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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 143 |
| AciI | CCGC | 2 cut(s) | 125, 141 |
| AclWI | GGATC | 1 cut(s) | 509 |
| AcsI | RAATTY | 1 cut(s) | 153 |
| AfaI | GTAC | 1 cut(s) | 132 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 237, 322 |
| AgsI | TTSAA | 3 cut(s) | 163, 214, 449 |
| AleI | CACNNNNGTG | 1 cut(s) | 99 |
| AluBI | AGCT | 3 cut(s) | 404, 417, 495 |
| AluI | AGCT | 3 cut(s) | 404, 417, 495 |
| Alw21I | GWGCWC | 1 cut(s) | 465 |
| Alw26I | GTCTC | 1 cut(s) | 245 |
| AlwI | GGATC | 1 cut(s) | 509 |
| AoxI | GGCC | 2 cut(s) | 320, 483 |
| ApeKI | GCWGC | 2 cut(s) | 145, 495 |
| ApoI | RAATTY | 1 cut(s) | 153 |
| AspLEI | GCGC | 1 cut(s) | 145 |
| AspS9I | GGNCC | 1 cut(s) | 483 |
| AsuC2I | CCSGG | 2 cut(s) | 232, 487 |
| AsuHPI | GGTGA | 1 cut(s) | 86 |
| Bbv12I | GWGCWC | 1 cut(s) | 465 |
| BbvI | GCAGC | 2 cut(s) | 132, 482 |
| BccI | CCATC | 1 cut(s) | 340 |
| BceAI | ACGGC | 1 cut(s) | 21 |
| BclI | TGATCA | 1 cut(s) | 78 |
| BcnI | CCSGG | 2 cut(s) | 232, 487 |
| BcoDI | GTCTC | 1 cut(s) | 245 |
| BfaI | CTAG | 2 cut(s) | 365, 401 |
| BglII | AGATCT | 1 cut(s) | 55 |
| BisI | GCNGC | 2 cut(s) | 146, 496 |
| BlsI | GCNGC | 2 cut(s) | 147, 497 |
| Bme1390I | CCNGG | 2 cut(s) | 232, 487 |
| BmgT120I | GGNCC | 1 cut(s) | 483 |
| BmiI | GGNNCC | 1 cut(s) | 139 |
| BmrFI | CCNGG | 2 cut(s) | 232, 487 |
| BmrI | ACTGGG | 1 cut(s) | 490 |
| BmuI | ACTGGG | 1 cut(s) | 490 |
| BpuMI | CCSGG | 2 cut(s) | 232, 487 |
| BsaAI | YACGTR | 1 cut(s) | 134 |
| BsaJI | CCNNGG | 1 cut(s) | 429 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 237, 322 |
| Bse1I | ACTGG | 1 cut(s) | 485 |
| BseDI | CCNNGG | 1 cut(s) | 429 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 237, 322 |
| BseNI | ACTGG | 1 cut(s) | 485 |
| BseXI | GCAGC | 2 cut(s) | 132, 482 |
| Bsh1236I | CGCG | 1 cut(s) | 143 |
| BshFI | GGCC | 2 cut(s) | 322, 485 |
| BsiHKAI | GWGCWC | 1 cut(s) | 465 |
| BsiSI | CCGG | 3 cut(s) | 232, 323, 486 |
| BslFI | GGGAC | 1 cut(s) | 375 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 237, 322 |
| BsmAI | GTCTC | 1 cut(s) | 245 |
| BsmFI | GGGAC | 1 cut(s) | 375 |
| BsnI | GGCC | 2 cut(s) | 322, 485 |
| Bsp1286I | GDGCHC | 1 cut(s) | 465 |
| Bsp143I | GATC | 3 cut(s) | 55, 78, 501 |
| Bsp19I | CCATGG | 1 cut(s) | 429 |
| BspACI | CCGC | 2 cut(s) | 125, 141 |
| BspANI | GGCC | 2 cut(s) | 322, 485 |
| BspFNI | CGCG | 1 cut(s) | 143 |
| BspLI | GGNNCC | 1 cut(s) | 139 |
| BspPI | GGATC | 1 cut(s) | 509 |
| BspQI | GCTCTTC | 1 cut(s) | 411 |
| BsrI | ACTGG | 1 cut(s) | 485 |
| BssECI | CCNNGG | 1 cut(s) | 429 |
| BssMI | GATC | 3 cut(s) | 55, 78, 501 |
| BssT1I | CCWWGG | 1 cut(s) | 429 |
| Bst4CI | ACNGT | 3 cut(s) | 22, 169, 467 |
| Bst6I | CTCTTC | 2 cut(s) | 411, 465 |
| BstBAI | YACGTR | 1 cut(s) | 134 |
| BstDSI | CCRYGG | 1 cut(s) | 429 |
| BstFNI | CGCG | 1 cut(s) | 143 |
| BstHHI | GCGC | 1 cut(s) | 145 |
| BstKTI | GATC | 3 cut(s) | 58, 81, 504 |
| BstMAI | GTCTC | 1 cut(s) | 245 |
| BstMBI | GATC | 3 cut(s) | 55, 78, 501 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 14 |
| BstNSI | RCATGY | 1 cut(s) | 203 |
| BstSCI | CCNGG | 2 cut(s) | 230, 485 |
| BstUI | CGCG | 1 cut(s) | 143 |
| BstV1I | GCAGC | 2 cut(s) | 132, 482 |
| BstX2I | RGATCY | 1 cut(s) | 55 |
| BstYI | RGATCY | 1 cut(s) | 55 |
| BsuRI | GGCC | 2 cut(s) | 322, 485 |
| BtgI | CCRYGG | 1 cut(s) | 429 |
| BtsI | GCAGTG | 1 cut(s) | 192 |
| BtsIMutI | CAGTG | 3 cut(s) | 165, 192, 463 |
| CfoI | GCGC | 1 cut(s) | 145 |
| Cfr13I | GGNCC | 1 cut(s) | 483 |
| Csp6I | GTAC | 1 cut(s) | 131 |
| CviAII | CATG | 3 cut(s) | 200, 383, 430 |
| CviJI | RGCY | 9 cut(s) | 47, 138, 265, 322, 404, 417, 485, 495, 527 |
| CviKI_1 | RGCY | 9 cut(s) | 47, 138, 265, 322, 404, 417, 485, 495, 527 |
| CviQI | GTAC | 1 cut(s) | 131 |
| DpnI | GATC | 3 cut(s) | 57, 80, 503 |
| DpnII | GATC | 3 cut(s) | 55, 78, 501 |
| Eam1104I | CTCTTC | 2 cut(s) | 411, 465 |
| EarI | CTCTTC | 2 cut(s) | 411, 465 |
| Eco130I | CCWWGG | 1 cut(s) | 429 |
| EcoT14I | CCWWGG | 1 cut(s) | 429 |
| ErhI | CCWWGG | 1 cut(s) | 429 |
| FaeI | CATG | 3 cut(s) | 203, 386, 433 |
| FaiI | YATR | 5 cut(s) | 201, 209, 299, 384, 431 |
| FaqI | GGGAC | 1 cut(s) | 375 |
| FatI | CATG | 3 cut(s) | 199, 382, 429 |
| FbaI | TGATCA | 1 cut(s) | 78 |
| Fnu4HI | GCNGC | 2 cut(s) | 146, 496 |
| Fsp4HI | GCNGC | 2 cut(s) | 146, 496 |
| FspBI | CTAG | 2 cut(s) | 365, 401 |
| GlaI | GCGC | 1 cut(s) | 144 |
| GluI | GCNGC | 2 cut(s) | 146, 496 |
| HaeIII | GGCC | 2 cut(s) | 322, 485 |
| HapII | CCGG | 3 cut(s) | 232, 323, 486 |
| HhaI | GCGC | 1 cut(s) | 145 |
| Hin1II | CATG | 3 cut(s) | 203, 386, 433 |
| Hin6I | GCGC | 1 cut(s) | 143 |
| HinP1I | GCGC | 1 cut(s) | 143 |
| HinfI | GANTC | 1 cut(s) | 293 |
| HpaII | CCGG | 3 cut(s) | 232, 323, 486 |
| HphI | GGTGA | 1 cut(s) | 86 |
| Hpy188I | TCNGA | 1 cut(s) | 31 |
| Hpy188III | TCNNGA | 2 cut(s) | 192, 226 |
| HpyAV | CCTTC | 3 cut(s) | 4, 65, 295 |
| HpyCH4III | ACNGT | 3 cut(s) | 22, 169, 467 |
| HpyCH4IV | ACGT | 1 cut(s) | 133 |
| HpyCH4V | TGCA | 4 cut(s) | 17, 62, 199, 283 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 14 |
| HpySE526I | ACGT | 1 cut(s) | 133 |
| Hsp92II | CATG | 3 cut(s) | 203, 386, 433 |
| HspAI | GCGC | 1 cut(s) | 143 |
| Ksp22I | TGATCA | 1 cut(s) | 78 |
| Kzo9I | GATC | 3 cut(s) | 55, 78, 501 |
| LguI | GCTCTTC | 1 cut(s) | 411 |
| LmnI | GCTCC | 1 cut(s) | 143 |
| Lsp1109I | GCAGC | 2 cut(s) | 132, 482 |
| MaeI | CTAG | 2 cut(s) | 365, 401 |
| MaeII | ACGT | 1 cut(s) | 133 |
| MalI | GATC | 3 cut(s) | 57, 80, 503 |
| MboI | GATC | 3 cut(s) | 55, 78, 501 |
| MboII | GAAGA | 2 cut(s) | 398, 482 |
| MfeI | CAATTG | 1 cut(s) | 510 |
| MflI | RGATCY | 1 cut(s) | 55 |
| MhlI | GDGCHC | 1 cut(s) | 465 |
| MluCI | AATT | 4 cut(s) | 153, 442, 449, 510 |
| MlyI | GAGTC | 1 cut(s) | 302 |
| MmeI | TCCRAC | 1 cut(s) | 54 |
| MnlI | CCTC | 5 cut(s) | 37, 61, 166, 265, 343 |
| MslI | CAYNNNNRTG | 1 cut(s) | 99 |
| MspI | CCGG | 3 cut(s) | 232, 323, 486 |
| MspR9I | CCNGG | 2 cut(s) | 232, 487 |
| MunI | CAATTG | 1 cut(s) | 510 |
| MvnI | CGCG | 1 cut(s) | 143 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 14 |
| NciI | CCSGG | 2 cut(s) | 232, 487 |
| NcoI | CCATGG | 1 cut(s) | 429 |
| NdeII | GATC | 3 cut(s) | 55, 78, 501 |
| NlaIII | CATG | 3 cut(s) | 203, 386, 433 |
| NlaIV | GGNNCC | 1 cut(s) | 139 |
| NspI | RCATGY | 1 cut(s) | 203 |
| OliI | CACNNNNGTG | 1 cut(s) | 99 |
| PciSI | GCTCTTC | 1 cut(s) | 411 |
| PkrI | GCNGC | 2 cut(s) | 147, 497 |
| PleI | GAGTC | 1 cut(s) | 301 |
| PpsI | GAGTC | 1 cut(s) | 301 |
| Ppu21I | YACGTR | 1 cut(s) | 134 |
| PspN4I | GGNNCC | 1 cut(s) | 139 |
| PspPI | GGNCC | 1 cut(s) | 483 |
| PsuI | RGATCY | 1 cut(s) | 55 |
| RsaI | GTAC | 1 cut(s) | 132 |
| RsaNI | GTAC | 1 cut(s) | 131 |
| RseI | CAYNNNNRTG | 1 cut(s) | 99 |
| SapI | GCTCTTC | 1 cut(s) | 411 |
| SatI | GCNGC | 2 cut(s) | 146, 496 |
| Sau3AI | GATC | 3 cut(s) | 55, 78, 501 |
| Sau96I | GGNCC | 1 cut(s) | 483 |
| SchI | GAGTC | 1 cut(s) | 302 |
| ScrFI | CCNGG | 2 cut(s) | 232, 487 |
| SduI | GDGCHC | 1 cut(s) | 465 |
| SetI | ASST | 7 cut(s) | 99, 136, 177, 354, 406, 419, 497 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 99 |
| Sse9I | AATT | 4 cut(s) | 153, 442, 449, 510 |
| SsiI | CCGC | 2 cut(s) | 125, 141 |
| SspMI | CTAG | 2 cut(s) | 365, 401 |
| StyD4I | CCNGG | 2 cut(s) | 230, 485 |
| StyI | CCWWGG | 1 cut(s) | 429 |
| TaaI | ACNGT | 3 cut(s) | 22, 169, 467 |
| TaiI | ACGT | 1 cut(s) | 136 |
| TaqI | TCGA | 1 cut(s) | 344 |
| TasI | AATT | 4 cut(s) | 153, 442, 449, 510 |
| TscAI | CASTG | 3 cut(s) | 172, 192, 470 |
| TseI | GCWGC | 2 cut(s) | 145, 495 |
| TspDTI | ATGAA | 1 cut(s) | 314 |
| TspRI | CASTG | 3 cut(s) | 172, 192, 470 |
| XapI | RAATTY | 1 cut(s) | 153 |
| XceI | RCATGY | 1 cut(s) | 203 |
| XspI | CTAG | 2 cut(s) | 365, 401 |