FvH4_5g38520

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000129
Physical Location & Seq
Reverse (-)
28471955 .. 28473263
1309 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g38520.t1

Sequence Viewer

Length: 1062 bp
ATGGATTCCCACCTGAATGAAATGCTTTCATACTTAGACTTTGGTTGTCCTGATGTTCGGATCATAGGCATTTGGGGTATGGGTGGTGTCGGAAAAACAACTATTGCACAAGTGGTTTCTGAGAGGGTAAAAGCTCAGTTTGAAGGTGATAGCTTTCTTGAGAATGTTAGAGAGGTAACTGAAAAGCAAGGTGTGGTTCATTTACAGCAGCAACTTCTTTCAAACTTGCTGAAAAGTACTGAACATGTACAAACCAGTAAAATGGGAAAAGATATAATCAGGCATAGACTATGTGATAGAAAGGTTCTTCTCATTCTTGATGATGTGGATCAGGACGAACAATTGCAAGCATTGTGTGGTCGTACTTGGTTTGGTCCAGGGAGTAGAGTCATTGTAACATCGAGAGATGAACATTTGCTCAGTGCGTTTGGAGTAGATGGAGTATACAAGGTGAAACCACTAACTGATCCTGAAGCTCTTCAGCTCTTCCATATGAAAGCCTTTAAGAAAGACCAGCTGGTTGGGGAAGACTTTCTCAAGTTATCCAAGGAATTTTTGAAGTATTATAAGGGTCTTCCATTAGCTATTAAAGTTTTGGGATCATCTGTCAATGGGGAAGACATGGATCGTGTAAAAAGAATATTACAAGGTAGTAATGACGATTCTCTAGACTTAGATCTAGAAGTTCTCCTGGAAAAATCTATGGTAATTCTATTTGGAAAAAAGTTGTGGATGCATGATTTGATACAAGAATTGGGTTGGGAACTTGTTCATCAAGAATGTTGTCAAGACCCTGGACAACGTAGCAGGTTGGGGCTTTCCAACGAGATCCTCCATGTATTTGATCGGAATAAGGGAACGAGTGCAGTTCAAAGCATATTCCTCCAACGTCCAACAAAAGACAATGTGCTTCACTCAATCAATGATTCCTTTTCAAATATGGACAGATTGAGATTACTCAGAATTTGGAATGTGAAGTTTTCTGGCGATATCAAATATCTGTCTAATGAGTTACAGTTTCTGGATTGGCATGAATGCCCTCCAGTTTCTGGATTGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

354

Amino Acids

40.19

Weight (kDa)

5.91

Isoelectric Point (pI)

37.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 5 - 171 3.6e-22 NB-ARC domain
NACHT PF05729 22 - 169 1.6e-07 NACHT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 567
Acc36I ACCTGC 1 cut(s) 798
AccB7I CCANNNNNTGG 1 cut(s) 1049
AccI GTMKAC 1 cut(s) 444
AclWI GGATC 6 cut(s) 68, 336, 461, 607, 633, 823
AcsI RAATTY 2 cut(s) 551, 963
AcuI CTGAAG 2 cut(s) 464, 492
AfaI GTAC 3 cut(s) 238, 249, 364
AfiI CCNNNNNNNGG 1 cut(s) 1049
AflIII ACRYGT 1 cut(s) 244
AgsI TTSAA 5 cut(s) 143, 222, 559, 872, 936
AjnI CCWGG 3 cut(s) 376, 690, 793
AluBI AGCT 6 cut(s) 134, 153, 476, 484, 517, 584
AluI AGCT 6 cut(s) 134, 153, 476, 484, 517, 584
AlwI GGATC 6 cut(s) 68, 336, 461, 607, 633, 823
AlwNI CAGNNNCTG 2 cut(s) 1021, 1049
ApeKI GCWGC 1 cut(s) 208
ApoI RAATTY 2 cut(s) 551, 963
Asp700I GAANNNNTTC 3 cut(s) 477, 531, 768
AspS9I GGNCC 1 cut(s) 374
AsuHPI GGTGA 2 cut(s) 158, 463
AvaII GGWCC 1 cut(s) 374
BbsI GAAGAC 3 cut(s) 534, 566, 624
BbvI GCAGC 1 cut(s) 220
BccI CCATC 1 cut(s) 431
BciT130I CCWGG 3 cut(s) 378, 692, 795
BfaI CTAG 2 cut(s) 668, 680
BfuAI ACCTGC 1 cut(s) 798
BglII AGATCT 1 cut(s) 676
BisI GCNGC 1 cut(s) 209
BlsI GCNGC 1 cut(s) 210
BmcAI AGTACT 1 cut(s) 238
Bme1390I CCNGG 3 cut(s) 378, 692, 795
Bme18I GGWCC 1 cut(s) 374
BmgT120I GGNCC 1 cut(s) 374
BmrFI CCNGG 3 cut(s) 378, 692, 795
BmsI GCATC 1 cut(s) 723
BpiI GAAGAC 3 cut(s) 534, 566, 624
BpmI CTGGAG 1 cut(s) 1026
BpuEI CTTGAG 2 cut(s) 179, 521
BsaBI GATNNNNATC 1 cut(s) 327
BsaJI CCNNGG 3 cut(s) 377, 546, 793
Bsc4I CCNNNNNNNGG 1 cut(s) 1049
Bse1I ACTGG 2 cut(s) 255, 1043
Bse8I GATNNNNATC 1 cut(s) 327
BseBI CCWGG 3 cut(s) 378, 692, 795
BseDI CCNNGG 3 cut(s) 377, 546, 793
BseGI GGATG 1 cut(s) 738
BseJI GATNNNNATC 1 cut(s) 327
BseLI CCNNNNNNNGG 1 cut(s) 1049
BseMII CTCAG 4 cut(s) 111, 149, 433, 973
BseNI ACTGG 2 cut(s) 255, 1043
BseXI GCAGC 1 cut(s) 220
BsgI GTGCAG 1 cut(s) 885
BslI CCNNNNNNNGG 1 cut(s) 1049
BsmI GAATGC 1 cut(s) 1040
Bsp1407I TGTACA 1 cut(s) 247
Bsp143I GATC 8 cut(s) 60, 328, 466, 599, 625, 676, 828, 844
BspCNI CTCAG 4 cut(s) 112, 148, 432, 972
BspMI ACCTGC 1 cut(s) 798
BspPI GGATC 6 cut(s) 68, 336, 461, 607, 633, 823
BspQI GCTCTTC 2 cut(s) 483, 491
BsrGI TGTACA 1 cut(s) 247
BsrI ACTGG 2 cut(s) 255, 1043
BssECI CCNNGG 3 cut(s) 377, 546, 793
BssMI GATC 8 cut(s) 60, 328, 466, 599, 625, 676, 828, 844
BssNAI GTATAC 1 cut(s) 445
BssT1I CCWWGG 1 cut(s) 546
Bst1107I GTATAC 1 cut(s) 445
Bst2UI CCWGG 3 cut(s) 378, 692, 795
Bst4CI ACNGT 1 cut(s) 1017
Bst6I CTCTTC 2 cut(s) 483, 491
BstAUI TGTACA 1 cut(s) 247
BstC8I GCNNGC 1 cut(s) 348
BstDEI CTNAG 6 cut(s) 34, 120, 135, 419, 673, 959
BstF5I GGATG 1 cut(s) 738
BstKTI GATC 8 cut(s) 63, 331, 469, 602, 628, 679, 831, 847
BstMBI GATC 8 cut(s) 60, 328, 466, 599, 625, 676, 828, 844
BstNI CCWGG 3 cut(s) 378, 692, 795
BstNSI RCATGY 1 cut(s) 248
BstSCI CCNGG 3 cut(s) 376, 690, 793
BstV1I GCAGC 1 cut(s) 220
BstV2I GAAGAC 3 cut(s) 534, 566, 624
BstX2I RGATCY 2 cut(s) 676, 828
BstXI CCANNNNNNTGG 2 cut(s) 262, 521
BstYI RGATCY 2 cut(s) 676, 828
BstZ17I GTATAC 1 cut(s) 445
BtsCI GGATG 1 cut(s) 738
BtsIMutI CAGTG 1 cut(s) 427
BveI ACCTGC 1 cut(s) 798
Cac8I GCNNGC 1 cut(s) 348
CaiI CAGNNNCTG 2 cut(s) 1021, 1049
Cfr13I GGNCC 1 cut(s) 374
Csp6I GTAC 3 cut(s) 237, 248, 363
CviAII CATG 6 cut(s) 245, 622, 737, 836, 1031, 1059
CviJI RGCY 8 cut(s) 134, 153, 476, 484, 500, 517, 584, 817
CviKI_1 RGCY 8 cut(s) 134, 153, 476, 484, 500, 517, 584, 817
CviQI GTAC 3 cut(s) 237, 248, 363
DdeI CTNAG 6 cut(s) 34, 120, 135, 419, 673, 959
DpnI GATC 8 cut(s) 62, 330, 468, 601, 627, 678, 830, 846
DpnII GATC 8 cut(s) 60, 328, 466, 599, 625, 676, 828, 844
Eam1104I CTCTTC 2 cut(s) 483, 491
EarI CTCTTC 2 cut(s) 483, 491
Eco130I CCWWGG 1 cut(s) 546
Eco32I GATATC 1 cut(s) 991
Eco47I GGWCC 1 cut(s) 374
Eco57I CTGAAG 2 cut(s) 464, 492
EcoRII CCWGG 3 cut(s) 376, 690, 793
EcoRV GATATC 1 cut(s) 991
EcoT14I CCWWGG 1 cut(s) 546
EcoT22I ATGCAT 1 cut(s) 738
ErhI CCWWGG 1 cut(s) 546
FaeI CATG 6 cut(s) 248, 625, 740, 839, 1034, 1062
FatI CATG 6 cut(s) 244, 621, 736, 835, 1030, 1058
FauNDI CATATG 1 cut(s) 492
FblI GTMKAC 1 cut(s) 444
Fnu4HI GCNGC 1 cut(s) 209
FokI GGATG 1 cut(s) 745
Fsp4HI GCNGC 1 cut(s) 209
FspBI CTAG 2 cut(s) 668, 680
GluI GCNGC 1 cut(s) 209
GsuI CTGGAG 1 cut(s) 1026
Hin1II CATG 6 cut(s) 248, 625, 740, 839, 1034, 1062
HinfI GANTC 4 cut(s) 5, 387, 662, 926
HphI GGTGA 2 cut(s) 158, 463
Hpy166II GTNNAC 1 cut(s) 445
Hpy188I TCNGA 5 cut(s) 60, 92, 121, 849, 962
Hpy8I GTNNAC 1 cut(s) 445
HpyAV CCTTC 1 cut(s) 137
HpyCH4III ACNGT 1 cut(s) 1017
HpyCH4IV ACGT 2 cut(s) 802, 889
HpyCH4V TGCA 4 cut(s) 107, 346, 736, 866
HpyF3I CTNAG 6 cut(s) 34, 120, 135, 419, 673, 959
HpySE526I ACGT 2 cut(s) 802, 889
Hsp92II CATG 6 cut(s) 248, 625, 740, 839, 1034, 1062
Kzo9I GATC 8 cut(s) 60, 328, 466, 599, 625, 676, 828, 844
LguI GCTCTTC 2 cut(s) 483, 491
Lsp1109I GCAGC 1 cut(s) 220
LweI GCATC 1 cut(s) 723
MaeI CTAG 2 cut(s) 668, 680
MaeII ACGT 2 cut(s) 802, 889
MaeIII GTNAC 3 cut(s) 175, 394, 1011
MalI GATC 8 cut(s) 62, 330, 468, 601, 627, 678, 830, 846
MboI GATC 8 cut(s) 60, 328, 466, 599, 625, 676, 828, 844
MboII GAAGA 6 cut(s) 299, 470, 478, 539, 566, 629
MfeI CAATTG 1 cut(s) 341
MflI RGATCY 2 cut(s) 676, 828
MluCI AATT 5 cut(s) 341, 551, 708, 752, 963
MlyI GAGTC 1 cut(s) 396
MmeI TCCRAC 4 cut(s) 70, 846, 910, 917
MnlI CCTC 5 cut(s) 117, 166, 842, 893, 1050
Mph1103I ATGCAT 1 cut(s) 738
MroXI GAANNNNTTC 3 cut(s) 477, 531, 768
MseI TTAA 2 cut(s) 504, 588
MslI CAYNNNNRTG 1 cut(s) 15
MspA1I CMGCKG 1 cut(s) 517
MspR9I CCNGG 3 cut(s) 378, 692, 795
MunI CAATTG 1 cut(s) 341
Mva1269I GAATGC 1 cut(s) 1040
MvaI CCWGG 3 cut(s) 378, 692, 795
NdeI CATATG 1 cut(s) 492
NdeII GATC 8 cut(s) 60, 328, 466, 599, 625, 676, 828, 844
NlaIII CATG 6 cut(s) 248, 625, 740, 839, 1034, 1062
NsiI ATGCAT 1 cut(s) 738
NspI RCATGY 1 cut(s) 248
PciI ACATGT 1 cut(s) 244
PciSI GCTCTTC 2 cut(s) 483, 491
PctI GAATGC 1 cut(s) 1040
PdmI GAANNNNTTC 3 cut(s) 477, 531, 768
PfeI GAWTC 3 cut(s) 5, 662, 926
PflMI CCANNNNNTGG 1 cut(s) 1049
PfoI TCCNGGA 1 cut(s) 690
PkrI GCNGC 1 cut(s) 210
PleI GAGTC 1 cut(s) 395
PpsI GAGTC 1 cut(s) 395
PscI ACATGT 1 cut(s) 244
PsiI TTATAA 1 cut(s) 567
Psp6I CCWGG 3 cut(s) 376, 690, 793
PspGI CCWGG 3 cut(s) 376, 690, 793
PspPI GGNCC 1 cut(s) 374
PstNI CAGNNNCTG 2 cut(s) 1021, 1049
PsuI RGATCY 2 cut(s) 676, 828
PvuII CAGCTG 1 cut(s) 517
RsaI GTAC 3 cut(s) 238, 249, 364
RsaNI GTAC 3 cut(s) 237, 248, 363
RseI CAYNNNNRTG 1 cut(s) 15
SapI GCTCTTC 2 cut(s) 483, 491
SaqAI TTAA 2 cut(s) 504, 588
SatI GCNGC 1 cut(s) 209
Sau3AI GATC 8 cut(s) 60, 328, 466, 599, 625, 676, 828, 844
Sau96I GGNCC 1 cut(s) 374
ScaI AGTACT 1 cut(s) 238
SchI GAGTC 1 cut(s) 396
ScrFI CCNGG 3 cut(s) 378, 692, 795
SfaNI GCATC 1 cut(s) 723
SinI GGWCC 1 cut(s) 374
SmiMI CAYNNNNRTG 1 cut(s) 15
SmlI CTYRAG 2 cut(s) 158, 536
SmoI CTYRAG 2 cut(s) 158, 536
Sse9I AATT 5 cut(s) 341, 551, 708, 752, 963
SspI AATATT 1 cut(s) 642
SspMI CTAG 2 cut(s) 668, 680
StyD4I CCNGG 3 cut(s) 376, 690, 793
StyI CCWWGG 1 cut(s) 546
TaaI ACNGT 1 cut(s) 1017
TaiI ACGT 2 cut(s) 805, 892
TaqI TCGA 1 cut(s) 401
TasI AATT 5 cut(s) 341, 551, 708, 752, 963
TatI WGTACW 2 cut(s) 236, 247
TfiI GAWTC 3 cut(s) 5, 662, 926
Tru1I TTAA 2 cut(s) 504, 588
Tru9I TTAA 2 cut(s) 504, 588
TscAI CASTG 1 cut(s) 427
TseI GCWGC 1 cut(s) 208
TspDTI ATGAA 7 cut(s) 18, 33, 188, 423, 509, 761, 1047
TspRI CASTG 1 cut(s) 427
Van91I CCANNNNNTGG 1 cut(s) 1049
VpaK11BI GGWCC 1 cut(s) 374
XapI RAATTY 2 cut(s) 551, 963
XbaI TCTAGA 2 cut(s) 667, 679
XceI RCATGY 1 cut(s) 248
XmiI GTMKAC 1 cut(s) 444
XmnI GAANNNNTTC 3 cut(s) 477, 531, 768
XspI CTAG 2 cut(s) 668, 680
ZrmI AGTACT 1 cut(s) 238
Zsp2I ATGCAT 1 cut(s) 738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.