Rh1BG151400

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0000129
Physical Location & Seq
Forward (+)
24889430 .. 24898343
8914 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG151400.1

Sequence Viewer

Length: 4278 bp
ATGGAATCCCACATGAAGAATATGCTTTCAGTCATGGAGATAGGATTGCCTGATGTACGGATTATAGGGATTTGGGGAATGGGTGGTATCGGTAAGACAACTATTGCCCAAGAAGTTTCTAAAGGGGTACGTGCTGGGTTTGAAGGTTACAGCTTTCTTGCCAATGTTAGAGAGGAATTTGAAAAGCAAGGGGCAGTTCATTTACAAAAGCAACTTCTTTCAGACTTGCTCAGAAATAATGTAAATGTACAGAACATAGATATGGAAGAGGTATTGTGTGTTAGGCTACGAACTAAAATGGTTCTTATCGTTCTTGATGACGTCAATCAGTTAGAAGAATTGGAGGCATTGTGTGATCGTACATGGTTTGGTTCAGGGAGTAGAATCATCATAACATCCAGAAATGAACATTTGTTGAATGGATTTGGAGTAGACAAAATATACCAGGTTAACACATTAACTGATGATGAAGCTCTCCAGCTTTTTAGTTTGAAAGCATTCAAGAAAGACGTGGTTGAAGAAGACTTTCTCAACCTATCCATGAAATTTTTAAAATATGCTAACGGCCTTCCATTGGCTATTAAAGTTTTAGGTTCATCTCTTCATGGCAGAAGCCTAAAACTCTGGTCGAGTACATTGGATAGATTAAAAGAAAATCCTGAAAAAAAAATTATTGATGTGCTTAAAGTAAGTTTTGATGGATTACGAGAAACAGAAAAGAAAACATTTTTGGATATTGCATGTTTCTTTGAAGGTGAGGACAAAGATCGTGTAACAGGAGTACTAGAAAGTCGTGGCTACTGTCCTGACATTGATATAGAAGTTCTCATGGAGAAATCTCTGGTAACTCTATTTGGAAGAAAGTTGCGAATGCATGATTTGATACAAGAATTGGGTTGGGAAATTGTTCGTCAAGAGTGTCGCGAAGATGCGTGCAAACGTAGCAGATTGTGGCTTCCCAAGGATATCATCCATTTACTTGAAAATAATGAGGGTACGACTACAGTGGAAGCTATACTCCTGAGTTTGCCGAAACATATACACCAGAAGGTTCAAATGGATGTCGATCCCTTCTCAAAGATGTACAAATTAAGATTGCAGAAAATTAGCAATGTGTACTTTAGTGGAAATGTCAAATTTCTTTCTAATGAGTTACAATTTCTGGAATGGCATGCATGTCCCTTAAACTCTTTTCCACCAAACTTTCAATCAGATAAGCTTGTTGAAATCAGCATGCATTCGAGCCGTCTCAAACAACTATGGAATGGAAATGAGAGCTGGAGTATGCTAAAGTCCATCGATCTGAGTTTTTCCCAATACTTGATCAAGACCCCAAACTTCACCAAGGTTCCAAATCTTGAGATGTTGGTTCTTGAAGGTTGCAAAAAGTTGGTTGAGGTTCACCCATCCATTGGATTTCTTAGAAAGCTTGTTTTGTTGAATATGGAGAATTGTGAATCTATTGAGAGCCTTCCGCCTTTCATTAGATTGGAATCTCTTCAAAGATTTACTCTCTCTGGTTGTTTGAAACTCAAGAGGTTTCCTGAAATTGAAGGAAACATGAAAAGCTTGTTAGAGCTTCAATTAGGCGGGATTGCTATAGAGGAATTACCTCCTTCAATCGAATGTTTGAGTGGTCTTACTTTATTGAACTTAAGTTACTGTAGAAACCTTTTGCGTCTCCCCTGCACCATTCAGTTTCTGACGTCTCTGAAAAGTCTCATTCTAACAGGTTGCTCAGAGCTTGATCATATTCCTGAGAATTTGAAGTGTGTGGAATGCCTAGAGGAGCTTGATATAAGTGGGACTGCTATAAGAGGTTCATCTTTCATTGTTGGTATGAATAATCTGAAGTACCTATCTTTCCGTGGATGTAAAGATCAACCTACAAAGCCATGGTATTCATTCCTTGATTGTTTGTGGGGTGGAAAGAGCCATGTTCCCAGGAGTTTGTTGTTGCCTGCTTCAATGTCAAGCCTAACTTCTTTGACAGAGCTAGACTTAAGTAATTGCAACCTGATGGACGGATCAATTCCAAATGGTCTTGGCAGCTTATTCTCCTTAAAAACTTTAAAGTTGAGTGATAACAATTTTATTCTGTTACCGGAAAGCATATCTCAACTCTCCAAGCTTAGAGTACTGCACTTGAACAATTGTAGCAGACTTCAGTCGCTGCCTAAGCTTCCATTAAGTGTTCGACATGTGGATGCAAAAGATTGTACTTCACTGAAGGATTTCCCCAATCAATACAAAGTATGGACTTCATGTGTCTCAGCAATGACTACTGTCAATTCTCTCGTTTCACCCAAGAATCAAGAACCTTGTACTTCAGTGCAGAGTGATTTCTTTAGCGTATGGACCAGCTCTGCAGACTCAGGAATAAGTTTTAGTATGACATCTGAGCAGCTTCCAGATTGGAATCCATTCGTTGATGCAGTGCCAGCACAAATTTTTCAAAAAGACCTTGAACTATTGGATTATCGGTCAACTCCTTTGCAGGCAGTTCTTCATCGAACTGAAATTCCAGAGTTGTTCAGCCATTTGGCCACTGGGAATTTCCTTGCAATTCCACTACCCCCAGAATTGAGTGAGGATGGCAAGTGGGTTGGTATTGCTGTTGTTTTCCTAGTCAAGAGACATCCCGGTCTTTCCTACATTGAATCTGAATCAGAAGCTTCTAACTACTTCTATAGATTTGACATTGGAAGTCGAGAATTTCAACTCAAACCAAACCTCATTAGTGACTGGAGATTGCGTCACATGTTTGTTGGAACTAGTTTTGATAATTTTCTTTGCGTTTTCTACGTACCTTGTCTGATGTTTCCAAAGATGCTGAATGAATCAACTGTGATAGGCGCAATATTTCAAACCAACAACCCATGCATGGAGGTCCAGACATGTGGGATGCGTCTAATTTACGAGCAAGAAGTTCCAAGGTTTATCCAAACATTTATTCCGTTTAGCATAGGCCACCAGCATGAAATCCCTTCTCATGTTGATAAAGCTACACTTGAATCTGGATGGTTTAATCTTGTGGATAATATTTTGAGATGGGAGAAATCAATTCCACAGTCCCAAGAAGCCTCCACTGTTTTGCTCAGAAGGAACATCGAGTCAGTTCTACCAAGATACTTTGAGGGACTAAACCATCAGCTTAAAGTATATGATTTTTTTGTAAGAGGAAGCCCGGCATGGTTCAATCCTCAAGTTGGAACCTCTGCATCTATCAGTCTGCCTCATAATCTACTCAAAAGTAAGAAGTGGATGGGGTTTGTTGCATGTATGTATGTGGATCGACATAAACTTGTCAAGGACATCTATTATCTACATTTTGAACTCCAAAGGGAGGATTTTCGTAGCTTACCGTTACAGCTGTTTAATGTATCAAGGTACAACACCTTTGATAGTGATCTTTTGGTTGTTTTCATACCACGTGCACAAATTCCTGAAGATTTCACTACTTCAAGTCAGATCCGCATCTCCTTCTTGAGAACTGATAGCCCTGATTATGTAGAGGTTCAAACATGTGGGTTACGTATTTTGTATCAGGAAGATTTACAAAGCTTTGTCCAGGTAATCATACAGTGCATATTACGCTGCTCACCCACAGTCTTTGGATATTATAACCAATTAGTGGTCGAAGATTGGCTAGACTTCAAAAGATTTCCAGAGCGTCATGACAGAAAATTCAGTACCGATATAGAATCCCGGCCTTTACAGTTCTCACAATATCTTAGAGACCGGGACTGGTCTGCGGATATGTTTAATCTTAAGTTCCCTGGAATTGAAATTCCGAAGTGGTTCATGCATCCGAATAAAGGCAACTATGCACAACTCCAACTACCTCCAAATATTTTCAATGATGATAATTGGATTGGTATTGCTGTGTGTTCTTCTTTATCAATCCACAAACACCCAAACATCCTTCTTGACACTCCTGCTGCACAGTATACTCATGAACTTTTGTTTTATCTTAACAACGATGTTGGTAATGCCCCTACCTGTTTACGGACATATATAAAGGAATCCATGTGGTTGCATGAATGTGATTTCACTTGGTTCAACTATGTAGCGCGTTCTATATTTCCTGACTCATGGAATCAATCCAATCGCATTGATGCAACTGTTGAATCCAATAACCCATGCATAGGAAAGCATGGATGTGCACTTCGTCTGGTATTCAAGGAGGATGTTGAAGAGCTTGTACAAATGTTGAACCTCTGTGAATCATCTCAAAAGTGCAGTCATTGCGAAGAAGCTTTAAAAAATGGTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1425

Amino Acids

163.34

Weight (kDa)

5.94

Isoelectric Point (pI)

49.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 17 - 170 1.4e-22 NB-ARC domain
NACHT PF05729 22 - 167 2e-06 NACHT domain
WHD_ROQ1 PF23282 237 - 305 5.8e-16 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 497 - 583 2.4e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 527 - 620 7.2e-06 Leucine-rich repeat region
LRR_14 PF23598 655 - 727 1.3e-07 Leucine-rich repeat region
C-JID PF20160 1063 - 1186 3.5e-07 C-JID domain
C-JID PF20160 1259 - 1399 9.8e-10 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3624
AasI GACNNNNNNGTC 1 cut(s) 2643
AatII GACGTC 2 cut(s) 324, 1709
AccB7I CCANNNNNTGG 2 cut(s) 1412, 3634
AccI GTMKAC 2 cut(s) 432, 3950
AccII CGCG 2 cut(s) 924, 4075
AciI CCGC 4 cut(s) 1475, 1590, 3475, 3755
AclWI GGATC 4 cut(s) 1061, 2035, 3300, 3466
AcoI YGGCCR 1 cut(s) 2544
AcuI CTGAAG 5 cut(s) 1871, 2150, 2249, 2313, 3468
AcvI CACGTG 1 cut(s) 3434
AcyI GRCGYC 2 cut(s) 321, 1706
AfiI CCNNNNNNNGG 9 cut(s) 574, 1412, 2884, 3209, 3346, 3634, 3818, 4008, 4148
AflII CTTAAG 3 cut(s) 1654, 2002, 3770
AflIII ACRYGT 4 cut(s) 2200, 2760, 2897, 3524
AhlI ACTAGT 1 cut(s) 2774
AjiI CACGTC 1 cut(s) 511
AjnI CCWGG 4 cut(s) 444, 1943, 3570, 3778
AjuI GAANNNNNNNTTGG 4 cut(s) 713, 745, 2937, 2969
AloI GAACNNNNNNTCC 2 cut(s) 2506, 2538
Alw21I GWGCWC 2 cut(s) 3439, 4168
Alw26I GTCTC 7 cut(s) 1253, 1685, 1713, 1724, 2275, 2629, 3732
Alw44I GTGCAC 2 cut(s) 3435, 4164
AlwI GGATC 4 cut(s) 1061, 2035, 3300, 3466
AlwNI CAGNNNCTG 2 cut(s) 1702, 2173
AoxI GGCC 4 cut(s) 565, 2544, 2968, 3710
ApaLI GTGCAC 2 cut(s) 3435, 4164
ApeKI GCWGC 5 cut(s) 2049, 2173, 2404, 3597, 3941
ArsI GACNNNNNNTTYG 2 cut(s) 4228, 4260
Asp700I GAANNNNTTC 7 cut(s) 497, 525, 906, 1497, 2234, 2423, 3800
AspLEI GCGC 2 cut(s) 2858, 4075
AspS9I GGNCC 2 cut(s) 2358, 2890
AsuC2I CCSGG 4 cut(s) 2643, 3188, 3709, 3743
AsuHPI GGTGA 5 cut(s) 767, 1333, 1394, 2295, 3594
AvaII GGWCC 2 cut(s) 2358, 2890
BaeGI GKGCMC 2 cut(s) 3439, 4168
BalI TGGCCA 1 cut(s) 2546
BarI GAAGNNNNNNTAC 2 cut(s) 4188, 4220
BbrPI CACGTG 1 cut(s) 3434
BbsI GAAGAC 1 cut(s) 528
Bbv12I GWGCWC 2 cut(s) 3439, 4168
BbvI GCAGC 5 cut(s) 2061, 2160, 2416, 3584, 3928
BccI CCATC 9 cut(s) 692, 1304, 1414, 2014, 2588, 3015, 3045, 3156, 3259
BceAI ACGGC 2 cut(s) 580, 1230
BciT130I CCWGG 4 cut(s) 446, 1945, 3572, 3780
BclI TGATCA 2 cut(s) 1323, 1747
BcnI CCSGG 4 cut(s) 2643, 3188, 3709, 3743
BcoDI GTCTC 7 cut(s) 1253, 1685, 1713, 1724, 2275, 2629, 3732
BcuI ACTAGT 1 cut(s) 2774
BfaI CTAG 6 cut(s) 785, 1784, 1997, 2627, 2775, 3650
BfmI CTRYAG 5 cut(s) 1002, 1599, 1663, 2367, 2689
BfrI CTTAAG 3 cut(s) 1654, 2002, 3770
BisI GCNGC 5 cut(s) 2050, 2174, 2405, 3598, 3942
BlsI GCNGC 5 cut(s) 2051, 2175, 2406, 3599, 3943
BmcAI AGTACT 2 cut(s) 783, 2139
Bme1390I CCNGG 8 cut(s) 446, 1945, 2643, 3188, 3572, 3709, 3743, 3780
Bme18I GGWCC 2 cut(s) 2358, 2890
BmgBI CACGTC 1 cut(s) 511
BmgT120I GGNCC 2 cut(s) 2358, 2890
BmiI GGNNCC 2 cut(s) 1350, 3214
BmrFI CCNGG 8 cut(s) 446, 1945, 2643, 3188, 3572, 3709, 3743, 3780
BmrI ACTGGG 1 cut(s) 2559
BmsI GCATC 9 cut(s) 919, 2197, 2422, 2820, 2895, 3230, 3486, 3817, 4108
BmuI ACTGGG 1 cut(s) 2559
BoxI GACNNNNGTC 2 cut(s) 2167, 2285
BpiI GAAGAC 1 cut(s) 528
BpmI CTGGAG 3 cut(s) 461, 1300, 2767
Bpu10I CCTNAGC 1 cut(s) 2178
BpuEI CTTGAG 4 cut(s) 1379, 1517, 3189, 3508
BpuMI CCSGG 4 cut(s) 2643, 3188, 3709, 3743
Bsa29I ATCGAT 1 cut(s) 1299
BsaAI YACGTR 4 cut(s) 131, 2806, 3434, 3536
BsaBI GATNNNNATC 5 cut(s) 1065, 1492, 2418, 3408, 3476
BsaHI GRCGYC 2 cut(s) 321, 1706
BsaI GGTCTC 1 cut(s) 3732
BsaJI CCNNGG 7 cut(s) 960, 1344, 1867, 1895, 1943, 2933, 3778
BsaWI WCCGGW 1 cut(s) 2104
BsaXI ACNNNNNCTCC 4 cut(s) 335, 365, 2740, 2770
Bsc4I CCNNNNNNNGG 9 cut(s) 574, 1412, 2884, 3209, 3346, 3634, 3818, 4008, 4148
Bse1I ACTGG 3 cut(s) 2554, 2750, 3752
Bse3DI GCAATG 3 cut(s) 1117, 2283, 4246
Bse8I GATNNNNATC 5 cut(s) 1065, 1492, 2418, 3408, 3476
BseBI CCWGG 4 cut(s) 446, 1945, 3572, 3780
BseCI ATCGAT 1 cut(s) 1299
BseDI CCNNGG 7 cut(s) 960, 1344, 1867, 1895, 1943, 2933, 3778
BseJI GATNNNNATC 5 cut(s) 1065, 1492, 2418, 3408, 3476
BseLI CCNNNNNNNGG 9 cut(s) 574, 1412, 2884, 3209, 3346, 3634, 3818, 4008, 4148
BseMI GCAATG 3 cut(s) 1117, 2283, 4246
BseMII CTCAG 9 cut(s) 244, 1013, 1295, 1749, 1752, 2286, 2388, 2391, 3112
BseNI ACTGG 3 cut(s) 2554, 2750, 3752
BseRI GAGGAG 1 cut(s) 1802
BseSI GKGCMC 2 cut(s) 3439, 4168
BseXI GCAGC 5 cut(s) 2061, 2160, 2416, 3584, 3928
BseYI CCCAGC 1 cut(s) 134
BsgI GTGCAG 5 cut(s) 1672, 2126, 2354, 3927, 4261
Bsh1236I CGCG 2 cut(s) 924, 4075
BshFI GGCC 4 cut(s) 567, 2546, 2970, 3712
BshVI ATCGAT 1 cut(s) 1299
BsiHKAI GWGCWC 2 cut(s) 3439, 4168
BsiSI CCGG 5 cut(s) 2105, 2643, 3188, 3709, 3742
BslFI GGGAC 5 cut(s) 1164, 1819, 3058, 3153, 3758
BslI CCNNNNNNNGG 9 cut(s) 574, 1412, 2884, 3209, 3346, 3634, 3818, 4008, 4148
BsmAI GTCTC 7 cut(s) 1253, 1685, 1713, 1724, 2275, 2629, 3732
BsmBI CGTCTC 3 cut(s) 1253, 1685, 1713
BsmFI GGGAC 5 cut(s) 1164, 1819, 3058, 3153, 3758
BsmI GAATGC 4 cut(s) 497, 876, 1237, 1784
BsnI GGCC 4 cut(s) 567, 2546, 2970, 3712
Bso31I GGTCTC 1 cut(s) 3732
Bsp1286I GDGCHC 2 cut(s) 3439, 4168
Bsp1407I TGTACA 3 cut(s) 247, 1083, 4204
Bsp19I CCATGG 1 cut(s) 1895
Bsp68I TCGCGA 1 cut(s) 924
BspACI CCGC 4 cut(s) 1475, 1590, 3475, 3755
BspANI GGCC 4 cut(s) 567, 2546, 2970, 3712
BspCNI CTCAG 9 cut(s) 243, 1014, 1296, 1750, 1751, 2285, 2387, 2392, 3111
BspDI ATCGAT 1 cut(s) 1299
BspFNI CGCG 2 cut(s) 924, 4075
BspHI TCATGA 2 cut(s) 3676, 3955
BspLI GGNNCC 2 cut(s) 1350, 3214
BspMAI CTGCAG 1 cut(s) 2371
BspPI GGATC 4 cut(s) 1061, 2035, 3300, 3466
BspQI GCTCTTC 1 cut(s) 4191
BspTI CTTAAG 3 cut(s) 1654, 2002, 3770
BspTNI GGTCTC 1 cut(s) 3732
BsrDI GCAATG 3 cut(s) 1117, 2283, 4246
BsrGI TGTACA 3 cut(s) 247, 1083, 4204
BsrI ACTGG 3 cut(s) 2554, 2750, 3752
BssECI CCNNGG 7 cut(s) 960, 1344, 1867, 1895, 1943, 2933, 3778
BssNAI GTATAC 1 cut(s) 3951
BssNI GRCGYC 2 cut(s) 321, 1706
BssT1I CCWWGG 4 cut(s) 960, 1344, 1895, 2933
Bst1107I GTATAC 1 cut(s) 3951
Bst2UI CCWGG 4 cut(s) 446, 1945, 3572, 3780
Bst6I CTCTTC 4 cut(s) 261, 606, 1503, 4191
BstACI GRCGYC 2 cut(s) 321, 1706
BstAFI CTTAAG 3 cut(s) 1654, 2002, 3770
BstAPI GCANNNNNTGC 1 cut(s) 4248
BstAUI TGTACA 3 cut(s) 247, 1083, 4204
BstBAI YACGTR 4 cut(s) 131, 2806, 3434, 3536
BstC8I GCNNGC 6 cut(s) 934, 1173, 1235, 1962, 2442, 2499
BstDSI CCRYGG 2 cut(s) 1867, 1895
BstFNI CGCG 2 cut(s) 924, 4075
BstHHI GCGC 2 cut(s) 2858, 4075
BstMAI GTCTC 7 cut(s) 1253, 1685, 1713, 1724, 2275, 2629, 3732
BstMWI GCNNNNNNNGC 5 cut(s) 942, 2179, 2441, 3594, 4248
BstNI CCWGG 4 cut(s) 446, 1945, 3572, 3780
BstNSI RCATGY 9 cut(s) 744, 1175, 1179, 1237, 2204, 2764, 2901, 3282, 3528
BstPAI GACNNNNGTC 2 cut(s) 2167, 2285
BstSCI CCNGG 8 cut(s) 444, 1943, 2641, 3186, 3570, 3707, 3741, 3778
BstSFI CTRYAG 5 cut(s) 1002, 1599, 1663, 2367, 2689
BstSLI GKGCMC 2 cut(s) 3439, 4168
BstSNI TACGTA 2 cut(s) 2806, 3536
BstUI CGCG 2 cut(s) 924, 4075
BstV1I GCAGC 5 cut(s) 2061, 2160, 2416, 3584, 3928
BstV2I GAAGAC 1 cut(s) 528
BstX2I RGATCY 1 cut(s) 3471
BstXI CCANNNNNNTGG 1 cut(s) 2900
BstYI RGATCY 1 cut(s) 3471
BstZ17I GTATAC 1 cut(s) 3951
Bsu15I ATCGAT 1 cut(s) 1299
BsuRI GGCC 4 cut(s) 567, 2546, 2970, 3712
BsuTUI ATCGAT 1 cut(s) 1299
BtgI CCRYGG 2 cut(s) 1867, 1895
BtrI CACGTC 1 cut(s) 511
BtsI GCAGTG 1 cut(s) 2442
BtsIMutI CAGTG 7 cut(s) 1011, 2225, 2337, 2442, 2547, 3087, 3590
BtuMI TCGCGA 1 cut(s) 924
Cac8I GCNNGC 6 cut(s) 934, 1173, 1235, 1962, 2442, 2499
CaiI CAGNNNCTG 2 cut(s) 1702, 2173
CciI TCATGA 2 cut(s) 3676, 3955
CfoI GCGC 2 cut(s) 2858, 4075
Cfr13I GGNCC 2 cut(s) 2358, 2890
ClaI ATCGAT 1 cut(s) 1299
CseI GACGC 4 cut(s) 1667, 2744, 2897, 3662
CsiI ACCWGGT 1 cut(s) 444
CspCI CAANNNNNGTGG 2 cut(s) 3595, 3630
DraI TTTAAA 3 cut(s) 552, 2073, 4263
DrdI GACNNNNNNGTC 1 cut(s) 2643
DseDI GACNNNNNNGTC 1 cut(s) 2643
EaeI YGGCCR 1 cut(s) 2544
Eam1104I CTCTTC 4 cut(s) 261, 606, 1503, 4191
EarI CTCTTC 4 cut(s) 261, 606, 1503, 4191
EciI GGCGGA 1 cut(s) 1464
Eco105I TACGTA 2 cut(s) 2806, 3536
Eco130I CCWWGG 4 cut(s) 960, 1344, 1895, 2933
Eco31I GGTCTC 1 cut(s) 3732
Eco32I GATATC 1 cut(s) 967
Eco47I GGWCC 2 cut(s) 2358, 2890
Eco57I CTGAAG 5 cut(s) 1871, 2150, 2249, 2313, 3468
Eco72I CACGTG 1 cut(s) 3434
EcoRII CCWGG 4 cut(s) 444, 1943, 3570, 3778
EcoRV GATATC 1 cut(s) 967
EcoT14I CCWWGG 4 cut(s) 960, 1344, 1895, 2933
EcoT22I ATGCAT 6 cut(s) 876, 1177, 1239, 2885, 3810, 4148
ErhI CCWWGG 4 cut(s) 960, 1344, 1895, 2933
Esp3I CGTCTC 3 cut(s) 1253, 1685, 1713
FalI AAGNNNNNCTT 4 cut(s) 1966, 1998, 2994, 3026
FaqI GGGAC 5 cut(s) 1164, 1819, 3058, 3153, 3758
FauI CCCGC 1 cut(s) 1583
FbaI TGATCA 2 cut(s) 1323, 1747
FblI GTMKAC 2 cut(s) 432, 3950
Fnu4HI GCNGC 5 cut(s) 2050, 2174, 2405, 3598, 3942
Fsp4HI GCNGC 5 cut(s) 2050, 2174, 2405, 3598, 3942
FspBI CTAG 6 cut(s) 785, 1784, 1997, 2627, 2775, 3650
GlaI GCGC 2 cut(s) 2857, 4074
GluI GCNGC 5 cut(s) 2050, 2174, 2405, 3598, 3942
GsaI CCCAGC 1 cut(s) 138
GsuI CTGGAG 3 cut(s) 461, 1300, 2767
HaeIII GGCC 4 cut(s) 567, 2546, 2970, 3712
HapII CCGG 5 cut(s) 2105, 2643, 3188, 3709, 3742
HgaI GACGC 4 cut(s) 1667, 2744, 2897, 3662
HhaI GCGC 2 cut(s) 2858, 4075
Hin1I GRCGYC 2 cut(s) 321, 1706
Hin6I GCGC 2 cut(s) 2856, 4073
HinP1I GCGC 2 cut(s) 2856, 4073
HincII GTYRAC 2 cut(s) 451, 2487
HindII GTYRAC 2 cut(s) 451, 2487
HindIII AAGCTT 8 cut(s) 1217, 1427, 1567, 2129, 2180, 2673, 3562, 4257
HpaI GTTAAC 1 cut(s) 451
HpaII CCGG 5 cut(s) 2105, 2643, 3188, 3709, 3742
HphI GGTGA 5 cut(s) 767, 1333, 1394, 2295, 3594
Hpy166II GTNNAC 9 cut(s) 433, 451, 1117, 1402, 2487, 3437, 3951, 4007, 4166
Hpy8I GTNNAC 9 cut(s) 433, 451, 1117, 1402, 2487, 3437, 3951, 4007, 4166
HpyCH4IV ACGT 8 cut(s) 130, 321, 510, 940, 1706, 2805, 3433, 3535
HpyF10VI GCNNNNNNNGC 5 cut(s) 942, 2179, 2441, 3594, 4248
HpySE526I ACGT 8 cut(s) 130, 321, 510, 940, 1706, 2805, 3433, 3535
Hsp92I GRCGYC 2 cut(s) 321, 1706
HspAI GCGC 2 cut(s) 2856, 4073
Ksp22I TGATCA 2 cut(s) 1323, 1747
KspAI GTTAAC 1 cut(s) 451
LguI GCTCTTC 1 cut(s) 4191
LmnI GCTCC 1 cut(s) 1789
Lsp1109I GCAGC 5 cut(s) 2061, 2160, 2416, 3584, 3928
LweI GCATC 9 cut(s) 919, 2197, 2422, 2820, 2895, 3230, 3486, 3817, 4108
MabI ACCWGGT 1 cut(s) 444
MaeI CTAG 6 cut(s) 785, 1784, 1997, 2627, 2775, 3650
MaeII ACGT 8 cut(s) 130, 321, 510, 940, 1706, 2805, 3433, 3535
MfeI CAATTG 1 cut(s) 2152
MflI RGATCY 1 cut(s) 3471
MhlI GDGCHC 2 cut(s) 3439, 4168
MlsI TGGCCA 1 cut(s) 2546
MluNI TGGCCA 1 cut(s) 2546
MlyI GAGTC 3 cut(s) 2366, 3122, 4085
MmeI TCCRAC 3 cut(s) 2749, 3190, 3862
Mox20I TGGCCA 1 cut(s) 2546
Mph1103I ATGCAT 6 cut(s) 876, 1177, 1239, 2885, 3810, 4148
MroXI GAANNNNTTC 7 cut(s) 497, 525, 906, 1497, 2234, 2423, 3800
MscI TGGCCA 1 cut(s) 2546
MslI CAYNNNNRTG 5 cut(s) 260, 2205, 2976, 4044, 4161
Msp20I TGGCCA 1 cut(s) 2546
MspA1I CMGCKG 1 cut(s) 3373
MspCI CTTAAG 3 cut(s) 1654, 2002, 3770
MspI CCGG 5 cut(s) 2105, 2643, 3188, 3709, 3742
MspR9I CCNGG 8 cut(s) 446, 1945, 2643, 3188, 3572, 3709, 3743, 3780
MunI CAATTG 1 cut(s) 2152
Mva1269I GAATGC 4 cut(s) 497, 876, 1237, 1784
MvaI CCWGG 4 cut(s) 446, 1945, 3572, 3780
MvnI CGCG 2 cut(s) 924, 4075
MwoI GCNNNNNNNGC 5 cut(s) 942, 2179, 2441, 3594, 4248
NciI CCSGG 4 cut(s) 2643, 3188, 3709, 3743
NcoI CCATGG 1 cut(s) 1895
NlaIV GGNNCC 2 cut(s) 1350, 3214
NmuCI GTSAC 2 cut(s) 2741, 2756
NruI TCGCGA 1 cut(s) 924
NsiI ATGCAT 6 cut(s) 876, 1177, 1239, 2885, 3810, 4148
NspI RCATGY 9 cut(s) 744, 1175, 1179, 1237, 2204, 2764, 2901, 3282, 3528
PaeI GCATGC 2 cut(s) 1175, 1237
PagI TCATGA 2 cut(s) 3676, 3955
PciI ACATGT 4 cut(s) 2200, 2760, 2897, 3524
PciSI GCTCTTC 1 cut(s) 4191
PctI GAATGC 4 cut(s) 497, 876, 1237, 1784
PdmI GAANNNNTTC 7 cut(s) 497, 525, 906, 1497, 2234, 2423, 3800
PflMI CCANNNNNTGG 2 cut(s) 1412, 3634
PkrI GCNGC 5 cut(s) 2051, 2175, 2406, 3599, 3943
PleI GAGTC 3 cut(s) 2366, 3121, 4085
PmaCI CACGTG 1 cut(s) 3434
PmlI CACGTG 1 cut(s) 3434
PpsI GAGTC 3 cut(s) 2366, 3121, 4085
Ppu21I YACGTR 4 cut(s) 131, 2806, 3434, 3536
PscI ACATGT 4 cut(s) 2200, 2760, 2897, 3524
PshAI GACNNNNGTC 2 cut(s) 2167, 2285
PsiI TTATAA 1 cut(s) 3624
Psp6I CCWGG 4 cut(s) 444, 1943, 3570, 3778
PspCI CACGTG 1 cut(s) 3434
PspFI CCCAGC 1 cut(s) 134
PspGI CCWGG 4 cut(s) 444, 1943, 3570, 3778
PspN4I GGNNCC 2 cut(s) 1350, 3214
PspPI GGNCC 2 cut(s) 2358, 2890
PsrI GAACNNNNNNTAC 2 cut(s) 1643, 1675
PstI CTGCAG 1 cut(s) 2371
PstNI CAGNNNCTG 2 cut(s) 1702, 2173
PsuI RGATCY 1 cut(s) 3471
PvuII CAGCTG 1 cut(s) 3373
RruI TCGCGA 1 cut(s) 924
RseI CAYNNNNRTG 5 cut(s) 260, 2205, 2976, 4044, 4161
SapI GCTCTTC 1 cut(s) 4191
SatI GCNGC 5 cut(s) 2050, 2174, 2405, 3598, 3942
Sau96I GGNCC 2 cut(s) 2358, 2890
ScaI AGTACT 2 cut(s) 783, 2139
SchI GAGTC 3 cut(s) 2366, 3122, 4085
ScrFI CCNGG 8 cut(s) 446, 1945, 2643, 3188, 3572, 3709, 3743, 3780
SduI GDGCHC 2 cut(s) 3439, 4168
SexAI ACCWGGT 1 cut(s) 444
SfaNI GCATC 9 cut(s) 919, 2197, 2422, 2820, 2895, 3230, 3486, 3817, 4108
SfcI CTRYAG 5 cut(s) 1002, 1599, 1663, 2367, 2689
SinI GGWCC 2 cut(s) 2358, 2890
SmiMI CAYNNNNRTG 5 cut(s) 260, 2205, 2976, 4044, 4161
SmlI CTYRAG 7 cut(s) 1358, 1532, 1654, 2002, 3204, 3487, 3770
SmoI CTYRAG 7 cut(s) 1358, 1532, 1654, 2002, 3204, 3487, 3770
SnaBI TACGTA 2 cut(s) 2806, 3536
SpeI ACTAGT 1 cut(s) 2774
SphI GCATGC 2 cut(s) 1175, 1237
SsiI CCGC 4 cut(s) 1475, 1590, 3475, 3755
SspI AATATT 3 cut(s) 2862, 3043, 3853
SspMI CTAG 6 cut(s) 785, 1784, 1997, 2627, 2775, 3650
StyD4I CCNGG 8 cut(s) 444, 1943, 2641, 3186, 3570, 3707, 3741, 3778
StyI CCWWGG 4 cut(s) 960, 1344, 1895, 2933
TaiI ACGT 8 cut(s) 133, 324, 513, 943, 1709, 2808, 3436, 3538
TaqII GACCGA 1 cut(s) 2472
TatI WGTACW 9 cut(s) 247, 632, 781, 1083, 1116, 2137, 2219, 2324, 4204
TscAI CASTG 7 cut(s) 1011, 2232, 2337, 2442, 2554, 3094, 3590
TseFI GTSAC 2 cut(s) 2741, 2756
TseI GCWGC 5 cut(s) 2049, 2173, 2404, 3597, 3941
Tsp45I GTSAC 2 cut(s) 2741, 2756
TspGWI ACGGA 5 cut(s) 73, 1856, 2040, 2946, 4024
TspRI CASTG 7 cut(s) 1011, 2232, 2337, 2442, 2554, 3094, 3590
Van91I CCANNNNNTGG 2 cut(s) 1412, 3634
Vha464I CTTAAG 3 cut(s) 1654, 2002, 3770
VneI GTGCAC 2 cut(s) 3435, 4164
VpaK11BI GGWCC 2 cut(s) 2358, 2890
XceI RCATGY 9 cut(s) 744, 1175, 1179, 1237, 2204, 2764, 2901, 3282, 3528
XcmI CCANNNNNNNNNTGG 1 cut(s) 2546
XmiI GTMKAC 2 cut(s) 432, 3950
XmnI GAANNNNTTC 7 cut(s) 497, 525, 906, 1497, 2234, 2423, 3800
XspI CTAG 6 cut(s) 785, 1784, 1997, 2627, 2775, 3650
ZraI GACGTC 2 cut(s) 322, 1707
ZrmI AGTACT 2 cut(s) 783, 2139
Zsp2I ATGCAT 6 cut(s) 876, 1177, 1239, 2885, 3810, 4148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.