FvH4_5g38500

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000129
Physical Location & Seq
Reverse (-)
28453217 .. 28460566
7350 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g38500.t1

Sequence Viewer

Length: 1494 bp
ATGTACCAGGGATGGGGCATGCTGCGATTCATGGATCTGAGAGGTTCACAATACTTGATATCGACTCCAGACTTCAGTCAGGTTCCAGAACTTGAGATCTTGGTGCTTGCAGATTGTAGAAGCTTGGTTGAAGTTCACCCATCTCTTGGATTTCTAAAGAAACTTTTCTTTTTGAATATGAGAAATTGCAAGTCTCTTGAGTGCATTCCACCTTTCACTAGTGAAACTTCCATAGAGGAATTGCCTCCATCAGTTGAAAGGTTGACTGGTCTTACCATGTTAAGCTTAAGAGACTGCAAACACCTCTTGTATCTCCCTGCTACCATTCAATTCTTGACATCCTTGAAAAGTCTGACTTTAATAGGCTGCTCAAACCTTGATAAGATACCTGAGAATCTGAAGTCTGTGGAATGTCTGGAGGAGCTGCTAGTAGATGGTACTGCTATAAAGGAGTTATCCTTCCTTGTTGGTATGAAGAATCTAAGATATCTATCCTGCGAAGGATGTAAAGGTCTTCATCACGCTTCCAATATTGCTGCCCAAGCTTCCATCAAGTTGTCGACATGTGGACGCACGAGGTTGTCCCTCACTGCCGGGAGCCTCAAATCAATTCAAAGTATTGACATCTTTTTTGAATCTGGAGTGACTACTGTCAATTATCTCGATTCTCGTGGAGACTTGGAAAGTTTCAAAGGGTGGAGTCATTCAACAGACGCCCAGGGATCTTGTGACATGAGTTTCGGCGTGAGAGCAGACAAGCATTCGGAGTGGGAAGAAGTTGGTCATTTATCAGTTCCAGTTACTACACTTCTAAAAGACATTGAACTTTCAGATGACTTGTCAAATTCTGGTCATGCCGTTTTTGTTGATAATGAAATTCCCGAGTGGTTCAGCAGTGTAACTACCAATAATCCCATATCAATCCAGATACCTCCAAATCTGACAGATGATAACAAGTGGAAGGGAGTTGCTGCATGTGCTGTTTTCTCATTCAAGGGACGTTCTGCTATCTCCCTTGTTGAACCAGATTCTAAAAGTTCTAGTTACTCCTATCAATGCTCAGTGGAAACTGATATATTCTATATGGAACCATCCATTTTGGACCGGGAAGAACTCCTCAGGGTAGTTAGATCAAGCTCTCATATACTTGCGATTTTCTATCTATCTAGCTGTAAGTTTGGAACGTGGTTAGATAGATCAACGGTGATGGCTGCTAGATTTGGAACAAATAACCCATTTATGGAGGTCCAGAAATGTGGAATTCGTCTAGTTTATGAGCAAGATGCTGGATGGTTAGCCAAACTATTCCCTGGTAGTATTGCCATTTATCTAAATGATGTTCAAGACGCCACAGTAAAACCAGGCTGGCTTAGTCAAGTCGATAAATGTTTGAAATGGGAGAATTTTATTCCACCTCGCGAACAAGTCTCCACAATTCTGCTGAGAAAGAACATTGAGTCTGTTCTTCCAAGATACCTTGAGGTTAATATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

498

Amino Acids

55.57

Weight (kDa)

5.37

Isoelectric Point (pI)

43.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 22 - 213 3e-11 Leucine-rich repeat region
LRR_RPS2 PF23247 29 - 142 1.6e-06 Plant disease resistance protein RPS2-like, leucine-rich repeats
C-JID PF20160 290 - 429 4.8e-10 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 146
AccI GTMKAC 1 cut(s) 560
AccII CGCG 1 cut(s) 1419
AclWI GGATC 2 cut(s) 42, 730
AcsI RAATTY 4 cut(s) 844, 876, 1260, 1402
AcuI CTGAAG 2 cut(s) 58, 419
AcyI GRCGYC 2 cut(s) 714, 1347
AfaI GTAC 2 cut(s) 5, 439
AfiI CCNNNNNNNGG 3 cut(s) 13, 146, 1240
AflII CTTAAG 1 cut(s) 286
AflIII ACRYGT 1 cut(s) 563
AhlI ACTAGT 1 cut(s) 218
AjnI CCWGG 4 cut(s) 6, 717, 1309, 1360
AluBI AGCT 6 cut(s) 123, 285, 424, 545, 1137, 1170
AluI AGCT 6 cut(s) 123, 285, 424, 545, 1137, 1170
Alw26I GTCTC 4 cut(s) 198, 285, 669, 1432
AlwI GGATC 2 cut(s) 42, 730
Ama87I CYCGRG 1 cut(s) 881
ApeKI GCWGC 6 cut(s) 22, 366, 424, 536, 971, 1211
ApoI RAATTY 4 cut(s) 844, 876, 1260, 1402
ArsI GACNNNNNNTTYG 4 cut(s) 614, 646, 722, 754
Asp700I GAANNNNTTC 1 cut(s) 164
AspS9I GGNCC 2 cut(s) 1102, 1246
AsuC2I CCSGG 2 cut(s) 595, 1106
AsuHPI GGTGA 2 cut(s) 128, 1216
AvaI CYCGRG 1 cut(s) 881
AvaII GGWCC 2 cut(s) 1102, 1246
AxyI CCTNAGG 1 cut(s) 1118
BauI CACGAG 2 cut(s) 574, 669
BbsI GAAGAC 1 cut(s) 506
BbvI GCAGC 6 cut(s) 9, 353, 411, 523, 958, 1198
BccI CCATC 8 cut(s) 6, 148, 256, 428, 557, 1099, 1201, 1284
BceAI ACGGC 1 cut(s) 842
BciT130I CCWGG 4 cut(s) 8, 719, 1311, 1362
BcnI CCSGG 2 cut(s) 595, 1106
BcoDI GTCTC 4 cut(s) 198, 285, 669, 1432
BcuI ACTAGT 1 cut(s) 218
BfaI CTAG 6 cut(s) 219, 428, 1041, 1167, 1215, 1268
BfrI CTTAAG 1 cut(s) 286
BglII AGATCT 1 cut(s) 96
BisI GCNGC 6 cut(s) 23, 367, 425, 537, 972, 1212
BlsI GCNGC 6 cut(s) 24, 368, 426, 538, 973, 1213
Bme1390I CCNGG 6 cut(s) 8, 595, 719, 1106, 1311, 1362
Bme18I GGWCC 2 cut(s) 1102, 1246
BmeT110I CYCGRG 1 cut(s) 881
BmgT120I GGNCC 2 cut(s) 1102, 1246
BmiI GGNNCC 3 cut(s) 84, 599, 1089
BmrFI CCNGG 6 cut(s) 8, 595, 719, 1106, 1311, 1362
BmsI GCATC 1 cut(s) 1273
BoxI GACNNNNGTC 2 cut(s) 75, 650
BpiI GAAGAC 1 cut(s) 506
BpmI CTGGAG 3 cut(s) 51, 437, 660
BpuEI CTTGAG 2 cut(s) 113, 218
BpuMI CCSGG 2 cut(s) 595, 1106
BsaBI GATNNNNATC 1 cut(s) 490
BsaHI GRCGYC 2 cut(s) 714, 1347
BsaJI CCNNGG 4 cut(s) 7, 717, 718, 1309
Bsc4I CCNNNNNNNGG 3 cut(s) 13, 146, 1240
Bse1I ACTGG 2 cut(s) 271, 797
Bse21I CCTNAGG 1 cut(s) 1118
Bse8I GATNNNNATC 1 cut(s) 490
BseBI CCWGG 4 cut(s) 8, 719, 1311, 1362
BseDI CCNNGG 4 cut(s) 7, 717, 718, 1309
BseGI GGATG 5 cut(s) 17, 338, 509, 1091, 1295
BseJI GATNNNNATC 1 cut(s) 490
BseLI CCNNNNNNNGG 3 cut(s) 13, 146, 1240
BseMII CTCAG 5 cut(s) 29, 381, 1074, 1132, 1433
BseNI ACTGG 2 cut(s) 271, 797
BseRI GAGGAG 2 cut(s) 434, 1106
BseXI GCAGC 6 cut(s) 9, 353, 411, 523, 958, 1198
Bsh1236I CGCG 1 cut(s) 1419
BsiHKCI CYCGRG 1 cut(s) 881
BsiSI CCGG 2 cut(s) 594, 1105
BslFI GGGAC 2 cut(s) 568, 1011
BslI CCNNNNNNNGG 3 cut(s) 13, 146, 1240
BsmAI GTCTC 4 cut(s) 198, 285, 669, 1432
BsmFI GGGAC 2 cut(s) 568, 1011
BsmI GAATGC 2 cut(s) 204, 760
BsoBI CYCGRG 1 cut(s) 881
Bsp143I GATC 5 cut(s) 34, 96, 722, 1130, 1196
Bsp68I TCGCGA 1 cut(s) 1419
BspCNI CTCAG 5 cut(s) 30, 382, 1073, 1131, 1434
BspFNI CGCG 1 cut(s) 1419
BspLI GGNNCC 3 cut(s) 84, 599, 1089
BspPI GGATC 2 cut(s) 42, 730
BspTI CTTAAG 1 cut(s) 286
BsrI ACTGG 2 cut(s) 271, 797
BssECI CCNNGG 4 cut(s) 7, 717, 718, 1309
BssMI GATC 5 cut(s) 34, 96, 722, 1130, 1196
BssNI GRCGYC 2 cut(s) 714, 1347
BssSI CACGAG 2 cut(s) 574, 669
Bst2BI CACGAG 2 cut(s) 574, 669
Bst2UI CCWGG 4 cut(s) 8, 719, 1311, 1362
Bst4CI ACNGT 3 cut(s) 652, 1204, 1354
BstACI GRCGYC 2 cut(s) 714, 1347
BstAFI CTTAAG 1 cut(s) 286
BstC8I GCNNGC 3 cut(s) 20, 108, 1367
BstDEI CTNAG 7 cut(s) 38, 390, 482, 1060, 1118, 1370, 1442
BstF5I GGATG 5 cut(s) 17, 338, 509, 1091, 1295
BstFNI CGCG 1 cut(s) 1419
BstKTI GATC 5 cut(s) 37, 99, 725, 1133, 1199
BstMAI GTCTC 4 cut(s) 198, 285, 669, 1432
BstMBI GATC 5 cut(s) 34, 96, 722, 1130, 1196
BstMWI GCNNNNNNNGC 2 cut(s) 542, 977
BstNI CCWGG 4 cut(s) 8, 719, 1311, 1362
BstNSI RCATGY 3 cut(s) 22, 567, 978
BstPAI GACNNNNGTC 2 cut(s) 75, 650
BstSCI CCNGG 6 cut(s) 6, 593, 717, 1104, 1309, 1360
BstUI CGCG 1 cut(s) 1419
BstV1I GCAGC 6 cut(s) 9, 353, 411, 523, 958, 1198
BstV2I GAAGAC 1 cut(s) 506
BstX2I RGATCY 3 cut(s) 34, 96, 722
BstXI CCANNNNNNTGG 1 cut(s) 1256
BstYI RGATCY 3 cut(s) 34, 96, 722
Bsu36I CCTNAGG 1 cut(s) 1118
BtsCI GGATG 5 cut(s) 17, 338, 509, 1091, 1295
BtsI GCAGTG 2 cut(s) 588, 901
BtsIMutI CAGTG 3 cut(s) 588, 901, 1068
BtuMI TCGCGA 1 cut(s) 1419
Cac8I GCNNGC 3 cut(s) 20, 108, 1367
Cfr13I GGNCC 2 cut(s) 1102, 1246
CseI GACGC 3 cut(s) 579, 722, 1355
Csp6I GTAC 2 cut(s) 4, 438
CviAII CATG 7 cut(s) 19, 31, 277, 564, 733, 854, 975
CviQI GTAC 2 cut(s) 4, 438
DdeI CTNAG 7 cut(s) 38, 390, 482, 1060, 1118, 1370, 1442
DpnI GATC 5 cut(s) 36, 98, 724, 1132, 1198
DpnII GATC 5 cut(s) 34, 96, 722, 1130, 1196
Eco32I GATATC 2 cut(s) 60, 488
Eco47I GGWCC 2 cut(s) 1102, 1246
Eco57I CTGAAG 2 cut(s) 58, 419
Eco81I CCTNAGG 1 cut(s) 1118
Eco88I CYCGRG 1 cut(s) 881
EcoRI GAATTC 1 cut(s) 1260
EcoRII CCWGG 4 cut(s) 6, 717, 1309, 1360
EcoRV GATATC 2 cut(s) 60, 488
FaeI CATG 7 cut(s) 22, 34, 280, 567, 736, 857, 978
FalI AAGNNNNNCTT 2 cut(s) 340, 372
FaqI GGGAC 2 cut(s) 568, 1011
FatI CATG 7 cut(s) 18, 30, 276, 563, 732, 853, 974
FblI GTMKAC 1 cut(s) 560
Fnu4HI GCNGC 6 cut(s) 23, 367, 425, 537, 972, 1212
FokI GGATG 5 cut(s) 24, 325, 516, 1078, 1302
Fsp4HI GCNGC 6 cut(s) 23, 367, 425, 537, 972, 1212
FspBI CTAG 6 cut(s) 219, 428, 1041, 1167, 1215, 1268
GluI GCNGC 6 cut(s) 23, 367, 425, 537, 972, 1212
GsuI CTGGAG 3 cut(s) 51, 437, 660
HapII CCGG 2 cut(s) 594, 1105
HgaI GACGC 3 cut(s) 579, 722, 1355
Hin1I GRCGYC 2 cut(s) 714, 1347
Hin1II CATG 7 cut(s) 22, 34, 280, 567, 736, 857, 978
HincII GTYRAC 2 cut(s) 264, 561
HindII GTYRAC 2 cut(s) 264, 561
HindIII AAGCTT 3 cut(s) 121, 283, 543
HinfI GANTC 9 cut(s) 27, 64, 394, 478, 635, 665, 700, 1028, 1457
HpaII CCGG 2 cut(s) 594, 1105
HphI GGTGA 2 cut(s) 128, 1216
Hpy166II GTNNAC 5 cut(s) 47, 136, 264, 561, 569
Hpy188I TCNGA 6 cut(s) 39, 354, 399, 766, 832, 942
Hpy8I GTNNAC 5 cut(s) 47, 136, 264, 561, 569
HpyAV CCTTC 3 cut(s) 469, 494, 955
HpyCH4III ACNGT 3 cut(s) 652, 1204, 1354
HpyCH4IV ACGT 2 cut(s) 1000, 1184
HpyCH4V TGCA 5 cut(s) 110, 189, 204, 297, 974
HpyF10VI GCNNNNNNNGC 2 cut(s) 542, 977
HpyF3I CTNAG 7 cut(s) 38, 390, 482, 1060, 1118, 1370, 1442
HpySE526I ACGT 2 cut(s) 1000, 1184
Hsp92I GRCGYC 2 cut(s) 714, 1347
Hsp92II CATG 7 cut(s) 22, 34, 280, 567, 736, 857, 978
Kzo9I GATC 5 cut(s) 34, 96, 722, 1130, 1196
LmnI GCTCC 2 cut(s) 421, 597
Lsp1109I GCAGC 6 cut(s) 9, 353, 411, 523, 958, 1198
LweI GCATC 1 cut(s) 1273
MaeI CTAG 6 cut(s) 219, 428, 1041, 1167, 1215, 1268
MaeII ACGT 2 cut(s) 1000, 1184
MaeIII GTNAC 5 cut(s) 643, 728, 799, 898, 1043
MalI GATC 5 cut(s) 36, 98, 724, 1132, 1198
MboI GATC 5 cut(s) 34, 96, 722, 1130, 1196
MboII GAAGA 5 cut(s) 487, 506, 785, 1121, 1457
MflI RGATCY 3 cut(s) 34, 96, 722
MlyI GAGTC 3 cut(s) 58, 709, 1466
MroXI GAANNNNTTC 1 cut(s) 164
MseI TTAA 4 cut(s) 281, 287, 359, 1485
MspCI CTTAAG 1 cut(s) 286
MspI CCGG 2 cut(s) 594, 1105
MspR9I CCNGG 6 cut(s) 8, 595, 719, 1106, 1311, 1362
Mva1269I GAATGC 2 cut(s) 204, 760
MvaI CCWGG 4 cut(s) 8, 719, 1311, 1362
MvnI CGCG 1 cut(s) 1419
MwoI GCNNNNNNNGC 2 cut(s) 542, 977
NciI CCSGG 2 cut(s) 595, 1106
NdeII GATC 5 cut(s) 34, 96, 722, 1130, 1196
NlaIII CATG 7 cut(s) 22, 34, 280, 567, 736, 857, 978
NlaIV GGNNCC 3 cut(s) 84, 599, 1089
NmuCI GTSAC 2 cut(s) 643, 728
NruI TCGCGA 1 cut(s) 1419
NspI RCATGY 3 cut(s) 22, 567, 978
PaeI GCATGC 1 cut(s) 22
PasI CCCWGGG 1 cut(s) 718
PciI ACATGT 1 cut(s) 563
PctI GAATGC 2 cut(s) 204, 760
PdmI GAANNNNTTC 1 cut(s) 164
PfeI GAWTC 6 cut(s) 27, 394, 478, 635, 665, 1028
PflMI CCANNNNNTGG 1 cut(s) 146
PkrI GCNGC 6 cut(s) 24, 368, 426, 538, 973, 1213
PleI GAGTC 3 cut(s) 58, 708, 1465
PpsI GAGTC 3 cut(s) 58, 708, 1465
PscI ACATGT 1 cut(s) 563
PshAI GACNNNNGTC 2 cut(s) 75, 650
Psp6I CCWGG 4 cut(s) 6, 717, 1309, 1360
PspGI CCWGG 4 cut(s) 6, 717, 1309, 1360
PspN4I GGNNCC 3 cut(s) 84, 599, 1089
PspPI GGNCC 2 cut(s) 1102, 1246
PsuI RGATCY 3 cut(s) 34, 96, 722
RruI TCGCGA 1 cut(s) 1419
RsaI GTAC 2 cut(s) 5, 439
RsaNI GTAC 2 cut(s) 4, 438
SalI GTCGAC 1 cut(s) 559
SaqAI TTAA 4 cut(s) 281, 287, 359, 1485
SatI GCNGC 6 cut(s) 23, 367, 425, 537, 972, 1212
Sau3AI GATC 5 cut(s) 34, 96, 722, 1130, 1196
Sau96I GGNCC 2 cut(s) 1102, 1246
SchI GAGTC 3 cut(s) 58, 709, 1466
ScrFI CCNGG 6 cut(s) 8, 595, 719, 1106, 1311, 1362
SfaNI GCATC 1 cut(s) 1273
SinI GGWCC 2 cut(s) 1102, 1246
SmlI CTYRAG 4 cut(s) 92, 197, 286, 1478
SmoI CTYRAG 4 cut(s) 92, 197, 286, 1478
SpeI ACTAGT 1 cut(s) 218
SphI GCATGC 1 cut(s) 22
SspI AATATT 1 cut(s) 532
SspMI CTAG 6 cut(s) 219, 428, 1041, 1167, 1215, 1268
StyD4I CCNGG 6 cut(s) 6, 593, 717, 1104, 1309, 1360
TaaI ACNGT 3 cut(s) 652, 1204, 1354
TaiI ACGT 2 cut(s) 1003, 1187
TaqI TCGA 4 cut(s) 62, 560, 663, 1380
TfiI GAWTC 6 cut(s) 27, 394, 478, 635, 665, 1028
Tru1I TTAA 4 cut(s) 281, 287, 359, 1485
Tru9I TTAA 4 cut(s) 281, 287, 359, 1485
TscAI CASTG 3 cut(s) 595, 901, 1068
TseFI GTSAC 2 cut(s) 643, 728
TseI GCWGC 6 cut(s) 22, 366, 424, 536, 971, 1211
Tsp45I GTSAC 2 cut(s) 643, 728
TspDTI ATGAA 4 cut(s) 19, 488, 506, 888
TspRI CASTG 3 cut(s) 595, 901, 1068
Van91I CCANNNNNTGG 1 cut(s) 146
Vha464I CTTAAG 1 cut(s) 286
VpaK11BI GGWCC 2 cut(s) 1102, 1246
XapI RAATTY 4 cut(s) 844, 876, 1260, 1402
XceI RCATGY 3 cut(s) 22, 567, 978
XmiI GTMKAC 1 cut(s) 560
XmnI GAANNNNTTC 1 cut(s) 164
XspI CTAG 6 cut(s) 219, 428, 1041, 1167, 1215, 1268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.