MD05G1002300.v1.1

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000129
Physical Location & Seq
Forward (+)
964110 .. 966940
2831 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1002300.v1.1.491

Sequence Viewer

Length: 2259 bp
ATGAAAGTGATGGGGCAGACAATACTCCCAGTTTTCTATGACGTGGATCCATCTGAGGTACGAAAACAAATGGGGAATTTCAGGAAAGCCTTCTCCAAGCATGAAGAAGCCTTTAAGGATAACACAGAGAAGGTGCGTAAGTGGAGAGATGCACTTACTGAAGTAGCTGATCTCTATGGATGGCATTTACAAGATGGATATGAGTCGAAAATTATTGAAGATATTGTGAGATATATTTTCACTAAACTAAATCAAACAATTTCAAGTATACATATGGATTTAGTTGGGATGGATTCACGCGTAAACGAAGTGATTTCATGCCTAGACACAGGGTTGAACAATGTATGCATGTTAGGGATTTGGGGGATGGGGGGCATGGGCAAGACAACCATTGCAGAAGTAGTTTTTGATAGGATACGTTCTCGGTTTGATGCTTACAGCTTTCTTGCCAATGTTAGAGAGGTAACTGAGAAACAAGGTCTAGTCCATTTACATAAGCAACTTCTTTCAGATATCCTGTTCGAGAGTAGTGTAGATGTACATAATATCCATATGGGGATCAATAAGATAAGACAGAGACTGCGTACTAGAATGGTTCTTATCATTCTTGATGATGTGGATCAATTAGAACAGTTGGAAGCATTGTGTGGTCATAGTTGGTTTGGTTCGGGGAGTAGGATCATTATAACATCAAGGGATGAGCACTTGCTATGCACATATGGAGTGGATAAAATGTATAAGGTTAAGCCGTTAACTGATGATGAAGCTCTTCAGCTCTTTTGTCGGAAAGCATTCAAGAAAGACCAGGTTGGAGAAGACTTTCTCGAGCTATCAAAGAATGTTGTGGAATACGCTAATGGCCTCCCATTAGCTATTGAAGTGTTGGGTTCATTCCTCTTTGGTAGAAGCGTAGAAGTATGGTCAAGTGCTTTGGATAGACTTAAAGAAAATCCTGACAGAAGAATTACTGATGTGCTTAAAGTAAGTTTTGATGCTTTACAAGACGTAGAAAAGAAAGTATTTTTGGACATTGCATGTTTCTTTAAAGGGGAGGATAAAGATCGAGTTACAAGAATACTGGAAAGTAGTTGTGGCTATCGTCCAGACATTGATATAGCAGTTCTCATGGAAAAATCTCTACTAACTCTATTTGGAAGAAAATTGTGGATGCATGATTTGATTCAAGAATTAGGTTGGGAGATTGTTCGTCGTGAATGCTATGAAGAGCCGGGCAAACGTAGCAGGCTATGGCTTCCCAAGGATATCATCCATGTATTTGTAAACAATGAGGGAACACTTGCAGTGGAAGGTATATTCTTGAATCTACCAAAACAAGAGGTGGTTCACGTAAATGTTGACCCGTTCTCAAAGATGTGCAAGCTAAGATTGTTAAAAATTAGTAATGTGCACTTTCCTGGATCTATCAGATACCTTTCTAACGAGTTACAACTGCTGGAATGGCATGCATTTCCTTTTAATTCTTTGCCATCAAACTTTCAATCAGATAAGCTAGTGGAACTCCGCATGCATTCAAGCCGTATCAAACAAGTATGGAGGGGAAATAAACGCTGGAGTATGCTACAGTTTATCGATCTAAGTGATTCCGTGTACTTGATTGCAACCCCAAACTTCGTTGAGGTTCCAAATCTTGAGAAGTTGGTGCTTCAAGGTTGTACAAGATTGGTTGAGGTTCACCCATCCCTTGGATTTCTCAAAAACCTTATCTTGTTGAATATGAGAAACTGCAAATCCATTGAGAGCCTTCCACCTTTCATCAGTTTGGAATCTCTTAAAACTTTAACTCTCTCTGGTTCTTCAAGACTCAAGAGGTTTCCTGAAATTGAAGGAAATATGAAAAGCTTGTTGGAGCTTCATCTAGATGGAACTGCCATTGAAGATTTACCTCCATCAATTCAACGTTTGACTGGTCTTACATTGATGAATTTGGGAGGCTGCAGAAACCTTTTCCGTCTTCCAAGTACCATTCAGTATTTGACGTCTCTGAAAACTCTCATTCTAACAGGTTGCTCAGAACTTGATGACATACCTGAGGATTTGAATTGTGTGGAATATCTTGAGGACCTTGATATAAGTGGAACTGCTATAAGAGAATCATCATCCATTGCAGCTATGAAAAATCTGAAGACTCTATCCTTCCAAGGATGTAAAGATCAACCTTCAAAATCGTGGCATTCTCTCTTTACTTGTTGGTGGTGGGGTAGAAAGGGAAATGTTCCTGACATTAGACTTGAGTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

753

Amino Acids

86.25

Weight (kDa)

6.35

Isoelectric Point (pI)

46.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 3 - 89 9.5e-19 TIR domain
NB-ARC PF00931 99 - 268 1.4e-22 NB-ARC domain
NACHT PF05729 121 - 266 7.6e-07 NACHT domain
WHD_ROQ1 PF23282 337 - 404 2.3e-15 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 563 - 718 2.4e-11 Leucine-rich repeat region
LRR_13 PF23286 594 - 680 2.2e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000129)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_2g02050 FvH4_5g38500 FvH4_5g38520 FvH4_5g38540 FvH4_5g38540 FvH4_5g38540 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38561 FvH4_5g38600 FvH4_5g38620 FvH4_5g38630 FvH4_5g38660 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38680 FvH4_5g38760
malus_domestica MD05G1002100.v1.1 MD05G1002200.v1.1 MD05G1002300.v1.1 MD05G1002400.v1.1 MD07G1123900.v1.1 MD17G1192900.v1.1
prunus_persica Prupe.2G059400_v2.0.a1 Prupe.8G005400_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005500_v2.0.a1 Prupe.8G005600_v2.0.a1 Prupe.8G102900_v2.0.a1
pyrus_communis pycom05g00210 pycom05g00220 pycom05g00230 pycom07g11690
rosa_chinensis RchiOBHm_Chr6g0250791 RchiOBHm_Chr7g0223661 RchiOBHm_Chr7g0240571 RchiOBHm_Chr7g0240581 RchiOBHm_Chr7g0240591 RchiOBHm_Chr7g0240641 RchiOBHm_Chr7g0240681
rosa_laevigata RLG00000000629 RLG00000000630 RLG00000000632 RLG00000000636 RLG00000000637 RLG00000000638 RLG00000000639 RLG00000000640 RLG00000000648 RLG00000000657 RLG00000000661 RLG00000000664 RLG00000000666 RLG00000000669 RLG00000000672 RLG00000000673 RLG00000001954 RLG00000001970 RLG00000007791 RLG00000012997 RLG00000018666
rosa_multiflora Rmu_co8160218.1_g000001 Rmu_co8184794.1_g000001 Rmu_co8275571.1_g000001 Rmu_co8344485.1_g000001 Rmu_co8381261.1_g000001 Rmu_co8395583.1_g000001 Rmu_sc0001070.1_g000010 Rmu_sc0001070.1_g000016 Rmu_sc0001070.1_g000024 Rmu_sc0001070.1_g000025 Rmu_sc0001070.1_g000031 Rmu_sc0001209.1_g000012 Rmu_sc0001483.1_g000003 Rmu_sc0001483.1_g000013 Rmu_sc0001483.1_g000015 Rmu_sc0001921.1_g000013 Rmu_sc0001921.1_g000015 Rmu_sc0001921.1_g000019 Rmu_sc0001921.1_g000027 Rmu_sc0001921.1_g000028 Rmu_sc0001921.1_g000030 Rmu_sc0001924.1_g000001 Rmu_sc0001924.1_g000004 Rmu_sc0002517.1_g000003 Rmu_sc0002517.1_g000014 Rmu_sc0002551.1_g000001 Rmu_sc0002551.1_g000010 Rmu_sc0002551.1_g000031 Rmu_sc0002551.1_g000039 Rmu_sc0002551.1_g000040 Rmu_sc0004799.1_g000007 Rmu_sc0005071.1_g000006 Rmu_sc0005071.1_g000013 Rmu_sc0005071.1_g000019 Rmu_sc0005327.1_g000001 Rmu_sc0005327.1_g000002 Rmu_sc0009028.1_g000001 Rmu_sc0013246.1_g000001 Rmu_sc0014401.1_g000001 Rmu_sc0019569.1_g000001 Rmu_sc0019569.1_g000003 Rmu_sc0019569.1_g000004 Rmu_sc0020672.1_g000001 Rmu_sc0023716.1_g000001 Rmu_sc0037115.1_g000001 Rmu_sc0038364.1_g000001 Rmu_ssc0000011.1_g000037
rosa_roxburghii Rroxscaffold_2G00121840 Rroxscaffold_2G00121850 Rroxscaffold_2G00121870 Rroxscaffold_3G00219670 Rroxscaffold_3G00219690 Rroxscaffold_3G00219710 Rroxscaffold_3G00219770 Rroxscaffold_3G00219810 Rroxscaffold_3G00219820 Rroxscaffold_3G00219850 Rroxscaffold_3G00219870 Rroxscaffold_3G00231380 Rroxscaffold_3G00235440 Rroxscaffold_3G00236130 Rroxscaffold_3G00236140 Rroxscaffold_3G00236270 Rroxscaffold_3G00236280 Rroxscaffold_3G00240810
rosa_rugosa Rorug02G0237100 Rorug07G0218500 Rorug07G0218600 Rorug07G0218600 Rorug07G0218700 Rorug07G0323600 Rorug07G0337100 Rorug07G0337200 Rorug07G0337500 Rorug07G0337700 Rorug07G0337800 Rorug07G0337800 Rorug07G0338000 Rorug07G0338100 Rorug07G0338200 Rorug07G0338300
rosa_samantha Rh1BG151400 Rh2CG282500 Rh2DG318800 Rh2DG318900 Rh2DG319100 Rh5BG508500 Rh6BG254000 Rh6DG245200 Rh7AG361400 Rh7AG491500 Rh7AG492000 Rh7AG492300 Rh7AG492400 Rh7AG492500 Rh7AG492800 Rh7BG464100 Rh7BG464500 Rh7BG464700 Rh7BG464800 Rh7CG379900 Rh7CG507500 Rh7CG508000 Rh7CG508700 Rh7CG508900 Rh7CG509900 Rh7CG510400 Rh7CG510500 Rh7CG510600 Rh7CG510700 Rh7CG511000 Rh7CG511200
rosa_wichuraiana Rw0G005770 Rw7G041180 Rw7G041190 Rw7G041250 Rw7G041270 Rw7G041280 Rw7G041300 Rw7G041330 Rw7G041410 Rw7G041420 Rw7G041440 Rw7G041460 Rw7G041470 Rw7G041480 Rw7G041500 Rw7G041530 Rw7G041540 Rw7G041570 Rw7G041580 Rw7G041600 Rw7G041630 Rw7G041660 Rw7G041680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 686
AatII GACGTC 1 cut(s) 2000
AccB7I CCANNNNNTGG 1 cut(s) 1703
AccI GTMKAC 1 cut(s) 268
AccII CGCG 1 cut(s) 300
AciI CCGC 1 cut(s) 1522
AclI AACGTT 1 cut(s) 1918
AclWI GGATC 6 cut(s) 41, 54, 566, 627, 686, 1426
AcsI RAATTY 2 cut(s) 76, 1942
AcuI CTGAAG 3 cut(s) 180, 755, 2162
AcyI GRCGYC 1 cut(s) 1997
AfaI GTAC 6 cut(s) 60, 540, 586, 1610, 1675, 1981
AfiI CCNNNNNNNGG 1 cut(s) 1703
AflIII ACRYGT 1 cut(s) 298
AjiI CACGTC 1 cut(s) 43
AjnI CCWGG 2 cut(s) 804, 1414
AjuI GAANNNNNNNTTGG 4 cut(s) 1007, 1039, 1847, 1879
Alw21I GWGCWC 2 cut(s) 705, 1410
Alw26I GTCTC 2 cut(s) 571, 2004
Alw44I GTGCAC 1 cut(s) 1406
AlwI GGATC 6 cut(s) 41, 54, 566, 627, 686, 1426
AlwNI CAGNNNCTG 1 cut(s) 580
Ama87I CYCGRG 1 cut(s) 824
AoxI GGCC 1 cut(s) 859
ApaLI GTGCAC 1 cut(s) 1406
ApeKI GCWGC 2 cut(s) 1953, 2126
ApoI RAATTY 2 cut(s) 76, 1942
Asp700I GAANNNNTTC 4 cut(s) 89, 768, 791, 819
AspS9I GGNCC 1 cut(s) 2080
AsuC2I CCSGG 1 cut(s) 1230
AsuHPI GGTGA 1 cut(s) 1685
AvaI CYCGRG 1 cut(s) 824
AvaII GGWCC 1 cut(s) 2080
AxyI CCTNAGG 1 cut(s) 2049
BaeGI GKGCMC 1 cut(s) 1410
BamHI GGATCC 1 cut(s) 46
BbsI GAAGAC 3 cut(s) 822, 1964, 2150
Bbv12I GWGCWC 2 cut(s) 705, 1410
BbvI GCAGC 2 cut(s) 1940, 2138
BceAI ACGGC 2 cut(s) 733, 1521
BciT130I CCWGG 2 cut(s) 806, 1416
BciVI GTATCC 1 cut(s) 408
BcnI CCSGG 1 cut(s) 1230
BcoDI GTCTC 2 cut(s) 571, 2004
BfaI CTAG 5 cut(s) 323, 482, 588, 1511, 1877
BfmI CTRYAG 2 cut(s) 1580, 1954
BfuI GTATCC 1 cut(s) 408
BisI GCNGC 2 cut(s) 1954, 2127
BlsI GCNGC 2 cut(s) 1955, 2128
Bme1390I CCNGG 3 cut(s) 806, 1230, 1416
Bme18I GGWCC 1 cut(s) 2080
BmeT110I CYCGRG 1 cut(s) 824
BmgBI CACGTC 1 cut(s) 43
BmgT120I GGNCC 1 cut(s) 2080
BmiI GGNNCC 2 cut(s) 48, 1641
BmrFI CCNGG 3 cut(s) 806, 1230, 1416
BmrI ACTGGG 1 cut(s) 23
BmsI GCATC 4 cut(s) 139, 421, 982, 1158
BmuI ACTGGG 1 cut(s) 23
BpiI GAAGAC 3 cut(s) 822, 1964, 2150
BpmI CTGGAG 1 cut(s) 1591
BpuEI CTTGAG 3 cut(s) 1670, 1808, 2096
BpuMI CCSGG 1 cut(s) 1230
Bsa29I ATCGAT 1 cut(s) 1590
BsaAI YACGTR 1 cut(s) 1348
BsaBI GATNNNNATC 2 cut(s) 618, 1059
BsaHI GRCGYC 1 cut(s) 1997
BsaJI CCNNGG 3 cut(s) 1257, 1702, 2158
Bsc4I CCNNNNNNNGG 1 cut(s) 1703
Bse1I ACTGG 3 cut(s) 29, 1083, 1930
Bse21I CCTNAGG 1 cut(s) 2049
Bse3DI GCAATG 3 cut(s) 390, 1029, 2121
Bse8I GATNNNNATC 2 cut(s) 618, 1059
BseBI CCWGG 2 cut(s) 806, 1416
BseCI ATCGAT 1 cut(s) 1590
BseDI CCNNGG 3 cut(s) 1257, 1702, 2158
BseGI GGATG 9 cut(s) 185, 294, 372, 703, 1173, 1266, 1697, 2117, 2168
BseJI GATNNNNATC 2 cut(s) 618, 1059
BseLI CCNNNNNNNGG 1 cut(s) 1703
BseMI GCAATG 3 cut(s) 390, 1029, 2121
BseMII CTCAG 4 cut(s) 45, 459, 2040, 2043
BseNI ACTGG 3 cut(s) 29, 1083, 1930
BseSI GKGCMC 1 cut(s) 1410
BseXI GCAGC 2 cut(s) 1940, 2138
Bsh1236I CGCG 1 cut(s) 300
BshFI GGCC 1 cut(s) 861
BshVI ATCGAT 1 cut(s) 1590
BsiHKAI GWGCWC 2 cut(s) 705, 1410
BsiHKCI CYCGRG 1 cut(s) 824
BsiSI CCGG 1 cut(s) 1229
BslI CCNNNNNNNGG 1 cut(s) 1703
BsmAI GTCTC 2 cut(s) 571, 2004
BsmBI CGTCTC 1 cut(s) 2004
BsmI GAATGC 4 cut(s) 791, 1220, 1528, 2191
BsnI GGCC 1 cut(s) 861
BsoBI CYCGRG 1 cut(s) 824
Bsp1286I GDGCHC 2 cut(s) 705, 1410
Bsp1407I TGTACA 2 cut(s) 538, 1673
Bsp143I GATC 9 cut(s) 46, 169, 558, 619, 678, 1060, 1418, 1591, 2170
BspACI CCGC 1 cut(s) 1522
BspANI GGCC 1 cut(s) 861
BspCNI CTCAG 4 cut(s) 46, 460, 2041, 2042
BspDI ATCGAT 1 cut(s) 1590
BspFNI CGCG 1 cut(s) 300
BspLI GGNNCC 2 cut(s) 48, 1641
BspMAI CTGCAG 1 cut(s) 1958
BspPI GGATC 6 cut(s) 41, 54, 566, 627, 686, 1426
BspQI GCTCTTC 2 cut(s) 774, 1218
BsrDI GCAATG 3 cut(s) 390, 1029, 2121
BsrGI TGTACA 2 cut(s) 538, 1673
BsrI ACTGG 3 cut(s) 29, 1083, 1930
BssECI CCNNGG 3 cut(s) 1257, 1702, 2158
BssMI GATC 9 cut(s) 46, 169, 558, 619, 678, 1060, 1418, 1591, 2170
BssNAI GTATAC 1 cut(s) 269
BssNI GRCGYC 1 cut(s) 1997
BssT1I CCWWGG 3 cut(s) 1257, 1702, 2158
Bst1107I GTATAC 1 cut(s) 269
Bst2UI CCWGG 2 cut(s) 806, 1416
Bst4CI ACNGT 2 cut(s) 633, 1584
Bst6I CTCTTC 2 cut(s) 774, 1218
BstACI GRCGYC 1 cut(s) 1997
BstAUI TGTACA 2 cut(s) 538, 1673
BstBAI YACGTR 1 cut(s) 1348
BstC8I GCNNGC 4 cut(s) 1244, 1379, 1464, 1526
BstDEI CTNAG 6 cut(s) 54, 468, 1382, 1595, 2029, 2049
BstF5I GGATG 9 cut(s) 185, 294, 372, 703, 1173, 1266, 1697, 2117, 2168
BstFNI CGCG 1 cut(s) 300
BstKTI GATC 9 cut(s) 49, 172, 561, 622, 681, 1063, 1421, 1594, 2173
BstMAI GTCTC 2 cut(s) 571, 2004
BstMBI GATC 9 cut(s) 46, 169, 558, 619, 678, 1060, 1418, 1591, 2170
BstMWI GCNNNNNNNGC 2 cut(s) 1239, 1459
BstNI CCWGG 2 cut(s) 806, 1416
BstNSI RCATGY 4 cut(s) 352, 1038, 1466, 1528
BstSCI CCNGG 3 cut(s) 804, 1228, 1414
BstSFI CTRYAG 2 cut(s) 1580, 1954
BstSLI GKGCMC 1 cut(s) 1410
BstUI CGCG 1 cut(s) 300
BstV1I GCAGC 2 cut(s) 1940, 2138
BstV2I GAAGAC 3 cut(s) 822, 1964, 2150
BstX2I RGATCY 2 cut(s) 46, 1418
BstYI RGATCY 2 cut(s) 46, 1418
BstZ17I GTATAC 1 cut(s) 269
Bsu15I ATCGAT 1 cut(s) 1590
Bsu36I CCTNAGG 1 cut(s) 2049
BsuI GTATCC 1 cut(s) 408
BsuRI GGCC 1 cut(s) 861
BsuTUI ATCGAT 1 cut(s) 1590
BtrI CACGTC 1 cut(s) 43
BtsCI GGATG 9 cut(s) 185, 294, 372, 703, 1173, 1266, 1697, 2117, 2168
BtsI GCAGTG 1 cut(s) 1308
BtsIMutI CAGTG 1 cut(s) 1308
Cac8I GCNNGC 4 cut(s) 1244, 1379, 1464, 1526
CaiI CAGNNNCTG 1 cut(s) 580
Cfr13I GGNCC 1 cut(s) 2080
ClaI ATCGAT 1 cut(s) 1590
CsiI ACCWGGT 1 cut(s) 804
Csp6I GTAC 6 cut(s) 59, 539, 585, 1609, 1674, 1980
CviQI GTAC 6 cut(s) 59, 539, 585, 1609, 1674, 1980
DdeI CTNAG 6 cut(s) 54, 468, 1382, 1595, 2029, 2049
DpnI GATC 9 cut(s) 48, 171, 560, 621, 680, 1062, 1420, 1593, 2172
DpnII GATC 9 cut(s) 46, 169, 558, 619, 678, 1060, 1418, 1591, 2170
DraI TTTAAA 1 cut(s) 1045
Eam1104I CTCTTC 2 cut(s) 774, 1218
EarI CTCTTC 2 cut(s) 774, 1218
Eco130I CCWWGG 3 cut(s) 1257, 1702, 2158
Eco32I GATATC 2 cut(s) 514, 1264
Eco47I GGWCC 1 cut(s) 2080
Eco57I CTGAAG 3 cut(s) 180, 755, 2162
Eco81I CCTNAGG 1 cut(s) 2049
Eco88I CYCGRG 1 cut(s) 824
EcoO109I RGGNCCY 1 cut(s) 2080
EcoRII CCWGG 2 cut(s) 804, 1414
EcoRV GATATC 2 cut(s) 514, 1264
EcoT14I CCWWGG 3 cut(s) 1257, 1702, 2158
EcoT22I ATGCAT 4 cut(s) 350, 1173, 1468, 1530
ErhI CCWWGG 3 cut(s) 1257, 1702, 2158
Esp3I CGTCTC 1 cut(s) 2004
FauNDI CATATG 3 cut(s) 273, 552, 718
FblI GTMKAC 1 cut(s) 268
Fnu4HI GCNGC 2 cut(s) 1954, 2127
FokI GGATG 9 cut(s) 192, 301, 379, 710, 1180, 1253, 1684, 2104, 2175
Fsp4HI GCNGC 2 cut(s) 1954, 2127
FspBI CTAG 5 cut(s) 323, 482, 588, 1511, 1877
GluI GCNGC 2 cut(s) 1954, 2127
GsuI CTGGAG 1 cut(s) 1591
HaeIII GGCC 1 cut(s) 861
HapII CCGG 1 cut(s) 1229
Hin1I GRCGYC 1 cut(s) 1997
HincII GTYRAC 2 cut(s) 753, 1357
HindII GTYRAC 2 cut(s) 753, 1357
HindIII AAGCTT 1 cut(s) 1858
HinfI GANTC 9 cut(s) 203, 293, 1180, 1321, 1601, 1784, 1821, 2111, 2146
HpaI GTTAAC 1 cut(s) 753
HpaII CCGG 1 cut(s) 1229
HphI GGTGA 1 cut(s) 1685
Hpy166II GTNNAC 9 cut(s) 269, 304, 753, 1282, 1345, 1357, 1408, 1609, 1693
Hpy188I TCNGA 8 cut(s) 55, 511, 786, 1427, 1504, 2004, 2032, 2142
Hpy8I GTNNAC 9 cut(s) 269, 304, 753, 1282, 1345, 1357, 1408, 1609, 1693
Hpy99I CGWCG 1 cut(s) 1212
HpyAV CCTTC 7 cut(s) 100, 124, 1301, 1772, 1838, 2164, 2187
HpyCH4III ACNGT 2 cut(s) 633, 1584
HpyCH4IV ACGT 7 cut(s) 42, 418, 1005, 1237, 1347, 1918, 1997
HpyF10VI GCNNNNNNNGC 2 cut(s) 1239, 1459
HpyF3I CTNAG 6 cut(s) 54, 468, 1382, 1595, 2029, 2049
HpySE526I ACGT 7 cut(s) 42, 418, 1005, 1237, 1347, 1918, 1997
Hsp92I GRCGYC 1 cut(s) 1997
KspAI GTTAAC 1 cut(s) 753
Kzo9I GATC 9 cut(s) 46, 169, 558, 619, 678, 1060, 1418, 1591, 2170
LguI GCTCTTC 2 cut(s) 774, 1218
LmnI GCTCC 1 cut(s) 1867
Lsp1109I GCAGC 2 cut(s) 1940, 2138
LweI GCATC 4 cut(s) 139, 421, 982, 1158
MabI ACCWGGT 1 cut(s) 804
MaeI CTAG 5 cut(s) 323, 482, 588, 1511, 1877
MaeII ACGT 7 cut(s) 42, 418, 1005, 1237, 1347, 1918, 1997
MaeIII GTNAC 3 cut(s) 463, 1066, 1443
MalI GATC 9 cut(s) 48, 171, 560, 621, 680, 1062, 1420, 1593, 2172
MboI GATC 9 cut(s) 46, 169, 558, 619, 678, 1060, 1418, 1591, 2170
MflI RGATCY 2 cut(s) 46, 1418
MhlI GDGCHC 2 cut(s) 705, 1410
MluI ACGCGT 1 cut(s) 298
MlyI GAGTC 3 cut(s) 212, 1815, 2140
MmeI TCCRAC 4 cut(s) 615, 764, 790, 1845
Mph1103I ATGCAT 4 cut(s) 350, 1173, 1468, 1530
MroXI GAANNNNTTC 4 cut(s) 89, 768, 791, 819
MslI CAYNNNNRTG 2 cut(s) 1350, 1878
MspI CCGG 1 cut(s) 1229
MspR9I CCNGG 3 cut(s) 806, 1230, 1416
Mva1269I GAATGC 4 cut(s) 791, 1220, 1528, 2191
MvaI CCWGG 2 cut(s) 806, 1416
MvnI CGCG 1 cut(s) 300
MwoI GCNNNNNNNGC 2 cut(s) 1239, 1459
NciI CCSGG 1 cut(s) 1230
NdeI CATATG 3 cut(s) 273, 552, 718
NdeII GATC 9 cut(s) 46, 169, 558, 619, 678, 1060, 1418, 1591, 2170
NlaIV GGNNCC 2 cut(s) 48, 1641
NsiI ATGCAT 4 cut(s) 350, 1173, 1468, 1530
NspI RCATGY 4 cut(s) 352, 1038, 1466, 1528
PaeI GCATGC 2 cut(s) 1466, 1528
PaeR7I CTCGAG 1 cut(s) 824
PciSI GCTCTTC 2 cut(s) 774, 1218
PctI GAATGC 4 cut(s) 791, 1220, 1528, 2191
PdmI GAANNNNTTC 4 cut(s) 89, 768, 791, 819
PfeI GAWTC 6 cut(s) 293, 1180, 1321, 1601, 1784, 2111
PflMI CCANNNNNTGG 1 cut(s) 1703
PfoI TCCNGGA 1 cut(s) 1414
PkrI GCNGC 2 cut(s) 1955, 2128
PleI GAGTC 3 cut(s) 211, 1815, 2140
PpsI GAGTC 3 cut(s) 211, 1815, 2140
Ppu21I YACGTR 1 cut(s) 1348
PpuMI RGGWCCY 1 cut(s) 2080
PsiI TTATAA 1 cut(s) 686
Psp1406I AACGTT 1 cut(s) 1918
Psp5II RGGWCCY 1 cut(s) 2080
Psp6I CCWGG 2 cut(s) 804, 1414
PspGI CCWGG 2 cut(s) 804, 1414
PspN4I GGNNCC 2 cut(s) 48, 1641
PspPI GGNCC 1 cut(s) 2080
PspPPI RGGWCCY 1 cut(s) 2080
PstI CTGCAG 1 cut(s) 1958
PstNI CAGNNNCTG 1 cut(s) 580
PsuI RGATCY 2 cut(s) 46, 1418
RsaI GTAC 6 cut(s) 60, 540, 586, 1610, 1675, 1981
RsaNI GTAC 6 cut(s) 59, 539, 585, 1609, 1674, 1980
RseI CAYNNNNRTG 2 cut(s) 1350, 1878
SapI GCTCTTC 2 cut(s) 774, 1218
SatI GCNGC 2 cut(s) 1954, 2127
Sau3AI GATC 9 cut(s) 46, 169, 558, 619, 678, 1060, 1418, 1591, 2170
Sau96I GGNCC 1 cut(s) 2080
SchI GAGTC 3 cut(s) 212, 1815, 2140
ScrFI CCNGG 3 cut(s) 806, 1230, 1416
SduI GDGCHC 2 cut(s) 705, 1410
SexAI ACCWGGT 1 cut(s) 804
SfaNI GCATC 4 cut(s) 139, 421, 982, 1158
SfcI CTRYAG 2 cut(s) 1580, 1954
Sfr274I CTCGAG 1 cut(s) 824
SinI GGWCC 1 cut(s) 2080
SlaI CTCGAG 1 cut(s) 824
SmiMI CAYNNNNRTG 2 cut(s) 1350, 1878
SmlI CTYRAG 5 cut(s) 824, 1649, 1823, 2075, 2249
SmoI CTYRAG 5 cut(s) 824, 1649, 1823, 2075, 2249
SphI GCATGC 2 cut(s) 1466, 1528
SsiI CCGC 1 cut(s) 1522
SspMI CTAG 5 cut(s) 323, 482, 588, 1511, 1877
StyD4I CCNGG 3 cut(s) 804, 1228, 1414
StyI CCWWGG 3 cut(s) 1257, 1702, 2158
TaaI ACNGT 2 cut(s) 633, 1584
TaiI ACGT 7 cut(s) 45, 421, 1008, 1240, 1350, 1921, 2000
TaqI TCGA 5 cut(s) 206, 522, 825, 1063, 1590
TatI WGTACW 3 cut(s) 538, 1608, 1673
TfiI GAWTC 6 cut(s) 293, 1180, 1321, 1601, 1784, 2111
TscAI CASTG 1 cut(s) 1308
TseI GCWGC 2 cut(s) 1953, 2126
TspGWI ACGGA 2 cut(s) 1594, 1958
TspRI CASTG 1 cut(s) 1308
Van91I CCANNNNNTGG 1 cut(s) 1703
VneI GTGCAC 1 cut(s) 1406
VpaK11BI GGWCC 1 cut(s) 2080
XapI RAATTY 2 cut(s) 76, 1942
XbaI TCTAGA 1 cut(s) 1876
XceI RCATGY 4 cut(s) 352, 1038, 1466, 1528
XhoI CTCGAG 1 cut(s) 824
XmiI GTMKAC 1 cut(s) 268
XmnI GAANNNNTTC 4 cut(s) 89, 768, 791, 819
XspI CTAG 5 cut(s) 323, 482, 588, 1511, 1877
ZraI GACGTC 1 cut(s) 1998
Zsp2I ATGCAT 4 cut(s) 350, 1173, 1468, 1530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.