RLG00000000664

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000129
Physical Location & Seq
Reverse (-)
3308178 .. 3316356
8179 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000664

Sequence Viewer

Length: 2997 bp
ATGTTTAACACCAATACAGATATAATTCCTTCTTCTTCTTCTTCTTCTTATTCTTCTCCACCAACCGGTAAATTTACGTACGATGTGTTCCTCAGTTTCAGAGGTAAAGATACCGGCAAAAATTTCACGGATCATCTTTATACTGCTCTCAAACAGAAAGGTATATCCACTTTTAGAGATGATGAAGAACTCGAGAGGGGGGAATCGATTGGTCCAAATCTCTTGAAAGCAATCGAAGAATCAAGATATGTGGTTGTTGTTTTCTCACGAAACTATGCCGATTCTGCATGGTGCTTGGATGAGATTGCCAAGGTCGCCGATTGCATGAAAGCCATGGGAACAAAAGTCCCTCCAGTGTTCTACGACGTTGATCCGAATGAGGTACGAAGACAAACAGAGGGTCACTTTGGGAAAGCAGTTGAGAAGCATCAAAAGCGGTATAAAGTGGAACCAGACAAAGTAAAGAGGTGGAAAGACGCTCTTTTTCATGTAGGCAATCTCTCTGGATGGCATCTGCAAGATAGTTGCAGCTTTCTTGAGAATATTAGAGAGGAAGCAGAAAAGCAAGGTGCAATTCATTTACAAAAGCAACTTCTTTTGAACTTTCTGAAATGTAATGTAAATGTACAGACCACTAAAATGGGAAAAGATATAATCAGAAGTAGACTATGTACTAAAATGGTTCTTCTCATTCTTGATGATGTGGATCAAGAAGAACAATTGGAAGTATTGTGCAATTGCACTTGGTTTGGTCCAGGGAGTAGGATCATATTAACATCAAGAGATGAACACTTGCTCAGTGCATTTGGAGTGGATAAAGTATACAAGGTGAATCCATTAACTGCTGCCGAAGCTCTTGAGCTCTTTGGTATGAAAGCCTTTAAGAAAGACCAGCTGGTTGGGGATGATTTTATCGAGCTATCCAAGGAATTTTTGAAATATGCTGACGGCCTTCCATTAGCTATTAAAGTTTTGGGTTCCTCTGTTCATGGTAAAAATGTAAAATTGTGGTCTAGTGCATTGGACAGACTAAAATCCAATCCTCAAAAAAAAATTATCAATGTTTTTAAAGTTAGTTTTGATGGATTAGAAGAAACCGAAAAGAAAATATCTTTGGACATCGCATGTTTCTTTAAAGGATGGTATATAGATCAGGTAACAAGAATACTACAAGGTACTGACCACTGTCCGGACATTGATATAGAAGTTCTGGTAAATAAATCTTTGGTAACTCTATTTGGAAGAAAATTGTTGATGCATGACTTGATACAAGAATTGGGTTGGGAAATTGTTCGTCAAGAATGCCGTGAAGAGCCTGGAAAGCGTAGTAGGTTGTGGCTTCCAAATGAAATCATCCGTGTACTTGATCGGAGTAAGGCGACGAGTGCAATTCAAAGCATATACCTCCAATGTCCAACAGGAGACGATGGATGTAGCATGCTACGGCTTATTAATCTGTGGGGTTCACAATACTTGATGTCAACTCCAGACTTCACTGAGGTTCCTGAACTTAAAGTGTTAGTGCTTCGAAATTTCTCAAGCTTGGTGGAGGTTCACCCGTCTCTTGGATTTCTGAAGAAACTTCGCTATTTGGATATGACAAATTGCAAGTCCATTGAGAGCCTTCCACCTTTCACTAGCTTGGAATCCCTTGAAAAATTATGGCTTTCACTCTGTTCAGAACTGAAGAAGTTTCCAGAAATTGAAGGAAATATGAAAAGCTTGCTGGAGCTTCATTTGGATGGGACCAGCATTGAGGAATTGCCTCCATCAATTGAACGCTTGACTGGTCTTACCATGTTGAATCTAGCAGACTGTCAAAACCTTTTGCGTCTTCCCAATACCATTGGGTGCTTGACATCTTTGAAAAGTCTCGATCTAACAGGTTGCTCCAAAATCGATGCGATACCTGAGAATCTGAATGGACTCGGAGCCTCACAAAGTTACCGAATGTCGGTGATTCCAAAAGAGGAGGGAGGATCTCCAGAGCTGCGTGTGACGTATTCAGGGGGCAAAGAAATGCCTTACTGCTCAGAGTTCAGTGGCATATTTCCTAGGGATGAAATTCCAGAGTGGTTCAGTACTATATCTACCAGAAATCCCATATCAATGTCAATACCGCAAAAGCTGGCCGATGACAACAAGTGGAAGGGATTTGCCGCGTGTGCTATATTCTCAGTCAAGGGGCATACTGCTCTCTCCGTTGTTGAACCGGATCCTGATTTTCTTAATTACTCGTATCAAATCACATTGGAAACTGATGTTGTGCATCGAGAACCATTTGTGTTCGACTGTCATGAAGGACGCCGCCCCCTTGGATCAAGTTCTCATCTGCTTATGATTTTCTATATATCTCGTTCAGAGGTTCCACAGTGGGGGTTAAATCTATCAACTGTGGTGAGGGCTATGTTTGAAACCCCCAATCCATCCATGGTGGTCCAGAAATGTGGAATTCGTCTAGTCTATGAGCAAGATGCTGGATGGCATTTCAGAATAGCCACCGGCGGTAGTCCGATTTGTCTGAATGAAGTTGATTACGACGCCTCTGTATTGGAGTCTGGCTGGCTTAGTCAATCTTTGGAATGGACTGGTCTGACCCTCGTTCTGTTTCTTGTCACTTCAACGGAGCTAAAATTTTCAAGTGGAAAAGTTGTCCCCGACCCATATCCAAACATTAGTCTTGGCTGTACTGCTCCTGATTCAGAAGATGTTATGTTTACTTGCCATCTGGACTCTGATGTTGCTTGGCAGGGTTTATTTGATTCCGACACGCAAATAAATTTTAAGAGTCTAGAGCCATCGGTATTCGATATGACTTGGTTCTTTTATACACCGCCCACTAGGCTTAGTAAGGTTTGGAAGCAATGTAAATTGGCCAAGATAACTATGTGGTTCTCTAGCCCATGCTTAGCGGTGCATAGTTGTGCCCTTCGTCTACTATTTAAGGAGGACGTTGAACATCTTGTAGAAACACTGACCCTCACCGAATTACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

999

Amino Acids

112.73

Weight (kDa)

5.53

Isoelectric Point (pI)

47.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 27 - 185 3.6e-48 TIR domain
TIR_2 PF13676 29 - 125 3.1e-16 TIR domain
NB-ARC PF00931 192 - 297 3.6e-11 NB-ARC domain
WHD_ROQ1 PF23282 364 - 433 9.9e-16 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 511 - 632 4.2e-12 Leucine-rich repeat region
LRR_13 PF23286 548 - 634 6.4e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 684 - 826 5.5e-09 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 664, 822, 2935
AccII CGCG 1 cut(s) 2162
AccIII TCCGGA 1 cut(s) 1189
AciI CCGC 7 cut(s) 436, 2120, 2160, 2308, 2505, 2834, 2912
AclWI GGATC 8 cut(s) 138, 365, 714, 773, 1987, 2210, 2223, 2326
AcoI YGGCCR 2 cut(s) 2130, 2874
AcsI RAATTY 8 cut(s) 71, 121, 929, 1531, 2064, 2451, 2633, 2779
AcuI CTGAAG 2 cut(s) 1595, 1706
AcyI GRCGYC 2 cut(s) 2305, 2541
AfaI GTAC 8 cut(s) 80, 384, 627, 673, 1177, 1362, 2083, 2689
AfiI CCNNNNNNNGG 5 cut(s) 65, 1189, 1565, 1954, 2375
AgeI ACCGGT 1 cut(s) 65
AjnI CCWGG 2 cut(s) 754, 1315
AjuI GAANNNNNNNTTGG 2 cut(s) 1097, 1129
Alw21I GWGCWC 1 cut(s) 864
Alw26I GTCTC 3 cut(s) 1416, 1566, 1877
AlwI GGATC 8 cut(s) 138, 365, 714, 773, 1987, 2210, 2223, 2326
Ama87I CYCGRG 1 cut(s) 191
Aor13HI TCCGGA 1 cut(s) 1189
AoxI GGCC 3 cut(s) 949, 2130, 2874
ApeKI GCWGC 3 cut(s) 528, 845, 1990
ApoI RAATTY 8 cut(s) 71, 121, 929, 1531, 2064, 2451, 2633, 2779
ArsI GACNNNNNNTTYG 4 cut(s) 385, 417, 1595, 1627
AseI ATTAAT 1 cut(s) 1452
AsiGI ACCGGT 1 cut(s) 65
Asp700I GAANNNNTTC 1 cut(s) 1290
AspA2I CCTAGG 1 cut(s) 2054
AspS9I GGNCC 4 cut(s) 212, 752, 1746, 2437
AsuHPI GGTGA 5 cut(s) 841, 1547, 1969, 2410, 2974
AsuII TTCGAA 1 cut(s) 1528
AvaI CYCGRG 1 cut(s) 191
AvaII GGWCC 4 cut(s) 212, 752, 1746, 2437
AvrII CCTAGG 1 cut(s) 2054
BaeGI GKGCMC 1 cut(s) 2929
BalI TGGCCA 1 cut(s) 2876
BamHI GGATCC 1 cut(s) 2215
BanII GRGCYC 1 cut(s) 864
BbsI GAAGAC 2 cut(s) 394, 1826
Bbv12I GWGCWC 1 cut(s) 864
BbvI GCAGC 3 cut(s) 540, 832, 1977
BceAI ACGGC 3 cut(s) 964, 1290, 1460
BciT130I CCWGG 2 cut(s) 756, 1317
BcoDI GTCTC 3 cut(s) 1416, 1566, 1877
BfaI CTAG 8 cut(s) 1014, 1638, 1808, 2055, 2459, 2792, 2841, 2898
BglI GCCNNNNNGGC 1 cut(s) 2842
BisI GCNGC 5 cut(s) 529, 846, 1991, 2160, 2308
BlnI CCTAGG 1 cut(s) 2054
BlpI GCTNAGC 1 cut(s) 2908
BlsI GCNGC 5 cut(s) 530, 847, 1992, 2161, 2309
BmcAI AGTACT 1 cut(s) 2083
Bme1390I CCNGG 2 cut(s) 756, 1317
Bme18I GGWCC 4 cut(s) 212, 752, 1746, 2437
BmeT110I CYCGRG 1 cut(s) 191
BmgT120I GGNCC 4 cut(s) 212, 752, 1746, 2437
BmiI GGNNCC 7 cut(s) 450, 979, 1503, 1747, 1933, 2217, 2367
BmrFI CCNGG 2 cut(s) 756, 1317
BmsI GCATC 6 cut(s) 436, 520, 1245, 1891, 2278, 2464
BoxI GACNNNNGTC 1 cut(s) 1185
BpiI GAAGAC 2 cut(s) 394, 1826
BpmI CTGGAG 4 cut(s) 336, 1470, 1748, 1968
Bpu1102I GCTNAGC 1 cut(s) 2908
Bpu14I TTCGAA 1 cut(s) 1528
BpuEI CTTGAG 3 cut(s) 557, 878, 1522
Bsa29I ATCGAT 2 cut(s) 206, 1899
BsaAI YACGTR 1 cut(s) 78
BsaBI GATNNNNATC 1 cut(s) 705
BsaHI GRCGYC 2 cut(s) 2305, 2541
BsaJI CCNNGG 7 cut(s) 309, 333, 755, 924, 2054, 2314, 2430
BsaWI WCCGGW 3 cut(s) 65, 1189, 2212
Bsc4I CCNNNNNNNGG 5 cut(s) 65, 1189, 1565, 1954, 2375
Bse118I RCCGGY 3 cut(s) 65, 113, 2501
Bse1I ACTGG 3 cut(s) 353, 1792, 2593
Bse3DI GCAATG 1 cut(s) 2870
Bse8I GATNNNNATC 1 cut(s) 705
BseAI TCCGGA 1 cut(s) 1189
BseBI CCWGG 2 cut(s) 756, 1317
BseCI ATCGAT 2 cut(s) 206, 1899
BseDI CCNNGG 7 cut(s) 309, 333, 755, 924, 2054, 2314, 2430
BseJI GATNNNNATC 1 cut(s) 705
BseLI CCNNNNNNNGG 5 cut(s) 65, 1189, 1565, 1954, 2375
BseMI GCAATG 1 cut(s) 2870
BseMII CTCAG 6 cut(s) 106, 811, 1488, 1902, 2046, 2190
BseNI ACTGG 3 cut(s) 353, 1792, 2593
BseRI GAGGAG 1 cut(s) 1985
BseSI GKGCMC 1 cut(s) 2929
BseXI GCAGC 3 cut(s) 540, 832, 1977
Bsh1236I CGCG 1 cut(s) 2162
BshFI GGCC 3 cut(s) 951, 2132, 2876
BshTI ACCGGT 1 cut(s) 65
BshVI ATCGAT 2 cut(s) 206, 1899
BsiHKAI GWGCWC 1 cut(s) 864
BsiHKCI CYCGRG 1 cut(s) 191
BsiSI CCGG 5 cut(s) 66, 114, 1190, 2213, 2502
BsiWI CGTACG 1 cut(s) 78
BslFI GGGAC 3 cut(s) 332, 1759, 2639
BslI CCNNNNNNNGG 5 cut(s) 65, 1189, 1565, 1954, 2375
BsmAI GTCTC 3 cut(s) 1416, 1566, 1877
BsmBI CGTCTC 2 cut(s) 1416, 1566
BsmFI GGGAC 3 cut(s) 332, 1759, 2639
BsmI GAATGC 1 cut(s) 1307
BsnI GGCC 3 cut(s) 951, 2132, 2876
BsoBI CYCGRG 1 cut(s) 191
Bsp119I TTCGAA 1 cut(s) 1528
Bsp1286I GDGCHC 2 cut(s) 864, 2929
Bsp13I TCCGGA 1 cut(s) 1189
Bsp1407I TGTACA 1 cut(s) 625
Bsp1720I GCTNAGC 1 cut(s) 2908
Bsp19I CCATGG 2 cut(s) 333, 2430
BspACI CCGC 7 cut(s) 436, 2120, 2160, 2308, 2505, 2834, 2912
BspANI GGCC 3 cut(s) 951, 2132, 2876
BspCNI CTCAG 6 cut(s) 105, 810, 1489, 1903, 2045, 2189
BspDI ATCGAT 2 cut(s) 206, 1899
BspEI TCCGGA 1 cut(s) 1189
BspFNI CGCG 1 cut(s) 2162
BspHI TCATGA 1 cut(s) 2296
BspLI GGNNCC 7 cut(s) 450, 979, 1503, 1747, 1933, 2217, 2367
BspPI GGATC 8 cut(s) 138, 365, 714, 773, 1987, 2210, 2223, 2326
BspQI GCTCTTC 1 cut(s) 1305
BspT104I TTCGAA 1 cut(s) 1528
BsrDI GCAATG 1 cut(s) 2870
BsrFI RCCGGY 3 cut(s) 65, 113, 2501
BsrGI TGTACA 1 cut(s) 625
BsrI ACTGG 3 cut(s) 353, 1792, 2593
BssAI RCCGGY 3 cut(s) 65, 113, 2501
BssECI CCNNGG 7 cut(s) 309, 333, 755, 924, 2054, 2314, 2430
BssNAI GTATAC 1 cut(s) 823
BssNI GRCGYC 2 cut(s) 2305, 2541
BssT1I CCWWGG 6 cut(s) 309, 333, 924, 2054, 2314, 2430
Bst1107I GTATAC 1 cut(s) 823
Bst2UI CCWGG 2 cut(s) 756, 1317
Bst4CI ACNGT 5 cut(s) 1187, 1817, 2294, 2373, 2395
Bst6I CTCTTC 1 cut(s) 1305
BstACI GRCGYC 2 cut(s) 2305, 2541
BstAUI TGTACA 1 cut(s) 625
BstBAI YACGTR 1 cut(s) 78
BstBI TTCGAA 1 cut(s) 1528
BstC8I GCNNGC 4 cut(s) 1439, 1724, 2130, 2564
BstDEI CTNAG 9 cut(s) 92, 797, 1497, 1911, 2032, 2176, 2567, 2846, 2908
BstDSI CCRYGG 2 cut(s) 333, 2430
BstFNI CGCG 1 cut(s) 2162
BstMAI GTCTC 3 cut(s) 1416, 1566, 1877
BstMWI GCNNNNNNNGC 8 cut(s) 284, 314, 433, 851, 1321, 1385, 2165, 2842
BstNI CCWGG 2 cut(s) 756, 1317
BstNSI RCATGY 2 cut(s) 1128, 1441
BstPAI GACNNNNGTC 1 cut(s) 1185
BstSCI CCNGG 2 cut(s) 754, 1315
BstSLI GKGCMC 1 cut(s) 2929
BstSNI TACGTA 1 cut(s) 78
BstUI CGCG 1 cut(s) 2162
BstV1I GCAGC 3 cut(s) 540, 832, 1977
BstV2I GAAGAC 2 cut(s) 394, 1826
BstX2I RGATCY 2 cut(s) 1979, 2215
BstXI CCANNNNNNTGG 3 cut(s) 640, 899, 2447
BstYI RGATCY 2 cut(s) 1979, 2215
BstZ17I GTATAC 1 cut(s) 823
Bsu15I ATCGAT 2 cut(s) 206, 1899
BsuRI GGCC 3 cut(s) 951, 2132, 2876
BsuTUI ATCGAT 2 cut(s) 206, 1899
BtgI CCRYGG 2 cut(s) 333, 2430
BtgZI GCGATG 1 cut(s) 1105
BtsIMutI CAGTG 7 cut(s) 360, 805, 1183, 1494, 2047, 2378, 2972
Cac8I GCNNGC 4 cut(s) 1439, 1724, 2130, 2564
CciI TCATGA 1 cut(s) 2296
Cfr10I RCCGGY 3 cut(s) 65, 113, 2501
Cfr13I GGNCC 4 cut(s) 212, 752, 1746, 2437
ClaI ATCGAT 2 cut(s) 206, 1899
CseI GACGC 4 cut(s) 485, 1820, 2313, 2549
Csp6I GTAC 8 cut(s) 79, 383, 626, 672, 1176, 1361, 2082, 2688
CspAI ACCGGT 1 cut(s) 65
CspCI CAANNNNNGTGG 4 cut(s) 231, 266, 1527, 1562
CviQI GTAC 8 cut(s) 79, 383, 626, 672, 1176, 1361, 2082, 2688
DdeI CTNAG 9 cut(s) 92, 797, 1497, 1911, 2032, 2176, 2567, 2846, 2908
DraI TTTAAA 2 cut(s) 1069, 1135
EaeI YGGCCR 2 cut(s) 2130, 2874
Eam1104I CTCTTC 1 cut(s) 1305
EarI CTCTTC 1 cut(s) 1305
Ecl136II GAGCTC 1 cut(s) 862
Eco105I TACGTA 1 cut(s) 78
Eco130I CCWWGG 6 cut(s) 309, 333, 924, 2054, 2314, 2430
Eco24I GRGCYC 1 cut(s) 864
Eco47I GGWCC 4 cut(s) 212, 752, 1746, 2437
Eco53kI GAGCTC 1 cut(s) 862
Eco57I CTGAAG 2 cut(s) 1595, 1706
Eco88I CYCGRG 1 cut(s) 191
EcoICRI GAGCTC 1 cut(s) 862
EcoRI GAATTC 1 cut(s) 2451
EcoRII CCWGG 2 cut(s) 754, 1315
EcoT14I CCWWGG 6 cut(s) 309, 333, 924, 2054, 2314, 2430
EcoT22I ATGCAT 1 cut(s) 1260
EcoT38I GRGCYC 1 cut(s) 864
ErhI CCWWGG 6 cut(s) 309, 333, 924, 2054, 2314, 2430
Esp3I CGTCTC 2 cut(s) 1416, 1566
FalI AAGNNNNNCTT 2 cut(s) 465, 497
FaqI GGGAC 3 cut(s) 332, 1759, 2639
FblI GTMKAC 3 cut(s) 664, 822, 2935
Fnu4HI GCNGC 5 cut(s) 529, 846, 1991, 2160, 2308
FriOI GRGCYC 1 cut(s) 864
Fsp4HI GCNGC 5 cut(s) 529, 846, 1991, 2160, 2308
FspBI CTAG 8 cut(s) 1014, 1638, 1808, 2055, 2459, 2792, 2841, 2898
GluI GCNGC 5 cut(s) 529, 846, 1991, 2160, 2308
GsuI CTGGAG 4 cut(s) 336, 1470, 1748, 1968
HaeIII GGCC 3 cut(s) 951, 2132, 2876
HapII CCGG 5 cut(s) 66, 114, 1190, 2213, 2502
HgaI GACGC 4 cut(s) 485, 1820, 2313, 2549
Hin1I GRCGYC 2 cut(s) 2305, 2541
HincII GTYRAC 1 cut(s) 1482
HindII GTYRAC 1 cut(s) 1482
HindIII AAGCTT 2 cut(s) 1540, 1720
HpaII CCGG 5 cut(s) 66, 114, 1190, 2213, 2502
HphI GGTGA 5 cut(s) 841, 1547, 1969, 2410, 2974
Hpy166II GTNNAC 8 cut(s) 665, 823, 1361, 1466, 1482, 1555, 2718, 2936
Hpy8I GTNNAC 8 cut(s) 665, 823, 1361, 1466, 1482, 1555, 2718, 2936
Hpy99I CGWCG 3 cut(s) 368, 1384, 2543
HpyAV CCTTC 7 cut(s) 39, 962, 1634, 1700, 2143, 2294, 2939
HpyCH4III ACNGT 5 cut(s) 1187, 1817, 2294, 2373, 2395
HpyCH4IV ACGT 4 cut(s) 77, 366, 2000, 2952
HpyF10VI GCNNNNNNNGC 8 cut(s) 284, 314, 433, 851, 1321, 1385, 2165, 2842
HpyF3I CTNAG 9 cut(s) 92, 797, 1497, 1911, 2032, 2176, 2567, 2846, 2908
HpySE526I ACGT 4 cut(s) 77, 366, 2000, 2952
Hsp92I GRCGYC 2 cut(s) 2305, 2541
Kpn2I TCCGGA 1 cut(s) 1189
LguI GCTCTTC 1 cut(s) 1305
LmnI GCTCC 5 cut(s) 1729, 1895, 1931, 2626, 2698
Lsp1109I GCAGC 3 cut(s) 540, 832, 1977
LweI GCATC 6 cut(s) 436, 520, 1245, 1891, 2278, 2464
MaeI CTAG 8 cut(s) 1014, 1638, 1808, 2055, 2459, 2792, 2841, 2898
MaeII ACGT 4 cut(s) 77, 366, 2000, 2952
MaeIII GTNAC 6 cut(s) 401, 1156, 1228, 1943, 1996, 2614
MfeI CAATTG 3 cut(s) 719, 736, 1773
MflI RGATCY 2 cut(s) 1979, 2215
MhlI GDGCHC 2 cut(s) 864, 2929
MlsI TGGCCA 1 cut(s) 2876
MluNI TGGCCA 1 cut(s) 2876
MlyI GAGTC 4 cut(s) 1920, 2564, 2726, 2797
MmeI TCCRAC 2 cut(s) 1439, 2790
Mox20I TGGCCA 1 cut(s) 2876
Mph1103I ATGCAT 1 cut(s) 1260
MroI TCCGGA 1 cut(s) 1189
MroXI GAANNNNTTC 1 cut(s) 1290
MscI TGGCCA 1 cut(s) 2876
MslI CAYNNNNRTG 4 cut(s) 638, 1740, 2108, 2922
Msp20I TGGCCA 1 cut(s) 2876
MspA1I CMGCKG 1 cut(s) 895
MspI CCGG 5 cut(s) 66, 114, 1190, 2213, 2502
MspR9I CCNGG 2 cut(s) 756, 1317
MunI CAATTG 3 cut(s) 719, 736, 1773
Mva1269I GAATGC 1 cut(s) 1307
MvaI CCWGG 2 cut(s) 756, 1317
MvnI CGCG 1 cut(s) 2162
MwoI GCNNNNNNNGC 8 cut(s) 284, 314, 433, 851, 1321, 1385, 2165, 2842
NcoI CCATGG 2 cut(s) 333, 2430
NlaIV GGNNCC 7 cut(s) 450, 979, 1503, 1747, 1933, 2217, 2367
NmuCI GTSAC 3 cut(s) 401, 1996, 2614
NsiI ATGCAT 1 cut(s) 1260
NspI RCATGY 2 cut(s) 1128, 1441
NspV TTCGAA 1 cut(s) 1528
PaeI GCATGC 1 cut(s) 1441
PaeR7I CTCGAG 1 cut(s) 191
PagI TCATGA 1 cut(s) 2296
PciSI GCTCTTC 1 cut(s) 1305
PctI GAATGC 1 cut(s) 1307
PdmI GAANNNNTTC 1 cut(s) 1290
Pfl23II CGTACG 1 cut(s) 78
PinAI ACCGGT 1 cut(s) 65
PkrI GCNGC 5 cut(s) 530, 847, 1992, 2161, 2309
PleI GAGTC 4 cut(s) 1920, 2563, 2726, 2796
PpsI GAGTC 4 cut(s) 1920, 2563, 2726, 2796
Ppu21I YACGTR 1 cut(s) 78
PshAI GACNNNNGTC 1 cut(s) 1185
PshBI ATTAAT 1 cut(s) 1452
Psp124BI GAGCTC 1 cut(s) 864
Psp6I CCWGG 2 cut(s) 754, 1315
PspGI CCWGG 2 cut(s) 754, 1315
PspLI CGTACG 1 cut(s) 78
PspN4I GGNNCC 7 cut(s) 450, 979, 1503, 1747, 1933, 2217, 2367
PspPI GGNCC 4 cut(s) 212, 752, 1746, 2437
PsrI GAACNNNNNNTAC 2 cut(s) 71, 103
PsuI RGATCY 2 cut(s) 1979, 2215
PvuII CAGCTG 1 cut(s) 895
RsaI GTAC 8 cut(s) 80, 384, 627, 673, 1177, 1362, 2083, 2689
RsaNI GTAC 8 cut(s) 79, 383, 626, 672, 1176, 1361, 2082, 2688
RseI CAYNNNNRTG 4 cut(s) 638, 1740, 2108, 2922
SacI GAGCTC 1 cut(s) 864
SapI GCTCTTC 1 cut(s) 1305
SatI GCNGC 5 cut(s) 529, 846, 1991, 2160, 2308
Sau96I GGNCC 4 cut(s) 212, 752, 1746, 2437
ScaI AGTACT 1 cut(s) 2083
SchI GAGTC 4 cut(s) 1920, 2564, 2726, 2797
ScrFI CCNGG 2 cut(s) 756, 1317
SduI GDGCHC 2 cut(s) 864, 2929
SfaNI GCATC 6 cut(s) 436, 520, 1245, 1891, 2278, 2464
Sfr274I CTCGAG 1 cut(s) 191
SfuI TTCGAA 1 cut(s) 1528
SgrAI CRCCGGYG 1 cut(s) 2501
SinI GGWCC 4 cut(s) 212, 752, 1746, 2437
SlaI CTCGAG 1 cut(s) 191
SmiMI CAYNNNNRTG 4 cut(s) 638, 1740, 2108, 2922
SmlI CTYRAG 4 cut(s) 191, 536, 857, 1537
SmoI CTYRAG 4 cut(s) 191, 536, 857, 1537
SnaBI TACGTA 1 cut(s) 78
SphI GCATGC 1 cut(s) 1441
SsiI CCGC 7 cut(s) 436, 2120, 2160, 2308, 2505, 2834, 2912
SspI AATATT 1 cut(s) 544
SspMI CTAG 8 cut(s) 1014, 1638, 1808, 2055, 2459, 2792, 2841, 2898
SstI GAGCTC 1 cut(s) 864
StyD4I CCNGG 2 cut(s) 754, 1315
StyI CCWWGG 6 cut(s) 309, 333, 924, 2054, 2314, 2430
TaaI ACNGT 5 cut(s) 1187, 1817, 2294, 2373, 2395
TaiI ACGT 4 cut(s) 80, 369, 2003, 2955
TatI WGTACW 5 cut(s) 625, 671, 1360, 2081, 2687
TauI GCSGC 2 cut(s) 2162, 2310
TscAI CASTG 7 cut(s) 360, 805, 1190, 1501, 2047, 2378, 2979
TseFI GTSAC 3 cut(s) 401, 1996, 2614
TseI GCWGC 3 cut(s) 528, 845, 1990
Tsp45I GTSAC 3 cut(s) 401, 1996, 2614
TspGWI ACGGA 4 cut(s) 143, 1346, 2191, 2639
TspRI CASTG 7 cut(s) 360, 805, 1190, 1501, 2047, 2378, 2979
VpaK11BI GGWCC 4 cut(s) 212, 752, 1746, 2437
VspI ATTAAT 1 cut(s) 1452
XapI RAATTY 8 cut(s) 71, 121, 929, 1531, 2064, 2451, 2633, 2779
XbaI TCTAGA 1 cut(s) 2791
XceI RCATGY 2 cut(s) 1128, 1441
XcmI CCANNNNNNNNNTGG 1 cut(s) 2428
XhoI CTCGAG 1 cut(s) 191
XmaJI CCTAGG 1 cut(s) 2054
XmiI GTMKAC 3 cut(s) 664, 822, 2935
XmnI GAANNNNTTC 1 cut(s) 1290
XspI CTAG 8 cut(s) 1014, 1638, 1808, 2055, 2459, 2792, 2841, 2898
ZrmI AGTACT 1 cut(s) 2083
Zsp2I ATGCAT 1 cut(s) 1260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.