Rroxscaffold_2G00121840

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000129
Physical Location & Seq
Forward (+)
54618783 .. 54621821
3039 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00121840.1

Sequence Viewer

Length: 1017 bp
ATGAAATGTCTGGAGATGCTTTCCATTGGTGGAACTTCCATAAGGGAGTTATCCTTTATTGAAGGCATGAAGAATCTACGGTATCTATCCTGCCGAGGATGTGAACGTCTAGTTTCAAAATCATGCAAAGGTCTGGCTTCGTTATCGAATTTGACACAGCTAGACTTGAGTTACTGCAATCTGATGGATGGAGCAATTCTCAATGATCTTAGCAGCCTAATCTCCTTAAGATTGTTAGATTTAAGTGGTAATTGCTTTGTGCGATTATCTGAAAGTATCTCTCAACTCTCAAAGCTCGAGACTCTGACCTTGAGTCATTGCAGACAGCTTCAATTGCTGCCCAAGAAGCTTCCGTTAAGTCTTGAGCACGTATGTCTTCCCGGTCAAATTTTTTTTGCCATACATCTTAAAGATGAAATTCCAGAGTGGTTCAGCACTGTCTCTACAAGAAATCACATATCAATCTCGATACCTCAAAATCTGGCTAATGATGACAAGTGGAAGGGATTTGCCACGTGTGTTATATTCTCAGTCAAGGGGCATACTGCTCTCTCCCTTATTGAATCGGATCCTGATTTTTCTAATTACTCGTATCAAATCACATTGGAAACTGATGTTGTGCGTCTGAAACCAGATGTGTGGGAACTGGAATTAGGCCCCTTTGGATTAAGGCTATATGTGATTGCTGTTTTCTCACGAAACTACGACGATTCTGCATGGTGTTTGGATGAGATTGCCAAGGTCGCCGATTGCATGAAAGCCATGGGACAAAAAGTCCTCCCAGTGTTCTATGATGTTGATCCAAATGAGAAATTCCAAAAATGGAAAAATGGGCTGCAGCTCGGGTTTTCGCCGGAAATCGCCTCCGATTTTCGTTTAGCTATATCTTCCTCCTCCGGCCACCAATCTTCACCAAACTTCTTCCATTTGTTTCGTATGGTCCTTGCGCACCTATCCGTGGCAGCCTTGCACGGCGGCGCGGCCTATAGCGGCGGCGGCAAGCTTGTTCCGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

338

Amino Acids

37.59

Weight (kDa)

6.5

Isoelectric Point (pI)

36.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 54 - 116 1.4e-06 Leucine-rich repeat region
C-JID PF20160 138 - 193 8.5e-08 C-JID domain
TIR PF01582 227 - 271 1.8e-11 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 948
AccII CGCG 1 cut(s) 980
AciI CCGC 5 cut(s) 975, 980, 990, 993, 996
AclWI GGATC 3 cut(s) 563, 576, 794
AcoI YGGCCR 1 cut(s) 898
AcsI RAATTY 4 cut(s) 148, 387, 417, 812
AcvI CACGTG 1 cut(s) 516
AfiI CCNNNNNNNGG 1 cut(s) 958
AflII CTTAAG 1 cut(s) 226
AflIII ACRYGT 1 cut(s) 515
AgsI TTSAA 4 cut(s) 62, 117, 332, 563
AhdI GACNNNNNGTC 2 cut(s) 312, 773
AluBI AGCT 7 cut(s) 160, 295, 328, 349, 841, 881, 1003
AluI AGCT 7 cut(s) 160, 295, 328, 349, 841, 881, 1003
Alw21I GWGCWC 1 cut(s) 369
Alw26I GTCTC 2 cut(s) 293, 445
AlwI GGATC 3 cut(s) 563, 576, 794
Ama87I CYCGRG 2 cut(s) 296, 842
AoxI GGCC 3 cut(s) 655, 898, 981
ApeKI GCWGC 5 cut(s) 213, 337, 835, 838, 962
ApoI RAATTY 4 cut(s) 148, 387, 417, 812
AspLEI GCGC 2 cut(s) 949, 980
AspS9I GGNCC 2 cut(s) 656, 940
AsuC2I CCSGG 1 cut(s) 381
AsuHPI GGTGA 1 cut(s) 903
AvaI CYCGRG 2 cut(s) 296, 842
AvaII GGWCC 1 cut(s) 940
BamHI GGATCC 1 cut(s) 568
BbrPI CACGTG 1 cut(s) 516
BbsI GAAGAC 1 cut(s) 368
Bbv12I GWGCWC 1 cut(s) 369
BbvI GCAGC 5 cut(s) 225, 324, 822, 850, 974
BccI CCATC 2 cut(s) 178, 182
BceAI ACGGC 1 cut(s) 988
BcgI CGANNNNNNTGC 2 cut(s) 695, 729
BcnI CCSGG 1 cut(s) 381
BcoDI GTCTC 2 cut(s) 293, 445
BfaI CTAG 2 cut(s) 110, 161
BfmI CTRYAG 2 cut(s) 836, 985
BfrI CTTAAG 1 cut(s) 226
Bme1390I CCNGG 1 cut(s) 381
Bme18I GGWCC 1 cut(s) 940
BmeRI GACNNNNNGTC 2 cut(s) 312, 773
BmeT110I CYCGRG 2 cut(s) 296, 842
BmgT120I GGNCC 2 cut(s) 656, 940
BmiI GGNNCC 2 cut(s) 570, 658
BmrFI CCNGG 1 cut(s) 381
BmrI ACTGGG 1 cut(s) 776
BmsI GCATC 1 cut(s) 6
BmuI ACTGGG 1 cut(s) 776
BpiI GAAGAC 1 cut(s) 368
BplI GAGNNNNNCTC 2 cut(s) 183, 215
BpmI CTGGAG 1 cut(s) 32
BpuEI CTTGAG 3 cut(s) 187, 331, 383
BpuMI CCSGG 1 cut(s) 381
BsaAI YACGTR 2 cut(s) 370, 516
BsaBI GATNNNNATC 1 cut(s) 798
BsaJI CCNNGG 4 cut(s) 94, 738, 762, 957
Bsc4I CCNNNNNNNGG 1 cut(s) 958
Bse1I ACTGG 2 cut(s) 651, 782
Bse3DI GCAATG 1 cut(s) 316
Bse8I GATNNNNATC 1 cut(s) 798
BseDI CCNNGG 4 cut(s) 94, 738, 762, 957
BseGI GGATG 3 cut(s) 104, 193, 733
BseJI GATNNNNATC 1 cut(s) 798
BseLI CCNNNNNNNGG 1 cut(s) 958
BseMI GCAATG 1 cut(s) 316
BseMII CTCAG 1 cut(s) 543
BseNI ACTGG 2 cut(s) 651, 782
BseRI GAGGAG 1 cut(s) 883
BseXI GCAGC 5 cut(s) 225, 324, 822, 850, 974
Bsh1236I CGCG 1 cut(s) 980
BshFI GGCC 3 cut(s) 657, 900, 983
BsiHKAI GWGCWC 1 cut(s) 369
BsiHKCI CYCGRG 2 cut(s) 296, 842
BsiSI CCGG 3 cut(s) 381, 854, 897
BslFI GGGAC 1 cut(s) 780
BslI CCNNNNNNNGG 1 cut(s) 958
BsmAI GTCTC 2 cut(s) 293, 445
BsmFI GGGAC 1 cut(s) 780
BsnI GGCC 3 cut(s) 657, 900, 983
BsoBI CYCGRG 2 cut(s) 296, 842
Bsp1286I GDGCHC 1 cut(s) 369
Bsp143I GATC 3 cut(s) 205, 568, 799
Bsp19I CCATGG 1 cut(s) 762
BspACI CCGC 5 cut(s) 975, 980, 990, 993, 996
BspANI GGCC 3 cut(s) 657, 900, 983
BspCNI CTCAG 1 cut(s) 542
BspFNI CGCG 1 cut(s) 980
BspLI GGNNCC 2 cut(s) 570, 658
BspMAI CTGCAG 1 cut(s) 840
BspPI GGATC 3 cut(s) 563, 576, 794
BspTI CTTAAG 1 cut(s) 226
BsrDI GCAATG 1 cut(s) 316
BsrI ACTGG 2 cut(s) 651, 782
BssECI CCNNGG 4 cut(s) 94, 738, 762, 957
BssMI GATC 3 cut(s) 205, 568, 799
BssT1I CCWWGG 2 cut(s) 738, 762
Bst4CI ACNGT 2 cut(s) 81, 439
BstAFI CTTAAG 1 cut(s) 226
BstBAI YACGTR 2 cut(s) 370, 516
BstC8I GCNNGC 1 cut(s) 1001
BstDEI CTNAG 2 cut(s) 209, 529
BstDSI CCRYGG 2 cut(s) 762, 957
BstF5I GGATG 3 cut(s) 104, 193, 733
BstFNI CGCG 1 cut(s) 980
BstHHI GCGC 2 cut(s) 949, 980
BstKTI GATC 3 cut(s) 208, 571, 802
BstMAI GTCTC 2 cut(s) 293, 445
BstMBI GATC 3 cut(s) 205, 568, 799
BstMWI GCNNNNNNNGC 4 cut(s) 334, 346, 743, 996
BstSCI CCNGG 1 cut(s) 379
BstSFI CTRYAG 2 cut(s) 836, 985
BstUI CGCG 1 cut(s) 980
BstV1I GCAGC 5 cut(s) 225, 324, 822, 850, 974
BstV2I GAAGAC 1 cut(s) 368
BstX2I RGATCY 1 cut(s) 568
BstXI CCANNNNNNTGG 1 cut(s) 639
BstYI RGATCY 1 cut(s) 568
BsuRI GGCC 3 cut(s) 657, 900, 983
BtgI CCRYGG 2 cut(s) 762, 957
BtsCI GGATG 3 cut(s) 104, 193, 733
BtsIMutI CAGTG 2 cut(s) 435, 789
Cac8I GCNNGC 1 cut(s) 1001
CfoI GCGC 2 cut(s) 949, 980
Cfr13I GGNCC 2 cut(s) 656, 940
CseI GACGC 1 cut(s) 611
CviAII CATG 5 cut(s) 67, 123, 717, 754, 763
DdeI CTNAG 2 cut(s) 209, 529
DpnI GATC 3 cut(s) 207, 570, 801
DpnII GATC 3 cut(s) 205, 568, 799
DriI GACNNNNNGTC 2 cut(s) 312, 773
EaeI YGGCCR 1 cut(s) 898
Eam1105I GACNNNNNGTC 2 cut(s) 312, 773
Eco130I CCWWGG 2 cut(s) 738, 762
Eco47I GGWCC 1 cut(s) 940
Eco72I CACGTG 1 cut(s) 516
Eco88I CYCGRG 2 cut(s) 296, 842
EcoO109I RGGNCCY 1 cut(s) 656
EcoT14I CCWWGG 2 cut(s) 738, 762
ErhI CCWWGG 2 cut(s) 738, 762
FaeI CATG 5 cut(s) 70, 126, 720, 757, 766
FaqI GGGAC 1 cut(s) 780
FatI CATG 5 cut(s) 66, 122, 716, 753, 762
FokI GGATG 3 cut(s) 111, 200, 740
FspBI CTAG 2 cut(s) 110, 161
FspI TGCGCA 1 cut(s) 948
GlaI GCGC 2 cut(s) 948, 979
GsuI CTGGAG 1 cut(s) 32
HaeIII GGCC 3 cut(s) 657, 900, 983
HapII CCGG 3 cut(s) 381, 854, 897
HgaI GACGC 1 cut(s) 611
HhaI GCGC 2 cut(s) 949, 980
Hin1II CATG 5 cut(s) 70, 126, 720, 757, 766
Hin6I GCGC 2 cut(s) 947, 978
HinP1I GCGC 2 cut(s) 947, 978
HindIII AAGCTT 2 cut(s) 347, 1001
HinfI GANTC 5 cut(s) 73, 301, 313, 563, 710
HpaII CCGG 3 cut(s) 381, 854, 897
HphI GGTGA 1 cut(s) 903
Hpy166II GTNNAC 1 cut(s) 104
Hpy188I TCNGA 7 cut(s) 183, 271, 306, 568, 627, 868, 1011
Hpy188III TCNNGA 7 cut(s) 11, 298, 362, 422, 466, 572, 696
Hpy8I GTNNAC 1 cut(s) 104
Hpy99I CGWCG 1 cut(s) 710
HpyAV CCTTC 2 cut(s) 56, 496
HpyCH4III ACNGT 2 cut(s) 81, 439
HpyCH4IV ACGT 3 cut(s) 106, 369, 515
HpyCH4V TGCA 7 cut(s) 126, 177, 321, 716, 753, 838, 970
HpyF10VI GCNNNNNNNGC 4 cut(s) 334, 346, 743, 996
HpyF3I CTNAG 2 cut(s) 209, 529
HpySE526I ACGT 3 cut(s) 106, 369, 515
Hsp92II CATG 5 cut(s) 70, 126, 720, 757, 766
HspAI GCGC 2 cut(s) 947, 978
Kzo9I GATC 3 cut(s) 205, 568, 799
LmnI GCTCC 1 cut(s) 191
Lsp1109I GCAGC 5 cut(s) 225, 324, 822, 850, 974
LweI GCATC 1 cut(s) 6
MaeI CTAG 2 cut(s) 110, 161
MaeII ACGT 3 cut(s) 106, 369, 515
MaeIII GTNAC 1 cut(s) 170
MalI GATC 3 cut(s) 207, 570, 801
MboI GATC 3 cut(s) 205, 568, 799
MboII GAAGA 5 cut(s) 82, 368, 879, 900, 913
MfeI CAATTG 1 cut(s) 332
MflI RGATCY 1 cut(s) 568
MhlI GDGCHC 1 cut(s) 369
MluCI AATT 9 cut(s) 148, 195, 250, 332, 387, 417, 583, 650, 812
MlyI GAGTC 2 cut(s) 295, 322
MnlI CCTC 6 cut(s) 89, 483, 788, 874, 901, 904
MseI TTAA 5 cut(s) 227, 242, 356, 408, 668
MspCI CTTAAG 1 cut(s) 226
MspI CCGG 3 cut(s) 381, 854, 897
MspR9I CCNGG 1 cut(s) 381
MunI CAATTG 1 cut(s) 332
MvnI CGCG 1 cut(s) 980
MwoI GCNNNNNNNGC 4 cut(s) 334, 346, 743, 996
NciI CCSGG 1 cut(s) 381
NcoI CCATGG 1 cut(s) 762
NdeII GATC 3 cut(s) 205, 568, 799
NlaIII CATG 5 cut(s) 70, 126, 720, 757, 766
NlaIV GGNNCC 2 cut(s) 570, 658
NmeAIII GCCGAG 1 cut(s) 119
NsbI TGCGCA 1 cut(s) 948
PaeR7I CTCGAG 1 cut(s) 296
PfeI GAWTC 3 cut(s) 73, 563, 710
PleI GAGTC 2 cut(s) 295, 321
PmaCI CACGTG 1 cut(s) 516
PmlI CACGTG 1 cut(s) 516
PpsI GAGTC 2 cut(s) 295, 321
Ppu21I YACGTR 2 cut(s) 370, 516
PspCI CACGTG 1 cut(s) 516
PspN4I GGNNCC 2 cut(s) 570, 658
PspPI GGNCC 2 cut(s) 656, 940
PstI CTGCAG 1 cut(s) 840
PsuI RGATCY 1 cut(s) 568
SaqAI TTAA 5 cut(s) 227, 242, 356, 408, 668
Sau3AI GATC 3 cut(s) 205, 568, 799
Sau96I GGNCC 2 cut(s) 656, 940
SchI GAGTC 2 cut(s) 295, 322
ScrFI CCNGG 1 cut(s) 381
SduI GDGCHC 1 cut(s) 369
SfaNI GCATC 1 cut(s) 6
SfcI CTRYAG 2 cut(s) 836, 985
Sfr274I CTCGAG 1 cut(s) 296
SinI GGWCC 1 cut(s) 940
SlaI CTCGAG 1 cut(s) 296
SmlI CTYRAG 5 cut(s) 166, 226, 296, 310, 362
SmoI CTYRAG 5 cut(s) 166, 226, 296, 310, 362
Sse9I AATT 9 cut(s) 148, 195, 250, 332, 387, 417, 583, 650, 812
SsiI CCGC 5 cut(s) 975, 980, 990, 993, 996
SspMI CTAG 2 cut(s) 110, 161
StyD4I CCNGG 1 cut(s) 379
StyI CCWWGG 2 cut(s) 738, 762
TaaI ACNGT 2 cut(s) 81, 439
TaiI ACGT 3 cut(s) 109, 372, 518
TaqI TCGA 3 cut(s) 146, 297, 467
TasI AATT 9 cut(s) 148, 195, 250, 332, 387, 417, 583, 650, 812
TauI GCSGC 5 cut(s) 978, 983, 993, 996, 999
TfiI GAWTC 3 cut(s) 73, 563, 710
Tru1I TTAA 5 cut(s) 227, 242, 356, 408, 668
Tru9I TTAA 5 cut(s) 227, 242, 356, 408, 668
TscAI CASTG 2 cut(s) 442, 789
TseI GCWGC 5 cut(s) 213, 337, 835, 838, 962
TspDTI ATGAA 4 cut(s) 17, 83, 429, 770
TspGWI ACGGA 2 cut(s) 342, 946
TspRI CASTG 2 cut(s) 442, 789
Vha464I CTTAAG 1 cut(s) 226
VpaK11BI GGWCC 1 cut(s) 940
XapI RAATTY 4 cut(s) 148, 387, 417, 812
XhoI CTCGAG 1 cut(s) 296
XspI CTAG 2 cut(s) 110, 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.