Rw7G041250

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000129
Physical Location & Seq
Forward (+)
64259215 .. 64265428
6214 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G041250.1

Sequence Viewer

Length: 5037 bp
ATGGGTTTCCCTGTCCGGCTTATCCTCTTGTCTTCCATTTGCCCGCTACTTTTTTTCTCTGCTCTGTTTTTCTGTGGCTTTATCTCGTTAGGGTTGTACCTGTTGTTCTTCTTCTCCACTGTTTCTATTTGTTGTTGCCTTTTGGCTTTCAATCTTCGCCATAAACAGAGACCAGTAACACTACTGATCCACACTTCTTCAGTTACCACCAATACAGATATAATTCCTTCTTCTTCTTCTTCTTCTTCTTCGTCGTCGTTTTCTTCTCCACCATCCGGTAAACTCACGTACGATGTGTTCCTTAGTTTCAGAGGTACAGATACCCGCAAAAATTTCACAGACCATCTTTATACTGCTCTCAAACAGAAAGGTATATTCACTTTTAGAGACGATGAAGAACTCGAGAGGGGGGAATCGATTGGTCCAAATCTCTTGAAAGCAATCGAAGAATCAAGATATGTAATTGCTGTTTTCTCACGAAACTACGCCGATTCTGCATGGTGTTTGGATGAGATTGCCAAGATCGCCGATTGCATGAAAGCCATGGGACAAAAAGTCCTCCCAGTGTTCTACCATGTTGATCCATCTGAGGTACGAAGACAAACAGGGGATCACTTTGGGAAAGCATTTGAGAAGCATCAGAGGCGGTATAAAGCCGAACCAGACAAAGTAAAGAGGTGGAAGGACGCTCTTTTTCAAGTAGGCAATCTCTCTGGATGGCATCTGCAAGATGGGTACGAATCCAAAGTTATTCAAGAAATTGTTGAAAAGATTTTCACCGAGTTAAATCAAATAATCTCAATATCCGAGGGTTTAGTTGGCATGGATTCTCACCTGAATGAAATGCTTTCATACTTGGACATTGGGTGTCCGGATGTTCGTATCATAGGGATTTGTGGGATGGGTGGTATCGGTAAGACAACTATTGCACAAGTGGTTTTTGAAAGGGTACAAGCTCAGTTTGATGGTTGCAGCTTTCTTGAGAATGTTAGAGAGGTAATTGAAAAGCAAGGTGCAGTGCATTTACAAGAGCAACTTCTTTCGAATTTGCTGAGATTTAGTGTAAATGCAGAGGTATGTACTAAAATGGTTCTTCTCATTCTTGATGATGTGGACCAAGAAGAACAATTGGAAGCATTGTGTGATAGGGAATCGTTTGGTCCAGGGAGTAGGATCATCATAACTTCTAGAGATGAACATTTGCTCAGTGCAGTTGAAGGAGATAAAGTATATAAGGTGAATCCATTAACTGATGCTGAAGCTCTTCAGCTTTTCAGTATGAAAGCCTTTAAGAAAGACCAGCTGGTTGGAGAAGAAATTCTGAAGCTATCCAAAGAGTTTTTGAAATATGCTAATGGCCTTCCACTAGCTATTAAAGTTTTGGGTTCTTCTGTTAAGGGTAGAAGTGTAGACTTATGGTTAAGTGCATTGGATAGACTTAAAGAAAATCCTGAAAAAAAAATTATTAATGTTCTCAAAGTTAGTTTTGATGGATTAGAACCAAGAGAAAAGAAGATTTTTTTGGACATTGCATGTTTCTTTAAAGGGGAGAGCATAGATCGTGTAAGAAGAATATTACAAGGTAGTGATGCCCACTGTCCGGACTTTGATGTACAAGTTCTCCTGGACAAATCTATGATAACTGTATTTGGAAAAAGATTGTGGATGCATGATTTGATACAACAGTTAGGTTGGGAAGTTGTCCGTCAAGAATGTCCTGAAGAGCCTGGAAAACGTAGTCGATTGTGGCTTCCAAATGAGATCATTGATGTTCTTGATCAGAATAAGGCAACCACTGCAGTTCAAAGCATACTCCTCCAACGTCGAACAAAAGACGATGTGGTTCACTCAGTTAATGACTCATTCACAAATATGGACAGACTAAGATTGCTCAAAATTTGGAATGTGAAGTTTGCTGGAAACATCAATTATCTTTCTAAGAAGTTACAGTTTCTGGAATGGGACGAATGTCCTTTAGACTCTTTCCCGTCAGACTTTCAACCGGATAACCTTGTTGAACTTCAGATGCATTCAAGCTGCCTCAAACAATTATGGAGAGGAAAAAAAGGATGGAGAATGCTACGGCTTATTGATATGAGTAGTTCAGAATACTTGATGTCGACTCCAGATTTCACTGAGGTTCCATTTCTTGAGATATTGGTGCTTCAAGATTGTACGAGGTTGGTTGAGGTTCACCCTTCTCTTGGAGTCCTCAAGAAACTTGTCTCTTTAAATATGAGAAATTGCAAGTCAGTTGAGAGCATTCCACCTTTCATTAGTTTGGAGTCCCTTAAAACTTTGAATCTTTCACTCTGTTCAAAACTCAAGAAGTTTCCTGAAATTGAAGGAAATATGAAAAGCTTGCTGGAGCTTCATTTAGATGGGACTAGTATTGAGGAATTGCCTCTATCAGTTGAAGGTTTGACTGGTCTGACCACATTAAATTTAACAGATTGTAACAACCTTTTGCGTCTCCCCAGTACCATTCAGTCCTTGACATCTTTGAAAAGTCTCATTCTAACAGGTTGTTCAAAACTTGATGAGATAAGTGAGAATCTGAATCATGTGGAATGCCTGGAGAAGCTTAATATAGGCGGAACTGCAATAAGAGATTTATCATTCCTTGTAGGTATGAAGAATTTGAATGGTCTATACTGCCGAGGATGTAAAGGTCTAGCTTTAGGATCATGGAAAGGTCATTCTGAAAGTTTGTTGTTTCCTGCTCTATTATCCAGTTTGACTTCTTTGGTAGAGCTAGATTTAAGGGATTGCAATCTGGTGGATGGAACCATTCCCAATGATCTTAGCAGCTTAATCTCTTTAAGAGAATTAGGTTTAGGTGGTAATAGTTTTGTTCGATTACCTGAAAGTATCTCTCAACTCTCAAAGCTCGAGAAATTAGAGTTGAGCAATTGTAAACAGCTTCAGTTGGTGCCCAAGCTTCCATCAAGTGCTCGACTGGTGCTTGCAGAAGATTGTACTTCACTGATAGATTTCCCAAATCAAATCAAATTATTGCCTTCAATTGAGTCTGGGATGACTACTATCAATTCACTCAGTTCTGCAAGTAGTTCAGCACTTAGAGACTCACGTGGTTTGAGTACGTGGAAATCTTCAGCAAATGCCCAACCTTCTGAGGTGCTTTTGACACATGAGCATACAGAGTGGAAACGAGTTGATCGAGTGTCACTGCCACTTCCAATGCTTATGAAAGACATTGAACTTTCTGATCCATCATCAAAACCCAGTGATATCATTTATTTTGATAATGAAATTCCAGAGTGGTTCAGCACTATATCTACCACCAATCCTATATCAATCTCTATACCTCCAGATCTGACAGATGATGACAAGTGGAAGGGGGTTACAATGTGTGCTGTTTTTGCAGTCAAGGGACATACTGTTCTCTCTGTTATTGAATCGGATTTAGAACTTTCAAACTACTTCTATCAGTGCACAATGGAAACAGATGTTTTTCGTATGAAACCATTCATCTTTGACGGGGAAGAAATCCGCCACCTTCGATCAAGTTCTCATCTGGTTTTGATTTCCTATGTAGCTAGATGGCAGTTTCCAAAGTGGGAGTTAAATCAATCAACCACGGTGGCGGCTAAATTTGAAACCAACAATCCATTCATGGAGGTCCAGAAATGTGGGATTCGTCTAGTTTATGAGCAAGATGCTGGATGGTATACCAGACTATTCCCTGGGGTTAATGCAATTTGTCTAAATGAAGTGATGGATGCTATGCTGAAAACTGGCTGGCTTAGTCAAGTGGATCAATATTTGAAATGGGAGAAAATTATTCCCCCCATCGAAGAAGACTCCAGTACAGTTCTGCTAAGGAAGAACATCGAGTCTGTTCTTCCTCGATACCTTGAGGCATTGAACCATTATCCTGCAACATACAAATTTAACCTCAGAGGAAGCCCGTCGTGGTTTGAGTCCTTTTTCACTGATACAAGGTCAGGGTCGACTCTAGTGTCAATTAAGCTGCCTCAAAATCTACACAAGAGTAAGAAGTGGATGGCATTTGCAATATGCGCATCAATTGCAGAGGAACAGCAAGAGGACTATCATGTTTATTTTAAACTCAAAACGGGTGAATACAATGAGGATCTGTCCCGAGAACTTGAGTACATTGTGGCAATGTCGTCACAAGCAAATCATTATCAACTTTTGGTTGTGTACATACCGCGGGCATTGATTCCCGAAGTCTTGCTCTCTCGGACATCAACAACTGAAATGCACATTTCCTTCAGAGTCGATACTCCTGGTGCAGAAGTTCAAAGAAGTTGTGGCCGTATTGTTTACCAGGAAGATTTAGAAGGGTTTGTTGAAACAATCGTCCGGTGCATGCAAAGAGAAGATACTCTTGAACTTTATGATAAATGGGTGGTAGAAGATTGGATAGACTTGATAGGATTGCAAGGGGGTCATTTATGTACTCCATACAAATGCAAATCCATAGCAGGACGGGAAAGGAAGTTTGAATTGCTCTCTCAGAAATATAGGAATCGGGACTGGGCTATGTCTCCTCCTTATATTTGCGTCTTCTTTGGAGCTGAAATTTCCAAGTGGAATTGGTTCATGCACTTCAATGAGGGAAACACCGCAAAAATCCAACTGCCTCCTAATTTGTTCTATGATGCTAATTGGATGGGGATTGCTGTATGTGGTTTATGTTGGGCGTCCCACGAAGATTCAAACATTATACTAGACGGTAGTCCTGACACAGAGTATAATGTACTCAATTTGTGTCTGGACTCTGATGTCGGTTCGGACGGCTTATTTGATGTCAAATTACAGAAACTTTTTGGGAAACTCAAGCCATGGGAATTTGAAACCGATATCACATTTTTCTTCTATATACCTCGTGTTAAATATTTAAAGTTTTGGAGACAATTCAACCTTGCCAGGGTTACTTTTTCATCTTCTAGCCCATGCTTAAGAGTCTATAGTTGTGCCCTTCGTCTAGTCTTTAAGGAGGACGTTGAAGATCTCGTTCGGACACTGACACTCTCCGAAATACCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1678

Amino Acids

190.64

Weight (kDa)

5.61

Isoelectric Point (pI)

48.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 97 - 268 2.2e-55 TIR domain
TIR_2 PF13676 99 - 199 6.1e-16 TIR domain
NB-ARC PF00931 279 - 433 6.5e-18 NB-ARC domain
WHD_ROQ1 PF23282 502 - 570 6.9e-16 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 743 - 882 1.9e-09 Leucine-rich repeat region
LRR_13 PF23286 761 - 849 1.7e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 909 - 984 1.2e-06 Leucine-rich repeat region
C-JID PF20160 1090 - 1228 1e-08 C-JID domain
C-JID PF20160 1528 - 1666 1.4e-06 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 3982, 4772
Acc16I TGCGCA 1 cut(s) 4045
AccB1I GGYRCC 1 cut(s) 2932
AccI GTMKAC 4 cut(s) 1410, 2120, 3692, 3974
AccII CGCG 1 cut(s) 4198
AccIII TCCGGA 2 cut(s) 871, 1600
AciI CCGC 9 cut(s) 44, 325, 646, 2595, 3514, 3608, 4196, 4198, 4614
AclWI GGATC 8 cut(s) 181, 575, 618, 1181, 2692, 3224, 3786, 4125
AcoI YGGCCR 1 cut(s) 4300
AcuI CTGAAG 9 cut(s) 183, 1250, 1278, 1343, 1740, 2006, 2909, 3099, 4243
AcvI CACGTG 1 cut(s) 3092
AcyI GRCGYC 1 cut(s) 4691
AfiI CCNNNNNNNGG 4 cut(s) 275, 1600, 2204, 4918
AflII CTTAAG 1 cut(s) 4948
AhdI GACNNNNNGTC 1 cut(s) 554
AhlI ACTAGT 1 cut(s) 2387
AjnI CCWGG 8 cut(s) 1162, 1623, 1726, 2574, 3706, 4273, 4314, 4916
AjuI GAANNNNNNNTTGG 4 cut(s) 1505, 1537, 3617, 3649
AloI GAACNNNNNNTCC 2 cut(s) 3387, 3419
Alw21I GWGCWC 2 cut(s) 2956, 3458
Alw26I GTCTC 8 cut(s) 163, 381, 2230, 2477, 2516, 3078, 4539, 4894
Alw44I GTGCAC 1 cut(s) 3454
AlwI GGATC 8 cut(s) 181, 575, 618, 1181, 2692, 3224, 3786, 4125
AlwNI CAGNNNCTG 1 cut(s) 1954
Ama87I CYCGRG 3 cut(s) 401, 2891, 4125
Aor13HI TCCGGA 2 cut(s) 871, 1600
AoxI GGCC 2 cut(s) 1357, 4300
ApaLI GTGCAC 1 cut(s) 3454
ApeKI GCWGC 4 cut(s) 972, 2037, 2808, 3994
ArsI GACNNNNNNTTYG 2 cut(s) 4775, 4807
AseI ATTAAT 1 cut(s) 1467
Asp700I GAANNNNTTC 4 cut(s) 1263, 1317, 3488, 4586
AspLEI GCGC 1 cut(s) 4046
AspS9I GGNCC 4 cut(s) 422, 1114, 1160, 3643
AsuHPI GGTGA 5 cut(s) 769, 824, 1249, 2186, 4115
AsuII TTCGAA 1 cut(s) 1043
AvaI CYCGRG 3 cut(s) 401, 2891, 4125
AvaII GGWCC 4 cut(s) 422, 1114, 1160, 3643
BaeGI GKGCMC 3 cut(s) 2937, 3458, 4969
BaeI ACNNNNGTAYC 2 cut(s) 1670, 1703
BanI GGYRCC 1 cut(s) 2932
BauI CACGAG 1 cut(s) 4875
BbrPI CACGTG 1 cut(s) 3092
BbsI GAAGAC 4 cut(s) 24, 604, 3828, 4546
Bbv12I GWGCWC 2 cut(s) 2956, 3458
BbvI GCAGC 4 cut(s) 984, 2024, 2820, 3981
BceAI ACGGC 3 cut(s) 2099, 4287, 4801
BciT130I CCWGG 8 cut(s) 1164, 1625, 1728, 2576, 3708, 4275, 4316, 4918
BclI TGATCA 1 cut(s) 1777
BcoDI GTCTC 8 cut(s) 163, 381, 2230, 2477, 2516, 3078, 4539, 4894
BcuI ACTAGT 1 cut(s) 2387
BfmI CTRYAG 2 cut(s) 1797, 4957
BfrI CTTAAG 1 cut(s) 4948
BglII AGATCT 2 cut(s) 3334, 4999
BisI GCNGC 5 cut(s) 973, 2038, 2809, 3609, 3995
BlsI GCNGC 5 cut(s) 974, 2039, 2810, 3610, 3996
Bme1390I CCNGG 8 cut(s) 1164, 1625, 1728, 2576, 3708, 4275, 4316, 4918
Bme18I GGWCC 4 cut(s) 422, 1114, 1160, 3643
BmeRI GACNNNNNGTC 1 cut(s) 554
BmeT110I CYCGRG 3 cut(s) 401, 2891, 4125
BmgT120I GGNCC 4 cut(s) 422, 1114, 1160, 3643
BmiI GGNNCC 3 cut(s) 2142, 2788, 2934
BmrFI CCNGG 8 cut(s) 1164, 1625, 1728, 2576, 3708, 4275, 4316, 4918
BmrI ACTGGG 4 cut(s) 557, 2472, 3240, 4534
BmuI ACTGGG 4 cut(s) 557, 2472, 3240, 4534
BoxI GACNNNNGTC 2 cut(s) 1968, 4725
BpiI GAAGAC 4 cut(s) 24, 604, 3828, 4546
BpmI CTGGAG 5 cut(s) 2109, 2387, 2597, 3315, 3811
Bpu10I CCTNAGC 1 cut(s) 3842
Bpu14I TTCGAA 1 cut(s) 1043
BpuEI CTTGAG 7 cut(s) 1001, 2171, 2198, 2309, 3899, 4154, 4811
Bsa29I ATCGAT 1 cut(s) 416
BsaAI YACGTR 3 cut(s) 288, 3092, 3105
BsaBI GATNNNNATC 1 cut(s) 2772
BsaHI GRCGYC 1 cut(s) 4691
BsaI GGTCTC 1 cut(s) 163
BsaWI WCCGGW 5 cut(s) 275, 871, 1600, 2002, 4350
BsaXI ACNNNNNCTCC 4 cut(s) 1212, 1242, 1605, 1635
Bsc4I CCNNNNNNNGG 4 cut(s) 275, 1600, 2204, 4918
Bse3DI GCAATG 2 cut(s) 1527, 4155
Bse8I GATNNNNATC 1 cut(s) 2772
BseAI TCCGGA 2 cut(s) 871, 1600
BseBI CCWGG 8 cut(s) 1164, 1625, 1728, 2576, 3708, 4275, 4316, 4918
BseCI ATCGAT 1 cut(s) 416
BseJI GATNNNNATC 1 cut(s) 2772
BseLI CCNNNNNNNGG 4 cut(s) 275, 1600, 2204, 4918
BseMI GCAATG 2 cut(s) 1527, 4155
BseRI GAGGAG 2 cut(s) 1805, 4527
BseSI GKGCMC 3 cut(s) 2937, 3458, 4969
BseXI GCAGC 4 cut(s) 984, 2024, 2820, 3981
BsgI GTGCAG 3 cut(s) 1035, 1230, 4299
Bsh1236I CGCG 1 cut(s) 4198
BshFI GGCC 2 cut(s) 1359, 4302
BshNI GGYRCC 1 cut(s) 2932
BshVI ATCGAT 1 cut(s) 416
BsiHKAI GWGCWC 2 cut(s) 2956, 3458
BsiHKCI CYCGRG 3 cut(s) 401, 2891, 4125
BsiSI CCGG 6 cut(s) 16, 276, 872, 1601, 2003, 4351
BsiWI CGTACG 1 cut(s) 288
BslFI GGGAC 8 cut(s) 561, 1976, 2272, 2398, 3408, 4108, 4535, 4678
BslI CCNNNNNNNGG 4 cut(s) 275, 1600, 2204, 4918
BsmAI GTCTC 8 cut(s) 163, 381, 2230, 2477, 2516, 3078, 4539, 4894
BsmBI CGTCTC 2 cut(s) 381, 2477
BsmFI GGGAC 8 cut(s) 561, 1976, 2272, 2398, 3408, 4108, 4535, 4678
BsmI GAATGC 4 cut(s) 2029, 2082, 2262, 2576
BsnI GGCC 2 cut(s) 1359, 4302
Bso31I GGTCTC 1 cut(s) 163
BsoBI CYCGRG 3 cut(s) 401, 2891, 4125
Bsp119I TTCGAA 1 cut(s) 1043
Bsp1286I GDGCHC 4 cut(s) 2937, 2956, 3458, 4969
Bsp13I TCCGGA 2 cut(s) 871, 1600
Bsp1407I TGTACA 2 cut(s) 1612, 4188
Bsp19I CCATGG 2 cut(s) 543, 4832
BspACI CCGC 9 cut(s) 44, 325, 646, 2595, 3514, 3608, 4196, 4198, 4614
BspANI GGCC 2 cut(s) 1359, 4302
BspDI ATCGAT 1 cut(s) 416
BspEI TCCGGA 2 cut(s) 871, 1600
BspFNI CGCG 1 cut(s) 4198
BspLI GGNNCC 3 cut(s) 2142, 2788, 2934
BspMAI CTGCAG 1 cut(s) 1801
BspPI GGATC 8 cut(s) 181, 575, 618, 1181, 2692, 3224, 3786, 4125
BspQI GCTCTTC 2 cut(s) 1269, 1716
BspT104I TTCGAA 1 cut(s) 1043
BspT107I GGYRCC 1 cut(s) 2932
BspTI CTTAAG 1 cut(s) 4948
BspTNI GGTCTC 1 cut(s) 163
BsrDI GCAATG 2 cut(s) 1527, 4155
BsrGI TGTACA 2 cut(s) 1612, 4188
BssNAI GTATAC 1 cut(s) 3693
BssNI GRCGYC 1 cut(s) 4691
BssSI CACGAG 1 cut(s) 4875
BssT1I CCWWGG 2 cut(s) 543, 4832
Bst1107I GTATAC 1 cut(s) 3693
Bst2BI CACGAG 1 cut(s) 4875
Bst2UI CCWGG 8 cut(s) 1164, 1625, 1728, 2576, 3708, 4275, 4316, 4918
Bst6I CTCTTC 2 cut(s) 1269, 1716
BstACI GRCGYC 1 cut(s) 4691
BstAFI CTTAAG 1 cut(s) 4948
BstAPI GCANNNNNTGC 2 cut(s) 1796, 4052
BstAUI TGTACA 2 cut(s) 1612, 4188
BstBAI YACGTR 3 cut(s) 288, 3092, 3105
BstBI TTCGAA 1 cut(s) 1043
BstC8I GCNNGC 6 cut(s) 44, 2363, 2967, 3764, 4200, 4358
BstDSI CCRYGG 4 cut(s) 543, 3600, 4196, 4832
BstFNI CGCG 1 cut(s) 4198
BstHHI GCGC 1 cut(s) 4046
BstMAI GTCTC 8 cut(s) 163, 381, 2230, 2477, 2516, 3078, 4539, 4894
BstMWI GCNNNNNNNGC 7 cut(s) 494, 524, 643, 1796, 3383, 4043, 4052
BstNI CCWGG 8 cut(s) 1164, 1625, 1728, 2576, 3708, 4275, 4316, 4918
BstNSI RCATGY 2 cut(s) 1536, 4360
BstPAI GACNNNNGTC 2 cut(s) 1968, 4725
BstSCI CCNGG 8 cut(s) 1162, 1623, 1726, 2574, 3706, 4273, 4314, 4916
BstSFI CTRYAG 2 cut(s) 1797, 4957
BstSLI GKGCMC 3 cut(s) 2937, 3458, 4969
BstUI CGCG 1 cut(s) 4198
BstV1I GCAGC 4 cut(s) 984, 2024, 2820, 3981
BstV2I GAAGAC 4 cut(s) 24, 604, 3828, 4546
BstX2I RGATCY 3 cut(s) 3334, 4117, 4999
BstXI CCANNNNNNTGG 2 cut(s) 1307, 3653
BstYI RGATCY 3 cut(s) 3334, 4117, 4999
BstZ17I GTATAC 1 cut(s) 3693
Bsu15I ATCGAT 1 cut(s) 416
BsuRI GGCC 2 cut(s) 1359, 4302
BsuTUI ATCGAT 1 cut(s) 416
BtgI CCRYGG 4 cut(s) 543, 3600, 4196, 4832
BtsI GCAGTG 3 cut(s) 1023, 1794, 3188
Cac8I GCNNGC 6 cut(s) 44, 2363, 2967, 3764, 4200, 4358
CaiI CAGNNNCTG 1 cut(s) 1954
CfoI GCGC 1 cut(s) 4046
Cfr13I GGNCC 4 cut(s) 422, 1114, 1160, 3643
Cfr42I CCGCGG 1 cut(s) 4199
ClaI ATCGAT 1 cut(s) 416
CseI GACGC 4 cut(s) 695, 2459, 4540, 4680
CspCI CAANNNNNGTGG 2 cut(s) 3585, 3620
DraI TTTAAA 4 cut(s) 1543, 2232, 4090, 4890
DrdI GACNNNNNNGTC 2 cut(s) 3982, 4772
DriI GACNNNNNGTC 1 cut(s) 554
DseDI GACNNNNNNGTC 2 cut(s) 3982, 4772
EaeI YGGCCR 1 cut(s) 4300
Eam1104I CTCTTC 2 cut(s) 1269, 1716
Eam1105I GACNNNNNGTC 1 cut(s) 554
EarI CTCTTC 2 cut(s) 1269, 1716
EciI GGCGGA 2 cut(s) 2610, 3503
Eco130I CCWWGG 2 cut(s) 543, 4832
Eco31I GGTCTC 1 cut(s) 163
Eco32I GATATC 2 cut(s) 3253, 4852
Eco47I GGWCC 4 cut(s) 422, 1114, 1160, 3643
Eco57I CTGAAG 9 cut(s) 183, 1250, 1278, 1343, 1740, 2006, 2909, 3099, 4243
Eco72I CACGTG 1 cut(s) 3092
Eco88I CYCGRG 3 cut(s) 401, 2891, 4125
EcoRII CCWGG 8 cut(s) 1162, 1623, 1726, 2574, 3706, 4273, 4314, 4916
EcoRV GATATC 2 cut(s) 3253, 4852
EcoT14I CCWWGG 2 cut(s) 543, 4832
EcoT22I ATGCAT 2 cut(s) 1671, 2031
ErhI CCWWGG 2 cut(s) 543, 4832
Esp3I CGTCTC 2 cut(s) 381, 2477
FalI AAGNNNNNCTT 4 cut(s) 1020, 1052, 4359, 4391
FaqI GGGAC 8 cut(s) 561, 1976, 2272, 2398, 3408, 4108, 4535, 4678
FauI CCCGC 3 cut(s) 51, 332, 4191
FbaI TGATCA 1 cut(s) 1777
FblI GTMKAC 4 cut(s) 1410, 2120, 3692, 3974
Fnu4HI GCNGC 5 cut(s) 973, 2038, 2809, 3609, 3995
Fsp4HI GCNGC 5 cut(s) 973, 2038, 2809, 3609, 3995
FspAI RTGCGCAY 1 cut(s) 4045
FspI TGCGCA 1 cut(s) 4045
GlaI GCGC 1 cut(s) 4045
GluI GCNGC 5 cut(s) 973, 2038, 2809, 3609, 3995
GsuI CTGGAG 5 cut(s) 2109, 2387, 2597, 3315, 3811
HaeIII GGCC 2 cut(s) 1359, 4302
HapII CCGG 6 cut(s) 16, 276, 872, 1601, 2003, 4351
HgaI GACGC 4 cut(s) 695, 2459, 4540, 4680
HhaI GCGC 1 cut(s) 4046
Hin1I GRCGYC 1 cut(s) 4691
Hin6I GCGC 1 cut(s) 4044
HinP1I GCGC 1 cut(s) 4044
HincII GTYRAC 2 cut(s) 2121, 3975
HindII GTYRAC 2 cut(s) 2121, 3975
HindIII AAGCTT 3 cut(s) 2359, 2582, 2939
HpaII CCGG 6 cut(s) 16, 276, 872, 1601, 2003, 4351
HphI GGTGA 5 cut(s) 769, 824, 1249, 2186, 4115
Hpy99I CGWCG 4 cut(s) 256, 259, 1827, 3937
HpyCH4IV ACGT 6 cut(s) 287, 1735, 1822, 3091, 3104, 4992
HpyF10VI GCNNNNNNNGC 7 cut(s) 494, 524, 643, 1796, 3383, 4043, 4052
HpySE526I ACGT 6 cut(s) 287, 1735, 1822, 3091, 3104, 4992
Hsp92I GRCGYC 1 cut(s) 4691
HspAI GCGC 1 cut(s) 4044
Kpn2I TCCGGA 2 cut(s) 871, 1600
Ksp22I TGATCA 1 cut(s) 1777
KspI CCGCGG 1 cut(s) 4199
LguI GCTCTTC 2 cut(s) 1269, 1716
LmnI GCTCC 2 cut(s) 2368, 4562
Lsp1109I GCAGC 4 cut(s) 984, 2024, 2820, 3981
MaeII ACGT 6 cut(s) 287, 1735, 1822, 3091, 3104, 4992
MaeIII GTNAC 8 cut(s) 175, 202, 1944, 2456, 3186, 3364, 4155, 4921
MfeI CAATTG 4 cut(s) 1127, 2911, 3024, 4050
MflI RGATCY 3 cut(s) 3334, 4117, 4999
MhlI GDGCHC 4 cut(s) 2937, 2956, 3458, 4969
MmeI TCCRAC 3 cut(s) 1288, 1843, 4648
Mph1103I ATGCAT 2 cut(s) 1671, 2031
MroI TCCGGA 2 cut(s) 871, 1600
MroXI GAANNNNTTC 4 cut(s) 1263, 1317, 3488, 4586
MslI CAYNNNNRTG 5 cut(s) 837, 1871, 2379, 4456, 4599
MspA1I CMGCKG 2 cut(s) 1303, 4198
MspCI CTTAAG 1 cut(s) 4948
MspI CCGG 6 cut(s) 16, 276, 872, 1601, 2003, 4351
MspR9I CCNGG 8 cut(s) 1164, 1625, 1728, 2576, 3708, 4275, 4316, 4918
MunI CAATTG 4 cut(s) 1127, 2911, 3024, 4050
Mva1269I GAATGC 4 cut(s) 2029, 2082, 2262, 2576
MvaI CCWGG 8 cut(s) 1164, 1625, 1728, 2576, 3708, 4275, 4316, 4918
MvnI CGCG 1 cut(s) 4198
MwoI GCNNNNNNNGC 7 cut(s) 494, 524, 643, 1796, 3383, 4043, 4052
NcoI CCATGG 2 cut(s) 543, 4832
NlaIV GGNNCC 3 cut(s) 2142, 2788, 2934
NmeAIII GCCGAG 1 cut(s) 2684
NmuCI GTSAC 2 cut(s) 3186, 4155
NsbI TGCGCA 1 cut(s) 4045
NsiI ATGCAT 2 cut(s) 1671, 2031
NspI RCATGY 2 cut(s) 1536, 4360
NspV TTCGAA 1 cut(s) 1043
PaeI GCATGC 1 cut(s) 4360
PaeR7I CTCGAG 2 cut(s) 401, 2891
PasI CCCWGGG 1 cut(s) 3707
PciSI GCTCTTC 2 cut(s) 1269, 1716
PcsI WCGNNNNNNNCGW 1 cut(s) 3178
PctI GAATGC 4 cut(s) 2029, 2082, 2262, 2576
PdmI GAANNNNTTC 4 cut(s) 1263, 1317, 3488, 4586
Pfl23II CGTACG 1 cut(s) 288
PfoI TCCNGGA 1 cut(s) 1623
PkrI GCNGC 5 cut(s) 974, 2039, 2810, 3610, 3996
PmaCI CACGTG 1 cut(s) 3092
PmlI CACGTG 1 cut(s) 3092
Ppu21I YACGTR 3 cut(s) 288, 3092, 3105
PshAI GACNNNNGTC 2 cut(s) 1968, 4725
PshBI ATTAAT 1 cut(s) 1467
Psp6I CCWGG 8 cut(s) 1162, 1623, 1726, 2574, 3706, 4273, 4314, 4916
PspCI CACGTG 1 cut(s) 3092
PspGI CCWGG 8 cut(s) 1162, 1623, 1726, 2574, 3706, 4273, 4314, 4916
PspLI CGTACG 1 cut(s) 288
PspN4I GGNNCC 3 cut(s) 2142, 2788, 2934
PspPI GGNCC 4 cut(s) 422, 1114, 1160, 3643
PsrI GAACNNNNNNTAC 4 cut(s) 89, 121, 281, 313
PstI CTGCAG 1 cut(s) 1801
PstNI CAGNNNCTG 1 cut(s) 1954
PsuI RGATCY 3 cut(s) 3334, 4117, 4999
PvuII CAGCTG 1 cut(s) 1303
RseI CAYNNNNRTG 5 cut(s) 837, 1871, 2379, 4456, 4599
SacII CCGCGG 1 cut(s) 4199
SalI GTCGAC 2 cut(s) 2119, 3973
SapI GCTCTTC 2 cut(s) 1269, 1716
SatI GCNGC 5 cut(s) 973, 2038, 2809, 3609, 3995
Sau96I GGNCC 4 cut(s) 422, 1114, 1160, 3643
ScrFI CCNGG 8 cut(s) 1164, 1625, 1728, 2576, 3708, 4275, 4316, 4918
SduI GDGCHC 4 cut(s) 2937, 2956, 3458, 4969
SfcI CTRYAG 2 cut(s) 1797, 4957
Sfr274I CTCGAG 2 cut(s) 401, 2891
Sfr303I CCGCGG 1 cut(s) 4199
SfuI TTCGAA 1 cut(s) 1043
SgrBI CCGCGG 1 cut(s) 4199
SinI GGWCC 4 cut(s) 422, 1114, 1160, 3643
SlaI CTCGAG 2 cut(s) 401, 2891
SmiMI CAYNNNNRTG 5 cut(s) 837, 1871, 2379, 4456, 4599
SpeI ACTAGT 1 cut(s) 2387
SphI GCATGC 1 cut(s) 4360
SsiI CCGC 9 cut(s) 44, 325, 646, 2595, 3514, 3608, 4196, 4198, 4614
SspI AATATT 3 cut(s) 1575, 3785, 4886
StyD4I CCNGG 8 cut(s) 1162, 1623, 1726, 2574, 3706, 4273, 4314, 4916
StyI CCWWGG 2 cut(s) 543, 4832
TaiI ACGT 6 cut(s) 290, 1738, 1825, 3094, 3107, 4995
TatI WGTACW 8 cut(s) 1079, 1612, 2978, 3830, 4137, 4188, 4445, 4747
TauI GCSGC 1 cut(s) 3611
TseFI GTSAC 2 cut(s) 3186, 4155
TseI GCWGC 4 cut(s) 972, 2037, 2808, 3994
Tsp45I GTSAC 2 cut(s) 3186, 4155
TspGWI ACGGA 1 cut(s) 1694
Vha464I CTTAAG 1 cut(s) 4948
VneI GTGCAC 1 cut(s) 3454
VpaK11BI GGWCC 4 cut(s) 422, 1114, 1160, 3643
VspI ATTAAT 1 cut(s) 1467
XbaI TCTAGA 1 cut(s) 1187
XceI RCATGY 2 cut(s) 1536, 4360
XhoI CTCGAG 2 cut(s) 401, 2891
XmiI GTMKAC 4 cut(s) 1410, 2120, 3692, 3974
XmnI GAANNNNTTC 4 cut(s) 1263, 1317, 3488, 4586
Zsp2I ATGCAT 2 cut(s) 1671, 2031
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.