RLG00000001970

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000129
Physical Location & Seq
Reverse (-)
23744468 .. 23750431
5964 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001970

Sequence Viewer

Length: 3249 bp
ATGGCCTATCCAGTCCGCCTTCTCCTCTTGTCTTCCATTTGCCCTCTAGTTTTCTTCTCTGCTTTGTTTCTCTTGAGCTTTATCTCTTTACGGTTGTACCTGTTGTTCTTCTTCTCCGCTGTTTGTATTTGCTGCTGCCTTTTCGCTTTCAAACATAGACCAACAACACTACTCCACACTAAACCTTCAGTGACCACCAAAACAGATATTCCATCTTCTTCTTCTTCTCCTCCTCCTCCTCCATCCCTTAAATACGATGTGTTCCTCAGTTTTAGAGGTACAGATACCCGCAAAAATTTCACGGACCATCTTTATACTGCTCTCAAACAGAATGGTATATTCACTTTTAGAGATGATGAAGAGCTCGAGAGAGGGGAATCGATTGGTCCAAATCTGTTGAAAGCAATAGAGGAATCAAGATATGTAATTGCTGTTTTCTCACGAAACTATGCCGATTCTACATGGTGTTTGGATGAGATTGCCAAGGTCGCGGATTGCATGAAAGACATGGGACAAAAAGTCCTCCCAGTGTTCTACGATGTTGATCCAAATGAGGTACGAAGACAAACAGGGGATCACTTTGGGAAAGCATTTGAGAAGCATCAAAAGCGGTATAAAGCGAAACCAGAAAAAGTAAAGAGGTGGAAAGACGCTCTTTTTCAAGTAGGCAATCTCTCTGGATGGCATTTGCAAGATGGGTACGAATCCAAAGTCATTCAAGAAATTGTTGAAAAGATTTTCACCGAGTTGAATCATATAATCCCAATATCTGAGGGTTTAGTTGGGATGGATTCTCACCTGAATGAAATGCTTTCGTACTTAGACATTGGTTGTCCCGATGTTCGTATCATAGGGATTTGTGGGATGGGTGGTATCGGTAAGACAACTATTGCACAAGTGGTTTTTGAAACGGTAAAAGCTCATTTTGAAGGTTGCAGCTTTCTTGAGAATGTGAGAGAGGTAACTGAAAAGCAAGGTGCAATTCATTTACAACAGAACCTTCTTTCAGATTTGCTGAAATGTAGTGTAAATGTACAGAACACTAAAATGGGAACAAGTATAATGAGGCAGAGACTATGTACTAAAATGGTTCTTATCATTCTTGATGATGTGGACCAAGAAGAACAATTGGAAGCACTGTGTGATCGTGAATCATTTGGTCCAGGGAGTAGAATCATTATAACGTCAAGAGATGAACATTTGCTGAGTGCAGTTGAAGGAGATAAAGTGTACAATGTGAATTTGTTAACTCCTGCCGAAGCTCGTGAGCTCTTTGGTATGAAAGCCTTTAAGAAATACCAGCCAGTTGGGAAAGATTTTCTCGAGCTATCTGAGGAATTTTTGAAATATGCTAATGGCCTTCCATTAGCTATTAAAGTTTTGGGTTCCTCTGTTCGTGGTAGAAGTGTAAAATTATGGTCTAGTACATTGGATAGAATAAAAAACAATCCTCAAAAAGGGGTTATTGATGTGCTTAAATCTAGTTTTGAGGGACTAGAAGAAACCGAAAAGAAAATGTTTTTGGACATCGCATGTTTCTTTAAAGGAGGGTGCATAGATCAGGTAACAAGAATACTACAAGGTAGTGACCACTGTCCGGACATTGATATAGAAGTTCTGGTAAATAAATCTTTGGTAACTCTGTTTGGAAGAAAATTGTTGATGCATGACTTGGTACAAGAATTGGGTTGGGACATTGTTCGTCAAGAATGCCTTGAAGAGCCTGGAAAACGTAGTACGTTGTGGCTTCCCAATGAAATCATTCGTGTACTTGATCGGTGTAAGGCGACAGATGCTGTTGAAAGCATATACCTCCAATGTCCAACAGAAGACGATGTTGTCCACTCAATTGATAATGCCCTCTCACAGATGGACAGATTGAGATTGCTTAAGATTTGTAATGTGAAGTTTTTTAGAAACATCACATATCTTTCTAATGAGTTACAATATCTTGAATGGCATGAATGTCCTTTGGATTCTTTCCCGTCAGACTTTCAACCGAATAAACTTGTTGAACTTCATATGTATTTTAGCCGCATCAAACAACTATGGAAGGGAAAAAAAGGATGGAGCATGCTACGGCATATTGATCTGTCGGGTTCACAATACTTGATGTCAACTCCAGACTTCACTGAGGTTCCTGATCTTGAAGATTTGGTGCTTCAAAATTGCACTAGTTTGGTGGAGGTTCACCCATCTCTTGGATTTCTGAAGAAGCTTTGCTATTTGGATATGACAAATTGCAAATCTATTGAGAGCCTTCCACCTTTCACTAGCTTGGAATCCCTTGAAAAATTATGGCTTTCACTCTGTTCAGAACTGAAGAAATTTCCAGAAATTGAAGGAAATATGAAAAGCTTGCTGGAGCTTCATTTGGATGGGACTAGCATTGAGGAATTGCCCCCATCAATTGAACGGTTGACTGGTCTTACCATGTTGAATCTAGCAGACTGTCAAAACCTTTTGCTTCTTCCCAATACCATTGGGTGCTTGACATCTTTGAAAAGTCTCGATCTAACAGGTTGCTCCAAAATCGAAGAGATACCTGAGAATCTGAATGGTATGAAATGTCTGGAGAAGCTTTTAATTGGTGAAACTTCCATAAGGGACTTATCTTTCATTGTAGGCATGAAGGATCTACAATATCTATCCTGCCGAGGATGTAAATGTGTCGTTTCAGAATCATGCAAAGGTCTGGCTTCGTTATCGAATTTGATAGAGTTAGACTTGAGTTATTGCAATCTCATGTATGGAGAAATTCTCAATGATTTTAGCAGACTAATCTCCTTAAGAGAGTTAAATTTAAGTGGTAATTGCTTTGTGCGATTACCTGAAAGCATCTCTCAACTCTCAAAGCTCGAGATTCTGAACTTGAGGAATTGCAGGCAGCTTCAATTGCTGCCCAAGAAGCTTCCGTTAAGTCTTCAAAACGTGCATGCAGAAGGATGTACTTCACTGATGGATTACCCAAATCAAATCAAAGTATTGAATTCATGGGAGTCGGGAGTGACTATTGTCAATTCCCTCAATTCTTCAAAACTCGGAGCCTCACAAAGTTACCGAATGTCGGTGATTCCAAAAAAGGAGGGAGGATCTCCAGAGCTGCGTGTGACGTATCCTGAGGGGAAACAAGTTGATCACTTGCTACTGACAAATCTAACACTTCTAAAACAAGAAGTCTCTCTCTCTCTCTCTCATAACAAAAACAGAGCATATATCTATAATTCCATCTTTTTTTCATGCTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1083

Amino Acids

122.53

Weight (kDa)

5.97

Isoelectric Point (pI)

45.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 84 - 256 2e-55 TIR domain
TIR_2 PF13676 87 - 186 1.6e-16 TIR domain
NB-ARC PF00931 267 - 432 6.5e-23 NB-ARC domain
WHD_ROQ1 PF23282 500 - 569 8.1e-17 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 728 - 840 7.2e-09 Leucine-rich repeat region
LRR_13 PF23286 760 - 847 5.7e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 904 - 998 1.7e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1181
AasI GACNNNNNNGTC 1 cut(s) 1838
AccB7I CCANNNNNTGG 1 cut(s) 2201
AccII CGCG 1 cut(s) 491
AccIII TCCGGA 1 cut(s) 1597
AciI CCGC 6 cut(s) 16, 117, 289, 491, 610, 2035
AclWI GGATC 4 cut(s) 539, 582, 2643, 3100
AcsI RAATTY 8 cut(s) 295, 1240, 1337, 2327, 2710, 2757, 2800, 2989
AcuI CTGAAG 3 cut(s) 171, 2231, 2342
AdeI CACNNNGTG 1 cut(s) 1142
AfiI CCNNNNNNNGG 4 cut(s) 1457, 1597, 2201, 3067
AflII CTTAAG 2 cut(s) 1889, 2788
AhdI GACNNNNNGTC 1 cut(s) 518
AhlI ACTAGT 1 cut(s) 2174
AjnI CCWGG 2 cut(s) 1162, 1723
AjuI GAANNNNNNNTTGG 2 cut(s) 1505, 1537
Alw21I GWGCWC 2 cut(s) 366, 1272
Alw26I GTCTC 3 cut(s) 1066, 2513, 3184
AlwI GGATC 4 cut(s) 539, 582, 2643, 3100
AlwNI CAGNNNCTG 1 cut(s) 1796
Ama87I CYCGRG 3 cut(s) 365, 1322, 2858
Aor13HI TCCGGA 1 cut(s) 1597
AoxI GGCC 2 cut(s) 3, 1357
ApeKI GCWGC 6 cut(s) 132, 135, 936, 2887, 2899, 3103
ApoI RAATTY 8 cut(s) 295, 1240, 1337, 2327, 2710, 2757, 2800, 2989
ArsI GACNNNNNNTTYG 2 cut(s) 696, 728
Asp700I GAANNNNTTC 1 cut(s) 1761
AspS9I GGNCC 4 cut(s) 304, 386, 1114, 1160
AsuHPI GGTGA 5 cut(s) 733, 788, 2183, 2603, 3082
AvaI CYCGRG 3 cut(s) 365, 1322, 2858
AvaII GGWCC 4 cut(s) 304, 386, 1114, 1160
AxyI CCTNAGG 1 cut(s) 3120
BaeI ACNNNNGTAYC 2 cut(s) 80, 113
BanII GRGCYC 2 cut(s) 366, 1272
BauI CACGAG 1 cut(s) 1263
BbsI GAAGAC 4 cut(s) 24, 568, 1836, 2915
Bbv12I GWGCWC 2 cut(s) 366, 1272
BbvI GCAGC 6 cut(s) 119, 122, 948, 2886, 2899, 3090
BceAI ACGGC 1 cut(s) 2096
BciT130I CCWGG 2 cut(s) 1164, 1725
BciVI GTATCC 1 cut(s) 3126
BclI TGATCA 1 cut(s) 3136
BcoDI GTCTC 3 cut(s) 1066, 2513, 3184
BcuI ACTAGT 1 cut(s) 2174
BfaI CTAG 9 cut(s) 47, 1422, 1482, 1496, 2175, 2274, 2385, 2444, 3247
BfrI CTTAAG 2 cut(s) 1889, 2788
BfuI GTATCC 1 cut(s) 3126
BisI GCNGC 7 cut(s) 133, 136, 937, 2035, 2888, 2900, 3104
BlsI GCNGC 7 cut(s) 134, 137, 938, 2036, 2889, 2901, 3105
Bme1390I CCNGG 2 cut(s) 1164, 1725
Bme18I GGWCC 4 cut(s) 304, 386, 1114, 1160
BmeRI GACNNNNNGTC 1 cut(s) 518
BmeT110I CYCGRG 3 cut(s) 365, 1322, 2858
BmgT120I GGNCC 4 cut(s) 304, 386, 1114, 1160
BmiI GGNNCC 3 cut(s) 1387, 2139, 3046
BmrFI CCNGG 2 cut(s) 1164, 1725
BmrI ACTGGG 1 cut(s) 521
BmsI GCATC 5 cut(s) 610, 1653, 1783, 2046, 2847
BmuI ACTGGG 1 cut(s) 521
BoxI GACNNNNGTC 2 cut(s) 1593, 3014
BpiI GAAGAC 4 cut(s) 24, 568, 1836, 2915
BplI GAGNNNNNCTC 2 cut(s) 2745, 2777
BpmI CTGGAG 4 cut(s) 2106, 2384, 2594, 3081
BpuEI CTTGAG 4 cut(s) 94, 965, 2749, 2893
Bsa29I ATCGAT 1 cut(s) 380
BsaBI GATNNNNATC 1 cut(s) 543
BsaJI CCNNGG 3 cut(s) 483, 1163, 2656
BsaWI WCCGGW 1 cut(s) 1597
BsaXI ACNNNNNCTCC 2 cut(s) 1212, 1242
Bsc4I CCNNNNNNNGG 4 cut(s) 1457, 1597, 2201, 3067
Bse1I ACTGG 4 cut(s) 11, 527, 1304, 2428
Bse21I CCTNAGG 1 cut(s) 3120
Bse8I GATNNNNATC 1 cut(s) 543
BseAI TCCGGA 1 cut(s) 1597
BseBI CCWGG 2 cut(s) 1164, 1725
BseCI ATCGAT 1 cut(s) 380
BseDI CCNNGG 3 cut(s) 483, 1163, 2656
BseGI GGATG 9 cut(s) 242, 478, 686, 792, 870, 2072, 2383, 2666, 2951
BseJI GATNNNNATC 1 cut(s) 543
BseLI CCNNNNNNNGG 4 cut(s) 1457, 1597, 2201, 3067
BseMII CTCAG 7 cut(s) 280, 762, 1196, 1323, 2124, 2538, 3111
BseNI ACTGG 4 cut(s) 11, 527, 1304, 2428
BseRI GAGGAG 5 cut(s) 14, 219, 222, 225, 228
BseXI GCAGC 6 cut(s) 119, 122, 948, 2886, 2899, 3090
BsgI GTGCAG 1 cut(s) 1230
Bsh1236I CGCG 1 cut(s) 491
BshFI GGCC 2 cut(s) 5, 1359
BshVI ATCGAT 1 cut(s) 380
BsiHKAI GWGCWC 2 cut(s) 366, 1272
BsiHKCI CYCGRG 3 cut(s) 365, 1322, 2858
BsiSI CCGG 1 cut(s) 1598
BslFI GGGAC 6 cut(s) 525, 819, 1506, 1706, 2395, 2621
BslI CCNNNNNNNGG 4 cut(s) 1457, 1597, 2201, 3067
BsmAI GTCTC 3 cut(s) 1066, 2513, 3184
BsmFI GGGAC 6 cut(s) 525, 819, 1506, 1706, 2395, 2621
BsmI GAATGC 1 cut(s) 1715
BsnI GGCC 2 cut(s) 5, 1359
BsoBI CYCGRG 3 cut(s) 365, 1322, 2858
Bsp1286I GDGCHC 2 cut(s) 366, 1272
Bsp13I TCCGGA 1 cut(s) 1597
Bsp1407I TGTACA 2 cut(s) 1033, 1230
BspACI CCGC 6 cut(s) 16, 117, 289, 491, 610, 2035
BspANI GGCC 2 cut(s) 5, 1359
BspCNI CTCAG 7 cut(s) 279, 763, 1197, 1324, 2125, 2539, 3112
BspDI ATCGAT 1 cut(s) 380
BspEI TCCGGA 1 cut(s) 1597
BspFNI CGCG 1 cut(s) 491
BspLI GGNNCC 3 cut(s) 1387, 2139, 3046
BspPI GGATC 4 cut(s) 539, 582, 2643, 3100
BspQI GCTCTTC 2 cut(s) 354, 1713
BspTI CTTAAG 2 cut(s) 1889, 2788
BsrGI TGTACA 2 cut(s) 1033, 1230
BsrI ACTGG 4 cut(s) 11, 527, 1304, 2428
BssECI CCNNGG 3 cut(s) 483, 1163, 2656
BssSI CACGAG 1 cut(s) 1263
BssT1I CCWWGG 1 cut(s) 483
Bst2BI CACGAG 1 cut(s) 1263
Bst2UI CCWGG 2 cut(s) 1164, 1725
Bst4CI ACNGT 6 cut(s) 93, 913, 1140, 1595, 2418, 2453
Bst6I CTCTTC 3 cut(s) 354, 1713, 2532
BstAFI CTTAAG 2 cut(s) 1889, 2788
BstAUI TGTACA 2 cut(s) 1033, 1230
BstC8I GCNNGC 4 cut(s) 2075, 2360, 2885, 2937
BstDEI CTNAG 8 cut(s) 266, 771, 820, 1205, 1332, 2133, 2547, 3120
BstENI CCTNNNNNAGG 1 cut(s) 1455
BstF5I GGATG 9 cut(s) 242, 478, 686, 792, 870, 2072, 2383, 2666, 2951
BstFNI CGCG 1 cut(s) 491
BstMAI GTCTC 3 cut(s) 1066, 2513, 3184
BstMWI GCNNNNNNNGC 5 cut(s) 488, 607, 1793, 2896, 2908
BstNI CCWGG 2 cut(s) 1164, 1725
BstNSI RCATGY 3 cut(s) 1536, 2077, 2939
BstPAI GACNNNNGTC 2 cut(s) 1593, 3014
BstSCI CCNGG 2 cut(s) 1162, 1723
BstUI CGCG 1 cut(s) 491
BstV1I GCAGC 6 cut(s) 119, 122, 948, 2886, 2899, 3090
BstV2I GAAGAC 4 cut(s) 24, 568, 1836, 2915
BstX2I RGATCY 2 cut(s) 2635, 3092
BstXI CCANNNNNNTGG 1 cut(s) 1307
BstYI RGATCY 2 cut(s) 2635, 3092
Bsu15I ATCGAT 1 cut(s) 380
Bsu36I CCTNAGG 1 cut(s) 3120
BsuI GTATCC 1 cut(s) 3126
BsuRI GGCC 2 cut(s) 5, 1359
BsuTUI ATCGAT 1 cut(s) 380
BtgZI GCGATG 1 cut(s) 1513
BtsCI GGATG 9 cut(s) 242, 478, 686, 792, 870, 2072, 2383, 2666, 2951
BtsIMutI CAGTG 6 cut(s) 195, 534, 1136, 1591, 2130, 2954
Cac8I GCNNGC 4 cut(s) 2075, 2360, 2885, 2937
CaiI CAGNNNCTG 1 cut(s) 1796
Cfr13I GGNCC 4 cut(s) 304, 386, 1114, 1160
ClaI ATCGAT 1 cut(s) 380
CseI GACGC 1 cut(s) 659
DdeI CTNAG 8 cut(s) 266, 771, 820, 1205, 1332, 2133, 2547, 3120
DraI TTTAAA 1 cut(s) 1543
DraIII CACNNNGTG 1 cut(s) 1142
DrdI GACNNNNNNGTC 1 cut(s) 1838
DriI GACNNNNNGTC 1 cut(s) 518
DseDI GACNNNNNNGTC 1 cut(s) 1838
Eam1104I CTCTTC 3 cut(s) 354, 1713, 2532
Eam1105I GACNNNNNGTC 1 cut(s) 518
EarI CTCTTC 3 cut(s) 354, 1713, 2532
EciI GGCGGA 1 cut(s) 5
Ecl136II GAGCTC 2 cut(s) 364, 1270
Eco130I CCWWGG 1 cut(s) 483
Eco24I GRGCYC 2 cut(s) 366, 1272
Eco47I GGWCC 4 cut(s) 304, 386, 1114, 1160
Eco53kI GAGCTC 2 cut(s) 364, 1270
Eco57I CTGAAG 3 cut(s) 171, 2231, 2342
Eco81I CCTNAGG 1 cut(s) 3120
Eco88I CYCGRG 3 cut(s) 365, 1322, 2858
EcoICRI GAGCTC 2 cut(s) 364, 1270
EcoNI CCTNNNNNAGG 1 cut(s) 1455
EcoRI GAATTC 1 cut(s) 2989
EcoRII CCWGG 2 cut(s) 1162, 1723
EcoT14I CCWWGG 1 cut(s) 483
EcoT22I ATGCAT 1 cut(s) 1668
EcoT38I GRGCYC 2 cut(s) 366, 1272
ErhI CCWWGG 1 cut(s) 483
FalI AAGNNNNNCTT 4 cut(s) 639, 671, 1698, 1730
FaqI GGGAC 6 cut(s) 525, 819, 1506, 1706, 2395, 2621
FauI CCCGC 1 cut(s) 296
FauNDI CATATG 1 cut(s) 2022
FbaI TGATCA 1 cut(s) 3136
Fnu4HI GCNGC 7 cut(s) 133, 136, 937, 2035, 2888, 2900, 3104
FokI GGATG 9 cut(s) 229, 485, 693, 799, 877, 2079, 2390, 2673, 2958
FriOI GRGCYC 2 cut(s) 366, 1272
Fsp4HI GCNGC 7 cut(s) 133, 136, 937, 2035, 2888, 2900, 3104
FspBI CTAG 9 cut(s) 47, 1422, 1482, 1496, 2175, 2274, 2385, 2444, 3247
GluI GCNGC 7 cut(s) 133, 136, 937, 2035, 2888, 2900, 3104
GsuI CTGGAG 4 cut(s) 2106, 2384, 2594, 3081
HaeIII GGCC 2 cut(s) 5, 1359
HapII CCGG 1 cut(s) 1598
HgaI GACGC 1 cut(s) 659
HincII GTYRAC 3 cut(s) 1248, 2118, 2421
HindII GTYRAC 3 cut(s) 1248, 2118, 2421
HindIII AAGCTT 4 cut(s) 2216, 2356, 2579, 2909
HpaI GTTAAC 1 cut(s) 1248
HpaII CCGG 1 cut(s) 1598
HphI GGTGA 5 cut(s) 733, 788, 2183, 2603, 3082
Hpy166II GTNNAC 9 cut(s) 1114, 1231, 1248, 1769, 1843, 2102, 2118, 2191, 2421
Hpy8I GTNNAC 9 cut(s) 1114, 1231, 1248, 1769, 1843, 2102, 2118, 2191, 2421
HpyCH4III ACNGT 6 cut(s) 93, 913, 1140, 1595, 2418, 2453
HpyCH4IV ACGT 5 cut(s) 1184, 1732, 1739, 2931, 3113
HpyF10VI GCNNNNNNNGC 5 cut(s) 488, 607, 1793, 2896, 2908
HpyF3I CTNAG 8 cut(s) 266, 771, 820, 1205, 1332, 2133, 2547, 3120
HpySE526I ACGT 5 cut(s) 1184, 1732, 1739, 2931, 3113
Kpn2I TCCGGA 1 cut(s) 1597
Ksp22I TGATCA 1 cut(s) 3136
KspAI GTTAAC 1 cut(s) 1248
LguI GCTCTTC 2 cut(s) 354, 1713
LmnI GCTCC 4 cut(s) 2070, 2365, 2531, 3044
Lsp1109I GCAGC 6 cut(s) 119, 122, 948, 2886, 2899, 3090
LweI GCATC 5 cut(s) 610, 1653, 1783, 2046, 2847
MaeI CTAG 9 cut(s) 47, 1422, 1482, 1496, 2175, 2274, 2385, 2444, 3247
MaeII ACGT 5 cut(s) 1184, 1732, 1739, 2931, 3113
MaeIII GTNAC 9 cut(s) 190, 961, 1564, 1586, 1636, 1941, 3007, 3056, 3109
MfeI CAATTG 4 cut(s) 1127, 1848, 2409, 2894
MflI RGATCY 2 cut(s) 2635, 3092
MhlI GDGCHC 2 cut(s) 366, 1272
MlyI GAGTC 1 cut(s) 3008
MmeI TCCRAC 1 cut(s) 1847
Mph1103I ATGCAT 1 cut(s) 1668
MroI TCCGGA 1 cut(s) 1597
MroXI GAANNNNTTC 1 cut(s) 1761
MslI CAYNNNNRTG 3 cut(s) 801, 1046, 2376
MspA1I CMGCKG 1 cut(s) 119
MspCI CTTAAG 2 cut(s) 1889, 2788
MspI CCGG 1 cut(s) 1598
MspR9I CCNGG 2 cut(s) 1164, 1725
MunI CAATTG 4 cut(s) 1127, 1848, 2409, 2894
Mva1269I GAATGC 1 cut(s) 1715
MvaI CCWGG 2 cut(s) 1164, 1725
MvnI CGCG 1 cut(s) 491
MwoI GCNNNNNNNGC 5 cut(s) 488, 607, 1793, 2896, 2908
NdeI CATATG 1 cut(s) 2022
NlaIV GGNNCC 3 cut(s) 1387, 2139, 3046
NmeAIII GCCGAG 1 cut(s) 2681
NmuCI GTSAC 4 cut(s) 190, 1586, 3007, 3109
NsiI ATGCAT 1 cut(s) 1668
NspI RCATGY 3 cut(s) 1536, 2077, 2939
PaeI GCATGC 2 cut(s) 2077, 2939
PaeR7I CTCGAG 3 cut(s) 365, 1322, 2858
PciSI GCTCTTC 2 cut(s) 354, 1713
PctI GAATGC 1 cut(s) 1715
PdmI GAANNNNTTC 1 cut(s) 1761
PflMI CCANNNNNTGG 1 cut(s) 2201
PkrI GCNGC 7 cut(s) 134, 137, 938, 2036, 2889, 2901, 3105
PleI GAGTC 1 cut(s) 3007
PpsI GAGTC 1 cut(s) 3007
PshAI GACNNNNGTC 2 cut(s) 1593, 3014
PsiI TTATAA 1 cut(s) 1181
Psp124BI GAGCTC 2 cut(s) 366, 1272
Psp6I CCWGG 2 cut(s) 1162, 1723
PspGI CCWGG 2 cut(s) 1162, 1723
PspN4I GGNNCC 3 cut(s) 1387, 2139, 3046
PspPI GGNCC 4 cut(s) 304, 386, 1114, 1160
PsrI GAACNNNNNNTAC 2 cut(s) 89, 121
PstNI CAGNNNCTG 1 cut(s) 1796
PsuI RGATCY 2 cut(s) 2635, 3092
RseI CAYNNNNRTG 3 cut(s) 801, 1046, 2376
SacI GAGCTC 2 cut(s) 366, 1272
SapI GCTCTTC 2 cut(s) 354, 1713
SatI GCNGC 7 cut(s) 133, 136, 937, 2035, 2888, 2900, 3104
Sau96I GGNCC 4 cut(s) 304, 386, 1114, 1160
SchI GAGTC 1 cut(s) 3008
ScrFI CCNGG 2 cut(s) 1164, 1725
SduI GDGCHC 2 cut(s) 366, 1272
SfaNI GCATC 5 cut(s) 610, 1653, 1783, 2046, 2847
Sfr274I CTCGAG 3 cut(s) 365, 1322, 2858
SinI GGWCC 4 cut(s) 304, 386, 1114, 1160
SlaI CTCGAG 3 cut(s) 365, 1322, 2858
SmiMI CAYNNNNRTG 3 cut(s) 801, 1046, 2376
SmlI CTYRAG 9 cut(s) 73, 365, 944, 1322, 1889, 2728, 2788, 2858, 2872
SmoI CTYRAG 9 cut(s) 73, 365, 944, 1322, 1889, 2728, 2788, 2858, 2872
SpeI ACTAGT 1 cut(s) 2174
SphI GCATGC 2 cut(s) 2077, 2939
SsiI CCGC 6 cut(s) 16, 117, 289, 491, 610, 2035
SspMI CTAG 9 cut(s) 47, 1422, 1482, 1496, 2175, 2274, 2385, 2444, 3247
SstI GAGCTC 2 cut(s) 366, 1272
StyD4I CCNGG 2 cut(s) 1162, 1723
StyI CCWWGG 1 cut(s) 483
TaaI ACNGT 6 cut(s) 93, 913, 1140, 1595, 2418, 2453
TaiI ACGT 5 cut(s) 1187, 1735, 1742, 2934, 3116
TaqI TCGA 7 cut(s) 366, 380, 1323, 2511, 2535, 2708, 2859
TatI WGTACW 6 cut(s) 1033, 1079, 1230, 1424, 1768, 2948
TauI GCSGC 1 cut(s) 2037
TscAI CASTG 6 cut(s) 195, 534, 1143, 1598, 2137, 2961
TseFI GTSAC 4 cut(s) 190, 1586, 3007, 3109
TseI GCWGC 6 cut(s) 132, 135, 936, 2887, 2899, 3103
Tsp45I GTSAC 4 cut(s) 190, 1586, 3007, 3109
TspGWI ACGGA 2 cut(s) 317, 2904
TspRI CASTG 6 cut(s) 195, 534, 1143, 1598, 2137, 2961
Van91I CCANNNNNTGG 1 cut(s) 2201
Vha464I CTTAAG 2 cut(s) 1889, 2788
VpaK11BI GGWCC 4 cut(s) 304, 386, 1114, 1160
XagI CCTNNNNNAGG 1 cut(s) 1455
XapI RAATTY 8 cut(s) 295, 1240, 1337, 2327, 2710, 2757, 2800, 2989
XceI RCATGY 3 cut(s) 1536, 2077, 2939
XhoI CTCGAG 3 cut(s) 365, 1322, 2858
XmnI GAANNNNTTC 1 cut(s) 1761
XspI CTAG 9 cut(s) 47, 1422, 1482, 1496, 2175, 2274, 2385, 2444, 3247
Zsp2I ATGCAT 1 cut(s) 1668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.