Rw7G041280

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000129
Physical Location & Seq
Forward (+)
64305555 .. 64308302
2748 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G041280.1

Sequence Viewer

Length: 2085 bp
ATGCTTTCGTACTTAGACATTGGGTGTCCTGATGTTCGAATCATAGGGATTTGTGGGATGGGTGGTATCGGTAAGACAACTGTTGCACAAGTGGTTTTTGAAAGGGTACAAGCTCAGTTTGAAGGTTGCAGCTTTCTTGAGAATGTTAGAGAGGAAACAGAAAAGCAAGGTGCAATTCATTTACAAGAGCAACTTCTTTTGAACTTGCTGAATTGTAATGTAAATGTACAGACCACTAAAATGGGAAAAGATATAATCAGGCATAGACTATGTACTAAAAGGGTTCTTCTCATTCTTGATGATGTGGATCAAGAAGAACAATTGGAAGCATTGTGTAATCGCACTTGGTTTGGTCCAGGGAGTAGGATCATCCTAACATCAAGAGATGAACAATTGCTCAGTTTATTTAGAGTGGATAAAGTATACAAGGTGAATCCATTAACTGCTTGCGAAGCTCTTGAGCTCTTTGGTATGAAAGCCTTTAAGAAAGACCAGCTGGTTGGGAAAGATTTTCTCAAGCTATCCAAGGAATTTTTGAAATATGCTAATGGTCTTCCATTAGCTATTAAAGTTTTGGGTTCCTCTGTTTATGGTAGGAATGTAAAATTATGGTCTAGTGCATTGGATAGACTAAAAAACAATCCTCAAAAAAAGATTATCAATGTTCTTAAAGCTAGTTATGATGGATTAGAAGAAACTGAAAAGAAAATATTTTTGGACATCGCATGTTTCTTTAAGGGATGGAACATAGCTCGTGTAACAAGTATACTACAAGGTAGTGGCCACTGCCCGGACATTGATATAGAGGTTCTCGTACAGAAATCTTTGGTAACGCTGTTTGGAAGCGAATTGGGGATGCATGACTTGGTACAAGAACTGGGTTGGGATATTGTTCGTCAAGAAAGTCCTGAAGAGCCCGAAAATCGTAGCAGGTTGTGGCTTGCCGATGAAATCATCTATGTTCTTGGTCGAAGTAAGGCGATGAGTGCTGCTCAAAGTATATTCCTCCAATGTCCAACAAAAGACGATTTTGTCTACTCAATTGATAATGCCTTCTCAAATATGGACAGATTGAGATTGCTCAAGATTTGTAATGTGAAGTTTTTCGGAAACATCACATATCTTTCTAATGAGTTACAATATCTTGAGTGGCATGAGTGTCCTTTGGATTCTTTCCCGTCAGAATTTCAAGCAAATAAACTTGTTGAAGTTCGCATGCAATTTAGCCGCGTCAAACAACTATGGAGGGGGAAAAAAGGATGGAGCAAGCTACAACTTATTGATCTGAGTGGTTCACCATACTTGATGTCGACTCCAGACTTCACTGAGGTTCCTGATCTTCATGAATTGGAGCTTCAATATTGTACAAGCTTGGTGGAGGTTCACCCGTCTCTTGGATTTCTGAAGAAACTTGTCTCTCTGAATATGACACATTGCAGGTCTATTGAGAGCCTTCCACCTTTCACTGCCCTTGAATCCCTCCAAATATTGAGGCTTTCATTCTGTTCAGGGCTGAAGAAGTTTCCAGAAATTGAAGGAAATATGAAAAGCTTGCTGGAGCTTCATTTGGATAGAACCAGCATTGAGGAATTGCCTTCATCAATTGAGCACTTGACTGGTCTTACCGTATTGAATCTAACAGACTGTAAAAACCTTTTGCGTCTTCCCGATACCGTTGGGTGCTTGACTTCTTTGAAAAGTCTCCATCTAACAGGTTGCTCCAAAATTGATGAGATACCTGAGAATCTGAAAGGTATGATATGTCTGGAGAAGCTTACAATTGGTGGAACTTCCATAAGGGAGTTATCTTTCATTGTAGGCATGAAGAATCTACGGTATCTATCCTGCGAAGGATCTTCAATTGCTGCCCAAGAAGCTTCCGTTAAGTCTTCATCACGTGTATGCACAAGATTGTACTTCACTGACAGATTACCCAAATCAAATCAAAGAATTGAATTCATTGGAGTCGGGACTGACTATTATCGATTCCCTCAATTCTTCAGCACTTGGAGTCTCACAAAGTTCGTCCTGGTTGAATTTTTCAAGCATTGTAGGGGAACCTCTACAAGCGCATGTGACATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

694

Amino Acids

78.64

Weight (kDa)

6.86

Isoelectric Point (pI)

43.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 11 - 164 3.7e-22 NB-ARC domain
WHD_ROQ1 PF23282 231 - 302 1.4e-18 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 473 - 617 8.4e-14 Leucine-rich repeat region
LRR_13 PF23286 491 - 578 1.4e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1031
Acc36I ACCTGC 2 cut(s) 921, 1428
AccI GTMKAC 4 cut(s) 423, 766, 1035, 1310
AccII CGCG 1 cut(s) 1230
AciI CCGC 1 cut(s) 1228
AclWI GGATC 3 cut(s) 315, 374, 1861
AcoI YGGCCR 1 cut(s) 781
AcsI RAATTY 4 cut(s) 530, 1184, 1955, 2036
AcuI CTGAAG 4 cut(s) 930, 1424, 1535, 1984
AcvI CACGTG 1 cut(s) 1898
AfaI GTAC 8 cut(s) 11, 108, 228, 274, 816, 870, 1366, 1916
AfiI CCNNNNNNNGG 2 cut(s) 790, 1394
AflIII ACRYGT 1 cut(s) 1897
AjnI CCWGG 2 cut(s) 355, 2028
AjuI GAANNNNNNNTTGG 2 cut(s) 698, 730
Alw21I GWGCWC 2 cut(s) 465, 1611
Alw26I GTCTC 4 cut(s) 1395, 1420, 1706, 2018
AlwI GGATC 3 cut(s) 315, 374, 1861
AoxI GGCC 1 cut(s) 781
ApeKI GCWGC 3 cut(s) 129, 989, 1865
ApoI RAATTY 4 cut(s) 530, 1184, 1955, 2036
Asp700I GAANNNNTTC 1 cut(s) 1103
AspLEI GCGC 1 cut(s) 2072
AspS9I GGNCC 1 cut(s) 353
AsuC2I CCSGG 1 cut(s) 791
AsuHPI GGTGA 3 cut(s) 442, 1287, 1376
AsuII TTCGAA 1 cut(s) 37
AvaII GGWCC 1 cut(s) 353
BalI TGGCCA 1 cut(s) 783
BanII GRGCYC 2 cut(s) 465, 918
BauI CACGAG 1 cut(s) 753
BbrPI CACGTG 1 cut(s) 1898
BbsI GAAGAC 3 cut(s) 545, 1655, 1881
Bbv12I GWGCWC 2 cut(s) 465, 1611
BbvI GCAGC 3 cut(s) 141, 976, 1852
BccI CCATC 5 cut(s) 52, 677, 735, 1254, 1713
BciT130I CCWGG 2 cut(s) 357, 2030
BcnI CCSGG 1 cut(s) 791
BcoDI GTCTC 4 cut(s) 1395, 1420, 1706, 2018
BfaI CTAG 2 cut(s) 615, 675
BfuAI ACCTGC 2 cut(s) 921, 1428
BisI GCNGC 4 cut(s) 130, 990, 1228, 1866
BlsI GCNGC 4 cut(s) 131, 991, 1229, 1867
Bme1390I CCNGG 3 cut(s) 357, 791, 2030
Bme18I GGWCC 1 cut(s) 353
BmgT120I GGNCC 1 cut(s) 353
BmiI GGNNCC 3 cut(s) 580, 1332, 2059
BmrFI CCNGG 3 cut(s) 357, 791, 2030
BmrI ACTGGG 1 cut(s) 887
BmsI GCATC 1 cut(s) 846
BmuI ACTGGG 1 cut(s) 887
BpiI GAAGAC 3 cut(s) 545, 1655, 1881
BplI GAGNNNNNCTC 2 cut(s) 976, 1008
BpmI CTGGAG 3 cut(s) 1299, 1577, 1787
Bpu14I TTCGAA 1 cut(s) 37
BpuEI CTTGAG 5 cut(s) 158, 479, 500, 1067, 1166
BpuMI CCSGG 1 cut(s) 791
Bsa29I ATCGAT 1 cut(s) 1984
BsaAI YACGTR 1 cut(s) 1898
BsaBI GATNNNNATC 1 cut(s) 306
BsaJI CCNNGG 2 cut(s) 356, 525
Bsc4I CCNNNNNNNGG 2 cut(s) 790, 1394
Bse1I ACTGG 2 cut(s) 882, 1621
Bse3DI GCAATG 1 cut(s) 1432
Bse8I GATNNNNATC 1 cut(s) 306
BseBI CCWGG 2 cut(s) 357, 2030
BseCI ATCGAT 1 cut(s) 1984
BseDI CCNNGG 2 cut(s) 356, 525
BseGI GGATG 5 cut(s) 63, 369, 746, 861, 1265
BseJI GATNNNNATC 1 cut(s) 306
BseLI CCNNNNNNNGG 2 cut(s) 790, 1394
BseMI GCAATG 1 cut(s) 1432
BseMII CTCAG 5 cut(s) 128, 412, 1277, 1317, 1731
BseNI ACTGG 2 cut(s) 882, 1621
BseXI GCAGC 3 cut(s) 141, 976, 1852
Bsh1236I CGCG 1 cut(s) 1230
BshFI GGCC 1 cut(s) 783
BshVI ATCGAT 1 cut(s) 1984
BsiHKAI GWGCWC 2 cut(s) 465, 1611
BsiSI CCGG 1 cut(s) 791
BslFI GGGAC 1 cut(s) 1984
BslI CCNNNNNNNGG 2 cut(s) 790, 1394
BsmAI GTCTC 4 cut(s) 1395, 1420, 1706, 2018
BsmBI CGTCTC 1 cut(s) 1395
BsmFI GGGAC 1 cut(s) 1984
BsnI GGCC 1 cut(s) 783
Bsp119I TTCGAA 1 cut(s) 37
Bsp1286I GDGCHC 3 cut(s) 465, 918, 1611
Bsp1407I TGTACA 2 cut(s) 226, 1364
Bsp143I GATC 5 cut(s) 307, 366, 1282, 1336, 1853
BspACI CCGC 1 cut(s) 1228
BspANI GGCC 1 cut(s) 783
BspCNI CTCAG 5 cut(s) 127, 411, 1278, 1318, 1732
BspDI ATCGAT 1 cut(s) 1984
BspFNI CGCG 1 cut(s) 1230
BspHI TCATGA 1 cut(s) 1342
BspLI GGNNCC 3 cut(s) 580, 1332, 2059
BspMI ACCTGC 2 cut(s) 921, 1428
BspPI GGATC 3 cut(s) 315, 374, 1861
BspQI GCTCTTC 1 cut(s) 906
BspT104I TTCGAA 1 cut(s) 37
BsrDI GCAATG 1 cut(s) 1432
BsrGI TGTACA 2 cut(s) 226, 1364
BsrI ACTGG 2 cut(s) 882, 1621
BssECI CCNNGG 2 cut(s) 356, 525
BssMI GATC 5 cut(s) 307, 366, 1282, 1336, 1853
BssNAI GTATAC 2 cut(s) 424, 767
BssSI CACGAG 1 cut(s) 753
BssT1I CCWWGG 1 cut(s) 525
Bst1107I GTATAC 2 cut(s) 424, 767
Bst2BI CACGAG 1 cut(s) 753
Bst2UI CCWGG 2 cut(s) 357, 2030
Bst4CI ACNGT 5 cut(s) 82, 1627, 1646, 1675, 1836
Bst6I CTCTTC 1 cut(s) 906
BstAUI TGTACA 2 cut(s) 226, 1364
BstBAI YACGTR 1 cut(s) 1898
BstBI TTCGAA 1 cut(s) 37
BstC8I GCNNGC 5 cut(s) 448, 942, 1217, 1268, 1553
BstDEI CTNAG 6 cut(s) 13, 114, 398, 1286, 1326, 1740
BstF5I GGATG 5 cut(s) 63, 369, 746, 861, 1265
BstFNI CGCG 1 cut(s) 1230
BstHHI GCGC 1 cut(s) 2072
BstKTI GATC 5 cut(s) 310, 369, 1285, 1339, 1856
BstMAI GTCTC 4 cut(s) 1395, 1420, 1706, 2018
BstMBI GATC 5 cut(s) 307, 366, 1282, 1336, 1853
BstMWI GCNNNNNNNGC 3 cut(s) 452, 986, 1874
BstNI CCWGG 2 cut(s) 357, 2030
BstNSI RCATGY 3 cut(s) 729, 1219, 2076
BstSCI CCNGG 3 cut(s) 355, 789, 2028
BstUI CGCG 1 cut(s) 1230
BstV1I GCAGC 3 cut(s) 141, 976, 1852
BstV2I GAAGAC 3 cut(s) 545, 1655, 1881
BstX2I RGATCY 1 cut(s) 1853
BstXI CCANNNNNNTGG 2 cut(s) 241, 500
BstYI RGATCY 1 cut(s) 1853
BstZ17I GTATAC 2 cut(s) 424, 767
Bsu15I ATCGAT 1 cut(s) 1984
BsuRI GGCC 1 cut(s) 783
BsuTUI ATCGAT 1 cut(s) 1984
BtgZI GCGATG 2 cut(s) 706, 995
BtsCI GGATG 5 cut(s) 63, 369, 746, 861, 1265
BtsI GCAGTG 2 cut(s) 784, 1464
BtsIMutI CAGTG 4 cut(s) 784, 1323, 1464, 1920
BveI ACCTGC 2 cut(s) 921, 1428
Cac8I GCNNGC 5 cut(s) 448, 942, 1217, 1268, 1553
CciI TCATGA 1 cut(s) 1342
CfoI GCGC 1 cut(s) 2072
Cfr13I GGNCC 1 cut(s) 353
ClaI ATCGAT 1 cut(s) 1984
CseI GACGC 2 cut(s) 1219, 1649
Csp6I GTAC 8 cut(s) 10, 107, 227, 273, 815, 869, 1365, 1915
CspCI CAANNNNNGTGG 2 cut(s) 1356, 1391
CviAII CATG 7 cut(s) 726, 860, 1154, 1216, 1343, 1822, 2073
CviQI GTAC 8 cut(s) 10, 107, 227, 273, 815, 869, 1365, 1915
DdeI CTNAG 6 cut(s) 13, 114, 398, 1286, 1326, 1740
DpnI GATC 5 cut(s) 309, 368, 1284, 1338, 1855
DpnII GATC 5 cut(s) 307, 366, 1282, 1336, 1853
DrdI GACNNNNNNGTC 1 cut(s) 1031
DseDI GACNNNNNNGTC 1 cut(s) 1031
EaeI YGGCCR 1 cut(s) 781
Eam1104I CTCTTC 1 cut(s) 906
EarI CTCTTC 1 cut(s) 906
Ecl136II GAGCTC 1 cut(s) 463
Eco130I CCWWGG 1 cut(s) 525
Eco24I GRGCYC 2 cut(s) 465, 918
Eco47I GGWCC 1 cut(s) 353
Eco53kI GAGCTC 1 cut(s) 463
Eco57I CTGAAG 4 cut(s) 930, 1424, 1535, 1984
Eco72I CACGTG 1 cut(s) 1898
EcoICRI GAGCTC 1 cut(s) 463
EcoRI GAATTC 1 cut(s) 1955
EcoRII CCWGG 2 cut(s) 355, 2028
EcoT14I CCWWGG 1 cut(s) 525
EcoT22I ATGCAT 1 cut(s) 861
EcoT38I GRGCYC 2 cut(s) 465, 918
ErhI CCWWGG 1 cut(s) 525
Esp3I CGTCTC 1 cut(s) 1395
FaeI CATG 7 cut(s) 729, 863, 1157, 1219, 1346, 1825, 2076
FalI AAGNNNNNCTT 2 cut(s) 177, 209
FaqI GGGAC 1 cut(s) 1984
FatI CATG 7 cut(s) 725, 859, 1153, 1215, 1342, 1821, 2072
FblI GTMKAC 4 cut(s) 423, 766, 1035, 1310
Fnu4HI GCNGC 4 cut(s) 130, 990, 1228, 1866
FokI GGATG 5 cut(s) 70, 356, 753, 868, 1272
FriOI GRGCYC 2 cut(s) 465, 918
Fsp4HI GCNGC 4 cut(s) 130, 990, 1228, 1866
FspBI CTAG 2 cut(s) 615, 675
GlaI GCGC 1 cut(s) 2071
GluI GCNGC 4 cut(s) 130, 990, 1228, 1866
GsuI CTGGAG 3 cut(s) 1299, 1577, 1787
HaeIII GGCC 1 cut(s) 783
HapII CCGG 1 cut(s) 791
HgaI GACGC 2 cut(s) 1219, 1649
HhaI GCGC 1 cut(s) 2072
Hin1II CATG 7 cut(s) 729, 863, 1157, 1219, 1346, 1825, 2076
Hin6I GCGC 1 cut(s) 2070
HinP1I GCGC 1 cut(s) 2070
HincII GTYRAC 1 cut(s) 1311
HindII GTYRAC 1 cut(s) 1311
HindIII AAGCTT 4 cut(s) 1369, 1549, 1772, 1875
HpaII CCGG 1 cut(s) 791
HphI GGTGA 3 cut(s) 442, 1287, 1376
Hpy166II GTNNAC 6 cut(s) 424, 767, 1036, 1295, 1311, 1384
Hpy188I TCNGA 6 cut(s) 1109, 1183, 1287, 1404, 1422, 1749
Hpy8I GTNNAC 6 cut(s) 424, 767, 1036, 1295, 1311, 1384
HpyAV CCTTC 6 cut(s) 116, 1063, 1463, 1529, 1605, 1844
HpyCH4III ACNGT 5 cut(s) 82, 1627, 1646, 1675, 1836
HpyCH4IV ACGT 1 cut(s) 1897
HpyCH4V TGCA 8 cut(s) 86, 129, 173, 620, 859, 1219, 1437, 1905
HpyF10VI GCNNNNNNNGC 3 cut(s) 452, 986, 1874
HpyF3I CTNAG 6 cut(s) 13, 114, 398, 1286, 1326, 1740
HpySE526I ACGT 1 cut(s) 1897
Hsp92II CATG 7 cut(s) 729, 863, 1157, 1219, 1346, 1825, 2076
HspAI GCGC 1 cut(s) 2070
Kzo9I GATC 5 cut(s) 307, 366, 1282, 1336, 1853
LguI GCTCTTC 1 cut(s) 906
LmnI GCTCC 4 cut(s) 1263, 1351, 1558, 1724
Lsp1109I GCAGC 3 cut(s) 141, 976, 1852
LweI GCATC 1 cut(s) 846
MaeI CTAG 2 cut(s) 615, 675
MaeII ACGT 1 cut(s) 1897
MaeIII GTNAC 4 cut(s) 757, 829, 1134, 2075
MalI GATC 5 cut(s) 309, 368, 1284, 1338, 1855
MboI GATC 5 cut(s) 307, 366, 1282, 1336, 1853
MfeI CAATTG 6 cut(s) 320, 392, 1041, 1602, 1779, 1860
MflI RGATCY 1 cut(s) 1853
MhlI GDGCHC 3 cut(s) 465, 918, 1611
MlsI TGGCCA 1 cut(s) 783
MluNI TGGCCA 1 cut(s) 783
MlyI GAGTC 3 cut(s) 1306, 1974, 2020
MmeI TCCRAC 1 cut(s) 1040
Mox20I TGGCCA 1 cut(s) 783
Mph1103I ATGCAT 1 cut(s) 861
MroXI GAANNNNTTC 1 cut(s) 1103
MscI TGGCCA 1 cut(s) 783
MseI TTAA 6 cut(s) 440, 483, 567, 669, 735, 1884
MslI CAYNNNNRTG 2 cut(s) 239, 1900
Msp20I TGGCCA 1 cut(s) 783
MspA1I CMGCKG 1 cut(s) 496
MspI CCGG 1 cut(s) 791
MspR9I CCNGG 3 cut(s) 357, 791, 2030
MunI CAATTG 6 cut(s) 320, 392, 1041, 1602, 1779, 1860
MvaI CCWGG 2 cut(s) 357, 2030
MvnI CGCG 1 cut(s) 1230
MwoI GCNNNNNNNGC 3 cut(s) 452, 986, 1874
NciI CCSGG 1 cut(s) 791
NdeII GATC 5 cut(s) 307, 366, 1282, 1336, 1853
NlaIII CATG 7 cut(s) 729, 863, 1157, 1219, 1346, 1825, 2076
NlaIV GGNNCC 3 cut(s) 580, 1332, 2059
NmuCI GTSAC 1 cut(s) 2075
NsiI ATGCAT 1 cut(s) 861
NspI RCATGY 3 cut(s) 729, 1219, 2076
NspV TTCGAA 1 cut(s) 37
PaeI GCATGC 1 cut(s) 1219
PagI TCATGA 1 cut(s) 1342
PciSI GCTCTTC 1 cut(s) 906
PdmI GAANNNNTTC 1 cut(s) 1103
PfeI GAWTC 8 cut(s) 39, 433, 1169, 1475, 1633, 1744, 1828, 1986
PkrI GCNGC 4 cut(s) 131, 991, 1229, 1867
PleI GAGTC 3 cut(s) 1306, 1973, 2019
PmaCI CACGTG 1 cut(s) 1898
PmlI CACGTG 1 cut(s) 1898
PpsI GAGTC 3 cut(s) 1306, 1973, 2019
Ppu21I YACGTR 1 cut(s) 1898
Psp124BI GAGCTC 1 cut(s) 465
Psp6I CCWGG 2 cut(s) 355, 2028
PspCI CACGTG 1 cut(s) 1898
PspGI CCWGG 2 cut(s) 355, 2028
PspN4I GGNNCC 3 cut(s) 580, 1332, 2059
PspPI GGNCC 1 cut(s) 353
PsuI RGATCY 1 cut(s) 1853
PvuII CAGCTG 1 cut(s) 496
RsaI GTAC 8 cut(s) 11, 108, 228, 274, 816, 870, 1366, 1916
RsaNI GTAC 8 cut(s) 10, 107, 227, 273, 815, 869, 1365, 1915
RseI CAYNNNNRTG 2 cut(s) 239, 1900
SacI GAGCTC 1 cut(s) 465
SalI GTCGAC 1 cut(s) 1309
SapI GCTCTTC 1 cut(s) 906
SaqAI TTAA 6 cut(s) 440, 483, 567, 669, 735, 1884
SatI GCNGC 4 cut(s) 130, 990, 1228, 1866
Sau3AI GATC 5 cut(s) 307, 366, 1282, 1336, 1853
Sau96I GGNCC 1 cut(s) 353
SchI GAGTC 3 cut(s) 1306, 1974, 2020
ScrFI CCNGG 3 cut(s) 357, 791, 2030
SduI GDGCHC 3 cut(s) 465, 918, 1611
SfaNI GCATC 1 cut(s) 846
SfuI TTCGAA 1 cut(s) 37
SinI GGWCC 1 cut(s) 353
SmiMI CAYNNNNRTG 2 cut(s) 239, 1900
SmlI CTYRAG 5 cut(s) 137, 458, 515, 1082, 1145
SmoI CTYRAG 5 cut(s) 137, 458, 515, 1082, 1145
SphI GCATGC 1 cut(s) 1219
SsiI CCGC 1 cut(s) 1228
SspI AATATT 3 cut(s) 711, 1361, 1488
SspMI CTAG 2 cut(s) 615, 675
SstI GAGCTC 1 cut(s) 465
StyD4I CCNGG 3 cut(s) 355, 789, 2028
StyI CCWWGG 1 cut(s) 525
TaaI ACNGT 5 cut(s) 82, 1627, 1646, 1675, 1836
TaiI ACGT 1 cut(s) 1900
TaqI TCGA 4 cut(s) 37, 970, 1310, 1984
TatI WGTACW 4 cut(s) 226, 272, 1364, 1914
TauI GCSGC 1 cut(s) 1230
TfiI GAWTC 8 cut(s) 39, 433, 1169, 1475, 1633, 1744, 1828, 1986
Tru1I TTAA 6 cut(s) 440, 483, 567, 669, 735, 1884
Tru9I TTAA 6 cut(s) 440, 483, 567, 669, 735, 1884
TscAI CASTG 4 cut(s) 791, 1330, 1471, 1927
TseFI GTSAC 1 cut(s) 2075
TseI GCWGC 3 cut(s) 129, 989, 1865
Tsp45I GTSAC 1 cut(s) 2075
TspGWI ACGGA 1 cut(s) 1870
TspRI CASTG 4 cut(s) 791, 1330, 1471, 1927
VpaK11BI GGWCC 1 cut(s) 353
XapI RAATTY 4 cut(s) 530, 1184, 1955, 2036
XceI RCATGY 3 cut(s) 729, 1219, 2076
XmiI GTMKAC 4 cut(s) 423, 766, 1035, 1310
XmnI GAANNNNTTC 1 cut(s) 1103
XspI CTAG 2 cut(s) 615, 675
Zsp2I ATGCAT 1 cut(s) 861
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.