Rw7G041480

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000129
Physical Location & Seq
Forward (+)
64810937 .. 64816854
5918 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G041480.1

Sequence Viewer

Length: 4812 bp
ATGACTGCCAAATTAGATCTTCCTTCTTCTTCTTCTTCTTCTTGTCCTCCTCCTTGTAAATTGACATACGATGTGTTCCTCAGTTTCAGAGGTACAGATACCCGCACAAATTTCACAGACCATCTTTATGCCGCTCTCAAACAGAAAGGCATATACACATTTAGAGATGATGAAGAACTTAACAGAGGGGAAACAATTGCTCCAAGTCTCTTGAAAGCAATTGAAGAGTCCAGATATGTAATTGTGGTGTTTTCTCAAAATTACGCCGATTCTGCGTGGTGTTTGGATGAGCTTGCAAAAGTCGCTGAATGCAGGAGAGCCATGGGGCAAACAGTCCTCCCTGTGTTCTATGATGTTAAGCCGTTTGAGGTACGAAGGCAAAAAGGTGATCACTTTGAGAAAGCATTTCAGAAGCATCGAGAGCGGTGTAAAGAAGAACCAGGCAAAGTAGAGAGGTGGAAAGATGCTCTTGCTGAAGTAGGCAATATCTCTGGATGGGATCTGAAAGATCGGTACCAATCCCAAGTCATTCAAGAAATTGTTGAAAAGATTTTCACCGAGTTAAATCAAATATTCACAATATATGAGGATTTAGTTGGGATGGATTCTCACCTGAATGAAATGCTTTCGTACTTAGACATTGGGTGTCCTGATGTTCGTATCATAGGGATTTGTGGTATGGGTGGTATCGGTAAGACAACTATTGCACAAGTGGTTTTTGAAAGGGTACAGGCCCAGTTTGAAGGTTGCAGCTTTCTTGAGAATGTTAGAGAGGTAATTGAAAAGCAAGGTGCGGTTCATTTACAAGAGCAACTTCTTTCGAATTTGCTGGGAGGTAGTGTAAAATATAATGTACAGAACACTAGAATGGGAAAAAATATAATGAGGCAGAGGCTACATTCTAAAATGGTTCTTATCATTCTTGATGATGTGGACCAGGAAGAACAATTGGAAGCATTGTGTGATAGGGAATCGTTTGGTCCAGGAAGTAGAATCATCATAACTTGTAGAGATGAACATTTGCTCAGTGCAGTTGAAGGAGACAAAGTATATAAGGTGAATCAATTAAGTGATGCTGAAGCTCTTCAGCTTTTCAGTATGAAAGCCTTCAAGAAAGACCAGCAGGTTGGGAAAGATTTTCTCATGCTATCCAAGAAATTTTTGAAATATGCTGATGGCCTTCCACTAGCTATTAAAGTTTTGGGCTCCTCTGTTCGTGGTAGAAGAGTAGAGTTATGGTTAAGTTCATTGGATAGACTAAAAAACAATCCTCAAAAAGGGATTATTGATGTTCTTAAAGTTAGTTTTGATGGACTAGAAGAAACCGAAAAAAAAATGTTTTTGGACATCGCATGTTTCTTTAAAGGAGTCTATAGAGAGCATATAACAAGAATACTACTAGGTAGTGACCACTGTCCGGACATTGATATAGAAGTTCTCATAGAGAAATCTTTGGTAACTCCATTTGGAAGAAGATTGGGGATGCATGACTTGGTACAACAAATGGGTTGGGAAATTGTACGTCAAGAATGCCGTGAAGAGCCTGGAAAACGTAGCAGGTGGTGGCTTCCCGATGAAATCATCCATGTACTTGGTCGGAGTAAGGCCACGAGTGCAGTTCAAAGCATAATCCTCGAATGTCCAACAAAAGACATTGTTGTTCACTCAATTGATAATGCTTTCTCAAATATGGATAGATTGCGATTGCTCAAGATTCGGAATGTGAAGCTTTCTGGAAACATCAGATATCTTTCTAATGAGTTACAGTATCTTGAATGGCATGATTGTCCTTTGGATTCTTTCCCGTCAGACTTCCAACCTGATAAACTTGTGGAACTTAACATGCATTATAGCCGAATCAAACAACTATGGAGGGGAAAAAAAGGATGGAGCATGCTACGGCTTATTAATCTGAGTGGTTCACAATACTTGATGTCGACTCCAGACTTCACTGGGACTCCTGATCTTGAAGAGCTGGTGCTTGAAAATTGCACAAGCTTGGTGGTGGTTCACCCGTCTCTTGGATTTCTGAAGAAACTCTTCTTTTTGAATATGAAAAATTGCAAGTCTATTGAGACCCTTCCACCTTTCACTAGCTTGGAATCCCTTGAAAACTTATGGCTTTCACTCTGTTCAGGACTGAAGAAGCTTCCAGAAATTGAAGGAAATATGAAAAGCTTGCTGGTGCTTCATTTGGACGGGACCAGCATTGAGGAATTGCCTCCATCAATTGAACGCTTGACTGGTCTGACTATGTTGAATCTAACGGACTGTAAAAACCTTTTGCATCTTCCCAAAACCATTGGGTGCTTGACATCTTTGAAAAGTCTCCATCTAACAGGTTGCTCCAAAATTGATGAGATACCAGGGAATCTGAATGGTATGAAATCTCTGGAGATGCTTACAATTGGTGGAACTTCCGTAAGGGAGTTATCTTTCATTGTAGGCATGAAGAATCTACAGTATCTATCCTGCCGAGGATGTAAATGTCTAGTTTCAGAATCATTTAAAGAGATGGCTTCGTTATCGAATTTGATAGGGCTAGACTTGAGTTATTGCAATCTGATGGATGGAGCAATTCTCAATGATTTTAGCAGCCTAATCTCCTTAAGAAGGTTAGATTTAAGTGGTAATTGCTTTGCGCGATTACCTGAAAGTATCTCTCAACTCTCAAAGCTCGAGACTCTCAGCTTGAGAAACTGCAGACAGCTTCAATTGCTGCCCAAGAAGCTTCCGTTAAGTCTTGGAGGCGTGAATGCACAAGATTGTACTTCACTGACGGATTACCCAAATCAAATCAAAACATTGAATTCATTGGAGTCAGGCGTGACCATTGTCAATTCCCTAAATTCTTCAGCACTTGGAGTCTCACAAAGTCCCACATGCTTTATTTCAAGAACAAGAAATGAGTGGGGATCTGCGGACCTGCGTGTCACATATGAGGATATGGAGGGGAAACAAGTTTATCATTTGCCAGTGACATCTCTAAAACAAATGCCTTTCATTTCAGAATTCAGTGACGTACGTCCTAGAGATGAAATTCCAGAGTGGTTCAGCACTTTATCTACCAGGAATCCCATATCAATCTCGATACCTCAAAATCTGGCCAATGATAACAAGTGGAAGGGATTTGCCGCGTGTGCTATATTCTCAGTCAATGGGCATACTGCTCTCTCCGTTATTAAACCGGATCCTGATTTTTCTAATTACTCGTATCAAATCACATTGGAAACCGATGTTGTGGTGGAACCATATGTGTTGGAGGGAGAGACCTTCTCCTCCCCCCATCTGGAATTAGGATCAAGTTCTCATCTGCTTGTGATTTTCTATATATCTAGTTTGAAGTTTCCACAGCGGGGGTTAAATCAATCAACTATGGTGAGAGCTATATTTGAAACCACCAATCCATCCATGGTGGTCCAGAAATGTGGAATTCGTCTAGTCTATGAGCAAGATGCTGGATGGCATTCCAGAATAGACGAGGGGGGTGATTTGATTTGTCTGAATGAAGTTGAGGACGCCGCCTCTGTATTGGAGTCTGGCTGGGTTAGTGAATCTGATCAATGGGAGAAATTCATTCCACCATTGGAAGAAGAATCCACACTTATTCTGAGAAAGAACTTTGAGTCTGTTCTTCCAAGATATCTTGAGGCACTAAACCGTTATTCTACATGGTACAAATTTAACCTCAGAGGAAGACCAGGATGGTTTCATGAGTCATTTACCTTTGATACGAGGTCAAATTTTTGCAGTGTATTAATCGAGCTGCCTCAAAATCTACACAAGAGCAAGAAGTGGATGGGATTTGCAATATGCGCATCACTTGTAGAGGAAGTGGGGCAGACAGAGAAAAATGACAACTATCGGGTACATGTTGAACTAAGAACTGGGGATAAAGGCAGGGTTGTGGCCCAAGTACTTAAGCACGTCAGGCCATTGTCAGAAGAACATCATCAACTATTGATTATTTACATACCGCGGGCAGAGATTCCCGAAGTGTTGCTCACTCAGACAATAACATCTGCAATGTACTTTTCCATCAGTGATAGCGCTCATGTAAAGGTTCAAAAATGTGGGTTCCGTATTGTGTACCAGGAAGATATGCAAGGATTTGCTGATACAATTTTTCGGTGCATGCAAAAGGAAGATTCTCTTAAATCTTCTCTTAAATCTCACAATAAGTTTATGGTAGAAGAATGGATAAAGTTGATACGATTGCAAGGTGCCCATTTAGAGAGAATGACAGAACGGGATTCCAGAACCCCACGGGAAAAATACTTTGAATTGCTCTCAAGAAAATATAGAAATTGGGATTGGTTTGGCCCACGTTCTGTTTCTTGTCACTTCGAGGGAGCTAAAATTTTCAAGTGGAAAAGGTTCATGCCTTTCATCAACCAAGGCAACTCTGCAGAAATCCAACTGCCTCTGAATTTGTTCGATGATGATAATTGGTTGGGGTTTGCCGCATGTTGTCAATTGTCCCACAACCAACATCCAAACATTAGTCTTGGCAGTACTGCTCCTGATTCAGAGGATGTTATGTTTGCTTGCCGTCTGGACTCTGATGTTGCTTGGCGGGGCTTATTTGATGACACCATGCATTTAAGTTTAAAGATTCAAGAGCCATCGGTGGTCAATACGACTTTGTTCTTTTATACACGGCGCACTAGGCTTAGTAAGGTTTGGAAGCAATGTAAATTGGCCAGGATAACTATGTTCTCTAGCCCATGCTTAGCGGTGCATAGTTGTGCCCTTCGTCTACTATTTAAGGAGGACGTTCTCTATTTAAGGAGGACGTTGAACATCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1603

Amino Acids

182.78

Weight (kDa)

6.42

Isoelectric Point (pI)

44.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 23 - 194 1e-54 TIR domain
TIR_2 PF13676 25 - 122 4.9e-16 TIR domain
NB-ARC PF00931 205 - 374 4.9e-23 NB-ARC domain
WHD_ROQ1 PF23282 440 - 509 3.9e-16 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 671 - 780 1.6e-09 Leucine-rich repeat region
LRR_13 PF23286 700 - 787 6.7e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 848 - 941 7.9e-09 Leucine-rich repeat region
C-JID PF20160 1010 - 1153 4.7e-10 C-JID domain
C-JID PF20160 1243 - 1368 4.3e-08 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1548
AasI GACNNNNNNGTC 1 cut(s) 2930
Acc16I TGCGCA 1 cut(s) 3817
Acc36I ACCTGC 3 cut(s) 1114, 1548, 2934
Acc65I GGTACC 1 cut(s) 513
AccB1I GGYRCC 2 cut(s) 513, 4223
AccBSI CCGCTC 2 cut(s) 134, 424
AccI GTMKAC 2 cut(s) 1937, 4759
AccII CGCG 3 cut(s) 2644, 3137, 3979
AccIII TCCGGA 1 cut(s) 1417
AclWI GGATC 5 cut(s) 507, 2923, 3185, 3198, 3307
AcoI YGGCCR 2 cut(s) 3105, 4701
AcuI CTGAAG 6 cut(s) 495, 1070, 1098, 2051, 2162, 2838
AcyI GRCGYC 1 cut(s) 3519
AfeI AGCGCT 1 cut(s) 4051
AfiI CCNNNNNNNGG 6 cut(s) 1277, 1417, 2021, 2613, 3289, 3356
AflII CTTAAG 2 cut(s) 2608, 3920
AflIII ACRYGT 1 cut(s) 3871
AjiI CACGTC 1 cut(s) 3928
AjnI CCWGG 9 cut(s) 439, 936, 982, 1543, 2365, 3068, 3700, 4092, 4703
AjuI GAANNNNNNNTTGG 2 cut(s) 4297, 4329
Alw26I GTCTC 8 cut(s) 212, 1035, 2022, 2069, 2333, 2675, 2872, 3263
AlwI GGATC 5 cut(s) 507, 2923, 3185, 3198, 3307
Ama87I CYCGRG 1 cut(s) 2678
Aor13HI TCCGGA 1 cut(s) 1417
Aor51HI AGCGCT 1 cut(s) 4051
AoxI GGCC 8 cut(s) 732, 1177, 1605, 3105, 3909, 3932, 4321, 4701
ApeKI GCWGC 4 cut(s) 750, 2595, 2719, 3766
AseI ATTAAT 2 cut(s) 1908, 3758
Asp700I GAANNNNTTC 5 cut(s) 1083, 1106, 2039, 4376, 4433
Asp718I GGTACC 1 cut(s) 513
AspLEI GCGC 4 cut(s) 2644, 3818, 4052, 4665
AspS9I GGNCC 8 cut(s) 733, 934, 980, 2202, 2923, 3418, 3910, 4322
AsuHPI GGTGA 7 cut(s) 398, 547, 602, 1069, 2003, 3391, 3500
AsuII TTCGAA 1 cut(s) 821
AvaI CYCGRG 1 cut(s) 2678
AvaII GGWCC 5 cut(s) 934, 980, 2202, 2923, 3418
BaeGI GKGCMC 2 cut(s) 4228, 4753
BalI TGGCCA 2 cut(s) 3107, 4703
BamHI GGATCC 1 cut(s) 3190
BanI GGYRCC 2 cut(s) 513, 4223
BanII GRGCYC 1 cut(s) 1208
BauI CACGAG 1 cut(s) 1609
BbsI GAAGAC 1 cut(s) 3703
BbvI GCAGC 4 cut(s) 762, 2607, 2706, 3753
BceAI ACGGC 5 cut(s) 346, 1518, 1916, 4536, 4676
BcgI CGANNNNNNTGC 2 cut(s) 1615, 1649
BciT130I CCWGG 9 cut(s) 441, 938, 984, 1545, 2367, 3070, 3702, 4094, 4705
BclI TGATCA 2 cut(s) 388, 3559
BcoDI GTCTC 8 cut(s) 212, 1035, 2022, 2069, 2333, 2675, 2872, 3263
BfmI CTRYAG 4 cut(s) 1372, 2459, 2701, 4407
BfoI RGCGCY 1 cut(s) 4053
BfrI CTTAAG 2 cut(s) 2608, 3920
BfuAI ACCTGC 3 cut(s) 1114, 1548, 2934
BglII AGATCT 1 cut(s) 16
BisI GCNGC 8 cut(s) 132, 751, 2596, 2720, 3135, 3522, 3767, 4464
BlpI GCTNAGC 1 cut(s) 4732
BlsI GCNGC 8 cut(s) 133, 752, 2597, 2721, 3136, 3523, 3768, 4465
BmcAI AGTACT 2 cut(s) 3918, 4516
Bme1390I CCNGG 9 cut(s) 441, 938, 984, 1545, 2367, 3070, 3702, 4094, 4705
Bme18I GGWCC 5 cut(s) 934, 980, 2202, 2923, 3418
BmeT110I CYCGRG 1 cut(s) 2678
BmgBI CACGTC 1 cut(s) 3928
BmgT120I GGNCC 8 cut(s) 733, 934, 980, 2202, 2923, 3418, 3910, 4322
BmiI GGNNCC 7 cut(s) 515, 1207, 2203, 3192, 3249, 4079, 4225
BmrFI CCNGG 9 cut(s) 441, 938, 984, 1545, 2367, 3070, 3702, 4094, 4705
BmrI ACTGGG 3 cut(s) 730, 1962, 3897
BmsI GCATC 8 cut(s) 424, 454, 1063, 1473, 2296, 2388, 3445, 3827
BmuI ACTGGG 3 cut(s) 730, 1962, 3897
BoxI GACNNNNGTC 3 cut(s) 1413, 2834, 3024
BpiI GAAGAC 1 cut(s) 3703
BplI GAGNNNNNCTC 4 cut(s) 2565, 2597, 3260, 3292
BpmI CTGGAG 2 cut(s) 1926, 2414
Bpu1102I GCTNAGC 1 cut(s) 4732
Bpu14I TTCGAA 1 cut(s) 821
BpuEI CTTGAG 6 cut(s) 779, 1694, 2569, 2713, 3668, 4276
BsaHI GRCGYC 1 cut(s) 3519
BsaI GGTCTC 2 cut(s) 2069, 3263
BsaJI CCNNGG 7 cut(s) 321, 2366, 2476, 3411, 3977, 4265, 4396
BsaWI WCCGGW 2 cut(s) 1417, 3187
BsaXI ACNNNNNCTCC 8 cut(s) 184, 214, 825, 855, 1032, 1062, 1942, 1972
Bsc4I CCNNNNNNNGG 6 cut(s) 1277, 1417, 2021, 2613, 3289, 3356
Bse1I ACTGG 5 cut(s) 736, 1957, 2248, 2975, 3892
Bse3DI GCAATG 2 cut(s) 4032, 4697
BseAI TCCGGA 1 cut(s) 1417
BseBI CCWGG 9 cut(s) 441, 938, 984, 1545, 2367, 3070, 3702, 4094, 4705
BseDI CCNNGG 7 cut(s) 321, 2366, 2476, 3411, 3977, 4265, 4396
BseLI CCNNNNNNNGG 6 cut(s) 1277, 1417, 2021, 2613, 3289, 3356
BseMI GCAATG 2 cut(s) 4032, 4697
BseMII CTCAG 8 cut(s) 94, 1039, 1904, 2701, 3165, 3602, 3703, 4022
BseNI ACTGG 5 cut(s) 736, 1957, 2248, 2975, 3892
BseRI GAGGAG 3 cut(s) 39, 1198, 3268
BseSI GKGCMC 2 cut(s) 4228, 4753
BseXI GCAGC 4 cut(s) 762, 2607, 2706, 3753
BseYI CCCAGC 2 cut(s) 829, 3543
BsgI GTGCAG 2 cut(s) 1050, 1635
Bsh1236I CGCG 3 cut(s) 2644, 3137, 3979
BshFI GGCC 8 cut(s) 734, 1179, 1607, 3107, 3911, 3934, 4323, 4703
BshNI GGYRCC 2 cut(s) 513, 4223
BsiHKCI CYCGRG 1 cut(s) 2678
BsiSI CCGG 2 cut(s) 1418, 3188
BsiWI CGTACG 1 cut(s) 3022
BslFI GGGAC 4 cut(s) 1969, 2215, 2862, 4465
BslI CCNNNNNNNGG 6 cut(s) 1277, 1417, 2021, 2613, 3289, 3356
BsmAI GTCTC 8 cut(s) 212, 1035, 2022, 2069, 2333, 2675, 2872, 3263
BsmBI CGTCTC 1 cut(s) 2022
BsmFI GGGAC 4 cut(s) 1969, 2215, 2862, 4465
BsmI GAATGC 4 cut(s) 314, 1535, 2761, 3466
BsnI GGCC 8 cut(s) 734, 1179, 1607, 3107, 3911, 3934, 4323, 4703
Bso31I GGTCTC 2 cut(s) 2069, 3263
BsoBI CYCGRG 1 cut(s) 2678
Bsp119I TTCGAA 1 cut(s) 821
Bsp1286I GDGCHC 3 cut(s) 1208, 4228, 4753
Bsp13I TCCGGA 1 cut(s) 1417
Bsp1407I TGTACA 1 cut(s) 853
Bsp143I GATC 9 cut(s) 16, 388, 499, 508, 1963, 2915, 3190, 3299, 3559
Bsp1720I GCTNAGC 1 cut(s) 4732
Bsp19I CCATGG 2 cut(s) 321, 3411
BspANI GGCC 8 cut(s) 734, 1179, 1607, 3107, 3911, 3934, 4323, 4703
BspCNI CTCAG 8 cut(s) 93, 1038, 1905, 2700, 3164, 3603, 3702, 4021
BspEI TCCGGA 1 cut(s) 1417
BspFNI CGCG 3 cut(s) 2644, 3137, 3979
BspHI TCATGA 1 cut(s) 3712
BspLI GGNNCC 7 cut(s) 515, 1207, 2203, 3192, 3249, 4079, 4225
BspMAI CTGCAG 2 cut(s) 2705, 4411
BspMI ACCTGC 3 cut(s) 1114, 1548, 2934
BspPI GGATC 5 cut(s) 507, 2923, 3185, 3198, 3307
BspQI GCTCTTC 3 cut(s) 1089, 1533, 1965
BspT104I TTCGAA 1 cut(s) 821
BspT107I GGYRCC 2 cut(s) 513, 4223
BspTI CTTAAG 2 cut(s) 2608, 3920
BspTNI GGTCTC 2 cut(s) 2069, 3263
BsrBI CCGCTC 2 cut(s) 134, 424
BsrDI GCAATG 2 cut(s) 4032, 4697
BsrGI TGTACA 1 cut(s) 853
BsrI ACTGG 5 cut(s) 736, 1957, 2248, 2975, 3892
BssECI CCNNGG 7 cut(s) 321, 2366, 2476, 3411, 3977, 4265, 4396
BssMI GATC 9 cut(s) 16, 388, 499, 508, 1963, 2915, 3190, 3299, 3559
BssNI GRCGYC 1 cut(s) 3519
BssSI CACGAG 1 cut(s) 1609
BssT1I CCWWGG 3 cut(s) 321, 3411, 4396
Bst2BI CACGAG 1 cut(s) 1609
Bst2UI CCWGG 9 cut(s) 441, 938, 984, 1545, 2367, 3070, 3702, 4094, 4705
Bst4CI ACNGT 6 cut(s) 334, 1415, 1767, 2273, 2463, 3662
Bst6I CTCTTC 6 cut(s) 219, 1089, 1219, 1533, 1965, 2045
BstACI GRCGYC 1 cut(s) 3519
BstAFI CTTAAG 2 cut(s) 2608, 3920
BstAUI TGTACA 1 cut(s) 853
BstBI TTCGAA 1 cut(s) 821
BstC8I GCNNGC 6 cut(s) 294, 1895, 2180, 3981, 4136, 4549
BstDSI CCRYGG 4 cut(s) 321, 3411, 3977, 4265
BstENI CCTNNNNNAGG 2 cut(s) 1275, 2611
BstFNI CGCG 3 cut(s) 2644, 3137, 3979
BstH2I RGCGCY 1 cut(s) 4053
BstHHI GCGC 4 cut(s) 2644, 3818, 4052, 4665
BstKTI GATC 9 cut(s) 19, 391, 502, 511, 1966, 2918, 3193, 3302, 3562
BstMAI GTCTC 8 cut(s) 212, 1035, 2022, 2069, 2333, 2675, 2872, 3263
BstMBI GATC 9 cut(s) 16, 388, 499, 508, 1963, 2915, 3190, 3299, 3559
BstNI CCWGG 9 cut(s) 441, 938, 984, 1545, 2367, 3070, 3702, 4094, 4705
BstNSI RCATGY 7 cut(s) 1356, 1846, 1897, 2886, 3875, 4138, 4470
BstPAI GACNNNNGTC 3 cut(s) 1413, 2834, 3024
BstSCI CCNGG 9 cut(s) 439, 936, 982, 1543, 2365, 3068, 3700, 4092, 4703
BstSFI CTRYAG 4 cut(s) 1372, 2459, 2701, 4407
BstSLI GKGCMC 2 cut(s) 4228, 4753
BstUI CGCG 3 cut(s) 2644, 3137, 3979
BstV1I GCAGC 4 cut(s) 762, 2607, 2706, 3753
BstV2I GAAGAC 1 cut(s) 3703
BstX2I RGATCY 4 cut(s) 16, 499, 2915, 3190
BstXI CCANNNNNNTGG 3 cut(s) 1127, 1592, 3428
BstYI RGATCY 4 cut(s) 16, 499, 2915, 3190
BsuRI GGCC 8 cut(s) 734, 1179, 1607, 3107, 3911, 3934, 4323, 4703
BtgI CCRYGG 4 cut(s) 321, 3411, 3977, 4265
BtgZI GCGATG 1 cut(s) 1333
BtrI CACGTC 1 cut(s) 3928
BtsI GCAGTG 1 cut(s) 3757
BtsIMutI CAGTG 8 cut(s) 1033, 1411, 1950, 2774, 2982, 3022, 3757, 4048
BveI ACCTGC 3 cut(s) 1114, 1548, 2934
Cac8I GCNNGC 6 cut(s) 294, 1895, 2180, 3981, 4136, 4549
CciI TCATGA 1 cut(s) 3712
CfoI GCGC 4 cut(s) 2644, 3818, 4052, 4665
Cfr13I GGNCC 8 cut(s) 733, 934, 980, 2202, 2923, 3418, 3910, 4322
Cfr42I CCGCGG 1 cut(s) 3980
CseI GACGC 1 cut(s) 3527
CspCI CAANNNNNGTGG 2 cut(s) 1983, 2018
DpnI GATC 9 cut(s) 18, 390, 501, 510, 1965, 2917, 3192, 3301, 3561
DpnII GATC 9 cut(s) 16, 388, 499, 508, 1963, 2915, 3190, 3299, 3559
DraI TTTAAA 3 cut(s) 1363, 2509, 4611
DrdI GACNNNNNNGTC 1 cut(s) 2930
DseDI GACNNNNNNGTC 1 cut(s) 2930
EaeI YGGCCR 2 cut(s) 3105, 4701
Eam1104I CTCTTC 6 cut(s) 219, 1089, 1219, 1533, 1965, 2045
EarI CTCTTC 6 cut(s) 219, 1089, 1219, 1533, 1965, 2045
Eco130I CCWWGG 3 cut(s) 321, 3411, 4396
Eco24I GRGCYC 1 cut(s) 1208
Eco31I GGTCTC 2 cut(s) 2069, 3263
Eco32I GATATC 2 cut(s) 1748, 3644
Eco47I GGWCC 5 cut(s) 934, 980, 2202, 2923, 3418
Eco47III AGCGCT 1 cut(s) 4051
Eco57I CTGAAG 6 cut(s) 495, 1070, 1098, 2051, 2162, 2838
Eco88I CYCGRG 1 cut(s) 2678
EcoNI CCTNNNNNAGG 2 cut(s) 1275, 2611
EcoRI GAATTC 3 cut(s) 2809, 3011, 3432
EcoRII CCWGG 9 cut(s) 439, 936, 982, 1543, 2365, 3068, 3700, 4092, 4703
EcoRV GATATC 2 cut(s) 1748, 3644
EcoT14I CCWWGG 3 cut(s) 321, 3411, 4396
EcoT22I ATGCAT 3 cut(s) 1488, 1848, 4602
EcoT38I GRGCYC 1 cut(s) 1208
ErhI CCWWGG 3 cut(s) 321, 3411, 4396
Esp3I CGTCTC 1 cut(s) 2022
FalI AAGNNNNNCTT 8 cut(s) 453, 485, 798, 830, 2024, 2056, 4137, 4169
FaqI GGGAC 4 cut(s) 1969, 2215, 2862, 4465
FauI CCCGC 4 cut(s) 110, 3348, 3972, 4569
FauNDI CATATG 2 cut(s) 2938, 3253
FbaI TGATCA 2 cut(s) 388, 3559
FblI GTMKAC 2 cut(s) 1937, 4759
Fnu4HI GCNGC 8 cut(s) 132, 751, 2596, 2720, 3135, 3522, 3767, 4464
FriOI GRGCYC 1 cut(s) 1208
Fsp4HI GCNGC 8 cut(s) 132, 751, 2596, 2720, 3135, 3522, 3767, 4464
FspAI RTGCGCAY 1 cut(s) 3817
FspI TGCGCA 1 cut(s) 3817
GlaI GCGC 4 cut(s) 2643, 3817, 4051, 4664
GluI GCNGC 8 cut(s) 132, 751, 2596, 2720, 3135, 3522, 3767, 4464
GsaI CCCAGC 2 cut(s) 833, 3547
GsuI CTGGAG 2 cut(s) 1926, 2414
HaeII RGCGCY 1 cut(s) 4053
HaeIII GGCC 8 cut(s) 734, 1179, 1607, 3107, 3911, 3934, 4323, 4703
HapII CCGG 2 cut(s) 1418, 3188
HgaI GACGC 1 cut(s) 3527
HhaI GCGC 4 cut(s) 2644, 3818, 4052, 4665
Hin1I GRCGYC 1 cut(s) 3519
Hin6I GCGC 4 cut(s) 2642, 3816, 4050, 4663
HinP1I GCGC 4 cut(s) 2642, 3816, 4050, 4663
HincII GTYRAC 1 cut(s) 1938
HindII GTYRAC 1 cut(s) 1938
HindIII AAGCTT 5 cut(s) 1727, 1996, 2147, 2176, 2729
HpaII CCGG 2 cut(s) 1418, 3188
HphI GGTGA 7 cut(s) 398, 547, 602, 1069, 2003, 3391, 3500
Hpy166II GTNNAC 7 cut(s) 934, 1663, 1922, 1938, 2011, 4090, 4760
Hpy8I GTNNAC 7 cut(s) 934, 1663, 1922, 1938, 2011, 4090, 4760
HpyCH4III ACNGT 6 cut(s) 334, 1415, 1767, 2273, 2463, 3662
HpyCH4IV ACGT 8 cut(s) 1522, 1552, 3021, 3025, 3927, 4327, 4776, 4796
HpySE526I ACGT 8 cut(s) 1522, 1552, 3021, 3025, 3927, 4327, 4776, 4796
Hsp92I GRCGYC 1 cut(s) 3519
HspAI GCGC 4 cut(s) 2642, 3816, 4050, 4663
Kpn2I TCCGGA 1 cut(s) 1417
KpnI GGTACC 1 cut(s) 517
Ksp22I TGATCA 2 cut(s) 388, 3559
KspI CCGCGG 1 cut(s) 3980
Kzo9I GATC 9 cut(s) 16, 388, 499, 508, 1963, 2915, 3190, 3299, 3559
LguI GCTCTTC 3 cut(s) 1089, 1533, 1965
LmnI GCTCC 7 cut(s) 205, 1211, 1890, 2351, 2573, 4352, 4525
Lsp1109I GCAGC 4 cut(s) 762, 2607, 2706, 3753
LweI GCATC 8 cut(s) 424, 454, 1063, 1473, 2296, 2388, 3445, 3827
MaeII ACGT 8 cut(s) 1522, 1552, 3021, 3025, 3927, 4327, 4776, 4796
MaeIII GTNAC 8 cut(s) 1406, 1456, 1761, 2827, 2932, 2977, 3017, 4340
MalI GATC 9 cut(s) 18, 390, 501, 510, 1965, 2917, 3192, 3301, 3561
MbiI CCGCTC 2 cut(s) 134, 424
MboI GATC 9 cut(s) 16, 388, 499, 508, 1963, 2915, 3190, 3299, 3559
MfeI CAATTG 8 cut(s) 195, 219, 947, 1668, 2229, 2406, 2714, 4475
MflI RGATCY 4 cut(s) 16, 499, 2915, 3190
MhlI GDGCHC 3 cut(s) 1208, 4228, 4753
MlsI TGGCCA 2 cut(s) 3107, 4703
MluNI TGGCCA 2 cut(s) 3107, 4703
MmeI TCCRAC 5 cut(s) 1577, 1667, 1840, 3240, 4441
Mox20I TGGCCA 2 cut(s) 3107, 4703
Mph1103I ATGCAT 3 cut(s) 1488, 1848, 4602
MroI TCCGGA 1 cut(s) 1417
MroXI GAANNNNTTC 5 cut(s) 1083, 1106, 2039, 4376, 4433
MscI TGGCCA 2 cut(s) 3107, 4703
MslI CAYNNNNRTG 4 cut(s) 126, 615, 866, 4746
Msp20I TGGCCA 2 cut(s) 3107, 4703
MspA1I CMGCKG 2 cut(s) 3355, 3979
MspCI CTTAAG 2 cut(s) 2608, 3920
MspI CCGG 2 cut(s) 1418, 3188
MspR9I CCNGG 9 cut(s) 441, 938, 984, 1545, 2367, 3070, 3702, 4094, 4705
MunI CAATTG 8 cut(s) 195, 219, 947, 1668, 2229, 2406, 2714, 4475
Mva1269I GAATGC 4 cut(s) 314, 1535, 2761, 3466
MvaI CCWGG 9 cut(s) 441, 938, 984, 1545, 2367, 3070, 3702, 4094, 4705
MvnI CGCG 3 cut(s) 2644, 3137, 3979
NcoI CCATGG 2 cut(s) 321, 3411
NdeI CATATG 2 cut(s) 2938, 3253
NdeII GATC 9 cut(s) 16, 388, 499, 508, 1963, 2915, 3190, 3299, 3559
NlaIV GGNNCC 7 cut(s) 515, 1207, 2203, 3192, 3249, 4079, 4225
NmeAIII GCCGAG 1 cut(s) 2501
NmuCI GTSAC 6 cut(s) 1406, 2827, 2932, 2977, 3017, 4340
NsbI TGCGCA 1 cut(s) 3817
NsiI ATGCAT 3 cut(s) 1488, 1848, 4602
NspI RCATGY 7 cut(s) 1356, 1846, 1897, 2886, 3875, 4138, 4470
NspV TTCGAA 1 cut(s) 821
PaeI GCATGC 2 cut(s) 1897, 4138
PaeR7I CTCGAG 1 cut(s) 2678
PagI TCATGA 1 cut(s) 3712
PaqCI CACCTGC 1 cut(s) 1548
PciI ACATGT 1 cut(s) 3871
PciSI GCTCTTC 3 cut(s) 1089, 1533, 1965
PctI GAATGC 4 cut(s) 314, 1535, 2761, 3466
PdmI GAANNNNTTC 5 cut(s) 1083, 1106, 2039, 4376, 4433
Pfl23II CGTACG 1 cut(s) 3022
PfoI TCCNGGA 1 cut(s) 982
PkrI GCNGC 8 cut(s) 133, 752, 2597, 2721, 3136, 3523, 3768, 4465
PscI ACATGT 1 cut(s) 3871
PshAI GACNNNNGTC 3 cut(s) 1413, 2834, 3024
PshBI ATTAAT 2 cut(s) 1908, 3758
Psp6I CCWGG 9 cut(s) 439, 936, 982, 1543, 2365, 3068, 3700, 4092, 4703
PspFI CCCAGC 2 cut(s) 829, 3543
PspGI CCWGG 9 cut(s) 439, 936, 982, 1543, 2365, 3068, 3700, 4092, 4703
PspLI CGTACG 1 cut(s) 3022
PspN4I GGNNCC 7 cut(s) 515, 1207, 2203, 3192, 3249, 4079, 4225
PspPI GGNCC 8 cut(s) 733, 934, 980, 2202, 2923, 3418, 3910, 4322
PstI CTGCAG 2 cut(s) 2705, 4411
PsuI RGATCY 4 cut(s) 16, 499, 2915, 3190
RseI CAYNNNNRTG 4 cut(s) 126, 615, 866, 4746
SacII CCGCGG 1 cut(s) 3980
SalI GTCGAC 1 cut(s) 1936
SapI GCTCTTC 3 cut(s) 1089, 1533, 1965
SatI GCNGC 8 cut(s) 132, 751, 2596, 2720, 3135, 3522, 3767, 4464
Sau3AI GATC 9 cut(s) 16, 388, 499, 508, 1963, 2915, 3190, 3299, 3559
Sau96I GGNCC 8 cut(s) 733, 934, 980, 2202, 2923, 3418, 3910, 4322
ScaI AGTACT 2 cut(s) 3918, 4516
ScrFI CCNGG 9 cut(s) 441, 938, 984, 1545, 2367, 3070, 3702, 4094, 4705
SduI GDGCHC 3 cut(s) 1208, 4228, 4753
SfaNI GCATC 8 cut(s) 424, 454, 1063, 1473, 2296, 2388, 3445, 3827
SfcI CTRYAG 4 cut(s) 1372, 2459, 2701, 4407
Sfr274I CTCGAG 1 cut(s) 2678
Sfr303I CCGCGG 1 cut(s) 3980
SfuI TTCGAA 1 cut(s) 821
SgrBI CCGCGG 1 cut(s) 3980
SinI GGWCC 5 cut(s) 934, 980, 2202, 2923, 3418
SlaI CTCGAG 1 cut(s) 2678
SmiMI CAYNNNNRTG 4 cut(s) 126, 615, 866, 4746
SmlI CTYRAG 9 cut(s) 758, 1709, 2548, 2608, 2678, 2692, 3647, 3920, 4291
SmoI CTYRAG 9 cut(s) 758, 1709, 2548, 2608, 2678, 2692, 3647, 3920, 4291
SphI GCATGC 2 cut(s) 1897, 4138
SspI AATATT 1 cut(s) 573
StyD4I CCNGG 9 cut(s) 439, 936, 982, 1543, 2365, 3068, 3700, 4092, 4703
StyI CCWWGG 3 cut(s) 321, 3411, 4396
TaaI ACNGT 6 cut(s) 334, 1415, 1767, 2273, 2463, 3662
TaiI ACGT 8 cut(s) 1525, 1555, 3024, 3028, 3930, 4330, 4779, 4799
TatI WGTACW 6 cut(s) 853, 1588, 2768, 3916, 4029, 4514
TauI GCSGC 4 cut(s) 134, 3137, 3524, 4466
TscAI CASTG 8 cut(s) 1033, 1418, 1957, 2781, 2982, 3022, 3757, 4048
TseFI GTSAC 6 cut(s) 1406, 2827, 2932, 2977, 3017, 4340
TseI GCWGC 4 cut(s) 750, 2595, 2719, 3766
Tsp45I GTSAC 6 cut(s) 1406, 2827, 2932, 2977, 3017, 4340
TspGWI ACGGA 6 cut(s) 2282, 2410, 2724, 2795, 3166, 4070
TspRI CASTG 8 cut(s) 1033, 1418, 1957, 2781, 2982, 3022, 3757, 4048
Vha464I CTTAAG 2 cut(s) 2608, 3920
VpaK11BI GGWCC 5 cut(s) 934, 980, 2202, 2923, 3418
VspI ATTAAT 2 cut(s) 1908, 3758
XagI CCTNNNNNAGG 2 cut(s) 1275, 2611
XceI RCATGY 7 cut(s) 1356, 1846, 1897, 2886, 3875, 4138, 4470
XcmI CCANNNNNNNNNTGG 1 cut(s) 3409
XhoI CTCGAG 1 cut(s) 2678
XmiI GTMKAC 2 cut(s) 1937, 4759
XmnI GAANNNNTTC 5 cut(s) 1083, 1106, 2039, 4376, 4433
ZrmI AGTACT 2 cut(s) 3918, 4516
Zsp2I ATGCAT 3 cut(s) 1488, 1848, 4602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.