FvH4_6g26861

Zinc knuckle

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000123
Physical Location & Seq
Reverse (-)
20548710 .. 20552152
3443 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g26861.t1

Sequence Viewer

Length: 696 bp
ATGCAGGTCCATTGGTTATGCACCTTTCTTTCTTGCAAAACCCTAGCCGGCTCGAGAACGCCGTCGACAGGAATGAGACTGTCCGACTGCACCATAGTGTTGGAAATGACCTTTGGCCTTGCCAGTGAAGGGTTGTGTCGTGGCTGTGTTATGGGATCTGCAGGGGGTTGCAAACTTGCCGGATCAGGGCGAAGTCGGCTGACAGACAGAGGCCGGCGGTTGAAGGTTGCTGAGGAAAACGGCAGCGCTACCTCCAAGCTCGGAGGAAGCGGTGGTTACTCTAGAGGGGGACTAGATCTCATCCGAGACTGGTGGGTTTCTTTTTGTGCAGCCGGCTTTGGCTATTTTGTCCGCCCTGGTGGCAGTATGGAGGGTGAAAGGGATGGTCGTCCAGAGGAGGTCGGATTCTTTGTTTTCTGCTTCAAGGAGGCAGAGGAGAAGAATAGTATACTCCATGGTGGACTGTGGTTCTACAACAATTCCGCGCTCATCCTTATGGACTATGACGACATAAGTGATCCAAGCGGGTGTTCATTGGATCTGTTGGAGGTTTGGATCAAAGTACGGGGACTTCGAGTTGCCATGCGCAAGGAAAAGGTCCTCGCTCAACTTGGGAATGTGCTGGGTTCATTTATTCGGGTCTATCCGGCGACTTTGATGCGGAAAGAATTATGCACGATGTTGTATCACCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 zf-CCHC_4

Protein Analysis

232

Amino Acids

25.33

Weight (kDa)

8.53

Isoelectric Point (pI)

32.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000123)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G38140
fragaria_vesca FvH4_1g20041 FvH4_1g21262 FvH4_1g25072 FvH4_2g16250 FvH4_2g17190 FvH4_3g04330 FvH4_3g20250 FvH4_3g22272 FvH4_3g26421 FvH4_3g35981 FvH4_4g01621 FvH4_4g07081 FvH4_4g13902 FvH4_4g18461 FvH4_5g15851 FvH4_6g06531 FvH4_6g22500 FvH4_6g23161 FvH4_6g26861 FvH4_6g28682 FvH4_6g33163 FvH4_6g33591 FvH4_6g38140 FvH4_7g00272
malus_domestica MD05G1145500.v1.1 MD10G1144900.v1.1
prunus_persica Prupe.8G183300_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0109311 RchiOBHm_Chr2g0109451 RchiOBHm_Chr2g0164071 RchiOBHm_Chr3g0469891 RchiOBHm_Chr3g0477271 RchiOBHm_Chr6g0281681
rosa_laevigata RLG00000007350 RLG00000011905 RLG00000012951 RLG00000030154
rosa_multiflora Rmu_co8029630.1_g000001 Rmu_co8038194.1_g000001 Rmu_co8114330.1_g000001 Rmu_co8138832.1_g000001 Rmu_co8166524.1_g000001 Rmu_co8169592.1_g000001 Rmu_co8214120.1_g000001 Rmu_co8224140.1_g000001 Rmu_co8242461.1_g000001 Rmu_co8314423.1_g000002 Rmu_co8333117.1_g000001 Rmu_co8348539.1_g000002 Rmu_co8348927.1_g000001 Rmu_co8416111.1_g000001 Rmu_co8444475.1_g000001 Rmu_co8503525.1_g000001 Rmu_sc0000047.1_g000017 Rmu_sc0000076.1_g000047 Rmu_sc0000116.1_g000002 Rmu_sc0000146.1_g000004 Rmu_sc0000239.1_g000065 Rmu_sc0000335.1_g000105 Rmu_sc0000424.1_g000035 Rmu_sc0000517.1_g000021 Rmu_sc0000611.1_g000003 Rmu_sc0000770.1_g000050 Rmu_sc0000808.1_g000032 Rmu_sc0000811.1_g000026 Rmu_sc0000894.1_g000054 Rmu_sc0000898.1_g000082 Rmu_sc0000898.1_g000083 Rmu_sc0001350.1_g000029 Rmu_sc0001374.1_g000062 Rmu_sc0001405.1_g000018 Rmu_sc0001474.1_g000014 Rmu_sc0001526.1_g000021 Rmu_sc0001551.1_g000029 Rmu_sc0001557.1_g000004 Rmu_sc0001650.1_g000005 Rmu_sc0001650.1_g000013 Rmu_sc0001662.1_g000001 Rmu_sc0001673.1_g000030 Rmu_sc0001701.1_g000021 Rmu_sc0001814.1_g000019 Rmu_sc0002038.1_g000007 Rmu_sc0002179.1_g000011 Rmu_sc0002285.1_g000020 Rmu_sc0002575.1_g000023 Rmu_sc0002731.1_g000001 Rmu_sc0002944.1_g000021 Rmu_sc0003023.1_g000002 Rmu_sc0003129.1_g000006 Rmu_sc0003144.1_g000013 Rmu_sc0003156.1_g000010 Rmu_sc0003369.1_g000015 Rmu_sc0003410.1_g000028 Rmu_sc0003410.1_g000029 Rmu_sc0003507.1_g000033 Rmu_sc0003548.1_g000013 Rmu_sc0003605.1_g000027 Rmu_sc0003710.1_g000010 Rmu_sc0003863.1_g000009 Rmu_sc0003909.1_g000006 Rmu_sc0003916.1_g000023 Rmu_sc0003916.1_g000024 Rmu_sc0003930.1_g000009 Rmu_sc0004030.1_g000006 Rmu_sc0004046.1_g000018 Rmu_sc0004154.1_g000010 Rmu_sc0004191.1_g000011 Rmu_sc0004205.1_g000007 Rmu_sc0004556.1_g000006 Rmu_sc0004975.1_g000008 Rmu_sc0005038.1_g000028 Rmu_sc0005083.1_g000005 Rmu_sc0005220.1_g000016 Rmu_sc0005274.1_g000011 Rmu_sc0005378.1_g000023 Rmu_sc0005385.1_g000016 Rmu_sc0005424.1_g000023 Rmu_sc0005738.1_g000009 Rmu_sc0005798.1_g000015 Rmu_sc0005834.1_g000008 Rmu_sc0006128.1_g000007 Rmu_sc0006865.1_g000015 Rmu_sc0006925.1_g000001 Rmu_sc0007005.1_g000002 Rmu_sc0007025.1_g000017 Rmu_sc0007518.1_g000004 Rmu_sc0007813.1_g000002 Rmu_sc0008730.1_g000003 Rmu_sc0008861.1_g000008 Rmu_sc0008861.1_g000009 Rmu_sc0009624.1_g000009 Rmu_sc0010439.1_g000001 Rmu_sc0010719.1_g000003 Rmu_sc0011069.1_g000001 Rmu_sc0013102.1_g000007 Rmu_sc0013803.1_g000022 Rmu_sc0013860.1_g000004 Rmu_sc0014885.1_g000001 Rmu_sc0017338.1_g000001 Rmu_sc0017964.1_g000006 Rmu_sc0019100.1_g000002 Rmu_sc0020554.1_g000001 Rmu_sc0020928.1_g000001 Rmu_sc0021886.1_g000001 Rmu_sc0022276.1_g000001 Rmu_sc0022942.1_g000001 Rmu_sc0022945.1_g000001 Rmu_sc0023180.1_g000002 Rmu_sc0024149.1_g000001 Rmu_sc0024149.1_g000002 Rmu_sc0026250.1_g000001 Rmu_sc0029882.1_g000003 Rmu_sc0035589.1_g000001 Rmu_sc0035888.1_g000003 Rmu_sc0036257.1_g000001 Rmu_sc0036258.1_g000001 Rmu_sc0040283.1_g000002 Rmu_sc0042169.1_g000001 Rmu_ssc0000126.1_g000076 Rmu_ssc0000357.1_g000017 Rmu_ssc0000372.1_g000063 Rmu_ssc0000391.1_g000030 Rmu_ssc0000432.1_g000006 Rmu_ssc0000480.1_g000008
rosa_roxburghii Rroxscaffold_1G00047150 Rroxscaffold_2G00124620 Rroxscaffold_2G00130500 Rroxscaffold_2G00146610 Rroxscaffold_3G00260760 Rroxscaffold_3G00270900 Rroxscaffold_4G00282170 Rroxscaffold_6G00418750 Rroxscaffold_7G00186930
rosa_rugosa Rorug01G0267800 Rorug02G0132500 Rorug02G0156000 Rorug02G0163300 Rorug02G0282900 Rorug02G0283000 Rorug02G0439400 Rorug02G0488800 Rorug02G0488900 Rorug02G0556100 Rorug03G0101100 Rorug03G0101200 Rorug03G0130200 Rorug03G0223700 Rorug04G0015200 Rorug04G0032400 Rorug04G0043600 Rorug04G0049700 Rorug04G0137000 Rorug04G0178300 Rorug04G0198500 Rorug05G0298400 Rorug05G0438000 Rorug05G0499300 Rorug06G0025400 Rorug06G0142400 Rorug07G0108200 Rorug07G0111200
rosa_samantha Rh1CG412100 Rh2AG574700 Rh2BG495100 Rh2CG255400 Rh2CG263700 Rh2CG548300 Rh3BG288300 Rh3DG068500 Rh5CG272200 Rh5DG249700 Rh5DG397400 Rh6AG255100 Rh6BG258500 Rh6CG257300 Rh6DG249500 Rh6DG268300 Rh6DG381800 Rh7BG282800 Rh7BG372100
rosa_wichuraiana Rw4G024160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 587
AccI GTMKAC 2 cut(s) 65, 448
AccII CGCG 1 cut(s) 485
AciI CCGC 6 cut(s) 217, 270, 352, 483, 525, 661
AclWI GGATC 5 cut(s) 163, 190, 512, 546, 563
AfaI GTAC 1 cut(s) 564
AfeI AGCGCT 1 cut(s) 247
AfiI CCNNNNNNNGG 3 cut(s) 68, 129, 186
AgsI TTSAA 2 cut(s) 223, 424
AjnI CCWGG 1 cut(s) 355
AjuI GAANNNNNNNTTGG 4 cut(s) 96, 128, 514, 546
AleI CACNNNNGTG 1 cut(s) 95
AluBI AGCT 1 cut(s) 259
AluI AGCT 1 cut(s) 259
Alw26I GTCTC 2 cut(s) 70, 300
AlwI GGATC 5 cut(s) 163, 190, 512, 546, 563
Ama87I CYCGRG 1 cut(s) 52
Aor51HI AGCGCT 1 cut(s) 247
AoxI GGCC 2 cut(s) 115, 211
ApeKI GCWGC 2 cut(s) 243, 329
AspLEI GCGC 3 cut(s) 248, 487, 588
AspS9I GGNCC 2 cut(s) 7, 598
AsuHPI GGTGA 2 cut(s) 386, 680
AvaI CYCGRG 1 cut(s) 52
AvaII GGWCC 2 cut(s) 7, 598
BarI GAAGNNNNNNTAC 2 cut(s) 555, 587
BbvCI CCTCAGC 1 cut(s) 231
BbvI GCAGC 2 cut(s) 255, 341
BccI CCATC 1 cut(s) 377
BceAI ACGGC 2 cut(s) 46, 256
BcgI CGANNNNNNTGC 2 cut(s) 640, 674
BciT130I CCWGG 1 cut(s) 357
BcoDI GTCTC 2 cut(s) 70, 300
BfaI CTAG 3 cut(s) 44, 282, 293
BfmI CTRYAG 1 cut(s) 159
BfoI RGCGCY 1 cut(s) 249
BglI GCCNNNNNGGC 1 cut(s) 360
BglII AGATCT 1 cut(s) 295
BisI GCNGC 2 cut(s) 244, 330
BlsI GCNGC 2 cut(s) 245, 331
Bme1390I CCNGG 1 cut(s) 357
Bme18I GGWCC 2 cut(s) 7, 598
BmeT110I CYCGRG 1 cut(s) 52
BmgT120I GGNCC 2 cut(s) 7, 598
BmrFI CCNGG 1 cut(s) 357
BmsI GCATC 1 cut(s) 648
Bpu10I CCTNAGC 1 cut(s) 231
BsaJI CCNNGG 2 cut(s) 355, 454
Bsc4I CCNNNNNNNGG 3 cut(s) 68, 129, 186
Bse118I RCCGGY 3 cut(s) 47, 213, 332
Bse1I ACTGG 2 cut(s) 123, 314
BseBI CCWGG 1 cut(s) 357
BseDI CCNNGG 2 cut(s) 355, 454
BseGI GGATG 3 cut(s) 300, 388, 489
BseLI CCNNNNNNNGG 3 cut(s) 68, 129, 186
BseMII CTCAG 1 cut(s) 222
BseNI ACTGG 2 cut(s) 123, 314
BseRI GAGGAG 2 cut(s) 410, 449
BseXI GCAGC 2 cut(s) 255, 341
BseYI CCCAGC 1 cut(s) 622
BsgI GTGCAG 2 cut(s) 73, 348
Bsh1236I CGCG 1 cut(s) 485
BshFI GGCC 2 cut(s) 117, 213
BsiHKCI CYCGRG 1 cut(s) 52
BsiSI CCGG 5 cut(s) 48, 180, 214, 333, 647
BslFI GGGAC 2 cut(s) 303, 582
BslI CCNNNNNNNGG 3 cut(s) 68, 129, 186
BsmAI GTCTC 2 cut(s) 70, 300
BsmFI GGGAC 2 cut(s) 303, 582
BsnI GGCC 2 cut(s) 117, 213
BsoBI CYCGRG 1 cut(s) 52
Bsp143I GATC 6 cut(s) 155, 182, 295, 517, 538, 555
Bsp19I CCATGG 1 cut(s) 454
BspACI CCGC 6 cut(s) 217, 270, 352, 483, 525, 661
BspANI GGCC 2 cut(s) 117, 213
BspCNI CTCAG 1 cut(s) 223
BspFNI CGCG 1 cut(s) 485
BspMAI CTGCAG 1 cut(s) 163
BspPI GGATC 5 cut(s) 163, 190, 512, 546, 563
BsrFI RCCGGY 3 cut(s) 47, 213, 332
BsrI ACTGG 2 cut(s) 123, 314
BssAI RCCGGY 3 cut(s) 47, 213, 332
BssECI CCNNGG 2 cut(s) 355, 454
BssMI GATC 6 cut(s) 155, 182, 295, 517, 538, 555
BssNAI GTATAC 1 cut(s) 449
BssT1I CCWWGG 1 cut(s) 454
Bst1107I GTATAC 1 cut(s) 449
Bst2UI CCWGG 1 cut(s) 357
Bst4CI ACNGT 2 cut(s) 81, 465
BstC8I GCNNGC 3 cut(s) 49, 215, 334
BstDEI CTNAG 1 cut(s) 231
BstDSI CCRYGG 1 cut(s) 454
BstF5I GGATG 3 cut(s) 300, 388, 489
BstFNI CGCG 1 cut(s) 485
BstH2I RGCGCY 1 cut(s) 249
BstHHI GCGC 3 cut(s) 248, 487, 588
BstKTI GATC 6 cut(s) 158, 185, 298, 520, 541, 558
BstMAI GTCTC 2 cut(s) 70, 300
BstMBI GATC 6 cut(s) 155, 182, 295, 517, 538, 555
BstMWI GCNNNNNNNGC 2 cut(s) 196, 360
BstNI CCWGG 1 cut(s) 357
BstSCI CCNGG 1 cut(s) 355
BstSFI CTRYAG 1 cut(s) 159
BstUI CGCG 1 cut(s) 485
BstV1I GCAGC 2 cut(s) 255, 341
BstX2I RGATCY 3 cut(s) 155, 295, 538
BstXI CCANNNNNNTGG 1 cut(s) 100
BstYI RGATCY 3 cut(s) 155, 295, 538
BstZ17I GTATAC 1 cut(s) 449
BsuRI GGCC 2 cut(s) 117, 213
BtgI CCRYGG 1 cut(s) 454
BtsCI GGATG 3 cut(s) 300, 388, 489
BtsIMutI CAGTG 1 cut(s) 130
Cac8I GCNNGC 3 cut(s) 49, 215, 334
CfoI GCGC 3 cut(s) 248, 487, 588
Cfr10I RCCGGY 3 cut(s) 47, 213, 332
Cfr13I GGNCC 2 cut(s) 7, 598
Csp6I GTAC 1 cut(s) 563
CviAII CATG 2 cut(s) 455, 583
CviQI GTAC 1 cut(s) 563
DdeI CTNAG 1 cut(s) 231
DpnI GATC 6 cut(s) 157, 184, 297, 519, 540, 557
DpnII GATC 6 cut(s) 155, 182, 295, 517, 538, 555
EciI GGCGGA 1 cut(s) 341
Eco130I CCWWGG 1 cut(s) 454
Eco47I GGWCC 2 cut(s) 7, 598
Eco47III AGCGCT 1 cut(s) 247
Eco88I CYCGRG 1 cut(s) 52
EcoO109I RGGNCCY 1 cut(s) 598
EcoRII CCWGG 1 cut(s) 355
EcoT14I CCWWGG 1 cut(s) 454
ErhI CCWWGG 1 cut(s) 454
FaeI CATG 2 cut(s) 458, 586
FaqI GGGAC 2 cut(s) 303, 582
FatI CATG 2 cut(s) 454, 582
FauI CCCGC 1 cut(s) 518
FblI GTMKAC 2 cut(s) 65, 448
Fnu4HI GCNGC 2 cut(s) 244, 330
FokI GGATG 3 cut(s) 287, 395, 476
Fsp4HI GCNGC 2 cut(s) 244, 330
FspBI CTAG 3 cut(s) 44, 282, 293
FspI TGCGCA 1 cut(s) 587
GlaI GCGC 3 cut(s) 247, 486, 587
GluI GCNGC 2 cut(s) 244, 330
GsaI CCCAGC 1 cut(s) 626
HaeII RGCGCY 1 cut(s) 249
HaeIII GGCC 2 cut(s) 117, 213
HapII CCGG 5 cut(s) 48, 180, 214, 333, 647
HhaI GCGC 3 cut(s) 248, 487, 588
Hin1II CATG 2 cut(s) 458, 586
Hin6I GCGC 3 cut(s) 246, 485, 586
HinP1I GCGC 3 cut(s) 246, 485, 586
HincII GTYRAC 1 cut(s) 66
HindII GTYRAC 1 cut(s) 66
HinfI GANTC 1 cut(s) 405
HpaII CCGG 5 cut(s) 48, 180, 214, 333, 647
HphI GGTGA 2 cut(s) 386, 680
Hpy166II GTNNAC 3 cut(s) 66, 449, 461
Hpy188I TCNGA 4 cut(s) 85, 263, 305, 404
Hpy188III TCNNGA 3 cut(s) 54, 282, 392
Hpy8I GTNNAC 3 cut(s) 66, 449, 461
Hpy99I CGWCG 1 cut(s) 67
HpyAV CCTTC 2 cut(s) 122, 217
HpyCH4III ACNGT 2 cut(s) 81, 465
HpyCH4V TGCA 8 cut(s) 4, 21, 36, 90, 161, 171, 329, 675
HpyF10VI GCNNNNNNNGC 2 cut(s) 196, 360
HpyF3I CTNAG 1 cut(s) 231
Hsp92II CATG 2 cut(s) 458, 586
HspAI GCGC 3 cut(s) 246, 485, 586
KroI GCCGGC 3 cut(s) 47, 213, 332
KroNI GCCGGC 3 cut(s) 49, 215, 334
Kzo9I GATC 6 cut(s) 155, 182, 295, 517, 538, 555
Lsp1109I GCAGC 2 cut(s) 255, 341
LweI GCATC 1 cut(s) 648
MaeI CTAG 3 cut(s) 44, 282, 293
MaeIII GTNAC 1 cut(s) 275
MalI GATC 6 cut(s) 157, 184, 297, 519, 540, 557
MboI GATC 6 cut(s) 155, 182, 295, 517, 538, 555
MboII GAAGA 1 cut(s) 451
MflI RGATCY 3 cut(s) 155, 295, 538
MluCI AATT 2 cut(s) 478, 668
MmeI TCCRAC 4 cut(s) 81, 108, 382, 525
MroNI GCCGGC 3 cut(s) 47, 213, 332
MslI CAYNNNNRTG 2 cut(s) 95, 494
MspI CCGG 5 cut(s) 48, 180, 214, 333, 647
MspR9I CCNGG 1 cut(s) 357
MvaI CCWGG 1 cut(s) 357
MvnI CGCG 1 cut(s) 485
MwoI GCNNNNNNNGC 2 cut(s) 196, 360
NaeI GCCGGC 3 cut(s) 49, 215, 334
NcoI CCATGG 1 cut(s) 454
NdeII GATC 6 cut(s) 155, 182, 295, 517, 538, 555
NgoMIV GCCGGC 3 cut(s) 47, 213, 332
NlaIII CATG 2 cut(s) 458, 586
NsbI TGCGCA 1 cut(s) 587
OliI CACNNNNGTG 1 cut(s) 95
PaeR7I CTCGAG 1 cut(s) 52
PcsI WCGNNNNNNNCGW 2 cut(s) 59, 571
PdiI GCCGGC 3 cut(s) 49, 215, 334
PfeI GAWTC 1 cut(s) 405
PkrI GCNGC 2 cut(s) 245, 331
PpuMI RGGWCCY 1 cut(s) 598
Psp5II RGGWCCY 1 cut(s) 598
Psp6I CCWGG 1 cut(s) 355
PspFI CCCAGC 1 cut(s) 622
PspGI CCWGG 1 cut(s) 355
PspPI GGNCC 2 cut(s) 7, 598
PspPPI RGGWCCY 1 cut(s) 598
PstI CTGCAG 1 cut(s) 163
PsuI RGATCY 3 cut(s) 155, 295, 538
RsaI GTAC 1 cut(s) 564
RsaNI GTAC 1 cut(s) 563
RseI CAYNNNNRTG 2 cut(s) 95, 494
SalI GTCGAC 1 cut(s) 64
SatI GCNGC 2 cut(s) 244, 330
Sau3AI GATC 6 cut(s) 155, 182, 295, 517, 538, 555
Sau96I GGNCC 2 cut(s) 7, 598
ScrFI CCNGG 1 cut(s) 357
SfaNI GCATC 1 cut(s) 648
SfcI CTRYAG 1 cut(s) 159
Sfr274I CTCGAG 1 cut(s) 52
SinI GGWCC 2 cut(s) 7, 598
SlaI CTCGAG 1 cut(s) 52
SmiMI CAYNNNNRTG 2 cut(s) 95, 494
SmlI CTYRAG 1 cut(s) 52
SmoI CTYRAG 1 cut(s) 52
Sse9I AATT 2 cut(s) 478, 668
SsiI CCGC 6 cut(s) 217, 270, 352, 483, 525, 661
SspMI CTAG 3 cut(s) 44, 282, 293
StyD4I CCNGG 1 cut(s) 355
StyI CCWWGG 1 cut(s) 454
TaaI ACNGT 2 cut(s) 81, 465
TaqI TCGA 3 cut(s) 53, 65, 574
TasI AATT 2 cut(s) 478, 668
TfiI GAWTC 1 cut(s) 405
TscAI CASTG 1 cut(s) 130
TseI GCWGC 2 cut(s) 243, 329
TspDTI ATGAA 2 cut(s) 522, 618
TspRI CASTG 1 cut(s) 130
VpaK11BI GGWCC 2 cut(s) 7, 598
XbaI TCTAGA 1 cut(s) 281
XhoI CTCGAG 1 cut(s) 52
XmiI GTMKAC 2 cut(s) 65, 448
XspI CTAG 3 cut(s) 44, 282, 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.