RLG00000000661

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000129
Physical Location & Seq
Reverse (-)
3273095 .. 3281573
8479 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000661

Sequence Viewer

Length: 3429 bp
ATGTATTGTATGCACAGGTTTGTTGGTGTCTCTTGGGTGGTGGCATCTGGGTGGTGCAATAGTGGTGTTCCTCGTTCTGTGGCGACGGTGGATTCGCTCGTTGTGTGCATGCAATGCGAAGACTGGGTCTGCGCTGAGCCTGTCTTGTTGGGCCAGAAAGGGATTATTGATGTTCTTAAAGTTAGTTTTGATGGATTAGAAGAATCAGAAAAGAAAACGTTTTTGGACATCGCGTGTTTCTTTAAAGGAGAGAACAAGGATCGTGTAATAAGAATACTAAATGGTTGTGACGATGACGGTGTGGACATGGATGTGCAAGTTCTTTTGGAAAAATCTTTGGTAACTCTATTTGGAACAAAATTGTGGATGCATGATTTGATACAAGAAATGGGTTGGGAAGTTGTTCGTCAAAACAACCGTGAAGAGCCTGGAAAACGTAGCAGATTGTGGCTTCCCGATGAAATCATTCCAGTACTTGGTCGGAGTAAGGGATGGAGCATGCTACGGCATTTAGATCTGAGTGGTTCACTATACTTGATGTCCACTCCAGACTTCACTGAGGTTCCAGATCTTGAAATGTTGGTGCTTCAATTTTGTATGAGCTTGGTGGAGGTTCACCCGTCTCTTGGATCTCTGAAGAAACTTGTCTCTTTGAATATGACATATTGCCAGTCCATTGAGAGCATTCCACCTTTCATTGCACTTGAATCCCTTCAAGTATTGAGTCTTTCAACCTGTTCAGGACTGAAGAAGTTTCCAGAAATTGAAGGAAATATGAAAAGCTTGCTGGAGCTTCATTTGGATGGGACCAGCATTGAGGAATTGCCTCCATCAATTGAACGCTTGACTGGTCTAACCATGTTGGATCTGACAGATTGTAAAAACCTTTTGCATCTTCCCAATACCATTGGGTGCTTGACATCTTTGAAAAGTCTCTATCTAACAGATTGCTCCAAACTTGATGAGATACCTGAGAATCTGAATGGTATGAAATGTCTGGAGAAGCTTTTCATTGGTGGAACTTCCATAAGGGAGTTATCTTTCATTTTAGGCATGAAGAATCTACATTATCTGTCCTGCCGAGGATGTAAATGTCTAGTTTCAAAATCATGCAAAGGTCTGACTTCGTTATCAAGTTTGATGGAGCTAGACTTGGGTTATTGCAATCTGATGGATGGAGCAATTCTCAATGATTTTGGCAGCCTAATCTCCTTAAGAAGGTTAGATTTAAGTGGTAATTTCTTTCCGCGATTACCTGAGAGTATCTCTCAATTCCCAAAGCTCGAGGCTCTGGACGTGAGGAATTGTAGACAGCTTCAATTACTGCCCAAGAAGCTTCCGTCAAGTCTTCTAAACGTGTATGCAGAAGGATGTACTGCACTGACGGATTATCCAAATCAAATGAAAGTATTGACTTCACCGGAGTCCGGAGTGACTATTGTCAATTCCCTCAATTCTTCAGCACTTGGAGTCTCACAAAGTTTTGCAATGTTGGATTTTCCAAGCAATGAGGGGGAATCTCTAGAGCTGCGTGTGACATATAACCATACAGAGGGGCAACAAGTTGATCATTTGCCGCTGGCAATTCCAGCGTTTACAAAACAACTGCTTTCGGGGTCAAAATTCAGTACCATACTTCCTCTAGATGAAATTCCAGAGTGGTTCATCACTACATCTACCAGAAATCCCATATCAATCTCAATACCTCCAAATCTGGCCGATGATAACACGTGGAATGGATTTGTCGCATGTGCTATATTCTCAGTCAAGGGGCATGCTGCTCTCTCCCTAATTGAACCGGATCCTGATTTTTCTAGTTACTCATATCAAATCACATTGGAAACTGATGTTATGCGTCTGGAACCATATGTGTTGGGACTGGATTCAGTGGTATTAGGCCGCTTTAGATCAATTTCTCATCTGTTTGCGATTTTCTTTATATCTAGTTTGAAGTTTCCACAGAGGGGATTAAATCAATCAACTGTGGTAAGTGCTATGTTTGAAACCACCAATCCATCCATGGTGGTCCAGAAATGTGGCATTCGTCCAGTCTATGAGCAAGATGCTGGATGGTTCTCCAGAATAATCGGGCGGAATGATTTGATTTGTCTGAATAAAGTTGATGACACCACCTCTATATGGCTTGAACAATACACCGCCTGTGTATTGGAGTCTGGCTGGCTTAGTCAATCTTGGGAATGGGAGGAATTCATTCCACCCTTGGAAGAAGAATCCACACTTGCACTAAATCATTACTGTGCAACTTTTGAATTAAACATCAGAGGAAGCCCACAGTGGTTTCAGTCGTTTGTCTCTAATACGAGGTTAAATTTCACCATAGGGTCAATCAAGCTGCCTCAAAATCTACATAAGAGCAAGAAATGGAAGGGATTTGCAATATACGCATCACTTGTAGAGGAAGTGGGACGGACAATGAAAGAGGACAACTATCGGATATATGTTACACTAACAACTGGGGATATTTCTGGCAAGATGATGTCCCTAGAACTTAAGCATGTTAGGCCATTGTCAGAAGAACATCATCAACTATTGGTTATTTACATACCGCGAGCACAGATTCCCGAAGCGTTGTTCACTCAGACATTAACAACTGCAATGGACTTTTGCATACATATCAGTGATAGCCCTCATGTAAAGGTTCAAAGATGTGGGTTCCGTATTGTGTACCAGGAAGATATACAAGGGTTTGCTGATACAATTATCCGGTGCATGCAAAGGGAAGATTCTCTTGAATATTACAATAAGTTGGTGGTAGAACGATGGATAAAGTTGATACGATTGCAAGGTGCACATATAGAAGGGATGAAAGAATGGGATTTCAGAACACCACGGGAAAAATGCTTTGAATTGCAGTCACGAAAATATAGAAATTGGGAATGGTCTGGCCCTCGTCTTGCTTATTGTGTCTTTGACATGGTTGAAATCTCCAAGTTGAAATGGTTCATGCCTTTCATCAACCAAGGCAACTTTGCAGAAATCCAACTACCTCTGAATGTGTTCGATGATCGTAATTGGTTGGGGTTTGCCGCGTGTTTTCAGTTGTCCGACAACCAATATCCAAACGTTAGTCTTGGCAGCACTGCTCCTGATCCAGAGGATGTTAATGTTATTTGCCGTCTGGACTCTGATGTTGCTTGGCAGTACTTATTTGATGTCGAGATGCATTTAATTTTAAAGAGTCAAGAGCTATTGAAATGCGATACGACTTGGTTCTTTTATATACCGCGAACTGGAGTTCGTAGTAAGGTTTGGAGGCAATGCAAATTGGCAAGGATAACTATGTGGTTCTCTAGGCCATGGTTTGCAGTGCATAGTTGTGCCCTTCGTCTACTATTTAAGGAGGATGTTGAACATCTTGTAGAAACACTGACCCATGCCGAATTACGTACCGTGAAGATCCTCCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1143

Amino Acids

129.98

Weight (kDa)

5.45

Isoelectric Point (pI)

45.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_ROQ1 PF23282 67 - 137 8.8e-14 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 217 - 358 2.7e-13 Leucine-rich repeat region
LRR_13 PF23286 235 - 322 2.6e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 374 - 460 6.2e-07 Leucine-rich repeat region
C-JID PF20160 546 - 689 5.2e-08 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 476
AccI GTMKAC 2 cut(s) 1309, 3349
AccII CGCG 5 cut(s) 233, 1249, 2569, 3050, 3247
AccIII TCCGGA 1 cut(s) 1427
AciI CCGC 8 cut(s) 1247, 1577, 1900, 2092, 2157, 2567, 3048, 3245
AclI AACGTT 2 cut(s) 218, 3084
AclWI GGATC 7 cut(s) 267, 637, 873, 1796, 1809, 3104, 3412
AcoI YGGCCR 1 cut(s) 1716
AcsI RAATTY 4 cut(s) 1622, 1650, 2207, 2329
AcuI CTGAAG 3 cut(s) 656, 767, 1443
AcvI CACGTG 1 cut(s) 1731
AfaI GTAC 6 cut(s) 474, 1375, 1630, 2687, 3164, 3409
AfiI CCNNNNNNNGG 8 cut(s) 476, 626, 1218, 1427, 1552, 1964, 2139, 3251
AflII CTTAAG 2 cut(s) 1213, 2510
AflIII ACRYGT 2 cut(s) 1356, 1728
AjiI CACGTC 1 cut(s) 1297
AjnI CCWGG 2 cut(s) 427, 2688
AjuI GAANNNNNNNTTGG 2 cut(s) 206, 238
Alw21I GWGCWC 2 cut(s) 2575, 2812
Alw26I GTCTC 6 cut(s) 34, 627, 652, 938, 1477, 2317
Alw44I GTGCAC 1 cut(s) 2808
AlwI GGATC 7 cut(s) 267, 637, 873, 1796, 1809, 3104, 3412
Ama87I CYCGRG 1 cut(s) 1283
Aor13HI TCCGGA 1 cut(s) 1427
AoxI GGCC 6 cut(s) 151, 1716, 1897, 2522, 2905, 3314
ApaLI GTGCAC 1 cut(s) 2808
ApeKI GCWGC 5 cut(s) 1200, 1528, 1778, 2353, 3096
ApoI RAATTY 4 cut(s) 1622, 1650, 2207, 2329
ArsI GACNNNNNNTTYG 4 cut(s) 76, 108, 111, 143
Asp700I GAANNNNTTC 7 cut(s) 402, 465, 711, 1007, 2211, 2960, 3017
AspLEI GCGC 1 cut(s) 134
AspS9I GGNCC 4 cut(s) 151, 807, 2026, 2906
AsuHPI GGTGA 3 cut(s) 608, 1410, 2326
AvaI CYCGRG 1 cut(s) 1283
AvaII GGWCC 2 cut(s) 807, 2026
BaeGI GKGCMC 2 cut(s) 2812, 3343
BamHI GGATCC 1 cut(s) 1801
BbrPI CACGTG 1 cut(s) 1731
BbsI GAAGAC 2 cut(s) 126, 1340
Bbv12I GWGCWC 2 cut(s) 2575, 2812
BbvI GCAGC 5 cut(s) 1212, 1515, 1765, 2340, 3108
BceAI ACGGC 2 cut(s) 521, 3120
BciT130I CCWGG 2 cut(s) 429, 2690
BclI TGATCA 1 cut(s) 1567
BcoDI GTCTC 6 cut(s) 34, 627, 652, 938, 1477, 2317
BfaI CTAG 8 cut(s) 1097, 1148, 1523, 1643, 1815, 1944, 2504, 3312
BfrI CTTAAG 2 cut(s) 1213, 2510
BglII AGATCT 2 cut(s) 514, 568
BisI GCNGC 8 cut(s) 1201, 1529, 1577, 1779, 1900, 2354, 3048, 3097
BlpI GCTNAGC 1 cut(s) 135
BlsI GCNGC 8 cut(s) 1202, 1530, 1578, 1780, 1901, 2355, 3049, 3098
BmcAI AGTACT 2 cut(s) 474, 3164
Bme1390I CCNGG 2 cut(s) 429, 2690
Bme18I GGWCC 2 cut(s) 807, 2026
BmeT110I CYCGRG 1 cut(s) 1283
BmgBI CACGTC 1 cut(s) 1297
BmgT120I GGNCC 4 cut(s) 151, 807, 2026, 2906
BmiI GGNNCC 5 cut(s) 564, 808, 1803, 1863, 2675
BmrFI CCNGG 2 cut(s) 429, 2690
BmrI ACTGGG 2 cut(s) 133, 2484
BmsI GCATC 6 cut(s) 53, 357, 901, 2053, 2414, 3171
BmuI ACTGGG 2 cut(s) 133, 2484
BoxI GACNNNNGTC 1 cut(s) 1439
BpiI GAAGAC 2 cut(s) 126, 1340
BplI GAGNNNNNCTC 6 cut(s) 1170, 1202, 1250, 1282, 1252, 1284
BpmI CTGGAG 6 cut(s) 531, 809, 1019, 2062, 3273, 3407
Bpu1102I GCTNAGC 1 cut(s) 135
BsaAI YACGTR 2 cut(s) 1731, 3407
BsaJI CCNNGG 6 cut(s) 1081, 2019, 2220, 2849, 2980, 3317
BsaWI WCCGGW 4 cut(s) 1420, 1427, 1798, 2724
Bsc4I CCNNNNNNNGG 8 cut(s) 476, 626, 1218, 1427, 1552, 1964, 2139, 3251
Bse1I ACTGG 9 cut(s) 128, 470, 670, 853, 1884, 2048, 2479, 3256, 3424
Bse3DI GCAATG 6 cut(s) 119, 696, 1494, 1513, 2622, 3284
BseAI TCCGGA 1 cut(s) 1427
BseBI CCWGG 2 cut(s) 429, 2690
BseDI CCNNGG 6 cut(s) 1081, 2019, 2220, 2849, 2980, 3317
BseLI CCNNNNNNNGG 8 cut(s) 476, 626, 1218, 1427, 1552, 1964, 2139, 3251
BseMI GCAATG 6 cut(s) 119, 696, 1494, 1513, 2622, 3284
BseMII CTCAG 7 cut(s) 126, 509, 549, 963, 1248, 1776, 2612
BseNI ACTGG 9 cut(s) 128, 470, 670, 853, 1884, 2048, 2479, 3256, 3424
BseSI GKGCMC 2 cut(s) 2812, 3343
BseXI GCAGC 5 cut(s) 1212, 1515, 1765, 2340, 3108
BsgI GTGCAG 1 cut(s) 1362
Bsh1236I CGCG 5 cut(s) 233, 1249, 2569, 3050, 3247
BshFI GGCC 6 cut(s) 153, 1718, 1899, 2524, 2907, 3316
BsiHKAI GWGCWC 2 cut(s) 2575, 2812
BsiHKCI CYCGRG 1 cut(s) 1283
BsiSI CCGG 4 cut(s) 1421, 1428, 1799, 2725
BslFI GGGAC 4 cut(s) 820, 1890, 2439, 2485
BslI CCNNNNNNNGG 8 cut(s) 476, 626, 1218, 1427, 1552, 1964, 2139, 3251
BsmAI GTCTC 6 cut(s) 34, 627, 652, 938, 1477, 2317
BsmBI CGTCTC 1 cut(s) 627
BsmFI GGGAC 4 cut(s) 820, 1890, 2439, 2485
BsmI GAATGC 2 cut(s) 684, 2040
BsnI GGCC 6 cut(s) 153, 1718, 1899, 2524, 2907, 3316
BsoBI CYCGRG 1 cut(s) 1283
Bsp1286I GDGCHC 3 cut(s) 2575, 2812, 3343
Bsp13I TCCGGA 1 cut(s) 1427
Bsp1720I GCTNAGC 1 cut(s) 135
Bsp19I CCATGG 2 cut(s) 2019, 3317
BspACI CCGC 8 cut(s) 1247, 1577, 1900, 2092, 2157, 2567, 3048, 3245
BspANI GGCC 6 cut(s) 153, 1718, 1899, 2524, 2907, 3316
BspCNI CTCAG 7 cut(s) 127, 510, 550, 964, 1249, 1775, 2611
BspEI TCCGGA 1 cut(s) 1427
BspFNI CGCG 5 cut(s) 233, 1249, 2569, 3050, 3247
BspLI GGNNCC 5 cut(s) 564, 808, 1803, 1863, 2675
BspPI GGATC 7 cut(s) 267, 637, 873, 1796, 1809, 3104, 3412
BspQI GCTCTTC 1 cut(s) 417
BspTI CTTAAG 2 cut(s) 1213, 2510
BsrDI GCAATG 6 cut(s) 119, 696, 1494, 1513, 2622, 3284
BsrI ACTGG 9 cut(s) 128, 470, 670, 853, 1884, 2048, 2479, 3256, 3424
BssECI CCNNGG 6 cut(s) 1081, 2019, 2220, 2849, 2980, 3317
BssT1I CCWWGG 4 cut(s) 2019, 2220, 2980, 3317
Bst2UI CCWGG 2 cut(s) 429, 2690
Bst4CI ACNGT 7 cut(s) 88, 299, 419, 1984, 2258, 2295, 3412
Bst6I CTCTTC 1 cut(s) 417
BstAFI CTTAAG 2 cut(s) 1213, 2510
BstAPI GCANNNNNTGC 1 cut(s) 114
BstBAI YACGTR 2 cut(s) 1731, 3407
BstC8I GCNNGC 8 cut(s) 110, 500, 785, 1581, 1776, 2180, 2571, 2732
BstDEI CTNAG 8 cut(s) 135, 518, 558, 972, 1257, 1762, 2183, 2598
BstDSI CCRYGG 3 cut(s) 2019, 2849, 3317
BstENI CCTNNNNNAGG 1 cut(s) 1216
BstFNI CGCG 5 cut(s) 233, 1249, 2569, 3050, 3247
BstHHI GCGC 1 cut(s) 134
BstMAI GTCTC 6 cut(s) 34, 627, 652, 938, 1477, 2317
BstMWI GCNNNNNNNGC 5 cut(s) 114, 1333, 1589, 2402, 2521
BstNI CCWGG 2 cut(s) 429, 2690
BstNSI RCATGY 6 cut(s) 112, 502, 1752, 1778, 2519, 2734
BstPAI GACNNNNGTC 1 cut(s) 1439
BstSCI CCNGG 2 cut(s) 427, 2688
BstSLI GKGCMC 2 cut(s) 2812, 3343
BstSNI TACGTA 1 cut(s) 3407
BstUI CGCG 5 cut(s) 233, 1249, 2569, 3050, 3247
BstV1I GCAGC 5 cut(s) 1212, 1515, 1765, 2340, 3108
BstV2I GAAGAC 2 cut(s) 126, 1340
BstX2I RGATCY 6 cut(s) 514, 568, 629, 865, 1801, 3417
BstXI CCANNNNNNTGG 1 cut(s) 2036
BstYI RGATCY 6 cut(s) 514, 568, 629, 865, 1801, 3417
BsuRI GGCC 6 cut(s) 153, 1718, 1899, 2524, 2907, 3316
BtgI CCRYGG 3 cut(s) 2019, 2849, 3317
BtgZI GCGATG 1 cut(s) 214
BtrI CACGTC 1 cut(s) 1297
BtsI GCAGTG 2 cut(s) 3099, 3333
BtsIMutI CAGTG 8 cut(s) 555, 1379, 1893, 2300, 2644, 3099, 3333, 3386
Cac8I GCNNGC 8 cut(s) 110, 500, 785, 1581, 1776, 2180, 2571, 2732
CfoI GCGC 1 cut(s) 134
Cfr13I GGNCC 4 cut(s) 151, 807, 2026, 2906
CseI GACGC 1 cut(s) 1844
Csp6I GTAC 6 cut(s) 473, 1374, 1629, 2686, 3163, 3408
CviQI GTAC 6 cut(s) 473, 1374, 1629, 2686, 3163, 3408
DdeI CTNAG 8 cut(s) 135, 518, 558, 972, 1257, 1762, 2183, 2598
DraI TTTAAA 2 cut(s) 244, 3195
EaeI YGGCCR 1 cut(s) 1716
Eam1104I CTCTTC 1 cut(s) 417
EarI CTCTTC 1 cut(s) 417
EciI GGCGGA 1 cut(s) 2107
Eco105I TACGTA 1 cut(s) 3407
Eco130I CCWWGG 4 cut(s) 2019, 2220, 2980, 3317
Eco47I GGWCC 2 cut(s) 807, 2026
Eco57I CTGAAG 3 cut(s) 656, 767, 1443
Eco72I CACGTG 1 cut(s) 1731
Eco88I CYCGRG 1 cut(s) 1283
EcoNI CCTNNNNNAGG 1 cut(s) 1216
EcoRI GAATTC 1 cut(s) 2207
EcoRII CCWGG 2 cut(s) 427, 2688
EcoT14I CCWWGG 4 cut(s) 2019, 2220, 2980, 3317
EcoT22I ATGCAT 2 cut(s) 372, 3186
ErhI CCWWGG 4 cut(s) 2019, 2220, 2980, 3317
Esp3I CGTCTC 1 cut(s) 627
FalI AAGNNNNNCTT 2 cut(s) 2733, 2765
FaqI GGGAC 4 cut(s) 820, 1890, 2439, 2485
FauNDI CATATG 1 cut(s) 1867
FbaI TGATCA 1 cut(s) 1567
FblI GTMKAC 2 cut(s) 1309, 3349
Fnu4HI GCNGC 8 cut(s) 1201, 1529, 1577, 1779, 1900, 2354, 3048, 3097
Fsp4HI GCNGC 8 cut(s) 1201, 1529, 1577, 1779, 1900, 2354, 3048, 3097
FspBI CTAG 8 cut(s) 1097, 1148, 1523, 1643, 1815, 1944, 2504, 3312
GlaI GCGC 1 cut(s) 133
GluI GCNGC 8 cut(s) 1201, 1529, 1577, 1779, 1900, 2354, 3048, 3097
GsuI CTGGAG 6 cut(s) 531, 809, 1019, 2062, 3273, 3407
HaeIII GGCC 6 cut(s) 153, 1718, 1899, 2524, 2907, 3316
HapII CCGG 4 cut(s) 1421, 1428, 1799, 2725
HgaI GACGC 1 cut(s) 1844
HhaI GCGC 1 cut(s) 134
Hin6I GCGC 1 cut(s) 132
HinP1I GCGC 1 cut(s) 132
HindIII AAGCTT 3 cut(s) 781, 1004, 1334
HpaII CCGG 4 cut(s) 1421, 1428, 1799, 2725
HphI GGTGA 3 cut(s) 608, 1410, 2326
Hpy99I CGWCG 1 cut(s) 88
HpyAV CCTTC 7 cut(s) 722, 761, 1212, 1361, 2380, 2813, 3353
HpyCH4III ACNGT 7 cut(s) 88, 299, 419, 1984, 2258, 2295, 3412
HpyCH4IV ACGT 7 cut(s) 218, 436, 1296, 1356, 1730, 3084, 3406
HpyF10VI GCNNNNNNNGC 5 cut(s) 114, 1333, 1589, 2402, 2521
HpyF3I CTNAG 8 cut(s) 135, 518, 558, 972, 1257, 1762, 2183, 2598
HpySE526I ACGT 7 cut(s) 218, 436, 1296, 1356, 1730, 3084, 3406
HspAI GCGC 1 cut(s) 132
Kpn2I TCCGGA 1 cut(s) 1427
Ksp22I TGATCA 1 cut(s) 1567
LguI GCTCTTC 1 cut(s) 417
LmnI GCTCC 6 cut(s) 495, 790, 956, 1144, 1178, 3109
Lsp1109I GCAGC 5 cut(s) 1212, 1515, 1765, 2340, 3108
LweI GCATC 6 cut(s) 53, 357, 901, 2053, 2414, 3171
MaeI CTAG 8 cut(s) 1097, 1148, 1523, 1643, 1815, 1944, 2504, 3312
MaeII ACGT 7 cut(s) 218, 436, 1296, 1356, 1730, 3084, 3406
MaeIII GTNAC 7 cut(s) 287, 340, 1432, 1534, 1817, 2461, 2874
MfeI CAATTG 1 cut(s) 834
MflI RGATCY 6 cut(s) 514, 568, 629, 865, 1801, 3417
MhlI GDGCHC 3 cut(s) 2575, 2812, 3343
MlyI GAGTC 6 cut(s) 733, 1433, 1479, 2180, 3137, 3208
MmeI TCCRAC 5 cut(s) 461, 843, 1473, 3025, 3090
Mph1103I ATGCAT 2 cut(s) 372, 3186
MroI TCCGGA 1 cut(s) 1427
MroXI GAANNNNTTC 7 cut(s) 402, 465, 711, 1007, 2211, 2960, 3017
MslI CAYNNNNRTG 6 cut(s) 49, 311, 801, 2256, 2637, 3336
MspA1I CMGCKG 1 cut(s) 1579
MspCI CTTAAG 2 cut(s) 1213, 2510
MspI CCGG 4 cut(s) 1421, 1428, 1799, 2725
MspR9I CCNGG 2 cut(s) 429, 2690
MunI CAATTG 1 cut(s) 834
Mva1269I GAATGC 2 cut(s) 684, 2040
MvaI CCWGG 2 cut(s) 429, 2690
MvnI CGCG 5 cut(s) 233, 1249, 2569, 3050, 3247
MwoI GCNNNNNNNGC 5 cut(s) 114, 1333, 1589, 2402, 2521
NcoI CCATGG 2 cut(s) 2019, 3317
NdeI CATATG 1 cut(s) 1867
NlaIV GGNNCC 5 cut(s) 564, 808, 1803, 1863, 2675
NmeAIII GCCGAG 1 cut(s) 1106
NmuCI GTSAC 4 cut(s) 287, 1432, 1534, 2874
NsiI ATGCAT 2 cut(s) 372, 3186
NspI RCATGY 6 cut(s) 112, 502, 1752, 1778, 2519, 2734
PaeI GCATGC 4 cut(s) 112, 502, 1778, 2734
PaeR7I CTCGAG 1 cut(s) 1283
PciSI GCTCTTC 1 cut(s) 417
PctI GAATGC 2 cut(s) 684, 2040
PdmI GAANNNNTTC 7 cut(s) 402, 465, 711, 1007, 2211, 2960, 3017
PflFI GACNNNGTC 1 cut(s) 125
PflMI CCANNNNNTGG 1 cut(s) 476
PkrI GCNGC 8 cut(s) 1202, 1530, 1578, 1780, 1901, 2355, 3049, 3098
PleI GAGTC 6 cut(s) 732, 1432, 1478, 2179, 3137, 3207
PmaCI CACGTG 1 cut(s) 1731
PmlI CACGTG 1 cut(s) 1731
PpsI GAGTC 6 cut(s) 732, 1432, 1478, 2179, 3137, 3207
Ppu21I YACGTR 2 cut(s) 1731, 3407
PshAI GACNNNNGTC 1 cut(s) 1439
Psp1406I AACGTT 2 cut(s) 218, 3084
Psp6I CCWGG 2 cut(s) 427, 2688
PspCI CACGTG 1 cut(s) 1731
PspGI CCWGG 2 cut(s) 427, 2688
PspN4I GGNNCC 5 cut(s) 564, 808, 1803, 1863, 2675
PspPI GGNCC 4 cut(s) 151, 807, 2026, 2906
PspXI VCTCGAGB 1 cut(s) 1283
PsuI RGATCY 6 cut(s) 514, 568, 629, 865, 1801, 3417
PsyI GACNNNGTC 1 cut(s) 125
RsaI GTAC 6 cut(s) 474, 1375, 1630, 2687, 3164, 3409
RsaNI GTAC 6 cut(s) 473, 1374, 1629, 2686, 3163, 3408
RseI CAYNNNNRTG 6 cut(s) 49, 311, 801, 2256, 2637, 3336
SapI GCTCTTC 1 cut(s) 417
SatI GCNGC 8 cut(s) 1201, 1529, 1577, 1779, 1900, 2354, 3048, 3097
Sau96I GGNCC 4 cut(s) 151, 807, 2026, 2906
ScaI AGTACT 2 cut(s) 474, 3164
SchI GAGTC 6 cut(s) 733, 1433, 1479, 2180, 3137, 3208
ScrFI CCNGG 2 cut(s) 429, 2690
SduI GDGCHC 3 cut(s) 2575, 2812, 3343
SfaNI GCATC 6 cut(s) 53, 357, 901, 2053, 2414, 3171
Sfr274I CTCGAG 1 cut(s) 1283
SinI GGWCC 2 cut(s) 807, 2026
SlaI CTCGAG 1 cut(s) 1283
SmiMI CAYNNNNRTG 6 cut(s) 49, 311, 801, 2256, 2637, 3336
SmlI CTYRAG 3 cut(s) 1213, 1283, 2510
SmoI CTYRAG 3 cut(s) 1213, 1283, 2510
SnaBI TACGTA 1 cut(s) 3407
SphI GCATGC 4 cut(s) 112, 502, 1778, 2734
SsiI CCGC 8 cut(s) 1247, 1577, 1900, 2092, 2157, 2567, 3048, 3245
SspI AATATT 1 cut(s) 2756
SspMI CTAG 8 cut(s) 1097, 1148, 1523, 1643, 1815, 1944, 2504, 3312
StyD4I CCNGG 2 cut(s) 427, 2688
StyI CCWWGG 4 cut(s) 2019, 2220, 2980, 3317
TaaI ACNGT 7 cut(s) 88, 299, 419, 1984, 2258, 2295, 3412
TaiI ACGT 7 cut(s) 221, 439, 1299, 1359, 1733, 3087, 3409
TaqI TCGA 3 cut(s) 1284, 3021, 3177
TatI WGTACW 3 cut(s) 472, 1373, 3162
TauI GCSGC 3 cut(s) 1579, 1902, 3050
TscAI CASTG 8 cut(s) 562, 1386, 1893, 2300, 2644, 3106, 3333, 3393
TseFI GTSAC 4 cut(s) 287, 1432, 1534, 2874
TseI GCWGC 5 cut(s) 1200, 1528, 1778, 2353, 3096
Tsp45I GTSAC 4 cut(s) 287, 1432, 1534, 2874
TspGWI ACGGA 4 cut(s) 1329, 1400, 2443, 2666
TspRI CASTG 8 cut(s) 562, 1386, 1893, 2300, 2644, 3106, 3333, 3393
Tth111I GACNNNGTC 1 cut(s) 125
Van91I CCANNNNNTGG 1 cut(s) 476
Vha464I CTTAAG 2 cut(s) 1213, 2510
VneI GTGCAC 1 cut(s) 2808
VpaK11BI GGWCC 2 cut(s) 807, 2026
XagI CCTNNNNNAGG 1 cut(s) 1216
XapI RAATTY 4 cut(s) 1622, 1650, 2207, 2329
XbaI TCTAGA 2 cut(s) 1522, 1642
XceI RCATGY 6 cut(s) 112, 502, 1752, 1778, 2519, 2734
XcmI CCANNNNNNNNNTGG 1 cut(s) 2017
XhoI CTCGAG 1 cut(s) 1283
XmiI GTMKAC 2 cut(s) 1309, 3349
XmnI GAANNNNTTC 7 cut(s) 402, 465, 711, 1007, 2211, 2960, 3017
XspI CTAG 8 cut(s) 1097, 1148, 1523, 1643, 1815, 1944, 2504, 3312
ZrmI AGTACT 2 cut(s) 474, 3164
Zsp2I ATGCAT 2 cut(s) 372, 3186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.