RchiOBHm_Chr7g0240641

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000129
Physical Location & Seq
Forward (+)
66611595 .. 66617806
6212 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21566

Sequence Viewer

Length: 4251 bp
ATGGATTCTCACCTGAATGAAATGCTTTCATACTTGGACATTGGGTGTCCGGATGTTCGTATCATAGGGATTTGTGGGATGGGTGGTATCGGTAAGACAACTATTGCACAAGTGGTTTTTGAAAGGGTACAAGCTCAGTTTGATGGTTGCAGCTTTCTTGAGAATGTTAGAGAGGTAATTGAAAAGCAAGGTGCAGTGCATTTACAAGAGCAACTTCTTTCGAATTTGCTGAGATTTAGTGTAAATGTACAGAACACTAAAATGGGAAAAAATATAATGAGGCAGAGGCTATGTACTAAAATGGTTCTTCTCATTCTTGATGATGTGGACCAAGAAGAACAATTGGAAGCATTGTGTGATAGGGAATCGTTTGGTCCAGGGAGTAGGATCATCATAACTTCTAGAGATGAACATTTGCTCAGTGCAGTTGAAGGAGATAAAGTATATAAGGTGAATCCATTAACTGATGCTGAAGCTCTTCAGCTTTTCAGTATGAAAGCCTTTAAGAAAGACCAGCTGGTTGGAGAAGAAATTCTGAAGCTATCCAAAGAGTTTTTGAAATATGCTAATGGCCTTCCACTAGCTATTAAAGTTTTGGGTTCTTCTGTTAAGGGTAGAAGTGTAGACTTATGGTTAAGTGCATTGGATAGACTTAAAGAAAATCCTGAAAAAAAAATTATTAATGTTCTCAAAGTTAGTTTTGATGGATTAGAACCAAGAGAAAAGAAGATTTTTTTGGACATTGCATGTTTCTTTAAAGGGGAGAGCATAGATCGTGTAAGAAGAATATTACAAGGTAGTGATGCCCACTGTCCGGACTTTGATGTACAAGTTCTCCTGGACAAATCTATGATAACTGTATTTGGAAAAAGATTGTGGATGCATGATTTGATACAACAGTTAGGTTGGGAAGTTGTCCGTCAAGAATGTCCTGAAGAGCCTGGAAAACGTAGTCGATTGTGGCTTCCAAATGAGATCATTGATGTTCTTGATCAGAATAAGGCAACCACTGCAGTTCAAAGCATACTCCTCCAACGTCGAACAAAAGACGATGTGGTTCACTCAGTTAATGACTCATTCACAAATATGGACAGACTAAGATTGCTCAAAATTTGGAACGTGAAGTTTGCTGGAAACATCAATTATCTTTCTAAGAAGTTACAGTTTCTGGAATGGGACGAATGTCCTTTAGACTCTTTCCCGTCAGACTTTCAACCAGATAACCTTGTTGAACTTCAGATGCATTCAAGCTGCCTCAAACAATTATGGAGAGGAAAAAAAGGATGGAGAATGCTACGGCTTATTGATATGAGTAGTTCAGAATACTTGATGTCGACTCCAGATTTCACTGAGGTTCCATTTCTTGAGATATTGGTGCTTCAAGATTGTACGAGGTTGGTTGAGGTTCACCCTTCTCTTGGAGTTCTCAAGAAACTTGTCTCTTTAAATATGAGAAATTGCAAGTCAGTTGAGAGCATTCCACCTTTCATTAGTTTGGAGTCCCTTAAAACTTTGAATCTTTCACTCTGTTCAAAACTCAAGAAGTTTCCTGAAATTGAAGGAAATATGAAAAACTTGCTGGAGCTTCATTTAGATGGGACTGGTATTGAGGAATTGCCTCTATCAGTTGAAGGTTTGACTGGTCTGACCACATTAAATTTAACAGATTGTAACAACCTTTTGCGTCTCCCCAGTACCATTCAGTCCTTGACATCTTTGAAAAGTCTCATTCTAACAGGTTGTTCAAAATTTGATGAGATAACTGAGAATCTGAATCATGTGGAATGCCTGGAGAAGCTTAATATAGGCGGAACTGCAATAAGAGATTTATCTTTCCTTGTAGGTATGAAGAATTTGAATGGTCTATACTGCCGAGGATGTAAAGGTCTAGCTTTAGGATCGTGGAAAGGTCATTCTGAAAGTTTGTTGTTTCCTGCTCTATTATCCAGTTTGACTTCTTTGGTAGAGCTAGATTTAAGGGATTGCAATCTGGTGGATGGAACCATTCCCAATGATCTTAGCAGCTTAATCTCTTTAAGAGAATTAGGTTTAGGTGGTAATAGTTTTGTTCGATTACCTGAAAGTATCTCTCAACTCTCAAAGCTCGAGAAATTAGAGTTGAGCAATTGTAAACAGCTTCAGTTGGTGCCCAAGCTTCCATCAAGTGCTCGACTGGTGCTTGCAGAAGATTGTACTTCACTGATAGATTTCCCAAATCAAATCAAATTATTGCCTTCAATTGAGTCTGGGATGACTACTATCAATTCACTCAGTTCTGCAAGTAGTTCAGCACTTCGAGACTCACATGGTTTCAGTACGTGGAAATCTTCAGCAAATGCCCAACCTTCTGAGGTGCTTTTGACACATGAGCATAGAGAGTGGAAACGAGTTGATCAAGTGTCACTGCCACTTCCAATGCTTATGAAAGACATTGAACTTTCTGATCCATCATCCAAACCCAGTGATATAATTTATTTTGATAATGAAATTCCAGAGTGGTTCAGCACTATATCTACCACCAATCCTATATCAATCTCTATTCCTCCAGATCTGACAGATGATGACAAGTGGAAGGGGGTTACAATGTGTGCTGTTTTTGCAGTCAAGGGGCATAATGTTCTCTCTGTTATTGAATCGGATTTAGAACTTTCAAACTACTTCTATCAGTGCACAATGGAAACAGATGTTTTTCATATGAAACCATTCATCTTTGACGGGGAAGAAATCCGCCACCTTCGATCAAGTTCTCATCTGGTTTTGATTTCCTATGTAGCTAGATGGCAGTTTCCAAAGTGGGAGTTAAATCAATCAACCACGGTGGCGGCTAAATTTGAAACCAACAATCCATTCATGGAGGTCCAGAAATGTGGGATTCGTCTAGTTTATGAGCAAGATGCTGGATGGTATACCAAACTATTCCCTGGGGTTAATGCAATTTGTCTAAATGAAGTGATGGATGCTATGCTGAAAACTGGCTGGCTTAGTCAAGTGGATCAATATTTGAAATGGGAGAAAATTATTCCCCCCATCGAAGAAGACTCCAGTACAGTTCTGCTAAGGAAGAACATCGAGTCTGTTCTTCCTCGATACCTTGAGGCATTGAACCATTATCCTGCAACATACAAATTTAACCTCAGAGGAAGCCCGTCGTGGTTTGAGTCCTTTTTCACTGATACAAGGTCAGGGTCGACTCTAGTGTCAATTAAGCTGCCTCAAAATCTACACAAGAGTAAGAAGTGGATGGCATTTGCAATATGCGCATCAATTGCAGAGGAACAGCAAGAGGACTATCATGTTTATTTTAAACTCAAAACGGGTGAATACAATGAGGATCTGTCCCGAGAACTTGAGTACATTGTGGCAATGTCGTCACAAGCAAATCATTATCAACTTTTGGTTGTGTACATACCGCGGGCATTGATTCCCGAAGTCTTGCTCGCTTGGACATCAACAACTGAAATGCACATTTCCTTCAGAGTCGATACTCCTGGTGCAGAAGTTCAAAGAAGTTGCGGCCGTATTGTTTACCAGGAAGATTTAGAAGGGTTTGTTGAAACAATCGTCCGGTGCATGCAAAGAGAAGATGCTCTTGAACTTTATGATAAATGGGTGGTAGAAGATTGGATAGACTTGATAGGATTGCAAGGGGGTCATTTATGTACTCCATACAAATGCAATTCCATAACAGGACGGGAAAGGAAGTTTGAATTGCTCTCTCAGAAATATAGGAATCGGGACTGGGCTATGTCTCCTCCTTATATTTGCGTCTTCTTTGGAGCTGAAATTTCCAAGTGGAATTGGTTCATGCACTTCAATGAGGGAAACACTGCAAAAATCCAACTGCCTCCTAATTTGTTCTATGATGCTAATTGGATGGGGATTGCTGTATGTGGTTTATGTTGGGCGTCCCACGAAGATTCAAACATTATACTAGACGGTAGTCCTGACACAGAGTATTATGTACTCAATTTGTGTCTGGACTCTGATGTCGGTTCGGACGGCTTATTTGATGTCAAATTACAGAAACTTTTTGGGAAACTCAAGCCATGGGAATTTGAAACCGATATCACATTTTTCTTCTATATACCTCGTGTTAAATATTTAAAGTTTTGGAGACAATTCAACCTTGCCAGGGTTACTTTTTCATCTTCTAGCCCATGCTTAAGAGTCTATAGTTGTGCCCTTCGTCTAGTATTTAAGGAGGACGTTGAAGATCTCGTTCGGACATTGACACTCTCCGAAATACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1416

Amino Acids

161.26

Weight (kDa)

5.44

Isoelectric Point (pI)

47.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 5 - 171 6.1e-23 NB-ARC domain
NACHT PF05729 22 - 168 5.2e-06 NACHT domain
WHD_ROQ1 PF23282 240 - 308 5.7e-16 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 481 - 619 2.1e-10 Leucine-rich repeat region
LRR_13 PF23286 499 - 587 4e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 647 - 722 1e-06 Leucine-rich repeat region
C-JID PF20160 828 - 966 6.7e-09 C-JID domain
C-JID PF20160 1266 - 1404 8.4e-07 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 3196, 3986
Acc16I TGCGCA 1 cut(s) 3259
AccB1I GGYRCC 1 cut(s) 2146
AccI GTMKAC 4 cut(s) 624, 1334, 2906, 3188
AccII CGCG 1 cut(s) 3412
AccIII TCCGGA 2 cut(s) 49, 814
AciI CCGC 6 cut(s) 1809, 2728, 2822, 3410, 3412, 3513
AclWI GGATC 5 cut(s) 395, 1906, 2438, 3000, 3339
AcoI YGGCCR 1 cut(s) 3514
AcuI CTGAAG 8 cut(s) 464, 492, 557, 954, 1220, 2123, 2313, 3457
AcyI GRCGYC 1 cut(s) 3905
AfiI CCNNNNNNNGG 3 cut(s) 814, 1418, 4132
AflII CTTAAG 1 cut(s) 4162
AjnI CCWGG 8 cut(s) 376, 837, 940, 1788, 2920, 3487, 3528, 4130
AjuI GAANNNNNNNTTGG 4 cut(s) 719, 751, 2831, 2863
Alw21I GWGCWC 2 cut(s) 2170, 2672
Alw26I GTCTC 6 cut(s) 1444, 1691, 1730, 2292, 3753, 4108
Alw44I GTGCAC 1 cut(s) 2668
AlwI GGATC 5 cut(s) 395, 1906, 2438, 3000, 3339
AlwNI CAGNNNCTG 1 cut(s) 1168
Ama87I CYCGRG 2 cut(s) 2105, 3339
Aor13HI TCCGGA 2 cut(s) 49, 814
AoxI GGCC 2 cut(s) 571, 3514
ApaLI GTGCAC 1 cut(s) 2668
ApeKI GCWGC 4 cut(s) 150, 1251, 2022, 3208
ArsI GACNNNNNNTTYG 2 cut(s) 3989, 4021
AseI ATTAAT 1 cut(s) 681
Asp700I GAANNNNTTC 4 cut(s) 477, 531, 2702, 3800
AspLEI GCGC 1 cut(s) 3260
AspS9I GGNCC 3 cut(s) 328, 374, 2857
AsuHPI GGTGA 3 cut(s) 463, 1400, 3329
AsuII TTCGAA 1 cut(s) 221
AvaI CYCGRG 2 cut(s) 2105, 3339
AvaII GGWCC 3 cut(s) 328, 374, 2857
BaeGI GKGCMC 3 cut(s) 2151, 2672, 4183
BaeI ACNNNNGTAYC 2 cut(s) 884, 917
BanI GGYRCC 1 cut(s) 2146
BauI CACGAG 1 cut(s) 4089
BbsI GAAGAC 2 cut(s) 3042, 3760
Bbv12I GWGCWC 2 cut(s) 2170, 2672
BbvI GCAGC 4 cut(s) 162, 1238, 2034, 3195
BceAI ACGGC 3 cut(s) 1313, 3501, 4015
BciT130I CCWGG 8 cut(s) 378, 839, 942, 1790, 2922, 3489, 3530, 4132
BclI TGATCA 2 cut(s) 991, 2392
BcoDI GTCTC 6 cut(s) 1444, 1691, 1730, 2292, 3753, 4108
BfmI CTRYAG 2 cut(s) 1011, 4171
BfrI CTTAAG 1 cut(s) 4162
BglII AGATCT 2 cut(s) 2548, 4213
BisI GCNGC 6 cut(s) 151, 1252, 2023, 2823, 3209, 3514
BlsI GCNGC 6 cut(s) 152, 1253, 2024, 2824, 3210, 3515
Bme1390I CCNGG 8 cut(s) 378, 839, 942, 1790, 2922, 3489, 3530, 4132
Bme18I GGWCC 3 cut(s) 328, 374, 2857
BmeT110I CYCGRG 2 cut(s) 2105, 3339
BmgT120I GGNCC 3 cut(s) 328, 374, 2857
BmiI GGNNCC 3 cut(s) 1356, 2002, 2148
BmrFI CCNGG 8 cut(s) 378, 839, 942, 1790, 2922, 3489, 3530, 4132
BmrI ACTGGG 3 cut(s) 1686, 2454, 3748
BmsI GCATC 9 cut(s) 457, 793, 870, 1230, 2884, 2947, 3269, 3574, 3853
BmuI ACTGGG 3 cut(s) 1686, 2454, 3748
BoxI GACNNNNGTC 2 cut(s) 1182, 3939
BpiI GAAGAC 2 cut(s) 3042, 3760
BpmI CTGGAG 5 cut(s) 1323, 1601, 1811, 2529, 3025
Bpu10I CCTNAGC 1 cut(s) 3056
Bpu14I TTCGAA 1 cut(s) 221
BpuEI CTTGAG 7 cut(s) 179, 1385, 1412, 1523, 3113, 3368, 4025
BsaAI YACGTR 1 cut(s) 2319
BsaBI GATNNNNATC 1 cut(s) 1986
BsaHI GRCGYC 1 cut(s) 3905
BsaJI CCNNGG 8 cut(s) 377, 1873, 2814, 2920, 2921, 3410, 4046, 4131
BsaWI WCCGGW 3 cut(s) 49, 814, 3564
BsaXI ACNNNNNCTCC 4 cut(s) 426, 456, 819, 849
Bsc4I CCNNNNNNNGG 3 cut(s) 814, 1418, 4132
Bse1I ACTGG 9 cut(s) 1606, 1645, 1692, 1947, 2178, 2460, 2977, 3042, 3743
Bse3DI GCAATG 2 cut(s) 741, 3369
Bse8I GATNNNNATC 1 cut(s) 1986
BseAI TCCGGA 2 cut(s) 49, 814
BseBI CCWGG 8 cut(s) 378, 839, 942, 1790, 2922, 3489, 3530, 4132
BseDI CCNNGG 8 cut(s) 377, 1873, 2814, 2920, 2921, 3410, 4046, 4131
BseJI GATNNNNATC 1 cut(s) 1986
BseLI CCNNNNNNNGG 3 cut(s) 814, 1418, 4132
BseMI GCAATG 2 cut(s) 741, 3369
BseNI ACTGG 9 cut(s) 1606, 1645, 1692, 1947, 2178, 2460, 2977, 3042, 3743
BseRI GAGGAG 2 cut(s) 1019, 3741
BseSI GKGCMC 3 cut(s) 2151, 2672, 4183
BseX3I CGGCCG 1 cut(s) 3514
BseXI GCAGC 4 cut(s) 162, 1238, 2034, 3195
BsgI GTGCAG 3 cut(s) 213, 444, 3513
Bsh1236I CGCG 1 cut(s) 3412
Bsh1285I CGRYCG 1 cut(s) 3517
BshFI GGCC 2 cut(s) 573, 3516
BshNI GGYRCC 1 cut(s) 2146
BsiEI CGRYCG 1 cut(s) 3517
BsiHKAI GWGCWC 2 cut(s) 2170, 2672
BsiHKCI CYCGRG 2 cut(s) 2105, 3339
BsiSI CCGG 3 cut(s) 50, 815, 3565
BslFI GGGAC 6 cut(s) 1190, 1486, 1612, 3322, 3749, 3892
BslI CCNNNNNNNGG 3 cut(s) 814, 1418, 4132
BsmAI GTCTC 6 cut(s) 1444, 1691, 1730, 2292, 3753, 4108
BsmBI CGTCTC 1 cut(s) 1691
BsmFI GGGAC 6 cut(s) 1190, 1486, 1612, 3322, 3749, 3892
BsmI GAATGC 4 cut(s) 1243, 1296, 1476, 1790
BsnI GGCC 2 cut(s) 573, 3516
BsoBI CYCGRG 2 cut(s) 2105, 3339
Bsp119I TTCGAA 1 cut(s) 221
Bsp1286I GDGCHC 4 cut(s) 2151, 2170, 2672, 4183
Bsp13I TCCGGA 2 cut(s) 49, 814
Bsp1407I TGTACA 3 cut(s) 247, 826, 3402
Bsp19I CCATGG 1 cut(s) 4046
BspACI CCGC 6 cut(s) 1809, 2728, 2822, 3410, 3412, 3513
BspANI GGCC 2 cut(s) 573, 3516
BspEI TCCGGA 2 cut(s) 49, 814
BspFNI CGCG 1 cut(s) 3412
BspLI GGNNCC 3 cut(s) 1356, 2002, 2148
BspMAI CTGCAG 1 cut(s) 1015
BspPI GGATC 5 cut(s) 395, 1906, 2438, 3000, 3339
BspQI GCTCTTC 2 cut(s) 483, 930
BspT104I TTCGAA 1 cut(s) 221
BspT107I GGYRCC 1 cut(s) 2146
BspTI CTTAAG 1 cut(s) 4162
BsrDI GCAATG 2 cut(s) 741, 3369
BsrGI TGTACA 3 cut(s) 247, 826, 3402
BsrI ACTGG 9 cut(s) 1606, 1645, 1692, 1947, 2178, 2460, 2977, 3042, 3743
BssECI CCNNGG 8 cut(s) 377, 1873, 2814, 2920, 2921, 3410, 4046, 4131
BssNAI GTATAC 1 cut(s) 2907
BssNI GRCGYC 1 cut(s) 3905
BssSI CACGAG 1 cut(s) 4089
BssT1I CCWWGG 1 cut(s) 4046
Bst1107I GTATAC 1 cut(s) 2907
Bst2BI CACGAG 1 cut(s) 4089
Bst2UI CCWGG 8 cut(s) 378, 839, 942, 1790, 2922, 3489, 3530, 4132
Bst4CI ACNGT 7 cut(s) 812, 859, 900, 1164, 2818, 3049, 3938
Bst6I CTCTTC 2 cut(s) 483, 930
BstACI GRCGYC 1 cut(s) 3905
BstAFI CTTAAG 1 cut(s) 4162
BstAPI GCANNNNNTGC 2 cut(s) 1010, 3266
BstAUI TGTACA 3 cut(s) 247, 826, 3402
BstBAI YACGTR 1 cut(s) 2319
BstBI TTCGAA 1 cut(s) 221
BstC8I GCNNGC 5 cut(s) 2181, 2978, 3414, 3438, 3572
BstDSI CCRYGG 3 cut(s) 2814, 3410, 4046
BstFNI CGCG 1 cut(s) 3412
BstHHI GCGC 1 cut(s) 3260
BstMAI GTCTC 6 cut(s) 1444, 1691, 1730, 2292, 3753, 4108
BstMCI CGRYCG 1 cut(s) 3517
BstMWI GCNNNNNNNGC 4 cut(s) 1010, 2597, 3257, 3266
BstNI CCWGG 8 cut(s) 378, 839, 942, 1790, 2922, 3489, 3530, 4132
BstNSI RCATGY 2 cut(s) 750, 3574
BstPAI GACNNNNGTC 2 cut(s) 1182, 3939
BstSCI CCNGG 8 cut(s) 376, 837, 940, 1788, 2920, 3487, 3528, 4130
BstSFI CTRYAG 2 cut(s) 1011, 4171
BstSLI GKGCMC 3 cut(s) 2151, 2672, 4183
BstUI CGCG 1 cut(s) 3412
BstV1I GCAGC 4 cut(s) 162, 1238, 2034, 3195
BstV2I GAAGAC 2 cut(s) 3042, 3760
BstX2I RGATCY 3 cut(s) 2548, 3331, 4213
BstXI CCANNNNNNTGG 2 cut(s) 521, 2867
BstYI RGATCY 3 cut(s) 2548, 3331, 4213
BstZ17I GTATAC 1 cut(s) 2907
BstZI CGGCCG 1 cut(s) 3514
BsuRI GGCC 2 cut(s) 573, 3516
BtgI CCRYGG 3 cut(s) 2814, 3410, 4046
BtsI GCAGTG 4 cut(s) 201, 1008, 2402, 3825
Cac8I GCNNGC 5 cut(s) 2181, 2978, 3414, 3438, 3572
CaiI CAGNNNCTG 1 cut(s) 1168
CfoI GCGC 1 cut(s) 3260
Cfr13I GGNCC 3 cut(s) 328, 374, 2857
Cfr42I CCGCGG 1 cut(s) 3413
CseI GACGC 3 cut(s) 1673, 3754, 3894
CspCI CAANNNNNGTGG 2 cut(s) 2799, 2834
DraI TTTAAA 4 cut(s) 757, 1446, 3304, 4104
DrdI GACNNNNNNGTC 2 cut(s) 3196, 3986
DseDI GACNNNNNNGTC 2 cut(s) 3196, 3986
EaeI YGGCCR 1 cut(s) 3514
EagI CGGCCG 1 cut(s) 3514
Eam1104I CTCTTC 2 cut(s) 483, 930
EarI CTCTTC 2 cut(s) 483, 930
EciI GGCGGA 2 cut(s) 1824, 2717
EclXI CGGCCG 1 cut(s) 3514
Eco130I CCWWGG 1 cut(s) 4046
Eco32I GATATC 1 cut(s) 4066
Eco47I GGWCC 3 cut(s) 328, 374, 2857
Eco52I CGGCCG 1 cut(s) 3514
Eco57I CTGAAG 8 cut(s) 464, 492, 557, 954, 1220, 2123, 2313, 3457
Eco88I CYCGRG 2 cut(s) 2105, 3339
EcoRII CCWGG 8 cut(s) 376, 837, 940, 1788, 2920, 3487, 3528, 4130
EcoRV GATATC 1 cut(s) 4066
EcoT14I CCWWGG 1 cut(s) 4046
EcoT22I ATGCAT 2 cut(s) 885, 1245
ErhI CCWWGG 1 cut(s) 4046
Esp3I CGTCTC 1 cut(s) 1691
FalI AAGNNNNNCTT 4 cut(s) 198, 230, 3573, 3605
FaqI GGGAC 6 cut(s) 1190, 1486, 1612, 3322, 3749, 3892
FauI CCCGC 1 cut(s) 3405
FauNDI CATATG 1 cut(s) 2694
FbaI TGATCA 2 cut(s) 991, 2392
FblI GTMKAC 4 cut(s) 624, 1334, 2906, 3188
Fnu4HI GCNGC 6 cut(s) 151, 1252, 2023, 2823, 3209, 3514
Fsp4HI GCNGC 6 cut(s) 151, 1252, 2023, 2823, 3209, 3514
FspAI RTGCGCAY 1 cut(s) 3259
FspI TGCGCA 1 cut(s) 3259
GlaI GCGC 1 cut(s) 3259
GluI GCNGC 6 cut(s) 151, 1252, 2023, 2823, 3209, 3514
GsuI CTGGAG 5 cut(s) 1323, 1601, 1811, 2529, 3025
HaeIII GGCC 2 cut(s) 573, 3516
HapII CCGG 3 cut(s) 50, 815, 3565
HgaI GACGC 3 cut(s) 1673, 3754, 3894
HhaI GCGC 1 cut(s) 3260
Hin1I GRCGYC 1 cut(s) 3905
Hin6I GCGC 1 cut(s) 3258
HinP1I GCGC 1 cut(s) 3258
HincII GTYRAC 2 cut(s) 1335, 3189
HindII GTYRAC 2 cut(s) 1335, 3189
HindIII AAGCTT 2 cut(s) 1796, 2153
HpaII CCGG 3 cut(s) 50, 815, 3565
HphI GGTGA 3 cut(s) 463, 1400, 3329
Hpy99I CGWCG 2 cut(s) 1041, 3151
HpyCH4III ACNGT 7 cut(s) 812, 859, 900, 1164, 2818, 3049, 3938
HpyCH4IV ACGT 5 cut(s) 949, 1036, 1119, 2318, 4206
HpyF10VI GCNNNNNNNGC 4 cut(s) 1010, 2597, 3257, 3266
HpySE526I ACGT 5 cut(s) 949, 1036, 1119, 2318, 4206
Hsp92I GRCGYC 1 cut(s) 3905
HspAI GCGC 1 cut(s) 3258
Kpn2I TCCGGA 2 cut(s) 49, 814
Ksp22I TGATCA 2 cut(s) 991, 2392
KspI CCGCGG 1 cut(s) 3413
LguI GCTCTTC 2 cut(s) 483, 930
LmnI GCTCC 2 cut(s) 1582, 3776
Lsp1109I GCAGC 4 cut(s) 162, 1238, 2034, 3195
LweI GCATC 9 cut(s) 457, 793, 870, 1230, 2884, 2947, 3269, 3574, 3853
MaeII ACGT 5 cut(s) 949, 1036, 1119, 2318, 4206
MaeIII GTNAC 6 cut(s) 1158, 1670, 2400, 2578, 3369, 4135
MfeI CAATTG 4 cut(s) 341, 2125, 2238, 3264
MflI RGATCY 3 cut(s) 2548, 3331, 4213
MhlI GDGCHC 4 cut(s) 2151, 2170, 2672, 4183
MmeI TCCRAC 3 cut(s) 502, 1057, 3862
Mph1103I ATGCAT 2 cut(s) 885, 1245
MroI TCCGGA 2 cut(s) 49, 814
MroXI GAANNNNTTC 4 cut(s) 477, 531, 2702, 3800
MslI CAYNNNNRTG 6 cut(s) 15, 260, 1085, 1593, 3670, 3813
MspA1I CMGCKG 2 cut(s) 517, 3412
MspCI CTTAAG 1 cut(s) 4162
MspI CCGG 3 cut(s) 50, 815, 3565
MspR9I CCNGG 8 cut(s) 378, 839, 942, 1790, 2922, 3489, 3530, 4132
MunI CAATTG 4 cut(s) 341, 2125, 2238, 3264
Mva1269I GAATGC 4 cut(s) 1243, 1296, 1476, 1790
MvaI CCWGG 8 cut(s) 378, 839, 942, 1790, 2922, 3489, 3530, 4132
MvnI CGCG 1 cut(s) 3412
MwoI GCNNNNNNNGC 4 cut(s) 1010, 2597, 3257, 3266
NcoI CCATGG 1 cut(s) 4046
NdeI CATATG 1 cut(s) 2694
NlaIV GGNNCC 3 cut(s) 1356, 2002, 2148
NmeAIII GCCGAG 1 cut(s) 1898
NmuCI GTSAC 2 cut(s) 2400, 3369
NsbI TGCGCA 1 cut(s) 3259
NsiI ATGCAT 2 cut(s) 885, 1245
NspI RCATGY 2 cut(s) 750, 3574
NspV TTCGAA 1 cut(s) 221
PaeI GCATGC 1 cut(s) 3574
PaeR7I CTCGAG 1 cut(s) 2105
PasI CCCWGGG 1 cut(s) 2921
PciSI GCTCTTC 2 cut(s) 483, 930
PctI GAATGC 4 cut(s) 1243, 1296, 1476, 1790
PdmI GAANNNNTTC 4 cut(s) 477, 531, 2702, 3800
PfoI TCCNGGA 1 cut(s) 837
PkrI GCNGC 6 cut(s) 152, 1253, 2024, 2824, 3210, 3515
Ppu21I YACGTR 1 cut(s) 2319
PshAI GACNNNNGTC 2 cut(s) 1182, 3939
PshBI ATTAAT 1 cut(s) 681
Psp6I CCWGG 8 cut(s) 376, 837, 940, 1788, 2920, 3487, 3528, 4130
PspGI CCWGG 8 cut(s) 376, 837, 940, 1788, 2920, 3487, 3528, 4130
PspN4I GGNNCC 3 cut(s) 1356, 2002, 2148
PspPI GGNCC 3 cut(s) 328, 374, 2857
PstI CTGCAG 1 cut(s) 1015
PstNI CAGNNNCTG 1 cut(s) 1168
PsuI RGATCY 3 cut(s) 2548, 3331, 4213
PvuII CAGCTG 1 cut(s) 517
RseI CAYNNNNRTG 6 cut(s) 15, 260, 1085, 1593, 3670, 3813
SacII CCGCGG 1 cut(s) 3413
SalI GTCGAC 2 cut(s) 1333, 3187
SapI GCTCTTC 2 cut(s) 483, 930
SatI GCNGC 6 cut(s) 151, 1252, 2023, 2823, 3209, 3514
Sau96I GGNCC 3 cut(s) 328, 374, 2857
ScrFI CCNGG 8 cut(s) 378, 839, 942, 1790, 2922, 3489, 3530, 4132
SduI GDGCHC 4 cut(s) 2151, 2170, 2672, 4183
SfaNI GCATC 9 cut(s) 457, 793, 870, 1230, 2884, 2947, 3269, 3574, 3853
SfcI CTRYAG 2 cut(s) 1011, 4171
Sfr274I CTCGAG 1 cut(s) 2105
Sfr303I CCGCGG 1 cut(s) 3413
SfuI TTCGAA 1 cut(s) 221
SgrBI CCGCGG 1 cut(s) 3413
SinI GGWCC 3 cut(s) 328, 374, 2857
SlaI CTCGAG 1 cut(s) 2105
SmiMI CAYNNNNRTG 6 cut(s) 15, 260, 1085, 1593, 3670, 3813
SmlI CTYRAG 9 cut(s) 158, 1364, 1427, 1538, 2105, 3092, 3347, 4040, 4162
SmoI CTYRAG 9 cut(s) 158, 1364, 1427, 1538, 2105, 3092, 3347, 4040, 4162
SphI GCATGC 1 cut(s) 3574
SsiI CCGC 6 cut(s) 1809, 2728, 2822, 3410, 3412, 3513
SspI AATATT 3 cut(s) 789, 2999, 4100
StyD4I CCNGG 8 cut(s) 376, 837, 940, 1788, 2920, 3487, 3528, 4130
StyI CCWWGG 1 cut(s) 4046
TaaI ACNGT 7 cut(s) 812, 859, 900, 1164, 2818, 3049, 3938
TaiI ACGT 5 cut(s) 952, 1039, 1122, 2321, 4209
TatI WGTACW 9 cut(s) 247, 293, 826, 2192, 3044, 3351, 3402, 3659, 3961
TauI GCSGC 2 cut(s) 2825, 3516
TseFI GTSAC 2 cut(s) 2400, 3369
TseI GCWGC 4 cut(s) 150, 1251, 2022, 3208
Tsp45I GTSAC 2 cut(s) 2400, 3369
TspGWI ACGGA 1 cut(s) 908
Vha464I CTTAAG 1 cut(s) 4162
VneI GTGCAC 1 cut(s) 2668
VpaK11BI GGWCC 3 cut(s) 328, 374, 2857
VspI ATTAAT 1 cut(s) 681
XbaI TCTAGA 1 cut(s) 401
XceI RCATGY 2 cut(s) 750, 3574
XhoI CTCGAG 1 cut(s) 2105
XmiI GTMKAC 4 cut(s) 624, 1334, 2906, 3188
XmnI GAANNNNTTC 4 cut(s) 477, 531, 2702, 3800
Zsp2I ATGCAT 2 cut(s) 885, 1245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.