Rorug07G0338000

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000129
Physical Location & Seq
Forward (+)
37133989 .. 37134975
987 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0338000.1

Sequence Viewer

Length: 987 bp
ATGCCGGCTTTTGTGTTCTCCGCCCTTCCACCGGGAAGCGGCGGAGGCAACGTTTCTCTGATGATGCAACGCAAACGAACTAAGAAGGGCGGTGATATCTCCTCTGATCTTGAAGCTCTTCGCATGGAAGATCTTGATTCTTCTACTCAGTCCATTCCGATTCAGCAATCTGATTCAAGCTATGCTAGTAAGTTGAAAAACCCGGTTGATCGATATAGGCAGACGATGATGGAAGAGTTCGAATTCACTGATGAGGATTGCAACTACACTCAAGGCAAGCATGGTCAAAATGTTTCTTTCTCGGAGAAGGTACACAATAAGCTTGACTTTGATTGGCGTTGTGCTGTAGTTGTTAAACTTATGGGTAGACCGAATTCTACAAACACCTTTGATTTCATGTTGAAAGGGTTAAGAAGAAAATGGCAAGTCAAAGGGGGCTGGCACTTCATTGACCTTCCCAATGACTTTTTCATCGTTAAGTTTAATCTGGAAGAAGATATGAATAGTGTGTTGTGTGGAGGACCTTGGATTCTAGCTGGTCAGACTTTGATTGTTAGGAAATGGAGGCCTGATTTTGACCCCTTGAATGAATTTATTGGGAAAATGGCTTTATGGGTTAGAATTTGTGGTTTGCCTGTTAAATATTTCAAGGATTACACTGTTGCTAAAATTGGCAAAATTCTTGGAGATGTTGTTAAGGTTGATCAGGTTACTATTGGCCAATCTTGGGGCAAATTTGCTCGAGTTTGTATTGAAGTTGATCTCAGTAAGCCCCTGCGCCCTTATGTTGAAGTGGAATCTGTTGCCTACCATGTTGTTTATGAAGGAATTTCCATGATATGCTTTGAATGTGGTTGCTTTGGCCACTCTAAGGATAAATGCCCAACCATACATGCCGTGCCAAATGCTAATCCTCAAGCTAGTGAACCTGATAGCAACACTATTGACCAGGTTATTGTCTCCAAGGGAAATTCGGAATCACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

328

Amino Acids

36.97

Weight (kDa)

6.6

Isoelectric Point (pI)

36.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 111 - 194 2.1e-14 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 367
AciI CCGC 4 cut(s) 21, 39, 42, 90
AclI AACGTT 1 cut(s) 51
AcoI YGGCCR 2 cut(s) 718, 862
AcsI RAATTY 8 cut(s) 242, 373, 590, 621, 678, 734, 828, 970
AfaI GTAC 1 cut(s) 312
AfiI CCNNNNNNNGG 4 cut(s) 31, 38, 727, 871
AgsI TTSAA 9 cut(s) 113, 177, 196, 403, 586, 649, 755, 791, 848
AjnI CCWGG 1 cut(s) 948
AjuI GAANNNNNNNTTGG 2 cut(s) 452, 484
AluBI AGCT 5 cut(s) 116, 180, 322, 536, 920
AluI AGCT 5 cut(s) 116, 180, 322, 536, 920
Alw26I GTCTC 1 cut(s) 964
Ama87I CYCGRG 1 cut(s) 741
AoxI GGCC 3 cut(s) 566, 718, 862
ApoI RAATTY 8 cut(s) 242, 373, 590, 621, 678, 734, 828, 970
Asp700I GAANNNNTTC 1 cut(s) 117
AspLEI GCGC 1 cut(s) 780
AspS9I GGNCC 1 cut(s) 521
AsuC2I CCSGG 2 cut(s) 33, 203
AsuHPI GGTGA 1 cut(s) 104
AsuII TTCGAA 1 cut(s) 240
AvaI CYCGRG 1 cut(s) 741
AvaII GGWCC 1 cut(s) 521
BalI TGGCCA 2 cut(s) 720, 864
BccI CCATC 1 cut(s) 223
BceAI ACGGC 1 cut(s) 881
BciT130I CCWGG 1 cut(s) 950
BclI TGATCA 1 cut(s) 703
BcnI CCSGG 2 cut(s) 33, 203
BcoDI GTCTC 1 cut(s) 964
BfaI CTAG 3 cut(s) 186, 533, 921
BfmI CTRYAG 1 cut(s) 345
BglII AGATCT 1 cut(s) 130
BisI GCNGC 1 cut(s) 40
BlsI GCNGC 1 cut(s) 41
Bme1390I CCNGG 3 cut(s) 33, 203, 950
Bme18I GGWCC 1 cut(s) 521
BmeT110I CYCGRG 1 cut(s) 741
BmgT120I GGNCC 1 cut(s) 521
BmrFI CCNGG 3 cut(s) 33, 203, 950
BmsI GCATC 1 cut(s) 54
Bpu14I TTCGAA 1 cut(s) 240
BpuEI CTTGAG 2 cut(s) 255, 900
BpuMI CCSGG 2 cut(s) 33, 203
Bsa29I ATCGAT 1 cut(s) 211
BsaJI CCNNGG 2 cut(s) 524, 963
BsaXI ACNNNNNCTCC 4 cut(s) 36, 66, 678, 708
Bsc4I CCNNNNNNNGG 4 cut(s) 31, 38, 727, 871
Bse118I RCCGGY 1 cut(s) 4
BseBI CCWGG 1 cut(s) 950
BseCI ATCGAT 1 cut(s) 211
BseDI CCNNGG 2 cut(s) 524, 963
BseLI CCNNNNNNNGG 4 cut(s) 31, 38, 727, 871
BseMII CTCAG 2 cut(s) 161, 778
BseRI GAGGAG 1 cut(s) 91
BshFI GGCC 3 cut(s) 568, 720, 864
BshVI ATCGAT 1 cut(s) 211
BsiHKCI CYCGRG 1 cut(s) 741
BsiSI CCGG 3 cut(s) 5, 32, 203
BslI CCNNNNNNNGG 4 cut(s) 31, 38, 727, 871
BsmAI GTCTC 1 cut(s) 964
BsnI GGCC 3 cut(s) 568, 720, 864
BsoBI CYCGRG 1 cut(s) 741
Bsp119I TTCGAA 1 cut(s) 240
Bsp143I GATC 5 cut(s) 106, 130, 208, 703, 760
BspACI CCGC 4 cut(s) 21, 39, 42, 90
BspANI GGCC 3 cut(s) 568, 720, 864
BspCNI CTCAG 2 cut(s) 160, 777
BspDI ATCGAT 1 cut(s) 211
BspQI GCTCTTC 1 cut(s) 123
BspT104I TTCGAA 1 cut(s) 240
BsrFI RCCGGY 1 cut(s) 4
BssAI RCCGGY 1 cut(s) 4
BssECI CCNNGG 2 cut(s) 524, 963
BssMI GATC 5 cut(s) 106, 130, 208, 703, 760
BssT1I CCWWGG 2 cut(s) 524, 963
Bst2UI CCWGG 1 cut(s) 950
Bst4CI ACNGT 1 cut(s) 661
Bst6I CTCTTC 2 cut(s) 123, 228
BstBI TTCGAA 1 cut(s) 240
BstC8I GCNNGC 3 cut(s) 6, 278, 440
BstDEI CTNAG 4 cut(s) 81, 147, 764, 870
BstHHI GCGC 1 cut(s) 780
BstKTI GATC 5 cut(s) 109, 133, 211, 706, 763
BstMAI GTCTC 1 cut(s) 964
BstMBI GATC 5 cut(s) 106, 130, 208, 703, 760
BstMWI GCNNNNNNNGC 1 cut(s) 45
BstNI CCWGG 1 cut(s) 950
BstNSI RCATGY 1 cut(s) 896
BstSCI CCNGG 3 cut(s) 31, 201, 948
BstSFI CTRYAG 1 cut(s) 345
BstX2I RGATCY 1 cut(s) 130
BstYI RGATCY 1 cut(s) 130
Bsu15I ATCGAT 1 cut(s) 211
BsuRI GGCC 3 cut(s) 568, 720, 864
BsuTUI ATCGAT 1 cut(s) 211
BtsIMutI CAGTG 2 cut(s) 246, 657
Cac8I GCNNGC 3 cut(s) 6, 278, 440
CfoI GCGC 1 cut(s) 780
Cfr10I RCCGGY 1 cut(s) 4
Cfr13I GGNCC 1 cut(s) 521
ClaI ATCGAT 1 cut(s) 211
CsiI ACCWGGT 1 cut(s) 948
Csp6I GTAC 1 cut(s) 311
CviAII CATG 6 cut(s) 124, 281, 397, 812, 835, 893
CviQI GTAC 1 cut(s) 311
DdeI CTNAG 4 cut(s) 81, 147, 764, 870
DpnI GATC 5 cut(s) 108, 132, 210, 705, 762
DpnII GATC 5 cut(s) 106, 130, 208, 703, 760
EaeI YGGCCR 2 cut(s) 718, 862
Eam1104I CTCTTC 2 cut(s) 123, 228
EarI CTCTTC 2 cut(s) 123, 228
EciI GGCGGA 2 cut(s) 10, 57
Eco130I CCWWGG 2 cut(s) 524, 963
Eco147I AGGCCT 1 cut(s) 568
Eco32I GATATC 1 cut(s) 97
Eco47I GGWCC 1 cut(s) 521
Eco88I CYCGRG 1 cut(s) 741
EcoO109I RGGNCCY 1 cut(s) 521
EcoRI GAATTC 2 cut(s) 242, 373
EcoRII CCWGG 1 cut(s) 948
EcoRV GATATC 1 cut(s) 97
EcoT14I CCWWGG 2 cut(s) 524, 963
ErhI CCWWGG 2 cut(s) 524, 963
FaeI CATG 6 cut(s) 127, 284, 400, 815, 838, 896
FalI AAGNNNNNCTT 2 cut(s) 311, 343
FatI CATG 6 cut(s) 123, 280, 396, 811, 834, 892
FbaI TGATCA 1 cut(s) 703
FblI GTMKAC 1 cut(s) 367
Fnu4HI GCNGC 1 cut(s) 40
Fsp4HI GCNGC 1 cut(s) 40
FspBI CTAG 3 cut(s) 186, 533, 921
GlaI GCGC 1 cut(s) 779
GluI GCNGC 1 cut(s) 40
HaeIII GGCC 3 cut(s) 568, 720, 864
HapII CCGG 3 cut(s) 5, 32, 203
HhaI GCGC 1 cut(s) 780
Hin1II CATG 6 cut(s) 127, 284, 400, 815, 838, 896
Hin6I GCGC 1 cut(s) 778
HinP1I GCGC 1 cut(s) 778
HindIII AAGCTT 1 cut(s) 320
HinfI GANTC 6 cut(s) 137, 160, 173, 529, 797, 977
HpaII CCGG 3 cut(s) 5, 32, 203
HphI GGTGA 1 cut(s) 104
Hpy166II GTNNAC 3 cut(s) 313, 368, 926
Hpy188I TCNGA 7 cut(s) 60, 106, 159, 172, 304, 543, 976
Hpy188III TCNNGA 3 cut(s) 110, 134, 488
Hpy8I GTNNAC 3 cut(s) 313, 368, 926
HpyAV CCTTC 5 cut(s) 35, 79, 301, 464, 818
HpyCH4III ACNGT 1 cut(s) 661
HpyCH4IV ACGT 1 cut(s) 51
HpyCH4V TGCA 2 cut(s) 67, 261
HpyF10VI GCNNNNNNNGC 1 cut(s) 45
HpyF3I CTNAG 4 cut(s) 81, 147, 764, 870
HpySE526I ACGT 1 cut(s) 51
Hsp92II CATG 6 cut(s) 127, 284, 400, 815, 838, 896
HspAI GCGC 1 cut(s) 778
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Ksp22I TGATCA 1 cut(s) 703
Kzo9I GATC 5 cut(s) 106, 130, 208, 703, 760
LguI GCTCTTC 1 cut(s) 123
LweI GCATC 1 cut(s) 54
MabI ACCWGGT 1 cut(s) 948
MaeI CTAG 3 cut(s) 186, 533, 921
MaeII ACGT 1 cut(s) 51
MaeIII GTNAC 1 cut(s) 709
MalI GATC 5 cut(s) 108, 132, 210, 705, 762
MboI GATC 5 cut(s) 106, 130, 208, 703, 760
MboII GAAGA 7 cut(s) 110, 132, 140, 245, 426, 503, 506
MflI RGATCY 1 cut(s) 130
MlsI TGGCCA 2 cut(s) 720, 864
MluCI AATT 9 cut(s) 242, 373, 590, 621, 669, 678, 734, 828, 970
MluNI TGGCCA 2 cut(s) 720, 864
MnlI CCTC 6 cut(s) 38, 112, 247, 512, 558, 924
Mox20I TGGCCA 2 cut(s) 720, 864
MroNI GCCGGC 1 cut(s) 4
MroXI GAANNNNTTC 1 cut(s) 117
MscI TGGCCA 2 cut(s) 720, 864
MseI TTAA 6 cut(s) 354, 410, 477, 483, 639, 696
Msp20I TGGCCA 2 cut(s) 720, 864
MspI CCGG 3 cut(s) 5, 32, 203
MspR9I CCNGG 3 cut(s) 33, 203, 950
MvaI CCWGG 1 cut(s) 950
MwoI GCNNNNNNNGC 1 cut(s) 45
NaeI GCCGGC 1 cut(s) 6
NciI CCSGG 2 cut(s) 33, 203
NdeII GATC 5 cut(s) 106, 130, 208, 703, 760
NgoMIV GCCGGC 1 cut(s) 4
NlaIII CATG 6 cut(s) 127, 284, 400, 815, 838, 896
NspI RCATGY 1 cut(s) 896
NspV TTCGAA 1 cut(s) 240
PaeR7I CTCGAG 1 cut(s) 741
PceI AGGCCT 1 cut(s) 568
PciSI GCTCTTC 1 cut(s) 123
PdiI GCCGGC 1 cut(s) 6
PdmI GAANNNNTTC 1 cut(s) 117
PfeI GAWTC 6 cut(s) 137, 160, 173, 529, 797, 977
PkrI GCNGC 1 cut(s) 41
PpuMI RGGWCCY 1 cut(s) 521
Psp1406I AACGTT 1 cut(s) 51
Psp5II RGGWCCY 1 cut(s) 521
Psp6I CCWGG 1 cut(s) 948
PspGI CCWGG 1 cut(s) 948
PspPI GGNCC 1 cut(s) 521
PspPPI RGGWCCY 1 cut(s) 521
PspXI VCTCGAGB 1 cut(s) 741
PsuI RGATCY 1 cut(s) 130
RsaI GTAC 1 cut(s) 312
RsaNI GTAC 1 cut(s) 311
SapI GCTCTTC 1 cut(s) 123
SaqAI TTAA 6 cut(s) 354, 410, 477, 483, 639, 696
SatI GCNGC 1 cut(s) 40
Sau3AI GATC 5 cut(s) 106, 130, 208, 703, 760
Sau96I GGNCC 1 cut(s) 521
ScrFI CCNGG 3 cut(s) 33, 203, 950
SexAI ACCWGGT 1 cut(s) 948
SfaNI GCATC 1 cut(s) 54
SfcI CTRYAG 1 cut(s) 345
Sfr274I CTCGAG 1 cut(s) 741
SfuI TTCGAA 1 cut(s) 240
SinI GGWCC 1 cut(s) 521
SlaI CTCGAG 1 cut(s) 741
SmlI CTYRAG 3 cut(s) 270, 741, 915
SmoI CTYRAG 3 cut(s) 270, 741, 915
Sse9I AATT 9 cut(s) 242, 373, 590, 621, 669, 678, 734, 828, 970
SseBI AGGCCT 1 cut(s) 568
SsiI CCGC 4 cut(s) 21, 39, 42, 90
SspI AATATT 1 cut(s) 644
SspMI CTAG 3 cut(s) 186, 533, 921
StuI AGGCCT 1 cut(s) 568
StyD4I CCNGG 3 cut(s) 31, 201, 948
StyI CCWWGG 2 cut(s) 524, 963
TaaI ACNGT 1 cut(s) 661
TaiI ACGT 1 cut(s) 54
TaqI TCGA 3 cut(s) 211, 240, 742
TaqII GACCGA 1 cut(s) 385
TasI AATT 9 cut(s) 242, 373, 590, 621, 669, 678, 734, 828, 970
TauI GCSGC 1 cut(s) 42
TfiI GAWTC 6 cut(s) 137, 160, 173, 529, 797, 977
Tru1I TTAA 6 cut(s) 354, 410, 477, 483, 639, 696
Tru9I TTAA 6 cut(s) 354, 410, 477, 483, 639, 696
TscAI CASTG 2 cut(s) 253, 664
TspDTI ATGAA 6 cut(s) 385, 436, 460, 515, 603, 837
TspRI CASTG 2 cut(s) 253, 664
VpaK11BI GGWCC 1 cut(s) 521
XapI RAATTY 8 cut(s) 242, 373, 590, 621, 678, 734, 828, 970
XceI RCATGY 1 cut(s) 896
XhoI CTCGAG 1 cut(s) 741
XmiI GTMKAC 1 cut(s) 367
XmnI GAANNNNTTC 1 cut(s) 117
XspI CTAG 3 cut(s) 186, 533, 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.