FvH4_5g38680

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000129
Physical Location & Seq
Forward (+)
28592523 .. 28599396
6874 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g38680.t5

Sequence Viewer

Length: 5040 bp
ATGCTCAGATTTCTCACACAGTTCGAACAAGTGATGGTCTCCTACCCAGACCGCCTTGTTCTCTTGTCTTCTATTTGCCCTCTGGTTTTCTTCTCTGCTTTGTTTCTCTTCAGCTTTATCTCTTCCGGGTTGTACCTGTTCTTCTTCTTCTCCACTGTTTCTATTTGCTGCTGCCTTTTGGCTTTCAGTCTTACCCATAACGAGAGACCACCAACACCCGACACTTGTTCAGTTACCACCAACACTGAAGTGATTCCTTCTTCTCCACGGTCCGGTAAACTCACCTACGATGTGTTCCTGAGTTTCAGAGGTGAAGATACCCGCAAGAATTTCACGGACCATCTCTACACTGCTCTCTTGCAGAAAGGGATACACACGTTTCGAGATGATGAAGAACTTGAGAGGGGGGAATCGATTGCTCCCAATCTCTTGAAAGCAATCGAAGAATCAAGATATGTCATTGTTGTCTTCTCACCAAACTACGCCGATTCTGCATGGTGTTTGGATGAGGTTGCCAAGGTCGCCGATTGCATGAAGGAAATGGGAACAAAAGTCCTTCCAGTGTTCTACCATGTTGATCCGTCGGAGGTACGAACACAAACAGGAGAGCACTTTGGGAAAGCATTTGAGAAGCATCAGAAGAGGTATAAAAAGGAACCAACCAAAGTACAGAGGTGGAAAGACGCTCTTTTCCAAGTAGGCAATCTCTCTGGATGGCACCTGCAAGATGGGTCTGAATCCAAAGTAATTCAAGAAATTGTAGAAAAGGTTTTCACTGAGATGAATAAAATAATCTCCACATCTGAGGGCTTAGTTGGGATGGATTCTCACCTGAATGAACTGTTTCCCTACTTAAACATTGGGGGTCTCGATGTTCGCATCATAGGGATTTGTGGAATGGGTGGTATTGGGAAGACTACTATTGCGCAAGTGGTTTTTGAAAGGATAAGAGCTCAGTTTGAAGGTGATAGCTTTCTTGAGAACGTTAAAAATGAAACTGAAAAGCAGGGTAGTACAGTTCATTTACAAGAGAAACTTCTCTTGAACTTGCTGAATACTAATGTCAATGTACAAAGCCCTAAAATGGGAAAAGACATTATTAAGCATAGACTATCTACTAAAAGGGTGCTTGTTGTTCTTGATGATGTGGATAAAGATGTACAATTGGAAACATTGTGTCACCGTACTTGGTTTGGTCCGGGGAGTAGAATCATAATAACATCCAGAGATGAACACTTGATCAGTGCATCTGGAGCAGATAAAGTATACATGGTGAATGCATTAACTGATCTTGAAGCTTTTGATCTCTTTCGTTTGAAAGCCTTCAATGAAGATGAGACTGGAGAAGATTTTCTCAAGCTATCCAATGAATTTTTGAAATATGCTAATGGCCTTCCATTAGCTATTAAAGTTTTGGGATCGTCTGTTCGTGGTAGAAATGTAAAGTTATGGTCAAGTGCATTGAAGAGACTTGAAAAAAATCCTCCAAAAGGAATTATTGATGTTCTTAAAGTCAGTTTTGATGGATTAGAAGAAACAGAAAAGAAAACCTTTTTAGACATCGCGTGTTTCTTTAAAGGATTGAACAAGGACCGTGTAATAAGAATACTAAGTGGTTGTGACGATGACGGTGTGGAGATGGATGTACAAGTTCTTATTGACAGATCTTTGGTAACTCTATTTCGAACAAATTTGTGGATGCATGATTTGATACAAGAAATGGGTCGTGAAGTTGTAATCCAAGAATGCCGTGAAGAGCCTGGAAAACGAAGCAGGTTGTGGCTTCTCAAGGAGATCATCAATGTACTAGGTCGGAGTAAGGCAACGAGTGCAGTCCAAAGCATATCCCTCCAATGTCCGGAAAAAGACAATGTCGTTCACTCAATCAATGATTCCTTCTCAAATATGGACGAATTGAGATTGCTCAAGATTTGGAATGTGAAATTTTCCGGGAACATCAACTATCTTTCGAATGAGTTACAGTATCTTGAATGGCATGACTGTCATTTGGATTCATTCCCGTCAAACTTTCAACCGGACAAACTCGTTGAAGTTCACATGCATTTTAGCCGCATCAAACAACTATGGAGGGGAAAACAAGGATGGAGCATGCTACGGTCTATATGTCTGAATGGTTCACGATATTTGATATCGACTCCAGACTTCACTGAAGTTCCTAATCTTGAAGTGTTGGCACTTCAAGATTGCACAAGCTTGGTGGAGATTCACCCGTCTCTTGGCTTTCTGAAGAAACTTATCTTTTTGAATATGGGAAACTGCAAGTCTATTGAGAGCCTTCCACCTCTCACTAGTTTGGAATCCCTTAAAAAACTGAGGCTTTCTCTGTGCTCAAAACTGAAGAAGTTTCCAGAAATTGAAGGGAATATGAAAAGCTTGCTGGAGCTTCATTTGGATGGGACCAGCATTGAGGAATTGCCTGCATCAATTGAACGCTTCACTGGTCTGTTAATGATGAATCTAAAAGACTGTAAAAACCTTTTGCGTCTTCCCAATAACATTGGGTGCTTGACATCTTTGAAAAGTCTCTCTCTAACAGGTTGCTCCAAAATTGATGAGATCCCCGAGAATCTGAATGGTATGAAATGTCTGGAGGAGCTTTTCATTGGCAGAACTTCCATAAGGGAGTTATCTTTCATTGCAGGCATGAAGAATCTACAGCGTCTATCCTGCCAAGGATGTACATGTCTAGTTTCAGAATCATGCAAAGGTCTGGCTTCATTATCGCGTTTGATATGGCTAGACTTGAGTTATTGCAATCTGATGGATGGAGCGTTTCTCAATTATTTTAGCAGCCTAATCTCCTTAAGACGGTTAAGCTTACGTGGTAATTGCTTTGTGCGATTACCTGAAAGTATCTCTCAACTCTCAAAGCTTGAGATTCTGGACTTGTGTAATTGTAGACAGCTTCTATTGCTGCCCAAGAACCTTCCGTTTAGTCTTCAGAACGTGTATGCACAAGACTGTACTTCACTGAAGGACTACCCAAACCAAATCAAAGTATTGAAATCATTCAATTCGCGAATGACTATTGTCAATTCACTCAATTCTTCAGCTCCTGGAATCTCACAAAATTCCGCAAGGACCGCAGCGTTGTATTTCTCAGTAGGTGAGGGGGAATATACTGTGACATATAAGCATCCAGAGGGAAAACAAGTTGATCATTTGCCACTGACAACACAAAGGCCGCCTCTCTTCTCAATTTCGAATTACAGTACTGTACTTCCTACAGATGAAATTCCAGAGTGGTTCACCACTATATCTACCAGAAATCCCATTTCAATTCTGATGCCTCAAAATGTGGCCAATGATAGCAAGTGGAAGGGATTTGCCGTGTGTGCTATATTCGCAGTCAAGGGGCATACAGCTCTATCCGTAATTGAACCGGATCCTGATTTCTGTAATTACTTGTATCAAGTCACATTGAAAACTGACGTTATGCGTCTTGAACCGGAAGTGTTGGATTGCACTGACCTAGATCGCAACCTTGGATCATGTTCTCATCTGCTTGTGATTTTTACTATATCTAGATTGACGTTTCCTTGGCGGTGGTTAATTGATTCAACTGAGGTGAGGGCTATATTTAAAACCACCAATCCATCCATGGTGGTTCAGAAATGTGGGGTTCATCTAGTGTATGAGCAAGATGTTCCATTAGTTAATTCCAGAATAGCCGATGGGGATCAAGCGATTTCTCTGGAAAAGGTTGAGGACTCTGCCTGGCTTAATCAATCTTTGAAATGGGAGAAATTCATTCCACCCTTGGAAGAAGAATCCACACTCGTGGCGAGAAAGAACCTTGAGTCTGTTCTTCCAAGATACATTGAGGGACTAAACAGTTATTCTGCATCCTACAAATTCAACCTCAGAGGAAGCCCTGTCTGGTTTCACGACTCGTGCCCCGTTATTCCGAGGTTAAATTTTGTTGCTGTTTCATTCAAGCTGCCTCAAAATCTACAGAAGAGCAAGAAGTGGATGGGATTTGCAATACATGCATCACTTGTAGAGGAAGTGGGGCAAATGGTGAAAGAGGACAACTATCGAGTAGTTTTTACACTACGTACTTGCAGTGATTCTCGTAAGACAGTGTCCCGAGAACTTAAGCACGTCAGGCCGTTGTCAGAAGAACATCATCAACTATTGGTTTTTTACATACCGCGGGCACTGCTTTCCGAAGTGTTGTTCACTCAGACATTAGCAACTACAATGGACTTAGACATTGAAATCATAACTGAGAGCCCTCATGTAAAGGTTCAAAGATTTGGGTTCCGTATTGTGTACGGGGAAGATATACAAGGGTTTTCTGATACGATTATCAGGTGCATGCAGAGGGAAGATTCTCTTAAATATTACGATAAGTTGGTAGTAGAGAAATGGATACATTTGGTGTGTACCTGCTGCATTAGCAGAGGTGCCCGTTTAGAGAGGATAACAGAACGGAATTCCAGAACCGCACAAGCAACCTTCAGAAATTGGGACTGGTCTGACCCTCGTCGTGCTGCTTGTTTGTTCGATAGAGTTGAAATCTCCAAGTTGAAATGGTTCGTGCCTTTCATCAACCAAGGCAACTCTGCCGAAATCCAACTGCCTCCGAATGTGTTTGATGATGATAACTGGTTGGGGTTTGCTGTATGTGTTCAGTGGTCCCGCTCTTCTTCTTCTTCAGAGGATGTTGTGCTTATTTCTCGACTGGACTCTGATGTTGCTTGGCACGGCTTATTTGATCACAAGTCGCATGTAATTATTGGGCTATTCGAGACACCTAAAGATGAATTATATGATGTGACTCGGTTCCATTATATACCGCGCGCTATAGTTAATAGTAAGATGTGGAGGCAATGCAAATTGGCCAGGATAACTGTGTGGTTCTCCAGCCCATGCGCAGCAGTAGTGCATACTTGTGCCCTTCGTCTACTATTTAAGGACGACATTGAACATCTAGTTGAGACACTCACTATTGCTGCATGTGACGAAAACCCTTATTTCATCCGTCTCCCTGCTGTGTTGAGTGAACCACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1680

Amino Acids

191.25

Weight (kDa)

6.48

Isoelectric Point (pI)

43.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 96 - 267 5.3e-56 TIR domain
TIR_2 PF13676 98 - 194 1.3e-17 TIR domain
NB-ARC PF00931 291 - 445 5.3e-22 NB-ARC domain
WHD_ROQ1 PF23282 511 - 579 1.5e-14 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 741 - 853 9.3e-09 Leucine-rich repeat region
LRR_13 PF23286 772 - 859 1.3e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 912 - 1011 3.3e-07 Leucine-rich repeat region
C-JID PF20160 1081 - 1223 1.3e-09 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 729
Acc16I TGCGCA 2 cut(s) 927, 4900
Acc36I ACCTGC 3 cut(s) 729, 1764, 4421
AccB1I GGYRCC 2 cut(s) 717, 4430
AccBSI CCGCTC 1 cut(s) 4668
AccI GTMKAC 3 cut(s) 1266, 2920, 4930
AccII CGCG 6 cut(s) 1565, 2746, 3040, 4177, 4825, 4827
AccIII TCCGGA 1 cut(s) 1858
AclI AACGTT 1 cut(s) 984
AclWI GGATC 7 cut(s) 572, 1426, 2572, 3401, 3414, 3517, 3708
AcoI YGGCCR 2 cut(s) 3321, 4866
AfiI CCNNNNNNNGG 7 cut(s) 272, 1084, 1085, 1490, 1858, 2237, 2829
AflII CTTAAG 2 cut(s) 2824, 4118
AflIII ACRYGT 3 cut(s) 375, 2702, 2967
AhlI ACTAGT 1 cut(s) 2309
AjiI CACGTC 1 cut(s) 4126
AjnI CCWGG 4 cut(s) 1759, 3076, 3737, 4868
AleI CACNNNNGTG 2 cut(s) 248, 3800
AloI GAACNNNNNNTCC 2 cut(s) 1410, 1442
Alw21I GWGCWC 3 cut(s) 612, 955, 2351
AlwI GGATC 7 cut(s) 572, 1426, 2572, 3401, 3414, 3517, 3708
AlwNI CAGNNNCTG 1 cut(s) 3077
Ama87I CYCGRG 2 cut(s) 2582, 4110
Aor13HI TCCGGA 1 cut(s) 1858
AoxI GGCC 5 cut(s) 1390, 3203, 3321, 4130, 4866
Asp700I GAANNNNTTC 6 cut(s) 252, 843, 1322, 1350, 3029, 4559
AspLEI GCGC 4 cut(s) 928, 4827, 4829, 4901
AspS9I GGNCC 7 cut(s) 270, 337, 1196, 1591, 2418, 3102, 4662
AsuC2I CCSGG 3 cut(s) 127, 1200, 1951
AsuII TTCGAA 4 cut(s) 24, 1684, 1970, 3224
AvaI CYCGRG 2 cut(s) 2582, 4110
AvaII GGWCC 7 cut(s) 270, 337, 1196, 1591, 2418, 3102, 4662
BaeGI GKGCMC 4 cut(s) 3920, 4183, 4435, 4924
BaeI ACNNNNGTAYC 2 cut(s) 362, 395
BalI TGGCCA 2 cut(s) 3323, 4868
BamHI GGATCC 1 cut(s) 3406
BanI GGYRCC 2 cut(s) 717, 4430
BanII GRGCYC 2 cut(s) 955, 4259
BarI GAAGNNNNNNTAC 2 cut(s) 125, 157
BauI CACGAG 2 cut(s) 3800, 3913
BbsI GAAGAC 5 cut(s) 60, 460, 920, 2498, 2951
Bbv12I GWGCWC 3 cut(s) 612, 955, 2351
BceAI ACGGC 4 cut(s) 1734, 3335, 4117, 4747
BciT130I CCWGG 4 cut(s) 1761, 3078, 3739, 4870
BciVI GTATCC 2 cut(s) 363, 4389
BclI TGATCA 3 cut(s) 1239, 3178, 4741
BcnI CCSGG 3 cut(s) 127, 1200, 1951
BcuI ACTAGT 1 cut(s) 2309
BfaI CTAG 8 cut(s) 1808, 2310, 2708, 2759, 3494, 3546, 3650, 4958
BfmI CTRYAG 4 cut(s) 2675, 3246, 3974, 4830
BfrI CTTAAG 2 cut(s) 2824, 4118
BfuAI ACCTGC 3 cut(s) 729, 1764, 4421
BfuI GTATCC 2 cut(s) 363, 4389
BglII AGATCT 1 cut(s) 1664
BmcAI AGTACT 1 cut(s) 3235
Bme1390I CCNGG 7 cut(s) 127, 1200, 1761, 1951, 3078, 3739, 4870
Bme18I GGWCC 7 cut(s) 270, 337, 1196, 1591, 2418, 3102, 4662
BmeT110I CYCGRG 2 cut(s) 2582, 4110
BmgBI CACGTC 1 cut(s) 4126
BmgT120I GGNCC 7 cut(s) 270, 337, 1196, 1591, 2418, 3102, 4662
BmiI GGNNCC 8 cut(s) 657, 719, 2419, 3408, 4286, 4432, 4664, 4811
BmrFI CCNGG 7 cut(s) 127, 1200, 1761, 1951, 3078, 3739, 4870
BoxI GACNNNNGTC 2 cut(s) 3050, 4509
BpiI GAAGAC 5 cut(s) 60, 460, 920, 2498, 2951
BplI GAGNNNNNCTC 4 cut(s) 2326, 2358, 2781, 2813
BpmI CTGGAG 6 cut(s) 1272, 1362, 2142, 2420, 2630, 4873
Bpu14I TTCGAA 4 cut(s) 24, 1684, 1970, 3224
BpuEI CTTGAG 8 cut(s) 419, 998, 1340, 1772, 1910, 2785, 2915, 3839
BpuMI CCSGG 3 cut(s) 127, 1200, 1951
Bsa29I ATCGAT 1 cut(s) 413
BsaAI YACGTR 2 cut(s) 2843, 4079
BsaBI GATNNNNATC 1 cut(s) 3699
BsaI GGTCTC 3 cut(s) 43, 199, 872
BsaWI WCCGGW 5 cut(s) 272, 1858, 2035, 3403, 3469
Bsc4I CCNNNNNNNGG 7 cut(s) 272, 1084, 1085, 1490, 1858, 2237, 2829
Bse1I ACTGG 6 cut(s) 560, 1345, 2464, 4502, 4637, 4713
Bse3DI GCAATG 2 cut(s) 2655, 4862
Bse8I GATNNNNATC 1 cut(s) 3699
BseAI TCCGGA 1 cut(s) 1858
BseBI CCWGG 4 cut(s) 1761, 3078, 3739, 4870
BseCI ATCGAT 1 cut(s) 413
BseJI GATNNNNATC 1 cut(s) 3699
BseLI CCNNNNNNNGG 7 cut(s) 272, 1084, 1085, 1490, 1858, 2237, 2829
BseMI GCAATG 2 cut(s) 2655, 4862
BseNI ACTGG 6 cut(s) 560, 1345, 2464, 4502, 4637, 4713
BsePI GCGCGC 1 cut(s) 4825
BseRI GAGGAG 1 cut(s) 2627
BseSI GKGCMC 4 cut(s) 3920, 4183, 4435, 4924
BsgI GTGCAG 1 cut(s) 1851
Bsh1236I CGCG 6 cut(s) 1565, 2746, 3040, 4177, 4825, 4827
BshFI GGCC 5 cut(s) 1392, 3205, 3323, 4132, 4868
BshNI GGYRCC 2 cut(s) 717, 4430
BshVI ATCGAT 1 cut(s) 413
BsiHKAI GWGCWC 3 cut(s) 612, 955, 2351
BsiHKCI CYCGRG 2 cut(s) 2582, 4110
BsiSI CCGG 8 cut(s) 126, 273, 1199, 1859, 1950, 2036, 3404, 3470
BslFI GGGAC 5 cut(s) 2431, 3861, 4093, 4508, 4648
BslI CCNNNNNNNGG 7 cut(s) 272, 1084, 1085, 1490, 1858, 2237, 2829
BsmBI CGTCTC 2 cut(s) 2238, 5015
BsmFI GGGAC 5 cut(s) 2431, 3861, 4093, 4508, 4648
BsmI GAATGC 2 cut(s) 1282, 1751
BsnI GGCC 5 cut(s) 1392, 3205, 3323, 4132, 4868
Bso31I GGTCTC 3 cut(s) 43, 199, 872
BsoBI CYCGRG 2 cut(s) 2582, 4110
Bsp119I TTCGAA 4 cut(s) 24, 1684, 1970, 3224
Bsp1286I GDGCHC 8 cut(s) 612, 955, 2351, 3920, 4183, 4259, 4435, 4924
Bsp13I TCCGGA 1 cut(s) 1858
Bsp1407I TGTACA 4 cut(s) 1069, 1159, 1645, 2699
Bsp19I CCATGG 1 cut(s) 3621
Bsp68I TCGCGA 1 cut(s) 3040
BspANI GGCC 5 cut(s) 1392, 3205, 3323, 4132, 4868
BspDI ATCGAT 1 cut(s) 413
BspEI TCCGGA 1 cut(s) 1858
BspFNI CGCG 6 cut(s) 1565, 2746, 3040, 4177, 4825, 4827
BspLI GGNNCC 8 cut(s) 657, 719, 2419, 3408, 4286, 4432, 4664, 4811
BspMI ACCTGC 3 cut(s) 729, 1764, 4421
BspPI GGATC 7 cut(s) 572, 1426, 2572, 3401, 3414, 3517, 3708
BspQI GCTCTTC 3 cut(s) 1749, 3974, 4675
BspT104I TTCGAA 4 cut(s) 24, 1684, 1970, 3224
BspT107I GGYRCC 2 cut(s) 717, 4430
BspTI CTTAAG 2 cut(s) 2824, 4118
BspTNI GGTCTC 3 cut(s) 43, 199, 872
BsrBI CCGCTC 1 cut(s) 4668
BsrDI GCAATG 2 cut(s) 2655, 4862
BsrGI TGTACA 4 cut(s) 1069, 1159, 1645, 2699
BsrI ACTGG 6 cut(s) 560, 1345, 2464, 4502, 4637, 4713
BssHII GCGCGC 1 cut(s) 4825
BssNAI GTATAC 1 cut(s) 1267
BssSI CACGAG 2 cut(s) 3800, 3913
BssT1I CCWWGG 7 cut(s) 516, 2692, 3505, 3560, 3621, 3780, 4579
Bst1107I GTATAC 1 cut(s) 1267
Bst2BI CACGAG 2 cut(s) 3800, 3913
Bst2UI CCWGG 4 cut(s) 1761, 3078, 3739, 4870
Bst6I CTCTTC 8 cut(s) 113, 127, 635, 1460, 1749, 3218, 3974, 4675
BstAFI CTTAAG 2 cut(s) 2824, 4118
BstAPI GCANNNNNTGC 2 cut(s) 1829, 4010
BstAUI TGTACA 4 cut(s) 1069, 1159, 1645, 2699
BstBAI YACGTR 2 cut(s) 2843, 4079
BstBI TTCGAA 4 cut(s) 24, 1684, 1970, 3224
BstC8I GCNNGC 7 cut(s) 2111, 2396, 2439, 2662, 4179, 4343, 4827
BstDSI CCRYGG 3 cut(s) 266, 3621, 4175
BstENI CCTNNNNNAGG 1 cut(s) 1488
BstFNI CGCG 6 cut(s) 1565, 2746, 3040, 4177, 4825, 4827
BstHHI GCGC 4 cut(s) 928, 4827, 4829, 4901
BstNI CCWGG 4 cut(s) 1761, 3078, 3739, 4870
BstNSI RCATGY 7 cut(s) 2062, 2113, 2706, 4013, 4345, 4757, 4986
BstPAI GACNNNNGTC 2 cut(s) 3050, 4509
BstSCI CCNGG 7 cut(s) 125, 1198, 1759, 1949, 3076, 3737, 4868
BstSFI CTRYAG 4 cut(s) 2675, 3246, 3974, 4830
BstSLI GKGCMC 4 cut(s) 3920, 4183, 4435, 4924
BstSNI TACGTA 1 cut(s) 4079
BstUI CGCG 6 cut(s) 1565, 2746, 3040, 4177, 4825, 4827
BstV2I GAAGAC 5 cut(s) 60, 460, 920, 2498, 2951
BstX2I RGATCY 3 cut(s) 1664, 2577, 3406
BstXI CCANNNNNNTGG 1 cut(s) 3802
BstYI RGATCY 3 cut(s) 1664, 2577, 3406
BstZ17I GTATAC 1 cut(s) 1267
Bsu15I ATCGAT 1 cut(s) 413
BsuI GTATCC 2 cut(s) 363, 4389
BsuRI GGCC 5 cut(s) 1392, 3205, 3323, 4132, 4868
BsuTUI ATCGAT 1 cut(s) 413
BtgI CCRYGG 3 cut(s) 266, 3621, 4175
BtgZI GCGATG 1 cut(s) 1546
BtrI CACGTC 1 cut(s) 4126
BtsI GCAGTG 3 cut(s) 348, 4093, 4181
BtuMI TCGCGA 1 cut(s) 3040
BveI ACCTGC 3 cut(s) 729, 1764, 4421
Cac8I GCNNGC 7 cut(s) 2111, 2396, 2439, 2662, 4179, 4343, 4827
CaiI CAGNNNCTG 1 cut(s) 3077
CfoI GCGC 4 cut(s) 928, 4827, 4829, 4901
Cfr13I GGNCC 7 cut(s) 270, 337, 1196, 1591, 2418, 3102, 4662
Cfr42I CCGCGG 1 cut(s) 4178
ClaI ATCGAT 1 cut(s) 413
CpoI CGGWCCG 1 cut(s) 270
CseI GACGC 4 cut(s) 692, 2492, 2669, 3449
CspCI CAANNNNNGTGG 2 cut(s) 2199, 2234
CspI CGGWCCG 1 cut(s) 270
DraI TTTAAA 2 cut(s) 1576, 3604
EaeI YGGCCR 2 cut(s) 3321, 4866
Eam1104I CTCTTC 8 cut(s) 113, 127, 635, 1460, 1749, 3218, 3974, 4675
EarI CTCTTC 8 cut(s) 113, 127, 635, 1460, 1749, 3218, 3974, 4675
Ecl136II GAGCTC 1 cut(s) 953
Eco105I TACGTA 1 cut(s) 4079
Eco130I CCWWGG 7 cut(s) 516, 2692, 3505, 3560, 3621, 3780, 4579
Eco24I GRGCYC 2 cut(s) 955, 4259
Eco31I GGTCTC 3 cut(s) 43, 199, 872
Eco32I GATATC 1 cut(s) 2151
Eco47I GGWCC 7 cut(s) 270, 337, 1196, 1591, 2418, 3102, 4662
Eco53kI GAGCTC 1 cut(s) 953
Eco88I CYCGRG 2 cut(s) 2582, 4110
EcoICRI GAGCTC 1 cut(s) 953
EcoNI CCTNNNNNAGG 1 cut(s) 1488
EcoRI GAATTC 1 cut(s) 4459
EcoRII CCWGG 4 cut(s) 1759, 3076, 3737, 4868
EcoRV GATATC 1 cut(s) 2151
EcoT14I CCWWGG 7 cut(s) 516, 2692, 3505, 3560, 3621, 3780, 4579
EcoT22I ATGCAT 4 cut(s) 1282, 1704, 2064, 4015
EcoT38I GRGCYC 2 cut(s) 955, 4259
ErhI CCWWGG 7 cut(s) 516, 2692, 3505, 3560, 3621, 3780, 4579
Esp3I CGTCTC 2 cut(s) 2238, 5015
FalI AAGNNNNNCTT 8 cut(s) 672, 704, 1020, 1052, 1535, 1567, 4344, 4376
FaqI GGGAC 5 cut(s) 2431, 3861, 4093, 4508, 4648
FauI CCCGC 3 cut(s) 329, 4170, 4673
FbaI TGATCA 3 cut(s) 1239, 3178, 4741
FblI GTMKAC 3 cut(s) 1266, 2920, 4930
FriOI GRGCYC 2 cut(s) 955, 4259
FspBI CTAG 8 cut(s) 1808, 2310, 2708, 2759, 3494, 3546, 3650, 4958
FspI TGCGCA 2 cut(s) 927, 4900
GlaI GCGC 4 cut(s) 927, 4826, 4828, 4900
GsuI CTGGAG 6 cut(s) 1272, 1362, 2142, 2420, 2630, 4873
HaeIII GGCC 5 cut(s) 1392, 3205, 3323, 4132, 4868
HapII CCGG 8 cut(s) 126, 273, 1199, 1859, 1950, 2036, 3404, 3470
HgaI GACGC 4 cut(s) 692, 2492, 2669, 3449
HhaI GCGC 4 cut(s) 928, 4827, 4829, 4901
Hin6I GCGC 4 cut(s) 926, 4825, 4827, 4899
HinP1I GCGC 4 cut(s) 926, 4825, 4827, 4899
HindIII AAGCTT 5 cut(s) 1296, 2212, 2392, 2836, 2891
HpaII CCGG 8 cut(s) 126, 273, 1199, 1859, 1950, 2036, 3404, 3470
Hpy99I CGWCG 2 cut(s) 586, 4515
HpyCH4IV ACGT 8 cut(s) 377, 984, 2842, 2967, 3453, 3554, 4078, 4125
HpySE526I ACGT 8 cut(s) 377, 984, 2842, 2967, 3453, 3554, 4078, 4125
HspAI GCGC 4 cut(s) 926, 4825, 4827, 4899
Kpn2I TCCGGA 1 cut(s) 1858
Ksp22I TGATCA 3 cut(s) 1239, 3178, 4741
KspI CCGCGG 1 cut(s) 4178
LguI GCTCTTC 3 cut(s) 1749, 3974, 4675
LmnI GCTCC 8 cut(s) 424, 1253, 2106, 2401, 2567, 2614, 2789, 3079
MaeI CTAG 8 cut(s) 1808, 2310, 2708, 2759, 3494, 3546, 3650, 4958
MaeII ACGT 8 cut(s) 377, 984, 2842, 2967, 3453, 3554, 4078, 4125
MaeIII GTNAC 9 cut(s) 232, 1178, 1619, 1672, 1977, 3145, 3436, 4801, 4985
MbiI CCGCTC 1 cut(s) 4668
MfeI CAATTG 2 cut(s) 1163, 2445
MflI RGATCY 3 cut(s) 1664, 2577, 3406
MhlI GDGCHC 8 cut(s) 612, 955, 2351, 3920, 4183, 4259, 4435, 4924
MlsI TGGCCA 2 cut(s) 3323, 4868
MluNI TGGCCA 2 cut(s) 3323, 4868
MlyI GAGTC 6 cut(s) 2149, 3725, 3830, 3905, 4706, 4798
MmeI TCCRAC 4 cut(s) 564, 1793, 3459, 4624
Mox20I TGGCCA 2 cut(s) 3323, 4868
Mph1103I ATGCAT 4 cut(s) 1282, 1704, 2064, 4015
MroI TCCGGA 1 cut(s) 1858
MroXI GAANNNNTTC 6 cut(s) 252, 843, 1322, 1350, 3029, 4559
MscI TGGCCA 2 cut(s) 3323, 4868
MslI CAYNNNNRTG 6 cut(s) 248, 779, 834, 2412, 3800, 4917
Msp20I TGGCCA 2 cut(s) 3323, 4868
MspA1I CMGCKG 1 cut(s) 4177
MspCI CTTAAG 2 cut(s) 2824, 4118
MspI CCGG 8 cut(s) 126, 273, 1199, 1859, 1950, 2036, 3404, 3470
MspR9I CCNGG 7 cut(s) 127, 1200, 1761, 1951, 3078, 3739, 4870
MunI CAATTG 2 cut(s) 1163, 2445
Mva1269I GAATGC 2 cut(s) 1282, 1751
MvaI CCWGG 4 cut(s) 1761, 3078, 3739, 4870
MvnI CGCG 6 cut(s) 1565, 2746, 3040, 4177, 4825, 4827
NciI CCSGG 3 cut(s) 127, 1200, 1951
NcoI CCATGG 1 cut(s) 3621
NlaIV GGNNCC 8 cut(s) 657, 719, 2419, 3408, 4286, 4432, 4664, 4811
NmuCI GTSAC 6 cut(s) 1178, 1619, 3145, 3436, 4801, 4985
NruI TCGCGA 1 cut(s) 3040
NsbI TGCGCA 2 cut(s) 927, 4900
NsiI ATGCAT 4 cut(s) 1282, 1704, 2064, 4015
NspI RCATGY 7 cut(s) 2062, 2113, 2706, 4013, 4345, 4757, 4986
NspV TTCGAA 4 cut(s) 24, 1684, 1970, 3224
OliI CACNNNNGTG 2 cut(s) 248, 3800
PaeI GCATGC 2 cut(s) 2113, 4345
PaqCI CACCTGC 1 cut(s) 729
PauI GCGCGC 1 cut(s) 4825
PciI ACATGT 1 cut(s) 2702
PciSI GCTCTTC 3 cut(s) 1749, 3974, 4675
PctI GAATGC 2 cut(s) 1282, 1751
PdmI GAANNNNTTC 6 cut(s) 252, 843, 1322, 1350, 3029, 4559
PflFI GACNNNGTC 2 cut(s) 1871, 4105
PfoI TCCNGGA 2 cut(s) 1949, 3076
PleI GAGTC 6 cut(s) 2149, 3725, 3829, 3905, 4706, 4798
PpsI GAGTC 6 cut(s) 2149, 3725, 3829, 3905, 4706, 4798
Ppu21I YACGTR 2 cut(s) 2843, 4079
PscI ACATGT 1 cut(s) 2702
PshAI GACNNNNGTC 2 cut(s) 3050, 4509
Psp124BI GAGCTC 1 cut(s) 955
Psp1406I AACGTT 1 cut(s) 984
Psp6I CCWGG 4 cut(s) 1759, 3076, 3737, 4868
PspGI CCWGG 4 cut(s) 1759, 3076, 3737, 4868
PspN4I GGNNCC 8 cut(s) 657, 719, 2419, 3408, 4286, 4432, 4664, 4811
PspPI GGNCC 7 cut(s) 270, 337, 1196, 1591, 2418, 3102, 4662
PstNI CAGNNNCTG 1 cut(s) 3077
PsuI RGATCY 3 cut(s) 1664, 2577, 3406
PsyI GACNNNGTC 2 cut(s) 1871, 4105
PteI GCGCGC 1 cut(s) 4825
RruI TCGCGA 1 cut(s) 3040
RseI CAYNNNNRTG 6 cut(s) 248, 779, 834, 2412, 3800, 4917
Rsr2I CGGWCCG 1 cut(s) 270
RsrII CGGWCCG 1 cut(s) 270
SacI GAGCTC 1 cut(s) 955
SacII CCGCGG 1 cut(s) 4178
SapI GCTCTTC 3 cut(s) 1749, 3974, 4675
Sau96I GGNCC 7 cut(s) 270, 337, 1196, 1591, 2418, 3102, 4662
ScaI AGTACT 1 cut(s) 3235
SchI GAGTC 6 cut(s) 2149, 3725, 3830, 3905, 4706, 4798
ScrFI CCNGG 7 cut(s) 127, 1200, 1761, 1951, 3078, 3739, 4870
SduI GDGCHC 8 cut(s) 612, 955, 2351, 3920, 4183, 4259, 4435, 4924
SfcI CTRYAG 4 cut(s) 2675, 3246, 3974, 4830
Sfr303I CCGCGG 1 cut(s) 4178
SfuI TTCGAA 4 cut(s) 24, 1684, 1970, 3224
SgrBI CCGCGG 1 cut(s) 4178
SinI GGWCC 7 cut(s) 270, 337, 1196, 1591, 2418, 3102, 4662
SmiMI CAYNNNNRTG 6 cut(s) 248, 779, 834, 2412, 3800, 4917
SnaBI TACGTA 1 cut(s) 4079
SpeI ACTAGT 1 cut(s) 2309
SphI GCATGC 2 cut(s) 2113, 4345
SspI AATATT 1 cut(s) 4367
SspMI CTAG 8 cut(s) 1808, 2310, 2708, 2759, 3494, 3546, 3650, 4958
SstI GAGCTC 1 cut(s) 955
StyD4I CCNGG 7 cut(s) 125, 1198, 1759, 1949, 3076, 3737, 4868
StyI CCWWGG 7 cut(s) 516, 2692, 3505, 3560, 3621, 3780, 4579
TaiI ACGT 8 cut(s) 380, 987, 2845, 2970, 3456, 3557, 4081, 4128
TauI GCSGC 2 cut(s) 2073, 3208
TseFI GTSAC 6 cut(s) 1178, 1619, 3145, 3436, 4801, 4985
Tsp45I GTSAC 6 cut(s) 1178, 1619, 3145, 3436, 4801, 4985
TspGWI ACGGA 7 cut(s) 350, 570, 2940, 3382, 4277, 4471, 4997
Tth111I GACNNNGTC 2 cut(s) 1871, 4105
Vha464I CTTAAG 2 cut(s) 2824, 4118
VpaK11BI GGWCC 7 cut(s) 270, 337, 1196, 1591, 2418, 3102, 4662
XagI CCTNNNNNAGG 1 cut(s) 1488
XbaI TCTAGA 1 cut(s) 3545
XceI RCATGY 7 cut(s) 2062, 2113, 2706, 4013, 4345, 4757, 4986
XcmI CCANNNNNNNNNTGG 1 cut(s) 3619
XmiI GTMKAC 3 cut(s) 1266, 2920, 4930
XmnI GAANNNNTTC 6 cut(s) 252, 843, 1322, 1350, 3029, 4559
XspI CTAG 8 cut(s) 1808, 2310, 2708, 2759, 3494, 3546, 3650, 4958
ZrmI AGTACT 1 cut(s) 3235
Zsp2I ATGCAT 4 cut(s) 1282, 1704, 2064, 4015
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.