Rw7G041470

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000129
Physical Location & Seq
Forward (+)
64771110 .. 64787379
16270 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G041470.1

Sequence Viewer

Length: 4686 bp
ATGACTACCAGAATAGATCTTCCTTCGTCTTCTTGTCCTCCTCCTCCTCCATTCCGTAAATTGACATACGATGTGTTCCTCAGTTTCAGAGGTACAGATACCCGCACAAATTTCACAGACCATCTCTATGCTGCTCTCAAACAGAAAGGCATATACACATTTAGAGATGATGAAGAACTTAAGAGAGGGGAGACAATTGCTCCAAGTCTCTTGAAAGCAATTGAAGAGTCCAGATATGTAATTGTGGTGTTTTCTCAAAACTACGCCGATTCTGCGTGGTGTTTGGACGAGCTTGCAAAAGTCGCTGAATGCAGGAGAGCCATGGGGCAAACAGTCCTCCCTGTGTTCTATGATGTTAAGCCGTTTGAGGTACGAAGGCAAAAAGGTGATCACTTTGAGAAAGCATTTCAGAAGCATCGAGAGCGGTTTAAAGAAGAACCAGGCAAAGTAGAGAGGTGGAAAGATGCTCTTGCTGAAGTAGGCAATATCTCTGGATGGGATCTGAAAGATCGGTACGAATCCAAAGTCATTCACGAAATTGTTGAAAAGATTTTCACCGAGTTAAATCAAATATTCACAATATATGAGGATTTAGTTGGGATGGATTCTCACCTGAATGAAATGCTTTCGTACTTAGACATTGGGTGTCCTGATGTTCGTATCATAGGGATTTGTGGTATGGGTGGTATCGGTAAGACAACTATTGCACAAGTGGTTTTTGAAAGGGTACAGGCCCAGTTTGAAGGTTGCAGCTTTCTTGAGAATGTTAGAGAGGTAATTGAAAAGCAAGGTGCGGTTCATTTACTAGAGCAACTTCTTTCGAATTTGCTGGGAGGTAGTGTAAAATATAATAGGCTACATTCTAAAATGGTTCTTATCATTCTTGATGATGTGGACCAAGAAGAACAATTGGAAGCATTGTGTGATAGGGAATCGTTTGGTCCAGGAAGTAGAATCATCATAACTTGTAGAGATGAACATTTGCTCAGTGCAGTTGAAGGAGATAAAGTATATAAGGTGAATCCATTAACTGATGCTGAAGCTCTTCAGCTTTTTAGTATGAAAGCCTTCAAGAAGGACCAGCTGGTTGGAGAAAAAATTCTCAAGCTATCCAAAGAATTTTTGAAATATGCTGATGGCCTTCCACTAGCTATTAAAGTTTTGGGTTCTTCTGTTAAGGGTAGAAGAGTAGAGTTATGGTTAAGTACATTGGAAAGACTAAAAAACAATCCTAAAAAAGAAATTATCAATGTTCTAAAAGTTAGTTTTGATGGATTAGAAGAAACTGAAAAAAAAATATTTTTGGACATCGCATGTTTCTTTAAAGGATGGACCATTGCTCATGTAGCAAGGCTACTACAAGGGAGTGGCCATTGCCCGGACATTGATATAGAAGTTCTCGTAGAGAAATCTTTGGTAACTCTGTTTGGAAGAAAATTGTTGATGCATGACTTGGTACAAGAATTGGGTTGGGAAATTGTTCGTCAAGAATGCCGTGAAGAGCCTGGAAAACGTAGCAGGTTGTGGCTTCCTGATGAAATCATCCATGTACTTGGTCGGAACGATGTTGTCCACTCAATTGATAATGCCTTGTCAAATATGGACAGATTGAGATTGCTCAAGATTTATAATGTGAACTTTTCCGGAAACATCAGATATCTTTCTAATGAGTTACAGTATCTTGAATGGCATGAATGTCCTTTGGATTCTTTCCCGTCACACTTTCAACCGGATAAACTTGTTGAACTTCAAATGTGTAATAGCCGCATCAAGCAACTATGGAGGGGGAAAAAAGGATGGAGCATGCTATGGCTTATTTATCTGAGTGGTTCACAATACTTGATGTCAACTCCAGACTTCACTGAGGTTCCTGATCTTGTAGCGTTAGTGCTTCAGAATTGCACAAGCTTGGTGGAAGTTCACCCGTCTCTTGGATTTCTGAAGAAACTTCTCTATTTGGATATGACAAATTGCAAGTCTATCGAGAGCCTTCCACCTTTAACTAGCTTGGAATCCCTTGAAAAATTATGGCTTTCACTGTGTTCAGGACTGAAGAAGTTTCCAGAAATTGAAGGAAATATGAAAAGCTTGCTGGAGCTTCATTTGGATGGGACCAGCATTGAGGAATTGCCTCCATCAATTGAACGCTTGACTGGTCTAACCATGTTGGATCTGACAGATTGTAAAAACCTTTTGCATCTTCCCAATACCATTCGGTGCTTGACATCTCTGAAAAGTCTCTATCTAACAGGTTGCTCCAAAATTGATGAGATACCTGAGAATCTGAATGGTATGAAATGTCTGGAGACGCTTGCAATTGGTGGAACTTCCATAAGGGAGTTATCTTTCATTGCAGGCATGAAGAATCTACAGTATCTATCCTGCCGAGGATGTAAATGTCTAGTTTCAGAATCATGCAAAGGCCTGGCTTCGCTATCGAATTTGATACAGCTAGACTTGAGTTATTGCAATCTGATGGATGGAGCATTTCTCAATGATTTTAGCAGCCTAATCTCCTTAAAAAATTTAGATTTAAGAGGTAATTGCTTTGTGCGATTACCTGAGAGTATCTCTCAACTCTCAAAGCTCGAGACTCTGGACTTGAGTAATTGCAGACAGCTTCAATTGGTGCCCAAGAAGCTTCCGTTTAGTCTTCAAACTGTGTATGCAGCAGAATGTACTTCACTGAAGGATTACCCAAATCAAATCAAAGTATTGATGTCACGGCAGTCAGGAGTGACTATTGTCCATTCCCTCAATTCTTCAGCACTTGGAGTCCCACAAAGTTCCAGCGAGTTTTGCTTTTCAACAAATGTGGGGGAATCTAAAGGCCTGCAGGCAACTCTAGCGTTTGGAAAACTAATATATTCCCGGACAGAATTCGCTTCAGTATATCCTAGAGATGAAATTCCAGAGTGGTTCAGCACTATATCTACCAGAAATCCCATATCAATCTCGATACCTCAAAATCTGGCCGATGATAACAAGTGGCAGGGATTTGCCACATGTGTTATATTCTCAGCCAAGGGGCATACTGATATCTCCCTTATTGAACCGGATCCTGATTTTCTTAATTACTCGTATCAATTCACATTGGAAACTGATGTTGTGCGTCTGGAACCGCATATGTTCGAGGGGTATGAAGCACCCCACCAATTAAGATCAAGTTCTCATATGCTTATGATTTTCTATAAGTCTCGTTCGATAGTTCCACAGTGGGAGTTAAATCAATCAACTGTGGTGAGAGCTATATTTGAAACCACCAATCCATCCATGGTGGTCCAGAAATGTGGAATTCGTCTAGTCTATGACCAAGATGCTGAATGGTATTCCAGAATAGGCCTGTTTGATTACCCGATTTCGCTGAATGAAGTTGAGGAGGCCGCCTCTGTACGGGAGTCTACCTGGCTTAGTCAATCTTTGGAATGGGAAAAATTCATTCCACCCTTGGAAGAAGAATCGACACTTGTGCTGAGAAAGAATATTGAGTCTGTTCTTCCAAGATACCTTGAGGCACTAAACCGTTATTGTGCAACTTACAAATTTAACCTCAGAGGAAGCCCAGGGTGGTTTGAGTCGCTTACCTCTGTTAATACAAGGTTAAATGAGACCGAAGTGTCAATCAAGCTGCCTCAAAATCTACACAAGAGCAAGAAGTGGATGGGATTTGCAATATACGCATCACTTGTAGAGGAAGTGGGGCAGACAATGAAAGAGGACAACTATCGGGTACATTTTACACTAACAACTGGGGGTAAATCTCGTAAGATGATGTCCCGAGAACTTGAGCACGTCAAGCCATTGTCAGAAGAACATCATCAACTATTGGTTATTTACATACCGCAGGCACAGATTCCCGAAGCGTTGTTCACCCAGACATTAACAACTGCAATGAACTTTTACATCAAGATTAGGGATAGCTCTCATGTAAATATTCAAAGATGTGGGTTCCGTATTGTGTATCAGGAAGATATACAAGGGTTTGCTGATACAATTATCCGGTGCATGCAGAGGGAAGATTCTCTTGAATTTCATGATAAGTTGGTGGTAGAAGAATGGATACAATTGACACAATTGCAAGGTGCCCATTTAGAGAGCATGACAGAACGAGATTCCAGAACCCCACTGGAAAAATACTTTGAATTGTGCTCACGAAAATATAGAAATTGGGACTGGTCTGACCCTCGTCCTGCTTATTGTTTCTTCGAGGGAGCTGAAATTTTCAGGTCGATATGGTTCATGCCTTTCATCAACCAAGGCAACTCTGCAGAAATCCAACTGCCTCTGAATGTATTCGATGATGATAATTGGTTGGGGTTTGCCGTATGTTTTCAGTTGTCCACCGACCAATATCCAAACATTAGTGTTGGCAGTACTGCTCCTGATTCAGAGGATGTTATTATTAGTTGCCATTTGGACTCTGATGTTGCCTGGCAGCGCTTATTTGATTACCAGTTTGAATCAGGTTTAAAGATTCAAGAGCCATGGGAATGCATTGTGACTTGCTTCTGTTATATACCGCGCACTAGAGTTTGTAGTAAGGTTTGGAGGCAATGCAAATTGGCAAGGATAACTATATTGTTGTCTAGCCAACGCTTAGCAGTGTATCGTTGTGCCCTTCGTCTACTATTTAAGGAGGACATTGAATATCTTGTAGAAGCACTGACCCTCCCCGAATTACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1561

Amino Acids

178.89

Weight (kDa)

5.6

Isoelectric Point (pI)

42.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 23 - 194 1.1e-55 TIR domain
TIR_2 PF13676 25 - 126 2.4e-16 TIR domain
NB-ARC PF00931 205 - 360 3.8e-18 NB-ARC domain
WHD_ROQ1 PF23282 427 - 496 2.3e-19 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 638 - 750 6.2e-08 Leucine-rich repeat region
LRR_13 PF23286 670 - 757 4.3e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 810 - 880 6.4e-08 Leucine-rich repeat region
C-JID PF20160 966 - 1108 8.6e-10 C-JID domain
C-JID PF20160 1200 - 1325 8.6e-07 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1629
AasI GACNNNNNNGTC 1 cut(s) 3616
Acc36I ACCTGC 1 cut(s) 1509
AccB1I GGYRCC 2 cut(s) 2629, 4082
AccBSI CCGCTC 1 cut(s) 424
AccI GTMKAC 2 cut(s) 3401, 4624
AccII CGCG 1 cut(s) 4522
AccIII TCCGGA 1 cut(s) 1643
AciI CCGC 8 cut(s) 103, 424, 794, 1765, 3122, 3384, 3842, 4520
AclWI GGATC 4 cut(s) 507, 2178, 3053, 3066
AcoI YGGCCR 2 cut(s) 1369, 2973
AcuI CTGAAG 9 cut(s) 495, 1031, 1059, 1877, 1961, 2072, 2708, 2748, 2871
AfeI AGCGCT 1 cut(s) 4439
AfiI CCNNNNNNNGG 3 cut(s) 1378, 1931, 3393
AflII CTTAAG 1 cut(s) 179
AflIII ACRYGT 1 cut(s) 3005
AjiI CACGTC 1 cut(s) 3793
AjnI CCWGG 7 cut(s) 439, 943, 1504, 2424, 3404, 3562, 4430
Alw21I GWGCWC 2 cut(s) 3792, 4151
Alw26I GTCTC 8 cut(s) 185, 212, 1932, 2243, 2300, 2585, 3201, 3602
AlwI GGATC 4 cut(s) 507, 2178, 3053, 3066
Ama87I CYCGRG 2 cut(s) 2588, 3777
Aor13HI TCCGGA 1 cut(s) 1643
Aor51HI AGCGCT 1 cut(s) 4439
AoxI GGCC 8 cut(s) 732, 1138, 1369, 2422, 2830, 2973, 3340, 3381
ApeKI GCWGC 6 cut(s) 131, 750, 2505, 2669, 3628, 4435
ArsI GACNNNNNNTTYG 4 cut(s) 510, 542, 1961, 1993
Asp700I GAANNNNTTC 5 cut(s) 1044, 1067, 1098, 1479, 4292
AspLEI GCGC 2 cut(s) 4440, 4524
AspS9I GGNCC 7 cut(s) 733, 895, 941, 1078, 1332, 2112, 3280
AsuC2I CCSGG 2 cut(s) 1379, 2872
AsuHPI GGTGA 7 cut(s) 398, 547, 602, 1030, 1913, 3253, 3862
AsuII TTCGAA 1 cut(s) 821
AvaI CYCGRG 2 cut(s) 2588, 3777
AvaII GGWCC 6 cut(s) 895, 941, 1078, 1332, 2112, 3280
BaeGI GKGCMC 3 cut(s) 2634, 4087, 4618
BalI TGGCCA 1 cut(s) 1371
BamHI GGATCC 1 cut(s) 3058
BanI GGYRCC 2 cut(s) 2629, 4082
BarI GAAGNNNNNNTAC 2 cut(s) 4044, 4076
BbsI GAAGAC 2 cut(s) 21, 2645
Bbv12I GWGCWC 2 cut(s) 3792, 4151
BbvI GCAGC 6 cut(s) 118, 762, 2517, 2681, 3615, 4447
BceAI ACGGC 4 cut(s) 346, 1479, 2741, 4307
BcgI CGANNNNNNTGC 4 cut(s) 1963, 1997, 3451, 3485
BciT130I CCWGG 7 cut(s) 441, 945, 1506, 2426, 3406, 3564, 4432
BciVI GTATCC 1 cut(s) 4053
BclI TGATCA 1 cut(s) 388
BcnI CCSGG 2 cut(s) 1379, 2872
BcoDI GTCTC 8 cut(s) 185, 212, 1932, 2243, 2300, 2585, 3201, 3602
BfmI CTRYAG 3 cut(s) 2369, 2834, 4266
BfoI RGCGCY 1 cut(s) 4441
BfrI CTTAAG 1 cut(s) 179
BfuAI ACCTGC 1 cut(s) 1509
BfuI GTATCC 1 cut(s) 4053
BglII AGATCT 1 cut(s) 16
BisI GCNGC 8 cut(s) 132, 751, 1765, 2506, 2670, 3384, 3629, 4436
BlpI GCTNAGC 1 cut(s) 4597
BlsI GCNGC 8 cut(s) 133, 752, 1766, 2507, 2671, 3385, 3630, 4437
BmcAI AGTACT 1 cut(s) 4375
Bme1390I CCNGG 9 cut(s) 441, 945, 1379, 1506, 2426, 2872, 3406, 3564, 4432
Bme18I GGWCC 6 cut(s) 895, 941, 1078, 1332, 2112, 3280
BmeT110I CYCGRG 2 cut(s) 2588, 3777
BmgBI CACGTC 1 cut(s) 3793
BmgT120I GGNCC 7 cut(s) 733, 895, 941, 1078, 1332, 2112, 3280
BmiI GGNNCC 7 cut(s) 1869, 2113, 2631, 3060, 3120, 3950, 4084
BmrFI CCNGG 9 cut(s) 441, 945, 1379, 1506, 2426, 2872, 3406, 3564, 4432
BmrI ACTGGG 2 cut(s) 730, 3759
BmsI GCATC 8 cut(s) 424, 454, 1024, 1434, 1776, 2206, 3307, 3689
BmuI ACTGGG 2 cut(s) 730, 3759
BoxI GACNNNNGTC 2 cut(s) 2744, 4185
BpiI GAAGAC 2 cut(s) 21, 2645
BplI GAGNNNNNCTC 8 cut(s) 2475, 2507, 2555, 2587, 2557, 2589, 3371, 3403
BpmI CTGGAG 3 cut(s) 1836, 2114, 2324
Bpu1102I GCTNAGC 1 cut(s) 4597
Bpu14I TTCGAA 1 cut(s) 821
BpuEI CTTGAG 7 cut(s) 779, 1088, 1604, 2479, 2623, 3530, 3806
BpuMI CCSGG 2 cut(s) 1379, 2872
BsaI GGTCTC 1 cut(s) 3602
BsaJI CCNNGG 9 cut(s) 321, 2386, 3024, 3273, 3447, 3562, 3563, 4255, 4484
BsaWI WCCGGW 4 cut(s) 1643, 1729, 3055, 3999
BsaXI ACNNNNNCTCC 8 cut(s) 184, 214, 825, 855, 993, 1023, 4653, 4683
Bsc4I CCNNNNNNNGG 3 cut(s) 1378, 1931, 3393
Bse1I ACTGG 6 cut(s) 736, 2158, 3754, 4131, 4178, 4453
Bse3DI GCAATG 5 cut(s) 1335, 1372, 2349, 3897, 4559
BseAI TCCGGA 1 cut(s) 1643
BseBI CCWGG 7 cut(s) 441, 945, 1506, 2426, 3406, 3564, 4432
BseDI CCNNGG 9 cut(s) 321, 2386, 3024, 3273, 3447, 3562, 3563, 4255, 4484
BseLI CCNNNNNNNGG 3 cut(s) 1378, 1931, 3393
BseMI GCAATG 5 cut(s) 1335, 1372, 2349, 3897, 4559
BseMII CTCAG 9 cut(s) 94, 1000, 1814, 1854, 2268, 2553, 3033, 3464, 3565
BseNI ACTGG 6 cut(s) 736, 2158, 3754, 4131, 4178, 4453
BseRI GAGGAG 4 cut(s) 30, 33, 36, 3392
BseSI GKGCMC 3 cut(s) 2634, 4087, 4618
BseXI GCAGC 6 cut(s) 118, 762, 2517, 2681, 3615, 4447
BseYI CCCAGC 1 cut(s) 829
BsgI GTGCAG 1 cut(s) 1011
Bsh1236I CGCG 1 cut(s) 4522
BshFI GGCC 8 cut(s) 734, 1140, 1371, 2424, 2832, 2975, 3342, 3383
BshNI GGYRCC 2 cut(s) 2629, 4082
BsiHKAI GWGCWC 2 cut(s) 3792, 4151
BsiHKCI CYCGRG 2 cut(s) 2588, 3777
BsiSI CCGG 6 cut(s) 1379, 1644, 1730, 2872, 3056, 4000
BslFI GGGAC 4 cut(s) 2125, 2762, 3760, 4184
BslI CCNNNNNNNGG 3 cut(s) 1378, 1931, 3393
BsmAI GTCTC 8 cut(s) 185, 212, 1932, 2243, 2300, 2585, 3201, 3602
BsmBI CGTCTC 2 cut(s) 1932, 2300
BsmFI GGGAC 4 cut(s) 2125, 2762, 3760, 4184
BsmI GAATGC 3 cut(s) 314, 1496, 4496
BsnI GGCC 8 cut(s) 734, 1140, 1371, 2424, 2832, 2975, 3342, 3383
Bso31I GGTCTC 1 cut(s) 3602
BsoBI CYCGRG 2 cut(s) 2588, 3777
Bsp119I TTCGAA 1 cut(s) 821
Bsp1286I GDGCHC 5 cut(s) 2634, 3792, 4087, 4151, 4618
Bsp13I TCCGGA 1 cut(s) 1643
Bsp143I GATC 8 cut(s) 16, 388, 499, 508, 1873, 2170, 3058, 3161
Bsp1720I GCTNAGC 1 cut(s) 4597
Bsp19I CCATGG 3 cut(s) 321, 3273, 4484
BspACI CCGC 8 cut(s) 103, 424, 794, 1765, 3122, 3384, 3842, 4520
BspANI GGCC 8 cut(s) 734, 1140, 1371, 2424, 2832, 2975, 3342, 3383
BspCNI CTCAG 9 cut(s) 93, 999, 1815, 1855, 2269, 2554, 3032, 3465, 3564
BspEI TCCGGA 1 cut(s) 1643
BspFNI CGCG 1 cut(s) 4522
BspHI TCATGA 1 cut(s) 4033
BspLI GGNNCC 7 cut(s) 1869, 2113, 2631, 3060, 3120, 3950, 4084
BspMAI CTGCAG 2 cut(s) 2838, 4270
BspMI ACCTGC 1 cut(s) 1509
BspPI GGATC 4 cut(s) 507, 2178, 3053, 3066
BspQI GCTCTTC 2 cut(s) 1050, 1494
BspT104I TTCGAA 1 cut(s) 821
BspT107I GGYRCC 2 cut(s) 2629, 4082
BspTI CTTAAG 1 cut(s) 179
BspTNI GGTCTC 1 cut(s) 3602
BsrBI CCGCTC 1 cut(s) 424
BsrDI GCAATG 5 cut(s) 1335, 1372, 2349, 3897, 4559
BsrI ACTGG 6 cut(s) 736, 2158, 3754, 4131, 4178, 4453
BssECI CCNNGG 9 cut(s) 321, 2386, 3024, 3273, 3447, 3562, 3563, 4255, 4484
BssMI GATC 8 cut(s) 16, 388, 499, 508, 1873, 2170, 3058, 3161
BssT1I CCWWGG 6 cut(s) 321, 3024, 3273, 3447, 4255, 4484
Bst2UI CCWGG 7 cut(s) 441, 945, 1506, 2426, 3406, 3564, 4432
Bst4CI ACNGT 8 cut(s) 334, 1677, 2040, 2373, 2662, 3216, 3238, 3524
Bst6I CTCTTC 4 cut(s) 219, 1050, 1180, 1494
BstAFI CTTAAG 1 cut(s) 179
BstBI TTCGAA 1 cut(s) 821
BstC8I GCNNGC 9 cut(s) 294, 1805, 2090, 2313, 2356, 2834, 2838, 3846, 4007
BstDSI CCRYGG 3 cut(s) 321, 3273, 4484
BstFNI CGCG 1 cut(s) 4522
BstH2I RGCGCY 1 cut(s) 4441
BstHHI GCGC 2 cut(s) 4440, 4524
BstKTI GATC 8 cut(s) 19, 391, 502, 511, 1876, 2173, 3061, 3164
BstMAI GTCTC 8 cut(s) 185, 212, 1932, 2243, 2300, 2585, 3201, 3602
BstMBI GATC 8 cut(s) 16, 388, 499, 508, 1873, 2170, 3058, 3161
BstMWI GCNNNNNNNGC 9 cut(s) 272, 302, 421, 1346, 2638, 2799, 2846, 3677, 3796
BstNI CCWGG 7 cut(s) 441, 945, 1506, 2426, 3406, 3564, 4432
BstNSI RCATGY 4 cut(s) 1317, 1807, 3009, 4009
BstPAI GACNNNNGTC 2 cut(s) 2744, 4185
BstSCI CCNGG 9 cut(s) 439, 943, 1377, 1504, 2424, 2870, 3404, 3562, 4430
BstSFI CTRYAG 3 cut(s) 2369, 2834, 4266
BstSLI GKGCMC 3 cut(s) 2634, 4087, 4618
BstUI CGCG 1 cut(s) 4522
BstV1I GCAGC 6 cut(s) 118, 762, 2517, 2681, 3615, 4447
BstV2I GAAGAC 2 cut(s) 21, 2645
BstX2I RGATCY 4 cut(s) 16, 499, 2170, 3058
BstXI CCANNNNNNTGG 3 cut(s) 1088, 1553, 3290
BstYI RGATCY 4 cut(s) 16, 499, 2170, 3058
BsuI GTATCC 1 cut(s) 4053
BsuRI GGCC 8 cut(s) 734, 1140, 1371, 2424, 2832, 2975, 3342, 3383
BtgI CCRYGG 3 cut(s) 321, 3273, 4484
BtgZI GCGATG 1 cut(s) 1294
BtrI CACGTC 1 cut(s) 3793
BtsI GCAGTG 1 cut(s) 4608
BtsIMutI CAGTG 8 cut(s) 994, 1860, 2036, 2684, 3221, 4124, 4608, 4661
BveI ACCTGC 1 cut(s) 1509
Cac8I GCNNGC 9 cut(s) 294, 1805, 2090, 2313, 2356, 2834, 2838, 3846, 4007
CciI TCATGA 1 cut(s) 4033
CfoI GCGC 2 cut(s) 4440, 4524
Cfr13I GGNCC 7 cut(s) 733, 895, 941, 1078, 1332, 2112, 3280
CseI GACGC 2 cut(s) 2317, 3101
CspCI CAANNNNNGTGG 4 cut(s) 1893, 1928, 2796, 2831
DpnI GATC 8 cut(s) 18, 390, 501, 510, 1875, 2172, 3060, 3163
DpnII GATC 8 cut(s) 16, 388, 499, 508, 1873, 2170, 3058, 3161
DraI TTTAAA 3 cut(s) 430, 1324, 4470
DrdI GACNNNNNNGTC 1 cut(s) 3616
DseDI GACNNNNNNGTC 1 cut(s) 3616
EaeI YGGCCR 2 cut(s) 1369, 2973
Eam1104I CTCTTC 4 cut(s) 219, 1050, 1180, 1494
EarI CTCTTC 4 cut(s) 219, 1050, 1180, 1494
Eco130I CCWWGG 6 cut(s) 321, 3024, 3273, 3447, 4255, 4484
Eco147I AGGCCT 3 cut(s) 2424, 2832, 3342
Eco31I GGTCTC 1 cut(s) 3602
Eco32I GATATC 2 cut(s) 1658, 3040
Eco47I GGWCC 6 cut(s) 895, 941, 1078, 1332, 2112, 3280
Eco47III AGCGCT 1 cut(s) 4439
Eco57I CTGAAG 9 cut(s) 495, 1031, 1059, 1877, 1961, 2072, 2708, 2748, 2871
Eco88I CYCGRG 2 cut(s) 2588, 3777
EcoRI GAATTC 2 cut(s) 2879, 3294
EcoRII CCWGG 7 cut(s) 439, 943, 1504, 2424, 3404, 3562, 4430
EcoRV GATATC 2 cut(s) 1658, 3040
EcoT14I CCWWGG 6 cut(s) 321, 3024, 3273, 3447, 4255, 4484
EcoT22I ATGCAT 2 cut(s) 1449, 4496
ErhI CCWWGG 6 cut(s) 321, 3024, 3273, 3447, 4255, 4484
Esp3I CGTCTC 2 cut(s) 1932, 2300
FalI AAGNNNNNCTT 4 cut(s) 453, 485, 4008, 4040
FaqI GGGAC 4 cut(s) 2125, 2762, 3760, 4184
FauI CCCGC 1 cut(s) 110
FauNDI CATATG 2 cut(s) 3126, 3174
FbaI TGATCA 1 cut(s) 388
FblI GTMKAC 2 cut(s) 3401, 4624
Fnu4HI GCNGC 8 cut(s) 132, 751, 1765, 2506, 2670, 3384, 3629, 4436
Fsp4HI GCNGC 8 cut(s) 132, 751, 1765, 2506, 2670, 3384, 3629, 4436
GlaI GCGC 2 cut(s) 4439, 4523
GluI GCNGC 8 cut(s) 132, 751, 1765, 2506, 2670, 3384, 3629, 4436
GsaI CCCAGC 1 cut(s) 833
GsuI CTGGAG 3 cut(s) 1836, 2114, 2324
HaeII RGCGCY 1 cut(s) 4441
HaeIII GGCC 8 cut(s) 734, 1140, 1371, 2424, 2832, 2975, 3342, 3383
HapII CCGG 6 cut(s) 1379, 1644, 1730, 2872, 3056, 4000
HgaI GACGC 2 cut(s) 2317, 3101
HhaI GCGC 2 cut(s) 4440, 4524
Hin6I GCGC 2 cut(s) 4438, 4522
HinP1I GCGC 2 cut(s) 4438, 4522
HincII GTYRAC 1 cut(s) 1848
HindII GTYRAC 1 cut(s) 1848
HindIII AAGCTT 3 cut(s) 1906, 2086, 2639
HpaII CCGG 6 cut(s) 1379, 1644, 1730, 2872, 3056, 4000
HphI GGTGA 7 cut(s) 398, 547, 602, 1030, 1913, 3253, 3862
HpyCH4III ACNGT 8 cut(s) 334, 1677, 2040, 2373, 2662, 3216, 3238, 3524
HpyCH4IV ACGT 2 cut(s) 1513, 3792
HpyF10VI GCNNNNNNNGC 9 cut(s) 272, 302, 421, 1346, 2638, 2799, 2846, 3677, 3796
HpySE526I ACGT 2 cut(s) 1513, 3792
HspAI GCGC 2 cut(s) 4438, 4522
Kpn2I TCCGGA 1 cut(s) 1643
Ksp22I TGATCA 1 cut(s) 388
Kzo9I GATC 8 cut(s) 16, 388, 499, 508, 1873, 2170, 3058, 3161
LguI GCTCTTC 2 cut(s) 1050, 1494
LmnI GCTCC 7 cut(s) 205, 1800, 2095, 2261, 2483, 4211, 4384
Lsp1109I GCAGC 6 cut(s) 118, 762, 2517, 2681, 3615, 4447
LweI GCATC 8 cut(s) 424, 454, 1024, 1434, 1776, 2206, 3307, 3689
MaeII ACGT 2 cut(s) 1513, 3792
MaeIII GTNAC 6 cut(s) 1417, 1671, 1716, 2721, 2737, 4498
MalI GATC 8 cut(s) 18, 390, 501, 510, 1875, 2172, 3060, 3163
MbiI CCGCTC 1 cut(s) 424
MboI GATC 8 cut(s) 16, 388, 499, 508, 1873, 2170, 3058, 3161
MfeI CAATTG 9 cut(s) 195, 219, 908, 1578, 2139, 2316, 2624, 4064, 4073
MflI RGATCY 4 cut(s) 16, 499, 2170, 3058
MhlI GDGCHC 5 cut(s) 2634, 3792, 4087, 4151, 4618
MlsI TGGCCA 1 cut(s) 1371
MluNI TGGCCA 1 cut(s) 1371
MlyI GAGTC 7 cut(s) 236, 2587, 2784, 3407, 3497, 3584, 4412
MmeI TCCRAC 4 cut(s) 1069, 1538, 2148, 4300
Mox20I TGGCCA 1 cut(s) 1371
Mph1103I ATGCAT 2 cut(s) 1449, 4496
MroI TCCGGA 1 cut(s) 1643
MroXI GAANNNNTTC 5 cut(s) 1044, 1067, 1098, 1479, 4292
MscI TGGCCA 1 cut(s) 1371
MslI CAYNNNNRTG 4 cut(s) 126, 615, 2106, 4489
Msp20I TGGCCA 1 cut(s) 1371
MspA1I CMGCKG 1 cut(s) 1084
MspCI CTTAAG 1 cut(s) 179
MspI CCGG 6 cut(s) 1379, 1644, 1730, 2872, 3056, 4000
MspR9I CCNGG 9 cut(s) 441, 945, 1379, 1506, 2426, 2872, 3406, 3564, 4432
MunI CAATTG 9 cut(s) 195, 219, 908, 1578, 2139, 2316, 2624, 4064, 4073
Mva1269I GAATGC 3 cut(s) 314, 1496, 4496
MvaI CCWGG 7 cut(s) 441, 945, 1506, 2426, 3406, 3564, 4432
MvnI CGCG 1 cut(s) 4522
MwoI GCNNNNNNNGC 9 cut(s) 272, 302, 421, 1346, 2638, 2799, 2846, 3677, 3796
NciI CCSGG 2 cut(s) 1379, 2872
NcoI CCATGG 3 cut(s) 321, 3273, 4484
NdeI CATATG 2 cut(s) 3126, 3174
NdeII GATC 8 cut(s) 16, 388, 499, 508, 1873, 2170, 3058, 3161
NlaIV GGNNCC 7 cut(s) 1869, 2113, 2631, 3060, 3120, 3950, 4084
NmeAIII GCCGAG 1 cut(s) 2411
NmuCI GTSAC 4 cut(s) 1716, 2721, 2737, 4498
NsiI ATGCAT 2 cut(s) 1449, 4496
NspI RCATGY 4 cut(s) 1317, 1807, 3009, 4009
NspV TTCGAA 1 cut(s) 821
PaeI GCATGC 2 cut(s) 1807, 4009
PaeR7I CTCGAG 1 cut(s) 2588
PagI TCATGA 1 cut(s) 4033
PasI CCCWGGG 1 cut(s) 3563
PceI AGGCCT 3 cut(s) 2424, 2832, 3342
PciI ACATGT 1 cut(s) 3005
PciSI GCTCTTC 2 cut(s) 1050, 1494
PctI GAATGC 3 cut(s) 314, 1496, 4496
PdmI GAANNNNTTC 5 cut(s) 1044, 1067, 1098, 1479, 4292
PfoI TCCNGGA 2 cut(s) 943, 2870
PkrI GCNGC 8 cut(s) 133, 752, 1766, 2507, 2671, 3385, 3630, 4437
PleI GAGTC 7 cut(s) 235, 2587, 2783, 3406, 3496, 3583, 4412
PpsI GAGTC 7 cut(s) 235, 2587, 2783, 3406, 3496, 3583, 4412
PscI ACATGT 1 cut(s) 3005
PshAI GACNNNNGTC 2 cut(s) 2744, 4185
PsiI TTATAA 1 cut(s) 1629
Psp6I CCWGG 7 cut(s) 439, 943, 1504, 2424, 3404, 3562, 4430
PspFI CCCAGC 1 cut(s) 829
PspGI CCWGG 7 cut(s) 439, 943, 1504, 2424, 3404, 3562, 4430
PspN4I GGNNCC 7 cut(s) 1869, 2113, 2631, 3060, 3120, 3950, 4084
PspPI GGNCC 7 cut(s) 733, 895, 941, 1078, 1332, 2112, 3280
PstI CTGCAG 2 cut(s) 2838, 4270
PsuI RGATCY 4 cut(s) 16, 499, 2170, 3058
PvuII CAGCTG 1 cut(s) 1084
RseI CAYNNNNRTG 4 cut(s) 126, 615, 2106, 4489
SapI GCTCTTC 2 cut(s) 1050, 1494
SatI GCNGC 8 cut(s) 132, 751, 1765, 2506, 2670, 3384, 3629, 4436
Sau3AI GATC 8 cut(s) 16, 388, 499, 508, 1873, 2170, 3058, 3161
Sau96I GGNCC 7 cut(s) 733, 895, 941, 1078, 1332, 2112, 3280
SbfI CCTGCAGG 1 cut(s) 2838
ScaI AGTACT 1 cut(s) 4375
SchI GAGTC 7 cut(s) 236, 2587, 2784, 3407, 3497, 3584, 4412
ScrFI CCNGG 9 cut(s) 441, 945, 1379, 1506, 2426, 2872, 3406, 3564, 4432
SdaI CCTGCAGG 1 cut(s) 2838
SduI GDGCHC 5 cut(s) 2634, 3792, 4087, 4151, 4618
SfaNI GCATC 8 cut(s) 424, 454, 1024, 1434, 1776, 2206, 3307, 3689
SfcI CTRYAG 3 cut(s) 2369, 2834, 4266
Sfr274I CTCGAG 1 cut(s) 2588
SfuI TTCGAA 1 cut(s) 821
SinI GGWCC 6 cut(s) 895, 941, 1078, 1332, 2112, 3280
SlaI CTCGAG 1 cut(s) 2588
SmiMI CAYNNNNRTG 4 cut(s) 126, 615, 2106, 4489
SmlI CTYRAG 9 cut(s) 179, 758, 1103, 1619, 2458, 2588, 2602, 3509, 3785
SmoI CTYRAG 9 cut(s) 179, 758, 1103, 1619, 2458, 2588, 2602, 3509, 3785
SphI GCATGC 2 cut(s) 1807, 4009
Sse8387I CCTGCAGG 1 cut(s) 2838
SseBI AGGCCT 3 cut(s) 2424, 2832, 3342
SsiI CCGC 8 cut(s) 103, 424, 794, 1765, 3122, 3384, 3842, 4520
SspI AATATT 4 cut(s) 573, 1299, 3484, 3934
StuI AGGCCT 3 cut(s) 2424, 2832, 3342
StyD4I CCNGG 9 cut(s) 439, 943, 1377, 1504, 2424, 2870, 3404, 3562, 4430
StyI CCWWGG 6 cut(s) 321, 3024, 3273, 3447, 4255, 4484
TaaI ACNGT 8 cut(s) 334, 1677, 2040, 2373, 2662, 3216, 3238, 3524
TaiI ACGT 2 cut(s) 1516, 3795
TaqII GACCGA 1 cut(s) 3626
TatI WGTACW 4 cut(s) 1205, 1549, 2678, 4373
TauI GCSGC 2 cut(s) 1767, 3386
TscAI CASTG 8 cut(s) 994, 1867, 2043, 2691, 3221, 4131, 4608, 4668
TseFI GTSAC 4 cut(s) 1716, 2721, 2737, 4498
TseI GCWGC 6 cut(s) 131, 750, 2505, 2669, 3628, 4435
Tsp45I GTSAC 4 cut(s) 1716, 2721, 2737, 4498
TspGWI ACGGA 3 cut(s) 44, 2634, 3941
TspRI CASTG 8 cut(s) 994, 1867, 2043, 2691, 3221, 4131, 4608, 4668
Vha464I CTTAAG 1 cut(s) 179
VpaK11BI GGWCC 6 cut(s) 895, 941, 1078, 1332, 2112, 3280
XceI RCATGY 4 cut(s) 1317, 1807, 3009, 4009
XcmI CCANNNNNNNNNTGG 2 cut(s) 3271, 4123
XhoI CTCGAG 1 cut(s) 2588
XmiI GTMKAC 2 cut(s) 3401, 4624
XmnI GAANNNNTTC 5 cut(s) 1044, 1067, 1098, 1479, 4292
ZrmI AGTACT 1 cut(s) 4375
Zsp2I ATGCAT 2 cut(s) 1449, 4496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.