Rorug04G0015200

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000123
Physical Location & Seq
Forward (+)
2390969 .. 2394906
3938 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0015200.1

Sequence Viewer

Length: 3348 bp
ATGATCTTTTACAAGGAGAAAACATATATACGGAAGGGAAGGGAAAAGGAAGACAACAAAAGACTCATAATTATTTCCAATATCAAGATACTTGACTGCAGAAAATCCAAAGGACAGAAGTTTAGAGCTGTTTTCTACAAGGTGGCTCCCTCAGATGTACGACACCAAACAGGTGCTTTCGGTGACGCATTTGCTAAAATTGATCAATGCAAATACAAGGATAGCATCGCCAAATGGAGGAAGGCTCTTGAGGAAGCAGCAAATTTGTCTGGATGGACTTACGAGGAAGACCGGTCCGAAGCTGAATTTATCAAGAAGATTGTTGGGGACTTGTCCGCCCGAGTAGTAAACCCTTCATATGACTTGGATGTTGCTAAACATCCAATTGGATTAGAGTCTTGTAGACAAGAGGTCAATAGTCTTTTAAAAGTCGAGGAAAATATTGTTCGCATGGTGGGCATATGGGGGACTGGTGGAATAGGAAAAACTACAATTGTGAAAGCTGTGTTTAATTCAATTCGCCATAAGTTTGAATGTTGCTGTTTCTTGACAGATGTTAGATCAAAAGACCTAGCCCAACTGCAAGAGACACTTTTGTCTGATATTTTAAACTCAACTTTGAAGGTGAGCAGTGTTGATCAAGGAGTCAGTTTTATAAAGACAATGATGCGACATAAAAAAGTTCTCTTAATCCTTGATGACGTGAGTGATTCTAGCCAATTAGATAAATTAGTTCCATTCCCTAATTGTTTTGGGCTGGGCAGTAGAATTCTCATAACAACAAGAGATAAACGTTGTCTAACAGCTTATCCAGTTAATGAAGTATACGAGGTCAAAATTTTAGATGACGGTCAAGCTTTGAAGTTGTTTAGCCTGAATGCATTCAAAAGTAATGGACCTCCACATGCTTACCTGGAACTTGCACGACATGCAGTACGCTATGCCCAAGGCCTTCCATTAACTTTGATAGTTTTAGGCTCCCATCTATTTAATAGAAGCATGGAGGAGTGGAAAGCTACAATAGGTAGTCGTAAAGGAGGTCCTCAGGCAGATATACAGAAAGTTCTCAAACTAAGTTATGATGCTCTAAAAAAGGATTTGCAAGAACTATTTCTTGATATTGCTTGCTTCTTTAAAGGTATGCATGCAGCCCATGTGAAACCAATACTAGAAGCTTGCTACGACCACAAAACAATGGTGATTGGTATTGCACAATTGCAAGAAAAAGCCTTGGTAAGAATTGACGAAGAACATATTTGGATGCATGACTTGATAGAAGAAATGGGTAAAGACATAGTGTTTCAAGAGTCACCTGATGAACCCGGGGAACGCAGCAGAGTGTGGAGTGCAGAAGATGTCAACGACGTTCTAACAAATAATAAAGGAACAAATAAGGTTGTAGGCATTCAGGCCCCATGGGAATTATCTTCGATCTCTTTGAATGCTAAAAGCTTCTTAGAGATGAAGAAGCTTAGATATATTTCTATTGGCAACATAATGATGAGATCTGAATCATTCTCTGGAGATATTGATTATCTCTCCAACCAGTTGAGGTGGCTTGATTGGCCAGAATGTCCGTTGCAATCTTTTCCATCTGATTTTCATGCAAACAGACTTGTAAATCTCAATATTTATGGGAGTCGCAGAATCACACGACTATGGGAGGGGCATAAGAATTTTTTAAGGCTAACATGTATGGATTTAGGCGATTGTACATCCCTAAAGGAACTCCCAGATTTGAGTGGAATCCCTAACTTGAAAGAGTTGGATCTATCTAACTGCACCGGTTTAGTTGAGGTTCCTGGTTCAATTCGATTCCTTCATAACCTTGTTACCTTGAATTTAAGGGGTTGGGTATCCCTAAAGGAACTCCCAGACTTGAGTGGAATCCCTAACTTGAAAGAGTTGAATTTAAGTCGTTGTACATCCCTAAAGGAACTCCCAGACTTGAGTGGAATCCCTAACTTGAAAGAGTTGGATCTATCTGAGTGTACCAGTTTAGTTAAGATTCCTTCAATTCGATTCCTTCATAACCTTGTTACCTTGAATTTAAGTAGTTGTGTATCCTTAAAGGAACTCTCAGACTTGAGTGGAATCCCTAATTTGAAAAAGTTGAATGTATATAAGTGTATCGATTTAGTTGAGGTTCCTGATTCAATTCGATTCCTTCATAACCTTGTTACCTTGAATTTAAGGGGTTGTAAATCCTTAAAGGAACTCCCAGACTTGAGTGGAATCCCTAACTTGAAAGAGTTGGATCTATCTGAGTGTACCAGTTTAGTTAAGATTTCTGATTCTGTTAGATTGCTTGATAAGCTTGTTACCTTGAATGTTAAAGGTTGCTCTAACCTTATTATGTTTCCAAGAAAAATCAACTGGAAATCTGTAGAAACAATCTCTACGATGTTTTGCAAGCTTGAGGAATTTTCAGAAGTTGGGGAAGAGATGGTTTCCTTGAGAAAATTGAATCTAGCAGCCACTCGCATTAAAGAATTGCATCCGTCCATTACAAAACTTATTGGGCTAGAAGAGTTGATTCTAGGCGCTTGTGAAAATCTTACAACTCTACCGTATAATATTTATGAGTTGCACAATCTAAAGATTCTTTTTGCGACGGGATGCTTTAAACTGGCTACATTTCCAGAAATTCCAATAAAGATGGATTCCTTGAGAGATCTCCATCTTCCAGGCAGTGGCATAAGAGAATTGGATGAGTCAATTAAAAATCTCATTGGGCTTCAAAGGTTGGATCTTCGTTGTTGCAAAAATCTCACAACTATACCGTGCAACATTTATGGGTTGCAAAATTTGGTGGGTCTTGATCTTAGTGGCTGCTCAAAACTTGTCAGATTTCCAACAAATACCAAAATTTTGAATGTCGATGGTTGCTCGCTATCACTTCCCAAGCTACGGCAGTTGGATATCAGCGGATGCACTAGTTTATCAGATTGTGATTTCCTGATGACTCTTGATTGCTGGGAAACATTAGAGTCGCTTGATCTGTCAGGATGCAATTTTGTTAGTCTTCCTGCTTGCCTCACCAAATTTGTCAACTTGAAGAAGATTCGTGGAGCTGTGATAGAGGCGGCGTCGCCGTCAATTGAAGAGCCAACTCCGTCATCATGGCTTTCTTTGTCTTCCGAAACTCGGAAACGACACCGCAGCGCGGAAGAACCATCACCAACACCAGTGAATCCTCCGAAACGACAACGCACTGCCGCCGGGGGGCAAGCACCGGTGGTCCCCATGTTCCCTGACCATCACTCAACTACTAGTCCACAGCTTCAACACCAAGACGAATCAACTGAGCTGTTAAGGCTTTCTCTGTTAAGGCTGTCTCTGTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00670 ko01100 ko01110 ko01130 ko01523 map00230 map00670 map01100 map01110 map01130 map01523
Pfam Domains
Protein Families
DDE_Tnp_1_7 DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

1115

Amino Acids

125.23

Weight (kDa)

8.02

Isoelectric Point (pI)

45.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 29 - 114 6.1e-18 TIR domain
NB-ARC PF00931 139 - 297 4e-23 NB-ARC domain
WHD_ROQ1 PF23282 368 - 436 2.1e-13 Disease resistance protein Roq1-like, winged-helix domain
LRR_4 PF12799 631 - 671 8.8e-06 Leucine Rich repeats (2 copies)
LRR_13 PF23286 865 - 1017 1.9e-17 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 889 - 1002 8e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000123)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G38140
fragaria_vesca FvH4_1g20041 FvH4_1g21262 FvH4_1g25072 FvH4_2g16250 FvH4_2g17190 FvH4_3g04330 FvH4_3g20250 FvH4_3g22272 FvH4_3g26421 FvH4_3g35981 FvH4_4g01621 FvH4_4g07081 FvH4_4g13902 FvH4_4g18461 FvH4_5g15851 FvH4_6g06531 FvH4_6g22500 FvH4_6g23161 FvH4_6g26861 FvH4_6g28682 FvH4_6g33163 FvH4_6g33591 FvH4_6g38140 FvH4_7g00272
malus_domestica MD05G1145500.v1.1 MD10G1144900.v1.1
prunus_persica Prupe.8G183300_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0109311 RchiOBHm_Chr2g0109451 RchiOBHm_Chr2g0164071 RchiOBHm_Chr3g0469891 RchiOBHm_Chr3g0477271 RchiOBHm_Chr6g0281681
rosa_laevigata RLG00000007350 RLG00000011905 RLG00000012951 RLG00000030154
rosa_multiflora Rmu_co8029630.1_g000001 Rmu_co8038194.1_g000001 Rmu_co8114330.1_g000001 Rmu_co8138832.1_g000001 Rmu_co8166524.1_g000001 Rmu_co8169592.1_g000001 Rmu_co8214120.1_g000001 Rmu_co8224140.1_g000001 Rmu_co8242461.1_g000001 Rmu_co8314423.1_g000002 Rmu_co8333117.1_g000001 Rmu_co8348539.1_g000002 Rmu_co8348927.1_g000001 Rmu_co8416111.1_g000001 Rmu_co8444475.1_g000001 Rmu_co8503525.1_g000001 Rmu_sc0000047.1_g000017 Rmu_sc0000076.1_g000047 Rmu_sc0000116.1_g000002 Rmu_sc0000146.1_g000004 Rmu_sc0000239.1_g000065 Rmu_sc0000335.1_g000105 Rmu_sc0000424.1_g000035 Rmu_sc0000517.1_g000021 Rmu_sc0000611.1_g000003 Rmu_sc0000770.1_g000050 Rmu_sc0000808.1_g000032 Rmu_sc0000811.1_g000026 Rmu_sc0000894.1_g000054 Rmu_sc0000898.1_g000082 Rmu_sc0000898.1_g000083 Rmu_sc0001350.1_g000029 Rmu_sc0001374.1_g000062 Rmu_sc0001405.1_g000018 Rmu_sc0001474.1_g000014 Rmu_sc0001526.1_g000021 Rmu_sc0001551.1_g000029 Rmu_sc0001557.1_g000004 Rmu_sc0001650.1_g000005 Rmu_sc0001650.1_g000013 Rmu_sc0001662.1_g000001 Rmu_sc0001673.1_g000030 Rmu_sc0001701.1_g000021 Rmu_sc0001814.1_g000019 Rmu_sc0002038.1_g000007 Rmu_sc0002179.1_g000011 Rmu_sc0002285.1_g000020 Rmu_sc0002575.1_g000023 Rmu_sc0002731.1_g000001 Rmu_sc0002944.1_g000021 Rmu_sc0003023.1_g000002 Rmu_sc0003129.1_g000006 Rmu_sc0003144.1_g000013 Rmu_sc0003156.1_g000010 Rmu_sc0003369.1_g000015 Rmu_sc0003410.1_g000028 Rmu_sc0003410.1_g000029 Rmu_sc0003507.1_g000033 Rmu_sc0003548.1_g000013 Rmu_sc0003605.1_g000027 Rmu_sc0003710.1_g000010 Rmu_sc0003863.1_g000009 Rmu_sc0003909.1_g000006 Rmu_sc0003916.1_g000023 Rmu_sc0003916.1_g000024 Rmu_sc0003930.1_g000009 Rmu_sc0004030.1_g000006 Rmu_sc0004046.1_g000018 Rmu_sc0004154.1_g000010 Rmu_sc0004191.1_g000011 Rmu_sc0004205.1_g000007 Rmu_sc0004556.1_g000006 Rmu_sc0004975.1_g000008 Rmu_sc0005038.1_g000028 Rmu_sc0005083.1_g000005 Rmu_sc0005220.1_g000016 Rmu_sc0005274.1_g000011 Rmu_sc0005378.1_g000023 Rmu_sc0005385.1_g000016 Rmu_sc0005424.1_g000023 Rmu_sc0005738.1_g000009 Rmu_sc0005798.1_g000015 Rmu_sc0005834.1_g000008 Rmu_sc0006128.1_g000007 Rmu_sc0006865.1_g000015 Rmu_sc0006925.1_g000001 Rmu_sc0007005.1_g000002 Rmu_sc0007025.1_g000017 Rmu_sc0007518.1_g000004 Rmu_sc0007813.1_g000002 Rmu_sc0008730.1_g000003 Rmu_sc0008861.1_g000008 Rmu_sc0008861.1_g000009 Rmu_sc0009624.1_g000009 Rmu_sc0010439.1_g000001 Rmu_sc0010719.1_g000003 Rmu_sc0011069.1_g000001 Rmu_sc0013102.1_g000007 Rmu_sc0013803.1_g000022 Rmu_sc0013860.1_g000004 Rmu_sc0014885.1_g000001 Rmu_sc0017338.1_g000001 Rmu_sc0017964.1_g000006 Rmu_sc0019100.1_g000002 Rmu_sc0020554.1_g000001 Rmu_sc0020928.1_g000001 Rmu_sc0021886.1_g000001 Rmu_sc0022276.1_g000001 Rmu_sc0022942.1_g000001 Rmu_sc0022945.1_g000001 Rmu_sc0023180.1_g000002 Rmu_sc0024149.1_g000001 Rmu_sc0024149.1_g000002 Rmu_sc0026250.1_g000001 Rmu_sc0029882.1_g000003 Rmu_sc0035589.1_g000001 Rmu_sc0035888.1_g000003 Rmu_sc0036257.1_g000001 Rmu_sc0036258.1_g000001 Rmu_sc0040283.1_g000002 Rmu_sc0042169.1_g000001 Rmu_ssc0000126.1_g000076 Rmu_ssc0000357.1_g000017 Rmu_ssc0000372.1_g000063 Rmu_ssc0000391.1_g000030 Rmu_ssc0000432.1_g000006 Rmu_ssc0000480.1_g000008
rosa_roxburghii Rroxscaffold_1G00047150 Rroxscaffold_2G00124620 Rroxscaffold_2G00130500 Rroxscaffold_2G00146610 Rroxscaffold_3G00260760 Rroxscaffold_3G00270900 Rroxscaffold_4G00282170 Rroxscaffold_6G00418750 Rroxscaffold_7G00186930
rosa_rugosa Rorug01G0267800 Rorug02G0132500 Rorug02G0156000 Rorug02G0163300 Rorug02G0282900 Rorug02G0283000 Rorug02G0439400 Rorug02G0488800 Rorug02G0488900 Rorug02G0556100 Rorug03G0101100 Rorug03G0101200 Rorug03G0130200 Rorug03G0223700 Rorug04G0015200 Rorug04G0032400 Rorug04G0043600 Rorug04G0049700 Rorug04G0137000 Rorug04G0178300 Rorug04G0198500 Rorug05G0298400 Rorug05G0438000 Rorug05G0499300 Rorug06G0025400 Rorug06G0142400 Rorug07G0108200 Rorug07G0111200
rosa_samantha Rh1CG412100 Rh2AG574700 Rh2BG495100 Rh2CG255400 Rh2CG263700 Rh2CG548300 Rh3BG288300 Rh3DG068500 Rh5CG272200 Rh5DG249700 Rh5DG397400 Rh6AG255100 Rh6BG258500 Rh6CG257300 Rh6DG249500 Rh6DG268300 Rh6DG381800 Rh7BG282800 Rh7BG372100
rosa_wichuraiana Rw4G024160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 656
AasI GACNNNNNNGTC 1 cut(s) 595
AccB7I CCANNNNNTGG 1 cut(s) 2693
AccI GTMKAC 2 cut(s) 403, 825
AccII CGCG 1 cut(s) 3167
AciI CCGC 6 cut(s) 336, 2928, 3086, 3160, 3167, 3219
AclI AACGTT 1 cut(s) 793
AclWI GGATC 4 cut(s) 1776, 1986, 2265, 2757
AcoI YGGCCR 1 cut(s) 1565
AcyI GRCGYC 1 cut(s) 3089
AfaI GTAC 6 cut(s) 159, 936, 1714, 1924, 1993, 2272
AfiI CCNNNNNNNGG 6 cut(s) 237, 2693, 2910, 3147, 3166, 3225
AflIII ACRYGT 1 cut(s) 1691
AgeI ACCGGT 3 cut(s) 291, 1784, 3235
AhdI GACNNNNNGTC 1 cut(s) 410
AhlI ACTAGT 2 cut(s) 2936, 3272
AjiI CACGTC 1 cut(s) 703
AjnI CCWGG 3 cut(s) 912, 1801, 2686
Alw26I GTCTC 2 cut(s) 581, 3342
AlwI GGATC 4 cut(s) 1776, 1986, 2265, 2757
Ama87I CYCGRG 2 cut(s) 339, 1322
AoxI GGCC 3 cut(s) 949, 1410, 1565
ApeKI GCWGC 6 cut(s) 257, 1148, 1332, 2474, 2832, 3162
AsiGI ACCGGT 3 cut(s) 291, 1784, 3235
Asp700I GAANNNNTTC 2 cut(s) 881, 1110
AspLEI GCGC 2 cut(s) 2546, 3167
AspS9I GGNCC 5 cut(s) 294, 896, 1040, 1411, 3241
AsuC2I CCSGG 3 cut(s) 1323, 1324, 3223
AsuHPI GGTGA 6 cut(s) 194, 637, 1210, 1302, 3031, 3171
AvaI CYCGRG 2 cut(s) 339, 1322
AvaII GGWCC 4 cut(s) 294, 896, 1040, 3241
AxyI CCTNAGG 1 cut(s) 1044
BalI TGGCCA 1 cut(s) 1567
BbsI GAAGAC 4 cut(s) 57, 294, 3017, 3129
BbvI GCAGC 6 cut(s) 269, 1160, 1344, 2486, 2819, 3174
BccI CCATC 9 cut(s) 267, 990, 1600, 2440, 2653, 2688, 2876, 3184, 3267
BceAI ACGGC 2 cut(s) 2927, 3079
BciT130I CCWGG 3 cut(s) 914, 1803, 2688
BciVI GTATCC 2 cut(s) 1867, 2074
BclI TGATCA 2 cut(s) 202, 637
BcnI CCSGG 3 cut(s) 1323, 1324, 3223
BcoDI GTCTC 2 cut(s) 581, 3342
BcuI ACTAGT 2 cut(s) 2936, 3272
BfaI CTAG 8 cut(s) 572, 714, 1169, 2471, 2525, 2540, 2937, 3273
BfmI CTRYAG 2 cut(s) 97, 2385
BfoI RGCGCY 1 cut(s) 2547
BfuI GTATCC 2 cut(s) 1867, 2074
BglII AGATCT 2 cut(s) 1505, 2674
BisI GCNGC 8 cut(s) 258, 1149, 1333, 2475, 2833, 3087, 3163, 3219
BlsI GCNGC 8 cut(s) 259, 1150, 1334, 2476, 2834, 3088, 3164, 3220
Bme1390I CCNGG 6 cut(s) 914, 1323, 1324, 1803, 2688, 3223
Bme18I GGWCC 4 cut(s) 294, 896, 1040, 3241
BmeRI GACNNNNNGTC 1 cut(s) 410
BmeT110I CYCGRG 2 cut(s) 339, 1322
BmgBI CACGTC 1 cut(s) 703
BmgT120I GGNCC 5 cut(s) 294, 896, 1040, 1411, 3241
BmiI GGNNCC 6 cut(s) 147, 979, 1413, 1800, 2148, 3243
BmrFI CCNGG 6 cut(s) 914, 1323, 1324, 1803, 2688, 3223
BmsI GCATC 8 cut(s) 234, 657, 1072, 1251, 2506, 2609, 2921, 2999
BpiI GAAGAC 4 cut(s) 57, 294, 3017, 3129
BplI GAGNNNNNCTC 2 cut(s) 229, 261
BpmI CTGGAG 1 cut(s) 1542
BpuEI CTTGAG 8 cut(s) 269, 1900, 1969, 2107, 2248, 2438, 2476, 2689
BpuMI CCSGG 3 cut(s) 1323, 1324, 3223
Bsa29I ATCGAT 1 cut(s) 2133
BsaBI GATNNNNATC 2 cut(s) 1510, 2679
BsaHI GRCGYC 1 cut(s) 3089
BsaJI CCNNGG 6 cut(s) 946, 1230, 1322, 1323, 1415, 3222
BsaWI WCCGGW 3 cut(s) 291, 1784, 3235
Bsc4I CCNNNNNNNGG 6 cut(s) 237, 2693, 2910, 3147, 3166, 3225
Bse118I RCCGGY 3 cut(s) 291, 1784, 3235
Bse1I ACTGG 8 cut(s) 475, 812, 1546, 1995, 2274, 2381, 2634, 3188
Bse21I CCTNAGG 1 cut(s) 1044
Bse8I GATNNNNATC 2 cut(s) 1510, 2679
BseBI CCWGG 3 cut(s) 914, 1803, 2688
BseCI ATCGAT 1 cut(s) 2133
BseDI CCNNGG 6 cut(s) 946, 1230, 1322, 1323, 1415, 3222
BseJI GATNNNNATC 2 cut(s) 1510, 2679
BseLI CCNNNNNNNGG 6 cut(s) 237, 2693, 2910, 3147, 3166, 3225
BseMII CTCAG 6 cut(s) 165, 1058, 1977, 2094, 2256, 3297
BseNI ACTGG 8 cut(s) 475, 812, 1546, 1995, 2274, 2381, 2634, 3188
BseRI GAGGAG 1 cut(s) 1019
BseXI GCAGC 6 cut(s) 269, 1160, 1344, 2486, 2819, 3174
BseYI CCCAGC 2 cut(s) 757, 2976
BsgI GTGCAG 2 cut(s) 1368, 1765
Bsh1236I CGCG 1 cut(s) 3167
BshFI GGCC 3 cut(s) 951, 1412, 1567
BshTI ACCGGT 3 cut(s) 291, 1784, 3235
BshVI ATCGAT 1 cut(s) 2133
BsiHKCI CYCGRG 2 cut(s) 339, 1322
BsiSI CCGG 5 cut(s) 292, 1323, 1785, 3222, 3236
BslFI GGGAC 3 cut(s) 341, 481, 3227
BslI CCNNNNNNNGG 6 cut(s) 237, 2693, 2910, 3147, 3166, 3225
BsmAI GTCTC 2 cut(s) 581, 3342
BsmFI GGGAC 3 cut(s) 341, 481, 3227
BsmI GAATGC 4 cut(s) 881, 883, 1404, 1447
BsnI GGCC 3 cut(s) 951, 1412, 1567
BsoBI CYCGRG 2 cut(s) 339, 1322
Bsp1407I TGTACA 2 cut(s) 1712, 1922
Bsp19I CCATGG 1 cut(s) 1415
BspACI CCGC 6 cut(s) 336, 2928, 3086, 3160, 3167, 3219
BspANI GGCC 3 cut(s) 951, 1412, 1567
BspCNI CTCAG 6 cut(s) 164, 1057, 1978, 2093, 2257, 3298
BspDI ATCGAT 1 cut(s) 2133
BspFNI CGCG 1 cut(s) 3167
BspLI GGNNCC 6 cut(s) 147, 979, 1413, 1800, 2148, 3243
BspMAI CTGCAG 1 cut(s) 101
BspPI GGATC 4 cut(s) 1776, 1986, 2265, 2757
BspQI GCTCTTC 1 cut(s) 3099
BsrFI RCCGGY 3 cut(s) 291, 1784, 3235
BsrGI TGTACA 2 cut(s) 1712, 1922
BsrI ACTGG 8 cut(s) 475, 812, 1546, 1995, 2274, 2381, 2634, 3188
BssAI RCCGGY 3 cut(s) 291, 1784, 3235
BssECI CCNNGG 6 cut(s) 946, 1230, 1322, 1323, 1415, 3222
BssNAI GTATAC 1 cut(s) 826
BssNI GRCGYC 1 cut(s) 3089
BssT1I CCWWGG 3 cut(s) 946, 1230, 1415
Bst1107I GTATAC 1 cut(s) 826
Bst2UI CCWGG 3 cut(s) 914, 1803, 2688
Bst4CI ACNGT 3 cut(s) 851, 2571, 2784
Bst6I CTCTTC 3 cut(s) 2436, 2523, 3099
BstACI GRCGYC 1 cut(s) 3089
BstAPI GCANNNNNTGC 1 cut(s) 929
BstAUI TGTACA 2 cut(s) 1712, 1922
BstC8I GCNNGC 7 cut(s) 1126, 1146, 1177, 2414, 2891, 3034, 3231
BstDSI CCRYGG 1 cut(s) 1415
BstFNI CGCG 1 cut(s) 3167
BstH2I RGCGCY 1 cut(s) 2547
BstHHI GCGC 2 cut(s) 2546, 3167
BstMAI GTCTC 2 cut(s) 581, 3342
BstMWI GCNNNNNNNGC 5 cut(s) 456, 929, 1564, 2314, 3316
BstNI CCWGG 3 cut(s) 914, 1803, 2688
BstNSI RCATGY 4 cut(s) 908, 932, 1148, 1695
BstSCI CCNGG 6 cut(s) 912, 1321, 1322, 1801, 2686, 3221
BstSFI CTRYAG 2 cut(s) 97, 2385
BstUI CGCG 1 cut(s) 3167
BstV1I GCAGC 6 cut(s) 269, 1160, 1344, 2486, 2819, 3174
BstV2I GAAGAC 4 cut(s) 57, 294, 3017, 3129
BstX2I RGATCY 6 cut(s) 1505, 1768, 1978, 2257, 2674, 2749
BstYI RGATCY 6 cut(s) 1505, 1768, 1978, 2257, 2674, 2749
BstZ17I GTATAC 1 cut(s) 826
Bsu15I ATCGAT 1 cut(s) 2133
Bsu36I CCTNAGG 1 cut(s) 1044
BsuI GTATCC 2 cut(s) 1867, 2074
BsuRI GGCC 3 cut(s) 951, 1412, 1567
BsuTUI ATCGAT 1 cut(s) 2133
BtgI CCRYGG 1 cut(s) 1415
BtgZI GCGATG 1 cut(s) 211
BtrI CACGTC 1 cut(s) 703
BtsI GCAGTG 3 cut(s) 637, 2698, 3213
BtsIMutI CAGTG 4 cut(s) 637, 2698, 3195, 3213
Cac8I GCNNGC 7 cut(s) 1126, 1146, 1177, 2414, 2891, 3034, 3231
CfoI GCGC 2 cut(s) 2546, 3167
Cfr10I RCCGGY 3 cut(s) 291, 1784, 3235
Cfr13I GGNCC 5 cut(s) 294, 896, 1040, 1411, 3241
Cfr9I CCCGGG 1 cut(s) 1322
ClaI ATCGAT 1 cut(s) 2133
CpoI CGGWCCG 1 cut(s) 294
CseI GACGC 2 cut(s) 194, 3078
Csp6I GTAC 6 cut(s) 158, 935, 1713, 1923, 1992, 2271
CspAI ACCGGT 3 cut(s) 291, 1784, 3235
CspI CGGWCCG 1 cut(s) 294
CviQI GTAC 6 cut(s) 158, 935, 1713, 1923, 1992, 2271
DraI TTTAAA 4 cut(s) 426, 609, 1135, 2626
DrdI GACNNNNNNGTC 1 cut(s) 595
DriI GACNNNNNGTC 1 cut(s) 410
DseDI GACNNNNNNGTC 1 cut(s) 595
EaeI YGGCCR 1 cut(s) 1565
Eam1104I CTCTTC 3 cut(s) 2436, 2523, 3099
Eam1105I GACNNNNNGTC 1 cut(s) 410
EarI CTCTTC 3 cut(s) 2436, 2523, 3099
EciI GGCGGA 1 cut(s) 325
Eco130I CCWWGG 3 cut(s) 946, 1230, 1415
Eco147I AGGCCT 1 cut(s) 951
Eco32I GATATC 1 cut(s) 2923
Eco47I GGWCC 4 cut(s) 294, 896, 1040, 3241
Eco81I CCTNAGG 1 cut(s) 1044
Eco88I CYCGRG 2 cut(s) 339, 1322
EcoO109I RGGNCCY 2 cut(s) 1040, 1411
EcoRI GAATTC 1 cut(s) 768
EcoRII CCWGG 3 cut(s) 912, 1801, 2686
EcoRV GATATC 1 cut(s) 2923
EcoT14I CCWWGG 3 cut(s) 946, 1230, 1415
EcoT22I ATGCAT 3 cut(s) 883, 1146, 1266
ErhI CCWWGG 3 cut(s) 946, 1230, 1415
FalI AAGNNNNNCTT 2 cut(s) 576, 608
FaqI GGGAC 3 cut(s) 341, 481, 3227
FauNDI CATATG 2 cut(s) 358, 461
FbaI TGATCA 2 cut(s) 202, 637
FblI GTMKAC 2 cut(s) 403, 825
Fnu4HI GCNGC 8 cut(s) 258, 1149, 1333, 2475, 2833, 3087, 3163, 3219
Fsp4HI GCNGC 8 cut(s) 258, 1149, 1333, 2475, 2833, 3087, 3163, 3219
FspBI CTAG 8 cut(s) 572, 714, 1169, 2471, 2525, 2540, 2937, 3273
GlaI GCGC 2 cut(s) 2545, 3166
GluI GCNGC 8 cut(s) 258, 1149, 1333, 2475, 2833, 3087, 3163, 3219
GsaI CCCAGC 2 cut(s) 761, 2980
GsuI CTGGAG 1 cut(s) 1542
HaeII RGCGCY 1 cut(s) 2547
HaeIII GGCC 3 cut(s) 951, 1412, 1567
HapII CCGG 5 cut(s) 292, 1323, 1785, 3222, 3236
HgaI GACGC 2 cut(s) 194, 3078
HhaI GCGC 2 cut(s) 2546, 3167
Hin1I GRCGYC 1 cut(s) 3089
Hin6I GCGC 2 cut(s) 2544, 3165
HinP1I GCGC 2 cut(s) 2544, 3165
HincII GTYRAC 2 cut(s) 1360, 3052
HindII GTYRAC 2 cut(s) 1360, 3052
HindIII AAGCTT 6 cut(s) 855, 1173, 1450, 1469, 2315, 2414
HpaII CCGG 5 cut(s) 292, 1323, 1785, 3222, 3236
HphI GGTGA 6 cut(s) 194, 637, 1210, 1302, 3031, 3171
Hpy166II GTNNAC 8 cut(s) 349, 404, 826, 1360, 1992, 2271, 3052, 3278
Hpy8I GTNNAC 8 cut(s) 349, 404, 826, 1360, 1992, 2271, 3052, 3278
Hpy99I CGWCG 3 cut(s) 1367, 2617, 3094
HpyCH4III ACNGT 3 cut(s) 851, 2571, 2784
HpyCH4IV ACGT 3 cut(s) 702, 793, 1365
HpyF10VI GCNNNNNNNGC 5 cut(s) 456, 929, 1564, 2314, 3316
HpySE526I ACGT 3 cut(s) 702, 793, 1365
Hsp92I GRCGYC 1 cut(s) 3089
HspAI GCGC 2 cut(s) 2544, 3165
Ksp22I TGATCA 2 cut(s) 202, 637
LguI GCTCTTC 1 cut(s) 3099
LmnI GCTCC 3 cut(s) 151, 983, 3071
Lsp1109I GCAGC 6 cut(s) 269, 1160, 1344, 2486, 2819, 3174
LweI GCATC 8 cut(s) 234, 657, 1072, 1251, 2506, 2609, 2921, 2999
MaeI CTAG 8 cut(s) 572, 714, 1169, 2471, 2525, 2540, 2937, 3273
MaeII ACGT 3 cut(s) 702, 793, 1365
MaeIII GTNAC 6 cut(s) 182, 1308, 1831, 2038, 2179, 2320
MfeI CAATTG 4 cut(s) 384, 492, 1214, 3099
MflI RGATCY 6 cut(s) 1505, 1768, 1978, 2257, 2674, 2749
MlsI TGGCCA 1 cut(s) 1567
MluNI TGGCCA 1 cut(s) 1567
MlyI GAGTC 8 cut(s) 57, 404, 654, 1316, 1648, 2723, 2959, 2999
MmeI TCCRAC 7 cut(s) 1566, 1746, 1956, 2235, 2727, 2879, 2898
Mox20I TGGCCA 1 cut(s) 1567
Mph1103I ATGCAT 3 cut(s) 883, 1146, 1266
MroXI GAANNNNTTC 2 cut(s) 881, 1110
MscI TGGCCA 1 cut(s) 1567
MslI CAYNNNNRTG 2 cut(s) 1499, 1657
Msp20I TGGCCA 1 cut(s) 1567
MspA1I CMGCKG 1 cut(s) 2928
MspI CCGG 5 cut(s) 292, 1323, 1785, 3222, 3236
MspR9I CCNGG 6 cut(s) 914, 1323, 1324, 1803, 2688, 3223
MunI CAATTG 4 cut(s) 384, 492, 1214, 3099
Mva1269I GAATGC 4 cut(s) 881, 883, 1404, 1447
MvaI CCWGG 3 cut(s) 914, 1803, 2688
MvnI CGCG 1 cut(s) 3167
MwoI GCNNNNNNNGC 5 cut(s) 456, 929, 1564, 2314, 3316
NciI CCSGG 3 cut(s) 1323, 1324, 3223
NcoI CCATGG 1 cut(s) 1415
NdeI CATATG 2 cut(s) 358, 461
NlaIV GGNNCC 6 cut(s) 147, 979, 1413, 1800, 2148, 3243
NmuCI GTSAC 2 cut(s) 182, 1308
NsiI ATGCAT 3 cut(s) 883, 1146, 1266
NspI RCATGY 4 cut(s) 908, 932, 1148, 1695
PaeI GCATGC 1 cut(s) 1148
PceI AGGCCT 1 cut(s) 951
PciI ACATGT 1 cut(s) 1691
PciSI GCTCTTC 1 cut(s) 3099
PctI GAATGC 4 cut(s) 881, 883, 1404, 1447
PdmI GAANNNNTTC 2 cut(s) 881, 1110
PflMI CCANNNNNTGG 1 cut(s) 2693
PinAI ACCGGT 3 cut(s) 291, 1784, 3235
PkrI GCNGC 8 cut(s) 259, 1150, 1334, 2476, 2834, 3088, 3164, 3220
PleI GAGTC 8 cut(s) 57, 403, 653, 1315, 1647, 2722, 2959, 2998
PpsI GAGTC 8 cut(s) 57, 403, 653, 1315, 1647, 2722, 2959, 2998
PpuMI RGGWCCY 1 cut(s) 1040
PscI ACATGT 1 cut(s) 1691
PsiI TTATAA 1 cut(s) 656
Psp1406I AACGTT 1 cut(s) 793
Psp5II RGGWCCY 1 cut(s) 1040
Psp6I CCWGG 3 cut(s) 912, 1801, 2686
PspFI CCCAGC 2 cut(s) 757, 2976
PspGI CCWGG 3 cut(s) 912, 1801, 2686
PspN4I GGNNCC 6 cut(s) 147, 979, 1413, 1800, 2148, 3243
PspPI GGNCC 5 cut(s) 294, 896, 1040, 1411, 3241
PspPPI RGGWCCY 1 cut(s) 1040
PstI CTGCAG 1 cut(s) 101
PsuI RGATCY 6 cut(s) 1505, 1768, 1978, 2257, 2674, 2749
RsaI GTAC 6 cut(s) 159, 936, 1714, 1924, 1993, 2272
RsaNI GTAC 6 cut(s) 158, 935, 1713, 1923, 1992, 2271
RseI CAYNNNNRTG 2 cut(s) 1499, 1657
Rsr2I CGGWCCG 1 cut(s) 294
RsrII CGGWCCG 1 cut(s) 294
SapI GCTCTTC 1 cut(s) 3099
SatI GCNGC 8 cut(s) 258, 1149, 1333, 2475, 2833, 3087, 3163, 3219
Sau96I GGNCC 5 cut(s) 294, 896, 1040, 1411, 3241
SchI GAGTC 8 cut(s) 57, 404, 654, 1316, 1648, 2723, 2959, 2999
ScrFI CCNGG 6 cut(s) 914, 1323, 1324, 1803, 2688, 3223
SfaNI GCATC 8 cut(s) 234, 657, 1072, 1251, 2506, 2609, 2921, 2999
SfcI CTRYAG 2 cut(s) 97, 2385
SgrAI CRCCGGYG 1 cut(s) 3235
SinI GGWCC 4 cut(s) 294, 896, 1040, 3241
SmaI CCCGGG 1 cut(s) 1324
SmiMI CAYNNNNRTG 2 cut(s) 1499, 1657
SmlI CTYRAG 8 cut(s) 248, 1879, 1948, 2086, 2227, 2417, 2455, 2668
SmoI CTYRAG 8 cut(s) 248, 1879, 1948, 2086, 2227, 2417, 2455, 2668
SpeI ACTAGT 2 cut(s) 2936, 3272
SphI GCATGC 1 cut(s) 1148
SseBI AGGCCT 1 cut(s) 951
SsiI CCGC 6 cut(s) 336, 2928, 3086, 3160, 3167, 3219
SspI AATATT 3 cut(s) 442, 1630, 2578
SspMI CTAG 8 cut(s) 572, 714, 1169, 2471, 2525, 2540, 2937, 3273
StuI AGGCCT 1 cut(s) 951
StyD4I CCNGG 6 cut(s) 912, 1321, 1322, 1801, 2686, 3221
StyI CCWWGG 3 cut(s) 946, 1230, 1415
TaaI ACNGT 3 cut(s) 851, 2571, 2784
TaiI ACGT 3 cut(s) 705, 796, 1368
TaqI TCGA 7 cut(s) 432, 1430, 1813, 2020, 2133, 2161, 2880
TatI WGTACW 2 cut(s) 1712, 1922
TauI GCSGC 2 cut(s) 3089, 3221
TscAI CASTG 4 cut(s) 637, 2698, 3195, 3220
TseFI GTSAC 2 cut(s) 182, 1308
TseI GCWGC 6 cut(s) 257, 1148, 1332, 2474, 2832, 3162
Tsp45I GTSAC 2 cut(s) 182, 1308
TspDTI ATGAA 8 cut(s) 345, 834, 1332, 1478, 1592, 1811, 2018, 2159
TspGWI ACGGA 4 cut(s) 46, 1566, 2490, 3105
TspMI CCCGGG 1 cut(s) 1322
TspRI CASTG 4 cut(s) 637, 2698, 3195, 3220
Van91I CCANNNNNTGG 1 cut(s) 2693
VpaK11BI GGWCC 4 cut(s) 294, 896, 1040, 3241
XceI RCATGY 4 cut(s) 908, 932, 1148, 1695
XmaI CCCGGG 1 cut(s) 1322
XmiI GTMKAC 2 cut(s) 403, 825
XmnI GAANNNNTTC 2 cut(s) 881, 1110
XspI CTAG 8 cut(s) 572, 714, 1169, 2471, 2525, 2540, 2937, 3273
Zsp2I ATGCAT 3 cut(s) 883, 1146, 1266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.