Rroxscaffold_3G00219690

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000129
Physical Location & Seq
Reverse (-)
1779532 .. 1798490
18959 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00219690.1

Sequence Viewer

Length: 4584 bp
ATGAAAGCCATGGGACAAAAAGTCCTCCCAGTGTTCTACGATGTTGATCCAAATGAGGTACGAAGACAAACAGGTGATCACTTTGGGAAAGCATTTGAGAAGCATCAAAAGCGGTTTAAAGCGGAACCAGACAAAGTAAAGAGGTGGAAAGACGCGCTTGTTCAAGTAGGCAATCTCTCCGGATGGCATCTGCAAGACGAGTACGAATCCAAAGTCATTCAAGAAATTGTTGAAAGTATTTTCACCGAGTTAAATCAATTAATCCCAATATCTGAGGGTTTAGTTGGGATGGATTCTCACCTGAATGAAATGCTTTCGTACTTAGACAATGGGTGTCCTGATGTTCGTATCATAGGGATTTGTGGGATGGGTGGTATCGGTAAGACAACTGTTGCACAAGTGGTTTTTGAAAGGGTACAAGCTCAGTTTGAAGGTTGCAGCTTTCTTGAGAATGTTAGAGAGGAAACAGAAAAGCAAGGTGCAATTCATTTACAAGAGCAACTTCTTTTGAACTTGCTGAAATGTAATGTAAATGTACAGACCACTAAAATGGGAAAAGAAATAATCAGGCATAGACTATGTACTAAAAGGGTTCTTCTCATTCTTGATGATGTGGATCAAGAAGAACAATTGGAAGCATTGTGTAATCGCACTTGGTTTGGTCCAGGGAGTAGGATCATCCTAACATCAAGAGATGAACAATTGCTCAGTTCATTTAGAGTGGATAAAGTATACAAGGTGAATCCATTAACTGCTGGCGAAGCTCTTGAGCTCTTTGGTATGAAAGCCTTTAAGAAAGACCAGCTGGTTGGGAAAGATTTTCTCAAGCTATCCAAGGAATTTTTGAAATATGCTAATGGTCTTCCATTAGCTATTAAAGTTTTGGGTTCCTCTGTTTATGGTAAGAATGTAAAATTATGGTCTAGTGCATTGGATAGACTAAAAAACAATCCTCAAAAAAAGATTATCAATGTTCTTAAAGCTAGTTATGATGGATTAGAAGAAACTGAAAAGAAAATATTTTTGGACATCGCATGTTTCTTTAAGGGATGGAACATAGCTCGTGTAACAAGTATACTGCAAGGTAGTGGCCACTGCCCGGACATTGATATAGAGGTTCTGGTACAGAAATCTTTGGTAACTCTGTTTGGAAGCGAATTGGGGATGCATGACTTGGTACAAGAACTGGGTTGGGATATTGTTCGTCAAGAAAGTCCTGAAGAGCCCGAAAAACGTAGCAGGTTGTGGCTTGCTGATGAAATCATCTATGTTCTTGGTCGGAGTAAGGCGATGAGTGCTGCTCAAAGTATATTCCTCCAATGTCCAACAAAAGACGATTTTGTCCACTCAATTGATAATGCCTTCTCAAATATGGACAGATTGAGATTGCTCAAGATTTGTAATGTGAAGTTTTTTGGAAACATCACATATCTTTCTAATGAGTTACAATATCTTGAGTGGCATGAGTGTCCTTTGGATTCTTTCCCGTCAGAATTTCAACCAAATAAACTTGTTGAAGTTTGCATGCAATTTAGCCGCATCAAACAACTATGGAGGGAGAAAAAAGGATGGAGCAAGCTACGGCTTATTGATCTGAGTGGTTCGCCATACTTGATGTCAACTCCAGACTTCACTGAGGTTCCTGATCTTCAGGAGTTGGAGCTTCAATATTGTACAAGCTTGGTGGAGGTTCACCCGTCTCTTGGATTTCTGAAGAAACTTGTCTCTCTGAATATGACACATTGCAGGTCTATTGAGAGCCTTCCACCTTTCACTGCCCTTGAATCCCTCCAAATATTGAGGCTTTCATTCTGTTCAGGGCTGAAGAAGTTTCCAGAAATTGAAGGAAATATGAAAAGCTTGCTGGAGCTTCATTTGGATAGGACCAGCATTGAGGAATTGCCTCCATCAATTGAACACTTGACTGGTCTTACCATATTGAATCTAACAGACTGTAAAAACCTTTTGCATCTTCCCGATACCGTTGGGTGCTTGACTTCTTTGAAAAGTCTCCATCTAACAGGTTGCTCCAAAATTGATGAGATACCTGAGAATCTGAAAGGTATGATATGTCTGGAGAAGCTTACAATTGGTGGAACTTCCATAAGGGAGTTATCTTTCATTGTAGGCATGAAGAATCTACGGTATCTATCCTGCGAAGGATGTAAATTTCTAGTTTCAGAATCATGCAAAGGCCTGGCTTCGATATCGAATTTGATAGTGCTAGACTTGAGTTATTGCAATCTGATGGATGGAGCAATTCTCAATGATTTTAGCAGCCTAATCTCCTTGGAATTTTTAGATTTAAGTGGTAATTGCTTCGTGCGATTACCTGAAAGTATCTCTCAACTCTCAAAGCTCGACACTTTGCACTTGAGTAACTGCAGACAGCTTCAATTGCTGCCCAAGAAGCTTCCGTTAAGTCTTCATCACGTTTATGCACAAGATTGTACTTCACTGACAGATTACCCAAATCAAATCAAAGAATTGAATTCATCGGAGTCGGGACTGACTATTATCGATTCCCTCAATTCTTCAGCACTCGGAGTCTCACAAAGTTCGTCCTGGTTGAATTTTTCAAGCATTGTAGGGGAACCTCTACAAGCGCATGTGACATATAATCATCCAGGGGGGATAAAAGTTTATCATGTGCCACGGATAACAGTTCTAAAACAAGAGCCTTTCTTTTCAGATTTCAGTAGCGCACATCCTACAGATGAAATTCCTGAGTGGTTCAGCGCTATATCTACCAGAAATCCCATATTAAGCCCGATACCTCAAAATCTGGCCGATGATAGCAAGTGGAAGGGATTTGCCGCGGGTGCTATATTCTCAGTCAAGGGGCACACTGCTCTCTCCCTTATTGAACCGGATCCTGATTTTTCTAATTACTCGTATCAAGTCACAATCGAAACTGATGTTGTGCGCCTGGAACCACAAGTGTTCGGGATAGGACTCAGCTGCCTTGCATCAAGTTCTCATCTGCTTATAATTTTCTATATAGAGTGTCTGAAGTTTCCACGATGGGGGTTAAATCAATCAACTGTGGTGAGAGCTATATTTGAAACCACCACTCCATCCATGGTGGTCCAGAAATGTGGAATTCATCTACTCTATGAGCAAGACGCTGGATGGCATTCCAGAATCCGTAGGCATCAACCGATTGTGCTGAATAAAAAGGACGCCGGCTCTCTACGGGATTCTGCCTGGCTTCGTCCATCATTTAGATGGGAGAAATTCATTCCACCCTTGGAAGAAGAATCCACACGTGTGCTGAGAAAGAACCTGGAGTCTGTTCTTCCAAGATACCTTGAGGCACTAAACTGTTATTCTAAAACGTACAAATTTAACCTCAGTGGAAGGCCAGGGTGGTTTCATGAGTCGTTTGTCTTTCCTCAGAGGGCTGGTTTTACTGTAGCATCAATCAAGCGGCCTCAAAATCTACACAAGAGCAAGAAGTGGATGGGATTTGCAATATATGCATCACTTGTAGAGGAAGTGGGGCAGACAATGGAAAAGGACAACTATTGGGTACGCGTATTTGTTACACCAAGAACTGGGGATAAATTTCGTTTGGGTGTGGTCCGAGTACTTAAGCACGTCAGGCCATTGTCAGAAGAACATCATCAACTATTGGTTATTTACATACCACGGGCACAGATTCCTGAAGTGTTGTTGACTCAGACATTAACAACTGCAATGGACTTTTACATCAAGATCAGTGATAGCGCTCATGTAAAGGTTCAAACATGTGGGTTCCGTATTGTGTACCAAGAAGATATACAAGGGTTTGCTGATACAATTATCCGGTGCATGCAAAGGGAAGATTCTCTTAAATCTCACAATAAGTTGGTGGACTGGTCTGACCCTCATCCTGTTTGTTGTCACTTCGAGGGAGCTGGAATTTTCAAGTTGAAATGGTTCATGCCTTTCATCAACCAAGGCAACTCTGCAGAAATCCAACTGCCTCTGAATGTGTTCAATGATGATAATTGGTTGGGCTTTGCCGTATGTTCTCAATTGTCCCACGACCAATATCCAAACATTAGTCTTGGCAGTACTGCTGCTGATTCAGAAGAGGTTATGCTTATTTGCTGTCTGGCCTCTGATGTTGCTTGGCAGGGCTTATTTGATTACAACTTTGAATTACGTTTAAAGATTCAAGAGCCATCGGAATACGATGCGACTTGGTTCTTTTATATACCACGCACTAAAGTTCGTAGTAATGTTTGGAGACAATGCAAATTGGCCAGGATAACTATGTGGTTGTCTAGTCCAAGCTCAGGAGTAGTGCAGAGTTGTGCCCTTCGTCTACTATTTAAGGAGGACGTTGAACATCTTCTTCGCAAGCCTGCATCGAATGAGAAGCTAATAACAAGTAATGCAATTGCCACCACATTTCCCTTGAATCAATCAGTTGTTAATATAAATTCAAAGCCAAAAGCTGAGGAGATAAATGAAAAACTTGAAGCATACGTCATGGAGATCCCTTCCAAGGCCCATTCTCCGGAACAAAGGCGGAAGACAGAGTGCCCTGAAACCAAGTCATCCTGGAGGAGTCATCAGTCTCCATGGCCATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1527

Amino Acids

173.43

Weight (kDa)

6.57

Isoelectric Point (pI)

47.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 2 - 90 2.8e-17 TIR domain
NB-ARC PF00931 99 - 267 2e-23 NB-ARC domain
NACHT PF05729 118 - 264 8.7e-06 NACHT domain
WHD_ROQ1 PF23282 334 - 405 3.7e-18 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 576 - 717 2.3e-12 Leucine-rich repeat region
LRR_13 PF23286 594 - 681 3.9e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 733 - 832 4.1e-09 Leucine-rich repeat region
C-JID PF20160 1307 - 1445 6.5e-06 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2990
AasI GACNNNNNNGTC 1 cut(s) 1340
Acc36I ACCTGC 2 cut(s) 1230, 1737
AccB7I CCANNNNNTGG 1 cut(s) 3557
AccI GTMKAC 3 cut(s) 732, 1075, 4312
AccII CGCG 3 cut(s) 155, 2819, 3537
AccIII TCCGGA 2 cut(s) 179, 4507
AciI CCGC 7 cut(s) 112, 122, 1537, 2817, 2819, 3430, 4519
AclWI GGATC 6 cut(s) 41, 624, 683, 2867, 2880, 4480
AcoI YGGCCR 4 cut(s) 1090, 2787, 4248, 4574
AcuI CTGAAG 7 cut(s) 1239, 1634, 1733, 1844, 2520, 3032, 3687
AcvI CACGTG 1 cut(s) 3269
AcyI GRCGYC 1 cut(s) 3183
AfeI AGCGCT 2 cut(s) 2740, 3730
AfiI CCNNNNNNNGG 7 cut(s) 1099, 1703, 3026, 3557, 4283, 4495, 4507
AflII CTTAAG 1 cut(s) 3593
AflIII ACRYGT 4 cut(s) 3266, 3268, 3535, 3749
AhdI GACNNNNNGTC 1 cut(s) 20
AjiI CACGTC 1 cut(s) 3601
AjuI GAANNNNNNNTTGG 2 cut(s) 1007, 1039
AleI CACNNNNGTG 1 cut(s) 3269
Alw21I GWGCWC 1 cut(s) 774
Alw26I GTCTC 6 cut(s) 1704, 1729, 2015, 2554, 4228, 4572
AlwI GGATC 6 cut(s) 41, 624, 683, 2867, 2880, 4480
Aor13HI TCCGGA 2 cut(s) 179, 4507
Aor51HI AGCGCT 2 cut(s) 2740, 3730
ApeKI GCWGC 6 cut(s) 438, 1298, 2277, 2401, 2961, 4064
ArsI GACNNNNNNTTYG 2 cut(s) 198, 230
AseI ATTAAT 1 cut(s) 260
Asp700I GAANNNNTTC 3 cut(s) 3920, 3977, 4338
AspLEI GCGC 6 cut(s) 157, 2608, 2705, 2741, 2928, 3731
AspS9I GGNCC 5 cut(s) 662, 1884, 3088, 3583, 4498
AsuC2I CCSGG 1 cut(s) 1100
AsuHPI GGTGA 6 cut(s) 86, 235, 290, 751, 1685, 3061
AvaII GGWCC 4 cut(s) 662, 1884, 3088, 3583
BaeGI GKGCMC 4 cut(s) 2847, 3658, 4306, 4535
BalI TGGCCA 3 cut(s) 1092, 4250, 4576
BamHI GGATCC 1 cut(s) 2872
BanII GRGCYC 2 cut(s) 774, 1227
BauI CACGAG 1 cut(s) 1062
BbrPI CACGTG 1 cut(s) 3269
BbsI GAAGAC 4 cut(s) 70, 854, 2417, 4529
Bbv12I GWGCWC 1 cut(s) 774
BbvCI CCTCAGC 1 cut(s) 4446
BbvI GCAGC 6 cut(s) 450, 1285, 2289, 2388, 2948, 4051
BceAI ACGGC 2 cut(s) 1598, 3992
BclI TGATCA 1 cut(s) 76
BcnI CCSGG 1 cut(s) 1100
BcoDI GTCTC 6 cut(s) 1704, 1729, 2015, 2554, 4228, 4572
BfaI CTAG 5 cut(s) 924, 984, 2174, 2225, 4272
BfmI CTRYAG 4 cut(s) 2383, 2712, 3414, 3951
BfoI RGCGCY 2 cut(s) 2742, 3732
BfrI CTTAAG 1 cut(s) 3593
BfuAI ACCTGC 2 cut(s) 1230, 1737
BisI GCNGC 9 cut(s) 439, 1299, 1537, 2278, 2402, 2817, 2962, 3431, 4065
BlsI GCNGC 9 cut(s) 440, 1300, 1538, 2279, 2403, 2818, 2963, 3432, 4066
BmcAI AGTACT 2 cut(s) 3591, 4060
Bme18I GGWCC 4 cut(s) 662, 1884, 3088, 3583
BmeRI GACNNNNNGTC 1 cut(s) 20
BmgBI CACGTC 1 cut(s) 3601
BmgT120I GGNCC 5 cut(s) 662, 1884, 3088, 3583, 4498
BmiI GGNNCC 7 cut(s) 126, 889, 1641, 2595, 2874, 2934, 3758
BmrI ACTGGG 3 cut(s) 23, 1196, 3567
BmuI ACTGGG 3 cut(s) 23, 1196, 3567
BpiI GAAGAC 4 cut(s) 70, 854, 2417, 4529
BplI GAGNNNNNCTC 4 cut(s) 1285, 1317, 2247, 2279
BpmI CTGGAG 5 cut(s) 1608, 1886, 2096, 3308, 4573
Bpu10I CCTNAGC 2 cut(s) 4282, 4446
BpuEI CTTGAG 8 cut(s) 467, 788, 809, 1376, 1475, 2251, 2395, 3332
BpuMI CCSGG 1 cut(s) 1100
Bsa29I ATCGAT 1 cut(s) 2520
BsaAI YACGTR 1 cut(s) 3269
BsaBI GATNNNNATC 2 cut(s) 45, 615
BsaHI GRCGYC 1 cut(s) 3183
BsaWI WCCGGW 4 cut(s) 179, 2869, 3807, 4507
BsaXI ACNNNNNCTCC 2 cut(s) 3058, 3088
Bsc4I CCNNNNNNNGG 7 cut(s) 1099, 1703, 3026, 3557, 4283, 4495, 4507
Bse118I RCCGGY 1 cut(s) 3185
Bse1I ACTGG 5 cut(s) 29, 1191, 1930, 3562, 3863
Bse3DI GCAATG 2 cut(s) 1741, 3705
Bse8I GATNNNNATC 2 cut(s) 45, 615
BseAI TCCGGA 2 cut(s) 179, 4507
BseCI ATCGAT 1 cut(s) 2520
BseJI GATNNNNATC 2 cut(s) 45, 615
BseLI CCNNNNNNNGG 7 cut(s) 1099, 1703, 3026, 3557, 4283, 4495, 4507
BseMI GCAATG 2 cut(s) 1741, 3705
BseNI ACTGG 5 cut(s) 29, 1191, 1930, 3562, 3863
BseRI GAGGAG 2 cut(s) 4463, 4570
BseSI GKGCMC 4 cut(s) 2847, 3658, 4306, 4535
BseXI GCAGC 6 cut(s) 450, 1285, 2289, 2388, 2948, 4051
BsgI GTGCAG 1 cut(s) 4313
Bsh1236I CGCG 3 cut(s) 155, 2819, 3537
BshVI ATCGAT 1 cut(s) 2520
BsiHKAI GWGCWC 1 cut(s) 774
BsiSI CCGG 6 cut(s) 180, 1100, 2870, 3186, 3808, 4508
BslFI GGGAC 3 cut(s) 27, 2520, 4009
BslI CCNNNNNNNGG 7 cut(s) 1099, 1703, 3026, 3557, 4283, 4495, 4507
BsmAI GTCTC 6 cut(s) 1704, 1729, 2015, 2554, 4228, 4572
BsmBI CGTCTC 1 cut(s) 1704
BsmFI GGGAC 3 cut(s) 27, 2520, 4009
BsmI GAATGC 1 cut(s) 3136
Bsp1286I GDGCHC 6 cut(s) 774, 1227, 2847, 3658, 4306, 4535
Bsp13I TCCGGA 2 cut(s) 179, 4507
Bsp1407I TGTACA 2 cut(s) 535, 1673
Bsp143I GATC 9 cut(s) 46, 76, 616, 675, 1591, 1645, 2872, 3717, 4485
Bsp19I CCATGG 3 cut(s) 9, 3081, 4571
BspACI CCGC 7 cut(s) 112, 122, 1537, 2817, 2819, 3430, 4519
BspDI ATCGAT 1 cut(s) 2520
BspEI TCCGGA 2 cut(s) 179, 4507
BspFNI CGCG 3 cut(s) 155, 2819, 3537
BspHI TCATGA 1 cut(s) 3376
BspLI GGNNCC 7 cut(s) 126, 889, 1641, 2595, 2874, 2934, 3758
BspMAI CTGCAG 2 cut(s) 2387, 3955
BspMI ACCTGC 2 cut(s) 1230, 1737
BspPI GGATC 6 cut(s) 41, 624, 683, 2867, 2880, 4480
BspQI GCTCTTC 1 cut(s) 1215
BspTI CTTAAG 1 cut(s) 3593
BsrDI GCAATG 2 cut(s) 1741, 3705
BsrFI RCCGGY 1 cut(s) 3185
BsrGI TGTACA 2 cut(s) 535, 1673
BsrI ACTGG 5 cut(s) 29, 1191, 1930, 3562, 3863
BssAI RCCGGY 1 cut(s) 3185
BssMI GATC 9 cut(s) 46, 76, 616, 675, 1591, 1645, 2872, 3717, 4485
BssNAI GTATAC 2 cut(s) 733, 1076
BssNI GRCGYC 1 cut(s) 3183
BssSI CACGAG 1 cut(s) 1062
BssT1I CCWWGG 8 cut(s) 9, 834, 2289, 3081, 3249, 3940, 4494, 4571
Bst1107I GTATAC 2 cut(s) 733, 1076
Bst2BI CACGAG 1 cut(s) 1062
Bst4CI ACNGT 8 cut(s) 391, 1955, 1984, 2145, 2665, 3046, 3326, 3415
Bst6I CTCTTC 2 cut(s) 1215, 4071
BstACI GRCGYC 1 cut(s) 3183
BstAFI CTTAAG 1 cut(s) 3593
BstAPI GCANNNNNTGC 1 cut(s) 3479
BstAUI TGTACA 2 cut(s) 535, 1673
BstBAI YACGTR 1 cut(s) 3269
BstC8I GCNNGC 9 cut(s) 757, 1251, 1526, 1577, 1862, 3187, 3815, 4349, 4353
BstDSI CCRYGG 6 cut(s) 9, 2654, 2817, 3081, 3650, 4571
BstFNI CGCG 3 cut(s) 155, 2819, 3537
BstH2I RGCGCY 2 cut(s) 2742, 3732
BstHHI GCGC 6 cut(s) 157, 2608, 2705, 2741, 2928, 3731
BstKTI GATC 9 cut(s) 49, 79, 619, 678, 1594, 1648, 2875, 3720, 4488
BstMAI GTCTC 6 cut(s) 1704, 1729, 2015, 2554, 4228, 4572
BstMBI GATC 9 cut(s) 46, 76, 616, 675, 1591, 1645, 2872, 3717, 4485
BstMWI GCNNNNNNNGC 8 cut(s) 109, 761, 1295, 2398, 2410, 2822, 3479, 3604
BstNSI RCATGY 5 cut(s) 1038, 1528, 2612, 3753, 3817
BstSFI CTRYAG 4 cut(s) 2383, 2712, 3414, 3951
BstSLI GKGCMC 4 cut(s) 2847, 3658, 4306, 4535
BstUI CGCG 3 cut(s) 155, 2819, 3537
BstV1I GCAGC 6 cut(s) 450, 1285, 2289, 2388, 2948, 4051
BstV2I GAAGAC 4 cut(s) 70, 854, 2417, 4529
BstX2I RGATCY 2 cut(s) 2872, 4485
BstXI CCANNNNNNTGG 3 cut(s) 550, 809, 3098
BstYI RGATCY 2 cut(s) 2872, 4485
BstZ17I GTATAC 2 cut(s) 733, 1076
Bsu15I ATCGAT 1 cut(s) 2520
BsuTUI ATCGAT 1 cut(s) 2520
BtgI CCRYGG 6 cut(s) 9, 2654, 2817, 3081, 3650, 4571
BtgZI GCGATG 2 cut(s) 1015, 1304
BtrI CACGTC 1 cut(s) 3601
BtsI GCAGTG 3 cut(s) 1093, 1773, 2847
BtsIMutI CAGTG 8 cut(s) 36, 1093, 1632, 1773, 2456, 2847, 3361, 3727
BveI ACCTGC 2 cut(s) 1230, 1737
Cac8I GCNNGC 9 cut(s) 757, 1251, 1526, 1577, 1862, 3187, 3815, 4349, 4353
CciI TCATGA 1 cut(s) 3376
CfoI GCGC 6 cut(s) 157, 2608, 2705, 2741, 2928, 3731
Cfr10I RCCGGY 1 cut(s) 3185
Cfr13I GGNCC 5 cut(s) 662, 1884, 3088, 3583, 4498
Cfr42I CCGCGG 1 cut(s) 2820
ClaI ATCGAT 1 cut(s) 2520
CseI GACGC 3 cut(s) 161, 3134, 3191
CspCI CAANNNNNGTGG 2 cut(s) 1665, 1700
DpnI GATC 9 cut(s) 48, 78, 618, 677, 1593, 1647, 2874, 3719, 4487
DpnII GATC 9 cut(s) 46, 76, 616, 675, 1591, 1645, 2872, 3717, 4485
DraI TTTAAA 2 cut(s) 118, 4155
DrdI GACNNNNNNGTC 1 cut(s) 1340
DriI GACNNNNNGTC 1 cut(s) 20
DseDI GACNNNNNNGTC 1 cut(s) 1340
EaeI YGGCCR 4 cut(s) 1090, 2787, 4248, 4574
Eam1104I CTCTTC 2 cut(s) 1215, 4071
Eam1105I GACNNNNNGTC 1 cut(s) 20
EarI CTCTTC 2 cut(s) 1215, 4071
EciI GGCGGA 1 cut(s) 4534
Ecl136II GAGCTC 1 cut(s) 772
Eco130I CCWWGG 8 cut(s) 9, 834, 2289, 3081, 3249, 3940, 4494, 4571
Eco147I AGGCCT 1 cut(s) 2196
Eco24I GRGCYC 2 cut(s) 774, 1227
Eco32I GATATC 1 cut(s) 2208
Eco47I GGWCC 4 cut(s) 662, 1884, 3088, 3583
Eco47III AGCGCT 2 cut(s) 2740, 3730
Eco53kI GAGCTC 1 cut(s) 772
Eco57I CTGAAG 7 cut(s) 1239, 1634, 1733, 1844, 2520, 3032, 3687
Eco72I CACGTG 1 cut(s) 3269
EcoICRI GAGCTC 1 cut(s) 772
EcoRI GAATTC 2 cut(s) 2491, 3102
EcoRV GATATC 1 cut(s) 2208
EcoT14I CCWWGG 8 cut(s) 9, 834, 2289, 3081, 3249, 3940, 4494, 4571
EcoT22I ATGCAT 2 cut(s) 1170, 3484
EcoT38I GRGCYC 2 cut(s) 774, 1227
ErhI CCWWGG 8 cut(s) 9, 834, 2289, 3081, 3249, 3940, 4494, 4571
Esp3I CGTCTC 1 cut(s) 1704
FalI AAGNNNNNCTT 6 cut(s) 141, 173, 486, 518, 3816, 3848
FaqI GGGAC 3 cut(s) 27, 2520, 4009
FauI CCCGC 1 cut(s) 2812
FbaI TGATCA 1 cut(s) 76
FblI GTMKAC 3 cut(s) 732, 1075, 4312
Fnu4HI GCNGC 9 cut(s) 439, 1299, 1537, 2278, 2402, 2817, 2962, 3431, 4065
FriOI GRGCYC 2 cut(s) 774, 1227
Fsp4HI GCNGC 9 cut(s) 439, 1299, 1537, 2278, 2402, 2817, 2962, 3431, 4065
FspBI CTAG 5 cut(s) 924, 984, 2174, 2225, 4272
GlaI GCGC 6 cut(s) 156, 2607, 2704, 2740, 2927, 3730
GluI GCNGC 9 cut(s) 439, 1299, 1537, 2278, 2402, 2817, 2962, 3431, 4065
GsuI CTGGAG 5 cut(s) 1608, 1886, 2096, 3308, 4573
HaeII RGCGCY 2 cut(s) 2742, 3732
HapII CCGG 6 cut(s) 180, 1100, 2870, 3186, 3808, 4508
HgaI GACGC 3 cut(s) 161, 3134, 3191
HhaI GCGC 6 cut(s) 157, 2608, 2705, 2741, 2928, 3731
Hin1I GRCGYC 1 cut(s) 3183
Hin6I GCGC 6 cut(s) 155, 2606, 2703, 2739, 2926, 3729
HinP1I GCGC 6 cut(s) 155, 2606, 2703, 2739, 2926, 3729
HincII GTYRAC 2 cut(s) 1620, 3678
HindII GTYRAC 2 cut(s) 1620, 3678
HindIII AAGCTT 4 cut(s) 1678, 1858, 2081, 2411
HpaII CCGG 6 cut(s) 180, 1100, 2870, 3186, 3808, 4508
HphI GGTGA 6 cut(s) 86, 235, 290, 751, 1685, 3061
Hpy166II GTNNAC 9 cut(s) 733, 1076, 1345, 1620, 1693, 3678, 3769, 3856, 4313
Hpy8I GTNNAC 9 cut(s) 733, 1076, 1345, 1620, 1693, 3678, 3769, 3856, 4313
HpyAV CCTTC 9 cut(s) 425, 1372, 1772, 1838, 2153, 2800, 3354, 4316, 4500
HpyCH4III ACNGT 8 cut(s) 391, 1955, 1984, 2145, 2665, 3046, 3326, 3415
HpyCH4IV ACGT 8 cut(s) 1234, 2433, 3268, 3338, 3600, 4150, 4329, 4476
HpyF10VI GCNNNNNNNGC 8 cut(s) 109, 761, 1295, 2398, 2410, 2822, 3479, 3604
HpySE526I ACGT 8 cut(s) 1234, 2433, 3268, 3338, 3600, 4150, 4329, 4476
Hsp92I GRCGYC 1 cut(s) 3183
HspAI GCGC 6 cut(s) 155, 2606, 2703, 2739, 2926, 3729
Kpn2I TCCGGA 2 cut(s) 179, 4507
KroI GCCGGC 1 cut(s) 3185
KroNI GCCGGC 1 cut(s) 3187
Ksp22I TGATCA 1 cut(s) 76
KspI CCGCGG 1 cut(s) 2820
Kzo9I GATC 9 cut(s) 46, 76, 616, 675, 1591, 1645, 2872, 3717, 4485
LguI GCTCTTC 1 cut(s) 1215
LmnI GCTCC 6 cut(s) 1572, 1660, 1867, 2033, 2255, 3896
Lsp1109I GCAGC 6 cut(s) 450, 1285, 2289, 2388, 2948, 4051
MaeI CTAG 5 cut(s) 924, 984, 2174, 2225, 4272
MaeII ACGT 8 cut(s) 1234, 2433, 3268, 3338, 3600, 4150, 4329, 4476
MaeIII GTNAC 8 cut(s) 1066, 1138, 1443, 2378, 2611, 2902, 3544, 3884
MalI GATC 9 cut(s) 48, 78, 618, 677, 1593, 1647, 2874, 3719, 4487
MboI GATC 9 cut(s) 46, 76, 616, 675, 1591, 1645, 2872, 3717, 4485
MfeI CAATTG 8 cut(s) 629, 701, 1350, 1911, 2088, 2396, 4019, 4386
MflI RGATCY 2 cut(s) 2872, 4485
MhlI GDGCHC 6 cut(s) 774, 1227, 2847, 3658, 4306, 4535
MlsI TGGCCA 3 cut(s) 1092, 4250, 4576
MluI ACGCGT 1 cut(s) 3535
MluNI TGGCCA 3 cut(s) 1092, 4250, 4576
MlyI GAGTC 7 cut(s) 2510, 2556, 2949, 3299, 3389, 3673, 4567
MmeI TCCRAC 4 cut(s) 1259, 1349, 1638, 3985
Mox20I TGGCCA 3 cut(s) 1092, 4250, 4576
Mph1103I ATGCAT 2 cut(s) 1170, 3484
MroI TCCGGA 2 cut(s) 179, 4507
MroNI GCCGGC 1 cut(s) 3185
MroXI GAANNNNTTC 3 cut(s) 3920, 3977, 4338
MscI TGGCCA 3 cut(s) 1092, 4250, 4576
MslI CAYNNNNRTG 5 cut(s) 303, 548, 2436, 3226, 3269
Msp20I TGGCCA 3 cut(s) 1092, 4250, 4576
MspA1I CMGCKG 3 cut(s) 805, 2819, 2961
MspCI CTTAAG 1 cut(s) 3593
MspI CCGG 6 cut(s) 180, 1100, 2870, 3186, 3808, 4508
MunI CAATTG 8 cut(s) 629, 701, 1350, 1911, 2088, 2396, 4019, 4386
Mva1269I GAATGC 1 cut(s) 3136
MvnI CGCG 3 cut(s) 155, 2819, 3537
MwoI GCNNNNNNNGC 8 cut(s) 109, 761, 1295, 2398, 2410, 2822, 3479, 3604
NaeI GCCGGC 1 cut(s) 3187
NciI CCSGG 1 cut(s) 1100
NcoI CCATGG 3 cut(s) 9, 3081, 4571
NdeII GATC 9 cut(s) 46, 76, 616, 675, 1591, 1645, 2872, 3717, 4485
NgoMIV GCCGGC 1 cut(s) 3185
NlaIV GGNNCC 7 cut(s) 126, 889, 1641, 2595, 2874, 2934, 3758
NmuCI GTSAC 3 cut(s) 2611, 2902, 3884
NsiI ATGCAT 2 cut(s) 1170, 3484
NspI RCATGY 5 cut(s) 1038, 1528, 2612, 3753, 3817
OliI CACNNNNGTG 1 cut(s) 3269
PaeI GCATGC 2 cut(s) 1528, 3817
PagI TCATGA 1 cut(s) 3376
PceI AGGCCT 1 cut(s) 2196
PciI ACATGT 1 cut(s) 3749
PciSI GCTCTTC 1 cut(s) 1215
PctI GAATGC 1 cut(s) 3136
PdiI GCCGGC 1 cut(s) 3187
PdmI GAANNNNTTC 3 cut(s) 3920, 3977, 4338
PflMI CCANNNNNTGG 1 cut(s) 3557
PfoI TCCNGGA 1 cut(s) 4550
PkrI GCNGC 9 cut(s) 440, 1300, 1538, 2279, 2403, 2818, 2963, 3432, 4066
PleI GAGTC 7 cut(s) 2509, 2555, 2949, 3298, 3388, 3673, 4566
PmaCI CACGTG 1 cut(s) 3269
PmlI CACGTG 1 cut(s) 3269
PpsI GAGTC 7 cut(s) 2509, 2555, 2949, 3298, 3388, 3673, 4566
Ppu21I YACGTR 1 cut(s) 3269
PscI ACATGT 1 cut(s) 3749
PshBI ATTAAT 1 cut(s) 260
PsiI TTATAA 1 cut(s) 2990
Psp124BI GAGCTC 1 cut(s) 774
PspCI CACGTG 1 cut(s) 3269
PspN4I GGNNCC 7 cut(s) 126, 889, 1641, 2595, 2874, 2934, 3758
PspPI GGNCC 5 cut(s) 662, 1884, 3088, 3583, 4498
PstI CTGCAG 2 cut(s) 2387, 3955
PsuI RGATCY 2 cut(s) 2872, 4485
PvuII CAGCTG 2 cut(s) 805, 2961
RseI CAYNNNNRTG 5 cut(s) 303, 548, 2436, 3226, 3269
SacI GAGCTC 1 cut(s) 774
SacII CCGCGG 1 cut(s) 2820
SapI GCTCTTC 1 cut(s) 1215
SatI GCNGC 9 cut(s) 439, 1299, 1537, 2278, 2402, 2817, 2962, 3431, 4065
Sau3AI GATC 9 cut(s) 46, 76, 616, 675, 1591, 1645, 2872, 3717, 4485
Sau96I GGNCC 5 cut(s) 662, 1884, 3088, 3583, 4498
ScaI AGTACT 2 cut(s) 3591, 4060
SchI GAGTC 7 cut(s) 2510, 2556, 2949, 3299, 3389, 3673, 4567
SduI GDGCHC 6 cut(s) 774, 1227, 2847, 3658, 4306, 4535
SfcI CTRYAG 4 cut(s) 2383, 2712, 3414, 3951
Sfr303I CCGCGG 1 cut(s) 2820
SgrBI CCGCGG 1 cut(s) 2820
SinI GGWCC 4 cut(s) 662, 1884, 3088, 3583
SmiMI CAYNNNNRTG 5 cut(s) 303, 548, 2436, 3226, 3269
SmlI CTYRAG 9 cut(s) 446, 767, 824, 1391, 1454, 2230, 2374, 3311, 3593
SmoI CTYRAG 9 cut(s) 446, 767, 824, 1391, 1454, 2230, 2374, 3311, 3593
SphI GCATGC 2 cut(s) 1528, 3817
SseBI AGGCCT 1 cut(s) 2196
SsiI CCGC 7 cut(s) 112, 122, 1537, 2817, 2819, 3430, 4519
SspI AATATT 3 cut(s) 1020, 1670, 1797
SspMI CTAG 5 cut(s) 924, 984, 2174, 2225, 4272
SstI GAGCTC 1 cut(s) 774
StuI AGGCCT 1 cut(s) 2196
StyI CCWWGG 8 cut(s) 9, 834, 2289, 3081, 3249, 3940, 4494, 4571
TaaI ACNGT 8 cut(s) 391, 1955, 1984, 2145, 2665, 3046, 3326, 3415
TaiI ACGT 8 cut(s) 1237, 2436, 3271, 3341, 3603, 4153, 4332, 4479
TaqI TCGA 7 cut(s) 2204, 2210, 2361, 2520, 2910, 3891, 4358
TatI WGTACW 6 cut(s) 535, 581, 1673, 2450, 3589, 4058
TauI GCSGC 3 cut(s) 1539, 2819, 3433
TscAI CASTG 8 cut(s) 36, 1100, 1639, 1780, 2463, 2854, 3361, 3727
TseFI GTSAC 3 cut(s) 2611, 2902, 3884
TseI GCWGC 6 cut(s) 438, 1298, 2277, 2401, 2961, 4064
Tsp45I GTSAC 3 cut(s) 2611, 2902, 3884
TspGWI ACGGA 4 cut(s) 2406, 2671, 3137, 3749
TspRI CASTG 8 cut(s) 36, 1100, 1639, 1780, 2463, 2854, 3361, 3727
Van91I CCANNNNNTGG 1 cut(s) 3557
Vha464I CTTAAG 1 cut(s) 3593
VpaK11BI GGWCC 4 cut(s) 662, 1884, 3088, 3583
VspI ATTAAT 1 cut(s) 260
XceI RCATGY 5 cut(s) 1038, 1528, 2612, 3753, 3817
XcmI CCANNNNNNNNNTGG 2 cut(s) 3079, 3225
XmiI GTMKAC 3 cut(s) 732, 1075, 4312
XmnI GAANNNNTTC 3 cut(s) 3920, 3977, 4338
XspI CTAG 5 cut(s) 924, 984, 2174, 2225, 4272
ZrmI AGTACT 2 cut(s) 3591, 4060
Zsp2I ATGCAT 2 cut(s) 1170, 3484
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.