FvH4_5g38540

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000129
Physical Location & Seq
Forward (+)
28487139 .. 28494454
7316 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g38540.t3

Sequence Viewer

Length: 4815 bp
ATGATTCCTCCTCCTCCTTCTTCGTCTTCTTATCCACCATCCGGTAAATTCACGTACGATGTGTTCCTCAGTTTCAGAGGTACAGATACCCGCACAAACTTCACGGACCACCTTTATACTGCTCTCATACAAAAAGGCATATTCACATTTAGGGATGATGAAGAACTAAAGAGTGGGGAATCGATTGGTCCAAATCTGTTGAAAGCAATAGAAGAATCAAGATATGTAATTGTTGTTTTCTCACCAAACTACGCCGAATCTGCCTGGTGTTTGGATGAGATTGTCAAGGTCAACGATTGCAGGAAAGCTATGGGACAAGTAGTCCTTCCAGTGTTCTACCATTGTGATCCATCTGAGGTACGAAGACAGACAGGGGAGCAATTCGGGAAAGCATTTGAGAAGCATCAAGAGCGGTATATAGCGGAACCAGACAAAGTGAAGAGGTGGAAAGAGGCTCTGTTTGAAGTTGGCAGTCTCTCTGGATGGCATCTGCAAGATGGGTACGAATCCAAAGTCATTCAAGCAATTGTTGATAAGACTTTCACCGAGTTAAATCAATTAATTTCGAAATATGAGGATTTAGTTGGGATGGATTCTCACCTGAATGGAATGCTTTCATGCTTAGACATTGGGTGTCCTGATGTTCGTATCATAGGGATTTGTGGGATGGGTGGTATCGGAAAGACCACTATTGCACAGGTGGTTTTTAAAAGGCTACGAGCCCAGTTTGATGGTTGCAGCTTTCTTGAGAATGTTAGAGAGGTGATTATTAAAAAGCAAGGTGCAGTTTGTTTACAACAGCAACTTCTTTCCGATTTGCTGAAAGGTGATGTTAGTGTACAGAACAGTGGCAGAGGAAAAGATATAATTAGCCATAGACTGCGTTCTAAAAGGGTTCTTATCATTCTCGATGATGTGGACAAAGAAAAACAATTGGAAATATTGTGTGCACGTGATCGGGAATCGTTTGGTCCAGGGAGTAGAATCATCATAACTTGTAGAGATGAACGTTTGGTCAGGGAAGATGAAGGAGATAAACTATATAGGGTGAATCCATTAACTGATGCTGAAGCACTTCAGTTTTTTAGTATGAAAGCCTACAAGAAAGACGAGCTGGTTGGAGATGAAAGTCCCAAGCAATCCGATGAATTTTCTCAGAAAAAAAAGCTATCCGAGGAGTTTCTGAAATATGCTAATGGCCATCCATTAACTATCAAAGTTTTGGGTGGGTCTGTTCGTGGTAGAAGTGTAAAATTATGGTCAAGCACGTTACAAAGATTAGAAGAAAATCCTGAAAAAGAAATTATTGATGTTCTTAAGGTTAGTTTCAATGGATTGCGAGAAACAGAGAAGAAAACTTTTTTAGACATCGCATGTTTCTTTAAAGGAGAGGACAAAGATCGTGTTATAAGAATCCTAGAAGGCCACGGCTCATTTCCGGATATTGATATAGAAGTTCTGATAGAGAAATCTATGGTAACTATGTCTAGAAATAAATTGGGGATGCATGATTTGATACAAGATTTGGGTCGGGAGATTGTGCGTCAAGAATGTCCTGAAGAGCCTGGAAAACGTAGCAGGTTGTGGATTCCCAAGGACATCATCCGTGTACTTAAGCGGAGTAAGGCAACGGATGCAGTCCAAAGCATATACCTCCAATGTGCAAAAAAAAACGATGTCGTTCACTCAATTAATGATGCCTTTTCAGTTATGGACAGATTAAGATTGCTCAAGATTGATAATTTGAAATTTTCCGGAAACATCTCATATCTTTCTAATGAGTTACAGTATCTTGAATGGCATGAATGTCCTTTCGATTCCTTCCCATCAGACTTTCAACCAGACAAACTCGTTGAAGTTCACATGTATTTTAGCCGCATCAAACAACTTTGGGGAGGAAAAAAAGGATGGAGCATGCTACGGCGTTTAGATCTGAGTGGTTCACCATTCTTGATGTCAACTCCAGACTTCACTGAAGTTCCTGATCTAGAAGTGTTGGCACTTCAAGATTGCACAAGCTTGGTGGAGGTTCACCCGTCTCTTGGATTGCTCAAGAAACTTAGCTTTTTGGATATGAAAAATTGCAAGTCTATTGAGAGCCTTCCACCTTTCACTACCTTGGAATCCCTTCAAAAATTGAGGCTTTCTCTCTGCTCAAAACTGAAGAAGTTTCCAGAAATTGATGGGAATATGAAAAGCTTGCTGGAGCTTCATTTGGATGGGACCAGCATTGAGGAATTGCAGCCATCAATTGAACGCTTCACTGGTCTCTTAATGATGAATCTAACAGACTGTAAAAACCTTTTGCGTCTTCCCAGTAACATTGGGTGCTTGACATCTTTGAAAAGTCTCTCTCTAACAGGTTGCTCCAAAATTGATGAGATCCCCGAGAATCTGAATGGTATGAAATGTCTGGAGCAGCTTTTCATTGGCAGAACTTCCATAAGGGAGTTATCTTTCATTGCAGGCATGAAGAATCTACAGTATCTATCCTGCCAAGGATGTACATGTCTAATTTCAGAATCATGCAAAGGTCTGGCTTCATTATCGAGTTTGATATCGCTAGACTTGAGTTATTGCAATCTGATGGATGGAGCGTTTCTCAATTATTTTAGCAGCCTAATCTCCTTAAGATGTTTAGGCTTAAGTGGTAATTGCTTTGTGCGATTACCTGAAAGTATCTCTCAACTCTCAAAGCTTGAGATTCTGGACTTGAGTAATTGTAGACAGCTTCTATTGCTGCCCAAGAACCTTCCGTTTAGTCTTCAAACCGTGTATGCACAAGACTGTACTTCACTGAAGGACTACCCAAACCAAATCAAAGTATTGAAATCATTCAATTCAGGAGTGACTATTGTCAATTCACTCAATTCTTCAGCTCCTGGAATCTCACAAAGTTCCACAAGGACCTCAACGTTGTCTTTCTCAGGAGGTGAGGGGGAAGATACTGCGATATATCAGGATGGAGAGGGAAAACAAGTTCATCATTTGCCACAATTGCAGAGGCCGCCTCCCTTCTCAATTTCGAATTCCAGTTATGTACTTCCTACAGATGAAATTCCAGACTGGTTCACCATATCTACCAGAAATCCCATTTCAATTCTGATGCCTCAAAATGTGGCCAATGATAACAAGTGGAAGGGATTTGCCGTGTGTGCTATATTCGCAGTCAAGGGGCATACAGCTCTATCCGTTATTGAACCGGATCCTGATTTCTTTAATTACTTGTATCAAGTCACATTGGAAACTGACGTTATGCGTTTTGAACCGGTAGTGTTTGATTTCACTGGCCTAGATCGCAACCTTGGATCATGTTCTCATCTGCTTGTGATTTTTAATATATCTAGATTCACGTTTCCGTCGTGGTGGTTAAATGATTCAACTGAGGTGAGGGCTATATTTGAAACCACCAATCCATCCATGGTGTTTCAGAAATGCGGGGTTCGTCTAGTGTATGAGCAAGATGTTGCATTGTATTCCAGAATATTCAACGAGGATCAAGCGATTTGTCTGGAAAAGGTTGAGGACGAGACCTCTGTACCGGACTCTGCCTGGCTTAATCAATCTTTGAAATGGGAGAAATTTATTCCACCTTCGGAAGAAGAATCCACACTCGTGGCGAGAAAGAACCTTGAGTCTGTTCTTCCAAGATACCTTGAGGCACTAAACAGTTATCATGAATCCTACAAATTTAACCTCAGAGGAAGTCCAGGGTGGTTTGACCAGTCGTTTATCCCTGCTCATACGAGGTCAAATATGAGCAGTGTGTTAATCGAGCTGCCTCAAAATCTACAGAAGAGCAGGAAGTGGATGGGATTTGCAATATACGCATCACTTGTAGAGGAAGTGGGGCGGACGATGAAAACAGCCGACTATGGGGTATCTGTTAATCTAGAGTATGCGAATATATTATATTCGGTTGTTATTCCAGGACTTAAGCACGTCAGGCCATTGTCAGAAGAACATCATCAATTATTGGTTATTTACATACCGCGGGCAAAGATTCTCGAAGTGTTGTCCACTCAGACATTCACAATTGTTGCAATGGACTTTATCAGTGATAGCCCTCATGTAAAGGTTCAAAGATATGGTTTCCGTATTGTGTACCAGGAAGATATACAAGGGTTTGCTGATACGATTATCAGGTGTATGCAGAGGGATGATTCTCTTAAATCTTACGATAAGTTGGTAGTAGAGAAATGGATGCAGTTGCTACGATTGCAAGGTGCCCGTTTAGAGAGAATGACCAAACGAGATTCCAGAACCGCACAAGCAACCTTCAGAAATTGGGACTGGTCTGACCCTCGTTCTGCTTATTGTTTGTTCGATAGAGTCTCGAAGTTGAAATGGTTCGTGCCTTTCATCAACCAAGGAAACTCTGCCGAAATCCAACTGCCTCCGAATGTGTTTGATAATCATAATTGGTTGGGGTTTGCTGTATGTGTTCAGTGCTCCCGCTCTTCTTCTTCTTCAGAGGATGTTGTGCTTGCTTATCGACTGGACTCTGATGTTGCTTGGCACGGCTTATTTGATTACAAGATGCGAGCAGTTATGAAGCTACCCGAGACACCTAAAGACGAATTATATGATGCGACTATGTTCGTTTATATACCGCGCACTAAAGCTCTTATGAGTAAGATGTGGAGGCAATGCAAATTGGCCAGGATAACTGTGTGGTTCTCCAACCCATGTGCAGCAATAGTGCATACTTGTGCCCTTCGTCTACTATTTAAGGACGACATTGAACATCTTGTTGAGACACTCACTGTTGCATCTGACGAAAACCGTTATTTCCTCGGTCTCACTGCTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1605

Amino Acids

182.44

Weight (kDa)

6.19

Isoelectric Point (pI)

47.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 19 - 190 9e-51 TIR domain
TIR_2 PF13676 21 - 118 3.1e-15 TIR domain
NB-ARC PF00931 213 - 370 2.1e-21 NB-ARC domain
WHD_ROQ1 PF23282 448 - 516 4.3e-18 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 737 - 824 9.7e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 776 - 882 3.2e-08 Leucine-rich repeat region
LRR_14 PF23598 877 - 975 2.4e-08 Leucine-rich repeat region
C-JID PF20160 1045 - 1186 8.8e-09 C-JID domain
C-JID PF20160 1484 - 1612 2.5e-06 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1409
Acc36I ACCTGC 1 cut(s) 1569
AccB1I GGYRCC 1 cut(s) 4226
AccBSI CCGCTC 2 cut(s) 412, 4458
AccI GTMKAC 2 cut(s) 2725, 4723
AccII CGCG 2 cut(s) 3994, 4615
AccIII TCCGGA 2 cut(s) 1438, 1754
AclI AACGTT 2 cut(s) 1009, 2915
AclWI GGATC 6 cut(s) 341, 2377, 3200, 3213, 3316, 3504
AcoI YGGCCR 3 cut(s) 1198, 3120, 4659
AcsI RAATTY 7 cut(s) 47, 1148, 1748, 3028, 3057, 3581, 3689
AcuI CTGAAG 9 cut(s) 1061, 1089, 1578, 1995, 2183, 2819, 2859, 4264, 4455
AcvI CACGTG 1 cut(s) 953
AfiI CCNNNNNNNGG 3 cut(s) 41, 2042, 3876
AflII CTTAAG 5 cut(s) 1316, 1613, 2629, 2644, 3935
AflIII ACRYGT 2 cut(s) 1863, 2507
AgeI ACCGGT 1 cut(s) 3268
AhdI GACNNNNNGTC 1 cut(s) 320
AjiI CACGTC 1 cut(s) 3943
AjnI CCWGG 9 cut(s) 263, 973, 1564, 2881, 3551, 3709, 3928, 4107, 4661
AleI CACNNNNGTG 1 cut(s) 3614
AloI GAACNNNNNNTCC 2 cut(s) 2964, 2996
Alw21I GWGCWC 2 cut(s) 952, 4454
Alw26I GTCTC 9 cut(s) 479, 2043, 2273, 2354, 3524, 4339, 4559, 4751, 4805
Alw44I GTGCAC 1 cut(s) 948
AlwI GGATC 6 cut(s) 341, 2377, 3200, 3213, 3316, 3504
AlwNI CAGNNNCTG 1 cut(s) 2882
Ama87I CYCGRG 2 cut(s) 2387, 4562
Aor13HI TCCGGA 2 cut(s) 1438, 1754
AoxI GGCC 7 cut(s) 1198, 1423, 3005, 3120, 3289, 3947, 4659
ApaLI GTGCAC 1 cut(s) 948
ApeKI GCWGC 7 cut(s) 738, 2242, 2419, 2616, 2740, 3778, 4694
ApoI RAATTY 7 cut(s) 47, 1148, 1748, 3028, 3057, 3581, 3689
ArsI GACNNNNNNTTYG 2 cut(s) 498, 530
AseI ATTAAT 2 cut(s) 560, 1692
AsiGI ACCGGT 1 cut(s) 3268
Asp700I GAANNNNTTC 5 cut(s) 613, 1074, 2127, 2834, 4349
AspLEI GCGC 1 cut(s) 4617
AspS9I GGNCC 5 cut(s) 106, 188, 971, 2223, 2907
AsuII TTCGAA 2 cut(s) 566, 3026
AvaI CYCGRG 2 cut(s) 2387, 4562
AvaII GGWCC 5 cut(s) 106, 188, 971, 2223, 2907
BaeGI GKGCMC 3 cut(s) 952, 4231, 4717
BaeI ACNNNNGTAYC 2 cut(s) 3522, 3555
BalI TGGCCA 3 cut(s) 1200, 3122, 4661
BamHI GGATCC 1 cut(s) 3205
BanI GGYRCC 1 cut(s) 4226
BanII GRGCYC 1 cut(s) 724
BauI CACGAG 1 cut(s) 3614
BbrPI CACGTG 1 cut(s) 953
BbsI GAAGAC 4 cut(s) 18, 370, 2303, 2756
Bbv12I GWGCWC 2 cut(s) 952, 4454
BbvI GCAGC 7 cut(s) 750, 2254, 2431, 2628, 2727, 3765, 4706
BceAI ACGGC 4 cut(s) 1444, 1937, 3134, 4537
BciT130I CCWGG 9 cut(s) 265, 975, 1566, 2883, 3553, 3711, 3930, 4109, 4663
BcoDI GTCTC 9 cut(s) 479, 2043, 2273, 2354, 3524, 4339, 4559, 4751, 4805
BfaI CTAG 8 cut(s) 1418, 1488, 1988, 2564, 3293, 3345, 3449, 3893
BfmI CTRYAG 3 cut(s) 2480, 3048, 3791
BfrI CTTAAG 5 cut(s) 1316, 1613, 2629, 2644, 3935
BfuAI ACCTGC 1 cut(s) 1569
BglII AGATCT 1 cut(s) 1930
BisI GCNGC 9 cut(s) 739, 1876, 2243, 2420, 2617, 2741, 3008, 3779, 4695
BlsI GCNGC 9 cut(s) 740, 1877, 2244, 2421, 2618, 2742, 3009, 3780, 4696
Bme1390I CCNGG 9 cut(s) 265, 975, 1566, 2883, 3553, 3711, 3930, 4109, 4663
Bme18I GGWCC 5 cut(s) 106, 188, 971, 2223, 2907
BmeRI GACNNNNNGTC 1 cut(s) 320
BmeT110I CYCGRG 2 cut(s) 2387, 4562
BmgBI CACGTC 1 cut(s) 3943
BmgT120I GGNCC 5 cut(s) 106, 188, 971, 2223, 2907
BmiI GGNNCC 4 cut(s) 426, 2224, 3207, 4228
BmrFI CCNGG 9 cut(s) 265, 975, 1566, 2883, 3553, 3711, 3930, 4109, 4663
BmrI ACTGGG 2 cut(s) 718, 2310
BmuI ACTGGG 2 cut(s) 718, 2310
BoxI GACNNNNGTC 1 cut(s) 2855
BpiI GAAGAC 4 cut(s) 18, 370, 2303, 2756
BplI GAGNNNNNCTC 6 cut(s) 2131, 2163, 2586, 2618, 2995, 3027
BpmI CTGGAG 3 cut(s) 1947, 2225, 2435
Bpu14I TTCGAA 2 cut(s) 566, 3026
BpuEI CTTGAG 8 cut(s) 767, 1715, 2036, 2590, 2720, 2734, 3653, 3677
Bsa29I ATCGAT 1 cut(s) 182
BsaAI YACGTR 2 cut(s) 54, 953
BsaI GGTCTC 3 cut(s) 2273, 3524, 4805
BsaWI WCCGGW 6 cut(s) 41, 1438, 1754, 3202, 3268, 3541
Bsc4I CCNNNNNNNGG 3 cut(s) 41, 2042, 3876
Bse118I RCCGGY 1 cut(s) 3268
Bse3DI GCAATG 3 cut(s) 2460, 4050, 4655
BseAI TCCGGA 2 cut(s) 1438, 1754
BseBI CCWGG 9 cut(s) 265, 975, 1566, 2883, 3553, 3711, 3930, 4109, 4663
BseCI ATCGAT 1 cut(s) 182
BseLI CCNNNNNNNGG 3 cut(s) 41, 2042, 3876
BseMI GCAATG 3 cut(s) 2460, 4050, 4655
BseMII CTCAG 8 cut(s) 82, 345, 1169, 1925, 2940, 3375, 3712, 4037
BseRI GAGGAG 1 cut(s) 1190
BseSI GKGCMC 3 cut(s) 952, 4231, 4717
BseXI GCAGC 7 cut(s) 750, 2254, 2431, 2628, 2727, 3765, 4706
BsgI GTGCAG 2 cut(s) 804, 4713
Bsh1236I CGCG 2 cut(s) 3994, 4615
BshFI GGCC 7 cut(s) 1200, 1425, 3007, 3122, 3291, 3949, 4661
BshNI GGYRCC 1 cut(s) 4226
BshTI ACCGGT 1 cut(s) 3268
BshVI ATCGAT 1 cut(s) 182
BsiHKAI GWGCWC 2 cut(s) 952, 4454
BsiHKCI CYCGRG 2 cut(s) 2387, 4562
BsiSI CCGG 6 cut(s) 42, 1439, 1755, 3203, 3269, 3542
BsiWI CGTACG 1 cut(s) 54
BslFI GGGAC 4 cut(s) 327, 1116, 2236, 4304
BslI CCNNNNNNNGG 3 cut(s) 41, 2042, 3876
BsmAI GTCTC 9 cut(s) 479, 2043, 2273, 2354, 3524, 4339, 4559, 4751, 4805
BsmBI CGTCTC 1 cut(s) 2043
BsmFI GGGAC 4 cut(s) 327, 1116, 2236, 4304
BsmI GAATGC 1 cut(s) 615
BsnI GGCC 7 cut(s) 1200, 1425, 3007, 3122, 3291, 3949, 4661
Bso31I GGTCTC 3 cut(s) 2273, 3524, 4805
BsoBI CYCGRG 2 cut(s) 2387, 4562
Bsp119I TTCGAA 2 cut(s) 566, 3026
Bsp1286I GDGCHC 5 cut(s) 724, 952, 4231, 4454, 4717
Bsp13I TCCGGA 2 cut(s) 1438, 1754
Bsp1407I TGTACA 2 cut(s) 838, 2504
Bsp19I CCATGG 1 cut(s) 3420
BspANI GGCC 7 cut(s) 1200, 1425, 3007, 3122, 3291, 3949, 4661
BspCNI CTCAG 8 cut(s) 81, 346, 1168, 1926, 2939, 3376, 3711, 4036
BspDI ATCGAT 1 cut(s) 182
BspEI TCCGGA 2 cut(s) 1438, 1754
BspFNI CGCG 2 cut(s) 3994, 4615
BspHI TCATGA 1 cut(s) 3676
BspLI GGNNCC 4 cut(s) 426, 2224, 3207, 4228
BspMI ACCTGC 1 cut(s) 1569
BspPI GGATC 6 cut(s) 341, 2377, 3200, 3213, 3316, 3504
BspQI GCTCTTC 3 cut(s) 1554, 3791, 4465
BspT104I TTCGAA 2 cut(s) 566, 3026
BspT107I GGYRCC 1 cut(s) 4226
BspTI CTTAAG 5 cut(s) 1316, 1613, 2629, 2644, 3935
BspTNI GGTCTC 3 cut(s) 2273, 3524, 4805
BsrBI CCGCTC 2 cut(s) 412, 4458
BsrDI GCAATG 3 cut(s) 2460, 4050, 4655
BsrFI RCCGGY 1 cut(s) 3268
BsrGI TGTACA 2 cut(s) 838, 2504
BssAI RCCGGY 1 cut(s) 3268
BssSI CACGAG 1 cut(s) 3614
BssT1I CCWWGG 6 cut(s) 1593, 2118, 2497, 3304, 3420, 4369
Bst2BI CACGAG 1 cut(s) 3614
Bst2UI CCWGG 9 cut(s) 265, 975, 1566, 2883, 3553, 3711, 3930, 4109, 4663
Bst6I CTCTTC 4 cut(s) 434, 1554, 3791, 4465
BstAFI CTTAAG 5 cut(s) 1316, 1613, 2629, 2644, 3935
BstAPI GCANNNNNTGC 1 cut(s) 1634
BstAUI TGTACA 2 cut(s) 838, 2504
BstBAI YACGTR 2 cut(s) 54, 953
BstBI TTCGAA 2 cut(s) 566, 3026
BstC8I GCNNGC 6 cut(s) 1916, 2201, 2467, 3996, 4488, 4545
BstDSI CCRYGG 3 cut(s) 1426, 3420, 3992
BstFNI CGCG 2 cut(s) 3994, 4615
BstHHI GCGC 1 cut(s) 4617
BstMAI GTCTC 9 cut(s) 479, 2043, 2273, 2354, 3524, 4339, 4559, 4751, 4805
BstNI CCWGG 9 cut(s) 265, 975, 1566, 2883, 3553, 3711, 3930, 4109, 4663
BstNSI RCATGY 4 cut(s) 1377, 1867, 1918, 2511
BstPAI GACNNNNGTC 1 cut(s) 2855
BstSCI CCNGG 9 cut(s) 263, 973, 1564, 2881, 3551, 3709, 3928, 4107, 4661
BstSFI CTRYAG 3 cut(s) 2480, 3048, 3791
BstSLI GKGCMC 3 cut(s) 952, 4231, 4717
BstUI CGCG 2 cut(s) 3994, 4615
BstV1I GCAGC 7 cut(s) 750, 2254, 2431, 2628, 2727, 3765, 4706
BstV2I GAAGAC 4 cut(s) 18, 370, 2303, 2756
BstX2I RGATCY 3 cut(s) 1930, 2382, 3205
BstXI CCANNNNNNTGG 2 cut(s) 731, 3616
BstYI RGATCY 3 cut(s) 1930, 2382, 3205
Bsu15I ATCGAT 1 cut(s) 182
BsuRI GGCC 7 cut(s) 1200, 1425, 3007, 3122, 3291, 3949, 4661
BsuTUI ATCGAT 1 cut(s) 182
BtgI CCRYGG 3 cut(s) 1426, 3420, 3992
BtgZI GCGATG 1 cut(s) 1354
BtrI CACGTC 1 cut(s) 3943
BtsI GCAGTG 2 cut(s) 3769, 4803
BveI ACCTGC 1 cut(s) 1569
Cac8I GCNNGC 6 cut(s) 1916, 2201, 2467, 3996, 4488, 4545
CaiI CAGNNNCTG 1 cut(s) 2882
CciI TCATGA 1 cut(s) 3676
CfoI GCGC 1 cut(s) 4617
Cfr10I RCCGGY 1 cut(s) 3268
Cfr13I GGNCC 5 cut(s) 106, 188, 971, 2223, 2907
Cfr42I CCGCGG 1 cut(s) 3995
ClaI ATCGAT 1 cut(s) 182
CseI GACGC 2 cut(s) 1532, 2297
CspAI ACCGGT 1 cut(s) 3268
CspCI CAANNNNNGTGG 2 cut(s) 2004, 2039
DraI TTTAAA 2 cut(s) 709, 1384
DriI GACNNNNNGTC 1 cut(s) 320
EaeI YGGCCR 3 cut(s) 1198, 3120, 4659
Eam1104I CTCTTC 4 cut(s) 434, 1554, 3791, 4465
Eam1105I GACNNNNNGTC 1 cut(s) 320
EarI CTCTTC 4 cut(s) 434, 1554, 3791, 4465
EciI GGCGGA 1 cut(s) 3868
Eco130I CCWWGG 6 cut(s) 1593, 2118, 2497, 3304, 3420, 4369
Eco24I GRGCYC 1 cut(s) 724
Eco31I GGTCTC 3 cut(s) 2273, 3524, 4805
Eco32I GATATC 1 cut(s) 2559
Eco47I GGWCC 5 cut(s) 106, 188, 971, 2223, 2907
Eco57I CTGAAG 9 cut(s) 1061, 1089, 1578, 1995, 2183, 2819, 2859, 4264, 4455
Eco72I CACGTG 1 cut(s) 953
Eco88I CYCGRG 2 cut(s) 2387, 4562
EcoO109I RGGNCCY 1 cut(s) 2907
EcoRI GAATTC 1 cut(s) 3028
EcoRII CCWGG 9 cut(s) 263, 973, 1564, 2881, 3551, 3709, 3928, 4107, 4661
EcoRV GATATC 1 cut(s) 2559
EcoT14I CCWWGG 6 cut(s) 1593, 2118, 2497, 3304, 3420, 4369
EcoT22I ATGCAT 1 cut(s) 1509
EcoT38I GRGCYC 1 cut(s) 724
ErhI CCWWGG 6 cut(s) 1593, 2118, 2497, 3304, 3420, 4369
Esp3I CGTCTC 1 cut(s) 2043
FalI AAGNNNNNCTT 2 cut(s) 309, 341
FaqI GGGAC 4 cut(s) 327, 1116, 2236, 4304
FauI CCCGC 4 cut(s) 98, 3431, 3987, 4463
FblI GTMKAC 2 cut(s) 2725, 4723
Fnu4HI GCNGC 9 cut(s) 739, 1876, 2243, 2420, 2617, 2741, 3008, 3779, 4695
FriOI GRGCYC 1 cut(s) 724
Fsp4HI GCNGC 9 cut(s) 739, 1876, 2243, 2420, 2617, 2741, 3008, 3779, 4695
FspBI CTAG 8 cut(s) 1418, 1488, 1988, 2564, 3293, 3345, 3449, 3893
GlaI GCGC 1 cut(s) 4616
GluI GCNGC 9 cut(s) 739, 1876, 2243, 2420, 2617, 2741, 3008, 3779, 4695
GsuI CTGGAG 3 cut(s) 1947, 2225, 2435
HaeIII GGCC 7 cut(s) 1200, 1425, 3007, 3122, 3291, 3949, 4661
HapII CCGG 6 cut(s) 42, 1439, 1755, 3203, 3269, 3542
HgaI GACGC 2 cut(s) 1532, 2297
HhaI GCGC 1 cut(s) 4617
Hin6I GCGC 1 cut(s) 4615
HinP1I GCGC 1 cut(s) 4615
HincII GTYRAC 2 cut(s) 292, 1959
HindII GTYRAC 2 cut(s) 292, 1959
HindIII AAGCTT 3 cut(s) 2017, 2197, 2696
HpaII CCGG 6 cut(s) 42, 1439, 1755, 3203, 3269, 3542
Hpy99I CGWCG 1 cut(s) 3364
HpyCH4IV ACGT 9 cut(s) 53, 952, 1009, 1268, 1573, 2915, 3252, 3353, 3942
HpySE526I ACGT 9 cut(s) 53, 952, 1009, 1268, 1573, 2915, 3252, 3353, 3942
HspAI GCGC 1 cut(s) 4615
Kpn2I TCCGGA 2 cut(s) 1438, 1754
KspI CCGCGG 1 cut(s) 3995
LguI GCTCTTC 3 cut(s) 1554, 3791, 4465
LmnI GCTCC 8 cut(s) 376, 1911, 2206, 2372, 2416, 2594, 2884, 4457
Lsp1109I GCAGC 7 cut(s) 750, 2254, 2431, 2628, 2727, 3765, 4706
MaeI CTAG 8 cut(s) 1418, 1488, 1988, 2564, 3293, 3345, 3449, 3893
MaeII ACGT 9 cut(s) 53, 952, 1009, 1268, 1573, 2915, 3252, 3353, 3942
MaeIII GTNAC 6 cut(s) 1269, 1477, 1782, 2318, 2848, 3235
MbiI CCGCTC 2 cut(s) 412, 4458
MfeI CAATTG 5 cut(s) 525, 932, 2250, 2996, 4035
MflI RGATCY 3 cut(s) 1930, 2382, 3205
MhlI GDGCHC 5 cut(s) 724, 952, 4231, 4454, 4717
MlsI TGGCCA 3 cut(s) 1200, 3122, 4661
MluNI TGGCCA 3 cut(s) 1200, 3122, 4661
MlyI GAGTC 4 cut(s) 3539, 3644, 4341, 4496
MmeI TCCRAC 3 cut(s) 1099, 4414, 4707
Mox20I TGGCCA 3 cut(s) 1200, 3122, 4661
Mph1103I ATGCAT 1 cut(s) 1509
MroI TCCGGA 2 cut(s) 1438, 1754
MroXI GAANNNNTTC 5 cut(s) 613, 1074, 2127, 2834, 4349
MscI TGGCCA 3 cut(s) 1200, 3122, 4661
MslI CAYNNNNRTG 4 cut(s) 603, 2217, 3614, 4710
Msp20I TGGCCA 3 cut(s) 1200, 3122, 4661
MspA1I CMGCKG 1 cut(s) 3994
MspCI CTTAAG 5 cut(s) 1316, 1613, 2629, 2644, 3935
MspI CCGG 6 cut(s) 42, 1439, 1755, 3203, 3269, 3542
MspR9I CCNGG 9 cut(s) 265, 975, 1566, 2883, 3553, 3711, 3930, 4109, 4663
MunI CAATTG 5 cut(s) 525, 932, 2250, 2996, 4035
Mva1269I GAATGC 1 cut(s) 615
MvaI CCWGG 9 cut(s) 265, 975, 1566, 2883, 3553, 3711, 3930, 4109, 4663
MvnI CGCG 2 cut(s) 3994, 4615
NcoI CCATGG 1 cut(s) 3420
NlaIV GGNNCC 4 cut(s) 426, 2224, 3207, 4228
NmuCI GTSAC 2 cut(s) 2848, 3235
NsiI ATGCAT 1 cut(s) 1509
NspI RCATGY 4 cut(s) 1377, 1867, 1918, 2511
NspV TTCGAA 2 cut(s) 566, 3026
OliI CACNNNNGTG 1 cut(s) 3614
PaeI GCATGC 1 cut(s) 1918
PagI TCATGA 1 cut(s) 3676
PciI ACATGT 2 cut(s) 1863, 2507
PciSI GCTCTTC 3 cut(s) 1554, 3791, 4465
PcsI WCGNNNNNNNCGW 1 cut(s) 3359
PctI GAATGC 1 cut(s) 615
PdmI GAANNNNTTC 5 cut(s) 613, 1074, 2127, 2834, 4349
Pfl23II CGTACG 1 cut(s) 54
PfoI TCCNGGA 2 cut(s) 2881, 3928
PinAI ACCGGT 1 cut(s) 3268
PkrI GCNGC 9 cut(s) 740, 1877, 2244, 2421, 2618, 2742, 3009, 3780, 4696
PleI GAGTC 4 cut(s) 3539, 3643, 4340, 4496
PmaCI CACGTG 1 cut(s) 953
PmlI CACGTG 1 cut(s) 953
PpsI GAGTC 4 cut(s) 3539, 3643, 4340, 4496
Ppu21I YACGTR 2 cut(s) 54, 953
PpuMI RGGWCCY 1 cut(s) 2907
PscI ACATGT 2 cut(s) 1863, 2507
PshAI GACNNNNGTC 1 cut(s) 2855
PshBI ATTAAT 2 cut(s) 560, 1692
PsiI TTATAA 1 cut(s) 1409
Psp1406I AACGTT 2 cut(s) 1009, 2915
Psp5II RGGWCCY 1 cut(s) 2907
Psp6I CCWGG 9 cut(s) 263, 973, 1564, 2881, 3551, 3709, 3928, 4107, 4661
PspCI CACGTG 1 cut(s) 953
PspGI CCWGG 9 cut(s) 263, 973, 1564, 2881, 3551, 3709, 3928, 4107, 4661
PspLI CGTACG 1 cut(s) 54
PspN4I GGNNCC 4 cut(s) 426, 2224, 3207, 4228
PspPI GGNCC 5 cut(s) 106, 188, 971, 2223, 2907
PspPPI RGGWCCY 1 cut(s) 2907
PsrI GAACNNNNNNTAC 2 cut(s) 47, 79
PstNI CAGNNNCTG 1 cut(s) 2882
PsuI RGATCY 3 cut(s) 1930, 2382, 3205
RseI CAYNNNNRTG 4 cut(s) 603, 2217, 3614, 4710
SacII CCGCGG 1 cut(s) 3995
SapI GCTCTTC 3 cut(s) 1554, 3791, 4465
SatI GCNGC 9 cut(s) 739, 1876, 2243, 2420, 2617, 2741, 3008, 3779, 4695
Sau96I GGNCC 5 cut(s) 106, 188, 971, 2223, 2907
SchI GAGTC 4 cut(s) 3539, 3644, 4341, 4496
ScrFI CCNGG 9 cut(s) 265, 975, 1566, 2883, 3553, 3711, 3930, 4109, 4663
SduI GDGCHC 5 cut(s) 724, 952, 4231, 4454, 4717
SfcI CTRYAG 3 cut(s) 2480, 3048, 3791
Sfr303I CCGCGG 1 cut(s) 3995
SfuI TTCGAA 2 cut(s) 566, 3026
SgrBI CCGCGG 1 cut(s) 3995
SinI GGWCC 5 cut(s) 106, 188, 971, 2223, 2907
SmiMI CAYNNNNRTG 4 cut(s) 603, 2217, 3614, 4710
SphI GCATGC 1 cut(s) 1918
SspI AATATT 2 cut(s) 942, 3486
SspMI CTAG 8 cut(s) 1418, 1488, 1988, 2564, 3293, 3345, 3449, 3893
StyD4I CCNGG 9 cut(s) 263, 973, 1564, 2881, 3551, 3709, 3928, 4107, 4661
StyI CCWWGG 6 cut(s) 1593, 2118, 2497, 3304, 3420, 4369
TaiI ACGT 9 cut(s) 56, 955, 1012, 1271, 1576, 2918, 3255, 3356, 3945
TaqII GACCGA 1 cut(s) 4787
TatI WGTACW 5 cut(s) 838, 1609, 2504, 2789, 3040
TauI GCSGC 2 cut(s) 1878, 3010
TseFI GTSAC 2 cut(s) 2848, 3235
TseI GCWGC 7 cut(s) 738, 2242, 2419, 2616, 2740, 3778, 4694
Tsp45I GTSAC 2 cut(s) 2848, 3235
TspGWI ACGGA 7 cut(s) 119, 1595, 1646, 2745, 3181, 3348, 4085
Vha464I CTTAAG 5 cut(s) 1316, 1613, 2629, 2644, 3935
VneI GTGCAC 1 cut(s) 948
VpaK11BI GGWCC 5 cut(s) 106, 188, 971, 2223, 2907
VspI ATTAAT 2 cut(s) 560, 1692
XapI RAATTY 7 cut(s) 47, 1148, 1748, 3028, 3057, 3581, 3689
XbaI TCTAGA 4 cut(s) 1487, 1987, 3344, 3892
XceI RCATGY 4 cut(s) 1377, 1867, 1918, 2511
XcmI CCANNNNNNNNNTGG 1 cut(s) 3418
XmiI GTMKAC 2 cut(s) 2725, 4723
XmnI GAANNNNTTC 5 cut(s) 613, 1074, 2127, 2834, 4349
XspI CTAG 8 cut(s) 1418, 1488, 1988, 2564, 3293, 3345, 3449, 3893
Zsp2I ATGCAT 1 cut(s) 1509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.