Rmu_sc0005327.1_g000001

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005327.1
OG0000129
Physical Location & Seq
Reverse (-)
11778 .. 14900
3123 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005327.1_g000001.1.cds

Sequence Viewer

Length: 2724 bp
atgctacggcttattgatctgagtggttcacaatacttgatgtcgactccagatttcactgatgttcctgatcttgaaacgttggcgcttcaaaattgcacaagcttggtggagattcacccgtctcttggatttctgaagaaacttgcctctctggatatgagaaattgcaagtctattgagagcattccacctttcattacctccgaatcccttcaaatattgaggctttcactctgttcaggactgaagaagtttccagaaattgaaggaaatatcaaaagcttgctggagcttcatttggatgggaccaacattgaggaattgcctccatcaattgaacgcttgactggtcttaccatgttgaatctagcagactgtaaaaaccttttgcatcttcccaataccattgggtgcttgacatctttgaaaagtctcgatctaacaggttgctccaaaattgatgagatacctgagaatctgaatggtataaaatgtctggagatgctttgcattggtggaacttccataagggagttatctttcattgtaggcatgaagaatctacagtctctatcctgccgaggatgtgaaagtctagtgtcagaatcatgcaagggtctggcttcgttatcgagtttgatagagctagacttgagttattgcaatctgatggatggagcaattctcaatgattttagcagcctaatctccttggaattgttagatttaagtggtaattgctttgtgcgattacctgaaagtatctctcaactctcaaaacttgagactctgcgcttgaataattgcaaacagcttcaattgctgcccaacaagcttccgttaagtcttcgaaacgtgaatgcacaagattgtacttcactgacggattacccaaataaaatcaaattattgaattcatgggagtcgggagttactactgttaattccctcaattcttcagcagttaaagtctcacaaagtttttcaagtactgggggtgggggggaacttatacaagtgcgtatgacatttaatcatactgaggggaaacaagttgatcattttccagtgacagctgaaccgattctaaaacaattgcagacgtcttccaggtcaagttacatgagcgtatatgctggagatgaaattccagagtggttcaacactatatctaccaggaatcccatatcaatctcgatacctcaaaatctgaccgatgataacgagtggaacggatttgccgcgtgtgctatattctcagtcaaggggcatactgctctctcccttattgaaccggatcctgatttttctaattactcgtatcaaatcacattggaaactgatgttgtgcgtctggaaccatttgtgttgggagtggagtcagtggactttggcacctttggattaagttctcatctgcgtgtgattttcctggattctgcgtgtcggaatcatgctgtggacgccgcctctgtacgggagtctggctggcttagtcaacctttgaaatgggagaaattcattccacccttggaagaagaatacacacttgagctgagaaagaaccttgagtctgttcttccgagattccttgaggcactaaacagttatcatgtaacgttcgaatttaacctcagaggaagcccagcgtggtttccggaatttgtccttgatacggggtcaaattcgaacatagggccaatcaagctgcctcaaaatctacacaagagcaagaagtggatgggatttgcaatatacgcatcactggaagtggggcagacaatgaaagaggacaactattgggtatttgttacactatttggtacgaatgtggcccaaaaacttaagcacgtcaggccattgtcagaaaaacatcatcaactatttgttacttacataccgcgggcacagatgtccgaagtgttgttcactcagacattagaaactgcaatgtccattgtggtcgatagccctcatgtaaaggttcaaagatatgggttccgcattgtgtaccaagaagatatacaagggtttgctgatactattatccggtgcatgcagagggcagattctcttgaatctcacgataagttggtggtagaagactggataaagttgatacgattgcaaggtgcccatttagagagaatgacagaacgggattcccgaaccccacgggaaaaatactttgaattgcactcacaaaaatatagaaatcgggactggtctgaccctcgtcgtgcttcttgtatcttcaagggagttgaaatctccaagttgaaatggttcgtgccttttatcaaccaaggcaactcggcagaaatccaactgcctctgaatgtgttcgatgatgataattggttggggtttgccggatgttttcagttgttccacgaccaatgtctcggcagtactgctcctgattcagagaatgttatgcttatttaccgtctggcctctgatattgattggcagcgcgtatttgattcctacgagcaggtaagtttaaagattcgaaacccatcggcattcgaaatgactttgttcttttatacaccgcgcactagtcttagaaaggtttggaggcaatgcaaattggcaaggataactatgtggttgtctagaccatgcttcgcggtgcatagttgtgcccttcgtctactatataaggaggacgttgaacatcttgcagaaacactgaccctcgccgaattaccttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

907

Amino Acids

102.57

Weight (kDa)

5.57

Isoelectric Point (pI)

42.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1109
Acc36I ACCTGC 1 cut(s) 2491
AccB1I GGYRCC 2 cut(s) 1397, 2135
AccI GTMKAC 2 cut(s) 44, 2662
AccII CGCG 5 cut(s) 1247, 1906, 2481, 2563, 2639
AccIII TCCGGA 1 cut(s) 1660
AciI CCGC 7 cut(s) 1245, 1470, 1904, 1906, 2005, 2561, 2639
AclI AACGTT 2 cut(s) 80, 1622
AclWI GGATC 2 cut(s) 1295, 1308
AcsI RAATTY 6 cut(s) 916, 1149, 1520, 1628, 1664, 1687
AcuI CTGAAG 3 cut(s) 158, 269, 945
AcyI GRCGYC 2 cut(s) 1106, 1467
AfaI GTAC 6 cut(s) 877, 994, 1479, 1828, 2015, 2416
AfiI CCNNNNNNNGG 3 cut(s) 128, 1479, 1678
AflII CTTAAG 1 cut(s) 1847
AhlI ACTAGT 1 cut(s) 2567
AjiI CACGTC 1 cut(s) 1855
AjnI CCWGG 3 cut(s) 1112, 1178, 1434
AluBI AGCT 9 cut(s) 105, 285, 295, 649, 817, 838, 1079, 1558, 1711
AluI AGCT 9 cut(s) 105, 285, 295, 649, 817, 838, 1079, 1558, 1711
Alw26I GTCTC 6 cut(s) 129, 440, 576, 782, 979, 2411
AlwI GGATC 2 cut(s) 1295, 1308
Aor13HI TCCGGA 1 cut(s) 1660
AoxI GGCC 4 cut(s) 1700, 1836, 1859, 2457
ApeKI GCWGC 4 cut(s) 702, 826, 1711, 2476
ApoI RAATTY 6 cut(s) 916, 1149, 1520, 1628, 1664, 1687
Asp700I GAANNNNTTC 4 cut(s) 213, 1086, 2288, 2345
AspLEI GCGC 4 cut(s) 88, 798, 2481, 2565
AspS9I GGNCC 3 cut(s) 309, 1700, 1837
AsuHPI GGTGA 1 cut(s) 110
AsuII TTCGAA 5 cut(s) 853, 1626, 1691, 2518, 2535
AvaII GGWCC 1 cut(s) 309
BaeGI GKGCMC 3 cut(s) 1912, 2140, 2656
BaeI ACNNNNGTAYC 2 cut(s) 1818, 1851
BamHI GGATCC 1 cut(s) 1300
BanI GGYRCC 2 cut(s) 1397, 2135
BbsI GAAGAC 3 cut(s) 842, 1101, 2112
BbvI GCAGC 4 cut(s) 714, 813, 1698, 2488
BccI CCATC 6 cut(s) 299, 340, 667, 671, 1738, 2533
BceAI ACGGC 1 cut(s) 23
BciT130I CCWGG 3 cut(s) 1114, 1180, 1436
BclI TGATCA 1 cut(s) 1060
BcoDI GTCTC 6 cut(s) 129, 440, 576, 782, 979, 2411
BcuI ACTAGT 1 cut(s) 2567
BfaI CTAG 5 cut(s) 371, 599, 650, 2568, 2625
BfmI CTRYAG 1 cut(s) 566
BfoI RGCGCY 1 cut(s) 89
BfrI CTTAAG 1 cut(s) 1847
BfuAI ACCTGC 1 cut(s) 2491
BisI GCNGC 6 cut(s) 703, 827, 1245, 1470, 1712, 2477
BlsI GCNGC 6 cut(s) 704, 828, 1246, 1471, 1713, 2478
BmcAI AGTACT 2 cut(s) 994, 2416
Bme1390I CCNGG 3 cut(s) 1114, 1180, 1436
Bme18I GGWCC 1 cut(s) 309
BmgBI CACGTC 1 cut(s) 1855
BmgT120I GGNCC 3 cut(s) 309, 1700, 1837
BmiI GGNNCC 6 cut(s) 310, 1302, 1362, 1399, 2003, 2137
BmrFI CCNGG 3 cut(s) 1114, 1180, 1436
BmrI ACTGGG 1 cut(s) 1005
BmsI GCATC 3 cut(s) 403, 495, 1772
BmuI ACTGGG 1 cut(s) 1005
BoxI GACNNNNGTC 2 cut(s) 2238, 2403
BpiI GAAGAC 3 cut(s) 842, 1101, 2112
BplI GAGNNNNNCTC 2 cut(s) 672, 704
BpmI CTGGAG 4 cut(s) 33, 311, 521, 1161
Bpu14I TTCGAA 5 cut(s) 853, 1626, 1691, 2518, 2535
BpuEI CTTGAG 5 cut(s) 676, 806, 1574, 1592, 1616
BsaHI GRCGYC 2 cut(s) 1106, 1467
BsaJI CCNNGG 6 cut(s) 583, 714, 1533, 1904, 2177, 2308
BsaWI WCCGGW 3 cut(s) 1297, 1660, 2052
Bsc4I CCNNNNNNNGG 3 cut(s) 128, 1479, 1678
Bse1I ACTGG 6 cut(s) 355, 1000, 1070, 1773, 2114, 2231
Bse3DI GCAATG 2 cut(s) 1959, 2597
BseAI TCCGGA 1 cut(s) 1660
BseBI CCWGG 3 cut(s) 1114, 1180, 1436
BseDI CCNNGG 6 cut(s) 583, 714, 1533, 1904, 2177, 2308
BseGI GGATG 5 cut(s) 310, 593, 682, 1749, 2384
BseLI CCNNNNNNNGG 3 cut(s) 128, 1479, 1678
BseMI GCAATG 2 cut(s) 1959, 2597
BseMII CTCAG 7 cut(s) 11, 465, 1035, 1275, 1550, 1651, 1949
BseNI ACTGG 6 cut(s) 355, 1000, 1070, 1773, 2114, 2231
BseSI GKGCMC 3 cut(s) 1912, 2140, 2656
BseXI GCAGC 4 cut(s) 714, 813, 1698, 2488
BseYI CCCAGC 1 cut(s) 1648
Bsh1236I CGCG 5 cut(s) 1247, 1906, 2481, 2563, 2639
BshFI GGCC 4 cut(s) 1702, 1838, 1861, 2459
BshNI GGYRCC 2 cut(s) 1397, 2135
BsiSI CCGG 4 cut(s) 1298, 1661, 2053, 2376
BslFI GGGAC 2 cut(s) 322, 2237
BslI CCNNNNNNNGG 3 cut(s) 128, 1479, 1678
BsmAI GTCTC 6 cut(s) 129, 440, 576, 782, 979, 2411
BsmBI CGTCTC 1 cut(s) 129
BsmFI GGGAC 2 cut(s) 322, 2237
BsmI GAATGC 3 cut(s) 186, 868, 2531
BsnI GGCC 4 cut(s) 1702, 1838, 1861, 2459
Bsp119I TTCGAA 5 cut(s) 853, 1626, 1691, 2518, 2535
Bsp1286I GDGCHC 3 cut(s) 1912, 2140, 2656
Bsp13I TCCGGA 1 cut(s) 1660
Bsp143I GATC 5 cut(s) 16, 70, 439, 1060, 1300
BspACI CCGC 7 cut(s) 1245, 1470, 1904, 1906, 2005, 2561, 2639
BspANI GGCC 4 cut(s) 1702, 1838, 1861, 2459
BspCNI CTCAG 7 cut(s) 12, 466, 1036, 1274, 1551, 1650, 1948
BspEI TCCGGA 1 cut(s) 1660
BspFNI CGCG 5 cut(s) 1247, 1906, 2481, 2563, 2639
BspLI GGNNCC 6 cut(s) 310, 1302, 1362, 1399, 2003, 2137
BspMI ACCTGC 1 cut(s) 2491
BspPI GGATC 2 cut(s) 1295, 1308
BspT104I TTCGAA 5 cut(s) 853, 1626, 1691, 2518, 2535
BspT107I GGYRCC 2 cut(s) 1397, 2135
BspTI CTTAAG 1 cut(s) 1847
BsrDI GCAATG 2 cut(s) 1959, 2597
BsrI ACTGG 6 cut(s) 355, 1000, 1070, 1773, 2114, 2231
BssECI CCNNGG 6 cut(s) 583, 714, 1533, 1904, 2177, 2308
BssMI GATC 5 cut(s) 16, 70, 439, 1060, 1300
BssNI GRCGYC 2 cut(s) 1106, 1467
BssT1I CCWWGG 3 cut(s) 714, 1533, 2308
Bst2UI CCWGG 3 cut(s) 1114, 1180, 1436
Bst4CI ACNGT 5 cut(s) 380, 570, 943, 1610, 2453
BstACI GRCGYC 2 cut(s) 1106, 1467
BstAFI CTTAAG 1 cut(s) 1847
BstBI TTCGAA 5 cut(s) 853, 1626, 1691, 2518, 2535
BstC8I GCNNGC 4 cut(s) 287, 1493, 1908, 2060
BstDEI CTNAG 9 cut(s) 20, 474, 1044, 1261, 1496, 1559, 1637, 1935, 2573
BstDSI CCRYGG 2 cut(s) 1904, 2177
BstF5I GGATG 5 cut(s) 310, 593, 682, 1749, 2384
BstFNI CGCG 5 cut(s) 1247, 1906, 2481, 2563, 2639
BstH2I RGCGCY 1 cut(s) 89
BstHHI GCGC 4 cut(s) 88, 798, 2481, 2565
BstKTI GATC 5 cut(s) 19, 73, 442, 1063, 1303
BstMAI GTCTC 6 cut(s) 129, 440, 576, 782, 979, 2411
BstMBI GATC 5 cut(s) 16, 70, 439, 1060, 1300
BstMWI GCNNNNNNNGC 7 cut(s) 823, 835, 1250, 1466, 1708, 1760, 1858
BstNI CCWGG 3 cut(s) 1114, 1180, 1436
BstNSI RCATGY 1 cut(s) 2062
BstPAI GACNNNNGTC 2 cut(s) 2238, 2403
BstSCI CCNGG 3 cut(s) 1112, 1178, 1434
BstSFI CTRYAG 1 cut(s) 566
BstSLI GKGCMC 3 cut(s) 1912, 2140, 2656
BstUI CGCG 5 cut(s) 1247, 1906, 2481, 2563, 2639
BstV1I GCAGC 4 cut(s) 714, 813, 1698, 2488
BstV2I GAAGAC 3 cut(s) 842, 1101, 2112
BstX2I RGATCY 1 cut(s) 1300
BstYI RGATCY 1 cut(s) 1300
BsuRI GGCC 4 cut(s) 1702, 1838, 1861, 2459
BtgI CCRYGG 2 cut(s) 1904, 2177
BtrI CACGTC 1 cut(s) 1855
BtsCI GGATG 5 cut(s) 310, 593, 682, 1749, 2384
BtsIMutI CAGTG 6 cut(s) 57, 881, 1077, 1392, 1766, 2699
BveI ACCTGC 1 cut(s) 2491
Cac8I GCNNGC 4 cut(s) 287, 1493, 1908, 2060
CfoI GCGC 4 cut(s) 88, 798, 2481, 2565
Cfr13I GGNCC 3 cut(s) 309, 1700, 1837
Cfr42I CCGCGG 1 cut(s) 1907
CseI GACGC 2 cut(s) 1343, 1475
Csp6I GTAC 6 cut(s) 876, 993, 1478, 1827, 2014, 2415
CspCI CAANNNNNGTGG 2 cut(s) 90, 125
CviQI GTAC 6 cut(s) 876, 993, 1478, 1827, 2014, 2415
DdeI CTNAG 9 cut(s) 20, 474, 1044, 1261, 1496, 1559, 1637, 1935, 2573
DpnI GATC 5 cut(s) 18, 72, 441, 1062, 1302
DpnII GATC 5 cut(s) 16, 70, 439, 1060, 1300
DraI TTTAAA 1 cut(s) 2511
Eco130I CCWWGG 3 cut(s) 714, 1533, 2308
Eco47I GGWCC 1 cut(s) 309
Eco57I CTGAAG 3 cut(s) 158, 269, 945
EcoRI GAATTC 1 cut(s) 916
EcoRII CCWGG 3 cut(s) 1112, 1178, 1434
EcoT14I CCWWGG 3 cut(s) 714, 1533, 2308
ErhI CCWWGG 3 cut(s) 714, 1533, 2308
Esp3I CGTCTC 1 cut(s) 129
FaqI GGGAC 2 cut(s) 322, 2237
FauI CCCGC 1 cut(s) 1899
FbaI TGATCA 1 cut(s) 1060
FblI GTMKAC 2 cut(s) 44, 2662
Fnu4HI GCNGC 6 cut(s) 703, 827, 1245, 1470, 1712, 2477
FokI GGATG 5 cut(s) 317, 600, 689, 1756, 2391
Fsp4HI GCNGC 6 cut(s) 703, 827, 1245, 1470, 1712, 2477
FspBI CTAG 5 cut(s) 371, 599, 650, 2568, 2625
GlaI GCGC 4 cut(s) 87, 797, 2480, 2564
GluI GCNGC 6 cut(s) 703, 827, 1245, 1470, 1712, 2477
GsaI CCCAGC 1 cut(s) 1652
GsuI CTGGAG 4 cut(s) 33, 311, 521, 1161
HaeII RGCGCY 1 cut(s) 89
HaeIII GGCC 4 cut(s) 1702, 1838, 1861, 2459
HapII CCGG 4 cut(s) 1298, 1661, 2053, 2376
HgaI GACGC 2 cut(s) 1343, 1475
HhaI GCGC 4 cut(s) 88, 798, 2481, 2565
Hin1I GRCGYC 2 cut(s) 1106, 1467
Hin6I GCGC 4 cut(s) 86, 796, 2479, 2563
HinP1I GCGC 4 cut(s) 86, 796, 2479, 2563
HincII GTYRAC 2 cut(s) 45, 1502
HindII GTYRAC 2 cut(s) 45, 1502
HindIII AAGCTT 3 cut(s) 103, 283, 836
HpaII CCGG 4 cut(s) 1298, 1661, 2053, 2376
HphI GGTGA 1 cut(s) 110
Hpy166II GTNNAC 8 cut(s) 29, 45, 1390, 1465, 1502, 1932, 2014, 2663
Hpy8I GTNNAC 8 cut(s) 29, 45, 1390, 1465, 1502, 1932, 2014, 2663
Hpy99I CGWCG 1 cut(s) 2244
HpyAV CCTTC 3 cut(s) 224, 263, 2666
HpyCH4III ACNGT 5 cut(s) 380, 570, 943, 1610, 2453
HpyCH4IV ACGT 6 cut(s) 80, 858, 1106, 1622, 1854, 2679
HpyF10VI GCNNNNNNNGC 7 cut(s) 823, 835, 1250, 1466, 1708, 1760, 1858
HpyF3I CTNAG 9 cut(s) 20, 474, 1044, 1261, 1496, 1559, 1637, 1935, 2573
HpySE526I ACGT 6 cut(s) 80, 858, 1106, 1622, 1854, 2679
Hsp92I GRCGYC 2 cut(s) 1106, 1467
HspAI GCGC 4 cut(s) 86, 796, 2479, 2563
Kpn2I TCCGGA 1 cut(s) 1660
Ksp22I TGATCA 1 cut(s) 1060
KspI CCGCGG 1 cut(s) 1907
Kzo9I GATC 5 cut(s) 16, 70, 439, 1060, 1300
LmnI GCTCC 4 cut(s) 292, 458, 680, 2425
Lsp1109I GCAGC 4 cut(s) 714, 813, 1698, 2488
LweI GCATC 3 cut(s) 403, 495, 1772
MaeI CTAG 5 cut(s) 371, 599, 650, 2568, 2625
MaeII ACGT 6 cut(s) 80, 858, 1106, 1622, 1854, 2679
MaeIII GTNAC 6 cut(s) 934, 1072, 1121, 1618, 1813, 1891
MalI GATC 5 cut(s) 18, 72, 441, 1062, 1302
MboI GATC 5 cut(s) 16, 70, 439, 1060, 1300
MfeI CAATTG 3 cut(s) 336, 821, 1097
MflI RGATCY 1 cut(s) 1300
MhlI GDGCHC 3 cut(s) 1912, 2140, 2656
MlyI GAGTC 6 cut(s) 40, 784, 935, 1391, 1493, 1583
MmeI TCCRAC 2 cut(s) 1430, 2353
MroI TCCGGA 1 cut(s) 1660
MroXI GAANNNNTTC 4 cut(s) 213, 1086, 2288, 2345
MseI TTAA 9 cut(s) 731, 845, 945, 969, 1035, 1409, 1632, 1848, 2510
MslI CAYNNNNRTG 3 cut(s) 303, 1422, 2649
MspA1I CMGCKG 2 cut(s) 1079, 1906
MspCI CTTAAG 1 cut(s) 1847
MspI CCGG 4 cut(s) 1298, 1661, 2053, 2376
MspR9I CCNGG 3 cut(s) 1114, 1180, 1436
MunI CAATTG 3 cut(s) 336, 821, 1097
Mva1269I GAATGC 3 cut(s) 186, 868, 2531
MvaI CCWGG 3 cut(s) 1114, 1180, 1436
MvnI CGCG 5 cut(s) 1247, 1906, 2481, 2563, 2639
MwoI GCNNNNNNNGC 7 cut(s) 823, 835, 1250, 1466, 1708, 1760, 1858
NdeII GATC 5 cut(s) 16, 70, 439, 1060, 1300
NlaIV GGNNCC 6 cut(s) 310, 1302, 1362, 1399, 2003, 2137
NmeAIII GCCGAG 3 cut(s) 608, 2297, 2388
NmuCI GTSAC 1 cut(s) 1072
NspI RCATGY 1 cut(s) 2062
NspV TTCGAA 5 cut(s) 853, 1626, 1691, 2518, 2535
PaeI GCATGC 1 cut(s) 2062
PcsI WCGNNNNNNNCGW 1 cut(s) 2167
PctI GAATGC 3 cut(s) 186, 868, 2531
PdmI GAANNNNTTC 4 cut(s) 213, 1086, 2288, 2345
PfoI TCCNGGA 1 cut(s) 1434
PkrI GCNGC 6 cut(s) 704, 828, 1246, 1471, 1713, 2478
PleI GAGTC 6 cut(s) 40, 784, 934, 1390, 1492, 1582
PpsI GAGTC 6 cut(s) 40, 784, 934, 1390, 1492, 1582
PshAI GACNNNNGTC 2 cut(s) 2238, 2403
Psp1406I AACGTT 2 cut(s) 80, 1622
Psp6I CCWGG 3 cut(s) 1112, 1178, 1434
PspFI CCCAGC 1 cut(s) 1648
PspGI CCWGG 3 cut(s) 1112, 1178, 1434
PspN4I GGNNCC 6 cut(s) 310, 1302, 1362, 1399, 2003, 2137
PspPI GGNCC 3 cut(s) 309, 1700, 1837
PsuI RGATCY 1 cut(s) 1300
PvuII CAGCTG 1 cut(s) 1079
RsaI GTAC 6 cut(s) 877, 994, 1479, 1828, 2015, 2416
RsaNI GTAC 6 cut(s) 876, 993, 1478, 1827, 2014, 2415
RseI CAYNNNNRTG 3 cut(s) 303, 1422, 2649
SacII CCGCGG 1 cut(s) 1907
SalI GTCGAC 1 cut(s) 43
SaqAI TTAA 9 cut(s) 731, 845, 945, 969, 1035, 1409, 1632, 1848, 2510
SatI GCNGC 6 cut(s) 703, 827, 1245, 1470, 1712, 2477
Sau3AI GATC 5 cut(s) 16, 70, 439, 1060, 1300
Sau96I GGNCC 3 cut(s) 309, 1700, 1837
ScaI AGTACT 2 cut(s) 994, 2416
SchI GAGTC 6 cut(s) 40, 784, 935, 1391, 1493, 1583
ScrFI CCNGG 3 cut(s) 1114, 1180, 1436
SduI GDGCHC 3 cut(s) 1912, 2140, 2656
SfaNI GCATC 3 cut(s) 403, 495, 1772
SfcI CTRYAG 1 cut(s) 566
Sfr303I CCGCGG 1 cut(s) 1907
SfuI TTCGAA 5 cut(s) 853, 1626, 1691, 2518, 2535
SgrBI CCGCGG 1 cut(s) 1907
SinI GGWCC 1 cut(s) 309
SmiMI CAYNNNNRTG 3 cut(s) 303, 1422, 2649
SmlI CTYRAG 6 cut(s) 655, 785, 1553, 1571, 1595, 1847
SmoI CTYRAG 6 cut(s) 655, 785, 1553, 1571, 1595, 1847
SpeI ACTAGT 1 cut(s) 2567
SphI GCATGC 1 cut(s) 2062
SsiI CCGC 7 cut(s) 1245, 1470, 1904, 1906, 2005, 2561, 2639
SspI AATATT 1 cut(s) 222
SspMI CTAG 5 cut(s) 371, 599, 650, 2568, 2625
StyD4I CCNGG 3 cut(s) 1112, 1178, 1434
StyI CCWWGG 3 cut(s) 714, 1533, 2308
TaaI ACNGT 5 cut(s) 380, 570, 943, 1610, 2453
TaiI ACGT 6 cut(s) 83, 861, 1109, 1625, 1857, 2682
TaqII GACCGA 1 cut(s) 1232
TatI WGTACW 3 cut(s) 875, 992, 2414
TauI GCSGC 2 cut(s) 1247, 1472
Tru1I TTAA 9 cut(s) 731, 845, 945, 969, 1035, 1409, 1632, 1848, 2510
Tru9I TTAA 9 cut(s) 731, 845, 945, 969, 1035, 1409, 1632, 1848, 2510
TscAI CASTG 6 cut(s) 64, 888, 1077, 1392, 1773, 2706
TseFI GTSAC 1 cut(s) 1072
TseI GCWGC 4 cut(s) 702, 826, 1711, 2476
Tsp45I GTSAC 1 cut(s) 1072
TspDTI ATGAA 8 cut(s) 187, 287, 535, 572, 909, 1161, 1513, 1802
TspGWI ACGGA 3 cut(s) 831, 902, 1251
TspRI CASTG 6 cut(s) 64, 888, 1077, 1392, 1773, 2706
Vha464I CTTAAG 1 cut(s) 1847
VpaK11BI GGWCC 1 cut(s) 309
XapI RAATTY 6 cut(s) 916, 1149, 1520, 1628, 1664, 1687
XbaI TCTAGA 1 cut(s) 2624
XceI RCATGY 1 cut(s) 2062
XmiI GTMKAC 2 cut(s) 44, 2662
XmnI GAANNNNTTC 4 cut(s) 213, 1086, 2288, 2345
XspI CTAG 5 cut(s) 371, 599, 650, 2568, 2625
ZraI GACGTC 1 cut(s) 1107
ZrmI AGTACT 2 cut(s) 994, 2416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.