Rroxscaffold_3G00219810

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000129
Physical Location & Seq
Reverse (-)
1965124 .. 1971242
6119 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00219810.1

Sequence Viewer

Length: 3513 bp
ATGTTTTTGGACATCGCATGTTTCTTTAAAGGAGCGTATAGAGATCATGTTAAAAGAATACTACTAGGTAGTGACCACAGTCCGGACATTGATATAGAAGTTCTCATAGAGAAATCTTTGGTAACTTTGTTTGGAAGAAGATTAGGGATGCATGACTTGGTACAAGAATTGGGTTGGGAAATTGTTCGTCAAGAATGTCGTGAAGAGCCTGGAAAACGTAGTAGGTTGTGGCTTTCTGATGAAATCATTCATGTACTTGGTCGGAGTAAGGCGACGAGTGCAGTTCAAAGCATATCCCTCCAATGTCGAAGAGAAGATGATGTTGTCCACTCAAGTGATGATGCCTTTTCAAATATGGACAGATTGAGATTGCTCAAGATTTGTAATTTGAAGTTCTCTGAAAACATCAGATATCTTTCTAATGAGTTACAGTATCTTGAATGGCATAAATGTCCTTTGGATTTTTTCCCGTCAGACTTTCAACCGGATAAACTTACCGTGGTTCTTATGTTTCGTAGCCGCATCAAACAACTATGGAGGGGAAAAAAAGGATGGAGCATGCTACGGGTTATTAATCTGAGTGGTTCAAAATACTTGATGTCGACTCCAGACTTCACTGAGGTTCCAGATCTTGAAGAGTTGGTGCTTCAAAATTGCACAAGCTTGGTGGAGGTTCACCCATCTCTCGGATTTCTGAAGAAACTTGGCTATTTGGATATGAAAAATTGCGAGTCTATTGAGAGCATTCCACCTTTCACTACCTTGGAATCCCTTCAAATATTGAGGCTTTCACTCTGTTCAGGACTGAAGAAGGTTCCAGAAATTGAAGGAAATATGAAAAGCTTGCTGGAGCTTTATTTGAATGGGACCAGCATTGAGGAATTACCTCCATCAATTGACCGTTTGACTGGTCTTACCACGTTGGATCTGACAGATTGTAAAAACCTTTTGCATCTTCCCAATACCACTGGGTGCTTGACATCTTTAAAAAGTCTCCGTCTAGCAGGTTGCTCCAAAATTGATGAGGTTCCTAAGAATCTGAATGGTATGAAACGTCTGGAGATGCTTACAATTGGTGGAACTTCCATAAGGGAGTTATCATTCATAGTAGGCATGAAGAATCTACAGTTACTATGCTGCGAAGGATGTAAATGCCTAGTTTCAGGATCATGTAAAGTCCTGGCTTCCTTATCGAGTTTGATAGAGCTACACTTGAATGATTGCAATCTGATGGATGGAGAAATTCTCAATGATCTTAGCAGCCTAATCTCCTTAAGAATGTTAGATTTAAGTGGTAATTGCTTTGTACGATTACCTGAAAGTATCTCTCAACTCTCAAAGCTCAATACTCTGCGCTTGACGAATTGTAGACAACTTCAATTGCTGCCCAAGAAGCTTCCGTTAAGTCTTCGAAACGTGTATGCACAAGATTGTACTTCACTGACGGATTACCCAAATCAAATCAAAGTATTGAATTCATCGGAGTCGGGAGTGACTATTGTCAACTCCCTCAATTCTTCAGCACTTGAAGTCTCAGAATCCAGAGCGGTCATTTATCCAAGAAATGAGGGGGAATCTACAGAGCTGCTTTTGACACATGAGAATACAGAGGGGAAACAAGTTGATCATTATTTGCCACTGAGAAGTGTAGCGCATCAAAGAGAAGAAGTGTCTTCCTGGTCAAATTTCGCTATCGTAATTCCTACAGATGAAATTCCAGAGTGTTTCAGCACTATATCTACCAGAAATCCCATATCAATCCCGATGCCTCAAAATCTGACCGATGATAACAAGTGGAAGGGACTTGCCGCGTGTGCTATATTCTCAGTCAAGGGGCATACTGCTCTCTCTGTTATTGAACCGGATCCTGAATTTTCTAATTACTCGTATCAAATGACATTGGAAACCGATGCTGGGCGTCTGAAACCATATGTGTTGGATAGGAAAGACCTTCTTCCCCCTCTTGGATCAAGTTCTCATCTGCTTATGATTTTCTATATATCTAGTTCAAAGAAGTACTATCCACAATGGGAATTAGACATTTCAACTGCGGTGAGTGCTACGTTTGAAACCACCAATTCATCCATGGTGGTCCAGAAATGTGGAATTGGTCTAGTCTATGAACAAGATGCTGGAATGTATTCCAGGATAGTCGATGGGGATCAACCGATTTGTCTGAATGAAGTTGAGGACGCCGCCTCTATACGGGAGTCTAGCTGGCTTAGTCAATCATTAAAATGGGAGAAATTCATTCCACCCTTGGAAGAACAATCCAGACTTGTGCTGAGAAAGAACCTTGAGTCTGTTCTTCCAAGATACCTTGAGGTCGATCCAACTGCGCTAAACCGTTCTTCTACAACGTACAAATTTAACCTCAGAGGAAGCCCAGGGTGGTTTCATAAGTCATTTTCCCCTGGCACGAGGTCAGATTTTATCGTAGTGTCAATCAAGCTGCCCCAAAATCTACACACGAGCAAGAAGTGGATGGGATTTGCAATATACGCATCACTTGCAGAGGAAGTGGGGCAGCAGACAATGAAAGAGGACAACTATTGGGTATCTGTTACACTATCAACAGGGGATAAATTTGGTACGGGTGTGTCTCGAGTACTTAAGCACATCAGGCCATTGTCAGAAGAACATCATCAACTATTGGTTATTTACATACCGCGGGCAGAGATTCCCGAAGGGTTGTTCACTCAGACATTAACAACTGCAATGGACTTTTGTATCATGATCAGTGATAGCCCTCATGTAAAGGTTCAAAGATGTGGGTCACGTATTGTGTACCAGGAAGATATACAAGGTTTTTCTGATACAATTATCCGGTGCATGCAGAGGGATGATTCTCTTAAATCTCACGATAAGTTGGTGGTAGAAGCATGGTTACAGTTGATACAATTGCAAGGTGCCCATATAGAGAGAATGAAAGAACGGGATTCTAGAACCCCACTGGAAAAATACTTTGAATTGCACTCACGAAAATATCGAAATTGGGACTGGTCTGACCCTCGTCCAGTTTCTTGTCACATCGAGGGAGCTGAAATTTTCAAGTCAAAATGGTTCATGCCTTTCATCAACCAAGGCAACTCTGCAGAAATCCAATTGCCTCTGAATGTGTTCGATGATAATAATTGGTTGGGGTTTGCCGTATGTTCTGGGTTGTCCCATGACCAATATCCTAACATTAGTCTTGGCAGTACTGCCCCTGATTTAGAGAATGTTATGCATAGTCTTCGTTTGCACTCTGATGCTGCTTGGCACGGCTTATTTGATATCACCATGAAGGCATATTTTAGGATACTCGAGCCATTGGAATACGATGCAACTTTGTTCTTTTATATACCGCGCACTAATTGTCTCAGTAAGATGTGGAGGCAATGCAAATTGGCCAGGGTAACTATATGGTGCTCTAGCCCATGCGTAGTTGTGCATAGTTGTGTCCTTCGTCTAGTATTTAACGAGGACGTTGAACATCTCGTAGAAACATTGACCCTCGCCGAATTACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1170

Amino Acids

132.9

Weight (kDa)

5.94

Isoelectric Point (pI)

48.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_ROQ1 PF23282 1 - 64 1e-13 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 224 - 336 7.7e-09 Leucine-rich repeat region
LRR_13 PF23286 255 - 343 5.6e-12 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 285 - 405 5.2e-10 Leucine-rich repeat region
LRR_14 PF23598 394 - 493 3e-07 Leucine-rich repeat region
C-JID PF20160 568 - 711 5.6e-07 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 995
AccB1I GGYRCC 1 cut(s) 2909
AccBSI CCGCTC 1 cut(s) 1547
AccI GTMKAC 2 cut(s) 602, 1369
AccII CGCG 3 cut(s) 1811, 2671, 3349
AccIII TCCGGA 1 cut(s) 82
AciI CCGC 8 cut(s) 520, 1547, 1809, 2051, 2196, 2669, 2671, 3347
AclWI GGATC 7 cut(s) 933, 1174, 1859, 1872, 1975, 2169, 2324
AcoI YGGCCR 1 cut(s) 3390
AcsI RAATTY 9 cut(s) 1242, 1474, 1684, 1713, 1871, 2246, 2366, 2585, 3045
AcuI CTGAAG 3 cut(s) 716, 827, 1503
AcyI GRCGYC 2 cut(s) 1918, 2193
AdeI CACNNNGTG 1 cut(s) 972
AfiI CCNNNNNNNGG 5 cut(s) 82, 686, 1914, 1964, 2205
AflII CTTAAG 2 cut(s) 1273, 2612
AflIII ACRYGT 1 cut(s) 1416
AjnI CCWGG 8 cut(s) 208, 1179, 1676, 2144, 2386, 2413, 2790, 3392
AleI CACNNNNGTG 1 cut(s) 333
Alw21I GWGCWC 1 cut(s) 3413
Alw26I GTCTC 4 cut(s) 998, 1537, 2607, 3365
AlwI GGATC 7 cut(s) 933, 1174, 1859, 1872, 1975, 2169, 2324
Ama87I CYCGRG 2 cut(s) 2604, 3305
Aor13HI TCCGGA 1 cut(s) 82
AoxI GGCC 2 cut(s) 2624, 3390
ApeKI GCWGC 7 cut(s) 1137, 1260, 1384, 1585, 2452, 2527, 3254
ApoI RAATTY 9 cut(s) 1242, 1474, 1684, 1713, 1871, 2246, 2366, 2585, 3045
AseI ATTAAT 1 cut(s) 573
Asp700I GAANNNNTTC 5 cut(s) 183, 246, 771, 2140, 3119
AspLEI GCGC 4 cut(s) 1356, 1654, 2341, 3351
AspS9I GGNCC 2 cut(s) 867, 2092
AsuHPI GGTGA 3 cut(s) 668, 2065, 3271
AsuII TTCGAA 1 cut(s) 1411
AvaI CYCGRG 2 cut(s) 2604, 3305
AvaII GGWCC 2 cut(s) 867, 2092
BaeGI GKGCMC 1 cut(s) 2914
BaeI ACNNNNGTAYC 4 cut(s) 2583, 2616, 2713, 2746
BalI TGGCCA 1 cut(s) 3392
BamHI GGATCC 1 cut(s) 1864
BanI GGYRCC 1 cut(s) 2909
BarI GAAGNNNNNNTAC 2 cut(s) 2871, 2903
BauI CACGAG 2 cut(s) 2419, 2470
BbsI GAAGAC 3 cut(s) 1400, 1665, 3227
Bbv12I GWGCWC 1 cut(s) 3413
BbvI GCAGC 7 cut(s) 1124, 1272, 1371, 1572, 2439, 2539, 3241
BccI CCATC 7 cut(s) 546, 688, 898, 1225, 1229, 2150, 2479
BceAI ACGGC 2 cut(s) 3134, 3280
BcgI CGANNNNNNTGC 4 cut(s) 2318, 2352, 3218, 3252
BciT130I CCWGG 8 cut(s) 210, 1181, 1678, 2146, 2388, 2415, 2792, 3394
BciVI GTATCC 1 cut(s) 3294
BclI TGATCA 2 cut(s) 1624, 2736
BcoDI GTCTC 4 cut(s) 998, 1537, 2607, 3365
BfaI CTAG 9 cut(s) 65, 1001, 1157, 2004, 2114, 2214, 2943, 3414, 3452
BfmI CTRYAG 4 cut(s) 1124, 1578, 1704, 3093
BfrI CTTAAG 2 cut(s) 1273, 2612
BfuAI ACCTGC 1 cut(s) 995
BfuI GTATCC 1 cut(s) 3294
BglII AGATCT 1 cut(s) 628
BmcAI AGTACT 3 cut(s) 2018, 2610, 3202
Bme1390I CCNGG 8 cut(s) 210, 1181, 1678, 2146, 2388, 2415, 2792, 3394
Bme18I GGWCC 2 cut(s) 867, 2092
BmeT110I CYCGRG 2 cut(s) 2604, 3305
BmgT120I GGNCC 2 cut(s) 867, 2092
BmiI GGNNCC 6 cut(s) 624, 816, 868, 1029, 1866, 2911
BmrFI CCNGG 8 cut(s) 210, 1181, 1678, 2146, 2388, 2415, 2792, 3394
BmrI ACTGGG 1 cut(s) 978
BmuI ACTGGG 1 cut(s) 978
BoxI GACNNNNGTC 3 cut(s) 78, 1499, 3012
BpiI GAAGAC 3 cut(s) 1400, 1665, 3227
BplI GAGNNNNNCTC 2 cut(s) 1230, 1262
BpmI CTGGAG 3 cut(s) 591, 869, 1079
Bpu14I TTCGAA 1 cut(s) 1411
BpuEI CTTGAG 4 cut(s) 316, 359, 2318, 2342
BsaAI YACGTR 1 cut(s) 2780
BsaBI GATNNNNATC 2 cut(s) 1224, 2160
BsaHI GRCGYC 2 cut(s) 1918, 2193
BsaWI WCCGGW 4 cut(s) 82, 484, 1861, 2826
Bsc4I CCNNNNNNNGG 5 cut(s) 82, 686, 1914, 1964, 2205
Bse1I ACTGG 5 cut(s) 913, 973, 2958, 3005, 3017
Bse3DI GCAATG 2 cut(s) 2724, 3386
Bse8I GATNNNNATC 2 cut(s) 1224, 2160
BseAI TCCGGA 1 cut(s) 82
BseBI CCWGG 8 cut(s) 210, 1181, 1678, 2146, 2388, 2415, 2792, 3394
BseGI GGATG 7 cut(s) 153, 557, 1151, 1240, 2081, 2490, 2848
BseJI GATNNNNATC 2 cut(s) 1224, 2160
BseLI CCNNNNNNNGG 5 cut(s) 82, 686, 1914, 1964, 2205
BseMI GCAATG 2 cut(s) 2724, 3386
BseMII CTCAG 9 cut(s) 569, 609, 1548, 1631, 1839, 2276, 2389, 2714, 3376
BseNI ACTGG 5 cut(s) 913, 973, 2958, 3005, 3017
BseSI GKGCMC 1 cut(s) 2914
BseXI GCAGC 7 cut(s) 1124, 1272, 1371, 1572, 2439, 2539, 3241
BseYI CCCAGC 1 cut(s) 1913
BsgI GTGCAG 1 cut(s) 300
Bsh1236I CGCG 3 cut(s) 1811, 2671, 3349
BshFI GGCC 2 cut(s) 2626, 3392
BshNI GGYRCC 1 cut(s) 2909
BsiHKAI GWGCWC 1 cut(s) 3413
BsiHKCI CYCGRG 2 cut(s) 2604, 3305
BsiSI CCGG 4 cut(s) 83, 485, 1862, 2827
BslFI GGGAC 4 cut(s) 880, 1815, 3011, 3151
BslI CCNNNNNNNGG 5 cut(s) 82, 686, 1914, 1964, 2205
BsmAI GTCTC 4 cut(s) 998, 1537, 2607, 3365
BsmFI GGGAC 4 cut(s) 880, 1815, 3011, 3151
BsmI GAATGC 1 cut(s) 744
BsnI GGCC 2 cut(s) 2626, 3392
BsoBI CYCGRG 2 cut(s) 2604, 3305
Bsp119I TTCGAA 1 cut(s) 1411
Bsp1286I GDGCHC 2 cut(s) 2914, 3413
Bsp13I TCCGGA 1 cut(s) 82
Bsp19I CCATGG 1 cut(s) 2085
BspACI CCGC 8 cut(s) 520, 1547, 1809, 2051, 2196, 2669, 2671, 3347
BspANI GGCC 2 cut(s) 2626, 3392
BspCNI CTCAG 9 cut(s) 570, 610, 1547, 1632, 1838, 2277, 2388, 2713, 3375
BspEI TCCGGA 1 cut(s) 82
BspFNI CGCG 3 cut(s) 1811, 2671, 3349
BspHI TCATGA 1 cut(s) 2733
BspLI GGNNCC 6 cut(s) 624, 816, 868, 1029, 1866, 2911
BspMAI CTGCAG 1 cut(s) 3097
BspMI ACCTGC 1 cut(s) 995
BspPI GGATC 7 cut(s) 933, 1174, 1859, 1872, 1975, 2169, 2324
BspQI GCTCTTC 1 cut(s) 198
BspT104I TTCGAA 1 cut(s) 1411
BspT107I GGYRCC 1 cut(s) 2909
BspTI CTTAAG 2 cut(s) 1273, 2612
BsrBI CCGCTC 1 cut(s) 1547
BsrDI GCAATG 2 cut(s) 2724, 3386
BsrI ACTGG 5 cut(s) 913, 973, 2958, 3005, 3017
BssNI GRCGYC 2 cut(s) 1918, 2193
BssSI CACGAG 2 cut(s) 2419, 2470
BssT1I CCWWGG 4 cut(s) 762, 2085, 2259, 3082
Bst2BI CACGAG 2 cut(s) 2419, 2470
Bst2UI CCWGG 8 cut(s) 210, 1181, 1678, 2146, 2388, 2415, 2792, 3394
Bst4CI ACNGT 7 cut(s) 80, 432, 499, 902, 1128, 2348, 2892
Bst6I CTCTTC 3 cut(s) 198, 304, 630
BstACI GRCGYC 2 cut(s) 1918, 2193
BstAFI CTTAAG 2 cut(s) 1273, 2612
BstAPI GCANNNNNTGC 1 cut(s) 2510
BstBAI YACGTR 1 cut(s) 2780
BstBI TTCGAA 1 cut(s) 1411
BstC8I GCNNGC 5 cut(s) 560, 845, 2219, 2673, 2834
BstDSI CCRYGG 3 cut(s) 498, 2085, 2669
BstF5I GGATG 7 cut(s) 153, 557, 1151, 1240, 2081, 2490, 2848
BstFNI CGCG 3 cut(s) 1811, 2671, 3349
BstHHI GCGC 4 cut(s) 1356, 1654, 2341, 3351
BstMAI GTCTC 4 cut(s) 998, 1537, 2607, 3365
BstMWI GCNNNNNNNGC 7 cut(s) 278, 1393, 1814, 2057, 2501, 2510, 2623
BstNI CCWGG 8 cut(s) 210, 1181, 1678, 2146, 2388, 2415, 2792, 3394
BstNSI RCATGY 3 cut(s) 21, 562, 2836
BstPAI GACNNNNGTC 3 cut(s) 78, 1499, 3012
BstSCI CCNGG 8 cut(s) 208, 1179, 1676, 2144, 2386, 2413, 2790, 3392
BstSFI CTRYAG 4 cut(s) 1124, 1578, 1704, 3093
BstSLI GKGCMC 1 cut(s) 2914
BstUI CGCG 3 cut(s) 1811, 2671, 3349
BstV1I GCAGC 7 cut(s) 1124, 1272, 1371, 1572, 2439, 2539, 3241
BstV2I GAAGAC 3 cut(s) 1400, 1665, 3227
BstX2I RGATCY 3 cut(s) 628, 925, 1864
BstXI CCANNNNNNTGG 1 cut(s) 2102
BstYI RGATCY 3 cut(s) 628, 925, 1864
BsuI GTATCC 1 cut(s) 3294
BsuRI GGCC 2 cut(s) 2626, 3392
BtgI CCRYGG 3 cut(s) 498, 2085, 2669
BtsCI GGATG 7 cut(s) 153, 557, 1151, 1240, 2081, 2490, 2848
BtsIMutI CAGTG 6 cut(s) 615, 966, 1439, 1637, 2746, 2951
BveI ACCTGC 1 cut(s) 995
Cac8I GCNNGC 5 cut(s) 560, 845, 2219, 2673, 2834
CciI TCATGA 1 cut(s) 2733
CfoI GCGC 4 cut(s) 1356, 1654, 2341, 3351
Cfr13I GGNCC 2 cut(s) 867, 2092
Cfr42I CCGCGG 1 cut(s) 2672
CseI GACGC 2 cut(s) 1907, 2201
CspCI CAANNNNNGTGG 2 cut(s) 648, 683
DraI TTTAAA 2 cut(s) 28, 987
DraIII CACNNNGTG 1 cut(s) 972
EaeI YGGCCR 1 cut(s) 3390
Eam1104I CTCTTC 3 cut(s) 198, 304, 630
EarI CTCTTC 3 cut(s) 198, 304, 630
Eco130I CCWWGG 4 cut(s) 762, 2085, 2259, 3082
Eco32I GATATC 2 cut(s) 413, 3277
Eco47I GGWCC 2 cut(s) 867, 2092
Eco57I CTGAAG 3 cut(s) 716, 827, 1503
Eco88I CYCGRG 2 cut(s) 2604, 3305
EcoRI GAATTC 1 cut(s) 1474
EcoRII CCWGG 8 cut(s) 208, 1179, 1676, 2144, 2386, 2413, 2790, 3392
EcoRV GATATC 2 cut(s) 413, 3277
EcoT14I CCWWGG 4 cut(s) 762, 2085, 2259, 3082
EcoT22I ATGCAT 2 cut(s) 153, 3231
ErhI CCWWGG 4 cut(s) 762, 2085, 2259, 3082
FalI AAGNNNNNCTT 2 cut(s) 1938, 1970
FaqI GGGAC 4 cut(s) 880, 1815, 3011, 3151
FauI CCCGC 1 cut(s) 2664
FauNDI CATATG 1 cut(s) 1930
FbaI TGATCA 2 cut(s) 1624, 2736
FblI GTMKAC 2 cut(s) 602, 1369
FokI GGATG 7 cut(s) 160, 564, 1158, 1247, 2068, 2497, 2855
FspBI CTAG 9 cut(s) 65, 1001, 1157, 2004, 2114, 2214, 2943, 3414, 3452
GlaI GCGC 4 cut(s) 1355, 1653, 2340, 3350
GsaI CCCAGC 1 cut(s) 1917
GsuI CTGGAG 3 cut(s) 591, 869, 1079
HaeIII GGCC 2 cut(s) 2626, 3392
HapII CCGG 4 cut(s) 83, 485, 1862, 2827
HgaI GACGC 2 cut(s) 1907, 2201
HhaI GCGC 4 cut(s) 1356, 1654, 2341, 3351
Hin1I GRCGYC 2 cut(s) 1918, 2193
Hin6I GCGC 4 cut(s) 1354, 1652, 2339, 3349
HinP1I GCGC 4 cut(s) 1354, 1652, 2339, 3349
HincII GTYRAC 2 cut(s) 603, 1504
HindII GTYRAC 2 cut(s) 603, 1504
HindIII AAGCTT 3 cut(s) 661, 841, 1394
HpaII CCGG 4 cut(s) 83, 485, 1862, 2827
HphI GGTGA 3 cut(s) 668, 2065, 3271
Hpy166II GTNNAC 7 cut(s) 328, 603, 676, 1370, 1504, 2697, 2788
Hpy8I GTNNAC 7 cut(s) 328, 603, 676, 1370, 1504, 2697, 2788
Hpy99I CGWCG 1 cut(s) 277
HpyAV CCTTC 9 cut(s) 782, 805, 821, 1136, 1792, 1961, 2681, 3280, 3455
HpyCH4III ACNGT 7 cut(s) 80, 432, 499, 902, 1128, 2348, 2892
HpyCH4IV ACGT 8 cut(s) 217, 920, 1054, 1416, 2063, 2360, 2779, 3468
HpyF10VI GCNNNNNNNGC 7 cut(s) 278, 1393, 1814, 2057, 2501, 2510, 2623
HpySE526I ACGT 8 cut(s) 217, 920, 1054, 1416, 2063, 2360, 2779, 3468
Hsp92I GRCGYC 2 cut(s) 1918, 2193
HspAI GCGC 4 cut(s) 1354, 1652, 2339, 3349
Kpn2I TCCGGA 1 cut(s) 82
Ksp22I TGATCA 2 cut(s) 1624, 2736
KspI CCGCGG 1 cut(s) 2672
LguI GCTCTTC 1 cut(s) 198
LmnI GCTCC 5 cut(s) 32, 555, 850, 1016, 3038
Lsp1109I GCAGC 7 cut(s) 1124, 1272, 1371, 1572, 2439, 2539, 3241
MaeI CTAG 9 cut(s) 65, 1001, 1157, 2004, 2114, 2214, 2943, 3414, 3452
MaeII ACGT 8 cut(s) 217, 920, 1054, 1416, 2063, 2360, 2779, 3468
MbiI CCGCTC 1 cut(s) 1547
MfeI CAATTG 5 cut(s) 894, 1071, 1379, 2900, 3104
MflI RGATCY 3 cut(s) 628, 925, 1864
MhlI GDGCHC 2 cut(s) 2914, 3413
MlsI TGGCCA 1 cut(s) 3392
MluNI TGGCCA 1 cut(s) 3392
MlyI GAGTC 5 cut(s) 598, 740, 1493, 2219, 2309
MmeI TCCRAC 4 cut(s) 242, 903, 1917, 2357
Mox20I TGGCCA 1 cut(s) 3392
Mph1103I ATGCAT 2 cut(s) 153, 3231
MroI TCCGGA 1 cut(s) 82
MroXI GAANNNNTTC 5 cut(s) 183, 246, 771, 2140, 3119
MscI TGGCCA 1 cut(s) 3392
MslI CAYNNNNRTG 5 cut(s) 333, 1215, 2236, 3249, 3438
Msp20I TGGCCA 1 cut(s) 3392
MspA1I CMGCKG 1 cut(s) 2671
MspCI CTTAAG 2 cut(s) 1273, 2612
MspI CCGG 4 cut(s) 83, 485, 1862, 2827
MspR9I CCNGG 8 cut(s) 210, 1181, 1678, 2146, 2388, 2415, 2792, 3394
MunI CAATTG 5 cut(s) 894, 1071, 1379, 2900, 3104
Mva1269I GAATGC 1 cut(s) 744
MvaI CCWGG 8 cut(s) 210, 1181, 1678, 2146, 2388, 2415, 2792, 3394
MvnI CGCG 3 cut(s) 1811, 2671, 3349
MwoI GCNNNNNNNGC 7 cut(s) 278, 1393, 1814, 2057, 2501, 2510, 2623
NcoI CCATGG 1 cut(s) 2085
NdeI CATATG 1 cut(s) 1930
NlaIV GGNNCC 6 cut(s) 624, 816, 868, 1029, 1866, 2911
NmuCI GTSAC 4 cut(s) 71, 1492, 2775, 3026
NsiI ATGCAT 2 cut(s) 153, 3231
NspI RCATGY 3 cut(s) 21, 562, 2836
NspV TTCGAA 1 cut(s) 1411
OliI CACNNNNGTG 1 cut(s) 333
PaeI GCATGC 2 cut(s) 562, 2836
PaeR7I CTCGAG 2 cut(s) 2604, 3305
PagI TCATGA 1 cut(s) 2733
PasI CCCWGGG 1 cut(s) 2387
PciSI GCTCTTC 1 cut(s) 198
PcsI WCGNNNNNNNCGW 1 cut(s) 2986
PctI GAATGC 1 cut(s) 744
PdmI GAANNNNTTC 5 cut(s) 183, 246, 771, 2140, 3119
PfeI GAWTC 8 cut(s) 767, 1036, 1120, 1538, 1574, 2680, 2846, 2939
PfoI TCCNGGA 1 cut(s) 2144
PleI GAGTC 5 cut(s) 598, 739, 1492, 2218, 2308
PpsI GAGTC 5 cut(s) 598, 739, 1492, 2218, 2308
Ppu21I YACGTR 1 cut(s) 2780
PshAI GACNNNNGTC 3 cut(s) 78, 1499, 3012
PshBI ATTAAT 1 cut(s) 573
Psp6I CCWGG 8 cut(s) 208, 1179, 1676, 2144, 2386, 2413, 2790, 3392
PspFI CCCAGC 1 cut(s) 1913
PspGI CCWGG 8 cut(s) 208, 1179, 1676, 2144, 2386, 2413, 2790, 3392
PspN4I GGNNCC 6 cut(s) 624, 816, 868, 1029, 1866, 2911
PspPI GGNCC 2 cut(s) 867, 2092
PspXI VCTCGAGB 1 cut(s) 3305
PstI CTGCAG 1 cut(s) 3097
PsuI RGATCY 3 cut(s) 628, 925, 1864
RseI CAYNNNNRTG 5 cut(s) 333, 1215, 2236, 3249, 3438
SacII CCGCGG 1 cut(s) 2672
SalI GTCGAC 1 cut(s) 601
SapI GCTCTTC 1 cut(s) 198
Sau96I GGNCC 2 cut(s) 867, 2092
ScaI AGTACT 3 cut(s) 2018, 2610, 3202
SchI GAGTC 5 cut(s) 598, 740, 1493, 2219, 2309
ScrFI CCNGG 8 cut(s) 210, 1181, 1678, 2146, 2388, 2415, 2792, 3394
SduI GDGCHC 2 cut(s) 2914, 3413
SfcI CTRYAG 4 cut(s) 1124, 1578, 1704, 3093
Sfr274I CTCGAG 2 cut(s) 2604, 3305
Sfr303I CCGCGG 1 cut(s) 2672
SfuI TTCGAA 1 cut(s) 1411
SgrBI CCGCGG 1 cut(s) 2672
SinI GGWCC 2 cut(s) 867, 2092
SlaI CTCGAG 2 cut(s) 2604, 3305
SmiMI CAYNNNNRTG 5 cut(s) 333, 1215, 2236, 3249, 3438
SmlI CTYRAG 8 cut(s) 331, 374, 1273, 2297, 2321, 2604, 2612, 3305
SmoI CTYRAG 8 cut(s) 331, 374, 1273, 2297, 2321, 2604, 2612, 3305
SphI GCATGC 2 cut(s) 562, 2836
SsiI CCGC 8 cut(s) 520, 1547, 1809, 2051, 2196, 2669, 2671, 3347
SspI AATATT 1 cut(s) 780
SspMI CTAG 9 cut(s) 65, 1001, 1157, 2004, 2114, 2214, 2943, 3414, 3452
StyD4I CCNGG 8 cut(s) 208, 1179, 1676, 2144, 2386, 2413, 2790, 3392
StyI CCWWGG 4 cut(s) 762, 2085, 2259, 3082
TaaI ACNGT 7 cut(s) 80, 432, 499, 902, 1128, 2348, 2892
TaiI ACGT 8 cut(s) 220, 923, 1057, 1419, 2066, 2363, 2782, 3471
TaqII GACCGA 1 cut(s) 1796
TatI WGTACW 5 cut(s) 253, 1433, 2016, 2608, 3200
TauI GCSGC 3 cut(s) 522, 1811, 2198
TfiI GAWTC 8 cut(s) 767, 1036, 1120, 1538, 1574, 2680, 2846, 2939
TscAI CASTG 6 cut(s) 622, 973, 1446, 1644, 2746, 2958
TseFI GTSAC 4 cut(s) 71, 1492, 2775, 3026
TseI GCWGC 7 cut(s) 1137, 1260, 1384, 1585, 2452, 2527, 3254
Tsp45I GTSAC 4 cut(s) 71, 1492, 2775, 3026
TspGWI ACGGA 3 cut(s) 986, 1389, 1460
TspRI CASTG 6 cut(s) 622, 973, 1446, 1644, 2746, 2958
Vha464I CTTAAG 2 cut(s) 1273, 2612
VpaK11BI GGWCC 2 cut(s) 867, 2092
VspI ATTAAT 1 cut(s) 573
XapI RAATTY 9 cut(s) 1242, 1474, 1684, 1713, 1871, 2246, 2366, 2585, 3045
XbaI TCTAGA 1 cut(s) 2942
XceI RCATGY 3 cut(s) 21, 562, 2836
XcmI CCANNNNNNNNNTGG 1 cut(s) 2083
XhoI CTCGAG 2 cut(s) 2604, 3305
XmiI GTMKAC 2 cut(s) 602, 1369
XmnI GAANNNNTTC 5 cut(s) 183, 246, 771, 2140, 3119
XspI CTAG 9 cut(s) 65, 1001, 1157, 2004, 2114, 2214, 2943, 3414, 3452
ZrmI AGTACT 3 cut(s) 2018, 2610, 3202
Zsp2I ATGCAT 2 cut(s) 153, 3231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.