FvH4_5g38561

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000129
Physical Location & Seq
Forward (+)
28507263 .. 28521388
14126 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g38561.t3

Sequence Viewer

Length: 5034 bp
ATGCTCAGATTTCTCACACAGTTCGAACAAGTGATGGCCTCCTACCCAGTCCGCCTTGTCCTCTTGTCTTGTATTTGCCCTCTGGTTTTCTTCTCTGCTTTGTTTCTCTTCAGCTTTATCTCTTCCGGGTTGTACCTCTTCTTCTTCTTCTCCATTGTTTCTATTTGCTGCTGCCTTTTGGCTTTCACTCTTTCCCATAAACAGACACCCGCAACACCAGACACTTCTGCTTCAGCTACCACCAACACAGAAGTGATTCCTTCTTCTTCGTCGTCGTCTTCTTCTCCACGGTCCGGTAAACTCACCTACGATGTGTTCCTGAGTTTCAGAGGTGAAGATACCCGCAAGAATTTCACGGACCATCTCTACACTGCTCTCTGGCAGAAAGGGATACACACGTTTCGAGATGATGAAGAGCTTGTGAGGGGGGAATCGATTGCTCCCAATCTCTTGAAAGCAATCGAAGAATCAAGATATGTCATTGTTGTCTTCTCACCAAACTACGCCGATTCTGCATGGTGTTTGGATGAGGTTGCCGAGGTCGCCGATTGCATGAAGGAAATGGGAACAAAAGTCCTTCCAGTGTTCTACCATGTTGATCCGTCGGAGGTACGAAGACAAACAGGAGAGCACTTTGGGAAAGCATTTGAGAAGCATCAGAAGAGGTATAAAAAGGAACCAAACAAAGTACAGAGGTGGAAAGACGCTCTTTTCCAAGTAGGCAATCTCTCTGGATGGCACCTGCAAGATGGGTCTGAATCCAAAGCAATTCAAGAAATTGTAGAAAAGCTTTTCATTGAGTTGAATAAAATTATCTCCACATCTGAGGGATTAGTTGGGATGGATTCTCACCTGAATGAATTGTTTCCCTACTTAGACATTGGGGGTCTCGATGTTCGCATCATAGGGATCTGTGGAATGGGTGGTATCGGGAAAACTACTATTGCACAAGTGGTTTTTGAAAGGATAAGAGCTCAATTTGAAGGTGATAGCTTTCTTGAGAATGTTAAACAGGAAGCTGAAAAGCAGCGTAGTACAATTCATTTACAAGAGAAACTTCTCTTCAACTTGCTGAATAGTAATGTCAATGTACAGAACACTAAAATGGGAAAAGACATTATTAAGCATAGACTATCAACTAAAAGGGTGCTTGTTGTTCTTGATGATGTGGATCAATATAAACAATTGGAAACATTGTGTCACCGTACTTGGTTTGGTCCGGGTAGTAGAATCATCATAACATCCAGAGATGAACACTTGATCAGTGCATTTGGAGCAGATAAAGTATACATGGTGAATGCATTAACTGATACTGAAGCTTTTGATCTCTTTTGTTTGAAAGCCTTCAATGAAGACGAGATTGGAGAAGATTTTCTTAAGCTATCCAATGAATTTTTGAAATATGCTAATGGCCTTCCATTAGCTATTAAAGTTTTGGGATCATCTGTTCGTGGTAGAAATGTAAAGTTAATGTCAAGTGCATTGAAGAGGCTTGAAAAAAATCCTCCAAAAGGAATTATTGATGTTCTTAAAGTTAGTTATGATGGATTAGAAGAAACAGAAAAGAAAACTTTTTTAGACATCACGTGTTTCTTTAAAGGAGTGAACAAGGATCGTGTTATAAGAATACTAAATGGTTGTGGCGATGGTGTGGAGATGGATGTACAAGTTCTAATTGAAAGATCTTTGGTAACTCTATTTGGAACAAAATTGTGGATGCACGATTTGATACAAGAAATGGGTCGGGAAGTTGTTTTTCAAGAATGCCGTGAAGAGCCTGGAAAACAAAGCAGGTTGTGGCTTCCCAAAGATATCATACACGTACTTGGTCAGAGTAAGGCGACGAGTGCAGTTCAAAGCATATACCTCGAATGTTCAACAGGAGACAATGTTGTTCACTCCATTGATAATGCCTTCTCAAATATGGACAGATTGAGATTGCTCAAGATTTGGAATTTGAAGTGTTCCGGAAACATCAGATATATTTCTAATAAGTTACAGTATCTTGAATGGCATGAGTGTCCTTTGGATTCTTTCCCATCAGACTTTCAACCGGAGAAACTTGTTGAAGTTCACATGCCTTTTAGCCGCATCATACAACTATGGAGGGGAACACAAGGATGGAGCATGCTACGGCATTTAGATCTGAGTGGTTCACCATACTTGATATCAACTCCTGACTTCACTGAGGTTCCTTATCTTGAAGAGTTGCTGTTTGAGAGTTGCACTGGATTGGTGGTGGTCCACCCTTCCCTTGGATTTCTGAAGAAACTTCGTCATTTGGATTTGCAATTTTGCAAGTCTATTGAGAGCCTTCCACCTCTCACTGCCTTGGAATCCCTTCAAATATTGAAGCTTTCATTCTGTTCCGGACTGAAGAAGTTTCCAGAAATTGAAGGAAATATGAAAAGCTTGCTGGAGCTTCATTTGGATGGAACCAGCATTGAGGAATTGCCTCCATCAATTGAACATTTGACTGCTCTTACCATGTTGAATCTATCAAACTGTGGGAACCTTTTGCATCTTCCTGACACCATTGGGTGCTTGAAATCGTTAAAAAGTCTATCTCTAAAAGGTTGCTCCAAAATTGATGAGATACCTGAGAATCTGAATGATATGAAATGTCTGGAGAAGCTTTTCATTGGTGAAACTTCCATAAGAGAGTTATCTATCATCGGAGGCATGAAGAATCTACAGTATCTTTCCTGCAGAGGATGTAAATGTCTAGTTTTAGAATCATACAAAAGTCTGGCTTTGTTATCAAGTTTGATAGAGCTAGATCTGAGTTATTGCGATCTGATGGATGGAGCATTTCTCAATGGTTTTAGCAGCCTGATCTCCTTACGAGTGTTAGAACTAGGTGGTAATTGTTTCGTGCGATTACCTGAAAGTATCTCTCAGCTCTCAAAGCTCGAGACTCTGGACTTGAGTGGTTGCAGACAGCTTCAATTGCTGCCCAATAAGTTTCCGTTAAGTCTTGATCACGTGTATGCACACAATTGTACTTCACTGAGAGATTACCCAAATCAAATCAAAGTATTGTATTCATCAGAGTCAGGTGTGACTATGGTCAATTCCCTCGATTCTTCAGCACCTAGAGTCTCACAAAGTTCCACAAGCTTTACTTTTCCAAGAAATGCGGGGGAATCATTACAACTGCTTGTGACACATAAGCATACAGAGGGGAAACAAGTTGATCATGTGCCACTGACAAATTTGAATCAAGTGACAAATTTTGCACCAGATTTCTACAGCGCAAATATTAGCGATCAAATTCCAGAGTGGTTCAGCACTATATCTACTAGAAATCCCATATCGATCCCGATAGCTCAAAATCTGGCGGATGATAACAAGTGGAAGGGATTTGCGGCGTGTGCTATATTTGCAGTCAAGGGGCATACTGAACTCTCCCATATTGAACCAGATCCTGATTTTTCTAATTACTCGTATCAAATCATATTGGAAACTGATGTTCTGCGTCTGGAACAACATGTGTTGAAACTGGATTCAGGTCACTTTGGATCAAGTTCTCATCTGCTTGTGATGTTCTGTATATCTAGTTTGAGGTTTCCACAGTGGGGGTTAAATCAATCCACTGTGGTGAGGGCTATATTCGAAACCACCAATCCATCCATGGTGGTCCAGAAATGTGGAATTCGCCTAGTCTATGAGAAAGATGATGGATGGTATTCCAGAATATCCGGGTGGAAGGATGAGCCAATTTGGCTTAATCAAGTTGAATTCGACTCTGTACGGGAATCTGACTGGCTTAGGCAATCTTTGAAACGGGAGAAGTTCATTCCACCCTTTGAAGAAGAATCTACACTTGTGCTGCGAAAGAACCTCGAGTCTGTTCTTCCAAGATACCTTGAGGCATTGAACAGTTATTCTAAAATATACAAATTTAACCTCAGAGGAAGTCCAGGGTGGTTTCACAAGTGGTTTACCCATGGTACTGGTAGGAGGTCAAACTTGACCAGAGCGTTAATTGAGCTGCCTCTGAAACTACATAAGAATAAGAAGTGGATGGGATTTGCAATATTTGCATCACTTGTGGAGGAGGTGGGGCAGACAAAGAAAGAGGATGACTATGGTGTATCATTTACGCTGACAACTTGTGATGTATCTGCTCCAGTGTGCAGAGAACTTAAGCACGTCCGGCCATTGTCAGAAGAACATCATCAACTCTTGGTTGCTTACATACCGCGGGGACAGATACCCGAAGAGTTGCTCATTCAGACATTAACAACTGCAATGGAATTTGACATCAGGATCGATAGCCCTCATGTAAAGGTTCAAAGATGTGGGTTCCGTATTGTGTACCAGGAAGATACACAAGGCTTTGCTGACACAATTATCCGGTGCATGCAGAGGGAAAATTCTCTTGAATCTCACAATATGTTGGTGGTAGAAGAATGGATGCAGTTGTTACGATTACAAGGTGCCTATTTCGAGAGAAGGAAAGAACGGAAAAAACAACTTGAGTTGCACTCACATATACCTATATATAGAAATTGGGAATGGTCTGACCCTCGTTGTGTATATTGTGTCTTTGAGAGAGTTGAAATCTCCAAGTTGAAATGGTTCATGTCTTTCATCAACCAAGGCAACTCTGCAGAAATCCAACTGCCTCAGAATTTGTTTGATGATGATAATTGGTTGGGTTTTGCTGTATGTTTCCAGTTGTCCCCCGGCCAATATCCAAACATTAGTCTTGGCAGTAGCGCTCCTGATTCAGAGGATGTTGTACTTAGTTACTGTCTGGATTCTGATGTTGCTTGGAAGAGTTTATTTGATTTCAAAATGAATCTAAGTTTAAAGATTCGAGAGCCATCGACATGCGATATGACTTGGATCCATCACATACCACGCGCTAAATGTCGTAGTAATATGTGGAGGCAATGCAAATTGGCCAGGATAACTATCTGGTTGTCTAACCCATGCTTAGCGGTGCATAATTGTGCCCTTCGTCTGCTATTGAAGGAGGACGTTGAACATCTTCTAGAAACTCTGACCCTCGCCGAATCACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1678

Amino Acids

191.15

Weight (kDa)

6.14

Isoelectric Point (pI)

45.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 2 - 90 1.4e-15 TIR domain
NB-ARC PF00931 114 - 267 7.4e-24 NB-ARC domain
WHD_ROQ1 PF23282 334 - 403 4.4e-13 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 577 - 740 9.9e-12 Leucine-rich repeat region
LRR_13 PF23286 595 - 682 8.5e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 734 - 842 1.5e-06 Leucine-rich repeat region
C-JID PF20160 883 - 1016 9.5e-06 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1622
AarI CACCTGC 1 cut(s) 750
Acc36I ACCTGC 2 cut(s) 750, 1782
AccB1I GGYRCC 2 cut(s) 738, 4442
AccI GTMKAC 1 cut(s) 1287
AccII CGCG 2 cut(s) 4207, 4872
AccIII TCCGGA 2 cut(s) 1967, 2369
AcoI YGGCCR 3 cut(s) 4160, 4693, 4911
AcuI CTGAAG 6 cut(s) 94, 216, 1335, 2285, 2396, 3072
AcvI CACGTG 2 cut(s) 1587, 2986
AfeI AGCGCT 1 cut(s) 4726
AfiI CCNNNNNNNGG 4 cut(s) 293, 1511, 2255, 3578
AflII CTTAAG 2 cut(s) 1376, 4148
AflIII ACRYGT 5 cut(s) 396, 1586, 1819, 2985, 3490
AjiI CACGTC 1 cut(s) 4156
AjnI CCWGG 4 cut(s) 1777, 3922, 4323, 4913
AjuI GAANNNNNNNTTGG 2 cut(s) 1344, 1376
AleI CACNNNNGTG 2 cut(s) 251, 3599
AloI GAACNNNNNNTCC 2 cut(s) 1431, 1463
Alw21I GWGCWC 2 cut(s) 633, 976
Alw26I GTCTC 4 cut(s) 893, 1878, 2909, 3106
AlwNI CAGNNNCTG 1 cut(s) 3428
Ama87I CYCGRG 2 cut(s) 2912, 3845
Aor13HI TCCGGA 2 cut(s) 1967, 2369
Aor51HI AGCGCT 1 cut(s) 4726
AoxI GGCC 5 cut(s) 36, 1411, 4160, 4693, 4911
ApeKI GCWGC 7 cut(s) 168, 171, 1027, 2829, 2953, 3832, 3994
Asp700I GAANNNNTTC 8 cut(s) 255, 864, 1343, 1371, 2340, 2636, 4583, 4998
AspLEI GCGC 3 cut(s) 3257, 4727, 4874
AspS9I GGNCC 5 cut(s) 291, 358, 1217, 2242, 3640
AsuC2I CCSGG 4 cut(s) 127, 1221, 3703, 4692
AsuII TTCGAA 2 cut(s) 24, 3615
AvaI CYCGRG 2 cut(s) 2912, 3845
AvaII GGWCC 5 cut(s) 291, 358, 1217, 2242, 3640
BaeGI GKGCMC 1 cut(s) 4966
BaeI ACNNNNGTAYC 2 cut(s) 383, 416
BalI TGGCCA 1 cut(s) 4913
BamHI GGATCC 1 cut(s) 4854
BanI GGYRCC 2 cut(s) 738, 4442
BanII GRGCYC 1 cut(s) 976
BarI GAAGNNNNNNTAC 2 cut(s) 125, 157
BbrPI CACGTG 2 cut(s) 1587, 2986
BbsI GAAGAC 4 cut(s) 270, 481, 622, 1359
Bbv12I GWGCWC 2 cut(s) 633, 976
BbvI GCAGC 7 cut(s) 155, 158, 1039, 2841, 2940, 3819, 3981
BceAI ACGGC 2 cut(s) 1752, 2150
BcgI CGANNNNNNTGC 2 cut(s) 1849, 1883
BciT130I CCWGG 4 cut(s) 1779, 3924, 4325, 4915
BciVI GTATCC 1 cut(s) 384
BclI TGATCA 3 cut(s) 1260, 2980, 3196
BcnI CCSGG 4 cut(s) 127, 1221, 3703, 4692
BcoDI GTCTC 4 cut(s) 893, 1878, 2909, 3106
BfaI CTAG 8 cut(s) 2726, 2777, 2858, 3096, 3303, 3558, 3662, 5003
BfmI CTRYAG 4 cut(s) 2693, 2707, 3250, 4614
BfoI RGCGCY 1 cut(s) 4728
BfrI CTTAAG 2 cut(s) 1376, 4148
BfuAI ACCTGC 2 cut(s) 750, 1782
BfuI GTATCC 1 cut(s) 384
BglI GCCNNNNNGGC 1 cut(s) 3724
BglII AGATCT 3 cut(s) 1682, 2143, 2779
BisI GCNGC 9 cut(s) 169, 172, 1028, 2089, 2830, 2954, 3369, 3833, 3995
BlpI GCTNAGC 1 cut(s) 4945
BlsI GCNGC 9 cut(s) 170, 173, 1029, 2090, 2831, 2955, 3370, 3834, 3996
Bme1390I CCNGG 8 cut(s) 127, 1221, 1779, 3703, 3924, 4325, 4692, 4915
Bme18I GGWCC 5 cut(s) 291, 358, 1217, 2242, 3640
BmeT110I CYCGRG 2 cut(s) 2912, 3845
BmgBI CACGTC 1 cut(s) 4156
BmgT120I GGNCC 5 cut(s) 291, 358, 1217, 2242, 3640
BmiI GGNNCC 8 cut(s) 678, 740, 2193, 2437, 2513, 4310, 4444, 4856
BmrFI CCNGG 8 cut(s) 127, 1221, 1779, 3703, 3924, 4325, 4692, 4915
BmrI ACTGGG 1 cut(s) 41
BmsI GCATC 7 cut(s) 664, 909, 1707, 2100, 2530, 4055, 4410
BmuI ACTGGG 1 cut(s) 41
BoxI GACNNNNGTC 1 cut(s) 3068
BpiI GAAGAC 4 cut(s) 270, 481, 622, 1359
BplI GAGNNNNNCTC 4 cut(s) 2799, 2831, 4475, 4507
BpmI CTGGAG 3 cut(s) 2438, 2648, 4116
Bpu10I CCTNAGC 1 cut(s) 3770
Bpu1102I GCTNAGC 1 cut(s) 4945
Bpu14I TTCGAA 2 cut(s) 24, 3615
BpuEI CTTGAG 5 cut(s) 1019, 1928, 2947, 3890, 4502
BpuMI CCSGG 4 cut(s) 127, 1221, 3703, 4692
Bsa29I ATCGAT 3 cut(s) 434, 3317, 4275
BsaAI YACGTR 3 cut(s) 1587, 1822, 2986
BsaBI GATNNNNATC 2 cut(s) 1170, 4922
BsaI GGTCTC 1 cut(s) 893
BsaWI WCCGGW 5 cut(s) 293, 1967, 2053, 2369, 4359
BsaXI ACNNNNNCTCC 2 cut(s) 1875, 1905
Bsc4I CCNNNNNNNGG 4 cut(s) 293, 1511, 2255, 3578
Bse1I ACTGG 8 cut(s) 47, 581, 2233, 3507, 3770, 3961, 4133, 4681
Bse3DI GCAATG 2 cut(s) 4260, 4907
Bse8I GATNNNNATC 2 cut(s) 1170, 4922
BseAI TCCGGA 2 cut(s) 1967, 2369
BseBI CCWGG 4 cut(s) 1779, 3924, 4325, 4915
BseCI ATCGAT 3 cut(s) 434, 3317, 4275
BseJI GATNNNNATC 2 cut(s) 1170, 4922
BseLI CCNNNNNNNGG 4 cut(s) 293, 1511, 2255, 3578
BseMI GCAATG 2 cut(s) 4260, 4907
BseNI ACTGG 8 cut(s) 47, 581, 2233, 3507, 3770, 3961, 4133, 4681
BseRI GAGGAG 1 cut(s) 4073
BseSI GKGCMC 1 cut(s) 4966
BseXI GCAGC 7 cut(s) 155, 158, 1039, 2841, 2940, 3819, 3981
BsgI GTGCAG 2 cut(s) 1869, 4159
Bsh1236I CGCG 2 cut(s) 4207, 4872
BshFI GGCC 5 cut(s) 38, 1413, 4162, 4695, 4913
BshNI GGYRCC 2 cut(s) 738, 4442
BshVI ATCGAT 3 cut(s) 434, 3317, 4275
BsiHKAI GWGCWC 2 cut(s) 633, 976
BsiHKCI CYCGRG 2 cut(s) 2912, 3845
BslFI GGGAC 2 cut(s) 4224, 4672
BslI CCNNNNNNNGG 4 cut(s) 293, 1511, 2255, 3578
BsmAI GTCTC 4 cut(s) 893, 1878, 2909, 3106
BsmFI GGGAC 2 cut(s) 4224, 4672
BsmI GAATGC 2 cut(s) 1303, 1769
BsnI GGCC 5 cut(s) 38, 1413, 4162, 4695, 4913
Bso31I GGTCTC 1 cut(s) 893
BsoBI CYCGRG 2 cut(s) 2912, 3845
Bsp119I TTCGAA 2 cut(s) 24, 3615
Bsp1286I GDGCHC 3 cut(s) 633, 976, 4966
Bsp13I TCCGGA 2 cut(s) 1967, 2369
Bsp1407I TGTACA 2 cut(s) 1090, 1663
Bsp1720I GCTNAGC 1 cut(s) 4945
Bsp19I CCATGG 2 cut(s) 3633, 3949
BspANI GGCC 5 cut(s) 38, 1413, 4162, 4695, 4913
BspDI ATCGAT 3 cut(s) 434, 3317, 4275
BspEI TCCGGA 2 cut(s) 1967, 2369
BspFNI CGCG 2 cut(s) 4207, 4872
BspLI GGNNCC 8 cut(s) 678, 740, 2193, 2437, 2513, 4310, 4444, 4856
BspMAI CTGCAG 2 cut(s) 2711, 4618
BspMI ACCTGC 2 cut(s) 750, 1782
BspQI GCTCTTC 2 cut(s) 408, 1767
BspT104I TTCGAA 2 cut(s) 24, 3615
BspT107I GGYRCC 2 cut(s) 738, 4442
BspTI CTTAAG 2 cut(s) 1376, 4148
BspTNI GGTCTC 1 cut(s) 893
BsrDI GCAATG 2 cut(s) 4260, 4907
BsrGI TGTACA 2 cut(s) 1090, 1663
BsrI ACTGG 8 cut(s) 47, 581, 2233, 3507, 3770, 3961, 4133, 4681
BssNAI GTATAC 1 cut(s) 1288
BssT1I CCWWGG 5 cut(s) 2254, 2331, 3633, 3949, 4603
Bst1107I GTATAC 1 cut(s) 1288
Bst2UI CCWGG 4 cut(s) 1779, 3924, 4325, 4915
BstAFI CTTAAG 2 cut(s) 1376, 4148
BstAPI GCANNNNNTGC 1 cut(s) 4043
BstAUI TGTACA 2 cut(s) 1090, 1663
BstBAI YACGTR 3 cut(s) 1587, 1822, 2986
BstBI TTCGAA 2 cut(s) 24, 3615
BstC8I GCNNGC 3 cut(s) 2129, 2414, 4367
BstDSI CCRYGG 4 cut(s) 287, 3633, 3949, 4205
BstENI CCTNNNNNAGG 1 cut(s) 1509
BstFNI CGCG 2 cut(s) 4207, 4872
BstH2I RGCGCY 1 cut(s) 4728
BstHHI GCGC 3 cut(s) 3257, 4727, 4874
BstMAI GTCTC 4 cut(s) 893, 1878, 2909, 3106
BstNI CCWGG 4 cut(s) 1779, 3924, 4325, 4915
BstNSI RCATGY 5 cut(s) 2080, 2131, 3494, 4369, 4842
BstPAI GACNNNNGTC 1 cut(s) 3068
BstSCI CCNGG 8 cut(s) 125, 1219, 1777, 3701, 3922, 4323, 4690, 4913
BstSFI CTRYAG 4 cut(s) 2693, 2707, 3250, 4614
BstSLI GKGCMC 1 cut(s) 4966
BstUI CGCG 2 cut(s) 4207, 4872
BstV1I GCAGC 7 cut(s) 155, 158, 1039, 2841, 2940, 3819, 3981
BstV2I GAAGAC 4 cut(s) 270, 481, 622, 1359
BstX2I RGATCY 6 cut(s) 909, 1682, 2143, 2779, 3424, 4854
BstXI CCANNNNNNTGG 2 cut(s) 3650, 3956
BstYI RGATCY 6 cut(s) 909, 1682, 2143, 2779, 3424, 4854
BstZ17I GTATAC 1 cut(s) 1288
Bsu15I ATCGAT 3 cut(s) 434, 3317, 4275
BsuI GTATCC 1 cut(s) 384
BsuRI GGCC 5 cut(s) 38, 1413, 4162, 4695, 4913
BsuTUI ATCGAT 3 cut(s) 434, 3317, 4275
BtgI CCRYGG 4 cut(s) 287, 3633, 3949, 4205
BtgZI GCGATG 1 cut(s) 1659
BtrI CACGTC 1 cut(s) 4156
BtsI GCAGTG 2 cut(s) 369, 2325
BveI ACCTGC 2 cut(s) 750, 1782
Cac8I GCNNGC 3 cut(s) 2129, 2414, 4367
CaiI CAGNNNCTG 1 cut(s) 3428
CfoI GCGC 3 cut(s) 3257, 4727, 4874
Cfr13I GGNCC 5 cut(s) 291, 358, 1217, 2242, 3640
Cfr42I CCGCGG 1 cut(s) 4208
ClaI ATCGAT 3 cut(s) 434, 3317, 4275
CpoI CGGWCCG 1 cut(s) 291
CseI GACGC 2 cut(s) 713, 3467
CspI CGGWCCG 1 cut(s) 291
DraI TTTAAA 2 cut(s) 1597, 4818
EaeI YGGCCR 3 cut(s) 4160, 4693, 4911
EciI GGCGGA 2 cut(s) 41, 3356
Ecl136II GAGCTC 1 cut(s) 974
Eco130I CCWWGG 5 cut(s) 2254, 2331, 3633, 3949, 4603
Eco24I GRGCYC 1 cut(s) 976
Eco31I GGTCTC 1 cut(s) 893
Eco32I GATATC 2 cut(s) 1813, 2169
Eco47I GGWCC 5 cut(s) 291, 358, 1217, 2242, 3640
Eco47III AGCGCT 1 cut(s) 4726
Eco53kI GAGCTC 1 cut(s) 974
Eco57I CTGAAG 6 cut(s) 94, 216, 1335, 2285, 2396, 3072
Eco72I CACGTG 2 cut(s) 1587, 2986
Eco88I CYCGRG 2 cut(s) 2912, 3845
EcoICRI GAGCTC 1 cut(s) 974
EcoNI CCTNNNNNAGG 1 cut(s) 1509
EcoRI GAATTC 2 cut(s) 3654, 3740
EcoRII CCWGG 4 cut(s) 1777, 3922, 4323, 4913
EcoRV GATATC 2 cut(s) 1813, 2169
EcoT14I CCWWGG 5 cut(s) 2254, 2331, 3633, 3949, 4603
EcoT22I ATGCAT 1 cut(s) 1303
EcoT38I GRGCYC 1 cut(s) 976
ErhI CCWWGG 5 cut(s) 2254, 2331, 3633, 3949, 4603
FaqI GGGAC 2 cut(s) 4224, 4672
FauI CCCGC 4 cut(s) 217, 350, 3133, 4200
FbaI TGATCA 3 cut(s) 1260, 2980, 3196
FblI GTMKAC 1 cut(s) 1287
Fnu4HI GCNGC 9 cut(s) 169, 172, 1028, 2089, 2830, 2954, 3369, 3833, 3995
FriOI GRGCYC 1 cut(s) 976
Fsp4HI GCNGC 9 cut(s) 169, 172, 1028, 2089, 2830, 2954, 3369, 3833, 3995
FspBI CTAG 8 cut(s) 2726, 2777, 2858, 3096, 3303, 3558, 3662, 5003
GlaI GCGC 3 cut(s) 3256, 4726, 4873
GluI GCNGC 9 cut(s) 169, 172, 1028, 2089, 2830, 2954, 3369, 3833, 3995
GsuI CTGGAG 3 cut(s) 2438, 2648, 4116
HaeII RGCGCY 1 cut(s) 4728
HaeIII GGCC 5 cut(s) 38, 1413, 4162, 4695, 4913
HgaI GACGC 2 cut(s) 713, 3467
HhaI GCGC 3 cut(s) 3257, 4727, 4874
Hin6I GCGC 3 cut(s) 3255, 4725, 4872
HinP1I GCGC 3 cut(s) 3255, 4725, 4872
HindIII AAGCTT 6 cut(s) 788, 1317, 2354, 2410, 2633, 3118
Hpy166II GTNNAC 9 cut(s) 299, 1288, 1606, 1897, 2074, 2156, 2245, 3945, 4321
Hpy8I GTNNAC 9 cut(s) 299, 1288, 1606, 1897, 2074, 2156, 2245, 3945, 4321
Hpy99I CGWCG 4 cut(s) 274, 277, 607, 1846
HpyCH4IV ACGT 6 cut(s) 398, 1586, 1821, 2985, 4155, 4989
HpySE526I ACGT 6 cut(s) 398, 1586, 1821, 2985, 4155, 4989
HspAI GCGC 3 cut(s) 3255, 4725, 4872
Kpn2I TCCGGA 2 cut(s) 1967, 2369
Ksp22I TGATCA 3 cut(s) 1260, 2980, 3196
KspI CCGCGG 1 cut(s) 4208
LguI GCTCTTC 2 cut(s) 408, 1767
LmnI GCTCC 8 cut(s) 445, 1274, 2124, 2419, 2585, 2807, 4135, 4732
Lsp1109I GCAGC 7 cut(s) 155, 158, 1039, 2841, 2940, 3819, 3981
LweI GCATC 7 cut(s) 664, 909, 1707, 2100, 2530, 4055, 4410
MaeI CTAG 8 cut(s) 2726, 2777, 2858, 3096, 3303, 3558, 3662, 5003
MaeII ACGT 6 cut(s) 398, 1586, 1821, 2985, 4155, 4989
MaeIII GTNAC 9 cut(s) 1199, 1690, 1995, 3061, 3163, 3226, 3512, 4428, 4754
MfeI CAATTG 4 cut(s) 1184, 2463, 2948, 2998
MflI RGATCY 6 cut(s) 909, 1682, 2143, 2779, 3424, 4854
MhlI GDGCHC 3 cut(s) 633, 976, 4966
MlsI TGGCCA 1 cut(s) 4913
MluNI TGGCCA 1 cut(s) 4913
MlyI GAGTC 5 cut(s) 2911, 3062, 3108, 3740, 3857
MmeI TCCRAC 2 cut(s) 585, 4648
Mox20I TGGCCA 1 cut(s) 4913
Mph1103I ATGCAT 1 cut(s) 1303
MroI TCCGGA 2 cut(s) 1967, 2369
MroXI GAANNNNTTC 8 cut(s) 255, 864, 1343, 1371, 2340, 2636, 4583, 4998
MscI TGGCCA 1 cut(s) 4913
MslI CAYNNNNRTG 9 cut(s) 251, 855, 1103, 2119, 2430, 2988, 3599, 4837, 4959
Msp20I TGGCCA 1 cut(s) 4913
MspA1I CMGCKG 1 cut(s) 4207
MspCI CTTAAG 2 cut(s) 1376, 4148
MspR9I CCNGG 8 cut(s) 127, 1221, 1779, 3703, 3924, 4325, 4692, 4915
MunI CAATTG 4 cut(s) 1184, 2463, 2948, 2998
Mva1269I GAATGC 2 cut(s) 1303, 1769
MvaI CCWGG 4 cut(s) 1779, 3924, 4325, 4915
MvnI CGCG 2 cut(s) 4207, 4872
NciI CCSGG 4 cut(s) 127, 1221, 3703, 4692
NcoI CCATGG 2 cut(s) 3633, 3949
NlaIV GGNNCC 8 cut(s) 678, 740, 2193, 2437, 2513, 4310, 4444, 4856
NmeAIII GCCGAG 1 cut(s) 562
NmuCI GTSAC 5 cut(s) 1199, 3061, 3163, 3226, 3512
NsiI ATGCAT 1 cut(s) 1303
NspI RCATGY 5 cut(s) 2080, 2131, 3494, 4369, 4842
NspV TTCGAA 2 cut(s) 24, 3615
OliI CACNNNNGTG 2 cut(s) 251, 3599
PaeI GCATGC 2 cut(s) 2131, 4369
PaeR7I CTCGAG 2 cut(s) 2912, 3845
PaqCI CACCTGC 1 cut(s) 750
PciI ACATGT 1 cut(s) 3490
PciSI GCTCTTC 2 cut(s) 408, 1767
PctI GAATGC 2 cut(s) 1303, 1769
PdmI GAANNNNTTC 8 cut(s) 255, 864, 1343, 1371, 2340, 2636, 4583, 4998
PkrI GCNGC 9 cut(s) 170, 173, 1029, 2090, 2831, 2955, 3370, 3834, 3996
PleI GAGTC 5 cut(s) 2911, 3061, 3107, 3740, 3856
PmaCI CACGTG 2 cut(s) 1587, 2986
PmlI CACGTG 2 cut(s) 1587, 2986
PpsI GAGTC 5 cut(s) 2911, 3061, 3107, 3740, 3856
Ppu21I YACGTR 3 cut(s) 1587, 1822, 2986
PscI ACATGT 1 cut(s) 3490
PshAI GACNNNNGTC 1 cut(s) 3068
PsiI TTATAA 1 cut(s) 1622
Psp124BI GAGCTC 1 cut(s) 976
Psp6I CCWGG 4 cut(s) 1777, 3922, 4323, 4913
PspCI CACGTG 2 cut(s) 1587, 2986
PspGI CCWGG 4 cut(s) 1777, 3922, 4323, 4913
PspN4I GGNNCC 8 cut(s) 678, 740, 2193, 2437, 2513, 4310, 4444, 4856
PspPI GGNCC 5 cut(s) 291, 358, 1217, 2242, 3640
PspXI VCTCGAGB 1 cut(s) 3845
PstI CTGCAG 2 cut(s) 2711, 4618
PstNI CAGNNNCTG 1 cut(s) 3428
PsuI RGATCY 6 cut(s) 909, 1682, 2143, 2779, 3424, 4854
RseI CAYNNNNRTG 9 cut(s) 251, 855, 1103, 2119, 2430, 2988, 3599, 4837, 4959
Rsr2I CGGWCCG 1 cut(s) 291
RsrII CGGWCCG 1 cut(s) 291
SacI GAGCTC 1 cut(s) 976
SacII CCGCGG 1 cut(s) 4208
SapI GCTCTTC 2 cut(s) 408, 1767
SatI GCNGC 9 cut(s) 169, 172, 1028, 2089, 2830, 2954, 3369, 3833, 3995
Sau96I GGNCC 5 cut(s) 291, 358, 1217, 2242, 3640
SchI GAGTC 5 cut(s) 2911, 3062, 3108, 3740, 3857
ScrFI CCNGG 8 cut(s) 127, 1221, 1779, 3703, 3924, 4325, 4692, 4915
SduI GDGCHC 3 cut(s) 633, 976, 4966
SfaNI GCATC 7 cut(s) 664, 909, 1707, 2100, 2530, 4055, 4410
SfcI CTRYAG 4 cut(s) 2693, 2707, 3250, 4614
Sfr274I CTCGAG 2 cut(s) 2912, 3845
Sfr303I CCGCGG 1 cut(s) 4208
SfuI TTCGAA 2 cut(s) 24, 3615
SgrBI CCGCGG 1 cut(s) 4208
SinI GGWCC 5 cut(s) 291, 358, 1217, 2242, 3640
SlaI CTCGAG 2 cut(s) 2912, 3845
SmiMI CAYNNNNRTG 9 cut(s) 251, 855, 1103, 2119, 2430, 2988, 3599, 4837, 4959
SmlI CTYRAG 9 cut(s) 998, 1376, 1943, 2912, 2926, 3845, 3869, 4148, 4481
SmoI CTYRAG 9 cut(s) 998, 1376, 1943, 2912, 2926, 3845, 3869, 4148, 4481
SphI GCATGC 2 cut(s) 2131, 4369
SspI AATATT 3 cut(s) 2349, 3262, 4041
SspMI CTAG 8 cut(s) 2726, 2777, 2858, 3096, 3303, 3558, 3662, 5003
SstI GAGCTC 1 cut(s) 976
StyD4I CCNGG 8 cut(s) 125, 1219, 1777, 3701, 3922, 4323, 4690, 4913
StyI CCWWGG 5 cut(s) 2254, 2331, 3633, 3949, 4603
TaiI ACGT 6 cut(s) 401, 1589, 1824, 2988, 4158, 4992
TatI WGTACW 6 cut(s) 688, 1034, 1090, 1663, 3002, 4747
TauI GCSGC 2 cut(s) 2091, 3371
TseFI GTSAC 5 cut(s) 1199, 3061, 3163, 3226, 3512
TseI GCWGC 7 cut(s) 168, 171, 1027, 2829, 2953, 3832, 3994
Tsp45I GTSAC 5 cut(s) 1199, 3061, 3163, 3226, 3512
TspGWI ACGGA 5 cut(s) 371, 591, 2958, 4301, 4483
Vha464I CTTAAG 2 cut(s) 1376, 4148
VpaK11BI GGWCC 5 cut(s) 291, 358, 1217, 2242, 3640
XagI CCTNNNNNAGG 1 cut(s) 1509
XbaI TCTAGA 1 cut(s) 5002
XceI RCATGY 5 cut(s) 2080, 2131, 3494, 4369, 4842
XcmI CCANNNNNNNNNTGG 2 cut(s) 2252, 3631
XhoI CTCGAG 2 cut(s) 2912, 3845
XmiI GTMKAC 1 cut(s) 1287
XmnI GAANNNNTTC 8 cut(s) 255, 864, 1343, 1371, 2340, 2636, 4583, 4998
XspI CTAG 8 cut(s) 2726, 2777, 2858, 3096, 3303, 3558, 3662, 5003
Zsp2I ATGCAT 1 cut(s) 1303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.