Rh7AG361400

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0000129
Physical Location & Seq
Forward (+)
46489839 .. 46497710
7872 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG361400.1

Sequence Viewer

Length: 5007 bp
ATGATCACCCACACAGATATTCCTTCTTCTTCTTCCTCTCCTTCTCCGATCCCTCGATGGACGTACGACGTGTTCCTCAGTTTCAGGGGCGAAGATACCCGAAAAACTTTTACAGACCATCTCTACACCAGTCTTAAACAGAAAGGCATAATCACGTTCAGAGACGATGAGGGACTTGAGAGAGGAAAAGCCATTTCCCCAAATCTGTTGAAAGCCATTGAAGAATCAAGATTCGTCATTGCCATCTTTTCAAGTAAGTACGCTGATTCAAGATGGTGCTTGGATGAGCTCGCAAAGGCTGTTGATTGTATGAACATGTTGGGACAAACGATACTCCCAGTCTTTTACCATGTGGATCCATCTGAGGTGAGAAAACAAACAGGAAAATTCGGCCAAGACTTGTGCATGCATCAACAACGCTTTAAAGACAAAATAGAGCAGGTGAATAGGTGGAGAGCTGCTCTTACTCAAGTCGCCAATCTCTCTGGATGGCATTTGCAGGATGGATCATATGAATCAAAAGTAATTCAAGCGATTGTAGGGCATATTTTCAACAAGTTGAACGAAACAATCTCAAGTGGATCCATGGATTTAGTTGGTATCGATTCTCGAATAAAACAAATCCTTTCATACTTAGACTTGGAGTTGAATGAAGTATGTATGATAGGTATTTCGGGAATGGGGGGAATCGGTAAGACAACTACTGCACAGGTAGTTTATGAAAGGATACGTGCTCAGTTTGAGGCTTACAGCTTTCTTGCAAATGTTAGAGAGACAACTGAGAAACAGGGCCTAGTTCACTTACAAAAACAACTTCTTTCAGACATTTTGCTGGAACGTAATGTAAATATACAGAATATTGATATGGGAGTTAATATAATAAGCCAGAGACTACGAACTAAAATGGTTCTTATCATTCTTGATGATGTGAATGACTTGCAACAACTGAAAGCATTGTGTGGTCATAGTTGGTTTGGTTCTGGGAGCAGGATCATAATAACATCAAGGGATGAACAATTGCTTCGCACATATGGAGTGGAAAGCATATACAAGGTCAAGGCATTAAACGACGCGGAAGCTCTTCAGCTCTTTAGTTGGAAAGCATTCAGGAAAGATAAGGTTGGAGAAGATTTTCTCGAGCTATCAAAGAGTGTTTTAGAATATGCTAATGGGCTTCCATTAGCTATTGAAGTTTTGGGTTCCTTACTCTTTGGTAAAAGTGTAAAAGTATGGACAAGTGCATTGGATAGACTTAAAGTTAATCCTGACAAAAGAATAATTGATGTTCTTAAAGTAACTTTTGATGCATTAGAAGACACAGAAAAGGAAGTCTTTCTGGACATTGCATGTTTCTTTAAAGGGGAGGACAGAGATCGTGTAACAAGAATACTAGAAAGTAGTTGTGGCTACTCTCCAGACATTGATATAGAAGTTCTCATTGATAAGTCGATGGTAACTCTATTTGGAAGACAATTGTGGATGCATGACTTGATACAAGAATTGGGTTGGGAAATTGTTCGTCGGGAATGTCGTGATGAGCCGTGCAAACGTAGCAGGTTGTGGCTTCCAAAAGATATCATTCCTTTACTTGAACATAATCTGGGAACGAGTGCAGTGGAATCTATTTTGCTGAATTTGCCAAAACAAAAGGAGGTTCACTTGAATGTTGATCCCTTCTCAAAGATGTGCAAGTTAAGATTGCTCAAAATTAATAATGCACGCTTTTCTGGAGACATCAAATGGCTTTCTAATGAGTTACAAGTTCTTGAATGGCATGTTTGTCCTTTATATTCTTTGCCATCAAACTTTCAATCAGATAAGCTTGTTGAACTTAGCATGCATTCAAGCTGTATCAAACAGCTATGGAAGGGAAATAAGCGGTTGAGCATGCTAAAGTTCATCGATATGAGTGATTCTCAATACTTGATCAGGACCCCAAGCTTTGTTGAGGTCCCAAACATTGAGACTTTGGTGCTTGAAGGCTGTACAAGATTGGTTGAGGTTGACCCATCCATTGGAGTTCTCAAAAGACTTAATTTGTTGAATATGAGAAATTGCAAATCCCTTAAGAGCCTTCCACCTAACATTAGTTTGGAATCCCTTACAATCTTTAATCTCTCTGGCTGCTCAAGCTTGAAGAAGTTTCCAGAAATTGAAGGAAACATGCAGAGCTTGTTGGAGCTTCATCTAGATGGGACTGCCATAGAGGAATTACCTGCATCAATTGAAAGTTTAACTGGTCTCACTTTGTTGAATTTAGGAGACTGCACAAATCTGTTCCGGCTTCCAAGTACCATTCAATTCTTGACATCTCTGAAAAGTCTCATTCTTACAGGTTGCTCAGAACTTGGAGACATACCAGAGAATCTGAATTGTGTGGAATGTTTGGAGGATCTTGATATAAGTGGAACTGCTATAAGAGAGTTATCGTGCATTGCAGATATGAAAAATCTTAAGTCTCTATGTTTCAGAGGAGGTAAAAATCTACCTCCGAAATCCTGGCATTCACTGTTCAATTGTTGGGGGATGTTTGGAAGGAAAGATGTTCCTGTGGGCTTGTTGCTGCCCGCTTCCTTATCAGGTTTGAGTTCTTTGACATATCTAAACTTCAGTGAATGCAATCTGATGGATGGAGATATTCCAAATGATCTTGGGAGTTTAGTCTCTCTAAGAAAATTGATTTTAAGTGGTAATAAGTTTTCTTGCTTACCGGAAAGCATCTCTCAGCTCTCTAAGCTTCAAACTCTCGACTTGAACAATTGTAGCCGGCTTCAATCGCTGCCTAAGAAACTCCCATTAAGTGTTCGCGCTGTGAATGCAGATGACTGTACATCACTGATGGATTATCCAGATCAATTCTTAGTATGGACTTCATCTGTGTCAGGAATGACAACTATGAGTTCATTGATTTCATCCAAGCATAAAAAATATTCCACTTCCTCACCAAGACAAATCTCATATCATTCCAGAACATGGATTCAGTCAACCGTCCTTGGACGTGGTTTCTTTAGTTTTAGCCATCAACAAGAATGTAAACCAGTTGATGATTTATCAATGCCAAATCAAGTGCTTCAGAAAGACCTTGAAGCATTGGATTACAAGTCTTGTTCCCTGATTTCCGGCTGTGAAGAAAATGAAATTCCAGAGTGGTTTAGCAATATAGATACCGGCAGCTCCGTAGGAATCTCGATACCTGCAGGACTCAAAGATGATGAGCAATGGATGGGGGTTTCTGCTGTTTTCTTAATCAAGGGACATCCTCATTTCTCCGACATTGAATCAGAATCAGAAACACCTACTTACTTCTATAAATGCACAGTAGTAACTCAAGAATGTCAAGTGGAACCTGTTCTCCTTGAATGGAACCATCTCAACTGCATTCAATATAGTTCTCAGTTTCTTTGCTTTTTCTATGTACCTTGCCAATTCTTTCCCAAAAGGTTAAATGAATCAAGTGTAATGTGGGCTGTCTTTGAGACCAATAACCCATGCATGGAGGTCCAGAAATGTGGGATTCGTCTACTGTTCAAGCAAGGTGTTGCAGGGTTTATCCAGACATTCATGCCTCCCACTCTTGGTATTGGAGGTCGTACGCATGATCTTGACCCTCTGCTTGCAATAAATGACAGCCGAATCGTCCCCCCTGACGACTGCATCAATCTGATTGAAGTTGACAAAGCTACAATTGATTCAGGATGGTTTAATCTAGTAGTGGATCGCACTTTGAGATGGGAGAAATTACTTCCAACTTATGAAGAAGGCTCCAAACTGCCTAGAATTAGGAACATGGAGTCATTTCTATCAAGATTCCTTGAGGGACTAAACCATAAGGCTCAACCCTATGATTTTCTTGTAAGTGGAAGCCCAGCGTGGTTCAAGACTGAAGTTGGGTCCACGGTGTCAATCCAGTTGCCCCCTCATGAGAATCTACACAAGAGTAAAAAGTGGATGGGATTTGCTCTATGCACATCACTCGTTGTGGACAAGCATACAGCAAAGGAGGATTATGTTGGTTCTTGCCGATTCCAAAGGGGCAGATCTCATACCATTGGATTTAATCTTACCCTGGACTTTAGACCCTTCATGATAGCTGATGCACATCAACTTTTGGTTTTTTATTTACCGCGGGCAGTGATTCCCCAACTGTTCATTGAAGCATCATCAAGAGAAATATTCACTTCCTATGAAACCGGTAGCCCTGGTGTAAAGGTTCAAATTTGTGGACTTCGTATTTTGTACAAGCAAGATTTACAAGGGTTTGTCCAAACAATCACCCACTGCATATTACGCAGCTCAGGTGCCCCCTTTGAAGTTTACAATAAACTGGTGGTAGAAGATTGGTTAAGTTTGGTAAGGTTGCCGAGTCATAAATTCAATGCTTCCCGAGATTGTGATTCCAGTACCCGCCATAAAAGACAGTTTGAGTTGATCTCTCAAAGCTATAGAAATATGGACTGGTCTGTGGAAAACCTGTCATGCACGTTCCCTGGAATTGAAATTCCTGAGTGGTTCATAAATGTGGAAAAAAGCGACTCTGCTAAAATGCAACTAAATGGTAATTGGATGGGAATTGCAGTGTGTGCTTCTCTATCGATCCACGAGCATCCGAATATCATTTTTGACAATCCGTATGCAGAGTCTACTCGTCACGACATTTTTTGTTATCTAAAGGGCAATATTGCTTCTGCTGGTTCTCTCCGTTATAAGTTTGATACAGACAAAGAAATGTGGATGCATCCATGCAATTTCATTTGCTTTGTCTATATGCCACGGGCTTATTTTCCTGATTCATGGAATCAATGTGATACAATCGAGGGAAGTATTGGGTCTGACAGCTCAGGCTTTGGAATTCACAAGTGTGCCCTTGGTCTAGTATTTGAGGAGGATGTGGAGGATCTTGTACGAACGTTGACTTACTGCCACTTATCAGCGCACTGTCATCAGTGTGAAGAAGCACAATATCTTAGTCAAAAGAGGTACCAGAAACCATCGAAAATTAGTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

1668

Amino Acids

189.02

Weight (kDa)

5.99

Isoelectric Point (pI)

51.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 22 - 195 9.5e-59 TIR domain
TIR_2 PF13676 24 - 121 7.8e-16 TIR domain
NB-ARC PF00931 205 - 372 1.9e-19 NB-ARC domain
NACHT PF05729 224 - 364 4.6e-06 NACHT domain
WHD_ROQ1 PF23282 439 - 508 1.9e-17 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 690 - 823 6.6e-11 Leucine-rich repeat region
LRR_13 PF23286 698 - 784 2.4e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 857 - 930 1.3e-08 Leucine-rich repeat region
LRR_13 PF23286 864 - 948 3.4e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4704
AarI CACCTGC 1 cut(s) 430
Acc36I ACCTGC 4 cut(s) 430, 1545, 2224, 3202
Acc65I GGTACC 1 cut(s) 4977
AccB1I GGYRCC 2 cut(s) 4298, 4977
AccB7I CCANNNNNTGG 2 cut(s) 2015, 2973
AccI GTMKAC 2 cut(s) 3520, 4640
AccII CGCG 3 cut(s) 1073, 2808, 4126
AciI CCGC 6 cut(s) 1073, 1879, 2568, 4124, 4126, 4405
AclI AACGTT 1 cut(s) 4907
AcoI YGGCCR 1 cut(s) 391
AcsI RAATTY 8 cut(s) 386, 1633, 2254, 3138, 4215, 4370, 4497, 4848
AcuI CTGAAG 4 cut(s) 1067, 2593, 3056, 3903
AfiI CCNNNNNNNGG 4 cut(s) 2015, 2973, 3493, 3575
AflII CTTAAG 2 cut(s) 2066, 2453
AflIII ACRYGT 2 cut(s) 69, 315
AgeI ACCGGT 1 cut(s) 4190
AjiI CACGTC 2 cut(s) 70, 2999
AjnI CCWGG 4 cut(s) 2498, 4065, 4198, 4486
AleI CACNNNNGTG 1 cut(s) 4857
AloI GAACNNNNNNTCC 2 cut(s) 3336, 3368
Alw21I GWGCWC 2 cut(s) 291, 736
Ama87I CYCGRG 2 cut(s) 1136, 4383
AoxI GGCC 2 cut(s) 391, 790
ApeKI GCWGC 6 cut(s) 458, 2124, 2563, 2779, 3171, 4290
ApoI RAATTY 8 cut(s) 386, 1633, 2254, 3138, 4215, 4370, 4497, 4848
AseI ATTAAT 1 cut(s) 1710
AsiGI ACCGGT 1 cut(s) 4190
Asp700I GAANNNNTTC 6 cut(s) 1080, 1103, 1131, 1332, 1515, 3348
Asp718I GGTACC 1 cut(s) 4977
AspLEI GCGC 2 cut(s) 2810, 4933
AspS9I GGNCC 5 cut(s) 790, 1932, 1951, 3499, 3891
AsuHPI GGTGA 4 cut(s) 379, 454, 2934, 4264
AvaI CYCGRG 2 cut(s) 1136, 4383
AvaII GGWCC 4 cut(s) 1932, 1951, 3499, 3891
BaeGI GKGCMC 2 cut(s) 4303, 4864
BamHI GGATCC 2 cut(s) 355, 581
BanI GGYRCC 2 cut(s) 4298, 4977
BanII GRGCYC 1 cut(s) 291
BauI CACGAG 1 cut(s) 4598
BbsI GAAGAC 2 cut(s) 1320, 1474
Bbv12I GWGCWC 2 cut(s) 291, 736
BbvI GCAGC 6 cut(s) 445, 2111, 2550, 2766, 3183, 4302
BceAI ACGGC 1 cut(s) 1525
BcgI CGANNNNNNTGC 2 cut(s) 3955, 3989
BciT130I CCWGG 4 cut(s) 2500, 4067, 4200, 4488
BciVI GTATCC 1 cut(s) 720
BclI TGATCA 2 cut(s) 3, 1926
BfaI CTAG 6 cut(s) 794, 1391, 2189, 3707, 3774, 4871
BfmI CTRYAG 2 cut(s) 3195, 4441
BfrI CTTAAG 2 cut(s) 2066, 2453
BfuAI ACCTGC 4 cut(s) 430, 1545, 2224, 3202
BfuI GTATCC 1 cut(s) 720
BglII AGATCT 1 cut(s) 4037
BisI GCNGC 6 cut(s) 459, 2125, 2564, 2780, 3172, 4291
BlsI GCNGC 6 cut(s) 460, 2126, 2565, 2781, 3173, 4292
Bme1390I CCNGG 4 cut(s) 2500, 4067, 4200, 4488
Bme18I GGWCC 4 cut(s) 1932, 1951, 3499, 3891
BmeT110I CYCGRG 2 cut(s) 1136, 4383
BmgBI CACGTC 2 cut(s) 70, 2999
BmgT120I GGNCC 5 cut(s) 790, 1932, 1951, 3499, 3891
BmrFI CCNGG 4 cut(s) 2500, 4067, 4200, 4488
BmrI ACTGGG 1 cut(s) 332
BmuI ACTGGG 1 cut(s) 332
BpiI GAAGAC 2 cut(s) 1320, 1474
BplI GAGNNNNNCTC 6 cut(s) 445, 477, 1900, 1932, 4415, 4447
BpmI CTGGAG 2 cut(s) 1398, 1749
Bpu10I CCTNAGC 2 cut(s) 4294, 4837
BpuEI CTTGAG 6 cut(s) 197, 453, 559, 2113, 3312, 3833
Bsa29I ATCGAT 3 cut(s) 603, 1902, 4592
BsaAI YACGTR 1 cut(s) 731
BsaBI GATNNNNATC 1 cut(s) 4098
BsaI GGTCTC 2 cut(s) 2246, 3470
BsaJI CCNNGG 9 cut(s) 585, 2992, 3894, 4065, 4124, 4198, 4486, 4769, 4864
BsaWI WCCGGW 2 cut(s) 2710, 4190
BsaXI ACNNNNNCTCC 2 cut(s) 3336, 3366
Bsc4I CCNNNNNNNGG 4 cut(s) 2015, 2973, 3493, 3575
Bse118I RCCGGY 3 cut(s) 2766, 3167, 4190
Bse1I ACTGG 8 cut(s) 129, 338, 2242, 3038, 3907, 4329, 4398, 4460
Bse3DI GCAATG 4 cut(s) 237, 1341, 2433, 3224
Bse8I GATNNNNATC 1 cut(s) 4098
BseBI CCWGG 4 cut(s) 2500, 4067, 4200, 4488
BseCI ATCGAT 3 cut(s) 603, 1902, 4592
BseDI CCNNGG 9 cut(s) 585, 2992, 3894, 4065, 4124, 4198, 4486, 4769, 4864
BseJI GATNNNNATC 1 cut(s) 4098
BseLI CCNNNNNNNGG 4 cut(s) 2015, 2973, 3493, 3575
BseMI GCAATG 4 cut(s) 237, 1341, 2433, 3224
BseNI ACTGG 8 cut(s) 129, 338, 2242, 3038, 3907, 4329, 4398, 4460
BseRI GAGGAG 2 cut(s) 2487, 4895
BseSI GKGCMC 2 cut(s) 4303, 4864
BseXI GCAGC 6 cut(s) 445, 2111, 2550, 2766, 3183, 4302
BseYI CCCAGC 1 cut(s) 3865
BsgI GTGCAG 3 cut(s) 690, 1632, 2251
Bsh1236I CGCG 3 cut(s) 1073, 2808, 4126
BshFI GGCC 2 cut(s) 393, 792
BshNI GGYRCC 2 cut(s) 4298, 4977
BshTI ACCGGT 1 cut(s) 4190
BshVI ATCGAT 3 cut(s) 603, 1902, 4592
BsiHKAI GWGCWC 2 cut(s) 291, 736
BsiHKCI CYCGRG 2 cut(s) 1136, 4383
BsiSI CCGG 6 cut(s) 2281, 2711, 2767, 3120, 3168, 4191
BsiWI CGTACG 2 cut(s) 63, 3590
BslFI GGGAC 7 cut(s) 186, 336, 1937, 2209, 3267, 3623, 3831
BslI CCNNNNNNNGG 4 cut(s) 2015, 2973, 3493, 3575
BsmBI CGTCTC 1 cut(s) 156
BsmFI GGGAC 7 cut(s) 186, 336, 1937, 2209, 3267, 3623, 3831
BsmI GAATGC 6 cut(s) 1103, 1840, 2503, 2621, 2821, 3378
BsnI GGCC 2 cut(s) 393, 792
Bso31I GGTCTC 2 cut(s) 2246, 3470
BsoBI CYCGRG 2 cut(s) 1136, 4383
Bsp1286I GDGCHC 4 cut(s) 291, 736, 4303, 4864
Bsp1407I TGTACA 3 cut(s) 1985, 2828, 4236
Bsp19I CCATGG 1 cut(s) 585
BspACI CCGC 6 cut(s) 1073, 1879, 2568, 4124, 4126, 4405
BspANI GGCC 2 cut(s) 393, 792
BspDI ATCGAT 3 cut(s) 603, 1902, 4592
BspFNI CGCG 3 cut(s) 1073, 2808, 4126
BspHI TCATGA 2 cut(s) 3919, 4083
BspMAI CTGCAG 1 cut(s) 3199
BspMI ACCTGC 4 cut(s) 430, 1545, 2224, 3202
BspQI GCTCTTC 1 cut(s) 1086
BspT107I GGYRCC 2 cut(s) 4298, 4977
BspTI CTTAAG 2 cut(s) 2066, 2453
BspTNI GGTCTC 2 cut(s) 2246, 3470
BsrDI GCAATG 4 cut(s) 237, 1341, 2433, 3224
BsrFI RCCGGY 3 cut(s) 2766, 3167, 4190
BsrGI TGTACA 3 cut(s) 1985, 2828, 4236
BsrI ACTGG 8 cut(s) 129, 338, 2242, 3038, 3907, 4329, 4398, 4460
BssAI RCCGGY 3 cut(s) 2766, 3167, 4190
BssECI CCNNGG 9 cut(s) 585, 2992, 3894, 4065, 4124, 4198, 4486, 4769, 4864
BssSI CACGAG 1 cut(s) 4598
BssT1I CCWWGG 3 cut(s) 585, 2992, 4864
Bst2BI CACGAG 1 cut(s) 4598
Bst2UI CCWGG 4 cut(s) 2500, 4067, 4200, 4488
Bst4CI ACNGT 9 cut(s) 2511, 2828, 2989, 3319, 3525, 3898, 4146, 4419, 4937
Bst6I CTCTTC 1 cut(s) 1086
BstAFI CTTAAG 2 cut(s) 2066, 2453
BstAPI GCANNNNNTGC 1 cut(s) 4580
BstAUI TGTACA 3 cut(s) 1985, 2828, 4236
BstBAI YACGTR 1 cut(s) 731
BstC8I GCNNGC 9 cut(s) 291, 407, 1720, 1838, 1889, 2568, 2768, 3615, 4128
BstDSI CCRYGG 4 cut(s) 585, 3894, 4124, 4769
BstFNI CGCG 3 cut(s) 1073, 2808, 4126
BstHHI GCGC 2 cut(s) 2810, 4933
BstMWI GCNNNNNNNGC 8 cut(s) 1551, 1636, 2130, 2734, 2776, 2816, 4287, 4580
BstNI CCWGG 4 cut(s) 2500, 4067, 4200, 4488
BstNSI RCATGY 7 cut(s) 319, 409, 1350, 1778, 1840, 1891, 2167
BstSCI CCNGG 4 cut(s) 2498, 4065, 4198, 4486
BstSFI CTRYAG 2 cut(s) 3195, 4441
BstSLI GKGCMC 2 cut(s) 4303, 4864
BstUI CGCG 3 cut(s) 1073, 2808, 4126
BstV1I GCAGC 6 cut(s) 445, 2111, 2550, 2766, 3183, 4302
BstV2I GAAGAC 2 cut(s) 1320, 1474
BstX2I RGATCY 5 cut(s) 355, 581, 2392, 4037, 4894
BstXI CCANNNNNNTGG 1 cut(s) 3509
BstYI RGATCY 5 cut(s) 355, 581, 2392, 4037, 4894
Bsu15I ATCGAT 3 cut(s) 603, 1902, 4592
BsuI GTATCC 1 cut(s) 720
BsuRI GGCC 2 cut(s) 393, 792
BsuTUI ATCGAT 3 cut(s) 603, 1902, 4592
BtgI CCRYGG 4 cut(s) 585, 3894, 4124, 4769
BtrI CACGTC 2 cut(s) 70, 2999
BtsI GCAGTG 4 cut(s) 1620, 4137, 4276, 4581
BtsIMutI CAGTG 9 cut(s) 1620, 2507, 2617, 2834, 4137, 4276, 4581, 4933, 4949
BveI ACCTGC 4 cut(s) 430, 1545, 2224, 3202
Cac8I GCNNGC 9 cut(s) 291, 407, 1720, 1838, 1889, 2568, 2768, 3615, 4128
CciI TCATGA 2 cut(s) 3919, 4083
CfoI GCGC 2 cut(s) 2810, 4933
Cfr10I RCCGGY 3 cut(s) 2766, 3167, 4190
Cfr13I GGNCC 5 cut(s) 790, 1932, 1951, 3499, 3891
Cfr42I CCGCGG 1 cut(s) 4127
ClaI ATCGAT 3 cut(s) 603, 1902, 4592
CseI GACGC 1 cut(s) 1079
CspAI ACCGGT 1 cut(s) 4190
DraI TTTAAA 2 cut(s) 424, 1357
EaeI YGGCCR 1 cut(s) 391
Eam1104I CTCTTC 1 cut(s) 1086
EarI CTCTTC 1 cut(s) 1086
Ecl136II GAGCTC 1 cut(s) 289
Eco130I CCWWGG 3 cut(s) 585, 2992, 4864
Eco24I GRGCYC 1 cut(s) 291
Eco31I GGTCTC 2 cut(s) 2246, 3470
Eco32I GATATC 1 cut(s) 1576
Eco47I GGWCC 4 cut(s) 1932, 1951, 3499, 3891
Eco53kI GAGCTC 1 cut(s) 289
Eco57I CTGAAG 4 cut(s) 1067, 2593, 3056, 3903
Eco88I CYCGRG 2 cut(s) 1136, 4383
EcoICRI GAGCTC 1 cut(s) 289
EcoO109I RGGNCCY 3 cut(s) 790, 1932, 1951
EcoRI GAATTC 1 cut(s) 4848
EcoRII CCWGG 4 cut(s) 2498, 4065, 4198, 4486
EcoRV GATATC 1 cut(s) 1576
EcoT14I CCWWGG 3 cut(s) 585, 2992, 4864
EcoT22I ATGCAT 6 cut(s) 411, 1309, 1485, 1842, 3494, 4737
EcoT38I GRGCYC 1 cut(s) 291
ErhI CCWWGG 3 cut(s) 585, 2992, 4864
Esp3I CGTCTC 1 cut(s) 156
FalI AAGNNNNNCTT 2 cut(s) 1316, 1348
FaqI GGGAC 7 cut(s) 186, 336, 1937, 2209, 3267, 3623, 3831
FauI CCCGC 3 cut(s) 2575, 4119, 4412
FauNDI CATATG 2 cut(s) 511, 1030
FbaI TGATCA 2 cut(s) 3, 1926
FblI GTMKAC 2 cut(s) 3520, 4640
Fnu4HI GCNGC 6 cut(s) 459, 2125, 2564, 2780, 3172, 4291
FriOI GRGCYC 1 cut(s) 291
Fsp4HI GCNGC 6 cut(s) 459, 2125, 2564, 2780, 3172, 4291
FspBI CTAG 6 cut(s) 794, 1391, 2189, 3707, 3774, 4871
GlaI GCGC 2 cut(s) 2809, 4932
GluI GCNGC 6 cut(s) 459, 2125, 2564, 2780, 3172, 4291
GsaI CCCAGC 1 cut(s) 3869
GsuI CTGGAG 2 cut(s) 1398, 1749
HaeIII GGCC 2 cut(s) 393, 792
HapII CCGG 6 cut(s) 2281, 2711, 2767, 3120, 3168, 4191
HgaI GACGC 1 cut(s) 1079
HhaI GCGC 2 cut(s) 2810, 4933
Hin6I GCGC 2 cut(s) 2808, 4931
HinP1I GCGC 2 cut(s) 2808, 4931
HincII GTYRAC 4 cut(s) 2005, 2985, 3673, 4911
HindII GTYRAC 4 cut(s) 2005, 2985, 3673, 4911
HindIII AAGCTT 4 cut(s) 1820, 1939, 2131, 2735
HpaII CCGG 6 cut(s) 2281, 2711, 2767, 3120, 3168, 4191
HphI GGTGA 4 cut(s) 379, 454, 2934, 4264
Hpy99I CGWCG 3 cut(s) 71, 1073, 1524
HpyCH4III ACNGT 9 cut(s) 2511, 2828, 2989, 3319, 3525, 3898, 4146, 4419, 4937
HpyCH4IV ACGT 9 cut(s) 62, 69, 155, 730, 838, 1549, 2998, 4481, 4907
HpyF10VI GCNNNNNNNGC 8 cut(s) 1551, 1636, 2130, 2734, 2776, 2816, 4287, 4580
HpySE526I ACGT 9 cut(s) 62, 69, 155, 730, 838, 1549, 2998, 4481, 4907
HspAI GCGC 2 cut(s) 2808, 4931
KpnI GGTACC 1 cut(s) 4981
KroI GCCGGC 1 cut(s) 2766
KroNI GCCGGC 1 cut(s) 2768
Ksp22I TGATCA 2 cut(s) 3, 1926
KspI CCGCGG 1 cut(s) 4127
LguI GCTCTTC 1 cut(s) 1086
LmnI GCTCC 4 cut(s) 984, 2179, 3179, 3767
Lsp1109I GCAGC 6 cut(s) 445, 2111, 2550, 2766, 3183, 4302
MaeI CTAG 6 cut(s) 794, 1391, 2189, 3707, 3774, 4871
MaeII ACGT 9 cut(s) 62, 69, 155, 730, 838, 1549, 2998, 4481, 4907
MaeIII GTNAC 6 cut(s) 1294, 1378, 1453, 1755, 3322, 4646
MfeI CAATTG 6 cut(s) 1016, 1472, 2223, 2515, 2758, 3684
MflI RGATCY 5 cut(s) 355, 581, 2392, 4037, 4894
MhlI GDGCHC 4 cut(s) 291, 736, 4303, 4864
MlyI GAGTC 5 cut(s) 3195, 3800, 4372, 4526, 4646
MmeI TCCRAC 5 cut(s) 1076, 1102, 2157, 3294, 3770
Mph1103I ATGCAT 6 cut(s) 411, 1309, 1485, 1842, 3494, 4737
MroNI GCCGGC 1 cut(s) 2766
MroXI GAANNNNTTC 6 cut(s) 1080, 1103, 1131, 1332, 1515, 3348
MslI CAYNNNNRTG 6 cut(s) 1662, 1904, 2190, 4517, 4857, 4944
MspA1I CMGCKG 1 cut(s) 4126
MspCI CTTAAG 2 cut(s) 2066, 2453
MspI CCGG 6 cut(s) 2281, 2711, 2767, 3120, 3168, 4191
MspR9I CCNGG 4 cut(s) 2500, 4067, 4200, 4488
MunI CAATTG 6 cut(s) 1016, 1472, 2223, 2515, 2758, 3684
Mva1269I GAATGC 6 cut(s) 1103, 1840, 2503, 2621, 2821, 3378
MvaI CCWGG 4 cut(s) 2500, 4067, 4200, 4488
MvnI CGCG 3 cut(s) 1073, 2808, 4126
MwoI GCNNNNNNNGC 8 cut(s) 1551, 1636, 2130, 2734, 2776, 2816, 4287, 4580
NaeI GCCGGC 1 cut(s) 2768
NcoI CCATGG 1 cut(s) 585
NdeI CATATG 2 cut(s) 511, 1030
NgoMIV GCCGGC 1 cut(s) 2766
NmeAIII GCCGAG 1 cut(s) 4386
NmuCI GTSAC 1 cut(s) 4646
NsiI ATGCAT 6 cut(s) 411, 1309, 1485, 1842, 3494, 4737
NspI RCATGY 7 cut(s) 319, 409, 1350, 1778, 1840, 1891, 2167
OliI CACNNNNGTG 1 cut(s) 4857
PaeI GCATGC 3 cut(s) 409, 1840, 1891
PaeR7I CTCGAG 1 cut(s) 1136
PagI TCATGA 2 cut(s) 3919, 4083
PaqCI CACCTGC 1 cut(s) 430
PciI ACATGT 1 cut(s) 315
PciSI GCTCTTC 1 cut(s) 1086
PcsI WCGNNNNNNNCGW 1 cut(s) 1528
PctI GAATGC 6 cut(s) 1103, 1840, 2503, 2621, 2821, 3378
PdiI GCCGGC 1 cut(s) 2768
PdmI GAANNNNTTC 6 cut(s) 1080, 1103, 1131, 1332, 1515, 3348
Pfl23II CGTACG 2 cut(s) 63, 3590
PflMI CCANNNNNTGG 2 cut(s) 2015, 2973
PinAI ACCGGT 1 cut(s) 4190
PkrI GCNGC 6 cut(s) 460, 2126, 2565, 2781, 3173, 4292
PleI GAGTC 5 cut(s) 3195, 3799, 4371, 4526, 4645
PpsI GAGTC 5 cut(s) 3195, 3799, 4371, 4526, 4645
Ppu21I YACGTR 1 cut(s) 731
PpuMI RGGWCCY 2 cut(s) 1932, 1951
PscI ACATGT 1 cut(s) 315
PshBI ATTAAT 1 cut(s) 1710
PsiI TTATAA 1 cut(s) 4704
Psp124BI GAGCTC 1 cut(s) 291
Psp1406I AACGTT 1 cut(s) 4907
Psp5II RGGWCCY 2 cut(s) 1932, 1951
Psp6I CCWGG 4 cut(s) 2498, 4065, 4198, 4486
PspFI CCCAGC 1 cut(s) 3865
PspGI CCWGG 4 cut(s) 2498, 4065, 4198, 4486
PspLI CGTACG 2 cut(s) 63, 3590
PspPI GGNCC 5 cut(s) 790, 1932, 1951, 3499, 3891
PspPPI RGGWCCY 2 cut(s) 1932, 1951
PsrI GAACNNNNNNTAC 2 cut(s) 56, 88
PstI CTGCAG 1 cut(s) 3199
PsuI RGATCY 5 cut(s) 355, 581, 2392, 4037, 4894
RseI CAYNNNNRTG 6 cut(s) 1662, 1904, 2190, 4517, 4857, 4944
SacI GAGCTC 1 cut(s) 291
SacII CCGCGG 1 cut(s) 4127
SapI GCTCTTC 1 cut(s) 1086
SatI GCNGC 6 cut(s) 459, 2125, 2564, 2780, 3172, 4291
Sau96I GGNCC 5 cut(s) 790, 1932, 1951, 3499, 3891
SbfI CCTGCAGG 1 cut(s) 3199
SchI GAGTC 5 cut(s) 3195, 3800, 4372, 4526, 4646
ScrFI CCNGG 4 cut(s) 2500, 4067, 4200, 4488
SdaI CCTGCAGG 1 cut(s) 3199
SduI GDGCHC 4 cut(s) 291, 736, 4303, 4864
SfcI CTRYAG 2 cut(s) 3195, 4441
Sfr274I CTCGAG 1 cut(s) 1136
Sfr303I CCGCGG 1 cut(s) 4127
SgrBI CCGCGG 1 cut(s) 4127
SinI GGWCC 4 cut(s) 1932, 1951, 3499, 3891
SlaI CTCGAG 1 cut(s) 1136
SmiMI CAYNNNNRTG 6 cut(s) 1662, 1904, 2190, 4517, 4857, 4944
SmlI CTYRAG 9 cut(s) 176, 468, 574, 1136, 2066, 2128, 2453, 3327, 3812
SmoI CTYRAG 9 cut(s) 176, 468, 574, 1136, 2066, 2128, 2453, 3327, 3812
SphI GCATGC 3 cut(s) 409, 1840, 1891
Sse8387I CCTGCAGG 1 cut(s) 3199
SsiI CCGC 6 cut(s) 1073, 1879, 2568, 4124, 4126, 4405
SspI AATATT 4 cut(s) 859, 2930, 4173, 4678
SspMI CTAG 6 cut(s) 794, 1391, 2189, 3707, 3774, 4871
SstI GAGCTC 1 cut(s) 291
StyD4I CCNGG 4 cut(s) 2498, 4065, 4198, 4486
StyI CCWWGG 3 cut(s) 585, 2992, 4864
TaaI ACNGT 9 cut(s) 2511, 2828, 2989, 3319, 3525, 3898, 4146, 4419, 4937
TaiI ACGT 9 cut(s) 65, 72, 158, 733, 841, 1552, 3001, 4484, 4910
TatI WGTACW 3 cut(s) 1985, 2828, 4236
TscAI CASTG 9 cut(s) 1620, 2514, 2617, 2841, 4137, 4283, 4581, 4940, 4949
TseFI GTSAC 1 cut(s) 4646
TseI GCWGC 6 cut(s) 458, 2124, 2563, 2779, 3171, 4290
Tsp45I GTSAC 1 cut(s) 4646
TspGWI ACGGA 3 cut(s) 3166, 4617, 4688
TspRI CASTG 9 cut(s) 1620, 2514, 2617, 2841, 4137, 4283, 4581, 4940, 4949
Van91I CCANNNNNTGG 2 cut(s) 2015, 2973
Vha464I CTTAAG 2 cut(s) 2066, 2453
VpaK11BI GGWCC 4 cut(s) 1932, 1951, 3499, 3891
VspI ATTAAT 1 cut(s) 1710
XapI RAATTY 8 cut(s) 386, 1633, 2254, 3138, 4215, 4370, 4497, 4848
XbaI TCTAGA 1 cut(s) 2188
XceI RCATGY 7 cut(s) 319, 409, 1350, 1778, 1840, 1891, 2167
XhoI CTCGAG 1 cut(s) 1136
XmiI GTMKAC 2 cut(s) 3520, 4640
XmnI GAANNNNTTC 6 cut(s) 1080, 1103, 1131, 1332, 1515, 3348
XspI CTAG 6 cut(s) 794, 1391, 2189, 3707, 3774, 4871
Zsp2I ATGCAT 6 cut(s) 411, 1309, 1485, 1842, 3494, 4737
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.