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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 158 |
| AccIII | TCCGGA | 1 cut(s) | 112 |
| AciI | CCGC | 2 cut(s) | 200, 324 |
| AclWI | GGATC | 1 cut(s) | 169 |
| AcoI | YGGCCR | 1 cut(s) | 57 |
| AcsI | RAATTY | 1 cut(s) | 399 |
| AcuI | CTGAAG | 1 cut(s) | 276 |
| AcyI | GRCGYC | 2 cut(s) | 5, 155 |
| AfaI | GTAC | 1 cut(s) | 351 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 85 |
| AgsI | TTSAA | 1 cut(s) | 217 |
| AjnI | CCWGG | 2 cut(s) | 73, 373 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 249, 281 |
| AluBI | AGCT | 2 cut(s) | 134, 347 |
| AluI | AGCT | 2 cut(s) | 134, 347 |
| AlwI | GGATC | 1 cut(s) | 169 |
| Ama87I | CYCGRG | 1 cut(s) | 187 |
| Aor13HI | TCCGGA | 1 cut(s) | 112 |
| AoxI | GGCC | 8 cut(s) | 57, 76, 236, 251, 301, 326, 341, 371 |
| ApeKI | GCWGC | 2 cut(s) | 24, 246 |
| ApoI | RAATTY | 1 cut(s) | 399 |
| AspS9I | GGNCC | 5 cut(s) | 110, 236, 326, 341, 371 |
| AsuC2I | CCSGG | 3 cut(s) | 188, 189, 240 |
| AsuHPI | GGTGA | 2 cut(s) | 163, 302 |
| AvaI | CYCGRG | 1 cut(s) | 187 |
| AvaII | GGWCC | 1 cut(s) | 110 |
| BalI | TGGCCA | 1 cut(s) | 59 |
| BbvI | GCAGC | 2 cut(s) | 11, 258 |
| BccI | CCATC | 2 cut(s) | 8, 301 |
| BceAI | ACGGC | 1 cut(s) | 316 |
| BciT130I | CCWGG | 2 cut(s) | 75, 375 |
| BcnI | CCSGG | 3 cut(s) | 188, 189, 240 |
| BfaI | CTAG | 2 cut(s) | 209, 359 |
| BisI | GCNGC | 2 cut(s) | 25, 247 |
| BlsI | GCNGC | 2 cut(s) | 26, 248 |
| Bme1390I | CCNGG | 5 cut(s) | 75, 188, 189, 240, 375 |
| Bme18I | GGWCC | 1 cut(s) | 110 |
| BmeT110I | CYCGRG | 1 cut(s) | 187 |
| BmgT120I | GGNCC | 5 cut(s) | 110, 236, 326, 341, 371 |
| BmiI | GGNNCC | 2 cut(s) | 111, 342 |
| BmrFI | CCNGG | 5 cut(s) | 75, 188, 189, 240, 375 |
| BmrI | ACTGGG | 1 cut(s) | 153 |
| BmsI | GCATC | 1 cut(s) | 373 |
| BmuI | ACTGGG | 1 cut(s) | 153 |
| BpmI | CTGGAG | 2 cut(s) | 18, 116 |
| BpuMI | CCSGG | 3 cut(s) | 188, 189, 240 |
| BsaHI | GRCGYC | 2 cut(s) | 5, 155 |
| BsaJI | CCNNGG | 3 cut(s) | 186, 187, 374 |
| BsaWI | WCCGGW | 1 cut(s) | 112 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 254, 284 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 85 |
| Bse1I | ACTGG | 1 cut(s) | 148 |
| BseAI | TCCGGA | 1 cut(s) | 112 |
| BseBI | CCWGG | 2 cut(s) | 75, 375 |
| BseDI | CCNNGG | 3 cut(s) | 186, 187, 374 |
| BseGI | GGATG | 1 cut(s) | 316 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 85 |
| BseMII | CTCAG | 1 cut(s) | 75 |
| BseNI | ACTGG | 1 cut(s) | 148 |
| BseRI | GAGGAG | 1 cut(s) | 21 |
| BseXI | GCAGC | 2 cut(s) | 11, 258 |
| BseYI | CCCAGC | 1 cut(s) | 106 |
| BsgI | GTGCAG | 2 cut(s) | 10, 318 |
| BshFI | GGCC | 8 cut(s) | 59, 78, 238, 253, 303, 328, 343, 373 |
| BsiHKCI | CYCGRG | 1 cut(s) | 187 |
| BsiSI | CCGG | 3 cut(s) | 113, 188, 239 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 85 |
| BsnI | GGCC | 8 cut(s) | 59, 78, 238, 253, 303, 328, 343, 373 |
| BsoBI | CYCGRG | 1 cut(s) | 187 |
| Bsp13I | TCCGGA | 1 cut(s) | 112 |
| Bsp1407I | TGTACA | 1 cut(s) | 349 |
| Bsp143I | GATC | 1 cut(s) | 161 |
| BspACI | CCGC | 2 cut(s) | 200, 324 |
| BspANI | GGCC | 8 cut(s) | 59, 78, 238, 253, 303, 328, 343, 373 |
| BspCNI | CTCAG | 1 cut(s) | 76 |
| BspEI | TCCGGA | 1 cut(s) | 112 |
| BspLI | GGNNCC | 2 cut(s) | 111, 342 |
| BspPI | GGATC | 1 cut(s) | 169 |
| BsrGI | TGTACA | 1 cut(s) | 349 |
| BsrI | ACTGG | 1 cut(s) | 148 |
| BssECI | CCNNGG | 3 cut(s) | 186, 187, 374 |
| BssMI | GATC | 1 cut(s) | 161 |
| BssNI | GRCGYC | 2 cut(s) | 5, 155 |
| Bst2UI | CCWGG | 2 cut(s) | 75, 375 |
| Bst4CI | ACNGT | 1 cut(s) | 169 |
| BstACI | GRCGYC | 2 cut(s) | 5, 155 |
| BstAUI | TGTACA | 1 cut(s) | 349 |
| BstC8I | GCNNGC | 4 cut(s) | 177, 251, 326, 345 |
| BstDEI | CTNAG | 1 cut(s) | 84 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 83 |
| BstF5I | GGATG | 1 cut(s) | 316 |
| BstKTI | GATC | 1 cut(s) | 164 |
| BstMBI | GATC | 1 cut(s) | 161 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 44 |
| BstNI | CCWGG | 2 cut(s) | 75, 375 |
| BstSCI | CCNGG | 5 cut(s) | 73, 186, 187, 238, 373 |
| BstV1I | GCAGC | 2 cut(s) | 11, 258 |
| BstX2I | RGATCY | 1 cut(s) | 161 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 403 |
| BstYI | RGATCY | 1 cut(s) | 161 |
| BsuRI | GGCC | 8 cut(s) | 59, 78, 238, 253, 303, 328, 343, 373 |
| BtsCI | GGATG | 1 cut(s) | 316 |
| Cac8I | GCNNGC | 4 cut(s) | 177, 251, 326, 345 |
| Cfr13I | GGNCC | 5 cut(s) | 110, 236, 326, 341, 371 |
| Cfr9I | CCCGGG | 1 cut(s) | 187 |
| CseI | GACGC | 1 cut(s) | 168 |
| Csp6I | GTAC | 1 cut(s) | 350 |
| CviAII | CATG | 1 cut(s) | 353 |
| CviQI | GTAC | 1 cut(s) | 350 |
| DdeI | CTNAG | 1 cut(s) | 84 |
| DpnI | GATC | 1 cut(s) | 163 |
| DpnII | GATC | 1 cut(s) | 161 |
| EaeI | YGGCCR | 1 cut(s) | 57 |
| Eco147I | AGGCCT | 1 cut(s) | 253 |
| Eco47I | GGWCC | 1 cut(s) | 110 |
| Eco57I | CTGAAG | 1 cut(s) | 276 |
| Eco88I | CYCGRG | 1 cut(s) | 187 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 83 |
| EcoRII | CCWGG | 2 cut(s) | 73, 373 |
| FaeI | CATG | 1 cut(s) | 356 |
| FaiI | YATR | 3 cut(s) | 137, 284, 354 |
| FatI | CATG | 1 cut(s) | 352 |
| FauI | CCCGC | 2 cut(s) | 193, 317 |
| Fnu4HI | GCNGC | 2 cut(s) | 25, 247 |
| FokI | GGATG | 1 cut(s) | 323 |
| Fsp4HI | GCNGC | 2 cut(s) | 25, 247 |
| FspBI | CTAG | 2 cut(s) | 209, 359 |
| GluI | GCNGC | 2 cut(s) | 25, 247 |
| GsaI | CCCAGC | 1 cut(s) | 110 |
| GsuI | CTGGAG | 2 cut(s) | 18, 116 |
| HaeIII | GGCC | 8 cut(s) | 59, 78, 238, 253, 303, 328, 343, 373 |
| HapII | CCGG | 3 cut(s) | 113, 188, 239 |
| HgaI | GACGC | 1 cut(s) | 168 |
| Hin1I | GRCGYC | 2 cut(s) | 5, 155 |
| Hin1II | CATG | 1 cut(s) | 356 |
| HincII | GTYRAC | 1 cut(s) | 273 |
| HindII | GTYRAC | 1 cut(s) | 273 |
| HinfI | GANTC | 2 cut(s) | 68, 409 |
| HpaII | CCGG | 3 cut(s) | 113, 188, 239 |
| HphI | GGTGA | 2 cut(s) | 163, 302 |
| Hpy166II | GTNNAC | 1 cut(s) | 273 |
| Hpy188I | TCNGA | 1 cut(s) | 85 |
| Hpy188III | TCNNGA | 3 cut(s) | 9, 113, 159 |
| Hpy8I | GTNNAC | 1 cut(s) | 273 |
| HpyAV | CCTTC | 1 cut(s) | 251 |
| HpyCH4III | ACNGT | 1 cut(s) | 169 |
| HpyCH4IV | ACGT | 1 cut(s) | 155 |
| HpyCH4V | TGCA | 3 cut(s) | 27, 335, 365 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 44 |
| HpyF3I | CTNAG | 1 cut(s) | 84 |
| HpySE526I | ACGT | 1 cut(s) | 155 |
| Hsp92I | GRCGYC | 2 cut(s) | 5, 155 |
| Hsp92II | CATG | 1 cut(s) | 356 |
| Kpn2I | TCCGGA | 1 cut(s) | 112 |
| Kzo9I | GATC | 1 cut(s) | 161 |
| Lsp1109I | GCAGC | 2 cut(s) | 11, 258 |
| LweI | GCATC | 1 cut(s) | 373 |
| MaeI | CTAG | 2 cut(s) | 209, 359 |
| MaeII | ACGT | 1 cut(s) | 155 |
| MaeIII | GTNAC | 2 cut(s) | 151, 296 |
| MalI | GATC | 1 cut(s) | 163 |
| MboI | GATC | 1 cut(s) | 161 |
| MflI | RGATCY | 1 cut(s) | 161 |
| MlsI | TGGCCA | 1 cut(s) | 59 |
| MluCI | AATT | 2 cut(s) | 63, 399 |
| MluNI | TGGCCA | 1 cut(s) | 59 |
| MlyI | GAGTC | 2 cut(s) | 77, 403 |
| MnlI | CCTC | 6 cut(s) | 42, 79, 91, 109, 121, 256 |
| Mox20I | TGGCCA | 1 cut(s) | 59 |
| MroI | TCCGGA | 1 cut(s) | 112 |
| MscI | TGGCCA | 1 cut(s) | 59 |
| Msp20I | TGGCCA | 1 cut(s) | 59 |
| MspA1I | CMGCKG | 1 cut(s) | 347 |
| MspI | CCGG | 3 cut(s) | 113, 188, 239 |
| MspR9I | CCNGG | 5 cut(s) | 75, 188, 189, 240, 375 |
| MvaI | CCWGG | 2 cut(s) | 75, 375 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 44 |
| NciI | CCSGG | 3 cut(s) | 188, 189, 240 |
| NdeII | GATC | 1 cut(s) | 161 |
| NlaIII | CATG | 1 cut(s) | 356 |
| NlaIV | GGNNCC | 2 cut(s) | 111, 342 |
| NmuCI | GTSAC | 2 cut(s) | 151, 296 |
| PceI | AGGCCT | 1 cut(s) | 253 |
| PkrI | GCNGC | 2 cut(s) | 26, 248 |
| PleI | GAGTC | 2 cut(s) | 76, 403 |
| PpsI | GAGTC | 2 cut(s) | 76, 403 |
| Psp6I | CCWGG | 2 cut(s) | 73, 373 |
| PspFI | CCCAGC | 1 cut(s) | 106 |
| PspGI | CCWGG | 2 cut(s) | 73, 373 |
| PspN4I | GGNNCC | 2 cut(s) | 111, 342 |
| PspPI | GGNCC | 5 cut(s) | 110, 236, 326, 341, 371 |
| PsuI | RGATCY | 1 cut(s) | 161 |
| PvuII | CAGCTG | 1 cut(s) | 347 |
| RsaI | GTAC | 1 cut(s) | 351 |
| RsaNI | GTAC | 1 cut(s) | 350 |
| SatI | GCNGC | 2 cut(s) | 25, 247 |
| Sau3AI | GATC | 1 cut(s) | 161 |
| Sau96I | GGNCC | 5 cut(s) | 110, 236, 326, 341, 371 |
| SchI | GAGTC | 2 cut(s) | 77, 403 |
| ScrFI | CCNGG | 5 cut(s) | 75, 188, 189, 240, 375 |
| SetI | ASST | 7 cut(s) | 102, 136, 158, 210, 267, 289, 349 |
| SfaNI | GCATC | 1 cut(s) | 373 |
| SinI | GGWCC | 1 cut(s) | 110 |
| SmaI | CCCGGG | 1 cut(s) | 189 |
| Sse9I | AATT | 2 cut(s) | 63, 399 |
| SseBI | AGGCCT | 1 cut(s) | 253 |
| SsiI | CCGC | 2 cut(s) | 200, 324 |
| SspMI | CTAG | 2 cut(s) | 209, 359 |
| StuI | AGGCCT | 1 cut(s) | 253 |
| StyD4I | CCNGG | 5 cut(s) | 73, 186, 187, 238, 373 |
| TaaI | ACNGT | 1 cut(s) | 169 |
| TaiI | ACGT | 1 cut(s) | 158 |
| TasI | AATT | 2 cut(s) | 63, 399 |
| TatI | WGTACW | 1 cut(s) | 349 |
| TseFI | GTSAC | 2 cut(s) | 151, 296 |
| TseI | GCWGC | 2 cut(s) | 24, 246 |
| Tsp45I | GTSAC | 2 cut(s) | 151, 296 |
| TspGWI | ACGGA | 1 cut(s) | 140 |
| TspMI | CCCGGG | 1 cut(s) | 187 |
| VpaK11BI | GGWCC | 1 cut(s) | 110 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 83 |
| XapI | RAATTY | 1 cut(s) | 399 |
| XmaI | CCCGGG | 1 cut(s) | 187 |
| XspI | CTAG | 2 cut(s) | 209, 359 |
| ZraI | GACGTC | 1 cut(s) | 156 |