Rroxscaffold_3G00219670

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000129
Physical Location & Seq
Reverse (-)
1768551 .. 1775024
6474 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00219670.1

Sequence Viewer

Length: 4905 bp
ATGACTACCAAACTATATCTTCCTTCTTCTTCTCCTCCTCCATGCCGTAAACTGACATACGATGTGTTCCTCAGTTTCAGAGGTACAGATACCCGCACAAATTTCACAGACCATCTTTATGCCGCTCTCAAGCAGAGAGGAATAAACACATTTAGAGATGATGAAGAACTTAACAGAGGGGAATCAATTGGTCCAAATCTCTTGAAAGGAATTGAAGAGTCAAGATATGCAATTGTGGTGTTTTCTCAGAACTACGCCGATTCTTCGTGGTGTTTGGATGAGCTTGCAAAAGTAGCTGAATGCAGGAGAGCCATGGGACAAACAGTCCTTCCGGTGTTCTACCATGTTGATCCATCTGATGTACGAAGACAAACTGGGGAACACTTTGGGAAGGCATTTGAGAAGCATCAAGAGCGGTTTGGAGGCAATCCTGACAAATTGCAGAGGTGGAAAGACGCTCTTGCTGAAGTAGGCAATCTGTCTGGATGGCATCTGAAAGATGGATACGAATCCAAAGTCATTCAAGAAATTGTTGGAAAGATTTTCACCGAGTTAAATCAATTAATCCCAATATCAGAGGGTTTAGTTGGGATGGATTCTCACCTGCATGAAATGCTTTCGCACTTAGACATTGGGTGTCCGGATGTTCGTATCATAGGGATTTGTGGTATGGGTGGTATCGGTAAGACAACTATTGCACAAGTGGTTTTTGAAAGGGTACAAGCTCAGTTTGAAGGTTGCAGCTTTCTTGAGAATGTGAGAGAGCAGATAACTGAAAAACAAGGTGCAGTTCATTTACAACAGCAACTTCTTTCGGATTTGCTGAAATGTAGTGTAAATGTACAGACCACTAAAACGGGAAAAAAGATAATGAGGCAGAGACTAAGTACTAAAATGGTTCTTATTATTCTTGATGATGTGGACAAAGAAAAACAATTGGAAGCATTGTGTGATAGGGAATCGGTTGGTCCAGGCAGTAGAATCATCATAACTTGTAGAGATGAACATTTGCTCAGTGTAGTTGAAGGAGACAGAGTATATAAGGTGAATCCATTAACTGATGCTGAAGCTCTTCAGCTTTTCAGTATGAAAGCCTTCAAGAAAGACCAGCTGGTTGGAAAAGAAATTTTGAAGCTATCCAAAGAATTGTTGAAATATGCTAATGGCCTTCCACTAGCTATTGAAGTTTTGGGTTGTCATGTTAAGGGTAGAGGGGTAGAGTTATGGTCAAGTGCATTGGAAAGACTTAAAGAAAGTCCTGATAAAGAAATTATTAATGTTCTCAAGGTTAGTTTTGATGGATTAGAACCAAGGGAACAGAAAATTTTTTTGGACATCGCATGTTTCCTTAAGGGGGTGAACAGAGATCGAGTAAGAAGAATACTACAGGGTAGTAATGCCCACTGTCCAGACTTTGATATACAGGTTCTCCTGGATAAATCTATGGTAACTCTATTTGGAGAAAAATTGTGGATGCATGATTTGATACAAGAAATGGGTCGAGAAGTTGTTCTTCAAGAATGCCGTGAAGAGCCTGGAAAACGTAGCAGATTGTGGCTTCCAAATGAGATTATCCATGTACTTGATCGGAGTAAGGCGACGAGTGCAGTTCAAAGCATATTCCTCCAATGTCCAACAAAAGACAATATTGTTCACTCAATTGATATTGCCTTCTCAAACATGGACAGATTGAGGTTGCTCAAGATTTGGAATTTGAAGTGTACTGGAAACATCAGATATCTTTCTAATGAGTTACAGTATCTTGAATGGCATGAGTGTCCTTTGGTTTCTTTCCCGTCAGACTTTCAACCGGATAAACTAGTGGAAGTTCACATGTGTTTCAGCCGCATCGAACAACTATGGAGGGGAAAAAAGGGATGGAGCATGCTACGGCTTATTGATCTGACTGGTTCACAATACTTGATGTCGACTCCAGACTTCACTGAGGTTCCTGATCTTGAAACGTTGGTGCTTCAATGTTGTACAAGCTTGGTGGAGGTTCACCCGTCTCTTGGATTTCTGAAGAAACTTGTCTCTTTGAATATGACATGTTGCATGTCTATTGAGAGCCTTCCACCTTTCACTGCCTTGGAATCCCTTCAAATATTGAGGCTTTCATTCTGTTCAGCACTGAAGAAGTTTCCAGAAATTGACGGAAATATGAAAAGCTTGCTGGAGCTTTATTTGGATGGGACCAGCATTGAGGAATTGCCTCCATCAATTGAACGCTTGACTGGTCTTACCATGTTGAGTCTAGCACACTGTAAAAACCTTTTGCATCTTCCCAATGCAATTGGGTGCCTGACATCTTTGAAAAGTCTCCATCTAACTGGTTGCTCCAATATTGATGAGATACCTGAGAATCTGAATGGTCTGAAATGTCTGGAGACGCTTGCAATTGGTGGAACTTCCATAAGGGAGTTATCTTTCATTGTAGGCATGAAGAATCTACACTATCTATCCTGCGGAGGATGTAAATTTCTAGTTTCAGAATCATGCAATGTCCTGGCTTCGTTATCGAATTTGATAGAGCTAGACTTGAGTTATTGCAATCTGATGGATGGAGCATTTCTCAATGATTTTAGCAGCCTAATCTCCTTGGAAGTGTTAGATTTACGTGGTAATTGCTTTGTGCGATTACCTGAAAGTATCTCCCAACTCTCAAAGCTCGGCACTCTGGACTTGAGGAATTGCAGACAGCTTCAATTGCTGCCCAAGAAGCTTCCGTTAAGTCTTAGTTACGTGAGTGCGCAAGATTGTACTTCACTGACGGATTACCCAAACCAAATCGAAGTATTGAATTCATCGGAGTCGGGAGTGACTGTTGTCAATTCCCTCAATTCTTCAGCACTTGGAGACCCACAAAGTTCCGAAATTGGCCGACATCCGCTCCTCCTGACAACCCCGTACAAGTCACCCCGAAATTGGCCGCCTAATTTAAGAGATGAGGAGGACTATATGTACCTGTACGCTCGTCCAGAAAATGTGGGGGAATCTTCCCAACTGCGTGTGACACATAAGCATACAGAGGGGGAACACGTTTATCATTTGCCACCGACAGCTCAATCCCATATAAATCAAATGTCTTCCTGGGCAAAATTCGCTGCCGTACATCCTAGCGATGAAATTCCAGAGTGGTTCAGCACTACATCTATCAGAAATCCCATGTCAATCTCGATACCTCAAAATCTGGCCGATGATGACAAGTGGAAGGGATTTGCCGCGTGTGCTGTATTCTCAGTCAAGGGGCATACTGCTCTGACCCTTATTGAACCGGATCCTGATTTTCTTAATTACAACTATCAAATCACATTGGAAACTGATGTTGTGCGTCTGAAACAATTAGTTGTGTCTGGAGTGGATATATTGGAGGGCTGCTTTGGATTAAGTTCTCATCTGCTTGTGATTTTCGGTATATCTAGTTTGGAGGTTCCAGAGAGGGGGTTAAATCAATCAACTGTGATGAGGGCTATATTTGAAACCACCAATCCATCCATGGTGGTCCAGGAATGTGGAATTCGTCTGGTCTATGAGCAAGATGCTGGATGGCATTCCAGAATACTCGGGGGCGTTGATTCGATTTGTCTGAATGAAGTTGAGGACCACGCCTCTGTACGGGAGTGTGCCTGGCTTAGTGAATCTGATAAATGGGAGAAATTCATTCCACCCTTGGAAGAAGAATCCACACTTGTGCTGAGAAAGAACCTTGAGTCTGTTCTTCGAAGATACCTTGAGGCACTAAATTGTTATCCTAAAACGTACAAATTTAACCTCAGTGGAAGGCCAGGGTGGTTTCATGAGTCGTTTGCCTTTCGTGAGAGGCCAGATTTTACCGTAGTGTCAATCAAGCGGCCTCAAAATTTACACAAGAGCAAGAAGTGGATGGGATTTGCAATATATGCATCACTTGTAGAGGAAGTTGGGCAGACGATGACAGAGGAAAACTATCAGATATCTTTTATACTAACAACTGATAAATCTCATATGAGTATGACCGGAGAATTTAATCACGTCAGGCCATTGTCAGAAGAACATCATCGACTATTGGTTATTTACATACCGCGGGAACAGATTCCCGAAGTGTTGTTCACTCAGACATTAACAACTGCAATGGACTTTTGCATCAAGATCAGTGATGGCCCTCATGTAAAGGTTCAAAGATGTGGGTTCCGTATTGTGTACCAAGAAGATATACAAGGATTTGCTGATACAATTATCCGGTGCATGCAAAGGGAAGATTCTCTTAAATCCTACAATAAGTTGGTGGTAGAAGAATGGATACTATTGATACATTTGCAAGGTGCCCTAAATTTAGAGAGAATGACAGAACGGGATTCCAGAACCCCACGGGAAAAACAATTTGAATTGCACTTACAAAAATATAGAAATTGGGACTGGTCTGACCCTCGTTCTTTTTTTTGTCACTTCGAGCGAGTTCAAATCTCCAAGTTGAAATGGTTCATGCCTTTCATCAACCAAAGCAACTCTGTACAAATCCAACTGCCTCTGAATGTGTTTGATGATTATAATTGGTTGGGGTTTGCCGTATGTTCTCAATTGTCCCACGACCAAAATCCAAACATTAGTCTTGGCAGTACTGCTCCTGATTCAGAGGATGTTACGCTTAATTGCTGTCTGGCCTCTGATGTTGCTTGGGAGGGCTTATTTGATTACACGACTGAAGTAAACATTCGAGAGCCATCGGAATACGATGCGACTTGGTTCTTTTATATACCGCGCACTAAAGTTCGTAGTAAAGTCTGGAGGCAATGCAAATTGGCCAGGATAACTATTTGGTTGTCTAGCCCAGACTCAGCAGTAGTGCATAATTGTGCCCTTCGTCTACTATTTAAGGAGGACGTTGAACATCTTGTAGAAACACTGACCCTCGCCGAATTACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1634

Amino Acids

185.87

Weight (kDa)

5.74

Isoelectric Point (pI)

45.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 20 - 191 1.1e-54 TIR domain
TIR_2 PF13676 22 - 118 8.7e-16 TIR domain
NB-ARC PF00931 201 - 369 1.2e-22 NB-ARC domain
WHD_ROQ1 PF23282 438 - 505 3.1e-15 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 668 - 778 4.1e-07 Leucine-rich repeat region
LRR_13 PF23286 697 - 784 1.4e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 836 - 908 6.5e-07 Leucine-rich repeat region
C-JID PF20160 1039 - 1181 2.6e-09 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4527
AarI CACCTGC 1 cut(s) 612
Acc16I TGCGCA 1 cut(s) 2751
Acc36I ACCTGC 1 cut(s) 612
AccB1I GGYRCC 2 cut(s) 2298, 4301
AccBSI CCGCTC 3 cut(s) 125, 415, 2890
AccI GTMKAC 2 cut(s) 1928, 4843
AccII CGCG 3 cut(s) 3224, 4063, 4738
AccIII TCCGGA 1 cut(s) 640
AclI AACGTT 1 cut(s) 1964
AclWI GGATC 3 cut(s) 344, 3272, 3285
AcoI YGGCCR 4 cut(s) 2878, 2927, 3192, 4779
AcuI CTGAAG 7 cut(s) 486, 1058, 1086, 2042, 2153, 2829, 4701
AfiI CCNNNNNNNGG 5 cut(s) 1354, 2012, 2925, 3440, 3615
AflII CTTAAG 1 cut(s) 1349
AflIII ACRYGT 3 cut(s) 1833, 2048, 3037
AhdI GACNNNNNGTC 1 cut(s) 323
AhlI ACTAGT 1 cut(s) 1819
AjiI CACGTC 1 cut(s) 4012
AjnI CCWGG 9 cut(s) 970, 1431, 1534, 2505, 3089, 3504, 3626, 3784, 4781
AjuI GAANNNNNNNTTGG 2 cut(s) 1313, 1345
AleI CACNNNNGTG 1 cut(s) 3689
Alw26I GTCTC 7 cut(s) 874, 1023, 2013, 2038, 2324, 2381, 2850
AlwI GGATC 3 cut(s) 344, 3272, 3285
Ama87I CYCGRG 1 cut(s) 3563
Aor13HI TCCGGA 1 cut(s) 640
ApeKI GCWGC 5 cut(s) 741, 2586, 2710, 3104, 3375
ArsI GACNNNNNNTTYG 2 cut(s) 501, 533
AseI ATTAAT 2 cut(s) 563, 1275
Asp700I GAANNNNTTC 6 cut(s) 1071, 1094, 1509, 2097, 4071, 4457
AspLEI GCGC 2 cut(s) 2752, 4740
AspS9I GGNCC 6 cut(s) 191, 968, 2193, 3502, 3601, 4139
AsuHPI GGTGA 6 cut(s) 538, 593, 1057, 1369, 1994, 2907
AsuII TTCGAA 1 cut(s) 3721
AvaI CYCGRG 1 cut(s) 3563
AvaII GGWCC 5 cut(s) 191, 968, 2193, 3502, 3601
BaeGI GKGCMC 2 cut(s) 4306, 4837
BalI TGGCCA 1 cut(s) 4781
BamHI GGATCC 1 cut(s) 3277
BanI GGYRCC 2 cut(s) 2298, 4301
BarI GAAGNNNNNNTAC 2 cut(s) 4263, 4295
BbsI GAAGAC 2 cut(s) 373, 3078
BbvI GCAGC 5 cut(s) 753, 2598, 2697, 3091, 3362
BceAI ACGGC 5 cut(s) 30, 1509, 1907, 3092, 4529
BciT130I CCWGG 9 cut(s) 972, 1433, 1536, 2507, 3091, 3506, 3628, 3786, 4783
BciVI GTATCC 2 cut(s) 497, 4272
BcoDI GTCTC 7 cut(s) 874, 1023, 2013, 2038, 2324, 2381, 2850
BcuI ACTAGT 1 cut(s) 1819
BfaI CTAG 8 cut(s) 1175, 1820, 2255, 2483, 2534, 3117, 3420, 4803
BfmI CTRYAG 1 cut(s) 1385
BfrI CTTAAG 1 cut(s) 1349
BfuAI ACCTGC 1 cut(s) 612
BfuI GTATCC 2 cut(s) 497, 4272
BmcAI AGTACT 2 cut(s) 889, 4597
Bme1390I CCNGG 9 cut(s) 972, 1433, 1536, 2507, 3091, 3506, 3628, 3786, 4783
Bme18I GGWCC 5 cut(s) 191, 968, 2193, 3502, 3601
BmeRI GACNNNNNGTC 1 cut(s) 323
BmeT110I CYCGRG 1 cut(s) 3563
BmgBI CACGTC 1 cut(s) 4012
BmgT120I GGNCC 6 cut(s) 191, 968, 2193, 3502, 3601, 4139
BmiI GGNNCC 7 cut(s) 1950, 2194, 2300, 3279, 3432, 4169, 4303
BmrFI CCNGG 9 cut(s) 972, 1433, 1536, 2507, 3091, 3506, 3628, 3786, 4783
BmrI ACTGGG 1 cut(s) 384
BmuI ACTGGG 1 cut(s) 384
BoxI GACNNNNGTC 1 cut(s) 2825
BpiI GAAGAC 2 cut(s) 373, 3078
BplI GAGNNNNNCTC 2 cut(s) 2556, 2588
BpmI CTGGAG 5 cut(s) 1917, 2195, 2405, 3375, 4783
Bpu14I TTCGAA 1 cut(s) 3721
BpuEI CTTGAG 8 cut(s) 113, 770, 1268, 1685, 2560, 2704, 3728, 3752
BsaAI YACGTR 2 cut(s) 2618, 2743
BsaBI GATNNNNATC 1 cut(s) 508
BsaI GGTCTC 1 cut(s) 2850
BsaWI WCCGGW 6 cut(s) 331, 640, 1810, 3274, 3995, 4218
BsaXI ACNNNNNCTCC 8 cut(s) 1020, 1050, 1413, 1443, 2808, 2838, 2874, 2904
Bsc4I CCNNNNNNNGG 5 cut(s) 1354, 2012, 2925, 3440, 3615
Bse1I ACTGG 6 cut(s) 379, 1729, 1912, 2239, 2335, 4400
Bse3DI GCAATG 3 cut(s) 2506, 4116, 4775
Bse8I GATNNNNATC 1 cut(s) 508
BseAI TCCGGA 1 cut(s) 640
BseBI CCWGG 9 cut(s) 972, 1433, 1536, 2507, 3091, 3506, 3628, 3786, 4783
BseJI GATNNNNATC 1 cut(s) 508
BseLI CCNNNNNNNGG 5 cut(s) 1354, 2012, 2925, 3440, 3615
BseMI GCAATG 3 cut(s) 2506, 4116, 4775
BseNI ACTGG 6 cut(s) 379, 1729, 1912, 2239, 2335, 4400
BseRI GAGGAG 4 cut(s) 24, 27, 2882, 2963
BseSI GKGCMC 2 cut(s) 4306, 4837
BseXI GCAGC 5 cut(s) 753, 2598, 2697, 3091, 3362
BsgI GTGCAG 2 cut(s) 807, 1626
Bsh1236I CGCG 3 cut(s) 3224, 4063, 4738
BshNI GGYRCC 2 cut(s) 2298, 4301
BsiHKCI CYCGRG 1 cut(s) 3563
BsiSI CCGG 6 cut(s) 332, 641, 1811, 3275, 3996, 4219
BslFI GGGAC 4 cut(s) 330, 2206, 4406, 4546
BslI CCNNNNNNNGG 5 cut(s) 1354, 2012, 2925, 3440, 3615
BsmAI GTCTC 7 cut(s) 874, 1023, 2013, 2038, 2324, 2381, 2850
BsmBI CGTCTC 2 cut(s) 2013, 2381
BsmFI GGGAC 4 cut(s) 330, 2206, 4406, 4546
BsmI GAATGC 3 cut(s) 305, 1526, 3550
Bso31I GGTCTC 1 cut(s) 2850
BsoBI CYCGRG 1 cut(s) 3563
Bsp119I TTCGAA 1 cut(s) 3721
Bsp1286I GDGCHC 2 cut(s) 4306, 4837
Bsp13I TCCGGA 1 cut(s) 640
Bsp1407I TGTACA 3 cut(s) 841, 1982, 4489
Bsp143I GATC 7 cut(s) 349, 1366, 1585, 1900, 1954, 3277, 4128
Bsp19I CCATGG 2 cut(s) 312, 3495
BspEI TCCGGA 1 cut(s) 640
BspFNI CGCG 3 cut(s) 3224, 4063, 4738
BspHI TCATGA 1 cut(s) 3796
BspLI GGNNCC 7 cut(s) 1950, 2194, 2300, 3279, 3432, 4169, 4303
BspMI ACCTGC 1 cut(s) 612
BspPI GGATC 3 cut(s) 344, 3272, 3285
BspQI GCTCTTC 2 cut(s) 1077, 1524
BspT104I TTCGAA 1 cut(s) 3721
BspT107I GGYRCC 2 cut(s) 2298, 4301
BspTI CTTAAG 1 cut(s) 1349
BspTNI GGTCTC 1 cut(s) 2850
BsrBI CCGCTC 3 cut(s) 125, 415, 2890
BsrDI GCAATG 3 cut(s) 2506, 4116, 4775
BsrGI TGTACA 3 cut(s) 841, 1982, 4489
BsrI ACTGG 6 cut(s) 379, 1729, 1912, 2239, 2335, 4400
BssMI GATC 7 cut(s) 349, 1366, 1585, 1900, 1954, 3277, 4128
BssT1I CCWWGG 6 cut(s) 312, 1310, 2088, 2598, 3495, 3669
Bst2UI CCWGG 9 cut(s) 972, 1433, 1536, 2507, 3091, 3506, 3628, 3786, 4783
Bst4CI ACNGT 7 cut(s) 325, 1406, 1758, 2264, 2824, 3460, 3835
Bst6I CTCTTC 3 cut(s) 210, 1077, 1524
BstAFI CTTAAG 1 cut(s) 1349
BstAPI GCANNNNNTGC 2 cut(s) 613, 3899
BstAUI TGTACA 3 cut(s) 841, 1982, 4489
BstBAI YACGTR 2 cut(s) 2618, 2743
BstBI TTCGAA 1 cut(s) 3721
BstC8I GCNNGC 5 cut(s) 285, 1886, 2171, 2394, 4226
BstDSI CCRYGG 4 cut(s) 312, 3495, 4061, 4346
BstFNI CGCG 3 cut(s) 3224, 4063, 4738
BstHHI GCGC 2 cut(s) 2752, 4740
BstKTI GATC 7 cut(s) 352, 1369, 1588, 1903, 1957, 3280, 4131
BstMAI GTCTC 7 cut(s) 874, 1023, 2013, 2038, 2324, 2381, 2850
BstMBI GATC 7 cut(s) 349, 1366, 1585, 1900, 1954, 3277, 4128
BstMWI GCNNNNNNNGC 9 cut(s) 293, 412, 613, 1604, 2707, 2719, 3101, 3227, 3899
BstNI CCWGG 9 cut(s) 972, 1433, 1536, 2507, 3091, 3506, 3628, 3786, 4783
BstNSI RCATGY 6 cut(s) 1344, 1837, 1888, 2052, 2059, 4228
BstPAI GACNNNNGTC 1 cut(s) 2825
BstSCI CCNGG 9 cut(s) 970, 1431, 1534, 2505, 3089, 3504, 3626, 3784, 4781
BstSFI CTRYAG 1 cut(s) 1385
BstSLI GKGCMC 2 cut(s) 4306, 4837
BstUI CGCG 3 cut(s) 3224, 4063, 4738
BstV1I GCAGC 5 cut(s) 753, 2598, 2697, 3091, 3362
BstV2I GAAGAC 2 cut(s) 373, 3078
BstX2I RGATCY 1 cut(s) 3277
BstXI CCANNNNNNTGG 3 cut(s) 1115, 2330, 3512
BstYI RGATCY 1 cut(s) 3277
BsuI GTATCC 2 cut(s) 497, 4272
BtgI CCRYGG 4 cut(s) 312, 3495, 4061, 4346
BtgZI GCGATG 2 cut(s) 1321, 3135
BtrI CACGTC 1 cut(s) 4012
BtsI GCAGTG 1 cut(s) 2082
BveI ACCTGC 1 cut(s) 612
Cac8I GCNNGC 5 cut(s) 285, 1886, 2171, 2394, 4226
CciI TCATGA 1 cut(s) 3796
CfoI GCGC 2 cut(s) 2752, 4740
Cfr13I GGNCC 6 cut(s) 191, 968, 2193, 3502, 3601, 4139
Cfr42I CCGCGG 1 cut(s) 4064
CseI GACGC 3 cut(s) 464, 2398, 3320
CspCI CAANNNNNGTGG 2 cut(s) 1974, 2009
DpnI GATC 7 cut(s) 351, 1368, 1587, 1902, 1956, 3279, 4130
DpnII GATC 7 cut(s) 349, 1366, 1585, 1900, 1954, 3277, 4128
DriI GACNNNNNGTC 1 cut(s) 323
EaeI YGGCCR 4 cut(s) 2878, 2927, 3192, 4779
Eam1104I CTCTTC 3 cut(s) 210, 1077, 1524
Eam1105I GACNNNNNGTC 1 cut(s) 323
EarI CTCTTC 3 cut(s) 210, 1077, 1524
Eco130I CCWWGG 6 cut(s) 312, 1310, 2088, 2598, 3495, 3669
Eco31I GGTCTC 1 cut(s) 2850
Eco32I GATATC 2 cut(s) 1739, 3954
Eco47I GGWCC 5 cut(s) 191, 968, 2193, 3502, 3601
Eco57I CTGAAG 7 cut(s) 486, 1058, 1086, 2042, 2153, 2829, 4701
Eco88I CYCGRG 1 cut(s) 3563
EcoRI GAATTC 2 cut(s) 2800, 3516
EcoRII CCWGG 9 cut(s) 970, 1431, 1534, 2505, 3089, 3504, 3626, 3784, 4781
EcoRV GATATC 2 cut(s) 1739, 3954
EcoT14I CCWWGG 6 cut(s) 312, 1310, 2088, 2598, 3495, 3669
EcoT22I ATGCAT 2 cut(s) 1479, 3904
ErhI CCWWGG 6 cut(s) 312, 1310, 2088, 2598, 3495, 3669
Esp3I CGTCTC 2 cut(s) 2013, 2381
FalI AAGNNNNNCTT 6 cut(s) 444, 476, 1497, 1529, 4227, 4259
FaqI GGGAC 4 cut(s) 330, 2206, 4406, 4546
FauI CCCGC 2 cut(s) 101, 4056
FauNDI CATATG 1 cut(s) 3984
FblI GTMKAC 2 cut(s) 1928, 4843
FspBI CTAG 8 cut(s) 1175, 1820, 2255, 2483, 2534, 3117, 3420, 4803
FspI TGCGCA 1 cut(s) 2751
GlaI GCGC 2 cut(s) 2751, 4739
GsuI CTGGAG 5 cut(s) 1917, 2195, 2405, 3375, 4783
HapII CCGG 6 cut(s) 332, 641, 1811, 3275, 3996, 4219
HgaI GACGC 3 cut(s) 464, 2398, 3320
HhaI GCGC 2 cut(s) 2752, 4740
Hin6I GCGC 2 cut(s) 2750, 4738
HinP1I GCGC 2 cut(s) 2750, 4738
HincII GTYRAC 1 cut(s) 1929
HindII GTYRAC 1 cut(s) 1929
HindIII AAGCTT 3 cut(s) 1987, 2167, 2720
HpaII CCGG 6 cut(s) 332, 641, 1811, 3275, 3996, 4219
HphI GGTGA 6 cut(s) 538, 593, 1057, 1369, 1994, 2907
Hpy99I CGWCG 1 cut(s) 1603
HpyCH4III ACNGT 7 cut(s) 325, 1406, 1758, 2264, 2824, 3460, 3835
HpyCH4IV ACGT 8 cut(s) 1543, 1964, 2617, 2742, 3039, 3758, 4011, 4860
HpyF10VI GCNNNNNNNGC 9 cut(s) 293, 412, 613, 1604, 2707, 2719, 3101, 3227, 3899
HpySE526I ACGT 8 cut(s) 1543, 1964, 2617, 2742, 3039, 3758, 4011, 4860
HspAI GCGC 2 cut(s) 2750, 4738
Kpn2I TCCGGA 1 cut(s) 640
KspI CCGCGG 1 cut(s) 4064
Kzo9I GATC 7 cut(s) 349, 1366, 1585, 1900, 1954, 3277, 4128
LguI GCTCTTC 2 cut(s) 1077, 1524
LmnI GCTCC 6 cut(s) 1881, 2176, 2342, 2564, 2895, 4606
Lsp1109I GCAGC 5 cut(s) 753, 2598, 2697, 3091, 3362
MaeI CTAG 8 cut(s) 1175, 1820, 2255, 2483, 2534, 3117, 3420, 4803
MaeII ACGT 8 cut(s) 1543, 1964, 2617, 2742, 3039, 3758, 4011, 4860
MaeIII GTNAC 8 cut(s) 1447, 1752, 2738, 2818, 2913, 3010, 4421, 4618
MalI GATC 7 cut(s) 351, 1368, 1587, 1902, 1956, 3279, 4130
MbiI CCGCTC 3 cut(s) 125, 415, 2890
MboI GATC 7 cut(s) 349, 1366, 1585, 1900, 1954, 3277, 4128
MfeI CAATTG 9 cut(s) 186, 231, 935, 1659, 2220, 2292, 2397, 2705, 4556
MflI RGATCY 1 cut(s) 3277
MhlI GDGCHC 2 cut(s) 4306, 4837
MlsI TGGCCA 1 cut(s) 4781
MluNI TGGCCA 1 cut(s) 4781
MlyI GAGTC 7 cut(s) 227, 1924, 2260, 2819, 3719, 3809, 4805
MmeI TCCRAC 4 cut(s) 514, 1096, 1658, 4522
Mox20I TGGCCA 1 cut(s) 4781
Mph1103I ATGCAT 2 cut(s) 1479, 3904
MroI TCCGGA 1 cut(s) 640
MroXI GAANNNNTTC 6 cut(s) 1071, 1094, 1509, 2097, 4071, 4457
MscI TGGCCA 1 cut(s) 4781
MslI CAYNNNNRTG 4 cut(s) 117, 606, 3689, 4830
Msp20I TGGCCA 1 cut(s) 4781
MspA1I CMGCKG 2 cut(s) 1111, 4063
MspCI CTTAAG 1 cut(s) 1349
MspI CCGG 6 cut(s) 332, 641, 1811, 3275, 3996, 4219
MspR9I CCNGG 9 cut(s) 972, 1433, 1536, 2507, 3091, 3506, 3628, 3786, 4783
MunI CAATTG 9 cut(s) 186, 231, 935, 1659, 2220, 2292, 2397, 2705, 4556
Mva1269I GAATGC 3 cut(s) 305, 1526, 3550
MvaI CCWGG 9 cut(s) 972, 1433, 1536, 2507, 3091, 3506, 3628, 3786, 4783
MvnI CGCG 3 cut(s) 3224, 4063, 4738
MwoI GCNNNNNNNGC 9 cut(s) 293, 412, 613, 1604, 2707, 2719, 3101, 3227, 3899
NcoI CCATGG 2 cut(s) 312, 3495
NdeI CATATG 1 cut(s) 3984
NdeII GATC 7 cut(s) 349, 1366, 1585, 1900, 1954, 3277, 4128
NlaIV GGNNCC 7 cut(s) 1950, 2194, 2300, 3279, 3432, 4169, 4303
NmeAIII GCCGAG 1 cut(s) 2649
NmuCI GTSAC 4 cut(s) 2818, 2913, 3010, 4421
NsbI TGCGCA 1 cut(s) 2751
NsiI ATGCAT 2 cut(s) 1479, 3904
NspI RCATGY 6 cut(s) 1344, 1837, 1888, 2052, 2059, 4228
NspV TTCGAA 1 cut(s) 3721
OliI CACNNNNGTG 1 cut(s) 3689
PaeI GCATGC 2 cut(s) 1888, 4228
PagI TCATGA 1 cut(s) 3796
PaqCI CACCTGC 1 cut(s) 612
PciI ACATGT 2 cut(s) 1833, 2048
PciSI GCTCTTC 2 cut(s) 1077, 1524
PctI GAATGC 3 cut(s) 305, 1526, 3550
PdmI GAANNNNTTC 6 cut(s) 1071, 1094, 1509, 2097, 4071, 4457
PfoI TCCNGGA 2 cut(s) 1431, 3504
PleI GAGTC 7 cut(s) 226, 1924, 2259, 2818, 3718, 3808, 4805
PpsI GAGTC 7 cut(s) 226, 1924, 2259, 2818, 3718, 3808, 4805
Ppu21I YACGTR 2 cut(s) 2618, 2743
PscI ACATGT 2 cut(s) 1833, 2048
PshAI GACNNNNGTC 1 cut(s) 2825
PshBI ATTAAT 2 cut(s) 563, 1275
PsiI TTATAA 1 cut(s) 4527
Psp1406I AACGTT 1 cut(s) 1964
Psp6I CCWGG 9 cut(s) 970, 1431, 1534, 2505, 3089, 3504, 3626, 3784, 4781
PspGI CCWGG 9 cut(s) 970, 1431, 1534, 2505, 3089, 3504, 3626, 3784, 4781
PspN4I GGNNCC 7 cut(s) 1950, 2194, 2300, 3279, 3432, 4169, 4303
PspPI GGNCC 6 cut(s) 191, 968, 2193, 3502, 3601, 4139
PsuI RGATCY 1 cut(s) 3277
PvuII CAGCTG 1 cut(s) 1111
RseI CAYNNNNRTG 4 cut(s) 117, 606, 3689, 4830
SacII CCGCGG 1 cut(s) 4064
SalI GTCGAC 1 cut(s) 1927
SapI GCTCTTC 2 cut(s) 1077, 1524
Sau3AI GATC 7 cut(s) 349, 1366, 1585, 1900, 1954, 3277, 4128
Sau96I GGNCC 6 cut(s) 191, 968, 2193, 3502, 3601, 4139
ScaI AGTACT 2 cut(s) 889, 4597
SchI GAGTC 7 cut(s) 227, 1924, 2260, 2819, 3719, 3809, 4805
ScrFI CCNGG 9 cut(s) 972, 1433, 1536, 2507, 3091, 3506, 3628, 3786, 4783
SduI GDGCHC 2 cut(s) 4306, 4837
SfcI CTRYAG 1 cut(s) 1385
Sfr303I CCGCGG 1 cut(s) 4064
SfuI TTCGAA 1 cut(s) 3721
SgrBI CCGCGG 1 cut(s) 4064
SinI GGWCC 5 cut(s) 191, 968, 2193, 3502, 3601
SmiMI CAYNNNNRTG 4 cut(s) 117, 606, 3689, 4830
SmlI CTYRAG 9 cut(s) 128, 749, 1283, 1349, 1700, 2539, 2683, 3707, 3731
SmoI CTYRAG 9 cut(s) 128, 749, 1283, 1349, 1700, 2539, 2683, 3707, 3731
SpeI ACTAGT 1 cut(s) 1819
SphI GCATGC 2 cut(s) 1888, 4228
SspI AATATT 3 cut(s) 1648, 2106, 2344
SspMI CTAG 8 cut(s) 1175, 1820, 2255, 2483, 2534, 3117, 3420, 4803
StyD4I CCNGG 9 cut(s) 970, 1431, 1534, 2505, 3089, 3504, 3626, 3784, 4781
StyI CCWWGG 6 cut(s) 312, 1310, 2088, 2598, 3495, 3669
TaaI ACNGT 7 cut(s) 325, 1406, 1758, 2264, 2824, 3460, 3835
TaiI ACGT 8 cut(s) 1546, 1967, 2620, 2745, 3042, 3761, 4014, 4863
TatI WGTACW 8 cut(s) 841, 887, 1579, 1721, 1982, 2759, 4489, 4595
TauI GCSGC 5 cut(s) 125, 1848, 2932, 3224, 3853
TseFI GTSAC 4 cut(s) 2818, 2913, 3010, 4421
TseI GCWGC 5 cut(s) 741, 2586, 2710, 3104, 3375
Tsp45I GTSAC 4 cut(s) 2818, 2913, 3010, 4421
TspGWI ACGGA 4 cut(s) 2169, 2715, 2786, 4160
Vha464I CTTAAG 1 cut(s) 1349
VpaK11BI GGWCC 5 cut(s) 191, 968, 2193, 3502, 3601
VspI ATTAAT 2 cut(s) 563, 1275
XceI RCATGY 6 cut(s) 1344, 1837, 1888, 2052, 2059, 4228
XcmI CCANNNNNNNNNTGG 1 cut(s) 3493
XmiI GTMKAC 2 cut(s) 1928, 4843
XmnI GAANNNNTTC 6 cut(s) 1071, 1094, 1509, 2097, 4071, 4457
XspI CTAG 8 cut(s) 1175, 1820, 2255, 2483, 2534, 3117, 3420, 4803
ZrmI AGTACT 2 cut(s) 889, 4597
Zsp2I ATGCAT 2 cut(s) 1479, 3904
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.