Rroxscaffold_2G00121850

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000129
Physical Location & Seq
Forward (+)
54655175 .. 54664948
9774 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00121850.1

Sequence Viewer

Length: 1329 bp
ATGGATTCTCACCTGAATGTAATGCTTTCGTACTTAGACATTGGGTGTCCCAATGTTCGTATCATAGGGATTTGTGGCATGGGTGGTATCGGTAAGACAACAATTGCACAAGTAGTTTTTGAAAGGTTACAAGCTCATTTTGAAGGTTGCAGCTTTCTTGAGAATGTTAGAGAGGTAACCGAAAAGCAAGGTGCAGTTCAATTACAAGAGAAACTTCTTTTGAATTTGCTAAAATGTAGTGTAAATGTACAGAACACTAAAATGGGAAAAAATATAATGAGGCAGAGACTGCGTACTAAAATGGTTCTAATCATTCTTGATGATGTGGACCAAGAAGAACAATTGGAAGCATTGTGTGATAGGGAATCGTTTGGTCCAGGGAGTAGAATCATCATAACTTCTAGAGATGAACATTTGCTCAGTGCAGTTGAAGGAGATAAAATATATAAGGTGAATCCACTAACTGATGCTGAAGCTCTTCAGCTCTTCAGTATGAAAGCCTTCAAGAAAGATCAGCAGGTTGGAGAAGAAATTTTGAAGCTATCCAAAGAATTTTTGAGATATGCTAATGGCCTTCCACTAGCTATTAAAGTTTTGGGTTCTTCTGTTAAGGGTAGAAGTGTAGGGTTATGGTTAAGTGCATTGGATGGACTTAAAGAAAATCCTGAAAAAAAAATCATTAATGTTCTCAAAGTCAGTTTTAATGGATTAAAACCAAGACAAAAGAAGATTTTTTTGGACATTGCATGTTTCTTTAAAGGGGAGAGCATAGATCGTGTAAGAAGAATATTACAAGGTAGTGATGCCCATTGTCCGGACTTTGATGTACAAGTTCTCCTGGACAAATCTCTGGTAACTATGTTTGGAAGAGAATTGGGGATGCATGACTTGGTACAAGAATTGGGTTGGGAAATTGTTCGTCAAGAATGTCGTGAAGAGCCTGGAAAACGTAGCAGGTTGTGGCTTGCCAATGAAATCATCCATGTACTTGGTTGGAATAGGGCGACGAGTGCAGTTCAAAGCATATTCCTCGAAGGTCCAATAGAAGAGGATGTTGTTCACTCAATTGATAATGCCTTCTCAAATATGGACAGATTGAGATTGCTCAAGATTAGGAATGTGAAGTTTTCCGGAAACATCAGATATCTTTCTAATGAGTTACAATATCTTGAATGGCATGAGTGTCCTTTGGATTCTTTCCCGTCAGACTTTCAGCCGGAGAAACTTGTTGAAGTTCACATGCATTTTAGTTGCATCAAACAACTATGGAGGGGGAAAAAAGTAAGATCACAATTAATTTTATGCATGTTCATAAAGCGGATAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

442

Amino Acids

50.51

Weight (kDa)

8.39

Isoelectric Point (pI)

45.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 18 - 172 1.5e-24 NB-ARC domain
NACHT PF05729 22 - 168 3.4e-06 NACHT domain
WHD_ROQ1 PF23282 240 - 308 3.9e-16 Disease resistance protein Roq1-like, winged-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 508, 945
AccIII TCCGGA 2 cut(s) 814, 1130
AciI CCGC 1 cut(s) 1318
AcsI RAATTY 3 cut(s) 223, 531, 551
AcuI CTGAAG 3 cut(s) 464, 472, 492
AfaI GTAC 6 cut(s) 32, 249, 295, 828, 894, 987
AfiI CCNNNNNNNGG 1 cut(s) 814
AjnI CCWGG 3 cut(s) 376, 837, 940
AjuI GAANNNNNNNTTGG 2 cut(s) 719, 751
AluBI AGCT 6 cut(s) 134, 153, 476, 484, 541, 584
AluI AGCT 6 cut(s) 134, 153, 476, 484, 541, 584
Alw26I GTCTC 1 cut(s) 280
AlwNI CAGNNNCTG 1 cut(s) 289
Aor13HI TCCGGA 2 cut(s) 814, 1130
AoxI GGCC 1 cut(s) 571
ApeKI GCWGC 1 cut(s) 150
ApoI RAATTY 3 cut(s) 223, 531, 551
AseI ATTAAT 2 cut(s) 681, 1295
Asp700I GAANNNNTTC 3 cut(s) 477, 500, 915
AspS9I GGNCC 3 cut(s) 328, 374, 1037
AsuHPI GGTGA 1 cut(s) 463
AvaII GGWCC 3 cut(s) 328, 374, 1037
BbvI GCAGC 1 cut(s) 162
BccI CCATC 1 cut(s) 641
BcgI CGANNNNNNTGC 2 cut(s) 1012, 1046
BciT130I CCWGG 3 cut(s) 378, 839, 942
BcoDI GTCTC 1 cut(s) 280
BfaI CTAG 2 cut(s) 402, 581
BfuAI ACCTGC 2 cut(s) 508, 945
BisI GCNGC 1 cut(s) 151
BlsI GCNGC 1 cut(s) 152
Bme1390I CCNGG 3 cut(s) 378, 839, 942
Bme18I GGWCC 3 cut(s) 328, 374, 1037
BmgT120I GGNCC 3 cut(s) 328, 374, 1037
BmrFI CCNGG 3 cut(s) 378, 839, 942
BmsI GCATC 4 cut(s) 457, 793, 870, 1263
BpuEI CTTGAG 2 cut(s) 179, 1091
BsaJI CCNNGG 1 cut(s) 377
BsaWI WCCGGW 2 cut(s) 814, 1130
BsaXI ACNNNNNCTCC 2 cut(s) 819, 849
Bsc4I CCNNNNNNNGG 1 cut(s) 814
Bse3DI GCAATG 1 cut(s) 741
BseAI TCCGGA 2 cut(s) 814, 1130
BseBI CCWGG 3 cut(s) 378, 839, 942
BseDI CCNNGG 1 cut(s) 377
BseGI GGATG 4 cut(s) 652, 885, 978, 1057
BseLI CCNNNNNNNGG 1 cut(s) 814
BseMI GCAATG 1 cut(s) 741
BseMII CTCAG 1 cut(s) 433
BseXI GCAGC 1 cut(s) 162
BsgI GTGCAG 3 cut(s) 213, 444, 1032
BshFI GGCC 1 cut(s) 573
BsiSI CCGG 3 cut(s) 815, 1131, 1217
BslFI GGGAC 1 cut(s) 33
BslI CCNNNNNNNGG 1 cut(s) 814
BsmAI GTCTC 1 cut(s) 280
BsmFI GGGAC 1 cut(s) 33
BsnI GGCC 1 cut(s) 573
Bsp13I TCCGGA 2 cut(s) 814, 1130
Bsp1407I TGTACA 2 cut(s) 247, 826
Bsp143I GATC 3 cut(s) 511, 772, 1286
BspACI CCGC 1 cut(s) 1318
BspANI GGCC 1 cut(s) 573
BspCNI CTCAG 1 cut(s) 432
BspEI TCCGGA 2 cut(s) 814, 1130
BspMI ACCTGC 2 cut(s) 508, 945
BspQI GCTCTTC 3 cut(s) 483, 491, 930
BsrDI GCAATG 1 cut(s) 741
BsrGI TGTACA 2 cut(s) 247, 826
BssECI CCNNGG 1 cut(s) 377
BssMI GATC 3 cut(s) 511, 772, 1286
Bst2UI CCWGG 3 cut(s) 378, 839, 942
Bst6I CTCTTC 5 cut(s) 483, 491, 862, 930, 1041
BstAPI GCANNNNNTGC 1 cut(s) 289
BstAUI TGTACA 2 cut(s) 247, 826
BstC8I GCNNGC 1 cut(s) 966
BstDEI CTNAG 2 cut(s) 34, 419
BstEII GGTNACC 1 cut(s) 175
BstF5I GGATG 4 cut(s) 652, 885, 978, 1057
BstKTI GATC 3 cut(s) 514, 775, 1289
BstMAI GTCTC 1 cut(s) 280
BstMBI GATC 3 cut(s) 511, 772, 1286
BstMWI GCNNNNNNNGC 2 cut(s) 289, 1010
BstNI CCWGG 3 cut(s) 378, 839, 942
BstNSI RCATGY 3 cut(s) 750, 1243, 1309
BstPI GGTNACC 1 cut(s) 175
BstSCI CCNGG 3 cut(s) 376, 837, 940
BstV1I GCAGC 1 cut(s) 162
BstXI CCANNNNNNTGG 1 cut(s) 989
BsuRI GGCC 1 cut(s) 573
BtsCI GGATG 4 cut(s) 652, 885, 978, 1057
BtsIMutI CAGTG 1 cut(s) 427
BveI ACCTGC 2 cut(s) 508, 945
Cac8I GCNNGC 1 cut(s) 966
CaiI CAGNNNCTG 1 cut(s) 289
Cfr13I GGNCC 3 cut(s) 328, 374, 1037
Csp6I GTAC 6 cut(s) 31, 248, 294, 827, 893, 986
CviAII CATG 7 cut(s) 79, 747, 884, 983, 1178, 1240, 1306
CviQI GTAC 6 cut(s) 31, 248, 294, 827, 893, 986
DdeI CTNAG 2 cut(s) 34, 419
DpnI GATC 3 cut(s) 513, 774, 1288
DpnII GATC 3 cut(s) 511, 772, 1286
DraI TTTAAA 1 cut(s) 757
Eam1104I CTCTTC 5 cut(s) 483, 491, 862, 930, 1041
EarI CTCTTC 5 cut(s) 483, 491, 862, 930, 1041
Eco32I GATATC 1 cut(s) 1145
Eco47I GGWCC 3 cut(s) 328, 374, 1037
Eco57I CTGAAG 3 cut(s) 464, 472, 492
Eco91I GGTNACC 1 cut(s) 175
EcoO65I GGTNACC 1 cut(s) 175
EcoRII CCWGG 3 cut(s) 376, 837, 940
EcoRV GATATC 1 cut(s) 1145
EcoT22I ATGCAT 3 cut(s) 885, 1245, 1307
FaeI CATG 7 cut(s) 82, 750, 887, 986, 1181, 1243, 1309
FalI AAGNNNNNCTT 2 cut(s) 198, 230
FaqI GGGAC 1 cut(s) 33
FatI CATG 7 cut(s) 78, 746, 883, 982, 1177, 1239, 1305
Fnu4HI GCNGC 1 cut(s) 151
FokI GGATG 4 cut(s) 659, 892, 965, 1064
Fsp4HI GCNGC 1 cut(s) 151
FspBI CTAG 2 cut(s) 402, 581
GluI GCNGC 1 cut(s) 151
HaeIII GGCC 1 cut(s) 573
HapII CCGG 3 cut(s) 815, 1131, 1217
Hin1II CATG 7 cut(s) 82, 750, 887, 986, 1181, 1243, 1309
HinfI GANTC 5 cut(s) 5, 365, 387, 454, 1193
HpaII CCGG 3 cut(s) 815, 1131, 1217
HphI GGTGA 1 cut(s) 463
Hpy166II GTNNAC 3 cut(s) 328, 1060, 1237
Hpy188I TCNGA 2 cut(s) 1142, 1207
Hpy8I GTNNAC 3 cut(s) 328, 1060, 1237
Hpy99I CGWCG 1 cut(s) 1009
HpyAV CCTTC 6 cut(s) 137, 425, 511, 584, 1028, 1087
HpyCH4IV ACGT 1 cut(s) 949
HpyF10VI GCNNNNNNNGC 2 cut(s) 289, 1010
HpyF3I CTNAG 2 cut(s) 34, 419
HpySE526I ACGT 1 cut(s) 949
Hsp92II CATG 7 cut(s) 82, 750, 887, 986, 1181, 1243, 1309
Kpn2I TCCGGA 2 cut(s) 814, 1130
Kzo9I GATC 3 cut(s) 511, 772, 1286
LguI GCTCTTC 3 cut(s) 483, 491, 930
Lsp1109I GCAGC 1 cut(s) 162
LweI GCATC 4 cut(s) 457, 793, 870, 1263
MaeI CTAG 2 cut(s) 402, 581
MaeII ACGT 1 cut(s) 949
MaeIII GTNAC 4 cut(s) 126, 175, 853, 1158
MalI GATC 3 cut(s) 513, 774, 1288
MboI GATC 3 cut(s) 511, 772, 1286
MfeI CAATTG 3 cut(s) 102, 341, 1065
MmeI TCCRAC 2 cut(s) 502, 974
MnlI CCTC 5 cut(s) 166, 273, 1040, 1042, 1263
Mph1103I ATGCAT 3 cut(s) 885, 1245, 1307
MroI TCCGGA 2 cut(s) 814, 1130
MroXI GAANNNNTTC 3 cut(s) 477, 500, 915
MseI TTAA 9 cut(s) 588, 609, 635, 654, 681, 702, 710, 756, 1295
MslI CAYNNNNRTG 2 cut(s) 15, 260
MspI CCGG 3 cut(s) 815, 1131, 1217
MspR9I CCNGG 3 cut(s) 378, 839, 942
MunI CAATTG 3 cut(s) 102, 341, 1065
MvaI CCWGG 3 cut(s) 378, 839, 942
MwoI GCNNNNNNNGC 2 cut(s) 289, 1010
NdeII GATC 3 cut(s) 511, 772, 1286
NlaIII CATG 7 cut(s) 82, 750, 887, 986, 1181, 1243, 1309
NsiI ATGCAT 3 cut(s) 885, 1245, 1307
NspI RCATGY 3 cut(s) 750, 1243, 1309
PciSI GCTCTTC 3 cut(s) 483, 491, 930
PdmI GAANNNNTTC 3 cut(s) 477, 500, 915
PfeI GAWTC 5 cut(s) 5, 365, 387, 454, 1193
PfoI TCCNGGA 1 cut(s) 837
PkrI GCNGC 1 cut(s) 152
PshBI ATTAAT 2 cut(s) 681, 1295
Psp6I CCWGG 3 cut(s) 376, 837, 940
PspEI GGTNACC 1 cut(s) 175
PspGI CCWGG 3 cut(s) 376, 837, 940
PspPI GGNCC 3 cut(s) 328, 374, 1037
PstNI CAGNNNCTG 1 cut(s) 289
RsaI GTAC 6 cut(s) 32, 249, 295, 828, 894, 987
RsaNI GTAC 6 cut(s) 31, 248, 294, 827, 893, 986
RseI CAYNNNNRTG 2 cut(s) 15, 260
SapI GCTCTTC 3 cut(s) 483, 491, 930
SaqAI TTAA 9 cut(s) 588, 609, 635, 654, 681, 702, 710, 756, 1295
SatI GCNGC 1 cut(s) 151
Sau3AI GATC 3 cut(s) 511, 772, 1286
Sau96I GGNCC 3 cut(s) 328, 374, 1037
ScrFI CCNGG 3 cut(s) 378, 839, 942
SfaNI GCATC 4 cut(s) 457, 793, 870, 1263
SinI GGWCC 3 cut(s) 328, 374, 1037
SmiMI CAYNNNNRTG 2 cut(s) 15, 260
SmlI CTYRAG 2 cut(s) 158, 1106
SmoI CTYRAG 2 cut(s) 158, 1106
SsiI CCGC 1 cut(s) 1318
SspI AATATT 1 cut(s) 789
SspMI CTAG 2 cut(s) 402, 581
StyD4I CCNGG 3 cut(s) 376, 837, 940
TaiI ACGT 1 cut(s) 952
TaqI TCGA 1 cut(s) 1032
TatI WGTACW 3 cut(s) 247, 826, 985
TfiI GAWTC 5 cut(s) 5, 365, 387, 454, 1193
Tru1I TTAA 9 cut(s) 588, 609, 635, 654, 681, 702, 710, 756, 1295
Tru9I TTAA 9 cut(s) 588, 609, 635, 654, 681, 702, 710, 756, 1295
TscAI CASTG 1 cut(s) 427
TseI GCWGC 1 cut(s) 150
TspDTI ATGAA 4 cut(s) 423, 509, 987, 1300
TspRI CASTG 1 cut(s) 427
VpaK11BI GGWCC 3 cut(s) 328, 374, 1037
VspI ATTAAT 2 cut(s) 681, 1295
XapI RAATTY 3 cut(s) 223, 531, 551
XbaI TCTAGA 1 cut(s) 401
XceI RCATGY 3 cut(s) 750, 1243, 1309
XmnI GAANNNNTTC 3 cut(s) 477, 500, 915
XspI CTAG 2 cut(s) 402, 581
Zsp2I ATGCAT 3 cut(s) 885, 1245, 1307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.