Rorug06G0025400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000123
Physical Location & Seq
Reverse (-)
3118766 .. 3122580
3815 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0025400.1

Sequence Viewer

Length: 3333 bp
ATGAGCACACAAAGAACCTCTGCATCTTTCCCCCCTTCATCTCATCAGTGGAAATATGATGTTTTTCTGAGCTTTAGAGGTGACGACACTCGGAAGACTTTTACAGACCACTTATTCACTGCATTGGAACGTCAAGGAATCATAACTTTCAGGGATGATCCAGAACTTCAAAAAGGGGAAGCTATTTCTCCAGCACTTTTTGCTGCAATCGAAGAATCGAGATTTGCTCTCATTGTTATGTCACAAAATTATGCATCGTCAACATGGTGCTTGGATGAACTTGTGAGAATTCTTGAATGCATGAAAGCAAGAGGAACTGTGCTGCCAATTTTCTATGATGTTGATCCGTCTGATGTACGAAAGCAAACAGGGAGTTTTAAAAGAGCCTTTGCAAGCCATGAAGAAAGGTTTAAGGATGACAAAGAGAAAGTGCAGAGGTGGAGATCTGCATTAACTGAAGTGGCAAGTTTCTCTGGGTGGAATTCAAAGGAATGGTATGAATCAAAGCTCATTAGAGATATTGTTGAAGTTATATGTAAAGGATTGCAACCTACATCATACAGTTATGCAGAAAATCTAGTTGGAATTTACTCAAGATTGCATCGAGTTAATTTGCTTTTAAGTGTAGGGGTGAATGACGTCCGCTTCATTGGGATATGGGGAATGGGCGGGATTGGTAAGACAACTATGGTAAGAGTGGTGCATGAGAGAATCTCTCATGAATTTGAATTTAATTTCCTTCTTACCGATGTTAGAAACTCCGTTGAAAAAGGTGGTTTACTTAATCTGCAAAAGCAACTTCTCTCTGGGATTGGGGCAAGAAAGGCTGACATATTAGACCTTCAGGAAGGAGCCACCATATTAAAGAGGTTATTACGTCACAAAAAAGTTCTTCTCATCCTTGATGATGTGAACCATTCAAGTCATTTAGAATATTTGGCAGGAAACCCAGAGTGGTTTGGTTCAGGGAGTAGAGTTCTTATCACAACTAGAGATGAGCATTTGTTGATTGAACGTGAAGTGGAAAGAAGATTGAAGGTCGAAGAATTAAATGATGATGATTCTCTTCACCTTTTTAGCTGGAAAGCTTTCAAAAGAGGTTACCCTGAAGAAGATTTTCTTGATTTGTCTAAATCTGTTATAAGTTATGCCAAAGGTCTTCCTTTAGCTCTTAAAGTACTGGGTTCTCTTTTGTATAAAAGTGATCCAAGTGAATGGAACAGTGCATTGAGAAAACTGGGAAGAGTTTGTAACTCGGAAGTCTTTGACATACTTAAAATAAGTTACGACGGTCTAGATTCTGAAGAGAAGAAAATATTCCTAGACATTGCATGTTTCTTTAATTGGCAAGACAAAGATCGAGTAACAGAAGTACTAACCAGTTGCGATTTTTCTGCAATTTTTGGAATAAAAGTTCTTACCGAAAGATCTCTCTTGACTGTTTCACATGAAAGACTAGGGATGCACGATTTGCTCCAAGAAATGGGATGGGAAATTGTCCGCCGGGAATCTCCTAACGAGCTGGGCAGGTGCAGTAGGTTGTGGCTTCTTGAAGACGTCAAACATGTCTTGACCAAAAATACTGGAACTGAAGCAATAGAAGGCATATTTGTGGACTCTACAGAGTTGGGAGTAAAGGTGGACGTGACTCCGAAATCATTTTCAATGATGAACAAATTGAGATACCTGAAGATTGAGAATGGGAACCTACCTAAGGGACTTGAATATCTTCCCAATAGTTTACGGATTCTTGATTGGACGAGGTATCCGTTAAAATCTTTGCCATCACATTTCAACCCTCAGAAGCTCCTCGAACTAAGCTTGTCTCATAGTTGTTTTAAACATTTTCGGATAGGAACAGAGCGTTTATGCAATTTGATAACCATTAATCTGAGTCACTCTCTAAATCTTGTCAACACCCCAAACTTTAAAGATATGCCGTATCTCAAATTTCTGGTTCTTGAAGGTTGTGTAAGATTGTATGAGGTTGACGCAGGAATCGAAGTGCTTGAAAGACTTACTGTGCTGAACTTGAAAGATTGCAAGAATCTTGTGCGACTTCCAAGCAGTGTAGGTGGCTTAAAATCTCTCAAAGATCTCAATCTTTCTGGTTGCTCAAAGCTTGACAAATTGCCGGATGAGTTGGGTCATGTTGCCTGTTTGGAGAAGCTTGATGTGAGTGGAAGTGGCATAAGAGAACTGCCCTCCTCTATTGGTCTTTTGAAAAACCTCAAAGAATTCTCTCTTGCTGGGTGTAAAGCAGAGTCACCTAAATCATGGAATCTGATCCCCTTTCAGTTATTGCGAAAAAGAAGTCACATTCCGGCAGGATTGTCTTTGCCTTGTTTGTCGGGTTTGCATTCATTAACTACATTGAATCTAAGTGACTGCAATCTTTCTGAAGAAGCAATGCCCAGTGATTTTGGATGCTTGTCTTCATTAAGAGAATTAAATCTCAGCGGAAATCAATTCAGTAGACTACCAGAGCTTCCATCTCATCATGTATGGGTAAACGCTGACAACTGCATTTCATTAGATACGTTGGCCAATCAAATAGGACAAAGCAATTCGTTTGTTAACTGTTTCAAACTGGTGGAGAATCAAAGCTGTGAGAGTATAGCACTTTCATGGCTAACACGCTACCTTAAGAGTCAACCATTTTCTGACCATGAAGAATTTGACTTGGTTGTTCCTGGAAATGAAATTCCAGAGTGGTGCGACTTAGTTGTTCCTGGAAATGAAATTCCAGAGTGGTACAATCACCGAAGCGTGGGGTCTTCGATAACCGTAGAGCTGCATCCAGGTTGGTTTAGTAACAAGTGGATGGGATTCGCCTGTTGTGTCGTTTTTAGACTCCTCAAACCACTGCCGCCTTCTGTTCTGTGGTCCATTGATTGCGAACTGAGTGCCAATGGAAAATCCTTGCCTTATGGTGCTATAATAGTGTTCGGGGGAAACTGTCAACCTGTGTTGGATCACATTTGGTTCTTCAGTGGGGCGCGTGATATATTCTCTCAACATGAGTGGCAGGATATCTACTGTCAGCTTGTATTCTCATTTAATATCAACTTGGACTTGGATGAGGAAATTGTGCAAGTGCAGAAGTGTGGGGTCCGTATGATATATGAGGAAGATGCAGACGAGCTTGCGGAAACATTATTGAAGCAGAGCAATGTTGGCATTAATACCAAGCGAGGCCTTCAACACTATAATGGTGATGATGCAGCATCTAGTAGTACAAGTCAGGCTCATCCAAAAAGAATGAAACAACTCCACATTGACGGTGCAGGACCCAGTAGAACCCCTTATATTCATGACCAGAAATATGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1110

Amino Acids

125.76

Weight (kDa)

6.06

Isoelectric Point (pI)

42.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 18 - 193 8.4e-54 TIR domain
TIR_2 PF13676 21 - 117 1.8e-14 TIR domain
NB-ARC PF00931 212 - 366 3.4e-20 NB-ARC domain
WHD_ROQ1 PF23282 435 - 504 8.3e-18 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 673 - 750 7.5e-11 Leucine-rich repeat region
C-JID PF20160 910 - 1046 1.6e-18 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000123)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G38140
fragaria_vesca FvH4_1g20041 FvH4_1g21262 FvH4_1g25072 FvH4_2g16250 FvH4_2g17190 FvH4_3g04330 FvH4_3g20250 FvH4_3g22272 FvH4_3g26421 FvH4_3g35981 FvH4_4g01621 FvH4_4g07081 FvH4_4g13902 FvH4_4g18461 FvH4_5g15851 FvH4_6g06531 FvH4_6g22500 FvH4_6g23161 FvH4_6g26861 FvH4_6g28682 FvH4_6g33163 FvH4_6g33591 FvH4_6g38140 FvH4_7g00272
malus_domestica MD05G1145500.v1.1 MD10G1144900.v1.1
prunus_persica Prupe.8G183300_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0109311 RchiOBHm_Chr2g0109451 RchiOBHm_Chr2g0164071 RchiOBHm_Chr3g0469891 RchiOBHm_Chr3g0477271 RchiOBHm_Chr6g0281681
rosa_laevigata RLG00000007350 RLG00000011905 RLG00000012951 RLG00000030154
rosa_multiflora Rmu_co8029630.1_g000001 Rmu_co8038194.1_g000001 Rmu_co8114330.1_g000001 Rmu_co8138832.1_g000001 Rmu_co8166524.1_g000001 Rmu_co8169592.1_g000001 Rmu_co8214120.1_g000001 Rmu_co8224140.1_g000001 Rmu_co8242461.1_g000001 Rmu_co8314423.1_g000002 Rmu_co8333117.1_g000001 Rmu_co8348539.1_g000002 Rmu_co8348927.1_g000001 Rmu_co8416111.1_g000001 Rmu_co8444475.1_g000001 Rmu_co8503525.1_g000001 Rmu_sc0000047.1_g000017 Rmu_sc0000076.1_g000047 Rmu_sc0000116.1_g000002 Rmu_sc0000146.1_g000004 Rmu_sc0000239.1_g000065 Rmu_sc0000335.1_g000105 Rmu_sc0000424.1_g000035 Rmu_sc0000517.1_g000021 Rmu_sc0000611.1_g000003 Rmu_sc0000770.1_g000050 Rmu_sc0000808.1_g000032 Rmu_sc0000811.1_g000026 Rmu_sc0000894.1_g000054 Rmu_sc0000898.1_g000082 Rmu_sc0000898.1_g000083 Rmu_sc0001350.1_g000029 Rmu_sc0001374.1_g000062 Rmu_sc0001405.1_g000018 Rmu_sc0001474.1_g000014 Rmu_sc0001526.1_g000021 Rmu_sc0001551.1_g000029 Rmu_sc0001557.1_g000004 Rmu_sc0001650.1_g000005 Rmu_sc0001650.1_g000013 Rmu_sc0001662.1_g000001 Rmu_sc0001673.1_g000030 Rmu_sc0001701.1_g000021 Rmu_sc0001814.1_g000019 Rmu_sc0002038.1_g000007 Rmu_sc0002179.1_g000011 Rmu_sc0002285.1_g000020 Rmu_sc0002575.1_g000023 Rmu_sc0002731.1_g000001 Rmu_sc0002944.1_g000021 Rmu_sc0003023.1_g000002 Rmu_sc0003129.1_g000006 Rmu_sc0003144.1_g000013 Rmu_sc0003156.1_g000010 Rmu_sc0003369.1_g000015 Rmu_sc0003410.1_g000028 Rmu_sc0003410.1_g000029 Rmu_sc0003507.1_g000033 Rmu_sc0003548.1_g000013 Rmu_sc0003605.1_g000027 Rmu_sc0003710.1_g000010 Rmu_sc0003863.1_g000009 Rmu_sc0003909.1_g000006 Rmu_sc0003916.1_g000023 Rmu_sc0003916.1_g000024 Rmu_sc0003930.1_g000009 Rmu_sc0004030.1_g000006 Rmu_sc0004046.1_g000018 Rmu_sc0004154.1_g000010 Rmu_sc0004191.1_g000011 Rmu_sc0004205.1_g000007 Rmu_sc0004556.1_g000006 Rmu_sc0004975.1_g000008 Rmu_sc0005038.1_g000028 Rmu_sc0005083.1_g000005 Rmu_sc0005220.1_g000016 Rmu_sc0005274.1_g000011 Rmu_sc0005378.1_g000023 Rmu_sc0005385.1_g000016 Rmu_sc0005424.1_g000023 Rmu_sc0005738.1_g000009 Rmu_sc0005798.1_g000015 Rmu_sc0005834.1_g000008 Rmu_sc0006128.1_g000007 Rmu_sc0006865.1_g000015 Rmu_sc0006925.1_g000001 Rmu_sc0007005.1_g000002 Rmu_sc0007025.1_g000017 Rmu_sc0007518.1_g000004 Rmu_sc0007813.1_g000002 Rmu_sc0008730.1_g000003 Rmu_sc0008861.1_g000008 Rmu_sc0008861.1_g000009 Rmu_sc0009624.1_g000009 Rmu_sc0010439.1_g000001 Rmu_sc0010719.1_g000003 Rmu_sc0011069.1_g000001 Rmu_sc0013102.1_g000007 Rmu_sc0013803.1_g000022 Rmu_sc0013860.1_g000004 Rmu_sc0014885.1_g000001 Rmu_sc0017338.1_g000001 Rmu_sc0017964.1_g000006 Rmu_sc0019100.1_g000002 Rmu_sc0020554.1_g000001 Rmu_sc0020928.1_g000001 Rmu_sc0021886.1_g000001 Rmu_sc0022276.1_g000001 Rmu_sc0022942.1_g000001 Rmu_sc0022945.1_g000001 Rmu_sc0023180.1_g000002 Rmu_sc0024149.1_g000001 Rmu_sc0024149.1_g000002 Rmu_sc0026250.1_g000001 Rmu_sc0029882.1_g000003 Rmu_sc0035589.1_g000001 Rmu_sc0035888.1_g000003 Rmu_sc0036257.1_g000001 Rmu_sc0036258.1_g000001 Rmu_sc0040283.1_g000002 Rmu_sc0042169.1_g000001 Rmu_ssc0000126.1_g000076 Rmu_ssc0000357.1_g000017 Rmu_ssc0000372.1_g000063 Rmu_ssc0000391.1_g000030 Rmu_ssc0000432.1_g000006 Rmu_ssc0000480.1_g000008
rosa_roxburghii Rroxscaffold_1G00047150 Rroxscaffold_2G00124620 Rroxscaffold_2G00130500 Rroxscaffold_2G00146610 Rroxscaffold_3G00260760 Rroxscaffold_3G00270900 Rroxscaffold_4G00282170 Rroxscaffold_6G00418750 Rroxscaffold_7G00186930
rosa_rugosa Rorug01G0267800 Rorug02G0132500 Rorug02G0156000 Rorug02G0163300 Rorug02G0282900 Rorug02G0283000 Rorug02G0439400 Rorug02G0488800 Rorug02G0488900 Rorug02G0556100 Rorug03G0101100 Rorug03G0101200 Rorug03G0130200 Rorug03G0223700 Rorug04G0015200 Rorug04G0032400 Rorug04G0043600 Rorug04G0049700 Rorug04G0137000 Rorug04G0178300 Rorug04G0198500 Rorug05G0298400 Rorug05G0438000 Rorug05G0499300 Rorug06G0025400 Rorug06G0142400 Rorug07G0108200 Rorug07G0111200
rosa_samantha Rh1CG412100 Rh2AG574700 Rh2BG495100 Rh2CG255400 Rh2CG263700 Rh2CG548300 Rh3BG288300 Rh3DG068500 Rh5CG272200 Rh5DG249700 Rh5DG397400 Rh6AG255100 Rh6BG258500 Rh6CG257300 Rh6DG249500 Rh6DG268300 Rh6DG381800 Rh7BG282800 Rh7BG372100
rosa_wichuraiana Rw4G024160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1142
AarI CACCTGC 1 cut(s) 1518
AatII GACGTC 2 cut(s) 642, 1560
Acc36I ACCTGC 1 cut(s) 1518
AccB7I CCANNNNNTGG 1 cut(s) 1483
AccI GTMKAC 1 cut(s) 2476
AccII CGCG 1 cut(s) 3001
AciI CCGC 6 cut(s) 643, 669, 1501, 2460, 2870, 3149
AclWI GGATC 5 cut(s) 152, 338, 1199, 2281, 2982
AcoI YGGCCR 1 cut(s) 2544
AcuI CTGAAG 8 cut(s) 477, 827, 1128, 1323, 1611, 1709, 2421, 2974
AcyI GRCGYC 2 cut(s) 639, 1557
AfaI GTAC 5 cut(s) 357, 1179, 1374, 2756, 3238
AfiI CCNNNNNNNGG 3 cut(s) 1483, 1714, 2770
AflII CTTAAG 1 cut(s) 2645
AflIII ACRYGT 1 cut(s) 1564
AjiI CACGTC 1 cut(s) 1645
AjnI CCWGG 3 cut(s) 2692, 2731, 2800
AjuI GAANNNNNNNTTGG 2 cut(s) 564, 596
AloI GAACNNNNNNTCC 2 cut(s) 1398, 1430
Alw21I GWGCWC 1 cut(s) 8
Alw26I GTCTC 1 cut(s) 1830
AlwI GGATC 5 cut(s) 152, 338, 1199, 2281, 2982
AoxI GGCC 2 cut(s) 2544, 3196
ApeKI GCWGC 4 cut(s) 203, 322, 2794, 3224
ArsI GACNNNNNNTTYG 2 cut(s) 2299, 2331
AseI ATTAAT 2 cut(s) 1887, 3183
Asp700I GAANNNNTTC 4 cut(s) 1088, 1116, 1316, 1728
AspLEI GCGC 1 cut(s) 3001
AspS9I GGNCC 3 cut(s) 2886, 3112, 3290
AsuC2I CCSGG 1 cut(s) 1505
AsuHPI GGTGA 6 cut(s) 92, 643, 1061, 2259, 2753, 3227
AvaII GGWCC 3 cut(s) 2886, 3112, 3290
AxyI CCTNAGG 1 cut(s) 1713
BalI TGGCCA 1 cut(s) 2546
BbsI GAAGAC 5 cut(s) 101, 1151, 1560, 2427, 2769
Bbv12I GWGCWC 1 cut(s) 8
BbvI GCAGC 4 cut(s) 190, 309, 2781, 3236
BccI CCATC 4 cut(s) 1482, 1792, 2500, 2818
BceAI ACGGC 1 cut(s) 1924
BcgI CGANNNNNNTGC 4 cut(s) 1376, 1410, 2888, 2922
BciT130I CCWGG 3 cut(s) 2694, 2733, 2802
BciVI GTATCC 1 cut(s) 1776
BcnI CCSGG 1 cut(s) 1505
BcoDI GTCTC 1 cut(s) 1830
BfaI CTAG 6 cut(s) 578, 990, 1295, 1322, 1457, 3231
BfmI CTRYAG 1 cut(s) 1620
BfrI CTTAAG 1 cut(s) 2645
BfuAI ACCTGC 1 cut(s) 1518
BfuI GTATCC 1 cut(s) 1776
BglII AGATCT 3 cut(s) 443, 1427, 2095
BisI GCNGC 5 cut(s) 204, 323, 2795, 2870, 3225
BlsI GCNGC 5 cut(s) 205, 324, 2796, 2871, 3226
BmcAI AGTACT 2 cut(s) 1179, 1374
Bme1390I CCNGG 4 cut(s) 1505, 2694, 2733, 2802
Bme18I GGWCC 3 cut(s) 2886, 3112, 3290
BmgBI CACGTC 1 cut(s) 1645
BmgT120I GGNCC 3 cut(s) 2886, 3112, 3290
BmiI GGNNCC 4 cut(s) 853, 1706, 3113, 3292
BmrFI CCNGG 4 cut(s) 1505, 2694, 2733, 2802
BmrI ACTGGG 4 cut(s) 1190, 1247, 2409, 3288
BmsI GCATC 9 cut(s) 32, 263, 610, 1452, 2417, 2806, 3124, 3211, 3236
BmuI ACTGGG 4 cut(s) 1190, 1247, 2409, 3288
BpiI GAAGAC 5 cut(s) 101, 1151, 1560, 2427, 2769
BplI GAGNNNNNCTC 8 cut(s) 211, 243, 698, 730, 700, 732, 1885, 1917
BpmI CTGGAG 1 cut(s) 174
BpuEI CTTGAG 1 cut(s) 577
BpuMI CCSGG 1 cut(s) 1505
BsaBI GATNNNNATC 2 cut(s) 342, 2100
BsaHI GRCGYC 2 cut(s) 639, 1557
BsaXI ACNNNNNCTCC 2 cut(s) 1623, 1653
Bsc4I CCNNNNNNNGG 3 cut(s) 1483, 1714, 2770
Bse1I ACTGG 7 cut(s) 1185, 1242, 1380, 1588, 2415, 2595, 3294
Bse21I CCTNAGG 1 cut(s) 1713
Bse3DI GCAATG 3 cut(s) 1326, 2415, 3178
Bse8I GATNNNNATC 2 cut(s) 342, 2100
BseBI CCWGG 3 cut(s) 2694, 2733, 2802
BseJI GATNNNNATC 2 cut(s) 342, 2100
BseLI CCNNNNNNNGG 3 cut(s) 1483, 1714, 2770
BseMI GCAATG 3 cut(s) 1326, 2415, 3178
BseMII CTCAG 5 cut(s) 59, 1814, 1883, 2470, 2894
BseNI ACTGG 7 cut(s) 1185, 1242, 1380, 1588, 2415, 2595, 3294
BseRI GAGGAG 3 cut(s) 1799, 2197, 2846
BseXI GCAGC 4 cut(s) 190, 309, 2781, 3236
BseYI CCCAGC 2 cut(s) 1522, 2249
BsgI GTGCAG 4 cut(s) 452, 1552, 3119, 3306
Bsh1236I CGCG 1 cut(s) 3001
BshFI GGCC 2 cut(s) 2546, 3198
BsiHKAI GWGCWC 1 cut(s) 8
BsiSI CCGG 3 cut(s) 1504, 2135, 2324
BslFI GGGAC 1 cut(s) 1731
BslI CCNNNNNNNGG 3 cut(s) 1483, 1714, 2770
BsmAI GTCTC 1 cut(s) 1830
BsmFI GGGAC 1 cut(s) 1731
BsmI GAATGC 2 cut(s) 302, 2359
BsnI GGCC 2 cut(s) 2546, 3198
Bsp1286I GDGCHC 1 cut(s) 8
Bsp143I GATC 9 cut(s) 157, 343, 443, 1204, 1357, 1427, 2095, 2286, 2974
BspACI CCGC 6 cut(s) 643, 669, 1501, 2460, 2870, 3149
BspANI GGCC 2 cut(s) 2546, 3198
BspCNI CTCAG 5 cut(s) 60, 1813, 1884, 2469, 2895
BspFNI CGCG 1 cut(s) 3001
BspHI TCATGA 2 cut(s) 718, 3313
BspLI GGNNCC 4 cut(s) 853, 1706, 3113, 3292
BspMI ACCTGC 1 cut(s) 1518
BspPI GGATC 5 cut(s) 152, 338, 1199, 2281, 2982
BspTI CTTAAG 1 cut(s) 2645
BsrDI GCAATG 3 cut(s) 1326, 2415, 3178
BsrI ACTGG 7 cut(s) 1185, 1242, 1380, 1588, 2415, 2595, 3294
BssMI GATC 9 cut(s) 157, 343, 443, 1204, 1357, 1427, 2095, 2286, 2974
BssNI GRCGYC 2 cut(s) 639, 1557
Bst2UI CCWGG 3 cut(s) 2694, 2733, 2802
Bst6I CTCTTC 3 cut(s) 1071, 1237, 1299
BstACI GRCGYC 2 cut(s) 639, 1557
BstAFI CTTAAG 1 cut(s) 2645
BstAPI GCANNNNNTGC 2 cut(s) 200, 1471
BstC8I GCNNGC 2 cut(s) 394, 3147
BstDEI CTNAG 9 cut(s) 68, 1713, 1800, 1817, 1892, 2381, 2456, 2722, 2903
BstEII GGTNACC 1 cut(s) 1100
BstENI CCTNNNNNAGG 1 cut(s) 1712
BstFNI CGCG 1 cut(s) 3001
BstHHI GCGC 1 cut(s) 3001
BstKTI GATC 9 cut(s) 160, 346, 446, 1207, 1360, 1430, 2098, 2289, 2977
BstMAI GTCTC 1 cut(s) 1830
BstMBI GATC 9 cut(s) 157, 343, 443, 1204, 1357, 1427, 2095, 2286, 2974
BstMWI GCNNNNNNNGC 4 cut(s) 200, 824, 1471, 3177
BstNI CCWGG 3 cut(s) 2694, 2733, 2802
BstNSI RCATGY 2 cut(s) 1335, 1568
BstPI GGTNACC 1 cut(s) 1100
BstSCI CCNGG 4 cut(s) 1503, 2692, 2731, 2800
BstSFI CTRYAG 1 cut(s) 1620
BstUI CGCG 1 cut(s) 3001
BstV1I GCAGC 4 cut(s) 190, 309, 2781, 3236
BstV2I GAAGAC 5 cut(s) 101, 1151, 1560, 2427, 2769
BstX2I RGATCY 3 cut(s) 443, 1427, 2095
BstXI CCANNNNNNTGG 2 cut(s) 1215, 3326
BstYI RGATCY 3 cut(s) 443, 1427, 2095
Bsu36I CCTNAGG 1 cut(s) 1713
BsuI GTATCC 1 cut(s) 1776
BsuRI GGCC 2 cut(s) 2546, 3198
BtrI CACGTC 1 cut(s) 1645
BtsI GCAGTG 3 cut(s) 117, 2074, 2864
BtsIMutI CAGTG 7 cut(s) 53, 117, 1228, 2074, 2422, 2864, 2998
BveI ACCTGC 1 cut(s) 1518
Cac8I GCNNGC 2 cut(s) 394, 3147
CciI TCATGA 2 cut(s) 718, 3313
CfoI GCGC 1 cut(s) 3001
Cfr13I GGNCC 3 cut(s) 2886, 3112, 3290
CseI GACGC 1 cut(s) 2000
Csp6I GTAC 5 cut(s) 356, 1178, 1373, 2755, 3237
CspCI CAANNNNNGTGG 2 cut(s) 3005, 3040
CviQI GTAC 5 cut(s) 356, 1178, 1373, 2755, 3237
DdeI CTNAG 9 cut(s) 68, 1713, 1800, 1817, 1892, 2381, 2456, 2722, 2903
DpnI GATC 9 cut(s) 159, 345, 445, 1206, 1359, 1429, 2097, 2288, 2976
DpnII GATC 9 cut(s) 157, 343, 443, 1204, 1357, 1427, 2095, 2286, 2974
DraI TTTAAA 3 cut(s) 379, 1840, 1930
EaeI YGGCCR 1 cut(s) 2544
Eam1104I CTCTTC 3 cut(s) 1071, 1237, 1299
EarI CTCTTC 3 cut(s) 1071, 1237, 1299
EciI GGCGGA 1 cut(s) 1490
Eco147I AGGCCT 1 cut(s) 3198
Eco32I GATATC 1 cut(s) 3034
Eco47I GGWCC 3 cut(s) 2886, 3112, 3290
Eco57I CTGAAG 8 cut(s) 477, 827, 1128, 1323, 1611, 1709, 2421, 2974
Eco81I CCTNAGG 1 cut(s) 1713
Eco91I GGTNACC 1 cut(s) 1100
EcoNI CCTNNNNNAGG 1 cut(s) 1712
EcoO109I RGGNCCY 1 cut(s) 3290
EcoO65I GGTNACC 1 cut(s) 1100
EcoRI GAATTC 3 cut(s) 288, 481, 2237
EcoRII CCWGG 3 cut(s) 2692, 2731, 2800
EcoRV GATATC 1 cut(s) 3034
EcoT22I ATGCAT 2 cut(s) 256, 302
FalI AAGNNNNNCTT 2 cut(s) 1104, 1136
FaqI GGGAC 1 cut(s) 1731
FauI CCCGC 1 cut(s) 662
FblI GTMKAC 1 cut(s) 2476
Fnu4HI GCNGC 5 cut(s) 204, 323, 2795, 2870, 3225
Fsp4HI GCNGC 5 cut(s) 204, 323, 2795, 2870, 3225
FspBI CTAG 6 cut(s) 578, 990, 1295, 1322, 1457, 3231
GlaI GCGC 1 cut(s) 3000
GluI GCNGC 5 cut(s) 204, 323, 2795, 2870, 3225
GsaI CCCAGC 2 cut(s) 1526, 2253
GsuI CTGGAG 1 cut(s) 174
HaeIII GGCC 2 cut(s) 2546, 3198
HapII CCGG 3 cut(s) 1504, 2135, 2324
HgaI GACGC 1 cut(s) 2000
HhaI GCGC 1 cut(s) 3001
Hin1I GRCGYC 2 cut(s) 639, 1557
Hin6I GCGC 1 cut(s) 2999
HinP1I GCGC 1 cut(s) 2999
HincII GTYRAC 6 cut(s) 261, 1915, 1990, 2578, 2654, 2963
HindII GTYRAC 6 cut(s) 261, 1915, 1990, 2578, 2654, 2963
HindIII AAGCTT 4 cut(s) 1086, 1819, 2120, 2168
HpaI GTTAAC 1 cut(s) 2578
HpaII CCGG 3 cut(s) 1504, 2135, 2324
HphI GGTGA 6 cut(s) 92, 643, 1061, 2259, 2753, 3227
Hpy99I CGWCG 1 cut(s) 1292
HpyAV CCTTC 9 cut(s) 45, 749, 842, 851, 1030, 1595, 1958, 2883, 3209
HpyCH4IV ACGT 7 cut(s) 130, 639, 877, 1015, 1557, 1644, 2540
HpyF10VI GCNNNNNNNGC 4 cut(s) 200, 824, 1471, 3177
HpyF3I CTNAG 9 cut(s) 68, 1713, 1800, 1817, 1892, 2381, 2456, 2722, 2903
HpySE526I ACGT 7 cut(s) 130, 639, 877, 1015, 1557, 1644, 2540
Hsp92I GRCGYC 2 cut(s) 639, 1557
HspAI GCGC 1 cut(s) 2999
KspAI GTTAAC 1 cut(s) 2578
Kzo9I GATC 9 cut(s) 157, 343, 443, 1204, 1357, 1427, 2095, 2286, 2974
LmnI GCTCC 3 cut(s) 851, 1479, 1812
Lsp1109I GCAGC 4 cut(s) 190, 309, 2781, 3236
LweI GCATC 9 cut(s) 32, 263, 610, 1452, 2417, 2806, 3124, 3211, 3236
MaeI CTAG 6 cut(s) 578, 990, 1295, 1322, 1457, 3231
MaeII ACGT 7 cut(s) 130, 639, 877, 1015, 1557, 1644, 2540
MalI GATC 9 cut(s) 159, 345, 445, 1206, 1359, 1429, 2097, 2288, 2976
MboI GATC 9 cut(s) 157, 343, 443, 1204, 1357, 1427, 2095, 2286, 2974
MflI RGATCY 3 cut(s) 443, 1427, 2095
MhlI GDGCHC 1 cut(s) 8
MlsI TGGCCA 1 cut(s) 2546
MluNI TGGCCA 1 cut(s) 2546
MlyI GAGTC 6 cut(s) 1610, 1642, 1903, 2273, 2659, 2847
MmeI TCCRAC 2 cut(s) 562, 2952
Mox20I TGGCCA 1 cut(s) 2546
Mph1103I ATGCAT 2 cut(s) 256, 302
MroXI GAANNNNTTC 4 cut(s) 1088, 1116, 1316, 1728
MscI TGGCCA 1 cut(s) 2546
MslI CAYNNNNRTG 4 cut(s) 236, 1610, 2626, 3210
Msp20I TGGCCA 1 cut(s) 2546
MspA1I CMGCKG 1 cut(s) 2460
MspCI CTTAAG 1 cut(s) 2645
MspI CCGG 3 cut(s) 1504, 2135, 2324
MspR9I CCNGG 4 cut(s) 1505, 2694, 2733, 2802
Mva1269I GAATGC 2 cut(s) 302, 2359
MvaI CCWGG 3 cut(s) 2694, 2733, 2802
MvnI CGCG 1 cut(s) 3001
MwoI GCNNNNNNNGC 4 cut(s) 200, 824, 1471, 3177
NciI CCSGG 1 cut(s) 1505
NdeII GATC 9 cut(s) 157, 343, 443, 1204, 1357, 1427, 2095, 2286, 2974
NlaIV GGNNCC 4 cut(s) 853, 1706, 3113, 3292
NmuCI GTSAC 8 cut(s) 80, 240, 878, 1645, 1895, 2265, 2315, 2384
NsiI ATGCAT 2 cut(s) 256, 302
NspI RCATGY 2 cut(s) 1335, 1568
PagI TCATGA 2 cut(s) 718, 3313
PaqCI CACCTGC 1 cut(s) 1518
PceI AGGCCT 1 cut(s) 3198
PciI ACATGT 1 cut(s) 1564
PcsI WCGNNNNNNNCGW 2 cut(s) 1766, 1998
PctI GAATGC 2 cut(s) 302, 2359
PdmI GAANNNNTTC 4 cut(s) 1088, 1116, 1316, 1728
PflMI CCANNNNNTGG 1 cut(s) 1483
PfoI TCCNGGA 2 cut(s) 2692, 2731
PkrI GCNGC 5 cut(s) 205, 324, 2796, 2871, 3226
PleI GAGTC 6 cut(s) 1610, 1642, 1902, 2272, 2658, 2847
PpsI GAGTC 6 cut(s) 1610, 1642, 1902, 2272, 2658, 2847
PpuMI RGGWCCY 1 cut(s) 3290
PscI ACATGT 1 cut(s) 1564
PshBI ATTAAT 2 cut(s) 1887, 3183
PsiI TTATAA 1 cut(s) 1142
Psp5II RGGWCCY 1 cut(s) 3290
Psp6I CCWGG 3 cut(s) 2692, 2731, 2800
PspEI GGTNACC 1 cut(s) 1100
PspFI CCCAGC 2 cut(s) 1522, 2249
PspGI CCWGG 3 cut(s) 2692, 2731, 2800
PspN4I GGNNCC 4 cut(s) 853, 1706, 3113, 3292
PspPI GGNCC 3 cut(s) 2886, 3112, 3290
PspPPI RGGWCCY 1 cut(s) 3290
PsuI RGATCY 3 cut(s) 443, 1427, 2095
RsaI GTAC 5 cut(s) 357, 1179, 1374, 2756, 3238
RsaNI GTAC 5 cut(s) 356, 1178, 1373, 2755, 3237
RseI CAYNNNNRTG 4 cut(s) 236, 1610, 2626, 3210
SatI GCNGC 5 cut(s) 204, 323, 2795, 2870, 3225
Sau3AI GATC 9 cut(s) 157, 343, 443, 1204, 1357, 1427, 2095, 2286, 2974
Sau96I GGNCC 3 cut(s) 2886, 3112, 3290
ScaI AGTACT 2 cut(s) 1179, 1374
SchI GAGTC 6 cut(s) 1610, 1642, 1903, 2273, 2659, 2847
ScrFI CCNGG 4 cut(s) 1505, 2694, 2733, 2802
SduI GDGCHC 1 cut(s) 8
SfaNI GCATC 9 cut(s) 32, 263, 610, 1452, 2417, 2806, 3124, 3211, 3236
SfcI CTRYAG 1 cut(s) 1620
SinI GGWCC 3 cut(s) 2886, 3112, 3290
SmiMI CAYNNNNRTG 4 cut(s) 236, 1610, 2626, 3210
SmlI CTYRAG 2 cut(s) 592, 2645
SmoI CTYRAG 2 cut(s) 592, 2645
SseBI AGGCCT 1 cut(s) 3198
SsiI CCGC 6 cut(s) 643, 669, 1501, 2460, 2870, 3149
SspI AATATT 2 cut(s) 935, 1317
SspMI CTAG 6 cut(s) 578, 990, 1295, 1322, 1457, 3231
StuI AGGCCT 1 cut(s) 3198
StyD4I CCNGG 4 cut(s) 1503, 2692, 2731, 2800
TaiI ACGT 7 cut(s) 133, 642, 880, 1018, 1560, 1647, 2543
TaqI TCGA 8 cut(s) 210, 218, 604, 1041, 1360, 1812, 2001, 2780
TatI WGTACW 3 cut(s) 1177, 1372, 3236
TauI GCSGC 1 cut(s) 2872
TscAI CASTG 7 cut(s) 53, 124, 1228, 2074, 2422, 2871, 2998
TseFI GTSAC 8 cut(s) 80, 240, 878, 1645, 1895, 2265, 2315, 2384
TseI GCWGC 4 cut(s) 203, 322, 2794, 3224
Tsp45I GTSAC 8 cut(s) 80, 240, 878, 1645, 1895, 2265, 2315, 2384
TspGWI ACGGA 5 cut(s) 336, 751, 1758, 1759, 3104
TspRI CASTG 7 cut(s) 53, 124, 1228, 2074, 2422, 2871, 2998
Van91I CCANNNNNTGG 1 cut(s) 1483
Vha464I CTTAAG 1 cut(s) 2645
VpaK11BI GGWCC 3 cut(s) 2886, 3112, 3290
VspI ATTAAT 2 cut(s) 1887, 3183
XagI CCTNNNNNAGG 1 cut(s) 1712
XbaI TCTAGA 1 cut(s) 1294
XceI RCATGY 2 cut(s) 1335, 1568
XmiI GTMKAC 1 cut(s) 2476
XmnI GAANNNNTTC 4 cut(s) 1088, 1116, 1316, 1728
XspI CTAG 6 cut(s) 578, 990, 1295, 1322, 1457, 3231
ZraI GACGTC 2 cut(s) 640, 1558
ZrmI AGTACT 2 cut(s) 1179, 1374
Zsp2I ATGCAT 2 cut(s) 256, 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.