150, 234, 265, 987, 1054
Asp700I GAANNNNTTC 2 cut(s) 671, 1004
AspS9I GGNCC 2 cut(s) 380, 387
AsuHPI GGTGA 3 cut(s) 215, 539, 645
AsuII TTCGAA 2 cut(s) 706, 909
AvaI CYCGRG 2 cut(s) 549, 557
BalI TGGCCA 1 cut(s) 1187
BbvI GCAGC 1 cut(s) 490
BccI CCATC 6 cut(s) 43, 170, 174, 433, 658, 961
BciT130I CCWGG 4 cut(s) 140, 201, 734, 1189
BciVI GTATCC 2 cut(s) 728, 1120
BcoDI GTCTC 2 cut(s) 752, 1271
BfaI CTAG 2 cut(s) 105, 158
BfuI GTATCC 2 cut(s) 728, 1120
BisI GCNGC 1 cut(s) 479
BlsI GCNGC 1 cut(s) 480
Bme1390I CCNGG 4 cut(s) 140, 201, 734, 1189
BmeRI GACNNNNNGTC 1 cut(s) 577
BmeT110I CYCGRG 2 cut(s) 549, 557
BmgT120I GGNCC 2 cut(s) 380, 387
BmiI GGNNCC 1 cut(s) 682
BmrFI CCNGG 4 cut(s) 140, 201, 734, 1189
BmrI ACTGGG 2 cut(s) 14, 219
BmsI GCATC 3 cut(s) 467, 655, 1057
BmuI ACTGGG 2 cut(s) 14, 219
BpmI CTGGAG 2 cut(s) 245, 1189
Bpu14I TTCGAA 2 cut(s) 706, 909
BsaBI GATNNNNATC 1 cut(s) 36
BsaHI GRCGYC 1 cut(s) 135
BsaJI CCNNGG 3 cut(s) 199, 383, 745
Bsc4I CCNNNNNNNGG 2 cut(s) 130, 1276
Bse1I ACTGG 4 cut(s) 20, 214, 769, 947
Bse3DI GCAATG 2 cut(s) 1083, 1181
Bse8I GATNNNNATC 1 cut(s) 36
BseBI CCWGG 4 cut(s) 140, 201, 734, 1189
BseDI CCNNGG 3 cut(s) 199, 383, 745
BseGI GGATG 4 cut(s) 185, 444, 646, 765
BseJI GATNNNNATC 1 cut(s) 36
BseLI CCNNNNNNNGG 2 cut(s) 130, 1276
BseMI GCAATG 2 cut(s) 1083, 1181
BseMII CTCAG 4 cut(s) 264, 340, 425, 1044
BseNI ACTGG 4 cut(s) 20, 214, 769, 947
BseXI GCAGC 1 cut(s) 490
BseYI CCCAGC 1 cut(s) 337
Bsh1236I CGCG 1 cut(s) 619
BshFI GGCC 3 cut(s) 382, 388, 1187
BsiHKCI CYCGRG 2 cut(s) 549, 557
BslFI GGGAC 1 cut(s) 226
BslI CCNNNNNNNGG 2 cut(s) 130, 1276
BsmAI GTCTC 2 cut(s) 752, 1271
BsmFI GGGAC 1 cut(s) 226
BsnI GGCC 3 cut(s) 382, 388, 1187
BsoBI CYCGRG 2 cut(s) 549, 557
Bsp119I TTCGAA 2 cut(s) 706, 909
Bsp1407I TGTACA 1 cut(s) 1197
Bsp143I GATC 4 cut(s) 37, 195, 1120, 1191
Bsp19I CCATGG 1 cut(s) 383
BspACI CCGC 2 cut(s) 617, 1261
BspANI GGCC 3 cut(s) 382, 388, 1187
BspCNI CTCAG 4 cut(s) 265, 339, 424, 1043
BspFNI CGCG 1 cut(s) 619
BspLI GGNNCC 1 cut(s) 682
BspPI GGATC 4 cut(s) 32, 190, 1115, 1199
BspT104I TTCGAA 2 cut(s) 706, 909
BsrDI GCAATG 2 cut(s) 1083, 1181
BsrGI TGTACA 1 cut(s) 1197
BsrI ACTGG 4 cut(s) 20, 214, 769, 947
BssECI CCNNGG 3 cut(s) 199, 383, 745
BssMI GATC 4 cut(s) 37, 195, 1120, 1191
BssNAI GTATAC 1 cut(s) 186
BssNI GRCGYC 1 cut(s) 135
BssT1I CCWWGG 2 cut(s) 383, 745
Bst1107I GTATAC 1 cut(s) 186
Bst2UI CCWGG 4 cut(s) 140, 201, 734, 1189
Bst4CI ACNGT 3 cut(s) 94, 661, 960
BstACI GRCGYC 1 cut(s) 135
BstAPI GCANNNNNTGC 2 cut(s) 455, 464
BstAUI TGTACA 1 cut(s) 1197
BstBI TTCGAA 2 cut(s) 706, 909
BstC8I GCNNGC 2 cut(s) 621, 776
BstDEI CTNAG 4 cut(s) 273, 326, 411, 1030
BstDSI CCRYGG 1 cut(s) 383
BstF5I GGATG 4 cut(s) 185, 444, 646, 765
BstFNI CGCG 1 cut(s) 619
BstKTI GATC 4 cut(s) 40, 198, 1123, 1194
BstMAI GTCTC 2 cut(s) 752, 1271
BstMBI GATC 4 cut(s) 37, 195, 1120, 1191
BstMWI GCNNNNNNNGC 3 cut(s) 455, 464, 1094
BstNI CCWGG 4 cut(s) 140, 201, 734, 1189
BstNSI RCATGY 2 cut(s) 577, 778
BstSCI CCNGG 4 cut(s) 138, 199, 732, 1187
BstUI CGCG 1 cut(s) 619
BstV1I GCAGC 1 cut(s) 490
BstX2I RGATCY 2 cut(s) 1120, 1191
BstXI CCANNNNNNTGG 1 cut(s) 146
BstYI RGATCY 2 cut(s) 1120, 1191
BstZ17I GTATAC 1 cut(s) 186
BsuI GTATCC 2 cut(s) 728, 1120
BsuRI GGCC 3 cut(s) 382, 388, 1187
BtgI CCRYGG 1 cut(s) 383
BtsCI GGATG 4 cut(s) 185, 444, 646, 765
BtsIMutI CAGTG 5 cut(s) 27, 334, 369, 776, 956
Cac8I GCNNGC 2 cut(s) 621, 776
Cfr13I GGNCC 2 cut(s) 380, 387
Csp6I GTAC 3 cut(s) 730, 1198, 1236
CspCI CAANNNNNGTGG 2 cut(s) 78, 113
CviAII CATG 8 cut(s) 130, 342, 357, 384, 574, 775, 1009, 1129
CviJI RGCY 8 cut(s) 104, 336, 382, 388, 750, 799, 1187, 1280
CviKI_1 RGCY 8 cut(s) 104, 336, 382, 388, 750, 799, 1187, 1280
CviQI GTAC 3 cut(s) 730, 1198, 1236
DdeI CTNAG 4 cut(s) 273, 326, 411, 1030
DpnI GATC 4 cut(s) 39, 197, 1122, 1193
DpnII GATC 4 cut(s) 37, 195, 1120, 1191
DraIII CACNNNGTG 1 cut(s) 1216
DriI GACNNNNNGTC 1 cut(s) 577
EaeI YGGCCR 1 cut(s) 1185
Eam1105I GACNNNNNGTC 1 cut(s) 577
Eco130I CCWWGG 2 cut(s) 383, 745
Eco57I CTGAAG 1 cut(s) 779
Eco88I CYCGRG 2 cut(s) 549, 557
EcoRI GAATTC 2 cut(s) 150, 265
EcoRII CCWGG 4 cut(s) 138, 199, 732, 1187
EcoT14I CCWWGG 2 cut(s) 383, 745
EcoT22I ATGCAT 2 cut(s) 648, 1014
ErhI CCWWGG 2 cut(s) 383, 745
FaeI CATG 8 cut(s) 133, 345, 360, 387, 577, 778, 1012, 1132
FalI AAGNNNNNCTT 2 cut(s) 777, 809
FaqI GGGAC 1 cut(s) 226
FatI CATG 8 cut(s) 129, 341, 356, 383, 573, 774, 1008, 1128
FauNDI CATATG 2 cut(s) 43, 483
FblI GTMKAC 1 cut(s) 185
Fnu4HI GCNGC 1 cut(s) 479
FokI GGATG 4 cut(s) 192, 451, 633, 752
Fsp4HI GCNGC 1 cut(s) 479
FspBI CTAG 2 cut(s) 105, 158
GluI GCNGC 1 cut(s) 479
GsaI CCCAGC 1 cut(s) 341
GsuI CTGGAG 2 cut(s) 245, 1189
HaeIII GGCC 3 cut(s) 382, 388, 1187
Hin1I GRCGYC 1 cut(s) 135
Hin1II CATG 8 cut(s) 133, 345, 360, 387, 577, 778, 1012, 1132
HincII GTYRAC 1 cut(s) 294
HindII GTYRAC 1 cut(s) 294
HinfI GANTC 9 cut(s) 82, 258, 406, 415, 530, 881, 1132, 1148, 1264
HpaI GTTAAC 1 cut(s) 294
HphI GGTGA 3 cut(s) 215, 539, 645
Hpy166II GTNNAC 6 cut(s) 186, 294, 508, 571, 610, 730
Hpy188I TCNGA 5 cut(s) 67, 414, 583, 680, 1153
Hpy188III TCNNGA 5 cut(s) 262, 403, 938, 1145, 1168
Hpy8I GTNNAC 6 cut(s) 186, 294, 508, 571, 610, 730
Hpy99I CGWCG 1 cut(s) 897
HpyAV CCTTC 3 cut(s) 221, 803, 1016
HpyCH4III ACNGT 3 cut(s) 94, 661, 960
HpyCH4IV ACGT 2 cut(s) 135, 311
HpyF10VI GCNNNNNNNGC 3 cut(s) 455, 464, 1094
HpyF3I CTNAG 4 cut(s) 273, 326, 411, 1030
HpySE526I ACGT 2 cut(s) 135, 311
Hsp92I GRCGYC 1 cut(s) 135
Hsp92II CATG 8 cut(s) 133, 345, 360, 387, 577, 778, 1012, 1132
KspAI GTTAAC 1 cut(s) 294
Kzo9I GATC 4 cut(s) 37, 195, 1120, 1191
LmnI GCTCC 1 cut(s) 955
Lsp1109I GCAGC 1 cut(s) 490
LweI GCATC 3 cut(s) 467, 655, 1057
MaeI CTAG 2 cut(s) 105, 158
MaeII ACGT 2 cut(s) 135, 311
MalI GATC 4 cut(s) 39, 197, 1122, 1193
MboI GATC 4 cut(s) 37, 195, 1120, 1191
MboII GAAGA 8 cut(s) 596, 647, 667, 720, 749, 797, 833, 1130
MfeI CAATTG 2 cut(s) 462, 685
MflI RGATCY 2 cut(s) 1120, 1191
MlsI TGGCCA 1 cut(s) 1187
MluNI TGGCCA 1 cut(s) 1187
MlyI GAGTC 2 cut(s) 252, 1273
MmeI TCCRAC 1 cut(s) 1066
MnlI CCTC 9 cut(s) 26, 335, 400, 420, 463, 487, 532, 694, 1164
Mox20I TGGCCA 1 cut(s) 1187
Mph1103I ATGCAT 2 cut(s) 648, 1014
MroXI GAANNNNTTC 2 cut(s) 671, 1004
MscI TGGCCA 1 cut(s) 1187
MseI TTAA 4 cut(s) 293, 321, 366, 1158
Msp20I TGGCCA 1 cut(s) 1187
MspR9I CCNGG 4 cut(s) 140, 201, 734, 1189
MunI CAATTG 2 cut(s) 462, 685
MvaI CCWGG 4 cut(s) 140, 201, 734, 1189
MvnI CGCG 1 cut(s) 619
MwoI GCNNNNNNNGC 3 cut(s) 455, 464, 1094
NcoI CCATGG 1 cut(s) 383
NdeI CATATG 2 cut(s) 43, 483
NdeII GATC 4 cut(s) 37, 195, 1120, 1191
NlaIII CATG 8 cut(s) 133, 345, 360, 387, 577, 778, 1012, 1132
NlaIV GGNNCC 1 cut(s) 682
NsiI ATGCAT 2 cut(s) 648, 1014
NspI RCATGY 2 cut(s) 577, 778
NspV TTCGAA 2 cut(s) 706, 909
PaeI GCATGC 1 cut(s) 778
PaeR7I CTCGAG 1 cut(s) 557
PciI ACATGT 1 cut(s) 573
PcsI WCGNNNNNNNCGW 1 cut(s) 564
PdmI GAANNNNTTC 2 cut(s) 671, 1004
PfeI GAWTC 7 cut(s) 82, 406, 415, 530, 881, 1132, 1148
PfoI TCCNGGA 1 cut(s) 138
PkrI GCNGC 1 cut(s) 480
PleI GAGTC 2 cut(s) 252, 1272
PpsI GAGTC 2 cut(s) 252, 1272
PscI ACATGT 1 cut(s) 573
Psp6I CCWGG 4 cut(s) 138, 199, 732, 1187
PspFI CCCAGC 1 cut(s) 337
PspGI CCWGG 4 cut(s) 138, 199, 732, 1187
PspN4I GGNNCC 1 cut(s) 682
PspPI GGNCC 2 cut(s) 380, 387
PspXI VCTCGAGB 1 cut(s) 557
PsuI RGATCY 2 cut(s) 1120, 1191
RsaI GTAC 3 cut(s) 731, 1199, 1237
RsaNI GTAC 3 cut(s) 730, 1198, 1236
SaqAI TTAA 4 cut(s) 293, 321, 366, 1158
SatI GCNGC 1 cut(s) 479
Sau3AI GATC 4 cut(s) 37, 195, 1120, 1191
Sau96I GGNCC 2 cut(s) 380, 387
SchI GAGTC 2 cut(s) 252, 1273
ScrFI CCNGG 4 cut(s) 140, 201, 734, 1189
SetI ASST 9 cut(s) 75, 110, 138, 314, 327, 705, 801, 853, 1227
SfaNI GCATC 3 cut(s) 467, 655, 1057
Sfr274I CTCGAG 1 cut(s) 557
SfuI TTCGAA 2 cut(s) 706, 909
SlaI CTCGAG 1 cut(s) 557
SmlI CTYRAG 1 cut(s) 557
SmoI CTYRAG 1 cut(s) 557
SphI GCATGC 1 cut(s) 778
SsiI CCGC 2 cut(s) 617, 1261
SspMI CTAG 2 cut(s) 105, 158
StyD4I CCNGG 4 cut(s) 138, 199, 732, 1187
StyI CCWWGG 2 cut(s) 383, 745
TaaI ACNGT 3 cut(s) 94, 661, 960
TaiI ACGT 2 cut(s) 138, 314
TaqI TCGA 6 cut(s) 36, 402, 558, 595, 706, 909
TatI WGTACW 1 cut(s) 1197
TfiI GAWTC 7 cut(s) 82, 406, 415, 530, 881, 1132, 1148
Tru1I TTAA 4 cut(s) 293, 321, 366, 1158
Tru9I TTAA 4 cut(s) 293, 321, 366, 1158
TscAI CASTG 5 cut(s) 27, 334, 376, 776, 963
TseI GCWGC 1 cut(s) 478
TspDTI ATGAA 6 cut(s) 17, 118, 240, 508, 680, 997
TspGWI ACGGA 1 cut(s) 710
TspRI CASTG 5 cut(s) 27, 334, 376, 776, 963
XapI RAATTY 5 cut(s) 150, 234, 265, 987, 1054
XceI RCATGY 2 cut(s) 577, 778
XhoI CTCGAG 1 cut(s) 557
XmiI GTMKAC 1 cut(s) 185
XmnI GAANNNNTTC 2 cut(s) 671, 1004
XspI CTAG 2 cut(s) 105, 158
ZraI GACGTC 1 cut(s) 136
Zsp2I ATGCAT 2 cut(s) 648, 1014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.