MD17G1192900.v1.1

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000129
Physical Location & Seq
Forward (+)
23117831 .. 23124727
6897 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1192900.v1.1.491

Sequence Viewer

Length: 4986 bp
ATGATCGGCCAAACAGATATTCCTTCTTCTTCTTATTGGTCTCCTCCGACCCCTCAATGGACATACGATGTGTTCCTCAGTTTCAGAAGCGAAGACACCCATAGAAATTTTACAGACCATCTGTATACTTCTCTGAATCAGAAGGGAGTATTTGCATTCAGAGATGCTGAACAACTTGAGAGGGAAAAACCCATCTCGCCAGAACTCTTGAAAGCAATTGAAGAATCAAGATATGTAATTGTCGTTCTTTCAAGAAACTATGCCTACTCAACATGTTGCTTGGATGAGCTTGCAAAGAGTGTTGAATGCATGAAAGTGATGGGGCAGGCATTACTCCCAGTTTTCTATGATGTGGATCCATCTGAGGTACAAAAACAAATGGGGAATTTCGGGAAAGCCTTCTCCAAGCATGAAGAAACCTTTAAGGATAATACAGAGAAGGTGCAAAAGTGGAGAGATGCACTTACTGAAGTGGCTGATCTCTATGGATGGCATGTACAAGATGGGTATGAATCGAAAATTATTCAAGATATTGTGAGATATATTTTCACAAAAGTAAATCAAACAATTTCAAGTATATATACGGATTTAGTTGGGATGGATTCTCGCGTAAACAAAGTGATTTCTTGCTTAGACATGGCGTTGAACAATGTATGTATGTTAGGGATGTGGGGGATGGGGGGGATTGGTAAGACAACCACTGCAGAAGTAGTTTTTGATAGGATACGTTCTCAGTTTGAAGCTTACAGCTTTCTTGCCAATGTTAGAGAGGTAACCCAGAAACAAGGTCTAGTCCATTTACAAAAGCAACTTCTTTCGGATATCCTGTTCGAGAGTAATGTAGATGTACATAATGTCCATATGGGGATCAGTAAAATAAGGCAGAGACTGTGTAATAGAATGGTTCTTATCATTCTTGATGATGTGGATCAATTAGAACAATTGGAAGCATTGTGTGATCATAGTTGGTTTGGTTCAGGGAGTAGAATTATAATAACATCGAGGGATGAACATTTGTTACGCACATTTGGAGTGGATATAATGTATAAGGTTAAGGCATTAACTGATGCTGAAGCTCTTCAGCTCTTTTGTCGGAAAGCATTCAAGAAAGGCCAGGTCAGTGAAGACTTTCTCAAGCTGGCAAACAATGTTGTAGAATACGCTAATGGCCTCCCATTAGCTATTGAAGTTTTGGGTTCTTTCCTCCTTGGTAGAAGTGTAGAAGTATGGTCAAGTGCTTTGGATAGCCTTAAAGAAAACCCTGACAAAAGAATAACTGAGGTGCTTAAAGTAAGTTTTGATGCTTTAGAAGACATAGAAAAGAAGGTATTTTTGGACATTGCATGTTTCTTTAAAGGGGAGGAGAAAGATCGTGTTACAAGAATACTGGAAAGTAGTTGTGGCTATCGTCCAGACATTGATGTAGCAGTTCTCATAGAAAAATCTTTACTAACTCTATTTGGAAGAAAATTGTGGATGCATGATTTGATACAAGAATTAGGTTGGGAGATTGTTCGTCGTGAATGCAGTGAAGATCCGGGCAAACGTAGCAGGTTGTGGCTTCCCAAGGATATAATCCATGTACTTGTAAACAATGAGGGAACACTTGCGGTGGAAGGTATATTCCTGAATCTACCAAAACAAGAGGTGGTTCACGTAAATGTTGACCCCTTCTCAAAGATGTGCAAGCTAAGATTGTTAAAAATTAGTAATGTGTTTTTTTCTGGATCCATCAAATACCTTTCTAACGAGTTACAACTGCTGGAATGGCATGCATTTCCTTTTAGTTCTTTGCCATCAAACTTTCAATCAGATAAGCTTGTGGAACTCCACATGCATTCAAGCCTTATCAAACAACTATGGAGGGGAAATAAACGCTGGAGCATGCTAAAGTCTATTGATCTAAGTGATTCCTTGTACTTGATTGCGACCCCAAACTTCATTGAGGTTCCAAATCTTGAGAAGTTGGTGCTTCAAGGTTGCACAAGATTGGTTGAGGTTCACCCTTCCCTTGGATTGCTCAAAAACCTTATCTTGTTGAATATGAGAAACTGCAAATCTATTGAGAGCCTTCCACCTTTCATCAATTTGGAATCTCTTAGAACTTTAACTCTCTCTGGTTCTTCAAGACTCAAGAGGTTTCCTCAAATTGAAGGAAATATGAAAAGCTTGTTGGAGCTTCATCTAGATGGAACTGCCATTGAAGAATTACCACCATCAATTCAACGTTTGACTGGTCTTACATTGATGAATATGGGAGGCTGCAAAAACCTTTTCCGTCTTCGGAATACCATTCAATATTTGACATCTCTTGAAACTCTTATTCTAACAGGTTGCTCAGAACTTGATGACATACCTGAGGATTTGAATTGTGTGGAATGTCTTGAGGAGCTTGATATAAGTGGAACTGCTATAAGAGCATCATCATCCATTGCGGCTATGAAAAATCTAAGGACTTTATCCTTCCAAGGATGTAAAGATCTACCTTCAAAATCGTGGCATTCTCTCTTTAATTGTTGGTGGTGGGGTAGAAAGGGCAATGTTCCTGTGAGTTTGTTGTTGCCTACTTCGTTGTCAGGTTTAACTTCCCTGACGAGCCTAAACTTAAGTGATTGCAATCTGATAGATGGAGCAATACCTGATGATCTTGGCACCTTAATCTCCTTAAGGACATTAAACTTAAGTGCTAATAACTTTGTTTGCTTACCTGAAAGCATCGGCCAGCTATCTAAGCTTGAGTCTCTCAACTTGATGAATTGTAGTAGGCTTCTGTCACTGCCTAAGAAGCTTCCATTAAGTGTTCGACACGTCCCTGCAGATGATTGTACATCACTGATAGATTTCCCTGATCAATTCAAAGTATGGACCTCAGCTGTTTCTGGAATGACTACAATCAGTGCCCTCATTTCATCCAAGCATCAAGAATCTTCTACTTCATCACCACTAGACCAAAAATACACAAAGACATGGACTTCATCAACTGTCCCAGGACGTAGTTTCTTTAGTTTTGGCCCCGAATCAGATAAGCATCAACAATGGAGTCCAGTTCATGATCTTTCACTGCCAAATCAAGTTCTGCAAAAAGACATTGAACTATATGATTATAAGTCACATTCCATAAAGGCTGCTTGTTATCAAAATGAAATTCCAGAGTGGTTCAGCGGCAGAGCTACGGGAGATTTCATATCGATCCCGGTGCCTCCAGATTTGAAAAATGAGAAGAAGTGGATGGGGGTTGCTATTGTTTTCTTGGTGAAGGGAAAACCTGGTGTCGCCCATGTTAAATCTGATTCAGAGGCTTCTGATTACTTCTACAAATGCAGAGTGGAAACTGAGGAATTTCAACTGGAACCATTCCTTCTTGACTGGAGACATCTCAACTCAATTGAATCAAGTTCTGAGTTACTTTGTTTTTTTTATGTACCTTGTGTGTGCTTTCCAAGGATGTTAAATGAATCGTTCGTGATTGGGACTTTATTTGAAACCAACAACCGGTGCATGGAGGTCCAGAAATGTGGGATTCGTCTTGTTTACGAGCAAGATGTTGAACAGTTTATCCAAACATTCATACCACAAAGTTTTGGTTTTGGAGGGCGGCAGCAACAACAAATCCATTTGCTGACCAAACAAAATCAACAACTTATCCCTTCTGACCCTTCCAATCTGAAAGTTGGAAAAGCTACATCAGAATCTGGATGGTTTAATCTAGATAATGACATTTTGAGATGGGAGAAATTTATTCCAACGTGTGAAGAAGCTTCCACAGTTTTGTTGAGAAGGAACATCGAGTCTGTTCTTCCAAGATACCTGGAGGGACTAAACCATAGGTGGCAAGACTATGGATTTCCTTTAAGTGGAAGCCCAGCATGGTTTAATACTGAAGTTGGGACGTCTGTGACAATCGAGCTGCCTCAGAATCTGCACAAGTGTAAGAAGTGGATGGGTTTTTCTCTATGCGCATCACTTGCAGTAGAACACCAGCACATCGTAAAAGAGGATCAGTATCATGGTTCTTGTCGACTCGAAATGGGTAAACATTATATGAATCTGTCCCTAAACTTAGAGTCTCTGATTTCTGATAAACATCACCTTTTGATTGCTTACATTCCACGGTCGATGATTCCCGAACTGTTCACTCAAACATCACCAAGGCAAATCTGCCTTTCCTTTATTACCTACAGACCTGCTTTAAAGGTTAAAATCTGTGGGCTCCGTATTTTGTACAAGCAAGATTTACAAGGATTTGTCCAGACAATCACACAGTGCATATTAGGCAGTTCAGACACCCTCTTTGGATATTATAATGATTTGGTGGTAAAACATTGGATAAACTTAATTCTACTGCAAAGTTGTAAAGTCCTTACAGAGGAAAAACAGAGTCCCCGAGAATGTGATACTCGATTACAGCAAAGAAAGTTCCAGCTGATCTCTCAAAATTTCAGAAACCGGGTCTGGTCTGACAATCACTTTGCATTTAATTTTGCTGGAATTGAAATTCCGAAGTGGTTCTTGAATCCAAATAAAGGCGACTGTGCACAGATTGATATGCCTCCAAATTTGTTCGATGATGGTAATTGGTTAGGAATTGCTGTATCTGGTTCTGTATCGATCCATGAACATCCAAATATCATTCAGGACACTCCCGACTCAGAATTTGATCGTGAGGTCATTTGTGATCTGAAACCCGGGGTTGAATTCGACAATAGTCTCCGTTTGGCATTCGGTACGGAGAAAGAGATATGGTTGCATGCACGTGATTTGACTTGGTTTGTATATGTATCACGTGCTCTATTTCCTGAATCGTGGAATAAATGCCATATAGTCAGTGCAACTTTAGGGTCCAACGGCCCAGGCTTAGGATTGCATGAATGTGCCATTCGTCTGGTGTTTAAGGAGGATGTTGAAGAGCTTGTACAAACGCTGACGCTCTGCGAGTTATCTTCAAACTGCGGTCATTGCAAGCAAGCTGCAGAATATGGTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1662

Amino Acids

188.98

Weight (kDa)

5.89

Isoelectric Point (pI)

48.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 22 - 196 1.6e-50 TIR domain
TIR_2 PF13676 24 - 121 1.9e-12 TIR domain
NB-ARC PF00931 202 - 370 1.7e-19 NB-ARC domain
NACHT PF05729 225 - 367 7.6e-06 NACHT domain
WHD_ROQ1 PF23282 439 - 507 4.7e-15 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 666 - 829 2.7e-11 Leucine-rich repeat region
LRR_13 PF23286 697 - 783 2.2e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 856 - 928 1.1e-06 Leucine-rich repeat region
C-JID PF20160 1046 - 1183 2.1e-06 C-JID domain
C-JID PF20160 1287 - 1412 1.4e-08 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 992, 3105, 4302
AatII GACGTC 1 cut(s) 3893
Acc16I TGCGCA 1 cut(s) 3958
Acc36I ACCTGC 2 cut(s) 1542, 4192
AccB1I GGYRCC 2 cut(s) 2651, 3196
AccI GTMKAC 2 cut(s) 125, 4018
AccII CGCG 1 cut(s) 609
AciI CCGC 5 cut(s) 1610, 2435, 3162, 3595, 4950
AclI AACGTT 1 cut(s) 2227
AcoI YGGCCR 2 cut(s) 7, 2719
AcuI CTGAAG 4 cut(s) 489, 1064, 1092, 3900
AcvI CACGTG 2 cut(s) 4754, 4784
AcyI GRCGYC 1 cut(s) 3890
AdeI CACNNNGTG 1 cut(s) 4263
AfiI CCNNNNNNNGG 8 cut(s) 57, 2012, 3492, 3499, 3854, 4366, 4523, 4856
AflII CTTAAG 3 cut(s) 2605, 2665, 2680
AflIII ACRYGT 3 cut(s) 272, 2806, 3746
AgeI ACCGGT 1 cut(s) 3492
AjiI CACGTC 1 cut(s) 2809
AjnI CCWGG 5 cut(s) 1113, 2986, 3265, 3807, 4849
AjuI GAANNNNNNNTTGG 5 cut(s) 35, 1316, 1348, 2156, 2188
AloI GAACNNNNNNTCC 4 cut(s) 812, 844, 3540, 3572
Alw21I GWGCWC 2 cut(s) 4537, 4789
Alw26I GTCTC 6 cut(s) 45, 880, 2745, 3364, 4071, 4712
Alw44I GTGCAC 1 cut(s) 4533
AlwNI CAGNNNCTG 3 cut(s) 889, 3692, 3919
Ama87I CYCGRG 2 cut(s) 4383, 4685
AoxI GGCC 6 cut(s) 7, 1111, 1168, 2719, 3010, 4846
ApaLI GTGCAC 1 cut(s) 4533
ApeKI GCWGC 5 cut(s) 2262, 3125, 3598, 3907, 4967
ArsI GACNNNNNNTTYG 6 cut(s) 4274, 4306, 4451, 4483, 4637, 4669
AsiGI ACCGGT 1 cut(s) 3492
Asp700I GAANNNNTTC 5 cut(s) 398, 1077, 1100, 1128, 4505
AspLEI GCGC 1 cut(s) 3959
AspS9I GGNCC 5 cut(s) 2865, 3011, 3505, 4839, 4847
AsuC2I CCSGG 5 cut(s) 1539, 3194, 4448, 4686, 4687
AsuHPI GGTGA 5 cut(s) 1994, 2931, 3265, 4079, 4137
AvaI CYCGRG 2 cut(s) 4383, 4685
AvaII GGWCC 3 cut(s) 2865, 3505, 4839
AxyI CCTNAGG 1 cut(s) 2358
BaeGI GKGCMC 2 cut(s) 2902, 4537
BamHI GGATCC 2 cut(s) 355, 1727
BanI GGYRCC 2 cut(s) 2651, 3196
BanII GRGCYC 1 cut(s) 4212
BarI GAAGNNNNNNTAC 2 cut(s) 4896, 4928
BbrPI CACGTG 2 cut(s) 4754, 4784
BbsI GAAGAC 4 cut(s) 99, 1131, 1317, 2273
Bbv12I GWGCWC 2 cut(s) 4537, 4789
BbvCI CCTCAGC 1 cut(s) 2869
BbvI GCAGC 5 cut(s) 2249, 3112, 3610, 3894, 4954
BceAI ACGGC 1 cut(s) 4861
BcgI CGANNNNNNTGC 2 cut(s) 3178, 3212
BciT130I CCWGG 5 cut(s) 1115, 2988, 3267, 3809, 4851
BciVI GTATCC 1 cut(s) 717
BclI TGATCA 2 cut(s) 958, 2848
BcnI CCSGG 5 cut(s) 1539, 3194, 4448, 4686, 4687
BcoDI GTCTC 6 cut(s) 45, 880, 2745, 3364, 4071, 4712
BfaI CTAG 4 cut(s) 791, 2186, 2945, 3707
BfmI CTRYAG 4 cut(s) 702, 2814, 4177, 4968
BfrI CTTAAG 3 cut(s) 2605, 2665, 2680
BfuAI ACCTGC 2 cut(s) 1542, 4192
BfuI GTATCC 1 cut(s) 717
BglII AGATCT 1 cut(s) 2479
BisI GCNGC 8 cut(s) 2263, 2436, 3126, 3163, 3596, 3599, 3908, 4968
BlsI GCNGC 8 cut(s) 2264, 2437, 3127, 3164, 3597, 3600, 3909, 4969
Bme18I GGWCC 3 cut(s) 2865, 3505, 4839
BmeT110I CYCGRG 2 cut(s) 4383, 4685
BmgBI CACGTC 1 cut(s) 2809
BmgT120I GGNCC 5 cut(s) 2865, 3011, 3505, 4839, 4847
BmiI GGNNCC 9 cut(s) 357, 1729, 1950, 2653, 3013, 3198, 3351, 4211, 4840
BmrI ACTGGG 1 cut(s) 332
BmuI ACTGGG 1 cut(s) 332
BoxI GACNNNNGTC 1 cut(s) 4704
BpiI GAAGAC 4 cut(s) 99, 1131, 1317, 2273
BplI GAGNNNNNCTC 2 cut(s) 2128, 2160
BpmI CTGGAG 4 cut(s) 1900, 3186, 3388, 3830
Bpu10I CCTNAGC 2 cut(s) 2869, 4855
BpuEI CTTGAG 6 cut(s) 197, 1118, 1979, 2117, 2405, 2756
BpuMI CCSGG 5 cut(s) 1539, 3194, 4448, 4686, 4687
Bsa29I ATCGAT 2 cut(s) 3188, 4607
BsaAI YACGTR 3 cut(s) 1657, 4754, 4784
BsaBI GATNNNNATC 6 cut(s) 354, 927, 2616, 3027, 4002, 4083
BsaHI GRCGYC 1 cut(s) 3890
BsaI GGTCTC 1 cut(s) 45
BsaWI WCCGGW 1 cut(s) 3492
Bsc4I CCNNNNNNNGG 8 cut(s) 57, 2012, 3492, 3499, 3854, 4366, 4523, 4856
Bse118I RCCGGY 1 cut(s) 3492
Bse1I ACTGG 6 cut(s) 338, 1392, 2239, 3044, 3351, 3371
Bse21I CCTNAGG 1 cut(s) 2358
Bse3DI GCAATG 4 cut(s) 1338, 2430, 2545, 4954
Bse8I GATNNNNATC 6 cut(s) 354, 927, 2616, 3027, 4002, 4083
BseBI CCWGG 5 cut(s) 1115, 2988, 3267, 3809, 4851
BseCI ATCGAT 2 cut(s) 3188, 4607
BseJI GATNNNNATC 6 cut(s) 354, 927, 2616, 3027, 4002, 4083
BseLI CCNNNNNNNGG 8 cut(s) 57, 2012, 3492, 3499, 3854, 4366, 4523, 4856
BseMI GCAATG 4 cut(s) 1338, 2430, 2545, 4954
BseNI ACTGG 6 cut(s) 338, 1392, 2239, 3044, 3351, 3371
BseRI GAGGAG 3 cut(s) 33, 1376, 2402
BseSI GKGCMC 2 cut(s) 2902, 4537
BseXI GCAGC 5 cut(s) 2249, 3112, 3610, 3894, 4954
BseYI CCCAGC 1 cut(s) 3862
BsgI GTGCAG 1 cut(s) 3905
Bsh1236I CGCG 1 cut(s) 609
Bsh1285I CGRYCG 1 cut(s) 4115
BshFI GGCC 6 cut(s) 9, 1113, 1170, 2721, 3012, 4848
BshNI GGYRCC 2 cut(s) 2651, 3196
BshTI ACCGGT 1 cut(s) 3492
BshVI ATCGAT 2 cut(s) 3188, 4607
BsiEI CGRYCG 1 cut(s) 4115
BsiHKAI GWGCWC 2 cut(s) 4537, 4789
BsiHKCI CYCGRG 2 cut(s) 4383, 4685
BsiSI CCGG 5 cut(s) 1538, 3194, 3493, 4447, 4686
BslFI GGGAC 7 cut(s) 2795, 2969, 3484, 3828, 3901, 4036, 4365
BslI CCNNNNNNNGG 8 cut(s) 57, 2012, 3492, 3499, 3854, 4366, 4523, 4856
BsmAI GTCTC 6 cut(s) 45, 880, 2745, 3364, 4071, 4712
BsmFI GGGAC 7 cut(s) 2795, 2969, 3484, 3828, 3901, 4036, 4365
BsmI GAATGC 7 cut(s) 155, 311, 1100, 1529, 1837, 2500, 4718
BsnI GGCC 6 cut(s) 9, 1113, 1170, 2721, 3012, 4848
Bso31I GGTCTC 1 cut(s) 45
BsoBI CYCGRG 2 cut(s) 4383, 4685
Bsp1286I GDGCHC 4 cut(s) 2902, 4212, 4537, 4789
Bsp1407I TGTACA 5 cut(s) 496, 847, 2825, 4221, 4912
BspACI CCGC 5 cut(s) 1610, 2435, 3162, 3595, 4950
BspANI GGCC 6 cut(s) 9, 1113, 1170, 2721, 3012, 4848
BspDI ATCGAT 2 cut(s) 3188, 4607
BspFNI CGCG 1 cut(s) 609
BspHI TCATGA 1 cut(s) 3049
BspLI GGNNCC 9 cut(s) 357, 1729, 1950, 2653, 3013, 3198, 3351, 4211, 4840
BspMAI CTGCAG 3 cut(s) 706, 2818, 4972
BspMI ACCTGC 2 cut(s) 1542, 4192
BspQI GCTCTTC 2 cut(s) 1083, 4899
BspT107I GGYRCC 2 cut(s) 2651, 3196
BspTI CTTAAG 3 cut(s) 2605, 2665, 2680
BspTNI GGTCTC 1 cut(s) 45
BsrDI GCAATG 4 cut(s) 1338, 2430, 2545, 4954
BsrFI RCCGGY 1 cut(s) 3492
BsrGI TGTACA 5 cut(s) 496, 847, 2825, 4221, 4912
BsrI ACTGG 6 cut(s) 338, 1392, 2239, 3044, 3351, 3371
BssAI RCCGGY 1 cut(s) 3492
BssNAI GTATAC 1 cut(s) 126
BssNI GRCGYC 1 cut(s) 3890
BssT1I CCWWGG 6 cut(s) 1207, 1566, 2011, 2467, 3440, 4148
Bst1107I GTATAC 1 cut(s) 126
Bst2UI CCWGG 5 cut(s) 1115, 2988, 3267, 3809, 4851
Bst4CI ACNGT 8 cut(s) 891, 2983, 3552, 3766, 4113, 4131, 4263, 4532
Bst6I CTCTTC 2 cut(s) 1083, 4899
BstACI GRCGYC 1 cut(s) 3890
BstAFI CTTAAG 3 cut(s) 2605, 2665, 2680
BstAPI GCANNNNNTGC 1 cut(s) 3965
BstAUI TGTACA 5 cut(s) 496, 847, 2825, 4221, 4912
BstBAI YACGTR 3 cut(s) 1657, 4754, 4784
BstDSI CCRYGG 1 cut(s) 4109
BstEII GGTNACC 1 cut(s) 772
BstENI CCTNNNNNAGG 1 cut(s) 4364
BstFNI CGCG 1 cut(s) 609
BstHHI GCGC 1 cut(s) 3959
BstMAI GTCTC 6 cut(s) 45, 880, 2745, 3364, 4071, 4712
BstMCI CGRYCG 1 cut(s) 4115
BstMWI GCNNNNNNNGC 8 cut(s) 1548, 1768, 2417, 2731, 2785, 3965, 4272, 4956
BstNI CCWGG 5 cut(s) 1115, 2988, 3267, 3809, 4851
BstNSI RCATGY 7 cut(s) 276, 497, 1347, 1775, 1837, 1888, 4751
BstPAI GACNNNNGTC 1 cut(s) 4704
BstPI GGTNACC 1 cut(s) 772
BstSFI CTRYAG 4 cut(s) 702, 2814, 4177, 4968
BstSLI GKGCMC 2 cut(s) 2902, 4537
BstUI CGCG 1 cut(s) 609
BstV1I GCAGC 5 cut(s) 2249, 3112, 3610, 3894, 4954
BstV2I GAAGAC 4 cut(s) 99, 1131, 1317, 2273
BstX2I RGATCY 4 cut(s) 355, 1534, 1727, 2479
BstXI CCANNNNNNTGG 2 cut(s) 3515, 4882
BstYI RGATCY 4 cut(s) 355, 1534, 1727, 2479
BstZ17I GTATAC 1 cut(s) 126
Bsu15I ATCGAT 2 cut(s) 3188, 4607
Bsu36I CCTNAGG 1 cut(s) 2358
BsuI GTATCC 1 cut(s) 717
BsuRI GGCC 6 cut(s) 9, 1113, 1170, 2721, 3012, 4848
BsuTUI ATCGAT 2 cut(s) 3188, 4607
BtgI CCRYGG 1 cut(s) 4109
BtrI CACGTC 1 cut(s) 2809
BtsI GCAGTG 4 cut(s) 699, 1534, 2774, 3059
BtsIMutI CAGTG 9 cut(s) 699, 1126, 1534, 2774, 2831, 2902, 3059, 4268, 4831
BveI ACCTGC 2 cut(s) 1542, 4192
CaiI CAGNNNCTG 3 cut(s) 889, 3692, 3919
CciI TCATGA 1 cut(s) 3049
CfoI GCGC 1 cut(s) 3959
Cfr10I RCCGGY 1 cut(s) 3492
Cfr13I GGNCC 5 cut(s) 2865, 3011, 3505, 4839, 4847
Cfr9I CCCGGG 1 cut(s) 4685
ClaI ATCGAT 2 cut(s) 3188, 4607
CseI GACGC 1 cut(s) 4933
CsiI ACCWGGT 1 cut(s) 3265
CspAI ACCGGT 1 cut(s) 3492
DraI TTTAAA 2 cut(s) 1354, 4191
DraIII CACNNNGTG 1 cut(s) 4263
EaeI YGGCCR 2 cut(s) 7, 2719
Eam1104I CTCTTC 2 cut(s) 1083, 4899
EarI CTCTTC 2 cut(s) 1083, 4899
Eco130I CCWWGG 6 cut(s) 1207, 1566, 2011, 2467, 3440, 4148
Eco24I GRGCYC 1 cut(s) 4212
Eco31I GGTCTC 1 cut(s) 45
Eco32I GATATC 1 cut(s) 823
Eco47I GGWCC 3 cut(s) 2865, 3505, 4839
Eco57I CTGAAG 4 cut(s) 489, 1064, 1092, 3900
Eco72I CACGTG 2 cut(s) 4754, 4784
Eco81I CCTNAGG 1 cut(s) 2358
Eco88I CYCGRG 2 cut(s) 4383, 4685
Eco91I GGTNACC 1 cut(s) 772
EcoNI CCTNNNNNAGG 1 cut(s) 4364
EcoO65I GGTNACC 1 cut(s) 772
EcoRI GAATTC 1 cut(s) 4694
EcoRII CCWGG 5 cut(s) 1113, 2986, 3265, 3807, 4849
EcoRV GATATC 1 cut(s) 823
EcoT14I CCWWGG 6 cut(s) 1207, 1566, 2011, 2467, 3440, 4148
EcoT22I ATGCAT 4 cut(s) 311, 1482, 1777, 1839
EcoT38I GRGCYC 1 cut(s) 4212
ErhI CCWWGG 6 cut(s) 1207, 1566, 2011, 2467, 3440, 4148
FalI AAGNNNNNCTT 2 cut(s) 4493, 4525
FaqI GGGAC 7 cut(s) 2795, 2969, 3484, 3828, 3901, 4036, 4365
FauNDI CATATG 1 cut(s) 861
FbaI TGATCA 2 cut(s) 958, 2848
FblI GTMKAC 2 cut(s) 125, 4018
Fnu4HI GCNGC 8 cut(s) 2263, 2436, 3126, 3163, 3596, 3599, 3908, 4968
FriOI GRGCYC 1 cut(s) 4212
Fsp4HI GCNGC 8 cut(s) 2263, 2436, 3126, 3163, 3596, 3599, 3908, 4968
FspAI RTGCGCAY 1 cut(s) 3958
FspBI CTAG 4 cut(s) 791, 2186, 2945, 3707
FspI TGCGCA 1 cut(s) 3958
GlaI GCGC 1 cut(s) 3958
GluI GCNGC 8 cut(s) 2263, 2436, 3126, 3163, 3596, 3599, 3908, 4968
GsaI CCCAGC 1 cut(s) 3866
GsuI CTGGAG 4 cut(s) 1900, 3186, 3388, 3830
HaeIII GGCC 6 cut(s) 9, 1113, 1170, 2721, 3012, 4848
HapII CCGG 5 cut(s) 1538, 3194, 3493, 4447, 4686
HgaI GACGC 1 cut(s) 4933
HhaI GCGC 1 cut(s) 3959
Hin1I GRCGYC 1 cut(s) 3890
Hin6I GCGC 1 cut(s) 3957
HinP1I GCGC 1 cut(s) 3957
HincII GTYRAC 2 cut(s) 1666, 4019
HindII GTYRAC 2 cut(s) 1666, 4019
HindIII AAGCTT 6 cut(s) 741, 1817, 2167, 2732, 2786, 3756
HpaII CCGG 5 cut(s) 1538, 3194, 3493, 4447, 4686
HphI GGTGA 5 cut(s) 1994, 2931, 3265, 4079, 4137
Hpy99I CGWCG 1 cut(s) 1521
HpyCH4III ACNGT 8 cut(s) 891, 2983, 3552, 3766, 4113, 4131, 4263, 4532
HpyF10VI GCNNNNNNNGC 8 cut(s) 1548, 1768, 2417, 2731, 2785, 3965, 4272, 4956
Hsp92I GRCGYC 1 cut(s) 3890
HspAI GCGC 1 cut(s) 3957
Ksp22I TGATCA 2 cut(s) 958, 2848
LguI GCTCTTC 2 cut(s) 1083, 4899
LmnI GCTCC 5 cut(s) 1881, 2176, 2389, 2630, 4215
Lsp1109I GCAGC 5 cut(s) 2249, 3112, 3610, 3894, 4954
MabI ACCWGGT 1 cut(s) 3265
MaeI CTAG 4 cut(s) 791, 2186, 2945, 3707
MaeIII GTNAC 8 cut(s) 772, 1017, 1375, 1752, 2772, 3108, 3402, 3895
MfeI CAATTG 3 cut(s) 216, 941, 3384
MflI RGATCY 4 cut(s) 355, 1534, 1727, 2479
MhlI GDGCHC 4 cut(s) 2902, 4212, 4537, 4789
MlyI GAGTC 8 cut(s) 2124, 2747, 3049, 3797, 4014, 4073, 4387, 4640
MmeI TCCRAC 6 cut(s) 71, 1073, 2154, 3652, 3767, 4866
Mph1103I ATGCAT 4 cut(s) 311, 1482, 1777, 1839
MroXI GAANNNNTTC 5 cut(s) 398, 1077, 1100, 1128, 4505
MslI CAYNNNNRTG 5 cut(s) 314, 1659, 2187, 4752, 4869
MspA1I CMGCKG 3 cut(s) 2873, 3162, 4423
MspCI CTTAAG 3 cut(s) 2605, 2665, 2680
MspI CCGG 5 cut(s) 1538, 3194, 3493, 4447, 4686
MunI CAATTG 3 cut(s) 216, 941, 3384
Mva1269I GAATGC 7 cut(s) 155, 311, 1100, 1529, 1837, 2500, 4718
MvaI CCWGG 5 cut(s) 1115, 2988, 3267, 3809, 4851
MvnI CGCG 1 cut(s) 609
MwoI GCNNNNNNNGC 8 cut(s) 1548, 1768, 2417, 2731, 2785, 3965, 4272, 4956
NciI CCSGG 5 cut(s) 1539, 3194, 4448, 4686, 4687
NdeI CATATG 1 cut(s) 861
NlaIV GGNNCC 9 cut(s) 357, 1729, 1950, 2653, 3013, 3198, 3351, 4211, 4840
NmuCI GTSAC 3 cut(s) 2772, 3108, 3895
NsbI TGCGCA 1 cut(s) 3958
NsiI ATGCAT 4 cut(s) 311, 1482, 1777, 1839
NspI RCATGY 7 cut(s) 276, 497, 1347, 1775, 1837, 1888, 4751
PaeI GCATGC 3 cut(s) 1775, 1888, 4751
PagI TCATGA 1 cut(s) 3049
PciI ACATGT 1 cut(s) 272
PciSI GCTCTTC 2 cut(s) 1083, 4899
PctI GAATGC 7 cut(s) 155, 311, 1100, 1529, 1837, 2500, 4718
PdmI GAANNNNTTC 5 cut(s) 398, 1077, 1100, 1128, 4505
PinAI ACCGGT 1 cut(s) 3492
PkrI GCNGC 8 cut(s) 2264, 2437, 3127, 3164, 3597, 3600, 3909, 4969
PleI GAGTC 8 cut(s) 2124, 2746, 3048, 3796, 4014, 4072, 4386, 4640
PmaCI CACGTG 2 cut(s) 4754, 4784
PmlI CACGTG 2 cut(s) 4754, 4784
PpsI GAGTC 8 cut(s) 2124, 2746, 3048, 3796, 4014, 4072, 4386, 4640
Ppu21I YACGTR 3 cut(s) 1657, 4754, 4784
PscI ACATGT 1 cut(s) 272
PshAI GACNNNNGTC 1 cut(s) 4704
PsiI TTATAA 3 cut(s) 992, 3105, 4302
Psp1406I AACGTT 1 cut(s) 2227
Psp6I CCWGG 5 cut(s) 1113, 2986, 3265, 3807, 4849
PspCI CACGTG 2 cut(s) 4754, 4784
PspEI GGTNACC 1 cut(s) 772
PspFI CCCAGC 1 cut(s) 3862
PspGI CCWGG 5 cut(s) 1113, 2986, 3265, 3807, 4849
PspN4I GGNNCC 9 cut(s) 357, 1729, 1950, 2653, 3013, 3198, 3351, 4211, 4840
PspPI GGNCC 5 cut(s) 2865, 3011, 3505, 4839, 4847
PsrI GAACNNNNNNTAC 4 cut(s) 228, 260, 1002, 1034
PstI CTGCAG 3 cut(s) 706, 2818, 4972
PstNI CAGNNNCTG 3 cut(s) 889, 3692, 3919
PsuI RGATCY 4 cut(s) 355, 1534, 1727, 2479
PvuII CAGCTG 2 cut(s) 2873, 4423
RseI CAYNNNNRTG 5 cut(s) 314, 1659, 2187, 4752, 4869
SalI GTCGAC 1 cut(s) 4017
SapI GCTCTTC 2 cut(s) 1083, 4899
SatI GCNGC 8 cut(s) 2263, 2436, 3126, 3163, 3596, 3599, 3908, 4968
Sau96I GGNCC 5 cut(s) 2865, 3011, 3505, 4839, 4847
SchI GAGTC 8 cut(s) 2124, 2747, 3049, 3797, 4014, 4073, 4387, 4640
SduI GDGCHC 4 cut(s) 2902, 4212, 4537, 4789
SexAI ACCWGGT 1 cut(s) 3265
SfcI CTRYAG 4 cut(s) 702, 2814, 4177, 4968
SinI GGWCC 3 cut(s) 2865, 3505, 4839
SmaI CCCGGG 1 cut(s) 4687
SmiMI CAYNNNNRTG 5 cut(s) 314, 1659, 2187, 4752, 4869
SmlI CTYRAG 9 cut(s) 176, 1133, 1958, 2132, 2384, 2605, 2665, 2680, 2735
SmoI CTYRAG 9 cut(s) 176, 1133, 1958, 2132, 2384, 2605, 2665, 2680, 2735
SphI GCATGC 3 cut(s) 1775, 1888, 4751
SsiI CCGC 5 cut(s) 1610, 2435, 3162, 3595, 4950
SspI AATATT 1 cut(s) 2300
SspMI CTAG 4 cut(s) 791, 2186, 2945, 3707
StyI CCWWGG 6 cut(s) 1207, 1566, 2011, 2467, 3440, 4148
TaaI ACNGT 8 cut(s) 891, 2983, 3552, 3766, 4113, 4131, 4263, 4532
TatI WGTACW 7 cut(s) 496, 847, 1582, 1917, 2825, 4221, 4912
TauI GCSGC 3 cut(s) 2438, 3165, 3598
TscAI CASTG 9 cut(s) 706, 1126, 1534, 2781, 2838, 2902, 3066, 4268, 4831
TseFI GTSAC 3 cut(s) 2772, 3108, 3895
TseI GCWGC 5 cut(s) 2262, 3125, 3598, 3907, 4967
Tsp45I GTSAC 3 cut(s) 2772, 3108, 3895
TspGWI ACGGA 5 cut(s) 599, 2267, 4202, 4700, 4742
TspMI CCCGGG 1 cut(s) 4685
TspRI CASTG 9 cut(s) 706, 1126, 1534, 2781, 2838, 2902, 3066, 4268, 4831
Vha464I CTTAAG 3 cut(s) 2605, 2665, 2680
VneI GTGCAC 1 cut(s) 4533
VpaK11BI GGWCC 3 cut(s) 2865, 3505, 4839
XagI CCTNNNNNAGG 1 cut(s) 4364
XbaI TCTAGA 2 cut(s) 2185, 3706
XceI RCATGY 7 cut(s) 276, 497, 1347, 1775, 1837, 1888, 4751
XmaI CCCGGG 1 cut(s) 4685
XmiI GTMKAC 2 cut(s) 125, 4018
XmnI GAANNNNTTC 5 cut(s) 398, 1077, 1100, 1128, 4505
XspI CTAG 4 cut(s) 791, 2186, 2945, 3707
ZraI GACGTC 1 cut(s) 3891
Zsp2I ATGCAT 4 cut(s) 311, 1482, 1777, 1839
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.