Rmu_sc0001921.1_g000019

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001921.1
OG0000129
Physical Location & Seq
Forward (+)
114917 .. 120833
5917 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001921.1_g000019.1.cds

Sequence Viewer

Length: 4518 bp
atgactaccaaaatagatcttccttcttcttcttcgtcttcttccccaccatccggtaaattcacgtacgatgtgttcctcagtttcagaggtgaagatacccgcaaaaatttcacagaccatctttataccgctctcaaacagagaggaataaacacatttagagatgatgaagaacttaagagagggcaatcaattggtccaaatctcttgacagcaattgaagagtcaagatatgtaattgtggtgttttctcagaactacgccgattcttcgtggtgtttggatgagcttgcaaaagtagctgaatgcaggagagccatgggaaaaacagtccttccagtgttctaccatgttgagccgtttgaggtacgaagacaaaaaggcgatcgctttgagaaagcatttgagaagcatcaagagcggcatccagacaaattgcagaggtggaaagacgctcttgctgaagtaggcaatctctctggatggcatctgaaagatgggtacgaatccaaagtcattcaagaaattgttggaaatattttcaccaagttaaatcaattaatcccaatatcagagggtttagttgggatggattctcacctgaatgaaatgctttcgcacttagacattgggtgtccggatgttcgtatcatagggatttgtggcatgggtggtagcggtaagacaactattgcacaagtggtttttgaaagggtacaagctcagtttgaaggttgcagctttcttcagaatgtgagagagcagataactgaaaaacaagggatttgtggtatgggtggtatcggtaagacaactattgcacaagaggtttttgaaagggtacaagctcagtttgagggttgcagctttcttgagaatgtgagagagcagataactgaaaaacaaggtgcagttcatttacaacagcaacttctttcggatttgctgaaatgtagtgtaaatgtacagaacactaaaacgggaaaaaatataatgaggcagagactaagtactaaaatggttcttatcattcttgatgatgtggacaaagaaaaacaattggaagcattgtgtgataagaaatcggttggtccaggcagtagaatcatcataacttgtagagatgaacatttgctcagtgtagttgaaggagacaaagtatataaggtgaatccattaactgatgctgaagctcttcagcttttcagtatgaaagccttcaagaaagaccagctggttggaaaagaaattttgaagctatccaaagaatttttgaaatatgctaatggccttccactagctattgaagttttgggttgtcatgttaagggtagaggggtagagttatggtcaagtgcattggaaagacttaaagaaagtcctgataaagaaatttttaatgttctcaaggttagttttgatggattagaaccaagggaacagaaaatttttttggacatcgcatgtttccttaaaggggtgaacagagatcgtgtaagaagaatactacagggtagtaatgcccactgtccagactttgatatacaggttctcctggacaaatctatggtaactctatttggagaaaaattgtggatgcatgatttgatacaagaaatgggtcgagaagttgttcttcaagaatgccgtgaagagcctggaaaacgtagcagattgtggcttccaaatgagattatccatgtacttgatcggagtaagggatggagcatgctacggcatttagatctgactggttcagaagacttgacgtcgactccagacttcactgaggttcctgatcttgagacattggtgcttgaagactgtacaaacttggtggaggttcacccgtctcttggatttctgaagaaacttgtctctttgaatatgagaaattgcacgtctattgagagcattccaccgttcactgccttggaatcccttcaaatattgacactttcattctgttttggactgaagaagtttccagaaattgaaggaaatatgaaaagcctgctggagcttcatttggatgggaccaggattgaggaattgcctccatcaattgaacgcttgactggtcttaccatgttgaatctagcatactgtaataaccttttgcatcttcccaatgccattgggtgcttgacatctttgaaaaggctctatctaaaacattgctccaaaattgacgagatacctgagaatctgaatggtatgaaatgtctggagacgcttgcaattggtggaacttccataagggagttatctttcattctaggcatgaagaatctacagtatctatcctgccgaggatgtaaatgtctagtttcagaatcatgcaaaggtctggcttcgttatcgagtttaacgatgctagacttgagttattgcaatctgatggatggggcaattctcaatgattttagcagctccttaagagagttaaatttatgtggtaattgctttgtgcgattacctgaaagtatctctcaactctcaaagctcgagactctgcgcttgagtaactgcagacagcttcaattgctgcccaagaggcttccgttaagtcttcaaatagtgcatgcacaagattgtacttcactgacggattacccaaatcaaaacatactattgaattcaccagagtcaggagtgactattgtcaattccctcaactcttcagcactcggagtctcacaaggttctagaatgttgtctttttttaggcacggagaatctatggaccggtatgcgacattggaggggaaacaagttgatccaccgtcattgacaactccaacgattctaaaagaacagatttctccctcgtcaactttcatgggcgtatatcctcgagatgaaattccagagtggttcagcaccatatctaccagaaatcccatatcaatctcgatacctcaaaatctggccgatgataacaagtggaacggatttgccgcgtgtgctatattctcagtcaaggggcagactgctctctcccttattgaaccggatcctgattttcttaattactcgtatcaactcagattggaaactgatgttgggcttctgaaaccatatattgttggagcagtccctcccatgggattaagttctcatctgtttgtgattttccatatatctcgttcggagtttccacagaggtggttaaataaatcaactgtggtgagggctatatttgaaaccaccaatccatccatggtggtccagaaatgtggaatttgtctagtctatgatcaagatgctggatggcattccaggagcgttgattcgttttggctgaatgaagttaaggcccctgtacgggagtctggctggcgtagtccatctgtggaatgggagaaattcattccaccattggaagataaatccacacttgtgctaagaaagaacctcgagtctgttcttccaagatttcttgaggcactaaacagtgatgctgtatggttcgaatttaacctcatagaatgcccagcgtggttttggtcgtttgtccctaatacgaggtcagataagaccagagtgtcaatcaagctgcctcaaaatctacacaagagcaagaagtggatgggatttgcaatatacgcatcacttgtacaggaagtggggcagacaatgaaagaggacagttattgtgtatatgttggactacgaactggggatgaatctggtatgagtaattccaaagaacttgagcatgtcaagccattgtcagaagaacatcatcaactactggttatttacataccgcgagcacagattcccgaagcgtttttcactcagacattaacaactgcaatggactttctcatcatgatcagtgatagccctcatgtaaaggttcaaagatgtgggttccgtattgtgtaccaggaagatatacaagggtttgctgaaacaattatccagtgcatgcagaaggaagattctcttaaatctaacgaaaagctcatggtggaaaactggatacaggactggtttaaccctcgacctgttcattgtgtcttcgagggagctaaaatttccaagtggaaaaggttcatgcctttcatcaaccaaggcaactctgcagaaacccaactgcctctgaatgtgtttgatgatgataattggttgggttttgccgtatgttctcggttgtcccacaaccaatatccaaatattagtcctggcagtactgctcctgattcagaggatgttatgcttttttaccttctggactctgatgttgcttggcagcacttatttgatttcaagtttcaattatgtttaaagattcgagagccatcggaatacgatgcgacttggttcattcatataccgcgcaccgtatttcttggtgcaaagatatggaggcaatgcaaattggccaggataactatgtggttgtctagccaacgcttagcagtgtatagttgtggccttcgtctactatttaaggacgacgttgaacgtcttgtagaaacactgaccctcgccgaattaccttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1505

Amino Acids

170.66

Weight (kDa)

6.01

Isoelectric Point (pI)

45.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 3550
AatII GACGTC 1 cut(s) 1775
AccBSI CCGCTC 2 cut(s) 134, 424
AccI GTMKAC 2 cut(s) 1775, 4456
AccII CGCG 3 cut(s) 2966, 3778, 4351
AccIII TCCGGA 1 cut(s) 640
AciI CCGC 7 cut(s) 103, 132, 424, 681, 2964, 3776, 4349
AclWI GGATC 3 cut(s) 2777, 3014, 3027
AcoI YGGCCR 2 cut(s) 2934, 4395
AcuI CTGAAG 7 cut(s) 486, 734, 1184, 1212, 1889, 2000, 2670
AcyI GRCGYC 1 cut(s) 1772
AfiI CCNNNNNNNGG 8 cut(s) 53, 1480, 1859, 2654, 3109, 3110, 3330, 3331
AflII CTTAAG 2 cut(s) 179, 2440
AgeI ACCGGT 1 cut(s) 2751
AhdI GACNNNNNGTC 1 cut(s) 1771
AjiI CACGTC 1 cut(s) 1905
AjnI CCWGG 8 cut(s) 1096, 1557, 1660, 2042, 3284, 3897, 4195, 4397
AjuI GAANNNNNNNTTGG 4 cut(s) 1439, 1471, 3051, 3083
AleI CACNNNNGTG 2 cut(s) 3169, 3404
Alw21I GWGCWC 1 cut(s) 3784
Alw26I GTCTC 8 cut(s) 1000, 1149, 1802, 1860, 1885, 2228, 2507, 2704
AlwI GGATC 3 cut(s) 2777, 3014, 3027
Ama87I CYCGRG 3 cut(s) 2510, 2859, 3422
Aor13HI TCCGGA 1 cut(s) 640
AoxI GGCC 5 cut(s) 1291, 2934, 3321, 4395, 4447
ApeKI GCWGC 6 cut(s) 741, 867, 2433, 2551, 3562, 4264
ArsI GACNNNNNNTTYG 2 cut(s) 501, 533
AseI ATTAAT 1 cut(s) 563
AsiGI ACCGGT 1 cut(s) 2751
AsiSI GCGATCGC 1 cut(s) 391
Asp700I GAANNNNTTC 5 cut(s) 1197, 1220, 1635, 1944, 4064
AspLEI GCGC 2 cut(s) 2523, 4353
AspS9I GGNCC 6 cut(s) 200, 1094, 2040, 2749, 3232, 3322
AsuHPI GGTGA 8 cut(s) 104, 538, 593, 1183, 1495, 1841, 2637, 3205
AsuII TTCGAA 1 cut(s) 3477
AvaI CYCGRG 3 cut(s) 2510, 2859, 3422
AvaII GGWCC 5 cut(s) 200, 1094, 2040, 2749, 3232
BalI TGGCCA 1 cut(s) 4397
BamHI GGATCC 1 cut(s) 3019
BbsI GAAGAC 6 cut(s) 30, 382, 1770, 1830, 2567, 4024
Bbv12I GWGCWC 1 cut(s) 3784
BbvI GCAGC 6 cut(s) 753, 879, 2445, 2538, 3549, 4276
BceAI ACGGC 4 cut(s) 346, 1635, 1754, 4136
BciT130I CCWGG 8 cut(s) 1098, 1559, 1662, 2044, 3286, 3899, 4197, 4399
BciVI GTATCC 1 cut(s) 3987
BclI TGATCA 2 cut(s) 3262, 3843
BcoDI GTCTC 8 cut(s) 1000, 1149, 1802, 1860, 1885, 2228, 2507, 2704
BfaI CTAG 8 cut(s) 1301, 2102, 2282, 2330, 2381, 2712, 3254, 4419
BfmI CTRYAG 4 cut(s) 1511, 2297, 2533, 4095
BfrI CTTAAG 2 cut(s) 179, 2440
BfuI GTATCC 1 cut(s) 3987
BglI GCCNNNNNGGC 1 cut(s) 2560
BglII AGATCT 2 cut(s) 16, 1747
BisI GCNGC 8 cut(s) 425, 742, 868, 2434, 2552, 2964, 3563, 4265
BlpI GCTNAGC 1 cut(s) 4429
BlsI GCNGC 8 cut(s) 426, 743, 869, 2435, 2553, 2965, 3564, 4266
BmcAI AGTACT 2 cut(s) 1015, 4204
Bme1390I CCNGG 8 cut(s) 1098, 1559, 1662, 2044, 3286, 3899, 4197, 4399
Bme18I GGWCC 5 cut(s) 200, 1094, 2040, 2749, 3232
BmeRI GACNNNNNGTC 1 cut(s) 1771
BmeT110I CYCGRG 3 cut(s) 2510, 2859, 3422
BmgBI CACGTC 1 cut(s) 1905
BmgT120I GGNCC 6 cut(s) 200, 1094, 2040, 2749, 3232, 3322
BmiI GGNNCC 5 cut(s) 1797, 2041, 3021, 3324, 3884
BmrFI CCNGG 8 cut(s) 1098, 1559, 1662, 2044, 3286, 3899, 4197, 4399
BmrI ACTGGG 1 cut(s) 3693
BmuI ACTGGG 1 cut(s) 3693
BoxI GACNNNNGTC 1 cut(s) 2666
BpiI GAAGAC 6 cut(s) 30, 382, 1770, 1830, 2567, 4024
BpmI CTGGAG 3 cut(s) 1764, 2042, 2252
Bpu1102I GCTNAGC 1 cut(s) 4429
Bpu14I TTCGAA 1 cut(s) 3477
BpuEI CTTGAG 7 cut(s) 896, 1394, 1826, 2407, 2545, 3467, 3740
BsaAI YACGTR 1 cut(s) 66
BsaHI GRCGYC 1 cut(s) 1772
BsaJI CCNNGG 7 cut(s) 321, 1436, 1935, 2314, 3108, 3225, 4084
BsaWI WCCGGW 4 cut(s) 53, 640, 2751, 3016
BsaXI ACNNNNNCTCC 6 cut(s) 1146, 1176, 1539, 1569, 1762, 1792
Bsc4I CCNNNNNNNGG 8 cut(s) 53, 1480, 1859, 2654, 3109, 3110, 3330, 3331
Bse118I RCCGGY 1 cut(s) 2751
Bse1I ACTGG 8 cut(s) 341, 1759, 2086, 3688, 3765, 3934, 3995, 4007
Bse3DI GCAATG 3 cut(s) 2179, 3831, 4391
BseAI TCCGGA 1 cut(s) 640
BseBI CCWGG 8 cut(s) 1098, 1559, 1662, 2044, 3286, 3899, 4197, 4399
BseDI CCNNGG 7 cut(s) 321, 1436, 1935, 2314, 3108, 3225, 4084
BseLI CCNNNNNNNGG 8 cut(s) 53, 1480, 1859, 2654, 3109, 3110, 3330, 3331
BseMI GCAATG 3 cut(s) 2179, 3831, 4391
BseNI ACTGG 8 cut(s) 341, 1759, 2086, 3688, 3765, 3934, 3995, 4007
BseXI GCAGC 6 cut(s) 753, 879, 2445, 2538, 3549, 4276
BseYI CCCAGC 1 cut(s) 3499
BsgI GTGCAG 1 cut(s) 933
Bsh1236I CGCG 3 cut(s) 2966, 3778, 4351
Bsh1285I CGRYCG 1 cut(s) 391
BshFI GGCC 5 cut(s) 1293, 2936, 3323, 4397, 4449
BshTI ACCGGT 1 cut(s) 2751
BsiEI CGRYCG 1 cut(s) 391
BsiHKAI GWGCWC 1 cut(s) 3784
BsiHKCI CYCGRG 3 cut(s) 2510, 2859, 3422
BsiSI CCGG 4 cut(s) 54, 641, 2752, 3017
BsiWI CGTACG 1 cut(s) 66
BslFI GGGAC 4 cut(s) 2053, 3086, 3506, 4153
BslI CCNNNNNNNGG 8 cut(s) 53, 1480, 1859, 2654, 3109, 3110, 3330, 3331
BsmAI GTCTC 8 cut(s) 1000, 1149, 1802, 1860, 1885, 2228, 2507, 2704
BsmBI CGTCTC 2 cut(s) 1860, 2228
BsmFI GGGAC 4 cut(s) 2053, 3086, 3506, 4153
BsmI GAATGC 5 cut(s) 314, 1652, 1917, 3280, 3500
BsnI GGCC 5 cut(s) 1293, 2936, 3323, 4397, 4449
BsoBI CYCGRG 3 cut(s) 2510, 2859, 3422
Bsp119I TTCGAA 1 cut(s) 3477
Bsp1286I GDGCHC 1 cut(s) 3784
Bsp13I TCCGGA 1 cut(s) 640
Bsp1407I TGTACA 3 cut(s) 967, 1829, 3622
Bsp1720I GCTNAGC 1 cut(s) 4429
Bsp19I CCATGG 3 cut(s) 321, 3108, 3225
BspACI CCGC 7 cut(s) 103, 132, 424, 681, 2964, 3776, 4349
BspANI GGCC 5 cut(s) 1293, 2936, 3323, 4397, 4449
BspEI TCCGGA 1 cut(s) 640
BspFNI CGCG 3 cut(s) 2966, 3778, 4351
BspHI TCATGA 1 cut(s) 3840
BspLI GGNNCC 5 cut(s) 1797, 2041, 3021, 3324, 3884
BspMAI CTGCAG 2 cut(s) 2537, 4099
BspPI GGATC 3 cut(s) 2777, 3014, 3027
BspQI GCTCTTC 2 cut(s) 1203, 1650
BspT104I TTCGAA 1 cut(s) 3477
BspTI CTTAAG 2 cut(s) 179, 2440
BsrBI CCGCTC 2 cut(s) 134, 424
BsrDI GCAATG 3 cut(s) 2179, 3831, 4391
BsrFI RCCGGY 1 cut(s) 2751
BsrGI TGTACA 3 cut(s) 967, 1829, 3622
BsrI ACTGG 8 cut(s) 341, 1759, 2086, 3688, 3765, 3934, 3995, 4007
BssAI RCCGGY 1 cut(s) 2751
BssECI CCNNGG 7 cut(s) 321, 1436, 1935, 2314, 3108, 3225, 4084
BssNI GRCGYC 1 cut(s) 1772
BssT1I CCWWGG 6 cut(s) 321, 1436, 1935, 3108, 3225, 4084
Bst2UI CCWGG 8 cut(s) 1098, 1559, 1662, 2044, 3286, 3899, 4197, 4399
Bst6I CTCTTC 4 cut(s) 219, 1203, 1650, 2689
BstACI GRCGYC 1 cut(s) 1772
BstAFI CTTAAG 2 cut(s) 179, 2440
BstAUI TGTACA 3 cut(s) 967, 1829, 3622
BstBAI YACGTR 1 cut(s) 66
BstBI TTCGAA 1 cut(s) 3477
BstC8I GCNNGC 8 cut(s) 294, 1733, 2018, 2241, 2589, 3344, 3780, 3941
BstDSI CCRYGG 3 cut(s) 321, 3108, 3225
BstFNI CGCG 3 cut(s) 2966, 3778, 4351
BstHHI GCGC 2 cut(s) 2523, 4353
BstMAI GTCTC 8 cut(s) 1000, 1149, 1802, 1860, 1885, 2228, 2507, 2704
BstMCI CGRYCG 1 cut(s) 391
BstMWI GCNNNNNNNGC 7 cut(s) 302, 421, 2548, 2560, 2969, 3611, 3730
BstNI CCWGG 8 cut(s) 1098, 1559, 1662, 2044, 3286, 3899, 4197, 4399
BstNSI RCATGY 5 cut(s) 1470, 1735, 2591, 3728, 3943
BstPAI GACNNNNGTC 1 cut(s) 2666
BstSCI CCNGG 8 cut(s) 1096, 1557, 1660, 2042, 3284, 3897, 4195, 4397
BstSFI CTRYAG 4 cut(s) 1511, 2297, 2533, 4095
BstUI CGCG 3 cut(s) 2966, 3778, 4351
BstV1I GCAGC 6 cut(s) 753, 879, 2445, 2538, 3549, 4276
BstV2I GAAGAC 6 cut(s) 30, 382, 1770, 1830, 2567, 4024
BstX2I RGATCY 3 cut(s) 16, 1747, 3019
BstXI CCANNNNNNTGG 3 cut(s) 1241, 3171, 3242
BstYI RGATCY 3 cut(s) 16, 1747, 3019
BsuI GTATCC 1 cut(s) 3987
BsuRI GGCC 5 cut(s) 1293, 2936, 3323, 4397, 4449
BtgI CCRYGG 3 cut(s) 321, 3108, 3225
BtgZI GCGATG 1 cut(s) 1447
BtrI CACGTC 1 cut(s) 1905
BtsI GCAGTG 2 cut(s) 1929, 4440
Cac8I GCNNGC 8 cut(s) 294, 1733, 2018, 2241, 2589, 3344, 3780, 3941
CciI TCATGA 1 cut(s) 3840
CfoI GCGC 2 cut(s) 2523, 4353
Cfr10I RCCGGY 1 cut(s) 2751
Cfr13I GGNCC 6 cut(s) 200, 1094, 2040, 2749, 3232, 3322
CseI GACGC 2 cut(s) 464, 2245
CspAI ACCGGT 1 cut(s) 2751
CspCI CAANNNNNGTGG 4 cut(s) 1821, 1856, 2775, 2810
DraI TTTAAA 1 cut(s) 4299
DrdI GACNNNNNNGTC 1 cut(s) 3550
DriI GACNNNNNGTC 1 cut(s) 1771
DseDI GACNNNNNNGTC 1 cut(s) 3550
EaeI YGGCCR 2 cut(s) 2934, 4395
Eam1104I CTCTTC 4 cut(s) 219, 1203, 1650, 2689
Eam1105I GACNNNNNGTC 1 cut(s) 1771
EarI CTCTTC 4 cut(s) 219, 1203, 1650, 2689
Eco130I CCWWGG 6 cut(s) 321, 1436, 1935, 3108, 3225, 4084
Eco47I GGWCC 5 cut(s) 200, 1094, 2040, 2749, 3232
Eco57I CTGAAG 7 cut(s) 486, 734, 1184, 1212, 1889, 2000, 2670
Eco88I CYCGRG 3 cut(s) 2510, 2859, 3422
EcoO109I RGGNCCY 1 cut(s) 3322
EcoRI GAATTC 1 cut(s) 2641
EcoRII CCWGG 8 cut(s) 1096, 1557, 1660, 2042, 3284, 3897, 4195, 4397
EcoT14I CCWWGG 6 cut(s) 321, 1436, 1935, 3108, 3225, 4084
EcoT22I ATGCAT 1 cut(s) 1605
ErhI CCWWGG 6 cut(s) 321, 1436, 1935, 3108, 3225, 4084
Esp3I CGTCTC 2 cut(s) 1860, 2228
FalI AAGNNNNNCTT 6 cut(s) 444, 476, 1623, 1655, 3942, 3974
FaqI GGGAC 4 cut(s) 2053, 3086, 3506, 4153
FauI CCCGC 1 cut(s) 110
FbaI TGATCA 2 cut(s) 3262, 3843
FblI GTMKAC 2 cut(s) 1775, 4456
Fnu4HI GCNGC 8 cut(s) 425, 742, 868, 2434, 2552, 2964, 3563, 4265
Fsp4HI GCNGC 8 cut(s) 425, 742, 868, 2434, 2552, 2964, 3563, 4265
FspBI CTAG 8 cut(s) 1301, 2102, 2282, 2330, 2381, 2712, 3254, 4419
GlaI GCGC 2 cut(s) 2522, 4352
GluI GCNGC 8 cut(s) 425, 742, 868, 2434, 2552, 2964, 3563, 4265
GsaI CCCAGC 1 cut(s) 3503
GsuI CTGGAG 3 cut(s) 1764, 2042, 2252
HaeIII GGCC 5 cut(s) 1293, 2936, 3323, 4397, 4449
HapII CCGG 4 cut(s) 54, 641, 2752, 3017
HgaI GACGC 2 cut(s) 464, 2245
HhaI GCGC 2 cut(s) 2523, 4353
Hin1I GRCGYC 1 cut(s) 1772
Hin6I GCGC 2 cut(s) 2521, 4351
HinP1I GCGC 2 cut(s) 2521, 4351
HincII GTYRAC 2 cut(s) 1776, 2838
HindII GTYRAC 2 cut(s) 1776, 2838
HpaII CCGG 4 cut(s) 54, 641, 2752, 3017
HphI GGTGA 8 cut(s) 104, 538, 593, 1183, 1495, 1841, 2637, 3205
Hpy166II GTNNAC 8 cut(s) 1048, 1486, 1776, 1849, 1929, 2838, 3895, 4457
Hpy8I GTNNAC 8 cut(s) 1048, 1486, 1776, 1849, 1929, 2838, 3895, 4457
Hpy99I CGWCG 2 cut(s) 1777, 4475
HpyCH4IV ACGT 6 cut(s) 65, 1669, 1772, 1904, 4473, 4480
HpyF10VI GCNNNNNNNGC 7 cut(s) 302, 421, 2548, 2560, 2969, 3611, 3730
HpySE526I ACGT 6 cut(s) 65, 1669, 1772, 1904, 4473, 4480
Hsp92I GRCGYC 1 cut(s) 1772
HspAI GCGC 2 cut(s) 2521, 4351
Kpn2I TCCGGA 1 cut(s) 640
Ksp22I TGATCA 2 cut(s) 3262, 3843
LguI GCTCTTC 2 cut(s) 1203, 1650
LmnI GCTCC 8 cut(s) 1728, 2023, 2189, 2441, 3095, 3288, 4040, 4213
Lsp1109I GCAGC 6 cut(s) 753, 879, 2445, 2538, 3549, 4276
MaeI CTAG 8 cut(s) 1301, 2102, 2282, 2330, 2381, 2712, 3254, 4419
MaeII ACGT 6 cut(s) 65, 1669, 1772, 1904, 4473, 4480
MaeIII GTNAC 3 cut(s) 1573, 2528, 2659
MbiI CCGCTC 2 cut(s) 134, 424
MfeI CAATTG 6 cut(s) 195, 219, 1061, 2067, 2244, 2546
MflI RGATCY 3 cut(s) 16, 1747, 3019
MhlI GDGCHC 1 cut(s) 3784
MlsI TGGCCA 1 cut(s) 4397
MluNI TGGCCA 1 cut(s) 4397
MlyI GAGTC 8 cut(s) 236, 1771, 2509, 2660, 2706, 3344, 3434, 4241
MmeI TCCRAC 5 cut(s) 514, 1222, 2828, 3073, 3652
Mox20I TGGCCA 1 cut(s) 4397
Mph1103I ATGCAT 1 cut(s) 1605
MroI TCCGGA 1 cut(s) 640
MroXI GAANNNNTTC 5 cut(s) 1197, 1220, 1635, 1944, 4064
MscI TGGCCA 1 cut(s) 4397
MslI CAYNNNNRTG 4 cut(s) 606, 2034, 3169, 3404
Msp20I TGGCCA 1 cut(s) 4397
MspA1I CMGCKG 1 cut(s) 1237
MspCI CTTAAG 2 cut(s) 179, 2440
MspI CCGG 4 cut(s) 54, 641, 2752, 3017
MspR9I CCNGG 8 cut(s) 1098, 1559, 1662, 2044, 3286, 3899, 4197, 4399
MunI CAATTG 6 cut(s) 195, 219, 1061, 2067, 2244, 2546
Mva1269I GAATGC 5 cut(s) 314, 1652, 1917, 3280, 3500
MvaI CCWGG 8 cut(s) 1098, 1559, 1662, 2044, 3286, 3899, 4197, 4399
MvnI CGCG 3 cut(s) 2966, 3778, 4351
MwoI GCNNNNNNNGC 7 cut(s) 302, 421, 2548, 2560, 2969, 3611, 3730
NcoI CCATGG 3 cut(s) 321, 3108, 3225
NlaIV GGNNCC 5 cut(s) 1797, 2041, 3021, 3324, 3884
NmeAIII GCCGAG 1 cut(s) 2339
NmuCI GTSAC 1 cut(s) 2659
NsiI ATGCAT 1 cut(s) 1605
NspI RCATGY 5 cut(s) 1470, 1735, 2591, 3728, 3943
NspV TTCGAA 1 cut(s) 3477
OliI CACNNNNGTG 2 cut(s) 3169, 3404
PaeI GCATGC 3 cut(s) 1735, 2591, 3943
PaeR7I CTCGAG 3 cut(s) 2510, 2859, 3422
PagI TCATGA 1 cut(s) 3840
PciSI GCTCTTC 2 cut(s) 1203, 1650
PcsI WCGNNNNNNNCGW 1 cut(s) 2372
PctI GAATGC 5 cut(s) 314, 1652, 1917, 3280, 3500
PdmI GAANNNNTTC 5 cut(s) 1197, 1220, 1635, 1944, 4064
Pfl23II CGTACG 1 cut(s) 66
PfoI TCCNGGA 2 cut(s) 1557, 3284
PinAI ACCGGT 1 cut(s) 2751
PkrI GCNGC 8 cut(s) 426, 743, 869, 2435, 2553, 2965, 3564, 4266
Ple19I CGATCG 1 cut(s) 391
PleI GAGTC 8 cut(s) 235, 1771, 2509, 2659, 2705, 3343, 3433, 4241
PpsI GAGTC 8 cut(s) 235, 1771, 2509, 2659, 2705, 3343, 3433, 4241
Ppu21I YACGTR 1 cut(s) 66
PshAI GACNNNNGTC 1 cut(s) 2666
PshBI ATTAAT 1 cut(s) 563
Psp6I CCWGG 8 cut(s) 1096, 1557, 1660, 2042, 3284, 3897, 4195, 4397
PspFI CCCAGC 1 cut(s) 3499
PspGI CCWGG 8 cut(s) 1096, 1557, 1660, 2042, 3284, 3897, 4195, 4397
PspLI CGTACG 1 cut(s) 66
PspN4I GGNNCC 5 cut(s) 1797, 2041, 3021, 3324, 3884
PspPI GGNCC 6 cut(s) 200, 1094, 2040, 2749, 3232, 3322
PspXI VCTCGAGB 1 cut(s) 3422
PsrI GAACNNNNNNTAC 2 cut(s) 59, 91
PstI CTGCAG 2 cut(s) 2537, 4099
PsuI RGATCY 3 cut(s) 16, 1747, 3019
PvuI CGATCG 1 cut(s) 391
PvuII CAGCTG 1 cut(s) 1237
RgaI GCGATCGC 1 cut(s) 391
RseI CAYNNNNRTG 4 cut(s) 606, 2034, 3169, 3404
SalI GTCGAC 1 cut(s) 1774
SapI GCTCTTC 2 cut(s) 1203, 1650
SatI GCNGC 8 cut(s) 425, 742, 868, 2434, 2552, 2964, 3563, 4265
Sau96I GGNCC 6 cut(s) 200, 1094, 2040, 2749, 3232, 3322
ScaI AGTACT 2 cut(s) 1015, 4204
SchI GAGTC 8 cut(s) 236, 1771, 2509, 2660, 2706, 3344, 3434, 4241
ScrFI CCNGG 8 cut(s) 1098, 1559, 1662, 2044, 3286, 3899, 4197, 4399
SduI GDGCHC 1 cut(s) 3784
SfaAI GCGATCGC 1 cut(s) 391
SfcI CTRYAG 4 cut(s) 1511, 2297, 2533, 4095
Sfr274I CTCGAG 3 cut(s) 2510, 2859, 3422
SfuI TTCGAA 1 cut(s) 3477
SgfI GCGATCGC 1 cut(s) 391
SinI GGWCC 5 cut(s) 200, 1094, 2040, 2749, 3232
SlaI CTCGAG 3 cut(s) 2510, 2859, 3422
SmiMI CAYNNNNRTG 4 cut(s) 606, 2034, 3169, 3404
SphI GCATGC 3 cut(s) 1735, 2591, 3943
SsiI CCGC 7 cut(s) 103, 132, 424, 681, 2964, 3776, 4349
SspI AATATT 3 cut(s) 541, 1953, 4189
SspMI CTAG 8 cut(s) 1301, 2102, 2282, 2330, 2381, 2712, 3254, 4419
StyD4I CCNGG 8 cut(s) 1096, 1557, 1660, 2042, 3284, 3897, 4195, 4397
StyI CCWWGG 6 cut(s) 321, 1436, 1935, 3108, 3225, 4084
TaiI ACGT 6 cut(s) 68, 1672, 1775, 1907, 4476, 4483
TatI WGTACW 7 cut(s) 967, 1013, 1705, 1829, 2600, 3622, 4202
TauI GCSGC 2 cut(s) 427, 2966
TseFI GTSAC 1 cut(s) 2659
TseI GCWGC 6 cut(s) 741, 867, 2433, 2551, 3562, 4264
Tsp45I GTSAC 1 cut(s) 2659
TspGWI ACGGA 5 cut(s) 2556, 2627, 2751, 2970, 3875
Vha464I CTTAAG 2 cut(s) 179, 2440
VpaK11BI GGWCC 5 cut(s) 200, 1094, 2040, 2749, 3232
VspI ATTAAT 1 cut(s) 563
XbaI TCTAGA 1 cut(s) 2711
XceI RCATGY 5 cut(s) 1470, 1735, 2591, 3728, 3943
XcmI CCANNNNNNNNNTGG 3 cut(s) 3223, 3360, 3507
XhoI CTCGAG 3 cut(s) 2510, 2859, 3422
XmiI GTMKAC 2 cut(s) 1775, 4456
XmnI GAANNNNTTC 5 cut(s) 1197, 1220, 1635, 1944, 4064
XspI CTAG 8 cut(s) 1301, 2102, 2282, 2330, 2381, 2712, 3254, 4419
ZraI GACGTC 1 cut(s) 1773
ZrmI AGTACT 2 cut(s) 1015, 4204
Zsp2I ATGCAT 1 cut(s) 1605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.